WO2014050021A1 - Centrifugation container - Google Patents

Centrifugation container Download PDF

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Publication number
WO2014050021A1
WO2014050021A1 PCT/JP2013/005498 JP2013005498W WO2014050021A1 WO 2014050021 A1 WO2014050021 A1 WO 2014050021A1 JP 2013005498 W JP2013005498 W JP 2013005498W WO 2014050021 A1 WO2014050021 A1 WO 2014050021A1
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Prior art keywords
container
centrifugation
opening
centrifuge
sample
Prior art date
Application number
PCT/JP2013/005498
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French (fr)
Japanese (ja)
Inventor
朋宣 西尾
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富士フイルム株式会社
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Publication of WO2014050021A1 publication Critical patent/WO2014050021A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • B04B1/02—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles without inserted separating walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/048—Function or devices integrated in the closure enabling gas exchange, e.g. vents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs

Definitions

  • the present invention relates to a centrifuge container for centrifuging a sample component in a container by being rotated about a central axis of the container.
  • This centrifuge device stores a specimen such as blood inside a centrifuge container, and centrifuges the components of the specimen in the container by rotating the centrifuge container around the central axis as a rotation axis. .
  • the centrifuge container used in the above-described centrifuge includes a reservoir that stores the specimen inside the container body, and an opening for injecting the specimen into the reservoir is provided in the container body. It is inclined so as to become higher from the center toward the outer periphery, and has an annular trap portion having a volume for storing a centrifugally separated component having a high specific gravity.
  • a separation gel having an intermediate specific gravity between the components to be separated in the specimen is arranged, and when the container is rotated while the specimen is injected into the storage part, only the component having a higher specific gravity than the separation gel is provided. Moves to the outer peripheral side of the separation gel in the trap portion, and the component having a specific gravity lower than that of the separation gel remains inside the separation gel, whereby the components of the specimen can be separated.
  • the sample in the reservoir may be aerosolized and scattered from the opening to the outside.
  • the present invention has been made in view of the above problems, and in a centrifuge container for centrifuging components of a specimen in a container by being rotated about the central axis of the container, the specimen is centrifuged at the time of centrifugation.
  • Centrifuge that can automatically dispense extracted components from a centrifuge container after centrifuging without using a device that automatically opens the lid and prevents the aerosol from being aerosolized.
  • the object is to provide a separation container.
  • the centrifuge container of the present invention includes a reservoir for storing the specimen inside the container, and an opening for injecting the specimen into the reservoir is provided in the container, and the container is rotated about the central axis of the container as a rotation axis.
  • a centrifuge container for centrifuging the components of the specimen in the reservoir further comprising an attachable sealing lid that closes the opening, the sealing lid being attached to the opening by a dispensing tip. It has a sealing member that can be penetrated and that can collect a sample in the reservoir by a penetrating dispensing tip.
  • the opening is preferably on the central axis of the container.
  • the sealing member may be a flexible sheet in which a septum or a pinhole is formed.
  • the centrifuge container of the present invention further includes a sealable lid that closes the opening and that can be attached.
  • a sealable lid that closes the opening and that can be attached.
  • the sealable lid When the sealable lid is attached to the opening, it can be penetrated by the dispensing tip, and dispensed through. Since the chip has a seal member that can collect the specimen in the reservoir, it can prevent the specimen from being aerosolized and scattered outside, and automatically extract components from the centrifuge container after centrifugation.
  • the centrifuge container of the present invention if the opening is arranged on the central axis of the container, the centrifugal force applied to the sealing member of the sealing lid at the time of centrifugation is reduced. The risk of damage is reduced. Further, if the strength of the seal member is lowered, the seal member can be penetrated by a dispensing tip with a small pressing force, and therefore the operation during automatic dispensing can be facilitated.
  • the surface of the seal member is arranged so as to extend in a direction substantially perpendicular to the central axis of the container, no shear force is applied to the seal member during centrifugation, and only a tensile force in the outer peripheral direction is applied. Similarly to the above, even if the strength of the seal member is lowered, the possibility that the seal member is damaged is lowered.
  • the sealing lid can be reused.
  • FIG. 1 The top view of the container for centrifugation of one embodiment of the present invention
  • Sectional view of the above centrifuge container (sectional view taken along line II-II in FIG. 1) Schematic configuration diagram of a centrifuge using the above centrifuge container
  • the figure which shows the state at the time of centrifugation in the said container for centrifugation The figure which shows the state after centrifugation in the said container for centrifugation
  • FIG. 1 is a top view of a centrifuge container according to an embodiment of the present invention
  • FIG. 2 is a cross-sectional view of the centrifuge container (a cross-sectional view taken along the line II-II in FIG. 1).
  • the centrifuge container 1 includes a storage unit 11 that stores a specimen inside a container body 10 that is substantially cylindrical in appearance, and injects the specimen into the storage unit 11 on the upper surface of the container body 10.
  • the opening 12 is provided.
  • the container body 10 is formed by fitting and fixing the upper member 10a and the lower member 10b, and the lower surface of the storage portion 11 formed between the upper member 10a and the lower member 10b is the outer periphery from the center.
  • An annular trap portion 13 having a volume for storing a high specific gravity component centrifuged from the specimen is formed on the outer peripheral portion of the storage portion 11.
  • a separation gel 14 having an intermediate specific gravity between components to be separated in the specimen is disposed.
  • a gel having a specific gravity intermediate between the blood cell component and the plasma component may be used as the separation gel 14.
  • a detachable sealing lid 20 that closes the opening is attached to the opening 12.
  • the sealing lid 20 has a chip insertion port 21 that communicates with the storage part 11 of the container body 10 and the outside when mounted on the opening 12.
  • the tip insertion port 21 is provided with a seal member 22 that can be penetrated by a dispensing tip.
  • the chip insertion port 21 is blocked by the seal member 22, and the storage portion 11 is sealed when the sealing lid 20 is attached to the opening 12.
  • the seal member 22 may be provided on either the upper side or the lower side of the chip insertion port 21. However, the seal member 22 is provided on the lower side of the chip insertion port 21 when the user attaches the sealing lid 20 to the opening 12. Since the user's finger is less likely to touch the seal member 22, it is possible to prevent the seal member 22 from being accidentally damaged.
  • the opening 12 is disposed on the central axis C of the container body 10.
  • the centrifugal force applied to the seal member 22 of the sealing lid 20 at the time of centrifugation is reduced, so that the risk of damage to the seal member 22 is reduced even if the strength of the seal member 22 is reduced.
  • the surface of the seal member 22 is arranged so as to extend in a direction substantially orthogonal to the central axis of the centrifuge container, whereby no shear force is applied to the seal member 22 during centrifugal separation, and a tensile force in the outer circumferential direction.
  • the seal member 22 can be penetrated by a dispensing tip with a small pressing force, and thus the operation during automatic dispensing can be facilitated.
  • FIG. 3 is a schematic configuration diagram of a centrifuge using the centrifuge container.
  • the centrifuge 50 includes a housing 52 for housing the centrifuge container 1 inside the housing 51.
  • the accommodating portion 52 is provided with a holding portion 53 for holding the centrifuge container 1, and this holding portion 53 is rotatably supported by a rotation mechanism (not shown), and the centrifuge held by the holding portion 53.
  • the center axis C of the separation container 1 is configured to coincide with the rotation axis of the rotation mechanism.
  • FIG. 4 is a diagram showing a state of the centrifuge container when the sample is charged
  • FIG. 5 is a diagram showing a centrifuge state of the centrifuge container
  • FIG. 6 is a centrifuge state of the centrifuge container.
  • FIG. Here, a case where blood is used as a sample is shown as an example.
  • blood 30 is injected into the storage part 11 of the centrifuge container 1. Specifically, blood 30 is injected from the opening 12 provided on the upper surface of the container body 10 by a pipette, a syringe or the like. After the blood 30 is injected, the sealing lid 20 is attached to the opening 12.
  • the centrifuge container 1 into which the blood 30 has been injected is accommodated in the accommodating part 52 of the centrifuge 50 and rotated about the central axis C of the centrifuge container 1 as a rotation axis. Then, as shown in FIG. 5, only the blood cell component 31 having a specific gravity higher than that of the separation gel 14 moves to the outer peripheral side of the separation gel 14 in the trap portion 13, and the plasma component having a specific gravity lower than that of the separation gel 14. 32 sticks to the inner peripheral side of the separation gel 14.
  • the blood 30 in the reservoir 11 may be aerosolized and scattered from the opening 12 to the outside.
  • the sealing lid 20 since the sealing lid 20 is attached to the opening 12, the aerosolized blood It is possible to prevent 30 from being scattered outside the container.
  • the blood cell component 31 on the outer peripheral side of the separation gel 14 remains trapped in the trap part 13 and is more internal than the separation gel 14. Only the plasma component 32 on the circumferential side descends and is stored near the center of the lower surface of the storage unit 11. Thereby, only the plasma component 32 can be extracted.
  • the plasma component 32 when the plasma component 32 is automatically dispensed from the centrifuge container 1 after centrifugation, it is not necessary to remove the sealing lid 20, and the dispensing tip 40 is directly put into the chip insertion port 21 of the sealing lid 20. Since the plasma component 32 in the reservoir 11 can be dispensed by being inserted and penetrating the seal member 22, the plasma component 32 can be automatically dispensed without using a device that automatically opens the lid. Can correspond to dispensing.
  • the sealing member is not a sheet-like member that cannot be repaired once penetrated as described above, but the septum or pinhole is formed in the flexible sheet, the sealing lid is re-opened. It can be used.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)

Abstract

[Problem] A centrifugation container for the centrifugation of components of a sample in a container by rotating with the central axis of the container as the axis of rotation, wherein the sample is prevented from being aerosolized during centrifugation and being scattered outside, and which supports automatic dispensing of the extraction component from the centrifugation container after centrifugation without using the likes of a device that automatically opens the lid. [Solution] A centrifugation container (1) which is equipped with a storage part (11) for storing a sample in a container main body (10) having a substantially cylindrical appearance, and in which the upper surface of the container main body (10) is provided with an opening (12) for pouring the sample into the storage part (11). The centrifugation container (1) is further provided with an attachable sealing lid (20) that blocks the opening (12). The sealing lid (20) has a seal member (22) that, when the sealing lid (20) is attached to the opening (12), can be penetrated with a dispensing tip, and enables the sample inside the storage part (11) to be extracted by the dispensing tip that has penetrated the seal member (22).

Description

遠心分離用容器Centrifuge container
　本発明は、容器の中心軸を回転軸として回転させられることにより容器内の検体の成分を遠心分離するための遠心分離用容器に関する。 The present invention relates to a centrifuge container for centrifuging a sample component in a container by being rotated about a central axis of the container.
　従来より、血液等の検体の成分を遠心分離するための遠心分離装置として特許文献１、２のような装置が知られている。 Conventionally, devices as disclosed in Patent Documents 1 and 2 are known as centrifuges for centrifuging a sample component such as blood.
　この遠心分離装置は、遠心分離用容器の内部に血液等の検体を貯留し、遠心分離用容器の中心軸を回転軸として回転させることにより、容器内の検体の成分を遠心分離するものである。 This centrifuge device stores a specimen such as blood inside a centrifuge container, and centrifuges the components of the specimen in the container by rotating the centrifuge container around the central axis as a rotation axis. .
　上記の遠心分離装置に用いられる遠心分離用容器は、容器本体内部に検体を貯留する貯留部を備えるとともに、容器本体に検体を貯留部内に注入するための開口が設けられ、貯留部の下面は中心から外周に向けて高くなるように傾斜しており、外周部には遠心分離した高比重の成分を収納する容積を持った環状のトラップ部を有する。 The centrifuge container used in the above-described centrifuge includes a reservoir that stores the specimen inside the container body, and an opening for injecting the specimen into the reservoir is provided in the container body. It is inclined so as to become higher from the center toward the outer periphery, and has an annular trap portion having a volume for storing a centrifugally separated component having a high specific gravity.
　トラップ部には検体において分離したい成分同士の中間の比重を有する分離用ゲルが配されており、貯留部内に検体を注入した状態で容器を回転させると、分離用ゲルよりも高い比重の成分のみがトラップ部において分離用ゲルよりも外周側に移動し、分離用ゲルよりも低い比重の成分は分離用ゲルよりも内側に留まるため、これにより検体の成分を分離することができる。 In the trap part, a separation gel having an intermediate specific gravity between the components to be separated in the specimen is arranged, and when the container is rotated while the specimen is injected into the storage part, only the component having a higher specific gravity than the separation gel is provided. Moves to the outer peripheral side of the separation gel in the trap portion, and the component having a specific gravity lower than that of the separation gel remains inside the separation gel, whereby the components of the specimen can be separated.
米国特許出願公開第２００７／００７７１８３号明細書US Patent Application Publication No. 2007/0077183 国際公開第２００８／１４０７４２号International Publication No. 2008/140742 特開平７－７７５２９号公報JP 7-77529 A 特開２００７－２３２３９９号公報JP 2007-232399 A
　上記のような遠心分離用容器を回転させて遠心分離を行うと、貯留部内の検体がエアロゾル化して開口から外部に飛散するおそれがある。このように検体がエアロゾル化して外部に飛散するのを防止するために、特許文献３、４に記載のように、容器に蓋をすることが考えられる。 If the centrifuge is rotated by rotating the centrifuge container as described above, the sample in the reservoir may be aerosolized and scattered from the opening to the outside. Thus, in order to prevent the specimen from being aerosolized and scattered outside, it is conceivable to cover the container as described in Patent Documents 3 and 4.
　しかしながら、遠心分離用容器の開口に蓋を設けてしまうと、遠心分離後の遠心分離用容器から抽出成分を自動的に分注しようとしたときに、特許文献３、４に記載されているように蓋を自動的に開栓する装置が必要となるため、装置が大掛かりとなり、またコストも増加してしまう。 However, if a lid is provided at the opening of the centrifuge container, it is described in Patent Documents 3 and 4 when an extraction component is automatically dispensed from the centrifuge container after centrifugation. Since a device for automatically opening the lid is required, the device becomes large and the cost also increases.
　本発明は、上記問題に鑑みてなされたものであり、容器の中心軸を回転軸として回転させられることにより容器内の検体の成分を遠心分離するための遠心分離用容器において、遠心分離時に検体がエアロゾル化して外部に飛散するのを防止するとともに、蓋を自動的に開栓するような装置を用いることなく、遠心分離後の遠心分離用容器からの抽出成分の自動分注に対応した遠心分離用容器を提供することを目的とするものである。 The present invention has been made in view of the above problems, and in a centrifuge container for centrifuging components of a specimen in a container by being rotated about the central axis of the container, the specimen is centrifuged at the time of centrifugation. Centrifuge that can automatically dispense extracted components from a centrifuge container after centrifuging without using a device that automatically opens the lid and prevents the aerosol from being aerosolized. The object is to provide a separation container.
　本発明の遠心分離用容器は、容器内部に検体を貯留する貯留部を備えるとともに、容器に検体を貯留部内に注入するための開口が設けられ、容器の中心軸を回転軸として回転させられることにより貯留部内の検体の成分を遠心分離するための遠心分離用容器であって、開口を塞ぐ、装着可能な密閉蓋をさらに備え、密閉蓋は、開口に装着された際に、分注チップにより貫通可能であり、貫通した分注チップにより貯留部内の検体を採取可能とするシール部材を有することを特徴とする。 The centrifuge container of the present invention includes a reservoir for storing the specimen inside the container, and an opening for injecting the specimen into the reservoir is provided in the container, and the container is rotated about the central axis of the container as a rotation axis. A centrifuge container for centrifuging the components of the specimen in the reservoir, further comprising an attachable sealing lid that closes the opening, the sealing lid being attached to the opening by a dispensing tip. It has a sealing member that can be penetrated and that can collect a sample in the reservoir by a penetrating dispensing tip.
　本発明の遠心分離用容器において、開口は、容器の中心軸上にあることが好ましい。 In the centrifuge container of the present invention, the opening is preferably on the central axis of the container.
　また、シール部材は、可撓性シートにセプタムまたはピンホールが形成されたものとしてもよい。 Further, the sealing member may be a flexible sheet in which a septum or a pinhole is formed.
　本発明の遠心分離用容器によれば、開口を塞ぐ、装着可能な密閉蓋をさらに備え、この密閉蓋について、開口に装着された際に、分注チップにより貫通可能であり、貫通した分注チップにより貯留部内の検体を採取可能とするシール部材を有するものとしたので、検体がエアロゾル化して外部に飛散するのを防止できるとともに、遠心分離後の遠心分離用容器から抽出成分を自動的に分注する際に密閉蓋を外す必要がなく、分注チップをそのまま密閉蓋のシール部材に貫通させて分注を行うことができるので、蓋を自動的に開栓するような装置を用いることなく、抽出成分の自動分注に対応させることができる。 The centrifuge container of the present invention further includes a sealable lid that closes the opening and that can be attached. When the sealable lid is attached to the opening, it can be penetrated by the dispensing tip, and dispensed through. Since the chip has a seal member that can collect the specimen in the reservoir, it can prevent the specimen from being aerosolized and scattered outside, and automatically extract components from the centrifuge container after centrifugation. There is no need to remove the sealing lid when dispensing, and dispensing can be performed by directly passing the dispensing tip through the sealing member of the sealing lid, so use a device that automatically opens the lid. And can be adapted to automatic dispensing of extracted components.
　本発明の遠心分離用容器において、開口を、容器の中心軸上に配置すれば、遠心分離時に密閉蓋のシール部材にかかる遠心力が小さくなるため、シール部材の強度を下げてもシール部材が破損するおそれが低下する。また、シール部材の強度を下げれば、小さい押圧力により分注チップでシール部材を貫通させることができるため、自動分注時の動作を容易にすることができる。 In the centrifuge container of the present invention, if the opening is arranged on the central axis of the container, the centrifugal force applied to the sealing member of the sealing lid at the time of centrifugation is reduced. The risk of damage is reduced. Further, if the strength of the seal member is lowered, the seal member can be penetrated by a dispensing tip with a small pressing force, and therefore the operation during automatic dispensing can be facilitated.
　なお、シール部材の面を容器の中心軸と略直交する方向に延びるように配すれば、遠心分離時にシール部材にせん断力はかからず、外周方向への引っ張り力のみが掛かるようになるので、上記と同様に、シール部材の強度を下げてもシール部材が破損するおそれが低下する。 If the surface of the seal member is arranged so as to extend in a direction substantially perpendicular to the central axis of the container, no shear force is applied to the seal member during centrifugation, and only a tensile force in the outer peripheral direction is applied. Similarly to the above, even if the strength of the seal member is lowered, the possibility that the seal member is damaged is lowered.
　また、シール部材を、可撓性シートにセプタムまたはピンホールが形成されたものとすれば、密閉蓋を再利用することが可能となる。 Also, if the sealing member is a flexible sheet having a septum or pinhole, the sealing lid can be reused.
本発明の一実施の形態の遠心分離用容器の上面図The top view of the container for centrifugation of one embodiment of the present invention 上記遠心分離用容器の断面図（図１のII－II線断面図）Sectional view of the above centrifuge container (sectional view taken along line II-II in FIG. 1) 上記遠心分離用容器を用いた遠心分離装置の概略構成図Schematic configuration diagram of a centrifuge using the above centrifuge container 上記遠心分離用容器における検体投入時の状態を示す図The figure which shows the state at the time of the sample injection | throwing-in in the said container for centrifugation. 上記遠心分離用容器における遠心分離時の状態を示す図The figure which shows the state at the time of centrifugation in the said container for centrifugation 上記遠心分離用容器における遠心分離後の状態を示す図The figure which shows the state after centrifugation in the said container for centrifugation
　以下、図面を参照して本発明の実施の形態について詳細に説明する。図１は本発明の一実施の形態の遠心分離用容器の上面図、図２は上記遠心分離用容器の断面図（図１のII－II線断面図）である。 Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. FIG. 1 is a top view of a centrifuge container according to an embodiment of the present invention, and FIG. 2 is a cross-sectional view of the centrifuge container (a cross-sectional view taken along the line II-II in FIG. 1).
　本実施の形態の遠心分離用容器１は、外観が略円柱形状の容器本体１０内部に検体を貯留する貯留部１１を備えるとともに、容器本体１０の上面に検体を貯留部１１内に注入するための開口１２が設けられたものである。 The centrifuge container 1 according to the present embodiment includes a storage unit 11 that stores a specimen inside a container body 10 that is substantially cylindrical in appearance, and injects the specimen into the storage unit 11 on the upper surface of the container body 10. The opening 12 is provided.
　容器本体１０は、上側部材１０ａと下側部材１０ｂとを嵌合させて固着したものであり、上側部材１０ａと下側部材１０ｂとの間に形成される貯留部１１の下面は、中心から外周に向けて高くなるように傾斜しており、また貯留部１１の外周部には検体から遠心分離された高比重の成分を収納する容積を持った環状のトラップ部１３が形成されている。トラップ部１３には、検体において分離したい成分同士の中間の比重を有する分離用ゲル１４が配されている。例えば、検体として血液を用いて、血液を血球成分と血漿成分に分離する場合には、分離用ゲル１４としては血球成分と血漿成分の中間の比重を有するものを用いればよい。 The container body 10 is formed by fitting and fixing the upper member 10a and the lower member 10b, and the lower surface of the storage portion 11 formed between the upper member 10a and the lower member 10b is the outer periphery from the center. An annular trap portion 13 having a volume for storing a high specific gravity component centrifuged from the specimen is formed on the outer peripheral portion of the storage portion 11. In the trap portion 13, a separation gel 14 having an intermediate specific gravity between components to be separated in the specimen is disposed. For example, when blood is used as a specimen and blood is separated into a blood cell component and a plasma component, a gel having a specific gravity intermediate between the blood cell component and the plasma component may be used as the separation gel 14.
　また、図２に示すように、開口１２には、開口を塞ぐ、着脱可能な密閉蓋２０が取り付けられる。この密閉蓋２０は、開口１２に装着された際に容器本体１０の貯留部１１と外部を連通するチップ挿入口２１を有する。チップ挿入口２１には分注チップにより貫通可能なシール部材２２が配されている。このシール部材２２によりチップ挿入口２１は遮断されており、密閉蓋２０が開口１２に装着された際に貯留部１１は密閉される。なお、シール部材２２はチップ挿入口２１の上側もしくは下側のいずれに設けてもよいが、チップ挿入口２１の下側に設けた方が、ユーザーが密閉蓋２０を開口１２に取り付ける際に、ユーザーの指がシール部材２２に触れることが少なくなるので、誤ってシール部材２２が破損するのを防止することができる。 Further, as shown in FIG. 2, a detachable sealing lid 20 that closes the opening is attached to the opening 12. The sealing lid 20 has a chip insertion port 21 that communicates with the storage part 11 of the container body 10 and the outside when mounted on the opening 12. The tip insertion port 21 is provided with a seal member 22 that can be penetrated by a dispensing tip. The chip insertion port 21 is blocked by the seal member 22, and the storage portion 11 is sealed when the sealing lid 20 is attached to the opening 12. The seal member 22 may be provided on either the upper side or the lower side of the chip insertion port 21. However, the seal member 22 is provided on the lower side of the chip insertion port 21 when the user attaches the sealing lid 20 to the opening 12. Since the user's finger is less likely to touch the seal member 22, it is possible to prevent the seal member 22 from being accidentally damaged.
　また、本実施の形態の遠心分離用容器１では、開口１２が、容器本体１０の中心軸Ｃ上に配置されている。これにより、遠心分離時に密閉蓋２０のシール部材２２にかかる遠心力が小さくなるため、シール部材２２の強度を下げてもシール部材２２が破損するおそれが低下する。さらに、シール部材２２の面は遠心容器の中心軸と略直交する方向に延びるように配されており、これにより、遠心分離時にシール部材２２にせん断力はかからず、外周方向への引っ張り力のみが掛かるようになるので、上記と同様に、シール部材２２の強度を下げてもシール部材が破損するおそれが低下する。このようにシール部材２２の強度を下げれば、小さい押圧力により分注チップでシール部材２２を貫通させることができるため、自動分注時の動作を容易にすることができる。 Further, in the centrifuge container 1 of the present embodiment, the opening 12 is disposed on the central axis C of the container body 10. As a result, the centrifugal force applied to the seal member 22 of the sealing lid 20 at the time of centrifugation is reduced, so that the risk of damage to the seal member 22 is reduced even if the strength of the seal member 22 is reduced. Further, the surface of the seal member 22 is arranged so as to extend in a direction substantially orthogonal to the central axis of the centrifuge container, whereby no shear force is applied to the seal member 22 during centrifugal separation, and a tensile force in the outer circumferential direction. As described above, even if the strength of the seal member 22 is lowered, the possibility that the seal member is damaged is reduced. If the strength of the seal member 22 is lowered in this way, the seal member 22 can be penetrated by a dispensing tip with a small pressing force, and thus the operation during automatic dispensing can be facilitated.
　次に、上記遠心分離用容器を用いた遠心分離装置について説明する。図３は上記遠心分離用容器を用いた遠心分離装置の概略構成図である。 Next, a centrifuge using the centrifuge container will be described. FIG. 3 is a schematic configuration diagram of a centrifuge using the centrifuge container.
　この遠心分離装置５０は、筐体５１内部に遠心分離用容器１を収容する収容部５２を備える。収容部５２には遠心分離用容器１を保持する保持部５３が設けられており、この保持部５３は不図示の回転機構により回転自在に支持されており、保持部５３で保持している遠心分離用容器１の中心軸Ｃが回転機構の回転軸と一致するように構成されている。 The centrifuge 50 includes a housing 52 for housing the centrifuge container 1 inside the housing 51. The accommodating portion 52 is provided with a holding portion 53 for holding the centrifuge container 1, and this holding portion 53 is rotatably supported by a rotation mechanism (not shown), and the centrifuge held by the holding portion 53. The center axis C of the separation container 1 is configured to coincide with the rotation axis of the rotation mechanism.
　次に、上記遠心分離装置において遠心分離用容器内の検体を遠心分離する際の作用効果について説明する。図４は上記遠心分離用容器における検体投入時の状態を示す図、図５は上記遠心分離用容器における遠心分離時の状態を示す図、図６は上記遠心分離用容器における遠心分離後の状態を示す図である。なお、ここでは検体として血液を用いた場合を例として示している。 Next, the operation and effect when the specimen in the centrifuge container is centrifuged in the centrifuge will be described. FIG. 4 is a diagram showing a state of the centrifuge container when the sample is charged, FIG. 5 is a diagram showing a centrifuge state of the centrifuge container, and FIG. 6 is a centrifuge state of the centrifuge container. FIG. Here, a case where blood is used as a sample is shown as an example.
　図４に示すように、まず遠心分離用容器１の貯留部１１内に血液３０が注入される。具体的には、容器本体１０の上面に設けられた開口１２から、ピペットや注射器等により血液３０が注入される。血液３０の注入後、開口１２に密閉蓋２０が取り付けられる。 As shown in FIG. 4, first, blood 30 is injected into the storage part 11 of the centrifuge container 1. Specifically, blood 30 is injected from the opening 12 provided on the upper surface of the container body 10 by a pipette, a syringe or the like. After the blood 30 is injected, the sealing lid 20 is attached to the opening 12.
　次に、血液３０が注入された遠心分離用容器１は、遠心分離装置５０の収容部５２に収納され、遠心分離用容器１の中心軸Ｃを回転軸として回転させられる。すると、図５に示すように、分離用ゲル１４よりも高い比重の血球成分３１のみがトラップ部１３において分離用ゲル１４よりも外周側に移動し、分離用ゲル１４よりも低い比重の血漿成分３２は分離用ゲル１４よりも内周側に張り付く。 Next, the centrifuge container 1 into which the blood 30 has been injected is accommodated in the accommodating part 52 of the centrifuge 50 and rotated about the central axis C of the centrifuge container 1 as a rotation axis. Then, as shown in FIG. 5, only the blood cell component 31 having a specific gravity higher than that of the separation gel 14 moves to the outer peripheral side of the separation gel 14 in the trap portion 13, and the plasma component having a specific gravity lower than that of the separation gel 14. 32 sticks to the inner peripheral side of the separation gel 14.
　このとき、貯留部１１内の血液３０がエアロゾル化して開口１２から外部に飛散するおそれがあるが、本実施の形態においては、開口１２に密閉蓋２０が取り付けられているため、エアロゾル化した血液３０が容器外部に飛散するのを防止することができる。 At this time, the blood 30 in the reservoir 11 may be aerosolized and scattered from the opening 12 to the outside. However, in the present embodiment, since the sealing lid 20 is attached to the opening 12, the aerosolized blood It is possible to prevent 30 from being scattered outside the container.
　そして、遠心分離用容器１の回転を停止させると、図６に示すように、分離用ゲル１４よりも外周側の血球成分３１はトラップ部１３においてトラップされたまま、分離用ゲル１４よりも内周側の血漿成分３２のみが貯留部１１の下面中心付近に降りて貯留される。これにより血漿成分３２のみを抽出することができる。 Then, when the rotation of the centrifuge container 1 is stopped, as shown in FIG. 6, the blood cell component 31 on the outer peripheral side of the separation gel 14 remains trapped in the trap part 13 and is more internal than the separation gel 14. Only the plasma component 32 on the circumferential side descends and is stored near the center of the lower surface of the storage unit 11. Thereby, only the plasma component 32 can be extracted.
　また、遠心分離後の遠心分離用容器１から血漿成分３２を自動的に分注する場合には、密閉蓋２０を外す必要がなく、分注チップ４０をそのまま密閉蓋２０のチップ挿入口２１に挿入させ、シール部材２２を貫通させて、貯留部１１内の血漿成分３２の分注を行うことができるので、蓋を自動的に開栓するような装置を用いることなく、血漿成分３２の自動分注に対応させることができる。 Further, when the plasma component 32 is automatically dispensed from the centrifuge container 1 after centrifugation, it is not necessary to remove the sealing lid 20, and the dispensing tip 40 is directly put into the chip insertion port 21 of the sealing lid 20. Since the plasma component 32 in the reservoir 11 can be dispensed by being inserted and penetrating the seal member 22, the plasma component 32 can be automatically dispensed without using a device that automatically opens the lid. Can correspond to dispensing.
　以上、本発明の好ましい実施の形態について説明したが、本発明は上記実施の形態に限定されるものではない。 The preferred embodiment of the present invention has been described above, but the present invention is not limited to the above embodiment.
　例えば、シール部材については、上記のように一度貫通させたら修復不可能なシート状部材とするのではなく、可撓性シートにセプタムまたはピンホールが形成されたものとすれば、密閉蓋を再利用することが可能となる。 For example, if the sealing member is not a sheet-like member that cannot be repaired once penetrated as described above, but the septum or pinhole is formed in the flexible sheet, the sealing lid is re-opened. It can be used.
　また、上記以外にも、本発明の要旨を逸脱しない範囲において、各種の改良や変形を行なってもよいのは勿論である。 Of course, in addition to the above, various improvements and modifications may be made without departing from the scope of the present invention.

Claims (3)

  1. 　容器内部に検体を貯留する貯留部を備えるとともに、前記容器に前記検体を前記貯留部内に注入するための開口が設けられ、前記容器の中心軸を回転軸として回転させられることにより前記貯留部内の検体の成分を遠心分離するための遠心分離用容器であって、
    　前記開口を塞ぐ、装着可能な密閉蓋をさらに備え、
    　該密閉蓋は、前記開口に装着された際に、分注チップにより貫通可能であり、貫通した分注チップにより前記貯留部内の検体を採取可能とするシール部材を有する
    　ことを特徴とする遠心分離用容器。     The container is provided with a reservoir for storing the specimen, and an opening for injecting the specimen into the reservoir is provided in the container, and the container is rotated around the central axis of the container as a rotation axis. A centrifuge container for centrifuging the components of a specimen,
    Further comprising a sealable lid that closes the opening,
    The centrifugal lid characterized by having a sealing member that can be penetrated by a dispensing tip when the sealing lid is attached to the opening, and that can collect a sample in the storage part by the penetrating dispensing tip. Container.
  2. 　前記開口が、前記容器の中心軸上にある
    　ことを特徴とする請求項１記載の遠心分離用容器。     The centrifuge container according to claim 1, wherein the opening is on a central axis of the container.
  3. 　前記シール部材が、可撓性シートにセプタムまたはピンホールが形成されたものである
    　ことを特徴とする請求項１または２記載の遠心分離用容器。     The centrifuge container according to claim 1 or 2, wherein the sealing member is a flexible sheet in which a septum or a pinhole is formed.
PCT/JP2013/005498 2012-09-28 2013-09-18 Centrifugation container WO2014050021A1 (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014208330A (en) * 2013-03-29 2014-11-06 富士フイルム株式会社 Centrifugal separation container, centrifugal separator, and centrifugal separation method using the same
WO2017100798A1 (en) 2015-12-11 2017-06-15 Siemens Healthcare Diagnostics Inc. Specimen container and method for separating serum or plasma from whole blood
EP3414015A4 (en) * 2015-06-23 2020-07-22 Karilahti, Mika Centrifuge and system for bodily fluid sample
US12025629B2 (en) 2022-04-06 2024-07-02 Babson Diagnostics, Inc. Automated centrifuge loader
US12050052B1 (en) 2021-08-06 2024-07-30 Babson Diagnostics, Inc. Refrigerated carrier device for biological samples

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49133965A (en) * 1972-11-03 1974-12-23
JPH10512960A (en) * 1995-10-03 1998-12-08 ベックマン インスツルメンツ インコーポレーテッド Blood separation apparatus and method for rotation around an axis
JP2000117150A (en) * 1998-10-16 2000-04-25 Kdk Corp Centrifugal separation method and centrifugal container
JP2004053301A (en) * 2002-07-17 2004-02-19 Yasui Kikai Kk Method for treating crushed specimen and crushing container used therefor
JP2010517022A (en) * 2007-01-25 2010-05-20 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト Apparatus and method for separating liquid components of a blood sample, and analytical apparatus comprising such an apparatus

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49133965A (en) * 1972-11-03 1974-12-23
JPH10512960A (en) * 1995-10-03 1998-12-08 ベックマン インスツルメンツ インコーポレーテッド Blood separation apparatus and method for rotation around an axis
JP2000117150A (en) * 1998-10-16 2000-04-25 Kdk Corp Centrifugal separation method and centrifugal container
JP2004053301A (en) * 2002-07-17 2004-02-19 Yasui Kikai Kk Method for treating crushed specimen and crushing container used therefor
JP2010517022A (en) * 2007-01-25 2010-05-20 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト Apparatus and method for separating liquid components of a blood sample, and analytical apparatus comprising such an apparatus

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014208330A (en) * 2013-03-29 2014-11-06 富士フイルム株式会社 Centrifugal separation container, centrifugal separator, and centrifugal separation method using the same
EP3414015A4 (en) * 2015-06-23 2020-07-22 Karilahti, Mika Centrifuge and system for bodily fluid sample
WO2017100798A1 (en) 2015-12-11 2017-06-15 Siemens Healthcare Diagnostics Inc. Specimen container and method for separating serum or plasma from whole blood
CN108601565A (en) * 2015-12-11 2018-09-28 西门子医疗保健诊断公司 Sample container and method for detaching serum or blood plasma from whole blood
EP3386391A4 (en) * 2015-12-11 2018-10-17 Siemens Healthcare Diagnostics Inc. Specimen container and method for separating serum or plasma from whole blood
US10870110B2 (en) 2015-12-11 2020-12-22 Babson Diagnostics, Inc. Specimen container and centrifugation method for separating serum or plasma from whole blood therewith
CN108601565B (en) * 2015-12-11 2021-09-07 巴布森诊断公司 Sample container and method for separating serum or plasma from whole blood
US11697114B2 (en) 2015-12-11 2023-07-11 Babson Diagnostics, Inc. Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells
US12059676B1 (en) 2015-12-11 2024-08-13 Babson Diagnostics, Inc. Device and method for testing serum and plasma separated from blood cells in whole blood samples
US12050052B1 (en) 2021-08-06 2024-07-30 Babson Diagnostics, Inc. Refrigerated carrier device for biological samples
US12025629B2 (en) 2022-04-06 2024-07-02 Babson Diagnostics, Inc. Automated centrifuge loader

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