WO2015070746A1

WO2015070746A1 - Test strip card marked by quantum dots

Info

Publication number: WO2015070746A1
Application number: PCT/CN2014/090849
Authority: WO
Inventors: 马义才; 顾敏; 马灵
Original assignee: 成都领御生物技术有限公司; 马义才
Priority date: 2013-11-14
Filing date: 2014-11-11
Publication date: 2015-05-21
Also published as: CN103675267A

Abstract

A test strip card marked by quantum dots, comprising a card case (3) and a quantum dot marked test strip (2); a test strip insertion slot (12) is formed in the opening at one end of the card case (3); the test strip (2) is a detached insertion structure of the card case (3); the card case (3) has a storage medium (13) thereon stored with the standard curve information of a sample object to be tested; when conducting sample testing, one end of the sample absorption pad (4) of a test strip (2) inserted in the card case (3) extends out of the opening end of the card case (3) to dip into and absorb the sample, and an instrument having a signal detection function collects the characteristic signal of a detection band (6) of the test strip (2), and combines the standard curve or coefficient parameter of the object to be tested simultaneously read from the storage medium (13) so as to calculate and obtain the single-component or multicomponent concentration of the sample. The present invention is convenient and fast, and flexible to use, and is highly sensitive and produces objective results.

Description

A quantum dot-labeled test strip card

Technical field

The invention belongs to the field of in-vitro diagnosis, and particularly relates to a quantum dot mark capable of rapidly quantifying a single component or a multi-component concentration of a sample by combining an instrument with a test standard curve or a coefficient parameter and the like, and an instrument having a signal detection function. Test strip card.

Background technique

Gold-Immunochromatography Assay (GICA) has been widely used in all aspects of immunoassay. It uses a microporous membrane as a carrier and uses capillary action of a microporous membrane to make it drop on one end of the membrane strip. The liquid sample slowly migrates to the other end of the microporous membrane. If there is a specific antigen or antibody in the liquid, they can bind to the immunogold and then bind to the corresponding antibody or antigen coated on the microporous membrane to display immunity. Gold color (red). The technique is simple and fast, and the results can be observed with the naked eye in a matter of minutes. The shortcomings are: (1) It is only possible to qualitatively test the sample, and it is difficult to quantify the sample. (2) It is difficult to simultaneously test multiple components of the sample, and the detection efficiency is low. (3) For samples with very low antigen or antibody content, the colloidal gold color is very light and difficult to judge with the naked eye, and the detection sensitivity is low.

It has been a long-term goal for the rapid quantitative detection of multi-component samples. In recent years, nano quantum dots (QDs) have unique and excellent optical properties: high fluorescence efficiency, wide excitation line range, single-element excitation, multiple emission, narrow emission spectrum and symmetry, and slow photobleaching speed. The fluorescence lifetime is long, the particle size is similar to that of biomolecules, and the surface modification can be multi-functionalized. The characteristic wavelength fluorescence spectra produced by the mixture of quantum dots of different particle sizes and kinds do not overlap, which is very suitable for sample multi-component analysis. Sweeny et al. used three primary antibodies with quantum dots of three different emission wavelengths (

605nm and 655nm) form an antibody-quantum dot complex by specific binding of biotin-avidin, and simultaneous analysis of three different antibodies in the tonsil by fluorescence imaging, which proves that the application of multicolor quantum dots can be simultaneously in the same sample. Position multiple biomarkers. Nie et al. used four kinds of different wavelengths of quantum dots to label four kinds of antibodies to achieve imaging detection of Hodgkin's and Reed-Sternberg in Hodgkin's lymphoma, which improved detection sensitivity and specificity. Rapid and accurate diagnosis of Hodgkin's lymphoma. Kobayashi et al. and Chan et al. used two different color near-infrared quantum dot images to analyze the lymphatic flow and left and right thoracic cavity of mice, respectively, demonstrating the multiple imaging analysis of near-infrared quantum dots. In addition to image analysis, qualitative and quantitative analysis of various pathogens, toxins, antigens, antibodies, enzymes, small molecules, and ions can be performed by using the fluorescence characteristics of quantum dots. Zahavy et al. Preparation of fluorescent probes

with

Simultaneous quantitative detection of two pathogenic bacteria was achieved by flow cytometry, and the detection limit was 10 ³ cfu/mL. Goldman's group used multicolor quantum dots to simultaneously detect enterotoxin B and 2,4,6-trinitrotoluene produced by staphylococci and four toxins of cholera toxin, ricin, Shiga toxin and staphylococcal toxin. In addition, quantum dots are used as fluorescent labels in microfluidic protein chips, and their fluorescent pleochroism can simultaneously detect tumor markers carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) with detection limits as low as 250fmol. /L, 4 orders of magnitude higher than detection with organic fluorescent dyes. Giorgio et al. constituting a fluorescent probe with vascular endothelial growth factor A (VEGF A) and angiopoietin (angiopoietin) and quantum dots of different emission wavelengths respectively, and causing aggregation of quantum dots by antigen-antibody specific binding reaction, using flow cytometry The characteristic fluorescent signal was quantitatively detected to obtain the concentration of the corresponding antigen, and the detection limit was 1 pmol/L, and simultaneous detection of various antigens was achieved. In recent years, many researchers have used quantum dot coding to perform multi-component analysis. This technology is mainly encoded by the richness of quantum dot fluorescence color and fluorescence intensity. It can simultaneously measure more components and achieve simultaneous analysis of massive information in protein analysis, DNA analysis and drug screening. . In theory, n kinds of intensity m colors can generate n ^m -1 kinds of codes, and the detection and detection of n ^m -1 kinds of targets can be realized by decoding technology. According to the calculation, only 50,000 to 40,000 identifiable nanoparticle-encoded microspheres can be obtained by combining 5 to 6 colors with 6 quantum dots of different luminescence intensity. If combined with 10 quantum-intensity quantum dot nanoparticles, one million identifiable nanoparticle-encoded microspheres can be obtained. These microspheres can theoretically encode 1 million different DNA or proteins. Simultaneous detection of 1 million different DNAs or proteins in a sample! Nie et al. encapsulated three different color quantum dots of red, green and blue into a polystyrene microsphere at a ratio of 1:1:1, 1:2:1, and 2:1:1 to encode the obtained fluorescence spectrum. Different characteristics enable simultaneous detection of three target DNAs. Using similar techniques, Gu and Sha et al. quantified single-stranded DNA with multiple different sequences by encoding two different wavelengths of quantum dots with three intensities to encode DNA-sensitive hydrogels and microspheres, respectively. High throughput screening typing of multiple oligonucleotides. Wilson and Cooper used this technology to achieve three serum proteins (chicken ovalbumin OVA, bovine serum albumin BSA, human serum albumin HAS) and four immunoglobulin G (human IgG, rabbit IgG, murine IgG, sheep). Simultaneous detection of IgG).

Chinese application number patent 200410010736.3, using colloidal gold labeled antibody to bind to the target, and binding to the quantum dot-labeled antibody on the membrane by immunoreaction to form a detection zone, which is based on the preliminary qualitative or semi-quantitative detection of the detection zone by the naked eye. On the top, the fluorescence quenching effect of the colloidal gold on the fluorescent nanoparticles is utilized, and the detection band is quantitatively detected by detecting the quenching degree of the fluorescent signal. The patent uses two kinds of particle labeling, and the method is not simple enough; the quantification degree of the fluorescent signal is used for quantitative detection, the interference factor is large, and the detection sensitivity is low.

Chinese Patent Application No. 200610024086.7 discloses a method for detecting a quantum dot-labeled rapid immunochromatographic test strip. Although the patent enables multi-component simultaneous detection, it is a method that is claimed, and no request for protection of a quantum dot-labeled rapid immunochromatographic test strip product has been proposed. Moreover, this method has some shortcomings: (1) To achieve multi-component detection of samples, it is necessary to simultaneously set a plurality of corresponding detection lines on the analysis membrane, that is, each detection line is only used to detect one index in the sample, and the detection efficiency is low. If the detector wants to simultaneously detect a large number of components, flux components, and even large components in the sample, it is impossible for a small test strip to achieve the detection target by setting such a large number of corresponding detection lines. (2) Using a UV lamp to illuminate the test strip to observe the presence or absence of the fluorescent line or to determine whether the sample contains the target. In the true sense, this is still a qualitative test, or at most it can only reach a certain The minimum detectable limit of the test object cannot fundamentally achieve accurate concentration detection of any component in the sample, and the sensitivity is still low.

Quantifying the concentration of the test substance using the standard curve of the test subject standard is the main method for quantifying the concentration of the sample in the field of detection. Recently developed storage media in the information field, such as RFID tags (also known as radio frequency identification tags), IC chips, magnetic codes, barcodes, etc., for information access identification is now widely used in computers, communications, electronics, commerce, transportation Control management and other fields, especially RFID tags, small size, large storage information capacity, identification without manual intervention, is an ideal means of information access identification, but few people currently use it for biomedical testing.

The present invention is directed to the deficiencies and shortcomings of the prior art, and provides a quantum dot marking strip card on which a storage medium 13 is mounted, the storage medium 13 storing the same batch of quantum dot marking strips 2 for quantitative detection of the sample. Information for testing such as standard curve or coefficient parameters for concentration. After the sample is subjected to the test strip reaction on the test strip 2, the characteristic signal of the test strip 2 in the test strip 2 is collected by the instrument having the signal detecting function, and the detected object is simultaneously read from the storage medium 13 in combination with the apparatus. The standard curve or coefficient parameters are calculated to obtain the one-component or multi-component concentration of the sample. The invention is characterized in that the sample concentration, in particular the multi-component concentration of the sample, is simple, rapid, sensitive, objective, and flexible.

Summary of the invention

The technical scheme of the present invention is as follows:

The quantum dot-labeled test strip card of the present invention comprises a cartridge 3 and a quantum dot marking strip 2. The quantum dot marking test strip 2 includes a sample pad 4, a marking pad 5, an analysis film 7, and an absorbent pad 8 which are sequentially attached to the substrate 1 by overlapping. The analysis membrane 7 has a detection strip 6. One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 12 to facilitate insertion of the quantum dot marking strip 2 into the cartridge 3. The test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample. The upper casing of the cartridge 3 is provided with a detection window 11 at a position corresponding to the test strip analysis film 7. A storage medium 13 is mounted on the cartridge 3.

The marking pad 5 of the quantum dot marking strip 2 is coated with a single specific molecule related to a target detection of a single quantum dot label, or: a target analyte coated with a label corresponding to a single quantum dot A mixture of related specific molecules is detected. The single quantum dots coated by the marking pad 5 include ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag ₂ S, CdS/PbS, CdS/Cd(OH) ₂ , CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, Any one or any of several kinds of nanoparticles of CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS: Mn, ZnS: Mn, CdS: Cu, ZnS: Cu, CdS: Tb, ZnS: Tb The combination, and the core-shell type nanocomposite particles in which any one of the above quantum dots is a core and silica is a shell.

The detection band 6 of the quantum dot marking strip 2 may be one piece or a plurality of pieces. The test strip 6 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.

The storage medium 13 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode. The storage medium 13 stores the standard curve or coefficient parameter of the sample for quantitative sample concentration of the same batch of quantum dot labeling strip 2, the test strip batch number, the strip validity period, the storage medium password, the clinical index reference value, and the test strip. Manufacturer information, etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.

The standard curve of the test object stored in the storage medium 13 can be selected in various forms, including but not limited to the corresponding relationship between the concentration of the sample standard series and the OD _{detection zone} . The OD _{detection zone is} defined as the detection band optical density value measured by the concentration of the sample standard series.

After the water absorbing pad 8 of the quantum dot marking test strip 2 of the present invention, a test strip reaction end point indicating label 9 can be overlapped. Correspondingly, the test strip reaction end point of the cartridge 3 indicates that the label 9 has a viewing window 11 corresponding thereto. The strip reaction end point indicating label 9 includes a pH test strip having a discoloration range of 5-9.

The marking pad 5 of the quantum dot marking strip 2 of the present invention is a glass fiber membrane; the analysis membrane 7 of the quantum dot marking strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane. The underlayer 1 of the quantum dot marking strip 2 is a polyester or plastic plate; the cartridge 3 is made of polyester, plastic, hard paper material, or other material.

The quantum dot marking test strip 2 of the present invention is a disposable product, and the storage medium 13 on the cartridge 3 and the cartridge 3 is a used product matched with the same batch of quantum dot marking test strips 2.

When the quantum dot marking strip card of the present invention is used for sample detection, the quantum dot marking strip 2 is currently inserted into the cartridge 3, and the interference protruding end of the strip 2 inserted into the cartridge 3 is immersed in the liquid. sample. After the liquid sample is subjected to the test strip reaction on the test strip 2, the characteristic signal of the strip 2 is collected by the instrument having the signal detecting function (such as a reading instrument, a fluorescence detector, etc.) and combined with the instrument to store the strip from the strip. The storage medium 13 simultaneously reads the sample standard curve or coefficient parameter to calculate the single component or multi-component concentration of the sample.

The sample to be tested of the quantum dot-labeled test strip card of the present invention may be from clinical or non-clinical blood, body fluid, urine, saliva, genital tract secretion or other liquid sample or viscous sample, wherein the clinical sample includes Samples of infectious diseases, hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary medicine Samples such as testing, drug testing, etc.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The test strip card combined with the instrument with signal detection function (such as reading instrument, fluorescence detector, etc.) can realize synchronous multi-component rapid quantitative detection (including qualitative detection). Quantum dots of different particle sizes, types and structures can produce continuous fluorescence with different characteristic wavelengths, and the characteristic wavelength fluorescence peak spectra generated by the quantum dot mixture do not overlap. The present invention uses different quantum dots to respectively mark the corresponding reaction molecules of the analyte, and mixes the same. The coating is reacted with the sample to be tested on the test strip 2 of the test strip card, and the characteristic fluorescent signal of the test strip 2 is measured, thereby enabling rapid quantification of the multi-component concentration of the sample.

(2) The time for testing the sample is short, and the result can be obtained in a few minutes. The quantitative sample concentration of the present invention requires the operator to prepare a standard curve of the test object, and the test information such as the standard curve or the coefficient parameter of the test object is stored on the storage medium 13. When the sample is tested, the operator only needs to put the test strip to which the sample is applied to be completed after the test strip reaction, and then put the instrument with the signal detection function, and the instrument collects the characteristic fluorescent signal of the strip 2 of the test strip 2, and combines the The standard test curve or coefficient parameter of the corresponding test object read from the storage medium 13 can quantitatively obtain the sample test result in a few minutes.

(3) The amount of sample is small, and a few microliters to several tens of microliters can meet the detection needs.

(4) The reagents and materials in the test strip are not involved in activity inactivation and can be stored at room temperature for a long time.

DRAWINGS

Figure 1 is a top plan view of a preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a test strip is used as a test line)

Figure 2: The preferred embodiment of the quantum dot-labeled test strip card of the present invention is a side view of the inserted test strip (the test strip uses a test line)

Figure 3 is a top plan view of a preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a plurality of test lines are used for inserting test strips)

Figure 4 is a side view structural view of a preferred embodiment of the quantum dot-labeled test strip card of the present invention, which is a matching test strip (the test strip uses a plurality of test lines)

Figure 5 is a top plan view of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a plug-in test strip adopts a test line)

Figure 6 is a side view structural view of a plug-in test strip of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (the test strip adopts a test line)

Figure 7 is a top plan view of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a plurality of test lines are used for inserting test strips)

Figure 8 is a side view structural view of a plug-in test strip of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (the test strip uses a plurality of test lines)

The serial number is as follows:

1. Underlay, 2, test strip, 3, cartridge, 4, suction pad, 5, marker pad, 6, test tape, 7, analysis membrane, 8, absorbent pad, 9, test strip reaction end indication label, 10, detection window, 11, observation window, 12, test strip slot, 13, storage medium.

detailed description

The following examples and the accompanying drawings are only intended to further illustrate the invention, and those skilled in the art should not limit the scope of the invention.

Embodiment 1

Figures 1-4 illustrate a first embodiment. Embodiment 1 is one of the preferred embodiments of the quantum dot marking strip card of the present invention.

In Figures 1-4, the quantum dot marking strip card of the preferred embodiment comprises a cartridge 3 and a quantum dot marking strip 2. The quantum dot marking test strip 2 includes a sample pad 4, a marking pad 5, an analysis film 7, and an absorbent pad 8 which are sequentially attached to the substrate 1 by overlapping. The analysis membrane 7 has a detection strip 6. One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 12 to facilitate insertion of the quantum dot marking strip 2 into the cartridge 3. The test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample. The upper casing of the cartridge 3 is provided with a detection window 10 at a position corresponding to the test strip analysis film 7. A storage medium 13 is mounted on the cartridge 3.

The marking pad 5 of the quantum dot marking strip 2 is coated with a single specific molecule related to a target detection of a single quantum dot label, or: a target analyte coated with a label corresponding to a single quantum dot A mixture of related specific molecules is detected. The single quantum dot of the test strip 2 marking pad 5 may be selected from the group consisting of ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ ZnS, CdS/Ag ₂ S, CdS/PbS, CdS/Cd(OH) ₂ , CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/ CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, Any one of MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS: Mn, ZnS: Mn, CdS: Cu, ZnS: Cu, CdS: Tb, ZnS: Tb Or a combination of any of several kinds of nanoparticles, and a core-shell type nanocomposite particle in which any one of the above quantum dots is a core and silica is a shell.

The detection strip 6 of the quantum dot marking strip 2 may be one (shown in FIGS. 1 and 2) or a plurality of strips (shown in FIGS. 3 and 4). The test strip 6 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.

The storage medium 13 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode. The standard curve or coefficient parameter of the test object, the test strip batch number, the test strip validity period, the storage medium password, the clinical index reference value, and the test strip manufacturer information are stored in the same batch of quantum dot labeling strip 2 quantitative sample concentration. Etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.

The marking pad 5 of the quantum dot marking strip 2 is a glass fiber membrane; the analysis membrane 7 of the quantum dot marking strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane; The underlayer 1 of the quantum dot marking strip 2 is a polyester or plastic plate; the cartridge 3 is made of polyester, plastic, hard paper material, or other material.

The quantum dot marking strip 2 is a disposable product, and the storage medium 13 on the cartridge 3 and the cartridge 3 is a used product matched with the same batch of quantum dot marking strips 2.

When the quantum dot marking test strip card is used for sample detection, the quantum dot marking test strip 2 is currently inserted into the cartridge 3, and the liquid protruding sample is immersed in the interference protruding end of the test strip 2 inserted into the cartridge 3 to suck the liquid sample. After the liquid sample is subjected to the test strip reaction on the test strip 2, the characteristic signal of the strip 2 is collected by the instrument having the signal detecting function (such as a reading instrument, a fluorescence detector, etc.) and combined with the instrument to store the strip from the strip. The storage medium 13 simultaneously reads the standard curve or coefficient parameter of the test object and calculates the sample. Single or multi-component concentration.

The sample to be tested of the quantum dot-labeled test strip card may be from clinical or non-clinical blood, body fluid, urine, saliva, genital tract secretion or other liquid sample or viscous sample, wherein the clinical sample includes infectious diseases Samples such as hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc. Non-clinical samples include food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, Samples such as drug testing.

Embodiment 2

Figures 5-8 illustrate a second embodiment. Embodiment 2 is another preferred embodiment of the quantum dot marking strip card of the present invention.

In Figures 5-8, the quantum dot marking strip card of the preferred embodiment comprises a cartridge 3 and a quantum dot marking strip 2. The quantum dot marking strip 2 includes a sample pad 4, a marker pad 5, an analysis film 7, an absorbent pad 8 and a test strip reaction end indicating label 9 which are sequentially attached to the substrate 1. The analysis membrane 7 has a detection strip 6. One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 12 to facilitate insertion of the quantum dot marking strip 2 into the cartridge 3. The test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample. The upper casing of the cartridge 3 is provided with a detection window 10 at a position corresponding to the test strip analysis film 7, and the upper casing of the cartridge 3 is provided with an observation window 11 at a position corresponding to the test strip end point indicating label 9, the cartridge A storage medium 13 is mounted on the third.

The marking pad 5 of the quantum dot marking strip 2 is coated with a single specific molecule related to a target detection of a single quantum dot label, or: a target analyte coated with a label corresponding to a single quantum dot A mixture of related specific molecules is detected. The single quantum dot of the strip mark pad 5 may be selected from the group consisting of ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS. , CdS/Ag ₂ S, CdS/PbS, CdS/Cd(OH) ₂ , CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS , CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe Any one of MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS: Mn, ZnS: Mn, CdS: Cu, ZnS: Cu, CdS: Tb, ZnS: Tb or A combination of any of several kinds of nanoparticles, and a core-shell type nanocomposite particle in which any one of the above quantum dots is a core and silica is a shell.

The detection strip 6 of the quantum dot marking strip 2 may be one (shown in FIGS. 5 and 6) or a plurality of strips (shown in FIGS. 7 and 8). The test strip 6 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.

The strip reaction end point indicating label 9 includes a pH test strip having a discoloration range of 5-9. When the sample is tested, the test strip reaction end point label 9 on the test strip can be colored according to the pH condition of the test strip reaction, to indicate whether the test strip reactant has fully oozing through the test strip 6, indicating whether the test strip reaction is sufficient, Whether the test result is valid.

The marking pad 5 of the quantum dot marking strip 2 is a glass fiber membrane; the analysis membrane 7 of the quantum dot marking strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane; the bottom liner 1 of the quantum dot marking strip 2 is a polyester or plastic plate; the cartridge 3 is polyester, plastic, rigid Made of paper materials or other materials.

The quantum dot marking strip 2 is a disposable product. The storage medium 13 on the cartridge 3 and the cartridge 3 is used for the same batch of quantum dot marking strips 2.

When the quantum dot marking test strip card is used for sample detection, the quantum dot marking test strip 2 is currently inserted into the cartridge 3, and the liquid protruding sample is immersed in the interference protruding end of the test strip 2 inserted into the cartridge 3 to suck the liquid sample. Test strip reaction end point indication label 9 color display indicator After the strip test is completed on the test strip 2, the characteristic signal of the test strip 2 is detected by the instrument having the signal detection function (such as a reading instrument, a fluorescence detector, etc.) The single component or multi-component concentration of the sample is calculated by combining the standard curve or coefficient parameter of the sample simultaneously read from the test strip storage medium 13 by the instrument.

It should be noted that the quantum dot-labeled test strip card of the present invention may have other improvements, such as a storage medium 13 storing detection information such as a test sample standard curve, in addition to the cartridge 3 mounted on the test strip card. Above and outside, it can also be directly mounted on the quantum dot marking strip 2; based on the same principle of the invention, the quantum dot marking strip inserted into the cartridge 3 can also be extended to replace the quantum dot labeled biochip (including antigenic antibodies). Chips, protein chips, nucleic acid chips, microfluidic chips, etc. Therefore, any other technical solution formed by any equivalent replacement or equivalent transformation of the quantum dot-labeled test strip card of the present invention falls within the scope of protection of the present invention.

Claims

A quantum dot-labeled test strip card comprising a cartridge (3) and a quantum dot marking strip (2), the quantum dot marking strip (2) comprising a sample attached to the substrate (1) in a sequential overlap Pad (4), marking pad (5), analysis film (7), absorbent pad (8), analysis film (7) has detection tape (6), detection tape (6) is one or more, and the cartridge (3) The upper box surface has a detection window (10) at a position corresponding to the strip analysis film (7), which is characterized by:

One end of the card box (3) and the upper box surface opening of the card box end form a test strip slot (12), and the test strip (2) is a separate plug-in structure of the card box (3), when the sample is tested, a test strip (2) inserted into the cartridge (3), one end of the suction pad (4) is over-extended to extend out of the open end of the cartridge (3) to dip the sample;

The storage box (3) is mounted with a storage medium (13);

The marking pad (5) of the quantum dot marking strip (2) is coated with a single quantum dot-labeled target specific analyte for detecting a single specific molecule, or: each coated with a different single quantum dot corresponding label Target analytes detect a mixture of related specific molecules;

The detection band (6) of the quantum dot labeling strip (2) is coated with another specific molecule associated with detection of a target analyte, or: another specific molecule coated with detection of each target analyte mixture.
The quantum dot-labeled test strip card according to claim 1, wherein said single quantum dot of said marking pad (5) of said quantum dot marking strip (2) comprises ZnS, CdS, HgS, ZnSe , CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag 2 S, CdS/PbS, CdS/Cd(0H) 2 , CdS/HgS, CdS /HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe /CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS: Mn , ZnS: Mn, CdS: Cu, ZnS: Cu, CdS: Tb, ZnS: Tb, or a combination of any of several kinds of nanoparticles, and any one of the above quantum dots is a core, and silica is a shell Core-shell nanocomposite particles.
The quantum dot-labeled test strip card of claim 1 wherein said storage medium (13) comprises an RFID tag, an IC chip, a magnetic code, or a barcode.
The quantum dot-labeled test strip card according to claim 1, wherein the storage medium (13) stores the same batch of quantum dot-labeled test strips (2) the standard curve of the sample for quantitative sample concentration Or coefficient parameter, test strip batch number, test strip validity period, storage medium password, clinical index reference value, test strip manufacturer information, and can read the object identification information, detector information, sample name, sample number, detection Date, test result information.
The quantum dot-labeled test strip card according to claim 4, wherein the standard curve of the test object stored in the storage medium (13) has various forms to be selected, and includes a standard sample series of the test object. A correspondence curve between the concentration and the OD detection zone ; the OD detection zone is defined as a detection band optical density value measured by the concentration of the sample standard series.
The quantum dot-labeled test strip card according to claim 1, wherein:

The marking pad (5) of the quantum dot marking strip (2) is a glass fiber membrane, and the analysis membrane (7) of the quantum dot marking strip (2) is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose / cellulose acetate mixed film;

The underlayer (1) of the quantum dot marking strip (2) is a polyester or plastic plate;

The cartridge (3) is made of polyester, plastic, or a hard paper material.
The quantum dot-labeled test strip card according to claim 1, wherein said quantum dot marking test strip (2) is a disposable product, said cartridge (3) and said cartridge (3) The storage medium (13) is used for the same batch of quantum dot marking strips (2).
The quantum dot-labeled test strip card according to claim 1, wherein when the sample is detected, the quantum dot marking test strip (2) is currently inserted into the cartridge (3) and inserted into the cartridge (3). The interference protruding end of the test strip (2) is immersed to suck the sample, and after the sample is reacted on the test strip (2), the test strip is collected by the instrument with the signal detection function (3) the inner test strip (2) the test strip The characteristic signal of (6) is calculated in conjunction with the standard curve or coefficient parameter of the test object simultaneously read by the instrument from the storage medium (13) to obtain a single component or multi-component concentration of the sample.
A quantum dot-labeled test strip card according to claim 1, wherein the water-absorbing pad (8) of the quantum dot-labeled test strip (2) is further overlapped with a test strip reaction end point indicating label ( 9); the test strip of the cartridge (3) reflects the end point indication label (9) with an observation window (11) correspondingly.
The quantum dot-labeled test strip card according to claim 9, wherein the test strip reaction end point indicating label (9) comprises a pH test strip having a discoloration range of 5-9.
The quantum dot-labeled test strip card according to claim 9, wherein said single quantum dot of said marking pad (5) of said quantum dot marking strip (2) comprises ZnS, CdS, HgS, ZnSe , CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag 2 S, CdS/PbS, CdS/Cd(0H) 2 , CdS/HgS, CdS /HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe /CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS: Mn , ZnS: Mn, CdS: Cu, ZnS: Cu, CdS: Tb, ZnS: Tb, or a combination of any of several kinds of nanoparticles, and any one of the above quantum dots is a core, and silica is a shell Core-shell nanocomposite particles.
A quantum dot-labeled test strip card according to claim 9, wherein said storage medium (13) comprises an RFID tag, an IC chip, a magnetic code, or a barcode.
The quantum dot-labeled test strip according to claim 9, wherein the storage medium (13) stores the same batch of quantum dot-labeled test strips (2) the standard curve of the sample for quantitative sample concentration Or coefficient parameter, test strip batch number, test strip validity period, storage medium password, clinical index reference value, test strip manufacturer information, and can read the object identification information, detector information, sample name, sample number, detection Date, test result information.
The quantum dot-labeled test strip card according to claim 13, characterized in that the standard curve of the test object stored in the storage medium (13) has various forms to be selected, which includes the standard sample series of the test object. A correspondence curve between the concentration and the OD detection zone ; the OD detection zone is defined as a detection band optical density value measured by the concentration of the sample standard series.
The quantum dot-labeled test strip card according to claim 9, wherein:

The marking pad (5) of the quantum dot marking strip (2) is a glass fiber membrane, and the analysis membrane (7) of the quantum dot marking strip (2) is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose / cellulose acetate mixed film;

The underlayer (1) of the quantum dot marking strip (2) is a polyester or plastic plate;

The cartridge (3) is made of polyester, plastic, or a hard paper material.
The quantum dot-labeled test strip card according to claim 9, wherein said quantum dot marking test strip (2) is a disposable product, said cartridge (3) and said cartridge (3) The storage medium (13) is used for the same batch of quantum dot marking strips (2).
The quantum dot-labeled test strip card according to claim 9, wherein when the sample is detected, the quantum dot marking test strip (2) is currently inserted into the cartridge (3) and inserted into the cartridge (3). The interference protruding end of the test strip (2) is immersed to suck the sample, and the test strip reaction end indication label (9) indicates that after the sample is reacted on the test strip (2), the cassette is collected by the instrument having the signal detecting function ( 3) The inner test strip (2) detects the characteristic signal of the strip (6) and calculates the one-component or multi-component of the sample by combining the standard curve or coefficient parameter of the test object read simultaneously from the storage medium (13). concentration.
A quantum dot-labeled test strip according to claim 1 or 9, wherein the sample to be tested is from clinical or non-clinical blood, body fluid, urine, saliva, genital secretions or other liquid samples. Or viscous samples, including clinical samples including infectious diseases, hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, Samples for bio-contamination testing, biologics testing, veterinary testing, and drug testing.