WO2016029647A1

WO2016029647A1 - Active seaweed feed additive and preparation method therefor

Info

Publication number: WO2016029647A1
Application number: PCT/CN2015/071861
Authority: WO
Inventors: 李健; 李明潭
Original assignee: 威海市世代海洋生物科技有限公司
Priority date: 2014-08-26
Filing date: 2015-01-29
Publication date: 2016-03-03
Also published as: CN106615663A; CN104146150A

Abstract

A seaweed biological feed additive or a preparation method for a seaweed biological feed. The method is characterized by adding a composite microorganism enzyme into seaweed serosity that is prepared from seaweed or seaweed residues, and performing enzymolysis to obtain the seaweed biological feed additive, wherein the composite microorganism enzyme consists of several kinds of cellulase, pectinase, protease and amylase.

Description

Active seaweed feed additive and preparation method thereof

Technical field

The invention relates to the field of feed production, in particular to an active seaweed feed additive which is enzymatically hydrolyzed by a composite enzyme and a preparation method thereof.

Background technique

As a big agricultural country and a big aquatic country, aquatic products have become an important animal protein consumption product, and it has grown rapidly every year. With the increasing consumption level of aquatic products, it has greatly stimulated the rapid development of China's aquaculture industry, especially the aquaculture with seaweed as a food source, such as abalone and sea cucumber. Seaweed plants are rich in nutrients and have functions such as medicinal and health care. At the same time, seaweed plants are gradually entering people's production and life as important consumables, and are widely used in the fields of industry, agriculture and medicine. This is because seaweed is a self-supporting plant that can perform photosynthesis and convert inorganic matter in the ocean into organic matter. Therefore, seaweed is a rich and low-cost feed resource for animals. Seaweed has great potential for development as a feed ingredient and feed additive. In addition, seaweed is rich in seaweed polysaccharides, protein, fat, vitamins, minerals and physiologically active substances with special effects. It is a raw material library for food, medicine and feed. Marine plants are rich in nutrients and contain a variety of biologically active substances, which have biological activities such as enhancing immunity, disease resistance, anti-virus, and growth. Therefore, the development of marine plant feed and the promotion of animal husbandry are receiving increasing attention.

However, in recent years, with the rapid increase in population and the large reduction of cultivated land, the shortage of feed has become a serious problem affecting the rapid development of the aquaculture industry, and the contradiction between people and animals for food has become more serious. Because seaweed contains a variety of anti-nutritional ingredients, such as cellulose, sugar gum, agar, xylan and other non-starch polysaccharides and their toxins; plus aquatic animals eating seaweed are mostly lower animals, their intestinal tract is short, digestive function is poor Other factors have greatly affected the digestion and utilization of seaweed, resulting in waste of resources, and also easily cause various discomforts in aquatic animals, such as enteritis and swollen mouth. In addition, due to the late start of aquaculture in China, the systematic research is lagging behind, the farming technology is imperfect, and it is currently in an extensive farming mode. Therefore, feeding feed is mostly operated by feeling or experience, resulting in serious feed waste and nutrient loss. Causes the water environment and the deterioration of the bottom of the pool. Therefore, how to improve the utilization rate of seaweed and its nutrient level, and reduce the toxin is urgently needed to be solved. problem.

Faced with the above problems, Chinese patent CN 102987077 A discloses a preparation method of seaweed fermented feed, which improves the nutritive value that seaweed can absorb. However, the preparation method still has the defects of complicated preparation process, long preparation time and high cost, which is far from meeting the market demand.

Summary of the invention

In view of the deficiencies of the prior art, the present invention provides a method for preparing a seaweed biological feed additive, which is characterized in that the method is to add a microbial enzyme to an algae slurry prepared from seaweed or algae residue to obtain an enzymatic hydrolysis solution. The seaweed biological feed, wherein the composite microbial enzyme is composed of a plurality of cellulase, pectinase, protease, and amylase.

According to a preferred embodiment, the mass percentage of the cellulase, the pectinase, the protease and the amylase in the complex microbial enzyme are: 60-100%, 0-40%, respectively. 0 to 5%, 0 to 5%, and the cellulase is derived from Trichoderma reesei, Trichoderma viride, and/or Aspergillus niger; the pectinase is derived from Rhizopus oryzae, Aspergillus niger and/or rice Aspergillus; the protease is derived from papain, bromelain and/or Bacillus subtilis; the amylase is derived from Bacillus licheniformis, Aspergillus oryzae and/or Aspergillus niger.

According to a preferred embodiment, the enzyme activity of the cellulase is (18-24)×10 ⁴ μ/g, and the enzyme activity of the pectinase is (2-5)×10 ⁴ μ/g. The protease activity of the protease is (10 to 50) × 10 ⁴ μ / g, and the enzyme activity of the amylase is (1 to 5) × 10 ⁴ μ / g.

According to a preferred embodiment, the method comprises adding a composite microbial enzyme to the seaweed slurry according to a mass ratio of the composite microbial enzyme to the seaweed slurry of (0.3 to 3):100, and the normal pressure and the temperature are 30 ～. The seaweed biological feed was obtained by insulating the enzyme for 6 to 30 hours under conditions of 65 ° C and pH 3 to 7.

According to a preferred embodiment, the method further comprises the steps of: shearing and grinding the seaweed or seaweed slag into an algae slurry having particles having a diameter of 1 mm or less after inorganic decontamination; and driving the seaweed slurry into a high-speed dispersing kettle Mixing with water once, mixing the water with the kelp slurry, and the mass ratio of the first water addition to the kelp slurry is (30-40): 100; the mixed seaweed slurry is put into the enzymatic hydrolysis tank, the first Adding water twice and adding the composite microbial enzyme to form the mixed liquid, and the mass ratio of the total amount of the first water addition amount and the second water addition amount to the seaweed slurry is 50:100; and the mixing The liquid was stirred uniformly, and then heat-insulated and enzymatically hydrolyzed, and stirred once every hour during the enzymatic hydrolysis, and stirred for 5 minutes each time.

According to a preferred embodiment, the enzymatically prepared mixture is subjected to solid-liquid separation by a backflushing belt filter, and the separated enzyme solution is concentrated in a range of 60 to 68 ° C; or, the enzymatic hydrolysis is carried out. A good mixture is dried.

According to a preferred embodiment, the rotational speed of the high-speed dispersing vessel after starting is 3000 rpm, and after 3 minutes of agitation, the rotational speed of the high-speed dispersing vessel is reduced from 3000 rpm to 1000 rpm.

In another aspect of the invention, a seaweed biological feed additive is characterized in that the seaweed biological feed is prepared according to the preparation method as described above.

According to a preferred embodiment, the seaweed feed further comprises one or more of fish meal, fish oil, soybean meal, tapioca starch, corn flour and powdered soybean phospholipid.

In another aspect of the invention, a seaweed biological feed, in particular for feeding poultry/livestock or aquaculture, characterized in that the feed comprises a seaweed biological feed additive prepared as described above, or as The aforementioned seaweed feed additive.

The invention has the advantages of simple preparation method, easy operation and control, mild preparation process condition, short preparation period and low cost. The invention improves the utilization rate of seaweed by adding a composite microbial enzyme to the slurry prepared from seaweed or seaweed residue, and retains more active nutrients in the seaweed, and the active nutrient component in the seaweed is not affected in the mild enzymatic hydrolysis process. Destruction is conducive to the digestion and absorption of active nutrients by animals and promotes the growth of animals.

DRAWINGS

1 is a comparison diagram of hydrolysis effects of cellulase produced by different strains in the preparation method of seaweed feed additive of the present invention;

2 is a comparison diagram of hydrolysis effects of pectinase produced by different strains in the preparation method of seaweed feed additive of the present invention;

3 is a comparison diagram of hydrolysis effects of amylase produced by different strains in the preparation method of seaweed feed additive of the present invention;

4 is a comparison diagram of hydrolysis effects of proteases produced by different strains in the preparation method of seaweed feed additive of the present invention;

Figure 5 is a graph showing the effect of the amount of the compound microbial enzyme on the hydrolysis effect in the preparation method of the seaweed feed additive of the present invention;

Fig. 6 is a graph showing the effect of the enzymatic hydrolysis time of the composite microbial enzyme on the hydrolysis effect in the preparation method of the seaweed feed additive of the present invention.

detailed description

Detailed description will be made below with reference to the accompanying drawings and embodiments.

The method for preparing the seaweed biological feed additive or the seaweed feed of the present invention, wherein the method is to add a microbial enzyme to the seaweed slurry prepared from seaweed or algae residue to carry out enzymatic hydrolysis to obtain the seaweed biological feed, wherein The complex microbial enzyme consists of a plurality of cellulases, pectinase, proteases and amylases.

1 selection of enzymes

In order to obtain an enzyme species constituting a composite microbial enzyme having the best hydrolysis effect, the present invention uses a cellulase produced by Trichoderma reesei, Trichoderma viride, and Aspergillus niger as a fiber. Alternative to the enzyme; the pectinase produced by Rhizopus oryzae, Aspergillus niger, Aspergillus oryzae is an alternative to pectinase; Caracapapaya protease Papain, pineapple (Ananas spp) protease (Bromelain), Bacillus subtilis protease is an alternative to protease; amylase produced by Bacillus licheniformis, Aspergillus oryzae, Aspergillus niger An alternative to amylase. The mold was selected by studying the hydrolysis effect of cellulase or pectinase or protease or amylase produced by each mold on seaweed, and the hydrolysis effect of various enzymes on seaweed was evaluated by the content of alginic acid. The enzyme activities and optimal enzymatic conditions of the above-mentioned molds selected for use in the present invention are shown in Table 1.

Table 1

Cellulase, pectinase, protease and amylase from different sources in Table 1 were subjected to hydrolysis experiments, and the test results are shown in Figures 1 to 4.

Figure 1 shows the hydrolysis effect of cellulase produced by Trichoderma reesei, Trichoderma viride and Aspergillus niger. The hydrolysis effect is based on the amount of alginic acid released. Among them, the cellulase produced by Trichoderma reesei has the best hydrolysis effect, and with the enzyme addition amount from 1‰ to 5‰, the hydrolyzed alginic acid content is from 8g/L to 22g/L, which is produced by Trichoderma viride. The cellulase is added from 1‰ to 5‰ with the enzyme, and the alginic acid content of the hydrolyzed acid is from 9g/L to 19.5g/L. The cellulase produced by Aspergillus niger is hydrolyzed from 1‰ to 5‰. The alginic acid content is from 7 g/L to 17 g/L. Therefore, in the present invention, the cellulase produced by Trichoderma reesei is preferably used as the cellulase in the complex enzyme. Of course, cellulase produced by Trichoderma viride and Aspergillus niger is also feasible.

Figure 2 shows the hydrolysis effect of pectinase produced by Aspergillus niger, Aspergillus oryzae and Rhizopus oryzae. The hydrolysis effect is based on the amount of alginic acid released. Among them, the hydrolysis of pectinase produced by Rhizopus oryzae The effect is best, with the amount of enzyme added from 1‰ to 5‰, the hydrolyzed alginic acid content is 6.5g/L～11.6g/L, produced by Aspergillus niger The pectinase is added from 1‰ to 5‰ with the enzyme, and the hydrolyzed alginic acid content is 5.9g/L～11g/L. The pectinase produced by Aspergillus oryzae is from 1‰～5‰, and the hydrolyzed seaweed The acid content is from 6.1 g/L to 13 g/L. Therefore, in the present invention, pectinase produced by Rhizopus oryzae is preferably used as a pectinase in the complex enzyme. Of course, it is also feasible to use pectinase produced by Aspergillus niger and Aspergillus oryzae.

Figure 3 shows the hydrolysis effect of subtilisin, papain, and bromelain. The hydrolysis effect is based on the amount of alginic acid released. Among them, the hydrolysis effect of bromelain is the best, with the enzyme addition amount from 0.5‰ to 2.5. The hydrolyzed alginic acid content is 5.58 g/L to 6.8 g/L. When the amount is more than 1 Torr, the hydrolyzed alginic acid content is reduced to 6.7 g/L and tends to be balanced. The hydrolysis effect of papain is from 0.5 ‰ to 2.5 酶, and the hydrolyzed alginic acid content is 5.89 g/L to 6.6 g/L. The hydrolysis effect of subtilisin is from 0.5 ‰ to 2.5 酶, and the hydrolyzed alginic acid content is from 5.78 g/L to 6.2 g/L. Therefore, in the present invention, bromelain is preferred as a protease in the complex enzyme. Of course, it is also possible to use subtilisin and papain.

Figure 4 shows the hydrolysis effect of amylase produced by Aspergillus niger, Aspergillus oryzae, Bacillus licheniformis, etc. The hydrolysis effect is based on the amount of alginic acid released. Among them, the hydrolysis effect of amylase produced by Bacillus licheniformis is the most Well, with the enzyme addition amount from 1‰ to 5‰, the hydrolyzed alginic acid content is 5.77g/L to 6.2g/L, and the amylase produced by Aspergillus oryzae is hydrolyzed with the enzyme addition amount from 1‰ to 5‰. The alginic acid content is 5.6g/L to 5.88g/L, and the amylase produced by Aspergillus niger is from 1‰ to 5‰, and the hydrolyzed alginic acid content is from 5.55g/L to 5.78g/L. Therefore, in the present invention, the amylase produced by Bacillus licheniformis is preferred as the amylase in the complex enzyme, and of course, the amylase produced by Aspergillus niger and Aspergillus oryzae is also feasible.

In summary, a preferred embodiment of the present invention is to select a cellulase produced by Trichoderma reesei as a cellulase in a composite microbial enzyme, and select a pectinase produced by Rhizopus oryzae as a composite microbial enzyme. Pectinase, bromelain was selected as the protease in the composite microbial enzyme, and the amylase produced by Bacillus licheniformis was selected as the amylase in the composite microbial enzyme. Moreover, the composite microbial enzyme of the present invention is mainly cellulase, followed by pectinase, followed by protease, and amylase. The single enzyme source and properties of the preferred composite microorganisms of the present invention are shown in Table 2.

Table 2

2 compounding design of compound microbial enzyme

The present invention carried out the enzymatic hydrolysis experiments of Examples 1 to 6 in accordance with the ratios in Table 3.

table 3

Example 1

A composite microbial enzyme of the present invention consisting of 100% cellulase, wherein the cellulase enzyme activity selected is 200,000 μg/g. 100 ml of kelp slurry was added, and the mixed microbial enzyme was added in a ratio of 0.4% by mass of the slurry prepared from seaweed or seaweed residue to form a mixed solution, and the enzymatic hydrolysis was carried out for 25 hours, and the content of alginic acid in the supernatant was determined to be 17.25 g. /L.

Example 2

The invention relates to a composite microbial enzyme which is prepared by mixing 60% by mass of cellulase and 40% of pectinase, wherein the cellulase activity of the selected cellulase is 120,000 μg/g, pectin. The enzyme activity of the enzyme was 12,000 μg/g. Take 100ml of kelp slurry, according to the mass ratio of the slurry made from seaweed or seaweed residue The composite microbial enzyme was added in a ratio of 0.4% to form a mixed solution, and the enzymatic hydrolysis was carried out for 25 hours, and the content of alginic acid in the supernatant was determined to be 19.56 g/L.

Example 3

The invention relates to a composite microbial enzyme which is prepared by mixing 60% by mass of cellulase with 35% of pectinase and 5% of protease, wherein the cellulase activity of the selected cellulase is 120,000 μ. /g, the pectinase activity was 1.05 million μg/g, and the protease activity was 25,000 μg/g. 100ml of kelp slurry was added, and the mixed microbial enzyme was added in a ratio of 0.4% by mass of the slurry prepared from seaweed or seaweed residue to form a mixed solution, and the enzymatic hydrolysis was carried out for 25 hours, and the content of alginic acid in the supernatant was determined to be 21.32 g. /L.

Example 4

The invention relates to a composite microbial enzyme which is prepared by mixing 60% by mass of cellulase and 35% of pectinase and 5% of amylase, wherein the cellulase activity of the selected cellulase is 120,000. μ, the pectinase activity was 1.05 million μg/g, and the amylase enzyme activity was 0.2 million μg/g. 100 ml of kelp slurry was added, and the composite microbial enzyme was added in a ratio of 0.4% by mass of the slurry prepared from seaweed or seaweed residue to form a mixed solution, and the enzymatic hydrolysis was carried out for 25 hours, and the content of alginic acid in the supernatant was determined to be 19.62 g. /L.

Example 5

The composite microbial enzyme of the invention is prepared by mixing 70% by mass of cellulase and 27% of pectinase and 3% of protease, wherein the cellulase activity of the selected cellulase is 140,000 μ. The enzyme activity of the pectinase was 0.81 μg/g, and the enzyme activity of the protease was 15,000 μg/g. 100 ml of kelp slurry was added, and the mixed microbial enzyme was added in a ratio of 0.4% by mass of the slurry prepared from seaweed or seaweed residue to form a mixed solution, and the enzymatic hydrolysis was carried out for 25 hours, and the content of alginic acid in the supernatant was determined to be 22.35 g. /L.

Example 6

A composite microbial enzyme of the present invention which is prepared by mixing 60% by mass of cellulase with 30% of pectinase, 5% of protease and 5% of amylase, wherein cellulase is selected. The enzyme activity was 120,000 μg, the pectinase activity was 0.81 μg/g, the protease activity was 25,000 μg/g, and the amylase enzyme activity was 0.2 million μg/g. 100 ml of kelp slurry was added, and the composite microbial enzyme was added in a ratio of 0.4% by mass of the slurry prepared from seaweed or seaweed residue to form a mixed solution, and the enzymatic hydrolysis was carried out for 25 hours, and the content of alginic acid in the supernatant was determined to be 24.46 g. /L.

Combining the compound microbial enzymes of Examples 1 to 6 to degrade algae (the enzymatic hydrolysis effect is based on the amount of released alginic acid), it can be seen that the enzymatic hydrolysis effect of the composite microbial enzyme on fresh seaweed is better than using a single enzyme. The effect is good. In addition to cellulose and crude protein, fresh seaweed also contains kelp polysaccharides, which are alginate, fucoidan and brown algae starch. Among them, brown algae starch is an intracellular polysaccharide mainly composed of β-1,3-D-glucan. There are a small amount of β-1,6 interchain glycosidic bonds on the long chain, and the content in kelp is generally 1%. about. Therefore, the use of amylase as an auxiliary enzyme in the complex microbial enzyme can make the fresh kelp more fully enzymatically hydrolyzed. In addition, the content of crude protein in kelp is 5 to 9%, and the content is relatively low. From Table 2, it can be seen that the price of protease is relatively high. If the amount of protease used is equivalent to other enzymes, the processing cost will increase. Causes waste of resources. Therefore, the mass percentage of cellulase and pectinase used in the present invention is much larger than that of protease and amylase, and the basic enzymes using cellulase and pectinase as composite microbial enzymes, and protease and amylase as complex microbial enzymes are determined. Auxiliary enzyme species. Table 3 shows that the composite microbial enzyme composed of Example 6 has the best enzymatic hydrolysis effect on the kelp slurry. Example 6 was therefore taken as the preferred embodiment of the complex microbial enzyme ratio.

3The effect of the amount of compound microbial enzyme on the hydrolysis

The composite microbial enzyme consisting of the optimal ratio in Example 6 was used to study the effect of the amount of the combined microbial enzyme on the hydrolysis effect of seaweed. Its hydrolysis effect is shown in Figure 5.

The alginic acid content gradually increased with the addition of the complex microbial enzyme from 0 to 0.6%; and the alginic acid content increased substantially linearly with the addition of the complex microbial enzyme from 0 to 0.3%; When the added amount is 0.3%, the alginic acid content is 17.5g/L; when the compounding enzyme reaches 0.4%, the alginic acid content reaches 18g/L; when the compounding enzyme is added more than 0.4%, the alginic acid content tends to Balance, no longer increase. Therefore, the amount of the complex microbial enzyme to be added of the present invention is preferably from 0.3 to 0.4% in view of overall cost and time.

4 Determination of enzymatic process parameters

Table 4 lists parameters such as reaction temperature, enzyme addition amount, pH value and enzymatic hydrolysis time in the enzymatic hydrolysis process of the composite microorganism of the present invention. Table 5 lists the results of Examples 7-15 of the orthogonal test according to the parameters shown in Table 4. The present invention determines the preferred process parameters of the present invention from Tables 4 and 5.

Table 4

table 5

2700ml fresh seaweed homogenate was taken and packed into 27 250ml triangular flasks, each containing 100ml, three groups were tested according to the orthogonal test examples 7-15, three samples in each group according to one embodiment The experiment was carried out, and the content of alginic acid in the supernatant was measured after the end of the enzymatic hydrolysis. The alginic acid content in each example was taken as the average value of the alginic acid content measured in each of the three samples. As can be seen from Table 3, Embodiment 11 is a preferred embodiment of each factor.

5The effect of enzymatic hydrolysis time on hydrolysis

Example 16

15 L of fresh seaweed homogenate was taken, added to the enzymatic hydrolysis tank, and then a composite microbial enzyme was added, wherein the composite microbial enzyme was mixed according to the ratio of the preferred embodiment 6, and the reaction temperature and the composite were set according to the process parameters of the preferred example 11. The amount of microbial enzyme added and the pH value were tested and the average alginic acid content was 23.2 g/L.

Fig. 6 shows the effect of the enzymatic hydrolysis time on the enzymatic hydrolysis under the conditions of the preferred embodiment 11, i.e., the enzymatic hydrolysis temperature was 55 ° C, the amount of the composite biological enzyme added was 0.4%, and the pH was 5.5. As the enzymatic hydrolysis time increases, The alginic acid content increased gradually, and the alginic acid content increased linearly when the enzymatic hydrolysis time changed from 0 to 20 h. When the enzymatic hydrolysis time reached 18 h, the alginic acid content reached 20 g/L; when the enzymatic hydrolysis time was about 25 h, the algae The acid content reached a maximum, and the alginic acid content at this time was 23 g/L. Then, as the enzymatic hydrolysis time increases, the alginic acid content tends to balance and no longer changes. For the comprehensive cost consideration, the enzymatic hydrolysis time of the invention is 18h.

6 Preparation of seaweed biological feed

The seaweed biological feed additive or seaweed biological feed of the present invention is prepared after determining the enzyme species of the complex enzyme microbial enzyme and the relevant ratio of various enzymes and the optimal enzymatic process parameters. The method for preparing the seaweed biological feed additive or the seaweed biological feed of the present invention specifically comprises the following steps:

(1) After the seaweed or algae residue is subjected to inorganic impurity removal, the seaweed slurry is cut and ground into particles having a diameter of 1 mm or less; and the seaweed or seaweed residue is ground to less than 1 mm, so that the active nutrients of the seaweed particles are fully exposed, thereby making the composite The microbial enzyme is in full contact with the active material, thereby improving the hydrolysis efficiency.

(2) driving the seaweed slurry into the high-speed dispersion kettle for the first time, adding water and stirring, so that the water and the kelp slurry are thoroughly mixed, and the mass ratio of the first water addition to the kelp slurry is (30-40): 100; It is preferably 35:100.

(3) The mixed seaweed slurry is put into the enzymatic hydrolysis tank, the second time water is added and the pre-formed composite microbial enzyme is added to form a mixed liquid. At this time, the second addition of water is performed, and the ratio of the total amount of the first water addition amount and the second water addition amount to the seaweed slurry is 50:100, and the reasonable control of the water addition amount is advantageous for controlling the moisture content and improving the seaweed feed. Nutritional content.

(4) The mixture is stirred and homogenized, then heat-insulated and enzymatically hydrolyzed, and stirred once every hour during the enzymatic hydrolysis process, and stirred for 5 minutes each time, so that the active nutrients in the seaweed are sufficiently contacted with the complex microbial enzyme to improve hydrolysis. effectiveness.

According to a preferred embodiment, the mixed solution after the above four steps is subjected to solid-liquid separation by a backflushing belt filter, and the separated enzymatic supernatant is concentrated in a range of 60 to 68 ° C; or The enzymatically mixed mixture is dried to obtain the seaweed biological feed additive or seaweed biological feed of the present invention.

According to a preferred embodiment, in the process of stirring, in order to make the seaweed slurry more uniformly mixed, the seaweed pulp and the water can be sufficiently mixed, the rotation speed of the high-speed dispersion kettle after starting is 3000 rpm, and after stirring for 3 minutes, the high-speed dispersion kettle The speed was reduced from 3000 rpm to 1000 rpm.

According to a preferred embodiment, the cellulase, pectinase, protease and amylase of the present invention account for a percentage by mass of the complex microbial enzyme: 60 to 100%, 0 to 40%, 0 to 5%, 0. ~5%, and the cellulase is derived from Trichoderma reesei, Trichoderma viride and/or Aspergillus niger; the pectinase is derived from Rhizopus oryzae, Aspergillus niger and/or Aspergillus oryzae; the protease is derived from papain, bromelain and / or Bacillus subtilis; amylase is derived from Bacillus licheniformis, Aspergillus oryzae and / or Aspergillus niger.

According to a preferred embodiment, the cellulase of the present invention has an enzyme activity of (18-24)×10 ⁴ μg/g, and the pectinase has an enzyme activity of (2-5)×10 ⁴ μg/g. The activity was (10 to 50) × 10 ⁴ μ/g, and the enzyme activity of amylase was (1 to 5) × 10 ⁴ μ/g.

According to a preferred embodiment, the preparation method of the present invention is to add a composite microbial enzyme to the seaweed slurry according to a mass ratio of the composite microbial enzyme to the seaweed slurry of (0.3 to 3):100, and at normal pressure and temperature. The algae biological feed additive or the seaweed biological feed is obtained by insulating the enzyme for 30 to 30 hours under conditions of 30 to 65 ° C and pH 3 to 7.

Under the guidance of the general technical scheme of the present invention, another aspect of the present invention relates to a seaweed biological feed additive or a seaweed biological feed, and the seaweed biological feed additive or the seaweed biological feed of the present invention is prepared according to the preparation method as described above. of.

Moreover, according to a preferred embodiment, the seaweed feed additive or seaweed biological feed of the present invention further comprises one or more of fish meal, fish oil, soybean meal, tapioca starch, corn flour and powdered soybean phospholipid. Moreover, the seaweed biological feed of the present invention can be prepared into a powdery or granular form or an amorphous form as needed.

Under the guidance of the general technical solution of the present invention, another aspect of the present invention relates to a seaweed biological feed, particularly for feeding poultry/livestock or aquaculture. The seaweed biological feed comprises an algae biological feed additive prepared as described above or a seaweed feed additive as described above.

The seaweed biological feed additive or seaweed biological feed prepared by the invention not only greatly improves the digestion and absorption rate of seaweed, reduces waste, but also enhances the immunity of the body and reduces the use of antibiotics, and is a healthy, efficient and novel breeding feed. . Moreover, the invention has simple preparation process, easy operation and short production time as compared with other preparation methods in the prior art, and is suitable for mass production in a short time.

It should be noted that the above specific embodiments are exemplary, and those skilled in the art can devise various solutions inspired by the disclosure of the present invention, and these solutions are also within the scope of the present disclosure and fall into the present disclosure. Within the scope of protection of the invention. It should be understood by those skilled in the art that the claims The scope of protection of the present invention is right The requirements and their equivalents are limited.

Claims

A method for preparing an active seaweed feed additive, characterized in that the method comprises the steps of: adding a microbial enzyme to an algae slurry prepared from seaweed or algae residue to carry out enzymatic hydrolysis to obtain the active seaweed feed additive, wherein The complex microbial enzyme consists of a plurality of cellulases, pectinase, proteases and amylases.
The method for producing an active seaweed feed additive according to claim 1, wherein the quality of said cellulase, said pectinase, said protease and said amylase in said composite microbial enzyme The percentages are: 60 to 100%, 0 to 40%, 0 to 5%, 0 to 5%, and

The cellulase is derived from Trichoderma reesei, Trichoderma viride, and/or Aspergillus niger;

The pectinase is derived from Rhizopus oryzae, Aspergillus niger and/or Aspergillus oryzae;

The protease is derived from papain, bromelain and/or Bacillus subtilis;

The amylase is derived from Bacillus licheniformis, Aspergillus oryzae and/or Aspergillus niger.
The method for producing an active seaweed feed additive according to claim 2, wherein the cellulase has an enzyme activity of (18 to 24) × 10 4 μ/g, and the pectinase has an enzyme activity of (2). ～5)×10 4 μ/g, the protease activity of the protease is (10 to 50)×10 4 μ/g, and the enzyme activity of the amylase is (1 to 5)×10 4 μ/g.
The method for producing an active seaweed feed additive according to claim 1, wherein the method comprises adding a compound to the seaweed slurry according to a mass ratio of the composite microbial enzyme to the seaweed slurry of (0.3 to 3):100. The microbial enzyme is subjected to heat preservation for 6 to 30 hours under normal pressure, temperature of 30 to 65 ° C, and pH 3 to 7, thereby obtaining the active seaweed feed additive.
The method for producing an active seaweed feed additive according to claim 4, wherein the method further comprises the steps of:

The seaweed or algae residue is subjected to inorganic impurity removal, and then ground and ground into an algae slurry having a diameter of about 1 mm;

The seaweed slurry is pumped into the high-speed dispersion kettle for the first time, and the water and the kelp slurry are charged. Mixing, and the mass ratio of the first water addition to the kelp slurry is (30-40): 100;

The mixed seaweed slurry is driven into the enzymatic hydrolysis tank, water is added for the second time, and the composite microbial enzyme is added to form the mixed liquid, and the total amount of the first water addition amount and the second water addition amount and the seaweed The mass ratio of the slurry is 50:100;

The mixture was stirred uniformly, and then heat-insulated and hydrolyzed, and stirred once every hour during the enzymatic hydrolysis, and stirred for 5 minutes each time.
The method for preparing an active seaweed feed additive according to claim 5, wherein the enzymatically mixed mixture is subjected to solid-liquid separation by a backflushing belt filter, and the separated enzymatic hydrolysate is at 60 to 68. Concentration is carried out in the range of °C; or the enzymatically prepared mixture is dried.
The method for preparing an active seaweed feed additive according to claim 5, wherein the rotation speed of the high-speed dispersion vessel after starting is 3000 rpm, and after the agitation for 3 minutes, the rotation speed of the high-speed dispersion kettle is 3000 rpm. Dropped to 1000 rpm.
An active seaweed feed additive, characterized in that the active seaweed feed additive is produced according to the production method according to any one of claims 1 to 7.
The active seaweed feed additive according to claim 8, wherein the active seaweed feed additive further comprises one or more of fish meal, fish oil, soybean meal, tapioca starch, corn flour, and powdered soybean phospholipid.
An active seaweed feed, in particular for feeding poultry/livestock or aquaculture, characterized in that the feed comprises an active seaweed feed additive prepared by the production method according to any one of claims 1 to 7 or The active seaweed feed additive according to claim 8 or 9.