Chong et al., 2011 - Google Patents

Line-scan focal modulation microscopy for rapid imaging of thick biological specimens

Chong et al., 2011

Document ID
14629586037215126565
Author
Chong S
Pant S
Chen N
Publication year
Publication venue
2011 Asia Communications and Photonics Conference and Exhibition (ACP)

External Links

Snippet

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal Modulation Microscopy [1-3](FMM) is an …
Continue reading at ieeexplore.ieee.org (other versions)

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0028—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders specially adapted for specific applications, e.g. for endoscopes, ophthalmoscopes, attachments to conventional microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/10—Condensers affording dark-field illumination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultra-violet illumination; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques

Similar Documents

Publication Publication Date Title
Chong et al. Line-scan focal modulation microscopy for rapid imaging of thick biological specimens
Baumgart et al. Scanned light sheet microscopy with confocal slit detection
Mertz Optical sectioning microscopy with planar or structured illumination
Shao et al. Super-resolution 3D microscopy of live whole cells using structured illumination
Thériault et al. Extended two-photon microscopy in live samples with Bessel beams: steadier focus, faster volume scans, and simpler stereoscopic imaging
Truong et al. Deep and fast live imaging with two-photon scanned light-sheet microscopy
US11530990B2 (en) Light-sheet microscope with parallelized 3D image acquisition
WO2014007763A1 (en) Light microscope and method of controlling the same
Hedde et al. Selective plane illumination microscopy with a light sheet of uniform thickness formed by an electrically tunable lens
JP2021165851A (en) Configuration for light sheet microscopy, and method therefor
US8198604B2 (en) System and method for providing enhanced background rejection in thick tissue with differential-aberration two-photon microscopy
Westphal et al. Dynamic far-field fluorescence nanoscopy
CN111273433A (en) High-speed large-field-of-view digital scanning light-sheet microscopic imaging system
US10914930B2 (en) Stimulated emission depletion nonlinear structured illumination microscopy (STED-NSIM) apparatus, methods, and applications
Qu et al. Recent progress in multifocal multiphoton microscopy
Deguchi et al. Volumetric Lissajous confocal microscopy with tunable spatiotemporal resolution
JP6928757B2 (en) Systems and methods for image processing in light microscopy
Oron et al. Temporal focusing microscopy
KR20210086694A (en) High Flux Optical Tomography 3D Imaging System
Wang et al. High-speed 3D imaging based on structured illumination and electrically tunable lens
Pant et al. Line-Scan Focal Modulation Microscopy for Rapid Imaging of Thick Biological Tissues
KR101075155B1 (en) Apparatus and method for confocal laser scanning microscopy system using line scanning way
KR20110018718A (en) Confocal laser scanning microscopy applying two direction line scanning way and image processing method using thereof
Tadesse et al. 3D Multifocal Scanning Microscopy for Super-Resolution Cell and Tissue Imaging
Hellerer et al. Super-resolution upgrade for deep tissue imaging featuring easy implementation