WO2011071577A1 - Anticorps anti-vegf-c et leurs procédés d'utilisation - Google Patents

Anticorps anti-vegf-c et leurs procédés d'utilisation Download PDF

Info

Publication number
WO2011071577A1
WO2011071577A1 PCT/US2010/048908 US2010048908W WO2011071577A1 WO 2011071577 A1 WO2011071577 A1 WO 2011071577A1 US 2010048908 W US2010048908 W US 2010048908W WO 2011071577 A1 WO2011071577 A1 WO 2011071577A1
Authority
WO
WIPO (PCT)
Prior art keywords
seq
amino acid
hvr
acid sequence
antibody
Prior art date
Application number
PCT/US2010/048908
Other languages
English (en)
Inventor
Anil D. Bagri
Germaine Fuh
Chingwei V. Lee
Original Assignee
Genentech, Inc.
F. Hoffmann-La Roche Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech, Inc., F. Hoffmann-La Roche Ag filed Critical Genentech, Inc.
Priority to ES10757138.2T priority Critical patent/ES2565208T3/es
Priority to EP10757138.2A priority patent/EP2509626B1/fr
Priority to JP2012543094A priority patent/JP5818805B2/ja
Publication of WO2011071577A1 publication Critical patent/WO2011071577A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A61K2039/507—Comprising a combination of two or more separate antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"

Definitions

  • the present invention concerns VEGF-C antagonists, especially anti-VEGF-C antibodies, and their use in the prevention and treatment of tumor progression.
  • Vascular tube formation is a complex but orderly biological event involving all or many of the following steps: a) Endothelial cells (ECs) proliferate from existing ECs or differentiate from progenitor cells; b) ECs migrate and coalesce to form cord-like structures; c) vascular cords then undergo tubulogenesis to form vessels with a central lumen d) existing cords or vessels send out sprouts to form secondary vessels; e) primitive vascular plexus undergo further remodeling and reshaping; and f) peri-endothelial cells are recruited to encase the endothelial tubes, providing maintenance and modulatory functions to the vessels; such cells including pericytes for small capillaries, smooth muscle cells for larger vessels, and myocardial cells in the heart.
  • ECs Endothelial cells proliferate from existing ECs or differentiate from progenitor cells
  • b) ECs migrate and coalesce to form cord-like structures
  • angiogenesis is implicated in the pathogenesis of a variety of disorders. These include solid tumors and metastasis, atherosclerosis, retrolental fibroplasia, hemangiomas, chronic inflammation, intraocular neovascular diseases such as proliferative retinopathies, e.g., diabetic retinopathy, age-related macular degeneration (AMD), neovascular glaucoma, immune rejection of transplanted corneal tissue and other tissues, rheumatoid arthritis, and psoriasis. Folkman et al, J. Biol.
  • tumor grov angiogenesis appears to be crucial for the transition from hyperplasia to neoplasia, and for providing nourishment for the growth and metastasis of the tumor.
  • Folkman et al. Nature 339:58 (1989).
  • the neovascularization allows the tumor cells to acquire a growth advantage and proliferative autonomy compared to the normal cells.
  • a tumor usually begins as a single aberrant cell which can proliferate only to a size of a few cubic millimeters due to the distance from available capillary beds, and it can stay 'dormant' without further growth and dissemination for a long period of time.
  • metastases are responsible for the vast majority, estimated at 90%, of deaths from solid tumors (Gupta and Massague, Cell 127, 679-695 (2006)).
  • the complex process of metastasis involves a series of distinct steps including detachment of tumor cells from the primary tumor, intravasation of tumor cells into lymphatic or blood vessels, and extravasation and growth of tumor cells in secondary sites.
  • Analysis of regional lymph nodes in many tumor types suggests that the lymphatic vasculature is an important route for the dissemination of human cancers.
  • the presence of tumor cells in lymph nodes is an important adverse prognostic factor.
  • lymphangiogenesis can be induced by solid tumors and can promote tumor spread.
  • VEGF vascular endothelial growth factor
  • the invention provides novel anti-VEGF-C antibodies and uses thereof.
  • the present invention is based, at least in part, on experimental results obtained with anti-VEGF-C antibodies. Results obtained indicate that VEGF-C plays a role in angiogenesis as well as in modulating VEGF-C mediated lymphatic endothelial cell (LEC) migration and proliferation. In addition, the results demonstrate that blocking VEGF-C leads to an inhibition of lymphangiogenesis and a reduction in lymph node and distal organ metastasis.
  • VEGF-C antagonist such as VEGF-C antibodies of the invention, as described herein, provide important therapeutic and diagnostic agents for use in targeting pathological conditions associated with activation of VEGF-C receptors.
  • an antibody that binds to VEGF-C or a fragment thereof comprises at least one, two, three, four, five, or six HVRs selected from:
  • an FJVR-L1 comprising the amino acid sequence of RASQDVSTAVA (SEQ ID NO:27);
  • HVR-L3 comprising the amino acid sequence of QQX 1 YX 2 X 3 PX 4 T wherein the HVR-L3 comprises 1-4 (1, 2, 3, or 4) substitutions in any combination of the following positions: Xi is S or T; X 2 is T, N, W, A, I, Y or R; X 3 is T, I or S; and/or X 4 is P, L, T or Y;
  • HVR- HI comprises 1-4 (1, 2, 3, or 4) substitutions in any combination of the following positions: Xi is S or T; X 2 is D, N or Y; X 3 is N, S, or T; and/or X 4 is D or W;
  • an HVR-H2 comprising the amino acid sequence of XiX 2 ISPX 3 X 4 GXs X 6 X 7 YADSVKG wherein the HVR- H2 comprises 1-7 (1, 2, 3, 4, 5, 6, or 7) substitutions in any combination of the following positions: Xi is A or G; X 2 is F, V or W; X 3 is G, S or Y; X 4 is S or V; X 5 is A, F or Y; X 6 is S or T; and/or X 7 is D or Y; and
  • HVR- H3 comprises 1-12 (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12) substitutions in any combination of the following positions: Xi is A, S, T or V; X 2 is D, L or W; X 3 is A, F, R, V, W or Y; X 4 is D, E, G, K or T; X 5 is I, V or Y; X 6 is A, I, D, K, R or Y; X 7 is F or Y; X 8 is A, G or no amino acid at this position; X is F, G, W or no amino acid at this position; X 10 is V, W or no amino acid at this position; Xn is A or no amino acid at this position; and/or X 12 is F, L, M or no amino acid at this position. See Figures 1 and 23-30. [0011] In another embodiment,
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L3 comprising the amino acid sequence of SEQ ID NO:29, 30, 31, 32, 33, 34, 35, 36, 37 or 38.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three variable light (“VL”) HVR sequences selected from SEQ ID NOs:27, 28, and 29.
  • VL variable light
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 30.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 31.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 32.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 33.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 34.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 35.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 36.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 37.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 38.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three variable heavy (“VH") HVR sequences selected from SEQ ID NOs: l, 6, and 21.
  • VH variable heavy
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 29.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 30.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 31.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 32.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 33.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 34.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 35.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 36.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 37.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs: l, 6, and 21, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 38.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:29.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:30.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO: 31.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l
  • an HVR-H2 comprising the amino acid sequence of SEQ ID NO:6
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:32.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:33.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:34.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:35.
  • an antibody that binds to VEGF-C or a fragment thereof comprises: (1) an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:36.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:37.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • an HVR-H1 comprising the amino acid sequence of SEQ ID NO: l;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:38.
  • an antibody that binds to VEGF-C or a fragment thereof comprises at least one, two, three, four, five or six HVRs selected from:
  • HVR-H1 comprising the amino acid sequence of SEQ ID NO:3 or 4;
  • HVR-H3 comprising the amino acid sequence of SEQ ID NO:23, 24, 25 or 26:
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:29 or 39.
  • an antibody that binds to VEGF-C or a fragment thereof is provided, wherein the antibody comprises a light chain comprising at least one, at least two, or all three VL HVR sequences selected from SEQ ID NOs:27, 28, and 29.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs:3, 8, and 26.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain comprising at least one, at least two, or all three VH HVR sequences selected from SEQ ID NOs:3, 8, and 26, and a light chain comprising at least one, at least two, or three VL HVR sequences selected from SEQ ID NOs:27, 28, and 29.
  • an antibody that binds to VEGF-C or a fragment thereof comprises:
  • HVR-H1 comprising the amino acid sequence of SEQ ID NO:3;
  • an HVR-L2 comprising the amino acid sequence of SEQ ID NO:28; and (6) an HVR-L3 comprising the amino acid sequence of SEQ ID NO:29.
  • an antibody that binds to VEGF-C or a fragment thereof wherein the anti-VEGF-C antibody comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:73.
  • an antibody that binds to VEGF-C or a fragment thereof wherein the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:74, 75, 76, 77, 78, 79, 80, 81, 82 or 83.
  • the anti-VEGF-C antibody comprises a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 75, 78 or 82.
  • the anti-VEGF-C antibody comprises a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 75.
  • the anti-VEGF-C antibody comprises a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:78. In another embodiment, the anti-VEGF-C antibody comprises a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 82.
  • an antibody that binds to VEGF-C or a fragment thereof is provided, wherein the light chain variable domain comprises the amino acid sequence of SEQ ID NO:74, 75, 76, 77, 78, 79, 80, 81, 82 or 83.
  • the light chain variable domain comprises the amino acid sequence of SEQ ID NO:75, 78 or 82.
  • the light chain variable domain comprises the amino acid sequence of SEQ ID NO:75.
  • the light chain variable domain comprises the amino acid sequence of SEQ ID NO:78.
  • the light chain variable domain comprises the amino acid sequence of SEQ ID NO: 82.
  • an antibody that binds to VEGF-C or a fragment thereof comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:74, 75, 76, 77, 78, 79, 80, 81, 82 or 83.
  • the anti-VEGF-C antibody comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 75, 78 or 82.
  • the anti-VEGF-C antibody comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 75.
  • the anti-VEGF- C antibody comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:78.
  • the anti-VEGF-C antibody comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:73, and a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 82.
  • an antibody that binds to VEGF-C or a fragment thereof comprises the anti-VEGF-C antibody comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO:74, 75, 76, 77, 78, 79, 80, 81, 82 or 83.
  • the anti-VEGF-C antibody comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO:75, 78 or 82.
  • the anti- VEGF-C antibody comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO: 75.
  • the anti-VEGF- C antibody comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO:78.
  • the anti-VEGF-C antibody comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO:82.
  • an antibody that binds to VEGF-C or a fragment thereof wherein the anti-VEGF-C antibody comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:84.
  • an antibody that binds to VEGF-C or a fragment thereof wherein the anti-VEGF-C antibody comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 84.
  • an antibody that binds to VEGF-C or a fragment thereof wherein the anti-VEGF-C antibody comprises a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 85.
  • an antibody that binds to VEGF-C or a fragment thereof wherein the anti-VEGF-C antibody comprises the light chain variable domain comprises the amino acid sequence of SEQ ID NO: 85.
  • an antibody that binds to VEGF-C or a fragment thereof is provided, wherein the anti-VEGF-C antibody comprises a heavy chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 84, and a light chain variable domain having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 85.
  • an antibody that binds to VEGF-C or a fragment thereof comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 84, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO: 85.
  • an antibody that binds to VEGF-C or a fragment thereof comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO: 74.
  • an antibody that binds to VEGF-C or a fragment thereof comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO:78.
  • an antibody that binds to VEGF-C or a fragment thereof comprises the heavy chain variable domain comprises the amino acid sequence of SEQ ID NO: 73, and the light chain variable domain comprises the amino acid sequence of SEQ ID NO: 82.
  • an antibody that binds to VEGF-C or a fragment thereof is provided, wherein the antibody comprises an amino acid substitution at position 297 to alanine.
  • the anti-VEGF-C antibodies described herein further comprise an amino acid substitution at position 265 to alanine.
  • the anti-VEGF-C antibodies described herein further comprise amino acid substitutions at positions 265 to alanine and at position 297 to alanine.
  • the anti-VEGF-C antibody is a
  • the antibody is an antibody fragment selected from a Fab, Fab'-SH, Fv, scFv, or (Fab') 2 fragment.
  • the antibody is humanized.
  • the antibody is human.
  • at least a portion of the framework sequence is a human consensus framework sequence.
  • the anti-VEGF-C antibody is a bispecific antibody.
  • the bispecific antibody binds VEGF-C and to another antigen.
  • the bispecific antibody binds VEGF-C and VEGF.
  • the bispecific antibody may bind to two different epitopes of VEGF-C.
  • the bispecific antibody comprises a heavy chain variable domain comprising at one, two, or three variable heavy HVR sequences of the invention. In certain embodiments, the bispecific antibody comprises a heavy chain variable domain that binds to the same epitope on VEGF-C as the anti-VEGF-C antibodies of the invention.
  • the anti-VEGF-C antibody is selected from the group consisting of antibodies VC1, VC1.1, VC1.2, VC1.3, VC1.4, VC1.5, VC1.6, VC1.7, VC1.8, VC1.9, VC1.10, VC1.11, VC1.12, VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VCl .12.9, VC1.12.10, VC3, VC4, VC4,2, VC4,3, VC4.4, VC4.5, and fragments and variants, such as affinity matured variants thereof.
  • the invention concerns an anti-VEGF-C antibody comprising the heavy and/or light chain variable region sequence of an antibody selected from the group consisting of VC 1 , VC 1.1 , VC 1.2, VC 1.3, VC 1.4, VC1.5, VC1.6, VC1.7, VC1.8, VC1.9, VC1.10, VC1.11, VC1.12, VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VCl .12.9,
  • VC1.12.10 VC3, VC4, VC4,2, VC4,3, VC4.4, VC4.5, and fragments and variants thereof.
  • the invention concerns an anti-VEGF-C antibody comprising the heavy and/or light chain variable region sequences of VC1.12, VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VC 1.12.9, VC 1.12.10, VC4.5 , or a fragment or variant thereof.
  • the invention concerns an anti-VEGF-C antibody comprising the heavy and/or light chain variable region sequences of VC4.5, or a fragment or variant thereof. In certain embodiments, the invention concerns an anti-VEGF-C antibody comprising the heavy and/or light chain variable region sequences of VC 1.12, or a fragment or variant thereof. In certain embodiments, the invention concerns an anti- VEGF-C antibody comprising the heavy and/or light chain variable region sequences of VC1.12.4, or a fragment or variant thereof. In certain embodiments, the invention concerns an anti-VEGF-C antibody comprising the heavy and/or light chain variable region sequences of VC1.12.9, or a fragment or variant thereof.
  • a polynucleotide encoding any of the above antibodies is provided.
  • a vector comprising the polynucleotide is provided.
  • a host cell comprising the vector is provided.
  • the host cell is eukaryotic.
  • the host cell is a CHO cell.
  • a method of making an anti-VEGF-C antibody is provided, wherein the method comprises culturing the host cell under conditions suitable for expression of the polynucleotide encoding the antibody, and isolating the antibody.
  • a method of detecting the presence of VEGF-C in a biological sample comprising contacting the biological sample with an antibody of the invention under conditions permissive for binding of the antibody to VEGF-C, and detecting whether a complex is formed between the antibody and VEGF-C.
  • the method comprises detecting VEGF- C-anti- VEGF-C antibody complex in a biological sample wherein the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO:73 or 84, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO:74, 75, 76, 77, 78, 79, 80, 81, 82, 83 or 85.
  • the method comprises detecting VEGF-C-anti- VEGF-C antibody complex in a biological sample wherein the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO:73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 75.
  • the method comprises detecting VEGF-C-anti- VEGF-C antibody complex in a biological sample wherein the amino acid sequence of the anti- VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO:78.
  • the method comprises detecting VEGF-C-anti- VEGF-C antibody complex in a biological sample wherein the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 82. In yet another embodiment, the method comprises detecting VEGF-C-anti- VEGF-C antibody complex in a biological sample wherein the amino acid sequence of the anti- VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 84, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 85.
  • a method for identifying a patient with a disorder associated with VEGF-C expression comprising contacting a biological sample from the patient having or suspected of having the disorder with an antibody of the invention and detecting VEGF-C-anti-VEGF-C antibody complex in the biological sample, wherein the detection of the VEGF-C-anti-VEGF-C antibody complex indicates that the patient has a disorder associated with VEGF-C expression.
  • the amino acid sequence of the anti- VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO:73 or 84, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 74, 75, 76, 77, 78, 79, 80, 81, 82, 83 or 85.
  • the amino acid sequence of the anti- VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 75.
  • the amino acid sequence of the anti- VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO:78. In one embodiment, the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 82. In one embodiment, the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 84, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 85. In yet another embodiment, the anti- VEGF-C antibody is detectably labeled.
  • the invention further provides immunoconjugates comprising an antibody conjugated to an agent, such as a drug or cytotoxic agent.
  • methods for inhibiting angiogenesis comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein are provided.
  • methods for inhibiting lymphatic endothelial cell migration comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein are provided.
  • methods for inhibiting lymphatic endothelial cell proliferation comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein are provided.
  • methods for inhibiting vascular permeability comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein are provided.
  • methods for inhibiting tumoral lymphangiogenesis comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein are provided.
  • methods for inhibiting tumor metastasis comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein are provided.
  • methods for treating a tumor, cancer, or cell proliferative disorder comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein are provided.
  • the methods further comprise administering to the subject an effective amount of an anti- angiogenic agent.
  • the anti-angiogenic agent is a VEGF antagonist.
  • the VEGF antagonist is an anti-VEGF antibody.
  • the anti-VEGF antibody is bevacizumab.
  • methods for inhibiting angiogenesis comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein and an effective amount of an anti-angiogenic agent are provided.
  • methods for inhibiting lymphatic endothelial cell migration comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein and an effective amount of an anti- angiogenic agent are provided.
  • methods for inhibiting lymphatic endothelial cell proliferation comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein and an effective amount of an anti-angiogenic agent are provided.
  • methods for inhibiting vascular permeability comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein and an effective amount of an anti-angiogenic agent are provided.
  • methods for inhibiting tumoral lymphangiogenesis comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein and an effective amount of an anti-angiogenic agent are provided.
  • methods for inhibiting tumor metastasis comprising administering to a subject an effective amount of any of the anti-VEGF-C antibodies described herein and an effective amount of an anti-angiogenic agent are provided.
  • methods for treating a tumor, cancer, or cell proliferative disorder comprising administering to a subject an effective amount of any of the anti- VEGF-C antibodies described herein and an effective amount of an anti-angiogenic agent are provided.
  • the subject is a human patient, such as a human cancer patient, who may have been diagnosed or may be at risk of developing metastasis.
  • the subject is relapsed from or refractory to a VEGF antagonist.
  • the cancer is selected from the group consisting of carcinoma, lymphoma, blastoma, sarcoma, and leukemia.
  • the cancer is selected from the group consisting of squamous cell cancer, small-cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung, squamous carcinoma of the lung, cancer of the peritoneum, hepatocellular cancer, gastric cancer, gastrointestinal cancer,
  • gastrointestinal stromal cancer pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, hepatoma, breast cancer, colon cancer, colorectal cancer, endometrial or uterine carcinoma, salivary gland carcinoma, kidney or renal cancer, liver cancer, prostate cancer, vulval cancer, thyroid cancer, hepatic carcinoma and various types of head and neck cancer, melanoma, superficial spreading melanoma, lentigo maligna melanoma, acral lentiginous melanomas, nodular melanomas, B-cell lymphoma, chronic lymphocytic leukemia (CLL); acute lymphoblastic leukemia (ALL); Hairy cell leukemia; chronic myeloblastic leukemia; post-transplant lymphoproliferative disorder (PTLD), abnormal vascular proliferation associated with phakomatoses, edema associated with brain tumors, and Meigs' syndrome.
  • CLL chronic lymphocytic leukemia
  • the cancer is non-small cell lung cancer, renal cancer, glioblastoma, breast cancer, ovarian cancer, colon cancer or colorectal cancer.
  • B-cell lymphoma is selected from the group consisting of low grade/follicular non-Hodgkin's lymphoma (NHL); small lymphocytic (SL) NHL; intermediate grade/follicular NHL; intermediate grade diffuse NHL; high grade immunoblastic NHL; high grade lymphoblastic NHL; high grade small non-cleaved cell NHL; bulky disease NHL; mantle cell lymphoma; AIDS-related lymphoma; and Waldenstrom's Macroglobulinemia.
  • NHL low grade/follicular non-Hodgkin's lymphoma
  • SL small lymphocytic
  • a method of enhancing efficacy of an anti- angiogenic agent in a subject having a pathological condition associated with angiogenesis comprising administering to the subject an effective amount of any of the antibodies above in addition to the anti-antigenic agent, thereby enhancing said anti-angiogenic agent's efficacy.
  • the subject is human.
  • the pathological condition associated with angiogenesis is a tumor, a cancer, and/or a cell proliferative disorder.
  • the pathological condition associated with angiogenesis is an intraocular neovascular disease.
  • the anti-angiogenic agent is a VEGF antagonist.
  • the VEGF antagonist is an anti-VEGF antibody.
  • the anti-VEGF antibody is bevacizumab.
  • the invention further concerns a composition comprising any of the anti-VEGF-C antibodies above in admixture with a
  • the invention concerns a pharmaceutical composition for the prevention or treatment of tumor metastasis comprising an effective amount of any of the anti-VEGF-C antibodies described herein in admixture with a pharmaceutically acceptable carrier.
  • methods of treating caner comprising administering to the subject the pharmaceutical composition comprising any of the anti-VEGF-C antibodies described herein.
  • the methods further comprise administering to the subject an effective amount of anti-VEGF antibody.
  • An exemplary and non-limiting list of cancers contemplated is provided herein under "Definitions.”
  • methods for treating a tumor, cancer or cell proliferative disorder in a subject refractory to or relapsed from a VEGF antagonist therapy comprising the step of administering to the subject any of the anti- VEGF-C antibodies described herein alone or in combination with VEGF antagonist are provided.
  • subjects are previously treated with anti- VEGF antibody.
  • the tumors are non-responsive or refractory to anti-VEGF antibody therapy.
  • the invention concerns anti-VEGF-C antibodies described herein for use in the prevention or treatment of tumor metastasis.
  • methods of preventing recurrence of cancer in a subject comprising administering to the subject any of the anti-VEGF-C antibodies described herein, wherein the administering prevents cancer recurrence in the subject are provided.
  • methods of reducing the likelihood of cancer recurrence in a subject comprising administering to the subject any of the anti-VEGF- C antibodies described herein, wherein the administering reduces the likelihood of cancer recurrence in the subject are provided.
  • the administering of any of the anti-VEGF-C antibodies described herein prevents or reduces the likelihood of reoccurrence of a clinically detectable tumor, or metastasis thereof.
  • methods of preventing the regrowth of a tumor in a subject comprising the steps of removing the tumor and thereafter administering to the subject any of the anti-VEGF-C antibodies described herein are provided.
  • methods of preventing the recurrence of cancer in a subject having a tumor comprising the steps of removing the tumor and thereafter administering to the subject any of the anti-VEGF-C antibodies described herein are provided.
  • the amino acid sequence of the anti- VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO:73 or 84, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 74, 75, 76, 77, 78, 79, 80, 81, 82, 83 or 85.
  • the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 75.
  • the amino acid sequence of the anti- VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO:78. In yet another embodiment, the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 73, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 82. In yet another embodiment, the amino acid sequence of the anti-VEGF-C antibody comprises a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 84, and a light chain variable domain comprising the amino acid sequence of SEQ ID NO:85.
  • the anti-VEGF-C antibodies described herein blocks biological activity of VEGF-C.
  • the VEGF-C is the full-length VEGF-C.
  • the VEGF-C is the mature VEGF- C.
  • the methods described above further comprise administering to the subject an effective amount of an anti-angiogenic agent.
  • the anti-angiogenic agent is a VEGF antagonist.
  • the VEGF antagonist is an anti-VEGF antibody.
  • the anti-VEGF antibody is bevacizumab.
  • any of the methods described above further comprises administering to the subject an effective amount of a
  • chemotherapeutic agent An exemplary and non-limiting list of chemotherapeutic agents contemplated is provided herein under "Definitions.”
  • the chemotherapeutic agent is selected from the group consisting of paclitaxel, carboplatin, cisplatin, gemcitabine and pemetrexed.
  • anti-VEGF antibody is administered first to the subject and then anti-VEGF-C antibody is administered to the subject.
  • anti-VEGF-C antibody and anti-VEGF antibody are provided.
  • anti-VEGF-C antibody, anti-VEGF antibody and chemotherapeutic agent are administered simultaneously to the subject.
  • Figure 1A-F Heavy chain and light chain HVR loop sequences of anti-VEGF-C antibodies.
  • the figures show the heavy chain HVR sequences, HI, H2, and H3, and light chain HVR sequences, LI, L2 and L3.
  • Sequence numbering is as follows: clone VC1 (HVR-H1 is SEQ ID NO: l; HVR-H2 is SEQ ID NO:5; HVR-H3 is SEQ ID NO:9; HVR-Ll is SEQ ID NO:27; HVR-L2 is SEQ ID NO:28; HVR-L3 is SEQ ID NO:29); clone VCl .l (HVR-Hl is SEQ ID NO: l; HVR-H2 is SEQ ID NO:5; HVR-H3 is SEQ ID NO: 10; HVR-L1 is SEQ ID NO:27; HVR-L2 is SEQ ID NO:28; HVR-L3 is SEQ ID NO:29); clone VC1.2
  • HVR-H2 is SEQ ID NO:8; HVR-H3 is SEQ ID NO:23; HVR-L1 is SEQ ID NO:27; HVR-L2 is SEQ ID NO:28; HVR-L3 is SEQ ID NO:29); clone VC4.2 (HVR-Hl is SEQ ID NO:3; HVR-H2 is SEQ ID NO:8; HVR-H3 is SEQ ID NO:24; HVR-L1 is SEQ ID NO:27; HVR-L2 is SEQ ID NO:28; HVR-L3 is SEQ ID NO:29); clone VC4.3 (HVR-Hl is SEQ ID NO:4; HVR-H2 is SEQ ID NO:91; HVR-H3 is SEQ ID NO:23; HVR-L1 is SEQ ID NO:27; HVR-L2 is SEQ ID NO:28; HVR-L3 is SEQ ID NO:39); clone VC4.4 (HVR-Hl is SEQ ID
  • Figures 2A & 2B depict exemplary acceptor human consensus framework sequences for use in practicing the instant invention with sequence identifiers as follows:
  • VH Variable heavy consensus framework
  • Figure 3 depicts exemplary acceptor human consensus framework sequences for use in practicing the instant invention with sequence identifiers as follows:
  • VL Variable light consensus framework
  • Figure 4 depicts framework region sequences of huMAb4D5-8 heavy and light chains. Numbers in superscript/bold indicate amino acid positions according to Kabat.
  • Figure 5 depicts modified/variant framework region sequences of huMAb4D5-8 heavy and light chains. Numbers in superscript/bold indicate amino acid positions according to Kabat.
  • FIG. 6 A. Schematic of VEGF-C including VEGF homology domain (VHD).
  • Figure 7 Anti-VEGF-C antibody reduces VEGF-C-induced cellular migration in vitro.
  • A. LECs migrating in response to 200 ng/ml of mature VEGF-C (R&D Systems) for 18 hours in the presence or absence of anti-VEGF-C (10 ⁇ g/ml) (n 6 for each condition).
  • FIG. 8 Anti-VEGF-C antibody reduces VEGF-C-induced cellular proliferation in vitro.
  • FIG. 9 Anti-VEGF-C antibody reduces VEGF-C-induced sprouting in vitro. LECs sprouting in a bead sprouting assay in response to 200 ng/ml of mature VEGF-C (R&D Systems) for 14 days in the presence or absence of anti- VEGF-C antibody (10 ⁇ g/ml). Cultures were stained with anti-LYVE-1 antibody to visualize LECs (representative images shown).
  • FIG. 10 Anti-VEGF-C antibody VC4.5 blocks VEGF-C binding to VEGFR3. Receptor blocking activities comparing the anti-VEGF-C antibody VC4.5 as murine IgG2a or human IgGl form. Fixed concentration of biotin conjugated VEGF-C was incubated with serial dilutions of anti-VEGF-C antibody mVC4.5 (mIgG2a) and anti-VEGF-C antibody hVC4.5 (hlgGl) for 2 hours. The unbound VEGF-C was then captured by immobilized VEGFR3-Fc and detected with strepavidin-HRP conjugate.
  • FIG 11 Anti-VEGF-C antibody reduces VEGF-C-induced VEGFR2 activation in vitro. Receptor activation as assessed by pVEGFR2 production in response to 10 min of VEGF-A (20 ng/ml) or VEGF-C (200 ng/ml, R&D Systems) stimulation in the presence or absence of anti-VEGF-C antibody (10 ⁇ ). Values are normalized to total VEGFR2 levels. pVEGFR2 and total VEGFR2 detected using ELISA kits (R&D Systems).
  • FIG. 13 Anti-VEGF-C antibody reduces VEGF-C-induced function in vivo.
  • the staining can be quantified as show in the panels below - areas to be included as positive in the analysis have been pseudocolored in the lower panels.
  • FIG. 14 Anti-VEGF-C antibody reduces VEGF-C-induced function in vivo. Quantification of the pixel counts evaluating (A) angiogenesis and (B) lymphangiogenesis from systemic treatment with anti-VEGF-C antibody (10 mg/kg twice weekly). *p ⁇ 0.05; Error bars represent standard error of the mean.
  • FIG. 15 Anti-VEGF-C antibody treatment results in a reduction of tumor angiogenesis in orthotopically grown 66c 14 tumors.
  • A Representative images of PECAM-1 stained vessels in 66c 14 tumors treated with isotype specific control (anti-Ragweed) antibody (top), or anti-VEGF-C antibody (bottom).
  • FIG. 16 Anti-VEGF-C antibody treatment results in a reduction of tumor growth in 66c 14 tumors as a single agent or in combination with anti-VEGF-
  • B Mean tumor volume graph of an independent 66c 14 tumor model study. Animals were dosed twice weekly i.p. with anti-VEGF-C (10 mg/kg), anti-VEGF-A antibody (5mg/kg), or a combination of both agents, once tumors reached an average size of 100 mm and were dosed throughout the study.
  • FIG. 17 Anti-VEGF-C antibody treatment results in a increased survival in H460 tumors as a single agent or in combination with anti-VEGF-A antibody.
  • FIG. 18 Anti-VEGF-C antibody treatment results in a reduction of tumor angiogenesis in H460 tumors as a single agent or in combination with anti- VEGF-A antibody.
  • B Quantification of vascular vessel density was determined from 6 representative images from each of 6 tumors per group, evaluated for mean pixel number by ImageJ. Tumors were evaluated at study endpoint. *p ⁇ 0.05; Error bars represent standard error of the mean.
  • Figure 19 Comparison of in vivo efficacy of the murine and human backbone anti-VEGF-C antibodies. Independent 66cl4 tumor model studies were run with either A. murine or B. human backbone anti-VEGF-C antibody.
  • FIG. 20 Anti-VEGF-C antibody treatment results in a reduction of lung metastasis in 66c 14 tumors.
  • A Representative images of lungs from control (left) and anti-VEGF-C antibody (right) treated animals. Lungs were inflated prior to fixation by right cardiac ventricular perfusion. Nodules are highlighted in white to facilitate visualization.
  • B Quantification by visual inspection of the number of metastatic nodules per lung in control and anti-VEGF-C antibody treated animals.
  • Figure 21 A. Anti-VEGF-C antibody treatment results in a reduction of lung metastasis in 66c 14 tumors. Three-dimensional renderings of representative micro-CT scanned lungs demonstrating metastatic nodules (red) in control and anti-VEGF-C antibody treated animals. B. H&E staining of a lung nodule (arrow) demonstrating metastatic tumor cells. *p ⁇ 0.05; Error bars represent standard error of the mean.
  • FIG. 22 Anti-VEGF-C antibody treatment results in a reduction of tumor lymphatic vessels.
  • A Representative images of LYVE-1 stained lymphatic vessels in 66c 14 (top row) and C6 (bottom row) tumors treated with control antibody (left column) or anti-VEGF-C antibody (right column).
  • B Quantification of lymphatic vessel density was determined from 6 representative images from each of 6 tumors per group, evaluated for mean pixel number by ImageJ. *p ⁇ 0.05; Error bars represent standard error of the mean.
  • Figure 23 The LI, L2 and L3 amino acid sequences for anti- VEGF-C antibodies VC1, VC3 and VC4.
  • Figure 24 The HI, H2 and H3 amino acid sequences for anti- VEGF-C antibodies VC1, VC3 and VC4.
  • Figure 25 The LI, L2 and L3 amino acid sequences for anti- VEGF-C antibodiesVC4, VC4.2, VC4.3, VC4.4 and VC4.5.
  • Figure 26 The HI, H2 and H3 amino acid sequences for anti- VEGF-C antibodiesVC4, VC4.2, VC4.3, VC4.4 and VC4.5.
  • Figure 27 The LI, L2 and L3 amino acid sequences for anti- VEGF-C antibodies VC1, VC1.1, VC1.2, VC1.3, VC1.4, VC1.5, VC1.6, VC1.7, VC1.8, VC1.9, VC1.10, VC1.11 and VC1.12.
  • Figure 28 The HI, H2 and H3 amino acid sequences for anti- VEGF-C antibodies VC1, VC1.1, VC1.2, VC1.3, VC1.4, VC1.5, VC1.6, VC1.7, VC1.8, VC1.9, VC1.10, VC1.11 and VC1.12.
  • Figure 29 The LI, L2 and L3 amino acid sequences for anti- VEGF-C antibodies VC1.12, VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VCl .12.9 and VC1.12.10.
  • Figure 30 The HI, H2 and H3 amino acid sequences for anti- VEGF-C antibodies VC1.12, VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VCl .12.9 and VC1.12.10.
  • Figure 31 Table summarizing binding affinity data of anti- VEGF-C antibodies VC1, VC3 and VC4.
  • Figure 32 Receptor blocking activities of anti- VEGF-C IgGs VC1, VC3 and VC4.
  • Anti-VEGF-C IgGs VC1, VC3 and VC4 block biotinylated- human VEGF-C binding to VEGFR3 coated plate in dose-dependent manner.
  • Figure 33 Table summarizing the Phage IC 50 data of VC4 affinity improved variants (VC4.2, VC4.3, VC4.4 and VC4.5) to human VEGF-C.
  • Figure 34 Table summarizing the kinetic binding affinity measurement of anti- VEGF-C antibody VC4.5 Fab protein and anti- VEGF-C antibody VC4.5 IgG to human VEGF-C (R&D systems) and human VEGF-C C137S.
  • Figure 35 Table summarizing the Phage IC 50 data of VC1 affinity improved variants (VC1.1 , VC 1.2, VC 1.3, VC 1.4, VC 1.5, VC 1.6, VC 1.7, VC 1.8, VC 1.9, VC 1.10, VC 1.11 and VC 1.12) to human VEGF-C.
  • Figure 36 Table summarizing the Phage IC 50 data of VC 1.12 affinity improved variants (VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VC1.12.9 and VC1.12.10) to human VEGF-C C137S.
  • Figure 37 Epitope mapping of anti-VEGF-C antibodies.
  • VC4 has different epitope to VC3 and VC1 series antibodies.
  • Figure 38 Depicts the light chain variable regions of anti-VEGF- C antibodies VC1.12 and VC4.5.
  • Figure 39 Depicts the heavy chain variable regions of anti- VEGF-C antibodies VC1.12 and VC4.5.
  • Figure 40 Cell proliferation assay - anti-VEGF-C antibodies reduce VEGF-C-induced cellular proliferation in vitro.
  • FIG. 41 Anti-VEGF-C antibody inhibits VEGF-C mediated phosphorylation of VEGFR3.
  • VEGFR3 phosphorylation level as assayed by the VEGFR3 KIRA assay.
  • Figure 42 Treatment with anti-VEGF-C antibody reduced VEGF- C induced vascular permeability. Quantification of results from mouse skin vascular permeability assay. The quantification was determined from the Evan's blue dye extracted from skin samples in the permeability assay. Animals were treated with anti-VEGF-C antibody (0.5mg/ml) or VEGFR3 ECD (l .Omg/ml). Values shown are the average of 6 independent experiments. *p ⁇ 0.05; Error bars represent standard error of the mean.
  • Figure 43 Concomitant inhibition of VEGF-C and VEGF provides additional benefit for primary tumor growth stasis.
  • Figure 44 Comparison of in vivo efficacy of the mouse backbone anti-VEGF-C antibodies using 66c 14 tumor model.
  • Figure 45 Comparison of anti-VEGF-C antibodies in a proliferation assay. LECs proliferation in response to 200 ng/ml of mature VEGF-C in the presence or absence of different doses of anti-VEGF-C antibodies.
  • Figure 46 Anti-VEGF-C treatment results in a reduction of number of metastatic tumor lesions in mice.
  • the invention herein provides isolated antibodies that bind to VEGF-C, that are useful for, e.g., diagnosis, treatment or prevention of disease states associated with activity of VEGF-C.
  • Pharmaceutical compositions as well as methods of treatment are also provided.
  • the antibodies of the invention are used to treat a tumor, a cancer, and/or a cell proliferative disorder.
  • the antibodies of the invention are used to treat a pathological condition associated with lymphangiogenesis and angiogenesis.
  • the anti-VEGF-C antibodies of the invention find utility as reagents for detection and/or isolation of VEGF-C, such as detection of VEGF-C in various tissues and cell type.
  • the invention further provides methods of making anti-VEGF-C antibodies, and polynucleotides encoding anti-VEGF-C antibodies.
  • antibody herein is used in the broadest sense and encompasses various immunoglobulin structures, including but not limited to monoclonal antibodies, polyclonal antibodies, multispecific antibodies, and antibody fragments so long as they exhibit the desired antigen-binding activity.
  • the term "monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible mutations, e.g., naturally occurring mutations, that may be present in minor amounts. Thus, the modifier “monoclonal” indicates the character of the antibody as not being a mixture of discrete antibodies.
  • such a monoclonal antibody typically includes an antibody comprising a polypeptide sequence that binds a target, wherein the target-binding polypeptide sequence was obtained by a process that includes the selection of a single target binding polypeptide sequence from a plurality of polypeptide sequences.
  • the selection process can be the selection of a unique clone from a plurality of clones, such as a pool of hybridoma clones, phage clones, or recombinant DNA clones.
  • a selected target binding sequence can be further altered, for example, to improve affinity for the target, to humanize the target binding sequence, to improve its production in cell culture, to reduce its immunogenicity in vivo, to create a multispecific antibody, etc., and that an antibody comprising the altered target binding sequence is also a monoclonal antibody of this invention.
  • polyclonal antibody In contrast to polyclonal antibody
  • each monoclonal antibody of a monoclonal antibody preparation is directed against a single determinant on an antigen.
  • monoclonal antibody preparations are advantageous in that they are typically uncontaminated by other immunoglobulins.
  • the modifier "monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method.
  • the monoclonal antibodies to be used in accordance with the present invention may be made by a variety of techniques, including, for example, the hybridoma method (e.g., Kohler and Milstein, Nature, 256:495-97 (1975); Hongo et al, Hybridoma, 14 (3): 253-260 (1995), Harlow et al, Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, 2nd ed.
  • the monoclonal antibodies herein specifically include "chimeric" antibodies in which a portion of the heavy and/or light chain is identical with or homologous to corresponding sequences in antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical with or homologous to corresponding sequences in antibodies derived from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity (see, e.g., U.S. Patent No. 4,816,567; and Morrison et al, Proc. Natl. Acad. Sci. USA 81 :6851-6855 (1984)).
  • Chimeric antibodies include PRIMATIZED® antibodies wherein the antigen-binding region of the antibody is derived from an antibody produced by, e.g., immunizing macaque monkeys with the antigen of interest.
  • Humanized forms of non-human (e.g., murine) antibodies are chimeric antibodies that contain minimal sequence derived from non-human immunoglobulin.
  • a humanized antibody is a human
  • immunoglobulin in which residues from a HVR of the recipient are replaced by residues from a HVR of a non-human species (donor antibody) such as mouse, rat, rabbit, or nonhuman primate having the desired specificity, affinity, and/or capacity.
  • donor antibody such as mouse, rat, rabbit, or nonhuman primate having the desired specificity, affinity, and/or capacity.
  • FR residues of the human immunoglobulin are replaced by corresponding non-human residues.
  • humanized antibodies may comprise residues that are not found in the recipient antibody or in the donor antibody. These modifications may be made to further refine antibody performance.
  • a humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the hypervariable loops correspond to those of a non-human immunoglobulin, and all or substantially all of the FRs are those of a human immunoglobulin sequence.
  • the humanized antibody optionally will also comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin.
  • Fc immunoglobulin constant region
  • a "human antibody” is one which possesses an amino acid sequence which corresponds to that of an antibody produced by a human and/or has been made using any of the techniques for making human antibodies as disclosed herein. This definition of a human antibody specifically excludes a humanized antibody comprising non-human antigen-binding residues.
  • Human antibodies can be produced using various techniques known in the art, including phage-display libraries. Hoogenboom and Winter, J. Mol. Biol, 227:381 (1991); Marks et al, J. Mol. Biol, 222:581 (1991). Also available for the preparation of human monoclonal antibodies are methods described in Cole et al, Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, p.
  • Human antibodies can be prepared by administering the antigen to a transgenic animal that has been modified to produce such antibodies in response to antigenic challenge, but whose endogenous loci have been disabled, e.g., immunized xenomice (see, e.g., U.S. Pat. Nos. 6,075,181 and 6,150,584 regarding XENOMOUSETM technology). See also, for example, Li et al, Proc. Natl. Acad. Sci. USA, 103:3557-3562 (2006) regarding human antibodies generated via a human B-cell hybridoma technology.
  • a "species-dependent antibody” is one which has a stronger binding affinity for an antigen from a first mammalian species than it has for a homologue of that antigen from a second mammalian species. Normally, the species- dependent antibody "binds specifically" to a human antigen (i.e. has a binding affinity
  • the species-dependent antibody can be any of the various types of antibodies as defined above, but preferably is a humanized or human antibody.
  • antibody mutant refers to an amino acid sequence variant of the species-dependent antibody wherein one or more of the amino acid residues of the species-dependent antibody have been modified. Such mutants necessarily have less than 100% sequence identity or similarity with the species-dependent antibody.
  • the antibody mutant will have an amino acid sequence having at least 75% amino acid sequence identity or similarity with the amino acid sequence of either the heavy or light chain variable domain of the species-dependent antibody, in another embodiment at least 80%, in another embodiment at least 85%, in another embodiment at least 90%, and yet in another embodiment at least 95%. Identity or similarity with respect to this sequence is defined herein as the percentage of amino acid residues in the candidate sequence that are identical (i.e.
  • An "isolated" antibody is one which has been identified and separated and/or recovered from a component of its natural environment.
  • an antibody is purified (1) to greater than 95% by weight of antibody as determined by, for example, the Lowry method, and in some
  • Isolated antibody includes the antibody in situ within recombinant cells since at least one component of the antibody's natural environment will not be present. Ordinarily, however, isolated antibody will be prepared by at least one purification step.
  • “Native antibodies” are usually heterotetrameric glycoproteins of about 150,000 daltons, composed of two identical light (L) chains and two identical heavy (H) chains. Each light chain is linked to a heavy chain by one covalent disulfide bond, while the number of disulfide linkages varies among the heavy chains of different immunoglobulin isotypes. Each heavy and light chain also has regularly spaced intrachain disulfide bridges. Each heavy chain has at one end a variable domain (V H ) followed by a number of constant domains.
  • V H variable domain
  • Each light chain has a variable domain at one end (V L ) and a constant domain at its other end; the constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain. Particular amino acid residues are believed to form an interface between the light chain and heavy chain variable domains.
  • variable region refers to the amino-terminal domains of the heavy or light chain of the antibody.
  • variable domain of the heavy chain may be referred to as "VH.”
  • variable domain of the light chain may be referred to as "VL.” These domains are generally the most variable parts of an antibody and contain the antigen-binding sites.
  • variable refers to the fact that certain portions of the variable domains differ extensively in sequence among antibodies and are used in the binding and specificity of each particular antibody for its particular antigen.
  • variable domains of antibodies differ in their variability. It is concentrated in three segments called hypervariable regions (HVRs) both in the light-chain and the heavy-chain variable domains.
  • HVRs hypervariable regions
  • FR framework regions
  • the variable domains of native heavy and light chains each comprise four FR regions, largely adopting a beta-sheet configuration, connected by three HVRs, which form loops connecting, and in some cases forming part of, the beta-sheet structure.
  • the HVRs in each chain are held together in close proximity by the FR regions and, with the HVRs from the other chain, contribute to the formation of the antigen-binding site of antibodies (see Kabat et al., Sequences of Proteins of Immunological Interest, Fifth Edition, National Institute of Health, Bethesda, MD (1991)).
  • the constant domains are not involved directly in the binding of an antibody to an antigen, but exhibit various effector functions, such as participation of the antibody in antibody-dependent cellular toxicity.
  • the "light chains" of antibodies (immunoglobulins) from any vertebrate species can be assigned to one of two clearly distinct types, called kappa (K) and lambda ( ⁇ ), based on the amino acid sequences of their constant domains.
  • antibodies can be assigned to different classes.
  • immunoglobulins There are five major classes of immunoglobulins: IgA, IgD, IgE, IgG, and IgM, and several of these may be further divided into subclasses (isotypes), e.g., IgGi, IgG 2 , IgG 3 , IgG 4 , IgAi, and IgA 2 .
  • the heavy chain constant domains that correspond to the different classes of immunoglobulins are called ⁇ , ⁇ , ⁇ , ⁇ , and ⁇ , respectively.
  • An antibody may be part of a larger fusion molecule, formed by covalent or non-covalent association of the antibody with one or more other proteins or peptides.
  • full length antibody “intact antibody” and “whole antibody” are used herein interchangeably to refer to an antibody in its substantially intact form, not antibody fragments as defined below.
  • a "naked antibody” for the purposes herein is an antibody that is not conjugated to a cytotoxic moiety or radiolabel.
  • Antibody fragments comprise a portion of an intact antibody, preferably comprising the antigen binding region thereof.
  • Examples of antibody fragments include Fab, Fab', F(ab') 2 , and Fv fragments; diabodies; linear antibodies; single-chain antibody molecules; and multispecific antibodies formed from antibody fragments.
  • Papain digestion of antibodies produces two identical antigen- binding fragments, called “Fab” fragments, each with a single antigen-binding site, and a residual "Fc” fragment, whose name reflects its ability to crystallize readily. Pepsin treatment yields an F(ab') 2 fragment that has two antigen-combining sites and is still capable of cross-linking antigen.
  • Fv is the minimum antibody fragment which contains a complete antigen-binding site.
  • a two-chain Fv species consists of a dimer of one heavy- and one light-chain variable domain in tight, non-covalent association.
  • one heavy- and one light-chain variable domain can be covalently linked by a flexible peptide linker such that the light and heavy chains can associate in a "dimeric" structure analogous to that in a two-chain Fv species. It is in this configuration that the three HVRs of each variable domain interact to define an antigen-binding site on the surface of the VH-VL dimer.
  • variable domain or half of an Fv comprising only three HVRs specific for an antigen
  • the Fab fragment contains the heavy- and light-chain variable domains and also contains the constant domain of the light chain and the first constant domain (CHI) of the heavy chain.
  • Fab' fragments differ from Fab fragments by the addition of a few residues at the carboxy terminus of the heavy chain CHI domain including one or more cysteines from the antibody hinge region.
  • Fab'-SH is the designation herein for Fab' in which the cysteine residue(s) of the constant domains bear a free thiol group.
  • F(ab') 2 antibody fragments originally were produced as pairs of Fab' fragments which have hinge cysteines between them. Other chemical couplings of antibody fragments are also known.
  • Single-chain Fv or “scFv” antibody fragments comprise the VH and VL domains of antibody, wherein these domains are present in a single polypeptide chain.
  • the scFv polypeptide further comprises a polypeptide linker between the VH and VL domains which enables the scFv to form the desired structure for antigen binding.
  • diabodies refers to antibody fragments with two antigen-binding sites, which fragments comprise a heavy-chain variable domain (VH) connected to a light-chain variable domain (VL) in the same polypeptide chain (VH- VL).
  • VH heavy-chain variable domain
  • VL light-chain variable domain
  • Diabodies may be bivalent or bispecific. Diabodies are described more fully in, for example, EP 404,097; WO 1993/01161; Hudson et al, Nat. Med. 9: 129-134 (2003); and Hollinger et al., Proc. Natl. Acad. Sci. USA 90: 6444-6448 (1993). Triabodies and tetrabodies are also described in Hudson et al, Nat. Med. 9: 129-134 (2003).
  • hypervariable region when used herein refers to the regions of an antibody variable domain which are hypervariable in sequence and/or form structurally defined loops.
  • antibodies comprise six HVRs; three in the VH (HI, H2, H3), and three in the VL (LI, L2, L3).
  • H3 and L3 display the most diversity of the six HVRs, and H3 in particular is believed to play a unique role in conferring fine specificity to antibodies.
  • HVR delineations are in use and are encompassed herein.
  • the Kabat Complementarity Determining Regions are based on sequence variability and are the most commonly used (Kabat et al, Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD. (1991)). Chothia refers instead to the location of the structural loops (Chothia and Lesk J. Mol. Biol. 196:901-917 (1987)).
  • the AbM HVRs represent a compromise between the Kabat HVRs and Chothia structural loops, and are used by Oxford Molecular's AbM antibody modeling software.
  • the "contact" HVRs are based on an analysis of the available complex crystal structures. The residues from each of these HVRs are noted below.
  • HVRs may comprise "extended HVRs” as follows: 24-36 or 24-34 (LI), 46-56 or 50-56 (L2) and 89-97 or 89-96 (L3) in the VL and 26-35 (HI), 50-65 or 49-65 (H2) and 93-102, 94-102, or 95-102 (H3) in the VH.
  • the variable domain residues are numbered according to Kabat et al, supra, for each of these definitions.
  • variable domain residue numbering as in Kabat or "amino acid position numbering as in Kabat,” and variations thereof, refers to the numbering system used for heavy chain variable domains or light chain variable domains of the compilation of antibodies in Kabat et al., supra. Using this numbering system, the actual linear amino acid sequence may contain fewer or additional amino acids corresponding to a shortening of, or insertion into, a FR or HVR of the variable domain.
  • a heavy chain variable domain may include a single amino acid insert (residue 52a according to Kabat) after residue 52 of H2 and inserted residues (e.g. residues 82a, 82b, and 82c, etc. according to Kabat) after heavy chain FR residue 82.
  • the Kabat numbering of residues may be determined for a given antibody by alignment at regions of homology of the sequence of the antibody with a "standard" Kabat numbered sequence.
  • the Kabat numbering system is generally used when referring to a residue in the variable domain (approximately residues 1-107 of the light chain and residues 1-113 of the heavy chain) (e.g., Kabat et al, Sequences of Immunological Interest. 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)).
  • the "EU numbering system” or "EU index” is generally used when referring to a residue in an immunoglobulin heavy chain constant region (e.g., the EU index reported in Kabat et al., supra).
  • the "EU index as in Kabat” refers to the residue numbering of the human IgGl EU antibody.
  • references to residue numbers in the variable domain of antibodies means residue numbering by the Kabat numbering system. Unless stated otherwise herein, references to residue numbers in the constant domain of antibodies means residue numbering by the EU numbering system (e.g., see United States Publication No. 2008/0181888, Figures for EU numbering).
  • multispecific antibody is used in the broadest sense and specifically covers an antibody that has polyepitopic specificity.
  • Such multispecific antibodies include, but are not limited to, an antibody comprising a heavy chain variable domain (V H ) and a light chain variable domain (V L ), wherein the V H V L unit has polyepitopic specificity, antibodies having two or more V L and V H domains with each V H V L unit binding to a different epitope, antibodies having two or more single variable domains with each single variable domain binding to a different epitope, full length antibodies, antibody fragments such as Fab, Fv, dsFv, scFv, diabodies, bispecific diabodies and triabodies and antibody fragments that have been linked covalently or non-covalently.
  • the multispecific antibody is an IgG antibody that binds to each epitope with an affinity of 5 ⁇ to 0.001 pM, 3 ⁇ to 0.001 pM, 1 ⁇ to 0.001 pM, 0.5 ⁇ to 0.001 pM, or 0.1 ⁇ to 0.001 pM.
  • Polyepitopic specificity refers to the ability to specifically bind to two or more different epitopes on the same or different antigen(s).
  • bispecific refers to the ability to bind two different epitopes.
  • “Monospecific” refers to the ability to bind only one epitope.
  • single domain antibodies or “single variable domain (SVD) antibodies” generally refers to antibodies in which a single variable domain (V H or V L ) can confer antigen binding. In other words, the single variable domain does not need to interact with another variable domain in order to recognize the target antigen.
  • single domain antibodies include those derived from camelids (lamas and camels) and cartilaginous fish (e.g., nurse sharks) and those derived from recombinant methods from humans and mouse antibodies (Nature (1989) 341 :544-546; Dev Comp Immunol (2006) 30:43-56; Trend Biochem Sci (2001) 26:230-235; Trends Biotechnol (2003):21 :484-490; WO 2005/035572; WO 03/035694; Febs Lett (1994) 339:285-290; WO00/29004; WO 02/051870).
  • linear antibodies generally refers to the antibodies described in Zapata et al, Protein Eng. 8(10): 1057-1062 (1995). Briefly, these antibodies comprise a pair of tandem Fd segments (V H -C H 1-V H -C H 1) which, together with complementary light chain polypeptides, form a pair of antigen binding regions. Linear antibodies can be bispecific or monospecific.
  • an "affinity matured" antibody is one with one or more alterations in one or more HVRs thereof which result in an improvement in the affinity of the antibody for antigen, compared to a parent antibody which does not possess those alteration(s).
  • an affinity matured antibody has nanomolar or even picomolar affinities for the target antigen.
  • Affinity matured antibodies may be produced using certain procedures known in the art. For example, Marks et al.
  • a “blocking” antibody or an “antagonist” antibody is one which inhibits or reduces biological activity of the antigen it binds. Certain blocking antibodies or antagonist antibodies substantially or completely inhibit the biological activity of the antigen.
  • An "agonist antibody,” as used herein, is an antibody which partially or fully mimics at least one of the functional activities of a polypeptide of interest.
  • “Growth inhibitory” antibodies are those that prevent or reduce proliferation of a cell expressing an antigen to which the antibody binds.
  • Antibody effector functions refer to those biological activities attributable to the Fc region (a native sequence Fc region or amino acid sequence variant Fc region) of an antibody, and vary with the antibody isotype. Examples of antibody effector functions include: Clq binding and complement dependent cytotoxicity (CDC); Fc receptor binding; antibody-dependent cell-mediated cytotoxicity (ADCC); phagocytosis; down regulation of cell surface receptors (e.g. B cell receptor); and B cell activation.
  • Fc region herein is used to define a C-terminal region of an immunoglobulin heavy chain, including native sequence Fc regions and variant Fc regions.
  • the human IgG heavy chain Fc region is usually defined to stretch from an amino acid residue at position Cys226, or from Pro230, to the carboxyl- terminus thereof.
  • the C-terminal lysine (residue 447 according to the EU numbering system) of the Fc region may be removed, for example, during production or purification of the antibody, or by recombinantly engineering the nucleic acid encoding a heavy chain of the antibody. Accordingly, a composition of intact antibodies may comprise antibody populations with all K447 residues removed, antibody populations with no K447 residues removed, and antibody populations having a mixture of antibodies with and without the K447 residue.
  • a "functional Fc region” possesses an "effector function” of a native sequence Fc region.
  • effector functions include Clq binding; CDC; Fc receptor binding; ADCC; phagocytosis; down regulation of cell surface receptors (e.g. B cell receptor; BCR), etc.
  • Such effector functions generally require the Fc region to be combined with a binding domain (e.g., an antibody variable domain) and can be assessed using various assays as disclosed, for example, in definitions herein.
  • a "native sequence Fc region” comprises an amino acid sequence identical to the amino acid sequence of an Fc region found in nature.
  • Native sequence human Fc regions include a native sequence human IgGl Fc region (non-A and A allotypes); native sequence human IgG2 Fc region; native sequence human IgG3 Fc region; and native sequence human IgG4 Fc region as well as naturally occurring variants thereof.
  • a “variant Fc region” comprises an amino acid sequence which differs from that of a native sequence Fc region by virtue of at least one amino acid modification, preferably one or more amino acid substitution(s).
  • the variant Fc region has at least one amino acid substitution compared to a native sequence Fc region or to the Fc region of a parent polypeptide, e.g. from about one to about ten amino acid substitutions, and preferably from about one to about five amino acid substitutions in a native sequence Fc region or in the Fc region of the parent polypeptide.
  • the variant Fc region herein will preferably possess at least about 80% homology with a native sequence Fc region and/or with an Fc region of a parent polypeptide, and most preferably at least about 90% homology therewith, more preferably at least about 95% homology therewith.
  • Fc receptor or “FcR” describes a receptor that binds to the Fc region of an antibody.
  • an FcR is a native human FcR.
  • an FcR is one which binds an IgG antibody (a gamma receptor) and includes receptors of the FcyRI, FcyRII, and FcyRIII subclasses, including allelic variants and alternatively spliced forms of those receptors.
  • FcyRII receptors include FcyRIIA (an "activating receptor") and FcyRIIB (an “inhibiting receptor”), which have similar amino acid sequences that differ primarily in the cytoplasmic domains thereof.
  • Activating receptor FcyRIIA contains an immunoreceptor tyrosine -based activation motif (ITAM) in its cytoplasmic domain.
  • Inhibiting receptor FcyRIIB contains an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic domain, (see, e.g., Daeron, Annu. Rev. Immunol. 15:203-234 (1997)).
  • FcRs are reviewed, for example, in Ravetch and Kinet, Annu. Rev. Immunol 9:457-92 (1991); Capel et al, Immunomethods 4:25-34 (1994); and de Haas et al, J. Lab. Clin. Med. 126:330-41 (1995).
  • Other FcRs including those to be identified in the future, are encompassed by the term "FcR" herein.
  • Fc receptor or “FcR” also includes the neonatal receptor, FcRn, which is responsible for the transfer of maternal IgGs to the fetus (Guyer et al, J. Immunol. 117:587 (1976) and Kim et al, J. Immunol. 24:249 (1994)) and regulation of homeostasis of immunoglobulins. Methods of measuring binding to FcRn are known (see, e.g., Ghetie and Ward., Immunol. Today 18(12):592-598 (1997); Ghetie et al, Nature Biotechnology, 15(7):637-640 (1997); Hinton et al, J. Biol. Chem. 279(8):6213-6216 (2004); WO 2004/92219 (Hinton et al).
  • Binding to human FcRn in vivo and serum half life of human FcRn high affinity binding polypeptides can be assayed, e.g., in transgenic mice or transfected human cell lines expressing human FcRn, or in primates to which the polypeptides with a variant Fc region are administered.
  • WO 2000/42072 (Presta) describes antibody variants with improved or diminished binding to FcRs. See also, e.g., Shields et al. J. Biol. Chem. 9(2):6591-6604 (2001).
  • Human effector cells are leukocytes which express one or more FcRs and perform effector functions.
  • the cells express at least FcyRIII and perform ADCC effector function(s).
  • human leukocytes which mediate ADCC include peripheral blood mononuclear cells (PBMC), natural killer (NK) cells, monocytes, cytotoxic T cells, and neutrophils.
  • PBMC peripheral blood mononuclear cells
  • NK natural killer
  • monocytes monocytes
  • cytotoxic T cells cytotoxic T cells
  • neutrophils neutrophils.
  • the effector cells may be isolated from a native source, e.g., from blood.
  • ADCC antibody-dependent cell-mediated cytotoxicity
  • cytotoxic cells e.g. NK cells, neutrophils, and macrophages
  • NK cells e.g. NK cells, neutrophils, and macrophages
  • FcRs Fc receptors
  • FcR expression on hematopoietic cells is summarized in Table 3 on page 464 of Ravetch and Kinet, Annu. Rev.
  • ADCC activity of a molecule of interest may be assessed in vitro, such as that described in US Patent No. 5,500,362 or 5,821,337 or U.S. Patent No. 6,737,056 (Presta).
  • Useful effector cells for such assays include PBMC and NK cells.
  • ADCC activity of the molecule of interest may be assessed in vivo, e.g. , in an animal model such as that disclosed in Clynes et al. PNAS (USA) 95:652-656 (1998).
  • Complement dependent cytotoxicity or “CDC” refers to the lysis of a target cell in the presence of complement. Activation of the classical
  • complement pathway is initiated by the binding of the first component of the complement system (Clq) to antibodies (of the appropriate subclass), which are bound to their cognate antigen.
  • Clq complement system
  • a CDC assay e.g., as described in Gazzano-Santoro et al., J. Immunol. Methods 202: 163 (1996), may be performed.
  • Polypeptide variants with altered Fc region amino acid sequences polypeptides with a variant Fc region
  • increased or decreased Clq binding capability are described, e.g., in US Patent No. 6,194,551 Bl and WO 1999/51642. See also, e.g., Idusogie et al. J. Immunol. 164: 4178-4184 (2000).
  • Fc region-comprising antibody refers to an antibody that comprises an Fc region.
  • the C-terminal lysine (residue 447 according to the EU numbering system) of the Fc region may be removed, for example, during purification of the antibody or by recombinant engineering of the nucleic acid encoding the antibody.
  • a composition comprising an antibody having an Fc region according to this invention can comprise an antibody with K447, with all K447 removed, or a mixture of antibodies with and without the K447 residue.
  • Binding affinity generally refers to the strength of the sum total of noncovalent interactions between a single binding site of a molecule (e.g. , an antibody) and its binding partner (e.g., an antigen). Unless indicated otherwise, as used herein, “binding affinity” refers to intrinsic binding affinity which reflects a 1 : 1 interaction between members of a binding pair (e.g., antibody and antigen).
  • the affinity of a molecule X for its partner Y can generally be represented by the dissociation constant (Kd). Affinity can be measured by common methods known in the art, including those described herein.
  • Low-affinity antibodies generally bind antigen slowly and tend to dissociate readily, whereas high-affinity antibodies generally bind antigen faster and tend to remain bound longer.
  • a variety of methods of measuring binding affinity are known in the art, any of which can be used for purposes of the present invention. Specific illustrative and exemplary embodiments for measuring binding affinity are described in the following.
  • the "Kd" or "Kd value” according to this invention is measured by a radiolabeled antigen binding assay (RIA) performed with the Fab version of an antibody of interest and its antigen as described by the following assay.
  • RIA radiolabeled antigen binding assay
  • Solution binding affinity of Fabs for antigen is measured by equilibrating Fab with a minimal concentration of ( 125 I)-labeled antigen in the presence of a titration series of unlabeled antigen, then capturing bound antigen with an anti-Fab antibody- coated plate (see, e.g., Chen, et al., J. Mol. Biol. 293:865-881(1999)).
  • MICROTITER ® multi-well plates (Thermo Scientific) are coated overnight with 5 ⁇ g/ml of a capturing anti-Fab antibody (Cappel Labs) in 50 mM sodium carbonate (pH 9.6), and subsequently blocked with 2% (w/v) bovine serum albumin in PBS for two to five hours at room temperature (approximately 23°C).
  • a non-adsorbent plate (Nunc #269620)
  • 100 pM or 26 pM [ 125 I] -antigen are mixed with serial dilutions of a Fab of interest (e.g. , consistent with assessment of the anti-VEGF antibody, Fab-12, in Presta et al, Cancer Res. 57:4593-4599 (1997)).
  • the Fab of interest is then incubated overnight; however, the incubation may continue for a longer period (e.g., about 65 hours) to ensure that equilibrium is reached.
  • the Kd or Kd value is measured by using surface plasmon resonance assays using a BIACORE ® -2000 or a BIACORE ® -3000 (BIAcore, Inc., Piscataway, NJ) at 25°C with immobilized antigen CM5 chips at -10 response units (RU).
  • CM5 carboxymethylated dextran biosensor chips
  • EDC N-ethyl-N'- (3-dimethylaminopropyl)- carbodiimide hydrochloride
  • NHS N-hydroxysuccinimide
  • BIACORE ® -2000 or a BIACORE ® -3000 system (BIAcore, Inc., Piscataway, NJ).
  • the difference between said two values is, for example, less than about 50%, less than about 40%>, less than about 30%>, less than about 20%, and/or less than about 10% as a function of the reference/comparator value.
  • the phrase "substantially reduced,” or “substantially different,” as used herein, denotes a sufficiently high degree of difference between two numeric values (generally one associated with a molecule and the other associated with a reference/comparator molecule) such that one of skill in the art would consider the difference between the two values to be of statistical significance within the context of the biological characteristic measured by said values (e.g., Kd values).
  • the difference between said two values is, for example, greater than about 10%>, greater than about 20%, greater than about 30%, greater than about 40%, and/or greater than about 50% as a function of the value for the reference/comparator molecule.
  • an "acceptor human framework” for the purposes herein is a framework comprising the amino acid sequence of a VL or VH framework derived from a human immunoglobulin framework or a human consensus framework.
  • An acceptor human framework "derived from” a human immunoglobulin framework or a human consensus framework may comprise the same amino acid sequence thereof, or it may contain pre-existing amino acid sequence changes. In some embodiments, the number of pre-existing amino acid changes are 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, or 2 or less.
  • VH preferably those changes occur at only three, two, or one of positions 71H, 73H and 78H; for instance, the amino acid residues at those positions may be 71 A, 73T and/or 78A.
  • the VL acceptor human framework is identical in sequence to the VL human immunoglobulin framework sequence or human consensus framework sequence.
  • a "human consensus framework” is a framework which represents the most commonly occurring amino acid residues in a selection of human immunoglobulin VL or VH framework sequences.
  • the selection of human immunoglobulin VL or VH sequences is from a subgroup of variable domain sequences.
  • the subgroup of sequences is a subgroup as in Kabat et al, supra.
  • the subgroup is subgroup kappa I as in Kabat et al, supra.
  • the subgroup III is subgroup III as in Kabat et al., supra.
  • VH subgroup III consensus framework comprises the consensus sequence obtained from the amino acid sequences in variable heavy subgroup III of Kabat et al.
  • the VH subgroup III consensus framework amino acid sequence comprises at least a portion or all of each of the following sequences: EVQLVESGGGLVQPGGSLRLSCAAS (SEQ ID N0:63)-H1- WVRQAPGKGLEWV (SEQ ID NO:64)-H2-
  • VL subgroup I consensus framework comprises the consensus sequence obtained from the amino acid sequences in variable light kappa subgroup I of Kabat et al.
  • the VH subgroup I consensus framework amino acid sequence comprises at least a portion or all of each of the following sequences: DIQMTQSPSSLSASVGDRVTITC (SEQ ID NO : 67)-L 1 -WYQQKPGKAPKLLIY (SEQ ID NO:68)-L2-GVPSPvFSGSGSGTDFTLTISSLQPEDFATYYC (SEQ ID NO:69)-L3-FGQGTKVEIK (SEQ ID NO:70). See Figure 5.
  • cognate set refers to a set of different nucleotide triplet sequences used to encode desired variant amino acids.
  • oligonucleotides can be synthesized, for example, by solid phase synthesis, including sequences that represent all possible combinations of nucleotide triplets provided by the codon set and that will encode the desired group of amino acids.
  • a standard form of codon designation is that of the IUB code, which is known in the art and described herein.
  • a codon set typically is represented by 3 capital letters in italics, e.g., NNK, NNS, XYZ, DVK and the like.
  • a "non-random codon set" thus refers to a codon set that encodes select amino acids that fulfill partially, preferably completely, the criteria for amino acid selection as described herein.
  • oligonucleotides with selected nucleotide "degeneracy" at certain positions is well known in that art, for example the TRIM approach (Knappek et al. (1999) J. Mol. Biol. 296:57-86); Garrard & Henner (1993) Gene 128: 103).
  • Such sets of oligonucleotides having certain codon sets can be synthesized using commercial nucleic acid synthesizers (available from, for example, Applied Biosystems, Foster City, CA), or can be obtained commercially (for example, from Life Technologies, Rockville, MD).
  • a set of oligonucleotides synthesized having a particular codon set will typically include a plurality of oligonucleotides with different sequences, the differences established by the codon set within the overall sequence.
  • Oligonucleotides, as used according to the invention have sequences that allow for hybridization to a variable domain nucleic acid template and also can, but does not necessarily, include restriction enzyme sites useful for, for example, cloning purposes.
  • linear antibodies refers to the antibodies described in Zapata et al. (1995 Protein Eng, 8(10): 1057-1062). Briefly, these antibodies comprise a pair of tandem Fd segments (V H -C h 1-V H -C h 1) which, together with complementary light chain polypeptides, form a pair of antigen binding regions. Linear antibodies can be bispecific or monospecific.
  • library refers to a plurality of antibody or antibody fragment sequences (for example, polypeptides of the invention), or the nucleic acids that encode these sequences, the sequences being different in the combination of variant amino acids that are introduced into these sequences according to the methods of the invention.
  • Phage display is a technique by which variant polypeptides are displayed as fusion proteins to at least a portion of coat protein on the surface of phage, e.g., filamentous phage, particles.
  • a utility of phage display lies in the fact that large libraries of randomized protein variants can be rapidly and efficiently sorted for those sequences that bind to a target antigen with high affinity. Display of peptide and protein libraries on phage has been used for screening millions of polypeptides for ones with specific binding properties. Polyvalent phage display methods have been used for displaying small random peptides and small proteins through fusions to either gene III or gene VIII of filamentous phage. Wells and Lowman (1992) Curr. Opin. Struct.
  • a protein or peptide library is fused to a gene III or a portion thereof, and expressed at low levels in the presence of wild type gene III protein so that phage particles display one copy or none of the fusion proteins.
  • Avidity effects are reduced relative to polyvalent phage so that sorting is on the basis of intrinsic ligand affinity, and phagemid vectors are used, which simplify DNA manipulations. Lowman and Wells (1991) Methods: A companion to Methods in Enzymology 3:205-0216.
  • a "phagemid” is a plasmid vector having a bacterial origin of replication, e.g., ColEl, and a copy of an intergenic region of a bacteriophage.
  • the phagemid may be used on any known bacteriophage, including filamentous bacteriophage and lambdoid bacteriophage.
  • the plasmid will also generally contain a selectable marker for antibiotic resistance. Segments of DNA cloned into these vectors can be propagated as plasmids. When cells harboring these vectors are provided with all genes necessary for the production of phage particles, the mode of replication of the plasmid changes to rolling circle replication to generate copies of one strand of the plasmid DNA and package phage particles.
  • the phagemid may form infectious or non-infectious phage particles.
  • This term includes phagemids which contain a phage coat protein gene or fragment thereof linked to a heterologous polypeptide gene as a gene fusion such that the heterologous polypeptide is displayed on the surface of the phage particle.
  • phage vector means a double stranded replicative form of a bacteriophage containing a heterologous gene and capable of replication.
  • the phage vector has a phage origin of replication allowing phage replication and phage particle formation.
  • the phage is preferably a filamentous bacteriophage, such as an Ml 3, fl, fd, Pf3 phage or a derivative thereof, or a lambdoid phage, such as lambda, 21, phi80, phi81, 82, 424, 434, etc., or a derivative thereof.
  • solvent accessible position refers to a position of an amino acid residue in the variable regions of the heavy and light chains of a source antibody or antigen binding fragment that is determined, based on structure, ensemble of structures and/or modeled structure of the antibody or antigen binding fragment, as potentially available for solvent access and/or contact with a molecule, such as an antibody-specific antigen. These positions are typically found in the CDRs and on the exterior of the protein.
  • the solvent accessible positions of an antibody or antigen binding fragment, as defined herein, can be determined using any of a number of algorithms known in the art.
  • solvent accessible positions are determined using coordinates from a 3 -dimensional model of an antibody, preferably using a computer program such as the Insightll program (Accelrys, San Diego, CA). Solvent accessible positions can also be determined using algorithms known in the art (e.g., Lee and Richards (1971) J. Mol. Biol. 55, 379 and Connolly (1983) J. Appl. Cryst. 16, 548). Determination of solvent accessible positions can be performed using software suitable for protein modeling and 3 -dimensional structural information obtained from an antibody. Software that can be utilized for these purposes includes SYBYL Biopolymer Module software (Tripos Associates).
  • vascular endothelial growth factor has been identified as the key factor involved in stimulating angiogenesis and in inducing vascular permeability. Ferrara et al, Endocr. Rev. 18:4-25 (1997). The finding that the loss of even a single VEGF allele results in embryonic lethality points to an irreplaceable role played by this factor in the development and differentiation of the vascular system. Furthermore, VEGF has been shown to be a key mediator of neovascularization associated with tumors and intraocular disorders.
  • Anti-VEGF neutralizing antibodies suppress the growth of a variety of human tumor cell lines in nude mice (Kim et al, Nature 362:841-844 (1993); Warren et al, J. Clin. Invest. 95 : 1789-1797 (1995); Borgstrom et al, Cancer Res. 56:4032-4039 (1996); Melnyk et al, Cancer Res. 56:921-924 (1996)) and also inhibit intraocular angiogenesis in models of ischemic retinal disorders. Adamis et al, Arch. Ophthalmol. 1 14:66-71 (1996).
  • anti- VEGF monoclonal antibodies or other inhibitors of VEGF action are promising candidates for the treatment of tumors and various intraocular neo vascular disorders.
  • Such antibodies are described, for example, in EP 817,648 published January 14, 1998; and in
  • CRC metastatic colorectal cancer
  • NSCLC non-samll cell lung cancer
  • bevacizumab is being investigated in many ongoing clinical trials for treating various cancer indications.
  • anti-VEGF antibodies anti-Nrpl antibodies and anti-Nrp2 antibodies are also known, and described, for example, in Liang et al, J Mol Biol 366, 815-829 (2007) and Liang et al., J Biol Chem 281, 951-961 (2006), PCT publication number WO2007/056470 and PCT Application No. PCT/US2007/069179, the content of these patent applications are expressly incorporated herein by reference.
  • angiogenic factor or agent is a growth factor which stimulates the development of blood vessels, e.g., promote angiogenesis, endothelial cell growth, stabiliy of blood vessels, and/or vasculogenesis, etc.
  • angiogenic factors include, but are not limited to, e.g., VEGF and members of the VEGF family (VEGF- B, VEGF-C and VEGF-D), P1GF, PDGF family, fibroblast growth factor family (FGFs), TIE ligands (Angiopoietins), ephrins, Delta- like ligand 4 (DLL4), Del-1, fibroblast growth factors: acidic (aFGF) and basic (bFGF), Follistatin, Granulocyte colony-stimulating factor (G-CSF), Hepatocyte growth factor (HGF) /scatter factor (SF), Interleukin-8 (IL-8), Leptin, Midkine,neuropil
  • IGF-I insulin-like growth factor-I
  • VIGF insulin-like growth factor
  • EGF epidermal growth factor
  • CTGF tumor necrosis factor
  • TGF-alpha and TGF-beta TGF-beta
  • Oncogene 22:6549-6556 e.g., Table 1 listing known angiogenic factors
  • an "anti-angiogenesis agent” or “angiogenesis inhibitor” refers to a small molecular weight substance, a polynucleotide (including, e.g., an inhibitory RNA (RNAi or siRNA)), a polypeptide, an isolated protein, a recombinant protein, an antibody, or conjugates or fusion proteins thereof, that inhibits angiogenesis, vasculogenesis, or undesirable vascular permeability, either directly or indirectly.
  • RNAi or siRNA inhibitory RNA
  • the anti-angiogenesis agent includes those agents that bind and block the angiogenic activity of the angiogenic factor or its receptor.
  • an anti-angiogenesis agent is an antibody or other antagonist to an angiogenic agent as defined above, e.g., antibodies to VEGF-A or to the VEGF-A receptor (e.g., KDR receptor or Flt-1 receptor), anti-PDGFR inhibitors such as GleevecTM (Imatinib Mesylate), small molecules that block VEGF receptor signaling (e.g., PTK787/ZK2284, SU6668, SUTENT®/SU 11248 (sunitinib malate), AMG706, or those described in, e.g., international patent application WO 2004/113304).
  • an angiogenic agent as defined above, e.g., antibodies to VEGF-A or to the VEGF-A receptor (e.g., KDR receptor or Flt-1 receptor), anti-PDGFR inhibitors such as GleevecTM (Imatinib Mesylate), small molecules that block VEGF receptor signaling (e.g., PTK787
  • Anti- angiogensis agents also include native angiogenesis inhibitors , e.g., angiostatin, endostatin, etc. See, e.g., Klagsbrun and D'Amore (1991) Annu. Rev. Physiol.
  • Oncogene 22:3172-3179 e.g., Table 3 listing anti-angiogenic therapy in malignant melanoma
  • Tonini et al. (2003) Oncogene 22:6549-6556 e.g., Table 2 listing known antiangiogenic factors
  • Sato (2003) Int. J. Clin. Oncol. 8:200- 206 e.g., Table 1 listing anti-angiogenic agents used in clinical trials.
  • VEGF refers to the 165- amino acid human vascular endothelial cell growth factor and related 121-, 189-, and 206- amino acid human vascular endothelial cell growth factors, as described by Leung et al. (1989) Science 246: 1306, and Houck et al. (1991) Mol. Endocrin, 5: 1806, together with the naturally occurring allelic and processed forms thereof.
  • VEGF also refers to VEGFs from non-human species such as mouse, rat or primate. Sometimes the VEGF from a specific species are indicated by terms such as hVEGF for human VEGF, mVEGF for murine VEGF, and etc.
  • VEGF is also used to refer to truncated forms of the polypeptide comprising amino acids 8 to 109 or 1 to 109 of the 165 -amino acid human vascular endothelial cell growth factor. Reference to any such forms of VEGF may be identified in the present application, e.g., by "VEGF (8-109),” “VEGF (1-109)” or “VEGFi 65 .”
  • the amino acid positions for a "truncated" native VEGF are numbered as indicated in the native VEGF sequence. For example, amino acid position 17 (methionine) in truncated native VEGF is also position 17 (methionine) in native VEGF.
  • the truncated native VEGF has binding affinity for the KDR and Flt-1 receptors comparable to native VEGF.
  • a "VEGF antagonist” refers to a molecule capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with VEGF activities including, but not limited to, its binding to one or more VEGF receptors.
  • VEGF antagonists include, without limitation, anti-VEGF antibodies and antigen-binding fragments thereof, receptor molecules and derivatives which bind specifically to VEGF thereby sequestering its binding to one or more receptors, anti-VEGF receptor antibodies and VEGF receptor antagonists such as small molecule inhibitors of the VEGFR tyrosine kinases.
  • VEGF antagonist specifically includes molecules, including antibodies, antibody fragments, other binding polypeptides, peptides, and non-peptide small molecules, that bind to VEGF and are capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with VEGF activities.
  • VEGF activities specifically includes VEGF mediated biological activities of VEGF.
  • an "anti-VEGF antibody” is an antibody that binds to VEGF with sufficient affinity and specificity.
  • the anti-VEGF antibody can be used as a therapeutic agent in targeting and interfering with diseases or conditions wherein the VEGF activity is involved.
  • An anti-VEGF antibody will usually not bind to other VEGF homologues such as VEGF-B or VEGF-C, nor other growth factors such as P1GF, PDGF or bFGF.
  • anti-VEGF antibodies include a monoclonal antibody that binds to the same epitope as the monoclonal anti-VEGF antibody A4.6.1 produced by hybridoma ATCC HB 10709; a recombinant humanized anti-VEGF monoclonal antibody (see Presta et al. (1997) Cancer Res. 57:4593-4599), including but not limited to the antibody known as "bevacizumab (BV),” also known as “rhuMAb VEGF” or "AVASTIN ® .”
  • BV bevacizumab
  • AVASTIN ® presently commercially available.
  • Bevacizumab comprises mutated human IgGi framework regions and antigen-binding complementarity-determining regions from the murine antibody A.4.6.1 that blocks binding of human VEGF to its receptors. Approximately 93% of the amino acid sequence of bevacizumab, including most of the framework regions, is derived from human IgGi, and about 7% of the sequence is derived from A4.6.1. Bevacizumab has a molecular mass of about 149,000 daltons and is glycosylated. Bevacizumab and other humanized anti-VEGF antibodies are further described in U.S. Pat. No. 6,884,879, issued February 26, 2005.
  • Additional anti-VEGF antibodies include the G6 or B20 series antibodies ⁇ e.g., G6-23, G6-31, B20-4.1), as described in PCT Application Publication No. WO2005/012359.
  • G6 or B20 series antibodies ⁇ e.g., G6-23, G6-31, B20-4.1
  • For additional antibodies see U.S. Pat. Nos. 7,060,269, 6,582,959, 6,703,020; 6,054,297; W098/45332; WO 96/30046; WO94/10202; EP 0666868B1; U.S. Patent Application Publication Nos. 2006009360, 20050186208, 20030206899, 20030190317, 20030203409, and
  • B20 series polypeptide refers to a polypeptide, including an antibody that binds to VEGF.
  • B20 series polypeptides includes, but not limited to, antibodies derived from a sequence of the B20 antibody or a B20-derived antibody described in US Publication No. 20060280747, US Publication No. 20070141065 and/or US Publication No. 20070020267, the content of these patent applications are expressly incorporated herein by reference.
  • B20 series polypeptide is B20-4.1 as described in US Publication No. 20060280747, US Publication No. 20070141065 and/or US Publication No.
  • B20 series polypeptide is B20-4.1.1 described in PCT Publication No. WO 2009/073160, the entire disclosure of which is expressly incorporated herein by reference.
  • G6 series polypeptide refers to a polypeptide, including an antibody that binds to VEGF.
  • G6 series polypeptides includes, but not limited to, antibodies derived from a sequence of the G6 antibody or a G6-derived antibody described in US Publication No. 20060280747, US Publication No. 20070141065 and/or US Publication No. 20070020267.
  • G6 series polypeptides, as described in US Publication No. 20060280747, US Publication No. 20070141065 and/or US Publication No. 20070020267 include, but not limited to, G6-8, G6-23 and G6-31.
  • VEGF-C a member of the VEGF family, is known to bind at least two cell surface receptor families, the tyrosine kinase VEGF receptors and the neuropilin (Nrp) receptors.
  • VEGF-C can bind VEGFR2 (KDPv receptor) and VEGFR3 (Flt-4 receptor) leading to receptor dimerization (Shinkai et al, J Biol Chem 273, 31283-31288 (1998)), kinase activation and autophosphorylation (Heldin, Cell 80, 213-223 (1995); Waltenberger et al, J. Biol Chem 269, 26988-26995 (1994)).
  • the phosphorylated receptor induces the activation of multiple substrates leading to angiogenesis and lymphangiogenesis (Ferrara et al, Nat Med 9, 669-676 (2003)).
  • VEGF-C is one of the best studied mediators of lymphatic development. Overexpression of VEGF-C in tumor cells was shown to promote tumor-associated lymphangiogenesis, resulting in enhanced metastasis to regional lymph nodes (Karpanen et al., Faseb J 20, 1462-1472 (2001); Mandriota et al, EMBO J 20, 672-682 (2001); Skobe et al, Nat Med ⁇ , 192-198 (2001); Stacker et al, Nat Rev Cancer 2, 573-583 (2002); Stacker et al., Faseb J 16, 922-934 (2002)).
  • VEGF-C expression has also been correlated with tumor-associated lymphangiogenesis and lymph node metastasis for a number of human cancers (reviewed in Achen et al., 2006, supra.
  • blockade of VEGF-C-mediated signaling has been shown to suppress tumor lymphangiogenesis and lymph node metastases in mice (Chen et al, Cancer Res 65, 9004-9011 (2005); He et al, J. Natl Cancer Inst 94, 8190825 (2002); Krishnan et al, Cancer Res 63, 713-722 (2003); Lin et al., Cancer Res 65, 6901-6909 (2005)).
  • vascular endothelial growth factor-C vascular endothelial growth factor-C
  • VEGF-C vascular endothelial growth factor-C
  • VRP vascular endothelial growth factor 2
  • VEGF-2 vascular endothelial growth factor-2
  • active fragments include any portions of the full-length amino acid sequence which have less than the full 419 amino acids of the full-length amino acid sequence as shown in SEQ ID NO: 87.
  • Such active fragments contain VEGF-C biological activity and include, but not limited to, mature VEGF-C.
  • the full-length VEGF-C the full-length VEGF-C
  • VEGF-C polypeptide is proteolytically processed produce a mature form of VEGF-C polypeptide, also referred to as mature VEGF-C.
  • processing includes cleavage of a signal peptide and cleavage of an amino-terminal peptide (corresponding approximately to amino acids 1-102 of SEQ ID NO: 87) and cleavage of a carboxyl- terminal peptide (corresponding approximately to amino acids 228-419 of SEQ ID NO:87) to produced a fully-processed mature form ⁇ see Fig. 6).
  • Experimental evidence demonstrates that the full-length VEGF-C, partially-processed forms of VEGF-C and fully processed mature forms of VEGF-C are able to bind VEGFR3 (Flt-4 receptor). However, high affinity binding to VEGFR2 occurs only with the fully processed mature forms of VEGF-C.
  • VEGF-C also refers to VEGF-C from non-human species such as mouse, rat or primate. Sometimes the VEGF-C from a specific species are indicated by terms such as hVEGF-C for human VEGF-C, mVEGF-C for murine VEGF-C, and etc.
  • C137S vascular endothelial growth factor-C C137S
  • VEGFC C137S vascular endothelial growth factor-C C137S
  • h VEGF-C C137S refer to the full-length polypeptide and the fragments of the full-length polypeptide wherein the cystein residue at amino acid residue position 137 was replaced by a serine residue as shown in SEQ ID NO:88.
  • VEGF-C antagonist is used herein to refer to a molecule capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with VEGF-C activities.
  • VEGF-C antagonist refers to a molecule capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with the ability of VEGF-C to modulate angiogenesis, lymphatic endothelial cell (EC) migration, proliferation or adult lymphangiogenesis, especially tumoral lymphangiogenesis and tumor metastasis.
  • EC lymphatic endothelial cell
  • VEGF-C antagonists include, without limitation, anti- VEGF-C antibodies and antigen-binding fragments thereof, receptor molecules and derivatives which bind specifically to VEGF-C thereby sequestering its binding to one or more receptors, anti-VEGF-C receptor antibodies and VEGF-C receptor antagonists such as small molecule inhibitors of the VEGFR2 and VEGFR3.
  • VEGF-C antagonist specifically includes molecules, including antibodies, antibody fragments, other binding polypeptides, peptides, and non-peptide small molecules, that bind to VEGF-C and are capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with VEGF-C activities.
  • VEGF-C activities specifically includes VEGF-C mediated biological activities (as hereinabove defined) of VEGF-C.
  • anti-VEGF-C antibody or "an antibody that binds to VEGF-C” refers to an antibody that is capable of binding VEGF-C with sufficient affinity such that the antibody is useful as a diagnostic and/or therapeutic agent in targeting VEGF-C.
  • the extent of binding of an anti-VEGF-C antibody to an unrelated, non- VEGF-C protein is less than about 10% of the binding of the antibody to VEGF-C as measured, e.g., by a radioimmunoassay (RIA).
  • RIA radioimmunoassay
  • an antibody that binds to VEGF-C has a dissociation constant (Kd) of ⁇ ⁇ , ⁇ 100 nM, ⁇ 10 nM, ⁇ 1 nM, or ⁇ 0.1 nM.
  • Kd dissociation constant
  • an anti-VEGF-C antibody binds to an epitope of VEGF-C that is conserved among VEGF-C from different species.
  • VEGF-C biological activity
  • biological activity means having the ability to bind to, and stimulate the phosphorylation of, the Flt-4 receptor (VEGFR3).
  • Flt-4 receptor VAGFR3
  • VEGF-C will bind to the extracellular domain of the Flt-4 receptor and thereby activate or inhibit the intracellular tyrosine kinase domain thereof.
  • binding of VEGF-C to the receptor may result in enhancement or inhibition of proliferation and/or differentiation and/or activation of cells having the Flt-4 receptor for the VEGF-C in vivo or in vitro.
  • Binding of VEGF-C to the Flt-4 receptor can be determined using conventional techniques, including competitive binding methods, such as RIAs, ELISAs, and other competitive binding assays.
  • Ligand/receptor complexes can be identified using such separation methods as filtration,
  • VEGF-C induces phosphorylation of the Flt- 4 receptor
  • conventional tyrosine phosphorylation assays can also be used as an indication of the formation of a Flt-4 receptor/VEGF-C complex.
  • VEGF-C "biological activity" means having the ability to bind to KDR receptor (VEGFR2).
  • vascular permeability, as well as the migration and proliferation of endothelial cells binding of VEGF-C to the KDR receptor may result in enhancement or inhibition of vascular permeability as well as migration and/or proliferation and/or differentiation and/or activation of endothelial cells having the KDR receptor for the VEGF-C in vivo or in vitro.
  • treatment refers to clinical intervention in an attempt to alter the natural course of the individual or cell being treated, and can be performed either for prophylaxis or during the course of clinical pathology. Desirable effects of treatment include preventing occurrence or recurrence of disease, alleviation of symptoms, diminishment of any direct or indirect pathological consequences of the disease, preventing metastasis, decreasing the rate of disease progression, amelioration or palliation of the disease state, and remission or improved prognosis.
  • antibodies of the invention are used to delay development of a disease or disorder or to slow the progression of a disease or disorder.
  • “Chronic” administration refers to administration of the agent(s) in a continuous mode as opposed to an acute mode, so as to maintain the initial therapeutic effect (activity) for an extended period of time. "Intermittent"
  • administration is treatment that is not consecutively done without interruption, but rather is cyclic in nature.
  • a "disorder” is any condition that would benefit from treatment.
  • This includes chronic and acute disorders or diseases including those pathological conditions which predispose the mammal to the disorder in question.
  • disorders to be treated herein include malignant and benign tumors; non-leukemias and lymphoid malignancies; and, in particular, tumor (cancer) metastasis.
  • cell proliferative disorder and “proliferative disorder” refer to disorders that are associated with some degree of abnormal cell proliferation.
  • the cell proliferative disorder is cancer.
  • Tumor refers to all neoplastic cell growth and proliferation, whether malignant or benign, and all pre-cancerous and cancerous cells and tissues.
  • cancer refers to all neoplastic cell growth and proliferation, whether malignant or benign, and all pre-cancerous and cancerous cells and tissues.
  • proliferative disorder and “tumor” are not mutually exclusive as referred to herein.
  • cancer and “cancerous” refer to or describe the physiological condition in mammals that is typically characterized by unregulated cell growth.
  • examples of cancer include but are not limited to, carcinoma, lymphoma, blastoma, sarcoma, and leukemia or lymphoid malignancies.
  • cancers include, but not limited to, squamous cell cancer (e.g., epithelial squamous cell cancer), lung cancer including small-cell lung cancer, non- small cell lung cancer, adenocarcinoma of the lung and squamous carcinoma of the lung, cancer of the peritoneum, hepatocellular cancer, gastric or stomach cancer including gastrointestinal cancer and gastrointestinal stromal cancer, pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, cancer of the urinary tract, hepatoma, breast cancer, colon cancer, rectal cancer, colorectal cancer, endometrial or uterine carcinoma, salivary gland carcinoma, kidney or renal cancer, prostate cancer, vulval cancer, thyroid cancer, hepatic carcinoma, anal carcinoma, penile carcinoma, melanoma, superficial spreading melanoma, lentigo maligna melanoma, acral lentiginous melanomas, nodular melan
  • Macroglobulinemia may be chronic lymphocytic leukemia (CLL); acute lymphoblastic leukemia (ALL); hairy cell leukemia; chronic myeloblastic leukemia; and post- transplant lymphoproliferative disorder (PTLD), as well as abnormal vascular proliferation associated with phakomatoses, edema (such as that associated with brain tumors), Meigs' syndrome, brain, as well as head and neck cancer, and associated metastases.
  • CLL chronic lymphocytic leukemia
  • ALL acute lymphoblastic leukemia
  • PTLD post- transplant lymphoproliferative disorder
  • cancers that are amenable to treatment by the antibodies of the invention include breast cancer, colorectal cancer, rectal cancer, non- small cell lung cancer, glioblastoma, non-Hodgkins lymphoma (NHL), renal cell cancer, prostate cancer, liver cancer, pancreatic cancer, soft-tissue sarcoma, kaposi's sarcoma, carcinoid carcinoma, head and neck cancer, ovarian cancer, mesothelioma, and multiple myeloma.
  • breast cancer colorectal cancer, rectal cancer, non- small cell lung cancer, glioblastoma, non-Hodgkins lymphoma (NHL), renal cell cancer, prostate cancer, liver cancer, pancreatic cancer, soft-tissue sarcoma, kaposi's sarcoma, carcinoid carcinoma, head and neck cancer, ovarian cancer, mesothelioma, and multiple myeloma.
  • the cancer is selected from the group consisting of small cell lung cancer, glioblastoma, neuroblastomas, melanoma, breast carcinoma, gastric cancer, colorectal cancer (CRC), and hepatocellular carcinoma. Yet, in some embodiments, the cancer is selected from the group consisting of non- small cell lung cancer, colorectal cancer, renal cancer, ovarian cancer, glioblastoma and breast carcinoma, including metastatic forms of those cancers.
  • cancer is meant the spread of cancer from its primary site to other places in the body. Cancer cells can break away from a primary tumor, penetrate into lymphatic and blood vessels, circulate through the bloodstream, and grow in a distant focus (metastasize) in normal tissues elsewhere in the body.
  • Metastasis can be local or distant. Metastasis is a sequential process, contingent on tumor cells breaking off from the primary tumor, traveling through the bloodstream or lymphatics, and stopping at a distant site. At the new site, the cells establish a blood supply and can grow to form a life-threatening mass.
  • the term metastatic tumor refers to a tumor that is capable of metastasizing, but has not yet metastasized to tissues or organs elsewhere in the body. In certain embodiments, the term metastatic tumor refers to a tumor that has metastasized to tissues or organs elsewhere in the body.
  • metastatic organ or “metastatic tissue” is used in the broadest sense, refers to an organ or a tissue in which the cancer cells from a primary tumor or the cancer cells from another part of the body have spread.
  • metastatic organ and metastatic tissue include, but not limited to, lung, liver, brain, ovary, bone and bone marrow.
  • pre-metastatic organ or "pre-metastatic tissue” as used herein, refers to an organ or a tissue in which no cancer cells from a primary tumor or from another part of the body have been detected.
  • the pre- metastatic organ or pre-metastatic tissue refers to an organ or tissue that is in the phase before the spread of cancer cells from a primary tumor or from another part of the body to this organ or tissue have occurred. Examples of pre- metastatic organ or pre-metastatic tissue include, but not limited to, lung, liver, brain, ovary, bone and bone marrow.
  • primary tumor or “primary cancer” is meant the original cancer and not a metastatic lesion located in another tissue, organ, or location in the subject's body.
  • Cancer recurrence refers to a return of cancer following treatment, and includes return of cancer in the primary organ, as well as distant recurrence, where the cancer returns outside of the primary organ.
  • tumor burden is meant the number of cancer cells, the size of a tumor, or the amount of cancer in the body. Tumor burden is also referred to as tumor load.
  • tumor number is meant the number of tumors.
  • Refractory refers to the resistance or non-responsiveness of a disease or condition to a treatment (e.g., the number of neoplastic plasma cells increases even though treatment if given).
  • a treatment e.g., the number of neoplastic plasma cells increases even though treatment if given.
  • refractory refers a resistance or non-responsiveness to any previous treatment including, but not limited to, VEGF antagonist, anti-angiogenic agents and chemotherapy treatments.
  • the term “refractory” refers an intrinsically non-responsiveness of a disease or condition to any previous treatment comprising a VEGF antagonist, anti-angiogenic agents and/or chemotherapy treatments.
  • the VEGF antagonist is an anti-VEGF antibody.
  • Relapsed refers to the regression of the patient's illness back to its former diseased state, especially the return of symptoms following an apparent recovery or partial recovery.
  • relapsed state refers to the process of returning to or the return to illness before the previous treatment including, but not limited to, VEGF antagonist, anti-angiogenic agents and/or chemotherapy treatments.
  • relapsed state refers to the process of returning to or the return to illness after an initial strong response to a cancer therapy comprising a VEGF antagonist, anti-angiogenic agents and/or chemotherapy treatments.
  • the VEGF antagonist is an anti-VEGF antibody.
  • anti-cancer therapy refers to a therapy useful in treating cancer.
  • anti-cancer therapeutic agents include, but are limited to, e.g., chemotherapeutic agents, growth inhibitory agents, cytotoxic agents, agents used in radiation therapy, anti-angiogenic agents, apoptotic agents, anti-tubulin agents, and other agents to treat cancer, such as anti-HER-2 antibodies, anti-CD20 antibodies, an epidermal growth factor receptor (EGFR) antagonist (e.g., a tyrosine kinase inhibitor), HER 1 /EGFR inhibitor (e.g., erlotinib (TARCEVA ® ), platelet derived growth factor inhibitors (e.g., GLEEVEC ® (Imatinib Mesylate)), a COX-2 inhibitor (e.g., celecoxib), Erbitux ® (cetuximab, Imclone), interferons, cytokines, antagonists (e
  • EGFR epidermal growth factor receptor
  • an "individual,” “subject,” or “patient” is a vertebrate.
  • the vertebrate is a mammal.
  • Mammals include, but are not limited to, farm animals (such as cows), sport animals, pets (such as cats, dogs, and horses), primates, mice and rats.
  • a mammal is a human.
  • sample refers to a composition that is obtained or derived from a subject of interest that contains a cellular and/or other molecular entity that is to be characterized and/or identified, for example based on physical, biochemical, chemical and/or physiological
  • the definition encompasses blood and other liquid samples of biological origin and tissue samples such as a biopsy specimen or tissue cultures or cells derived therefrom.
  • the source of the tissue sample may be solid tissue as from a fresh, frozen and/or preserved organ or tissue sample or biopsy or aspirate; blood or any blood constituents; bodily fluids; and cells from any time in gestation or development of the subject or plasma.
  • the definition includes biological samples that have been manipulated in any way after their procurement, such as by treatment with reagents, solubilization, or enrichment for certain components, such as proteins or polynucleotides, or embedding in a semi-solid or solid matrix for sectioning purposes.
  • a "section" of a tissue sample is meant a single part or piece of a tissue sample, e.g. a thin slice of tissue or cells cut from a tissue sample.
  • Samples include, but not limited to, primary or cultured cells or cell lines, cell supernatants, cell lysates, platelets, serum, plasma, vitreous fluid, lymph fluid, synovial fluid, follicular fluid, seminal fluid, amniotic fluid, milk, whole blood, urine, cerebro-spinal fluid, saliva, sputum, tears, perspiration, mucus, tumor lysates, and tissue culture medium, as well as tissue extracts such as homogenized tissue, tumor tissue, and cellular extracts.
  • the sample is a clinical sample.
  • the sample is used in a diagnostic assay.
  • the sample is obtained from a pre-metastatic organ or a pre-metastatic tissue.
  • the sample is obtained from a primary or metastatic tumor. Tissue biopsy is often used to obtain a representative piece of tumor tissue.
  • tumor cells can be obtained indirectly in the form of tissues or fluids that are known or thought to contain the tumor cells of interest. For instance, samples of lung cancer lesions may be obtained by resection, bronchoscopy, fine needle aspiration, bronchial brushings, or from sputum, pleural fluid or blood.
  • a sample is obtained from a subject or patient prior to treatment with anti-VEGF-C antibodies. In certain embodimenst, a sample is obtained from a subject or patient after the treatment with anti-VEGF-C antibodies. In certain embodiments, a sample is obtained from a subject or patient prior to VEGF antagonist therapy. In certain embodiments, a sample is obtained from a subject or patient prior to anti-VEGF antibody therapy. In certain embodiments, a sample is obtained before a cancer has metastasized. In certain embodiments, a sample is obtained after a cancer has metastasized.
  • an “effective amount” refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired therapeutic or prophylactic result.
  • a "therapeutically effective amount" of a substance/molecule of the invention may vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of the substance/molecule, to elicit a desired response in the individual.
  • a therapeutically effective amount encompasses an amount in which any toxic or detrimental effects of the substance/molecule are outweighed by the therapeutically beneficial effects.
  • a “prophylactically effective amount” refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired prophylactic result. Typically, but not necessarily, since a prophylactic dose is used in subjects prior to or at an earlier stage of disease, the prophylactically effective amount would be less than the therapeutically effective amount.
  • efficacy is used herein in the broadest sense and refers to immunoglobuin's, antibody's or Fc fusion protein's ability to produce a desired effect. In certain embodiments, efficacy refers to the maximal observed effect of an immunoglobulin, antibody or Fc fusion protein at saturating levels. In certain embodiments, efficacy refers to the EC 50 of an immunoglobulin, antibody or Fc fusion protein. In certain embodiments, efficacy refers to the potency of an immunoglobulin, antibody or Fc fusion protein.
  • efficacy refers to immunoglobulin's, antibody's or Fc fusion protein's ability to produce beneficial effects on the course or duration of a disease, including clinical benefit as defined herein.
  • EC 50 refers to the concentration of an immunoglobulin, antibody or Fc fusion protein which induces a response halfway between the baseline and maximum. In certain embodiments, EC 50 represents the concentration of an immunoglobulin, antibody or Fc fusion protein where 50% of its maximal effect is observed. In certain embodiments, EC 50 represents the plasma or serum concentration required for obtaining 50% of the maximum effect in vivo.
  • Efficacy in treating cancer may be demonstrated by detecting the ability of an antibody, a fusion protein, a conjugated molecule, or a composition of the invention to inhibit or reduce the growth or metastasis of cancerous cells or to ameliorate or alleviate one or more symptoms associated with cancer.
  • the treatment is considered therapeutic if there is, for example, a reduction in the growth or metastasis of cancerous cells, amelioration of one or more symptoms associated with cancer, or a decrease in mortality and/or morbidity following administration of an antibody, a fusion protein, a conjugated molecule, or a composition of the invention.
  • Antibodies, fusion proteins or compositions of the invention can be tested for their ability to reduce tumor formation in in vitro, ex vivo, and in vivo assays.
  • efficacy in vivo can, for example, be also measured by assessing the duration of survival, time to disease progression (TTP), the response rates (RR), duration of response, and/or quality of life.
  • Clinical benefit can be measured by assessing various endpoints, e.g., inhibition, to some extent, of disease progression, including slowing down and complete arrest; reduction in the number of disease episodes and/or symptoms;
  • maintenance therapy is meant a therapeutic regimen that is given to reduce the likelihood of disease recurrence or progression.
  • Maintenance therapy can be provided for any length of time, including extended time periods up to the life-span of the subject. Maintenance therapy can be provided after initial therapy or in conjunction with initial or additional therapies. Dosages used for maintenance therapy can vary and can include diminished dosages as compared to dosages used for other types of therapy.
  • adjuvant therapy refers to therapy given after surgery, where no evidence of residual disease can be detected, so as to reduce the risk of disease recurrence.
  • the goal of adjuvant therapy is to prevent recurrence of the cancer, and therefore to reduce the chance of cancer-related death.
  • Administration "in combination with” one or more further therapeutic agents includes simultaneous (concurrent) and consecutive administration in any order.
  • concurrent administration includes a dosing regimen when the administration of one or more agent(s) continues after discontinuing the administration of one or more other agent(s).
  • composition refers to a preparation which is in such form as to permit the biological activity of the active ingredient to be effective, and which contains no additional components which are unacceptably toxic to a subject to which the formulation would be administered.
  • Such formulations may be sterile.
  • a "sterile" formulation is aseptic or free from all living microorganisms and their spores.
  • Carriers as used herein include pharmaceutically acceptable carriers, excipients, or stabilizers which are nontoxic to the cell or mammal being exposed thereto at the dosages and concentrations employed. Often the
  • physiologically acceptable carrier is an aqueous pH buffered solution.
  • physiologically acceptable carriers include buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid; low molecular weight (less than about 10 residues) polypeptide; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, arginine or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugar alcohols such as mannitol or sorbitol; salt- forming counterions such as sodium; and/or nonionic surfactants such as TWEENTM, polyethylene glycol (PEG), and PLURONICSTM.
  • buffers such as phosphate, citrate, and other organic acids
  • antioxidants including ascorbic acid
  • a “liposome” is a small vesicle composed of various types of lipids, phospholipids and/or surfactant which is useful for delivery of a drug (such as a VEGF-C polypeptide or antibody thereto) to a mammal.
  • a drug such as a VEGF-C polypeptide or antibody thereto
  • the components of the liposome are commonly arranged in a bilayer formation, similar to the lipid arrangement of biological membranes.
  • anti-neoplastic composition refers to a composition useful in treating cancer comprising at least one active therapeutic agent, e.g., "anticancer agent.”
  • therapeutic agents include, but are limited to, e.g., chemotherapeutic agents, growth inhibitory agents, cytotoxic agents, agents used in radiation therapy, anti-angiogenesis agents, apoptotic agents, anti- tubulin agents, and other-agents to treat cancer, such as anti-HER-2 antibodies, anti- CD20 antibodies, an epidermal growth factor receptor (EGFR) antagonist (e.g., a tyrosine kinase inhibitor), HER1/EGFR inhibitor (e.g., erlotinib (Tarceva ), platelet derived growth factor inhibitors (e.g., GleevecTM (Imatinib Mesylate)), a COX-2 inhibitor (e.g., celecoxib), interferons, cytokines, antagonists (
  • EGFR epidermal growth factor receptor
  • cytotoxic agent refers to a substance that inhibits or prevents a cellular function and/or causes cell death or destruction.
  • the term is intended to include radioactive isotopes (e.g., At 211 , 1 131 , 1 125 , Y 90 , Re 186 , Re 188 ,
  • chemotherapeutic agents e.g., methotrexate, adriamicin, vinca alkaloids (vincristine, vinblastine, etoposide), doxorubicin, melphalan, mitomycin C, chlorambucil, daunorubicin or other intercalating agents, enzymes and fragments thereof such as nucleolytic enzymes, antibiotics, and toxins such as small molecule toxins or enzymatically active toxins of bacterial, fungal, plant or animal origin, including fragments and/or variants thereof, and the various antitumor or anticancer agents disclosed below.
  • Other cytotoxic agents are described below.
  • a tumoricidal agent causes destruction of tumor cells.
  • a "toxin” is any substance capable of having a detrimental effect on the growth or proliferation of a cell.
  • a "chemotherapeutic agent” is a chemical compound useful in the treatment of cancer.
  • chemotherapeutic agents include alkylating agents such as thiotepa and CYTOXAN® cyclosphosphamide; alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including
  • triethiylenethiophosphoramide and trimethylolomelamine triethiylenethiophosphoramide and trimethylolomelamine; acetogenins (especially bullatacin and bullatacinone); delta-9-tetrahydrocannabinol (dronabinol,
  • MARINOL® beta-lapachone
  • lapachol colchicines
  • betulinic acid a camptothecin (including the synthetic analogue topotecan (HYCAMTIN®), CPT-11 (irinotecan, CAMPTOSAR®), acetylcamptothecin, scopolectin, and 9-aminocamptothecin);
  • camptothecin including the synthetic analogue topotecan (HYCAMTIN®), CPT-11 (irinotecan, CAMPTOSAR®), acetylcamptothecin, scopolectin, and 9-aminocamptothecin
  • bryostatin callystatin; CC-1065 (including its adozelesin, carzelesin and bizelesin synthetic analogues); podophyllotoxin; podophyllinic acid; teniposide; cryptophycins (particularly cryptophycin 1 and cryptophycin 8); dolastatin; duocarmycin (including the synthetic analogues, KW-2189 and CB1-TM1); eleutherobin; pancratistatin; a sarcodictyin; spongistatin; nitrogen mustards such as chlorambucil, chlornaphazine, cholophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, uracil mustard; nitrosureas such as carmustine, chlorozotocin, fotem
  • calicheamicin especially calicheamicin gamma II and calicheamicin omegall (see, e.g., Agnew, Chem Intl. Ed. Engl., 33: 183-186 (1994)); dynemicin, including dynemicin A; an esperamicin; as well as neocarzinostatin chromophore and related chromoprotein enediyne antiobiotic chromophores), aclacinomysins, actinomycin, authramycin, azaserine, bleomycins, cactinomycin, carabicin, carminomycin, carzinophilin, chromomycinis, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin (including ADRIAMYCIN®, morpholino-doxorubicin, cyan
  • GEMZAR® anti-metabolites such as methotrexate, gemcitabine (GEMZAR®), tegafur (UFTORAL®), capecitabine (XELODA®), an epothilone, and 5-fluorouracil
  • folic acid analogues such as denopterin, methotrexate, pteropterin, trimetrexate
  • purine analogs such as fludarabine, 6-mercaptopurine, thiamiprine, thioguanine
  • pyrimidine analogs such as ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine;
  • androgens such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti- adrenals such as aminoglutethimide, mitotane, trilostane; folic acid replenisher such as frolinic acid; aceglatone; aldophosphamide glycoside;
  • defofamine demecolcine; diaziquone; elfornithine; elliptinium acetate; etoglucid; gallium nitrate; hydroxyurea; lentinan; lonidainine; maytansinoids such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidanmol; nitraerine; pentostatin; phenamet; pirarubicin; losoxantrone; 2-ethylhydrazide; procarbazine; PSK® polysaccharide complex (JHS Natural Products, Eugene, OR); razoxane; rhizoxin; sizofiran; spirogermanium; tenuazonic acid; triaziquone; 2,2',2"- trichlorotriethylamine; trichothecenes (especially T-2 toxin, verracurin A, roridin A and angu
  • TAXOL® paclitaxel
  • ABRAXANETM albumin-engineered nanoparticle formulation of paclitaxel
  • TXOTERE® docetaxel
  • chloranbucil 6-thioguanine; mercaptopurine; methotrexate; platinum analogs such as cisplatin and carboplatin; vinblastine (VELBAN®); platinum; etoposide (VP- 16); ifosfamide; mitoxantrone; vincristine (ONCOVIN®); oxaliplatin; leucovovin;
  • vinorelbine NVELBINE®
  • novantrone edatrexate
  • daunomycin aminopterin
  • ibandronate topoisomerase inhibitor RFS 2000
  • difluorometlhylornithine retinoids
  • retinoids such as retinoic acid
  • pharmaceutically acceptable salts, acids or derivatives of any of the above as well as combinations of two or more of the above such as CHOP, an abbreviation for a combined therapy of cyclophosphamide, doxorubicin, vincristine, and prednisolone
  • FOLFOX an abbreviation for a treatment regimen with oxaliplatin (ELOXATINTM) combined with 5-FU and leucovovin.
  • ELOXATINTM oxaliplatin
  • anti-hormonal agents that act to regulate, reduce, block, or inhibit the effects of hormones that can promote the growth of cancer, and are often in the form of systemic, or whole-body treatment. They may be hormones themselves. Examples include anti-estrogens and selective estrogen receptor modulators (SERMs), including, for example, tamoxifen (including
  • NOLVADEX® tamoxifen raloxifene
  • droloxifene 4- hydroxytamoxifen, trioxifene, keoxifene, LY117018, onapristone, and toremifene (FARESTON®); anti-progesterones; estrogen receptor down-regulators (ERDs); agents that function to suppress or shut down the ovaries, for example, leutinizing hormone -releasing hormone (LHRH) agonists such as leuprolide acetate (LUPRON® and ELIGARD®), goserelin acetate, buserelin acetate and tripterelin; other anti- androgens such as flutamide, nilutamide and bicalutamide; and aromatase inhibitors that inhibit the enzyme aromatase, which regulates estrogen production in the adrenal glands, such as, for example, 4(5)-imidazoles, aminoglutethimide, mege
  • LHRH
  • chemotherapeutic agents includes bisphosphonates such as clodronate (for example, BONEFOS® or OSTAC®), etidronate (DIDROCAL®), NE-58095, zoledronic acid/zoledronate (ZOMETA®), alendronate (FOSAMAX®), pamidronate (AREDIA®), tiludronate (SKELID®), or risedronate (ACTONEL®); as well as troxacitabine (a 1 ,3-dioxolane nucleoside cytosine analog); antisense oligonucleotides, particularly those that inhibit expression of genes in signaling pathways implicated in abherant cell proliferation, such as, for example, PKC-alpha, Raf, H-Ras, and epidermal growth factor receptor (EGF-R); vaccines such as THERATOPE® vaccine and gene therapy vaccines, for example, ALLOVECTIN® vaccine, LEUVECTIN® vaccine, and VAXID® vaccine;
  • COX-2 inhibitors such as celecoxib (CELEBREX®; 4-(5-(4- methylphenyl)-3 -(trifluoromethyl)- 1 H-pyrazol- 1 -yl) benzenesulfonamide; and pharmaceutically acceptable salts, acids or derivatives of any of the above.
  • a “growth inhibitory agent” when used herein refers to a compound or composition which inhibits growth of a cell (such as a cell expressing VEGF-C or cells acted upon by VEGF-C) either in vitro or in vivo.
  • growth inhibitory agent is growth inhibitory antibody that prevents or reduces proliferation of a cell expressing an antigen to which the antibody binds.
  • the growth inhibitory agent may be one which significantly reduces the percentage of cells in S phase. Examples of growth inhibitory agents include agents that block cell cycle progression (at a place other than S phase), such as agents that induce Gl arrest and M-phase arrest.
  • Classical M-phase blockers include the vincas (vincristine and vinblastine), taxanes, and topoisomerase II inhibitors such as doxorubicin, epirubicin, daunorubicin, etoposide, and bleomycin.
  • Those agents that arrest Gl also spill over into S-phase arrest, for example, DNA alkylating agents such as tamoxifen, prednisone, dacarbazine, mechlorethamine, cisplatin, methotrexate, 5-fluorouracil, and ara-C.
  • TAXOTERE® Rhone-Poulenc Rorer
  • TAXOL® Bristol-Myers Squibb
  • Paclitaxel and docetaxel promote the assembly of microtubules from tubulin dimers and stabilize microtubules by preventing depolymerization, which results in the inhibition of mitosis in cells.
  • the "pathology" of a disease includes all phenomena that compromise the well-being of the patient. For cancer, this includes, without limitation, abnormal or uncontrollable cell growth, metastasis, interference with the normal functioning of neighboring cells, release of cytokines or other secretory products at abnormal levels, suppression or aggravation of inflammatory or immunological response, etc.
  • prodrug refers to a precursor or derivative form of a pharmaceutically active substance that is less cytotoxic to tumor cells compared to the parent drug and is capable of being enzymatically activated or converted into the more active parent form.
  • Wilman "Prodrugs in Cancer Chemotherapy” Biochemical Society Transactions, 14, pp. 375- 382, 615th Meeting Harbor and Stella et al. (1985).
  • Prodrugs A Chemical Approach to Targeted Drug Delivery,” Directed Drug Delivery, Borchardt et al, (ed.), pp. 247- 267, Humana Press.
  • the prodrugs of this invention include, but are not limited to, phosphate-containing prodrugs, thiophosphate-containing prodrugs, sulfate-containing prodrugs, peptide-containing prodrugs, D-amino acid-modified prodrugs,
  • glycosylated prodrugs ⁇ -lactam-containing prodrugs, optionally substituted phenoxyacetamide-containing prodrugs or optionally substituted phenylacetamide- containing prodrugs, 5-f uorocytosine and other 5-fluorouridine prodrugs which can be converted into the more active cytotoxic free drug.
  • cytotoxic drugs that can be derivatized into a prodrug form for use in this invention include, but are not limited to, those chemotherapeutic agents described above.
  • a "small molecule” is defined herein to have a molecular weight below about 500 Daltons.
  • “Purified” means that a molecule is present in a sample at a concentration of at least 95% by weight, or at least 98% by weight of the sample in which it is contained.
  • An "isolated" nucleic acid molecule is a nucleic acid molecule that is separated from at least one other nucleic acid molecule with which it is ordinarily associated, for example, in its natural environment.
  • An isolated nucleic acid molecule further includes a nucleic acid molecule contained in cells that ordinarily express the nucleic acid molecule, but the nucleic acid molecule is present extrachromosomally or at a chromosomal location that is different from its natural chromosomal location.
  • vector is intended to refer to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked.
  • plasmid refers to a circular double stranded DNA into which additional DNA segments may be ligated.
  • phage vector refers to a viral vector, wherein additional DNA segments may be ligated into the viral genome.
  • viral vector capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors).
  • vectors e.g., non-episomal mammalian vectors
  • vectors can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome.
  • certain vectors are capable of directing the expression of genes to which they are operatively linked.
  • Such vectors are referred to herein as "recombinant expression vectors," or simply, "expression vectors.”
  • expression vectors of utility in recombinant DNA techniques are often in the form of plasmids.
  • plasmid and vector may be used interchangeably as the plasmid is the most commonly used form of vector.
  • Polynucleotide or “nucleic acid,” as used interchangeably herein, refer to polymers of nucleotides of any length, and include DNA and RNA.
  • the nucleotides can be deoxyribonucleotides, ribonucleotides, modified nucleotides or bases, and/or their analogs, or any substrate that can be incorporated into a polymer by DNA or RNA polymerase or by a synthetic reaction.
  • a polynucleotide may comprise modified nucleotides, such as methylated nucleotides and their analogs. If present, modification to the nucleotide structure may be imparted before or after assembly of the polymer.
  • sequence of nucleotides may be interrupted by non- nucleotide components.
  • a polynucleotide may comprise modification(s) made after synthesis, such as conjugation to a label.
  • modifications include, for example, "caps,” substitution of one or more of the naturally occurring nucleotides with an analog, internucleotide modifications such as, for example, those with uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoamidates, carbamates, etc.) and with charged linkages (e.g., phosphorothioates,
  • phosphorodithioates those containing pendant moieties, such as, for example, proteins (e.g., nucleases, toxins, antibodies, signal peptides, ply-L-lysine, etc.), those with intercalators (e.g., acridine, psoralen, etc.), those containing chelators (e.g., metals, radioactive metals, boron, oxidative metals, etc.), those containing alkylators, those with modified linkages (e.g., alpha anomeric nucleic acids, etc.), as well as unmodified forms of the polynucleotides(s).
  • proteins e.g., nucleases, toxins, antibodies, signal peptides, ply-L-lysine, etc.
  • intercalators e.g., acridine, psoralen, etc.
  • chelators e.g., metals, radioactive metals, boron, oxid
  • any of the hydroxyl groups ordinarily present in the sugars may be replaced, for example, by phosphonate groups, phosphate groups, protected by standard protecting groups, or activated to prepare additional linkages to additional nucleotides, or may be conjugated to solid or semisolid supports.
  • the 5' and 3' terminal OH can be phosphorylated or substituted with amines or organic capping group moieties of from 1 to 20 carbon atoms.
  • Other hydroxyls may also be derivatized to standard protecting groups.
  • Polynucleotides can also contain analogous forms of ribose or deoxyribose sugars that are generally known in the art, including, for example, 2'-0-methyl-, 2'-0-allyl-, 2'-fluoro- or 2'- azido-ribose, carbocyclic sugar analogs, a-anomeric sugars, epimeric sugars such as arabinose, xyloses or lyxoses, pyranose sugars, furanose sugars, sedoheptuloses, acyclic analogs, and basic nucleoside analogs such as methyl riboside.
  • One or more phosphodiester linkages may be replaced by alternative linking groups.
  • linking groups include, but are not limited to, embodiments wherein phosphate is replaced by P(0)S ("thioate”), P(S)S ("dithioate”), (0)NR 2 ("amidate”), P(0)R, P(0)OR', CO, or CH2 ("formacetal”), in which each R or R' is independently H or substituted or unsubstituted alkyl (1-20 C) optionally containing an ether (-0-) linkage, aryl, alkenyl, cycloalkyl, cycloalkenyl or araldyl. Not all linkages in a polynucleotide need be identical. The preceding description applies to all
  • RNA and DNA are examples of polynucleotides referred to herein, including RNA and DNA.
  • Olionucleotide generally refers to short, generally single-stranded, generally synthetic polynucleotides that are generally, but not necessarily, less than about 200 nucleotides in length.
  • oligonucleotide and “polynucleotide” are not mutually exclusive. The description above for polynucleotides is equally and fully applicable to oligonucleotides.
  • Percent (%) amino acid sequence identity with respect to a reference polypeptide sequence is defined as the percentage of amino acid residues in a candidate sequence that are identical with the amino acid residues in the reference polypeptide sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are within the skill in the art, for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN or Megalign (DNASTAR) software. Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithms needed to achieve maximal alignment over the full length of the sequences being compared. For purposes herein, however, % amino acid sequence identity values are generated using the sequence comparison computer program ALIGN-2. The ALIGN-2 sequence comparison computer program was authored by Genentech, Inc., and the source code has been filed with user
  • the ALIGN-2 program is publicly available from Genentech, Inc., South San Francisco, California, or may be compiled from the source code.
  • the ALIGN-2 program should be compiled for use on a UNIX operating system, preferably digital UNIX V4.0D. All sequence comparison parameters are set by the ALIGN-2 program and do not vary.
  • % amino acid sequence identity of a given amino acid sequence A to, with, or against a given amino acid sequence B is calculated as follows:
  • control sequences refers to DNA sequences necessary for the expression of an operably linked coding sequence in a particular host organism.
  • the control sequences that are suitable for prokaryotes include a promoter, optionally an operator sequence, and a ribosome binding site.
  • Eukaryotic cells are known to utilize promoters, polyadenylation signals, and enhancers.
  • Nucleic acid is "operably linked" when it is placed into a functional relationship with another nucleic acid sequence.
  • presequence or secretory leader is operably linked to DNA for a polypeptide if it is expressed as a preprotein that participates in the secretion of the polypeptide; a promoter or enhancer is operably linked to a coding sequence if it affects the transcription of the sequence; or a ribosome binding site is operably linked to a coding sequence if it is positioned so as to facilitate translation.
  • "operably linked" means that the DNA sequences being linked are contiguous, and, in the case of a secretory leader, contiguous and in reading phase. However, enhancers do not have to be contiguous. Linking is accomplished by ligation at convenient restriction sites. If such sites do not exist, the synthetic oligonucleotide adaptors or linkers are used in accordance with conventional practice.
  • the expressions "cell,” “cell line,” and “cell culture” are used interchangeably and all such designations include progeny.
  • the words “transformants” and “transformed cells” include the primary subject cell and cultures derived therefrom without regard for the number of transfers. It is also understood that all progeny may not be precisely identical in DNA content, due to deliberate or inadvertent mutations. Mutant progeny that have the same function or biological activity as screened for in the originally transformed cell are included. Where distinct designations are intended, it will be clear from the context.
  • cancer recurrence herein refers to a return of cancer following treatment.
  • cancer recurrence includes return of cancer in the breast, as well as distant recurrence, where the cancer returns outside of the breast.
  • compositions of the invention are provided.
  • VEGF-C is known to be a key modulator of angiogenesis, lymphangiogenesis and metastasis in many tumor models, and inhibition of the VEGF-C axis is considered a promising strategy for inhibiting the development of metastasis.
  • the studies underlying the present invention which are presented in the examples below, support an important role of VEGF-C in angiogenesis, tumor lymphangiogenesis and metastasis. Additionally, the data set forth in the Examples demonstrate the presence of functional lymphatic vessels within tumors and show that treating with anti-VEGF-C results in a reduction of these functional lymphatics. See also Figures 7-9, 12-14 and 22
  • the invention encompasses isolated antibody and polynucleotide embodiments.
  • an anti-VEGF-C antibody is purified.
  • compositions including pharmaceutical compositions, comprising an anti-VEGF-C antibody.
  • compositions comprise one or more antibodies that bind to VEGF-C, and/or one or more polynucleotides comprising sequences encoding one or more antibodies that bind to VEGF-C.
  • suitable carriers such as pharmaceutically acceptable excipients including buffers, which are well known in the art.
  • the anti-VEGF-C antibodies of the invention are monoclonal. In yet another embodiment, the anti-VEGF-C antibodies are polyclonal. Also encompassed within the scope of the invention are Fab, Fab', Fab'- SH and F(ab') 2 fragments of the anti-VEGF-C antibodies provided herein. These antibody fragments can be created by traditional means, such as enzymatic digestion, or may be generated by recombinant techniques. Such antibody fragments may be chimeric or humanized. These fragments are useful for the diagnostic and purposes set forth below. In one embodiment, an anti-VEGF-C antibody is a chimeric, humanized, or human antibody.
  • Monoclonal antibodies are obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. Thus, the modifier "monoclonal" indicates the character of the antibody as not being a mixture of discrete antibodies.
  • Exemplary monoclonal antibodies derived from a phage library are provided herein and described in Example 1. Those antibodies include, but not limited to VCl, VCl . l, VCl .2, VCl .3, VCl .4, VCl .5, VCl .6, VCl .7, VCl .8, VCl .9, VCl .10, VCl .11, VCl .12, VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VCl .12.9, VCl .12.10, VC3, VC4, VC4.2, VC4.3, VC4.4, VC4.5.
  • the sequences of the heavy and light chain variable domains of VCl, VCl .l, VC1.2, VC1.3, VC1.4, VC1.5, VC1.6, VC1.7, VCl .8, VC1.9, VCl .10, VCl .11, VCl .12, VCl .12.1, VCl .12.2, VCl .12.3, VCl .12.4, VCl .12.5, VCl .12.6, VCl .12.8, VCl .12.9, VCl .12.10, VC3, VC4, VC4.2, VC4.3, VC4.4, VC4.5 are shown in Figure la-f.
  • the sequences of the heavy and light chain variable domains of anti-VEGF-C antibodies are also shown in Figures 23-30 and 38-39.
  • VCl .12.10 have no amino acid residues at positions 100a, 100b, 100c, lOOd and lOOe.
  • the HVR-H3 sequence of antibody VC3 has no amino acid residue at position lOOd. See also Figures 24, 28 and 30.
  • the monoclonal antibodies that binds to VEGF-C or a fragment described herein further comprises an amino acid substitution at position 297 to alanine. In certain embodiments, the monoclonal antibodies that binds to VEGF-C or a fragment described herein further comprises an amino acid substitution at position 297 to alanine and at position 265 to alanine. In certain embodiments, anti-VEGF-C antibody VC4.5 comprises the amino acid substitution at position 297 with alanine. In certain embodiments, anti-VEGF-C antibody VC4.5 comprises the amino acid substitution at position 297 with alanine and at position 265 to alanine.
  • anti-VEGF-C antibodies VCl .12.1, VCl .12.4 and VC 1.12.9 comprise the amino acid substitution at position 297 with alanine.
  • anti-VEGF-C antibodies VCl .12.1, VCl .12.4 and VCl .12.9 comprise the amino acid substitution at position 297 with alanine and at position 265 to alanine.
  • antibodies comprising amino acid substitions at positions 265 and 297 with alanine are referred to as "DANA".
  • the invention provides an antibody comprising a HVR-Hl region comprising the sequence of SEQ ID NO: l, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4. In one aspect, the invention provides an antibody comprising a HVR-H2 region comprising the sequence of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO: 7 or SEQ ID NO:8.
  • the invention provides an antibody comprising a HVR-H3 region comprising the sequence of SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: l l, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25 or SEQ ID NO:26.
  • the invention provides an antibody comprising a HVR-Hl region comprising the sequence of SEQ ID NO: l, and a HVR-H2 region comprising the sequence of SEQ ID NO:6. In one embodiment, the invention provides an antibody comprising a HVR-Hl region comprising the sequence of SEQ ID NO: 1 , and a HVR-H3 region comprising the sequence of SEQ ID NO:21. In one embodiment, the invention provides an antibody comprising a HVR-H2 region comprising the sequence of SEQ ID NO:6, and a HVR-H3 region comprising the sequence of SEQ ID NO:21.
  • the invention provides an antibody comprising a HVR-Hl region comprising the sequence of SEQ ID NO: l, a HVR-H2 region comprising the sequence of SEQ ID NO:6, and a HVR-H3 region comprising the sequence of SEQ ID NO:21.
  • the invention provides an antibody comprising a HVR-Hl region comprising the sequence of SEQ ID NO:3, and a HVR-H2 region comprising the sequence of SEQ ID NO:8. In one embodiment, the invention provides an antibody comprising a HVR-Hl region comprising the sequence of SEQ ID NO:3, and a HVR-H3 region comprising the sequence of SEQ ID NO:26. In one embodiment, the invention provides an antibody comprising a HVR-H2 region comprising the sequence of SEQ ID NO: 8, and a HVR-H3 region comprising the sequence of SEQ ID NO:26.
  • the invention provides an antibody comprising a HVR-Hl region comprising the sequence of SEQ ID NO:3, a HVR-H2 region comprising the sequence of SEQ ID NO: 8, and a F1VR-H3 region comprising the sequence of SEQ ID NO:26.
  • the invention provides an antibody comprising at least one, at least two, or all three of the following:
  • the invention provides an antibody comprising at least one, at least two, or all three of the following:
  • the invention provides an antibody comprising at least one, at least two, or all three of the following:
  • the invention provides an antibody comprising at least one, at least two, or all three of the following:
  • the invention provides an antibody comprising at least one, at least two, or all three of the following:
  • the invention provides an antibody comprising at least one, at least two, or all three of the following:
  • the invention provides antibodies comprising heavy chain HVR sequences as depicted in Figure 1A-C. In one embodiment, the invention provides antibodies comprising heavy chain sequence SEQ ID NO: 73 or SEQ ID NO:84.
  • the invention provides antibodies comprising light chain HVR sequences as depicted in Figure 1D-F.
  • the invention provides antibodies comprising light chain sequence SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78, SEQ ID NO:79, SEQ ID NO:80. SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83 or SEQ ID NO:85.
  • any anti-VEGF-C antibody of the invention described herein amino acid asparagine at position 297 is substituted with amino acid alanine.
  • amino acid aspartic acid at position 265 is substituted with amino acid alanine.
  • the antibody further comprises heavy chain sequence SEQ ID NO: 73 or SEQ ID NO: 84.
  • the antibody further comprises light chain HVR sequences as depicted in Figure 1D-F.
  • the antibody further comprises light chain sequence SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78, SEQ ID NO:79, SEQ ID NO:80.
  • SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83 or SEQ ID NO:85 amino acid asparagine at position 297 is substituted with amino acid alanine.
  • amino acid aspartic acid at position 265 is substituted with amino acid alanine.
  • the antibody further comprises heavy chain sequence SEQ ID NO: 73 or
  • the antibodies of the invention comprise a light chain variable domain of humanized 4D5 antibody (huMAb4D5-8)
  • the huMAb4D5-8 light chain variable domain sequence is modified at one or more of positions 30, 66 and 91 (Asn, Arg and His as indicated in bold/italics above, respectively).
  • the modified huMAb4D5-8 sequence comprises Ser in position 30, Gly in position 66 and/or Ser in position 91.
  • an antibody of the invention comprises a light chain variable domain comprising the sequence depicted in SEQ ID NO: 72 below:
  • Antibodies of the invention can comprise any suitable framework variable domain sequence, provided binding activity to VEGF-C is substantially retained.
  • antibodies of the invention comprise a human subgroup III heavy chain framework consensus sequence.
  • the framework consensus sequence comprises substitution at position 71, 73 and/or 78.
  • position 71 is A
  • 73 is T
  • 78 is A.
  • these antibodies comprise heavy chain variable domain framework sequences of huMAb4D5-8 (HERCEPTIN ® , Genentech, Inc., South San Francisco, CA, USA) (also referred to in U.S. Pat. Nos.
  • these antibodies further comprise a human ⁇ light chain framework consensus sequence.
  • these antibodies comprise light chain HVR sequences of huMAb4D5-8 as described in U.S. Pat. Nos. 6,407,213 & 5,821,337).
  • these antibodies comprise light chain variable domain sequences of huMAb4D5-8 (HERCEPTIN ® , Genentech, Inc., South San Francisco, CA, USA) (also referred to in U.S. Pat. Nos. 6,407,213 & 5,821,337, and Lee et al, J. Mol. Biol. (2004), 340(5): 1073-93).
  • an antibody of the invention comprises a heavy chain variable domain, wherein the framework sequence comprises the sequences of SEQ ID NOs:40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, or 58 ( Figures 2A and 2B), and HVR HI, H2 and H3 sequences are SEQ ID NOS: l, 6 and/or 21, respectively.
  • an antibody of the invention comprises a light chain variable domain, wherein the framework sequence comprises the sequences of SEQ ID NOs:59, 60, 61 or 62 ( Figure 3), HVR LI and L2 sequences are SEQ ID NOS:27 and 28, respectively, and HVR L3 sequence is SEQ ID NOs:30, 31, 32, 33, 34, 35, 36, 37 or 38, respectively.
  • an antibody of the invention comprises a heavy chain variable domain, wherein the framework sequence comprises the sequences of SEQ ID NOs:40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, or 58, and HVR HI, H2 and H3 sequences are SEQ ID NOs:3, 8 and 26, respectively.
  • an antibody of the invention comprises a light chain variable domain, wherein the framework sequence comprises the sequences of SEQ ID NOs:59, 60, 61 andor 62, and HVR LI, L2 and L3 sequences are SEQ ID NOs:27, 28 and 29, respectively.
  • an antibody of the invention comprises a heavy chain variable domain comprising the sequence of SEQ ID NO: 73. In one embodiment, an antibody of the invention comprises a light chain variable domain comprising the sequence of SEQ ID NOs:74, 75, 76, 77, 78, 79, 80, 81, 82 or 83. In one embodiment, an antibody of the invention comprises a heavy chain variable domain comprising the sequence of SEQ ID NO: 84 and a light chain variable domain comprising the sequence of SEQ ID NO: 85.
  • the invention provides an antibody that competes with any of the above-mentioned antibodies for binding to VEGF-C. In another aspect, the invention provides an antibody that binds to the same epitope on VEGF-C as any of the above-mentioned antibodies.
  • the present invention encompasses antibody fragments.
  • Antibody fragments may be generated by traditional means, such as enzymatic digestion, or by recombinant techniques. In certain circumstances there are advantages of using antibody fragments, rather than whole antibodies. The smaller size of the fragments allows for rapid clearance, and may lead to improved access to solid tumors. For a review of certain antibody fragments, see Hudson et al. (2003) Nat. Med. 9: 129-134.
  • Antibody fragments can be isolated from the antibody phage libraries discussed above. Alternatively, Fab'-SH fragments can be directly recovered from E. coli and chemically coupled to form F(ab') 2 fragments (Carter et al., Bio/Technology 10: 163- 167 (1992)). According to another approach, F(ab') 2 fragments can be isolated directly from recombinant host cell culture. Fab and F(ab') 2 fragment with increased in vivo half-life comprising salvage receptor binding epitope residues are described in U.S. Pat. No. 5,869,046. Other techniques for the production of antibody fragments will be apparent to the skilled practitioner. In certain embodiments, an antibody is a single chain Fv fragment (scFv). See WO 93/16185; U.S. Pat. Nos. 5,571,894; and
  • Fv and scFv are the only species with intact combining sites that are devoid of constant regions; thus, they may be suitable for reduced nonspecific binding during in vivo use.
  • scFv fusion proteins may be constructed to yield fusion of an effector protein at either the amino or the carboxy terminus of an scFv. See Antibody Engineering, ed. Borrebaeck, supra.
  • the antibody fragment may also be a "linear antibody", e.g., as described in U.S. Pat. No. 5,641,870, for example. Such linear antibodies may be monospecific or bispecific.
  • the invention encompasses humanized antibodies.
  • Various methods for humanizing non-human antibodies are known in the art.
  • a humanized antibody can have one or more amino acid residues introduced into it from a source which is non-human. These non-human amino acid residues are often referred to as "import" residues, which are typically taken from an "import” variable domain.
  • Humanization can be essentially performed following the method of Winter and co-workers (Jones et al. (1986) Nature 321 :522-525; Riechmann et al. (1988) Nature 332:323-327; Verhoeyen et al. (1988) Science 239: 1534-1536), by
  • humanized antibodies are chimeric antibodies (U.S. Patent No. 4,816,567) wherein substantially less than an intact human variable domain has been substituted by the corresponding sequence from a non-human species.
  • humanized antibodies are typically human antibodies in which some hypervariable region residues and possibly some FR residues are substituted by residues from analogous sites in rodent antibodies.
  • variable domains both light and heavy
  • the choice of human variable domains, both light and heavy, to be used in making the humanized antibodies can be important to reduce antigenicity.
  • sequence of the variable domain of a rodent antibody is screened against the entire library of known human variable- domain sequences.
  • the human sequence which is closest to that of the rodent is then accepted as the human framework for the humanized antibody. See, e.g., Sims et al. (1993) J. Immunol. 151 :2296; Chothia et al. (1987) J. Mol. Biol. 196:901.
  • Another method uses a particular framework derived from the consensus sequence of all human antibodies of a particular subgroup of light or heavy chains.
  • the same framework may be used for several different humanized antibodies. See, e.g., Carter et al. (1992) Proc. Natl. Acad. Sci. USA, 89:4285; Presta et al. (1993) J. Immunol, 151 :2623.
  • humanized antibodies are prepared by a process of analysis of the parental sequences and various conceptual humanized products using three-dimensional models of the parental and humanized sequences.
  • Three-dimensional immunoglobulin models are commonly available and are familiar to those skilled in the art.
  • Computer programs are available which illustrate and display probable three-dimensional conformational structures of selected candidate immunoglobulin sequences. Inspection of these displays permits analysis of the likely role of the residues in the functioning of the candidate immunoglobulin sequence, i.e., the analysis of residues that influence the ability of the candidate immunoglobulin to bind its antigen.
  • FR residues can be selected and combined from the recipient and import sequences so that the desired antibody characteristic, such as increased affinity for the target antigen(s), is achieved.
  • the hypervariable region residues are directly and most substantially involved in influencing antigen binding.
  • Human antibodies of the invention can be constructed by combining Fv clone variable domain sequence(s) selected from human-derived phage display libraries with known human constant domain sequences(s) as described above.
  • human monoclonal antibodies of the invention can be made by the hybridoma method. Human myeloma and mouse-human heteromyeloma cell lines for the production of human monoclonal antibodies have been described, for example, by Kozbor J. Immunol, 133: 3001 (1984); Brodeur et al, Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (Marcel Dekker, Inc., New York, 1987); and Boerner et al, J. Immunol, 147: 86 (1991).
  • transgenic animals e.g. mice
  • transgenic animals e.g. mice
  • JH antibody heavy-chain joining region
  • transfer of the human germ-line immunoglobulin gene array in such germ-line mutant mice will result in the production of human antibodies upon antigen challenge.
  • Jakobovits et al Proc. Natl. Acad. Sci USA, 90: 2551 (1993); Jakobovits et al, Nature, 362: 255 (1993); Bruggermann et al, Year in Immunol, 7: 33 (1993).
  • Gene shuffling can also be used to derive human antibodies from non-human, e.g. rodent, antibodies, where the human antibody has similar affinities and specificities to the starting non-human antibody.
  • this method which is also called “epitope imprinting"
  • either the heavy or light chain variable region of a non-human antibody fragment obtained by phage display techniques as described herein is replaced with a repertoire of human V domain genes, creating a population of non-human chain/human chain scFv or Fab chimeras.
  • multispecific antibody of this invention includes an antibody that binds to VEGF-C and to another antigen.
  • multispecific antibodies may bind to two different epitopes of VEGF- C.
  • Multispecific antibodies may also be used to localize cytotoxic agents to cells which express VEGF-C. These antibodies possess a VEGF-C-binding arm and an arm which binds a cytotoxic agent, such as, e.g., saporin, anti-interferon-a, vinca alkaloid, ricin A chain, methotrexate or radioactive isotope hapten.
  • Multispecific antibodies can be prepared as full length antibodies or antibody fragments ⁇ e.g. F(ab') 2 bispecific antibodies).
  • antibody variable domains are fused to immunoglobulin constant domain sequences.
  • the fusion for example, is with an immunoglobulin heavy chain constant domain, comprising at least part of the hinge, CH2, and CH3 regions.
  • the first heavy-chain constant region (CHI) is present in at least one of the fusions.
  • immunoglobulin heavy chain fusions and, if desired, the immunoglobulin light chain are inserted into separate expression vectors, and are co-transfected into a suitable host organism.
  • This provides for great flexibility in adjusting the mutual proportions of the three polypeptide fragments in embodiments when unequal ratios of the three polypeptide chains used in the construction provide the optimum yields. It is, however, possible to insert the coding sequences for two or all three polypeptide chains in one expression vector when the expression of at least two polypeptide chains in equal ratios results in high yields or when the ratios are of no particular significance.
  • the bispecific antibodies are composed of a hybrid immunoglobulin heavy chain with a first binding specificity in one arm, and a hybrid immunoglobulin heavy chain-light chain pair (providing a second binding specificity) in the other arm. It was found that this asymmetric structure facilitates the separation of the desired bispecific compound from unwanted immunoglobulin chain combinations, as the presence of an immunoglobulin light chain in only one half of the bispecific molecule provides for a facile way of separation. This approach is disclosed in WO 94/04690. For further details of generating bispecific antibodies see, for example, Suresh et ah, Methods in
  • knock-into-hole or “KnH” technology refers to a technology that directs the pairing of two polypeptides together in vitro or in vivo by introducing a pertuberance (knob) into one polypeptide and a cavity (hole) into the other polypeptide at an interface in which they interact.
  • KnHs have been introduced in the Fc:Fc binding interfaces, CL:CH1 interfaces or VH/VL interfaces of antibodies (e.g., US20007/0178552, WO
  • multispecific antibodies having KnH in their Fc regions can further comprise single variable domains linked to each Fc region, or further comprise different heavy chain variable domains that pair with similar or different light chain variable domains.
  • one or more small amino acid side chains from the interface of the first antibody molecule are replaced with larger side chains ⁇ e.g. tyrosine or
  • Compensatory "cavities" of identical or similar size to the large side chain(s) are created on the interface of the second antibody molecule by replacing large amino acid side chains with smaller ones ⁇ e.g. alanine or threonine). This provides a mechanism for increasing the yield of the heterodimer over other unwanted end-products such as homodimers.
  • Multipecific antibodies include cross-linked or "heteroconjugate" antibodies.
  • one of the antibodies in the heteroconjugate can be coupled to avidin, the other to biotin.
  • Such antibodies have, for example, been proposed to target immune system cells to unwanted cells (US Patent No. 4,676,980), and for treatment of HIV infection (WO 91/00360, WO 92/00373, and EP
  • Heteroconjugate antibodies may be made using any convenient cross-linking method. Suitable cross-linking agents and techniques are known (e.g., US Patent No.
  • bispecific antibodies can be prepared using chemical linkage.
  • Brennan et al., Science, 229: 81 (1985) describe a procedure wherein intact antibodies are proteolytically cleaved to generate F(ab') 2 fragments. These fragments are reduced in the presence of the dithiol complexing agent sodium arsenite to stabilize vicinal dithiols and prevent
  • the Fab' fragments generated are then converted to thionitrobenzoate (TNB) derivatives.
  • TAB thionitrobenzoate
  • One of the Fab'-TNB derivatives is then reconverted to the Fab'-thiol by reduction with mercaptoethylamine and is mixed with an equimolar amount of the other Fab'-TNB derivative to form the bispecific antibody.
  • the bispecific antibodies produced can be used as agents for the selective immobilization of enzymes.
  • Fab'-SH fragments can be recovered from E. coli and can be chemically coupled to form bispecific antibodies. Shalaby et al, J. Exp. Med., 175: 217-225 (1992) describe the production of a fully humanized bispecific antibody F(ab') 2 molecule. Each Fab' fragment was separately secreted from E. coli and subjected to directed chemical coupling in vitro to form the bispecific antibody. The bispecific antibody thus formed was able to bind to cells overexpressing the HER2 receptor and normal human T cells, as well as trigger the lytic activity of human cytotoxic lymphocytes against human breast tumor targets.
  • bispecific antibodies have been produced using leucine zippers.
  • the leucine zipper peptides from the Fos and Jun proteins were linked to the Fab' portions of two different antibodies by gene fusion.
  • the antibody homodimers were reduced at the hinge region to form monomers and then re -oxidized to form the antibody heterodimers. This method can also be utilized for the production of antibody homodimers.
  • the fragments comprise a heavy-chain variable domain (VH) connected to a light-chain variable domain (VL) by a linker which is too short to allow pairing between the two domains on the same chain. Accordingly, the VH and VL domains of one fragment are forced to pair with the complementary VL and VH domains of another fragment, thereby forming two antigen-binding sites.
  • VH and VL domains of one fragment are forced to pair with the complementary VL and VH domains of another fragment, thereby forming two antigen-binding sites.
  • sFv single-chain Fv
  • Antibodies with more than two valencies are contemplated.
  • trispecific antibodies can be prepared. Tutt et al. J. Immunol. 147: 60 (1991).
  • an antibody of the invention is a single- domain antibody.
  • a single-domain antibody is a single polyeptide chain comprising all or a portion of the heavy chain variable domain or all or a portion of the light chain variable domain of an antibody.
  • a single-domain antibody is a human single-domain antibody (Domantis, Inc., Waltham, MA; see, e.g., U.S. Patent No. 6,248,516 Bl).
  • a single-domain antibody consists of all or a portion of the heavy chain variable domain of an antibody.
  • amino acid sequence modification(s) of the antibodies described herein are contemplated. For example, it may be desirable to improve the binding affinity and/or other biological properties of the antibody.
  • Amino acid sequence variants of the antibody may be prepared by introducing appropriate changes into the nucleotide sequence encoding the antibody, or by peptide synthesis. Such modifications include, for example, deletions from, and/or insertions into and/or substitutions of, residues within the amino acid sequences of the antibody. Any combination of deletion, insertion, and substitution can be made to arrive at the final construct, provided that the final construct possesses the desired characteristics.
  • the amino acid alterations may be introduced in the subject antibody amino acid sequence at the time that sequence is made.
  • a residue or group of target residues are identified ⁇ e.g., charged residues such as arg, asp, his, lys, and glu) and replaced by a neutral or negatively charged amino acid (e.g., alanine or poly alanine) to affect the interaction of the amino acids with antigen.
  • a neutral or negatively charged amino acid e.g., alanine or poly alanine
  • Those amino acid locations demonstrating functional sensitivity to the substitutions then are refined by introducing further or other variants at, or for, the sites of substitution.
  • the site for introducing an amino acid sequence variation is predetermined, the nature of the mutation per se need not be
  • ala scanning or random mutagenesis is conducted at the target codon or region and the expressed immunoglobulins are screened for the desired activity.
  • Amino acid sequence insertions include amino- and/or carboxyl- terminal fusions ranging in length from one residue to polypeptides containing a hundred or more residues, as well as intrasequence insertions of single or multiple amino acid residues.
  • terminal insertions include an antibody with an N- terminal methionyl residue.
  • Other insertional variants of the antibody molecule include the fusion to the N- or C-terminus of the antibody to an enzyme (e.g. for ADEPT) or a polypeptide which increases the serum half-life of the antibody.
  • an antibody of the invention is altered to increase or decrease the extent to which the antibody is glycosylated.
  • Glycosylation of polypeptides is typically either N-linked or O-linked.
  • N-linked refers to the attachment of a carbohydrate moiety to the side chain of an asparagine residue.
  • the tripeptide sequences asparagine-X-serine and asparagine-X-threonine, where X is any amino acid except proline, are the recognition sequences for enzymatic attachment of the carbohydrate moiety to the asparagine side chain.
  • the presence of either of these tripeptide sequences in a polypeptide creates a potential glycosylation site.
  • O- linked glycosylation refers to the attachment of one of the sugars N- aceylgalactosamine, galactose, or xylose to a hydroxyamino acid, most commonly serine or threonine, although 5-hydroxyproline or 5-hydroxylysine may also be used.
  • Addition or deletion of glycosylation sites to the antibody is conveniently accomplished by altering the amino acid sequence such that one or more of the above-described tripeptide sequences (for N-linked glycosylation sites) is created or removed.
  • the alteration may also be made by the addition, deletion, or substitution of one or more serine or threonine residues to the sequence of the original antibody (for O-linked glycosylation sites).
  • the carbohydrate attached thereto may be altered.
  • Native antibodies produced by mammalian cells typically comprise a branched, biantennary oligosaccharide that is generally attached by an N-linkage to Asn297 of the CH2 domain of the Fc region. See, e.g., Wright et al. (1997) TIBTECH 15:26-32.
  • the oligosaccharide may include various
  • oligosaccharide in an antibody of the invention may be made in order to create antibody variants with certain improved properties.
  • antibody variants are provided having a carbohydrate structure that lacks fucose attached (directly or indirectly) to an Fc region. Such variants may have improved ADCC function. See, e.g., US Patent Publication Nos. US 2003/0157108 (Presta, L.); US 2004/0093621 (Kyowa Hakko Kogyo Co., Ltd). Examples of publications related to "defucosylated” or "fucose-deficient" antibody variants include: US 2003/0157108; WO 2000/61739; WO 2001/29246; US
  • WO2002/031140 Okazaki et al. J. Mol. Biol. 336: 1239-1249 (2004); Yamane- Ohnuki et al. Biotech. Bioeng. 87: 614 (2004).
  • Examples of cell lines capable of producing defucosylated antibodies include Led 3 CHO cells deficient in protein fucosylation (Ripka et al. Arch. Biochem. Biophys.
  • knockout cell lines such as alpha- 1,6- fucosyltransferase gene, FUT8, knockout CHO cells (see, e.g., Yamane-Ohnuki et al. Biotech. Bioeng. 87: 614 (2004); Kanda, Y. et al, Biotechnol. Bioeng., 94(4):680-688 (2006); and WO2003/085107).
  • Antibodies variants are further provided with bisected
  • oligosaccharides e.g., in which a biantennary oligosaccharide attached to the Fc region of the antibody is bisected by GlcNAc.
  • Such antibody variants may have reduced fucosylation and/or improved ADCC function. Examples of such antibody variants are described, e.g., in WO 2003/011878 (Jean-Mairet et al); US Patent No. 6,602,684 (Umana et al); and US 2005/0123546 (Umana et al).
  • Antibody variants with at least one galactose residue in the oligosaccharide attached to the Fc region are also provided. Such antibody variants may have improved CDC function. Such antibody variants are described, e.g., in WO 1997/30087 (Patel et al); WO
  • an antibody variant comprises an Fc region with one or more amino acid substitutions which further improve ADCC, for example, substitutions at positions 298, 333, and/or 334 of the Fc region (Eu numbering of residues). Such substitutions may occur in combination with any of the variations described above.
  • an anti-VEGF-C antibody variant comprises an Fc region with an amino substitution at position 297.
  • the invention contemplates an antibody variant that possesses some but not all effector functions, which make it a desirable candidate for many applications in which the half life of the antibody in vivo is important yet certain effector functions (such as complement and ADCC) are unnecessary or deleterious.
  • the Fc activities of the antibody are measured to ensure that only the desired properties are maintained.
  • In vitro and/or in vivo cytotoxicity assays can be conducted to confirm the reduction/depletion of CDC and/or ADCC activities.
  • Fc receptor (FcR) binding assays can be conducted to ensure that the antibody lacks FcyR binding (hence likely lacking ADCC activity), but retains FcRn binding ability.
  • NK cells express FcyRIII only, whereas monocytes express FcyRI, FcyRII and FcyRIII.
  • FcR expression on hematopoietic cells is summarized in Table 3 on page 464 of Ravetch and Kinet, Annu. Rev. Immunol. 9:457-92 (1991).
  • Non-limiting examples of in vitro assays to assess ADCC activity of a molecule of interest is described in U.S. Patent No. 5,500,362 (see, e.g. Hellstrom, I., et al. Proc. Nat 7 Acad. Sci. USA 83:7059-7063 (1986)) and Hellstrom, I et al, Proc.
  • non-radioactive assays methods may be employed (see, for example, ACTITM non-radioactive cytotoxicity assay for flow cytometry (CellTechnology, Inc. Mountain View, CA; and CytoTox 96 non-radioactive cytotoxicity assay (Promega, Madison, WI).
  • Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and Natural Killer (NK) cells.
  • ADCC activity of the molecule of interest may be assessed in vivo, e.g., in a animal model such as that disclosed in Clynes et al. Proc. Nat 'lAcad. Sci. USA 95:652-656 (1998).
  • Clq binding assays may also be carried out to confirm that the antibody is unable to bind Clq and hence lacks CDC activity.
  • a CDC assay may be performed (see, for example, Gazzano-Santoro et al., J. Immunol. Methods 202: 163 (1996); Cragg, M.S. et al, Blood 101 : 1045-1052 (2003); and Cragg, M.S. and M.J.
  • FcRn binding and in vivo clearance/half life determinations can also be performed using methods known in the art (see, for example, Petkova, S.B. et al, Int 'l. Immunol. 18(12): 1759-1769 (2006)).
  • substitutions are provided. Sites of interest for substitutional mutagenesis include the hypervariable regions, but FR alterations are also contemplated. Conservative substitutions are shown in Table 1 under the heading of "preferred substitutions.” More substantial changes, denominated "exemplary substitutions" are provided in Table 1, or as further described below in reference to amino acid classes. Amino acid substitutions may be introduced into an antibody of interest and the products screened, e.g., for a desired activity, such as improved antigen binding, decreased immunogenicity, improved ADCC or CDC, etc.
  • Modifications in the biological properties of an antibody may be accomplished by selecting substitutions that affect (a) the structure of the polypeptide backbone in the area of the substitution, for example, as a sheet or helical
  • Amino acids may be grouped according to similarities in the properties of their side chains (in A. L. Lehninger, in Biochemistry, second ed., pp. 73-75, Worth Publishers, New York (1975)):
  • Naturally occurring residues may be divided into groups based on common side-chain properties:
  • Non-conservative substitutions will entail exchanging a member of one of these classes for another class. Such substituted residues also may be introduced into the conservative substitution sites or, into the remaining (non- conserved) sites.
  • substitutional variant involves substituting one or more hypervariable region residues of a parent antibody (e.g. a humanized or human antibody).
  • a parent antibody e.g. a humanized or human antibody
  • the resulting variant(s) selected for further development will have modified (e.g., improved) biological properties relative to the parent antibody from which they are generated.
  • An exemplary substitutional variant is an affinity matured antibody, which may be conveniently generated using phage display-based affinity maturation techniques. Briefly, several hypervariable region sites (e.g. 6-7 sites) are mutated to generate all possible amino acid substitutions at each site.
  • the antibodies thus generated are displayed from filamentous phage particles as fusions to at least part of a phage coat protein (e.g., the gene III product of Ml 3) packaged within each particle.
  • the phage-displayed variants are then screened for their biological activity (e.g. binding affinity).
  • scanning mutagenesis e.g., alanine scanning
  • contact residues and neighboring residues are candidates for substitution according to techniques known in the art, including those elaborated herein.
  • Nucleic acid molecules encoding amino acid sequence variants of the antibody are prepared by a variety of methods known in the art. These methods include, but are not limited to, isolation from a natural source (in the case of naturally occurring amino acid sequence variants) or preparation by oligonucleotide-mediated (or site-directed) mutagenesis, PCR mutagenesis, and cassette mutagenesis of an earlier prepared variant or a non-variant version of the antibody.
  • the Fc region variant may comprise a human Fc region sequence (e.g., a human IgGl, IgG2, IgG3 or IgG4 Fc region) comprising an amino acid modification (e.g. a substitution) at one or more amino acid positions including that of a hinge cysteine.
  • a human Fc region sequence e.g., a human IgGl, IgG2, IgG3 or IgG4 Fc region
  • an amino acid modification e.g. a substitution
  • an antibody of the invention may comprise one or more alterations as compared to the wild type counterpart antibody, e.g. in the Fc region. These antibodies would nonetheless retain substantially the same characteristics required for therapeutic utility as compared to their wild type counterpart. For example, it is thought that certain alterations can be made in the Fc region that would result in altered (i.e., either improved or diminished) Clq binding and/or Complement Dependent Cytotoxicity (CDC), e.g., as described in
  • the invention provides antibodies comprising modifications in the interface of Fc polypeptides comprising the Fc region, wherein the modifications facilitate and/or promote heterodimerization.
  • modifications comprise introduction of a protuberance into a first Fc polypeptide and a cavity into a second Fc polypeptide, wherein the protuberance is positionable in the cavity so as to promote complexing of the first and second Fc polypeptides.
  • Methods of generating antibodies with these modifications are known in the art, e.g., as described in U.S. Pat. No. 5,731,168.
  • cysteine engineered antibodies e.g., "thioMAbs”
  • one or more residues of an antibody are substituted with cysteine residues.
  • the substituted residues occur at accessible sites of the antibody.
  • reactive thiol groups are thereby positioned at accessible sites of the antibody and may be used to conjugate the antibody to other moieties, such as drug moieties or linker-drug moieties, as described further herein.
  • any one or more of the following residues may be substituted with cysteine: V205 (Kabat numbering) of the light chain; Al 18 (EU numbering) of the heavy chain; and S400 (EU numbering) of the heavy chain Fc region.
  • the antibodies of the present invention can be further modified to contain additional nonproteinaceous moieties that are known in the art and readily available.
  • the moieties suitable for derivatization of the antibody are water soluble polymers.
  • water soluble polymers include, but are not limited to, polyethylene glycol (PEG), copolymers of ethylene
  • glycol/propylene glycol carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinyl pyrrolidone, poly-1, 3-dioxolane, poly-l,3,6-trioxane, ethylene/maleic anhydride copolymer, polyaminoacids (either homopolymers or random copolymers), and dextran or poly(n- vinyl pyrrolidone)polyethylene glycol, propropylene glycol homopolymers, prolypropylene oxide/ethylene oxide co-polymers, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, and mixtures thereof.
  • dextran polyvinyl alcohol
  • polyvinyl pyrrolidone poly-1, 3-dioxolane
  • poly-l,3,6-trioxane ethylene/maleic anhydride copolymer
  • polyaminoacids either homopolymers or random copolymers
  • Polyethylene glycol propionaldehyde may have advantages in manufacturing due to its stability in water.
  • the polymer may be of any molecular weight, and may be branched or unbranched.
  • the number of polymers attached to the antibody may vary, and if more than one polymer are attached, they can be the same or different molecules. In general, the number and/or type of polymers used for derivatization can be determined based on considerations including, but not limited to, the particular properties or functions of the antibody to be improved, whether the antibody derivative will be used in a therapy under defined conditions, etc.
  • nonproteinaceous moiety that may be selectively heated by exposure to radiation
  • the nonproteinaceous moiety is a carbon nanotube (Kam et al, Proc. Natl. Acad. Sci. USA 102: 11600-11605 (2005)).
  • the radiation may be of any wavelength, and includes, but is not limited to, wavelengths that do not harm ordinary cells, but which heat the nonproteinaceous moiety to a temperature at which cells proximal to the antibody-nonproteinaceous moiety are killed.
  • the invention also provides immunoconjugates (interchangeably referred to as "antibody-drug conjugates,” or "ADCs") comprising an antibody conjugated to one or more cytotoxic agents, such as a chemotherapeutic agent, a drug, a growth inhibitory agent, a toxin (e.g., a protein toxin, an enzymatically active toxin of bacterial, fungal, plant, or animal origin, or fragments thereof), or a radioactive isotope (i.e., a radioconjugate).
  • cytotoxic agents such as a chemotherapeutic agent, a drug, a growth inhibitory agent, a toxin (e.g., a protein toxin, an enzymatically active toxin of bacterial, fungal, plant, or animal origin, or fragments thereof), or a radioactive isotope (i.e., a radioconjugate).
  • Immunoconjugates have been used for the local delivery of cytotoxic agents, i.e., drugs that kill or inhibit the growth or proliferation of cells, in the treatment of cancer (Lambert, J. (2005) Curr. Opinion in Pharmacology 5:543- 549; Wu et al (2005) Nature Biotechnology 23(9): 1137-1146; Payne, G. (2003) i 3:207-212; Syrigos and Epenetos (1999) Anticancer Research 19:605-614; Niculescu- Duvaz and Springer (1997) Adv. Drug Deliv. Rev. 26: 151-172; U.S. Pat. No.
  • Immunoconjugates allow for the targeted delivery of a drug moiety to a tumor, and intracellular accumulation therein, where systemic administration of unconjugated drugs may result in unacceptable levels of toxicity to normal cells as well as the tumor cells sought to be eliminated (Baldwin et al, Lancet (Mar. 15, 1986) pp. 603-05; Thorpe (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications (A. Pinchera et al, eds) pp. 475-506.
  • cytotoxic drugs may exert their cytotoxic effects by mechanisms including tubulin binding, DNA binding, or topoisomerase inhibition. Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands.
  • ZEVALIN® is an antibody- radioisotope conjugate composed of a murine IgGl kappa monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes and 11 lln or 90Y radioisotope bound by a thiourea linker-chelator (Wiseman et al (2000) Eur. Jour. Nucl. Med. 27(7):766-77; Wiseman et al (2002) 5W 99(12):4336-42; Witzig et al (2002) J. Clin. Oncol.
  • ZEVALIN has activity against B-cell non-Hodgkin's Lymphoma (NHL), administration results in severe and prolonged cytopenias in most patients.
  • MYLOTARGTM (gemtuzumab ozogamicin, Wyeth Pharmaceuticals), an antibody-drug conjugate composed of a huCD33 antibody linked to calicheamicin, was approved in 2000 for the treatment of acute myeloid leukemia by injection (Drugs of the Future (2000) 25(7):686; US Patent Nos.
  • Cantuzumab mertansine an antibody-drug conjugate composed of the huC242 antibody linked via the disulfide linker SPP to the maytansinoid drug moiety, DM1
  • CanAg such as colon, pancreatic, gastric, and other cancers.
  • MLN-2704 (Millennium Pharm., BZL Biologies, Immunogen Inc.), an antibody-drug conjugate composed of the anti-prostate specific membrane antigen (PSMA) monoclonal antibody linked to the maytansinoid drug moiety, DM1, is under development for the potential treatment of prostate tumors.
  • PSMA anti-prostate specific membrane antigen
  • AE auristatin E
  • MMAE monomethylauristatin
  • dolastatin were conjugated to chimeric monoclonal antibodies cBR96 (specific to Lewis Y on carcinomas) and cACIO (specific to CD30 on hematological malignancies) (Doronina et al (2003) Nature Biotechnol. 21(7):778-784) and are under therapeutic development.
  • an immunoconjugate comprises an antibody and a chemotherapeutic agent or other toxin.
  • Chemotherapeutic agents useful in the generation of immunoconjugates are described herein (e.g., above).
  • Enzymatically active toxins and fragments thereof that can be used include diphtheria A chain, nonbinding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa), ricin A chain, abrin A chain, modeccin A chain, alpha- sarcin, Aleurites fordii proteins, dianthin proteins, Phytolaca americana proteins (PAPI, PAP II, and PAP-S), momordica charantia inhibitor, curcin, crotin, sapaonaria officinalis inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin, and the tricothecenes. See, e.g.,
  • Conjugates of the antibody and cytotoxic agent are made using a variety of bifunctional protein-coupling agents such as N-succinimidyl-3-(2-pyridyldithiol) propionate (SPDP), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate HC1), active esters (such as disuccinimidyl suberate), aldehydes (such as glutaraldehyde), bis-azido compounds (such as bis (p-azidobenzoyl) hexanediamine), bis-diazonium derivatives (such as bis-(p-diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as toluene 2,6-diisocyanate), and bis-active fluorine compounds (such as l,5-difluoro
  • SPDP N-succinimidyl-3-(2-pyridyl
  • a ricin immunotoxin can be prepared as described in Vitetta et al., Science, 238: 1098 (1987).
  • Carbon- 14-labeled 1-isothiocyanatobenzyl- 3-methyldiethylene triaminepentaacetic acid (MX-DTPA) is an exemplary chelating agent for conjugation of radionucleotide to the antibody. See W094/11026.
  • Conjugates of an antibody and one or more small molecule toxins such as a calicheamicin, maytansinoids, dolastatins, aurostatins, a trichothecene, and CC1065, and the derivatives of these toxins that have toxin activity, are also contemplated herein. Maytansine and maytansinoids
  • the immunoconjugate comprises an antibody (full length or fragments) conjugated to one or more maytansinoid molecules.
  • Maytansinoids are mitototic inhibitors which act by inhibiting tubulin polymerization. Maytansine was first isolated from the east African shrub Maytenus serrata (U.S. Patent No. 3,896,111). Subsequently, it was discovered that certain microbes also produce maytansinoids, such as maytansinol and C-3 maytansinol esters (U.S. Patent No. 4,151,042). Synthetic maytansinol and derivatives and analogues thereof are disclosed, for example, in U.S. Patent Nos. 4,137,230; 4,248,870; 4,256,746; 4,260,608; 4,265,814; 4,294,757; 4,307,016;
  • Maytansinoid drug moieties are attractive drug moieties in antibody drug conjugates because they are: (i) relatively accessible to prepare by fermentation or chemical modification, derivatization of fermentation products, (ii) amenable to derivatization with functional groups suitable for conjugation through the non-disulfide linkers to antibodies, (iii) stable in plasma, and (iv) effective against a variety of tumor cell lines.
  • Immunoconjugates containing maytansinoids, methods of making same, and their therapeutic use are disclosed, for example, in U.S. Patent Nos.
  • Antibody-maytansinoid conjugates are prepared by chemically linking an antibody to a maytansinoid molecule without significantly diminishing the biological activity of either the antibody or the maytansinoid molecule. See, e.g., U.S. Patent No. 5,208,020 (the disclosure of which is hereby expressly incorporated by reference). An average of 3-4 maytansinoid molecules conjugated per antibody molecule has shown efficacy in enhancing cytotoxicity of target cells without negatively affecting the function or solubility of the antibody, although even one molecule of toxin/antibody would be expected to enhance cytotoxicity over the use of naked antibody. Maytansinoids are well known in the art and can be synthesized by known techniques or isolated from natural sources.
  • Suitable maytansinoids are disclosed, for example, in U.S. Patent No. 5,208,020 and in the other patents and nonpatent publications referred to hereinabove.
  • Preferred maytansinoids are maytansinol and maytansinol analogues modified in the aromatic ring or at other positions of the maytansinol molecule, such as various maytansinol esters.
  • Antibody-maytansinoid conjugates comprising the linker component SMCC may be prepared as disclosed in U.S. Patent Application No. 10/960,602, filed Oct. 8, 2004.
  • the linking groups include disulfide groups, thioether groups, acid labile groups, photolabile groups, peptidase labile groups, or esterase labile groups, as disclosed in the above- identified patents, disulfide and thioether groups being preferred. Additional linking groups are described and exemplified herein.
  • Conjugates of the antibody and maytansinoid may be made using a variety of bifunctional protein coupling agents such as N-succinimidyl-3-(2- pyridyldithio) propionate (SPDP), succinimidyl-4-(N-maleimidomethyl) cyclohexane- 1-carboxylate (SMCC), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate HC1), active esters (such as disuccinimidyl suberate), aldehydes (such as glutaraldehyde), bis-azido compounds (such as bis (p- azidobenzoyl) hexanediamine), bis-diazonium derivatives (such as bis-(p- diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as toluene 2,6- diisocyanate), and bis-active fluorine compounds
  • Particularly preferred coupling agents include N-succinimidyl-3-(2- pyridyldithio) propionate (SPDP) (Carlsson et al, Biochem. J. 173:723-737 (1978)) and N-succinimidyl-4-(2-pyridylthio)pentanoate (SPP) to provide for a disulfide linkage.
  • SPDP N-succinimidyl-3-(2- pyridyldithio) propionate
  • SPP N-succinimidyl-4-(2-pyridylthio)pentanoate
  • the linker may be attached to the maytansinoid molecule at various positions, depending on the type of the link.
  • an ester linkage may be formed by reaction with a hydroxyl group using conventional coupling techniques. The reaction may occur at the C-3 position having a hydroxyl group, the C-14 position modified with hydroxymethyl, the C-15 position modified with a hydroxyl group, and the C-20 position having a hydroxyl group.
  • the linkage is formed at the C-3 position of maytansinol or a maytansinol analogue.
  • the immunoconjugate comprises an antibody conjugated to dolastatins or dolostatin peptidic analogs and derivatives, the auristatins (US Patent Nos. 5635483; 5780588). Dolastatins and auristatins have been shown to interfere with microtubule dynamics, GTP hydrolysis, and nuclear and cellular division (Woyke et al (2001) Antimicrob. Agents and Chemother.
  • the dolastatin or auristatin drug moiety may be attached to the antibody through the N (amino) terminus or the C (carboxyl) terminus of the peptidic drug moiety (WO 02/088172).
  • Exemplary auristatin embodiments include the N-terminus linked monomethylauristatin drug moieties DE and DF, disclosed in "Monomethylvaline Compounds Capable of Conjugation to Ligands", US Ser. No. 10/983,340, filed Nov. 5, 2004, the disclosure of which is expressly incorporated by reference in its entirety.
  • peptide-based drug moieties can be prepared by forming a peptide bond between two or more amino acids and/or peptide fragments.
  • Such peptide bonds can be prepared, for example, according to the liquid phase synthesis method (see E. Schroder and K. Liibke, "The Peptides", volume 1, pp 76-136, 1965, Academic Press) that is well known in the field of peptide chemistry.
  • the auristatin/dolastatin drug moieties may be prepared according to the methods of: US 5635483; US 5780588; Pettit et al (1989) J. Am. Chem. Soc.
  • the immunoconjugate comprises an antibody conjugated to one or more calicheamicin molecules.
  • the calicheamicin family of antibiotics are capable of producing double-stranded DNA breaks at sub- picomolar concentrations.
  • For the preparation of conjugates of the calicheamicin family see U.S. patents 5,712,374, 5,714,586, 5,739,116, 5,767,285, 5,770,701, 5,770,710, 5,773,001, 5,877,296 (all to American Cyanamid Company).
  • Structural analogues of calicheamicin which may be used include, but are not limited to, ⁇ , ⁇ x2I, a3I, N-acetyl- ⁇ , PSAG and ⁇ 1 (Hinman et al, Cancer Research 53:3336-3342 (1993), Lode et al, Cancer Research 58:2925-2928 (1998) and the aforementioned U.S. patents to American Cyanamid).
  • Another anti-tumor drug that the antibody can be conjugated is QFA which is an antifolate.
  • QFA is an antifolate.
  • Both calicheamicin and QFA have intracellular sites of action and do not readily cross the plasma membrane. Therefore, cellular uptake of these agents through antibody mediated internalization greatly enhances their cytotoxic effects.
  • antitumor agents that can be conjugated to the antibodies include BCNU, streptozoicin, vincristine and 5-fluorouracil, the family of agents known collectively LL-E33288 complex described in U.S. patents 5,053,394, 5,770,710, as well as esperamicins (U.S. patent 5,877,296).
  • Enzymatically active toxins and fragments thereof which can be used include diphtheria A chain, nonbinding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa), ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin, Aleurites fordii proteins, dianthin proteins, Phytolaca americana proteins (PAPI, PAPII, and PAP-S), momordica charantia inhibitor, curcin, crotin, sapaonaria officinalis inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin and the tricothecenes. See, for example, WO 93/21232 published October 28, 1993.
  • the present invention further contemplates an immunoconjugate formed between an antibody and a compound with nucleolytic activity (e.g., a ribonuclease or a DNA endonuclease such as a deoxyribonuclease; DNase).
  • a compound with nucleolytic activity e.g., a ribonuclease or a DNA endonuclease such as a deoxyribonuclease; DNase.
  • the antibody may comprise a highly radioactive atom.
  • a variety of radioactive isotopes are available for the production of radioconjugated antibodies. Examples include At 211 , 1 131 , 1 125 , Y 90 , Re 186 , Re 188 , Sm 153 , Bi 212 , P 32 , Pb 212 and radioactive isotopes of Lu.
  • the conjugate When used for detection, it may comprise a radioactive atom for scintigraphic studies, for example tc99m or 1123, or a spin label for nuclear magnetic resonance (NMR) imaging (also known as magnetic resonance imaging, mri), such as iodine- 123 again, iodine-131, indium-I l l, fluorine- 19, carbon- 13, nitrogen-15, oxygen- 17, gadolinium, manganese or iron.
  • NMR nuclear magnetic resonance
  • the radio- or other labels may be incorporated in the conjugate in known ways.
  • the peptide may be biosynthesized or may be synthesized by chemical amino acid synthesis using suitable amino acid precursors involving, for example, fluorine- 19 in place of hydrogen.
  • Labels such as tc 99 m or l 123 , Re 186 , Re 188 and In 111 can be attached via a cysteine residue in the peptide.
  • Yttrium-90 can be attached via a lysine residue.
  • the IODOGEN method (Fraker et al (1978) Biochem. Biophys. Res. Commun. 80: 49-57) can be used to incorporate iodine-123.
  • Conjugates of the antibody and cytotoxic agent may be made using a variety of bifunctional protein coupling agents such as N-succinimidyl-3-(2- pyridyldithio) propionate (SPDP), succinimidyl-4-(N-maleimidomethyl) cyclohexane- 1-carboxylate (SMCC), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate HC1), active esters (such as disuccinimidyl suberate), aldehydes (such as glutaraldehyde), bis-azido compounds (such as bis (p- azidobenzoyl) hexanediamine), bis-diazonium derivatives (such as bis-(p- diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as toluene 2,6- diisocyanate), and bis-active fluorine compounds (such as N-
  • a ricin immunotoxin can be prepared as described in Vitetta et al, Science 238: 1098 (1987).
  • Carbon- 14-labeled l-isothiocyanatobenzyl-3- methyldiethylene triaminepentaacetic acid (MX-DTPA) is an exemplary chelating agent for conjugation of radionucleotide to the antibody. See W094/11026.
  • the linker may be a "cleavable linker" facilitating release of the cytotoxic drug in the cell.
  • an acid-labile linker for example, an acid-labile linker, peptidase-sensitive linker, photolabile linker, dimethyl linker or disulfide-containing linker (Chari et al., Cancer Research 52: 127- 131 (1992); U.S. Patent No. 5,208,020) may be used.
  • the compounds expressly contemplate, but are not limited to, ADC prepared with cross-linker reagents: BMPS, EMCS, GMBS, HBVS, LC-SMCC, MBS, MPBH, SBAP, SIA, SIAB, SMCC, SMPB, SMPH, sulfo-EMCS, sulfo-GMBS, sulfo-KMUS, sulfo-MBS, sulfo-SIAB, sulfo-SMCC, and sulfo-SMPB, and SVSB (succinimidyl-(4-vinylsulfone)benzoate) which are commercially available (e.g., from Pierce Biotechnology, Inc., Rockford, IL., U.S.A). See pages 467-498, 2003-2004 Applications Handbook and Catalog.
  • an antibody is conjugated to one or more drug moieties (D), e.g. about 1 to about 20 drug moieties per antibody, through a linker (L).
  • the ADC of Formula I may be prepared by several routes, employing organic chemistry reactions, conditions, and reagents known to those skilled in the art, including: (1) reaction of a nucleophilic group of an antibody with a bivalent linker reagent, to form Ab-L, via a covalent bond, followed by reaction with a drug moiety D; and (2) reaction of a nucleophilic group of a drug moiety with a bivalent linker reagent, to form D-L, via a covalent bond, followed by reaction with the nucleophilic group of an antibody. Additional methods for preparing ADC are described herein.
  • the linker may be composed of one or more linker components.
  • exemplary linker components include 6-maleimidocaproyl ("MC"),
  • the linker may comprise amino acid residues.
  • exemplary amino acid linker components include a dipeptide, a tripeptide, a tetrapeptide or a pentapeptide.
  • Exemplary dipeptides include: valine-citrulline (vc or val-cit), alanine -phenylalanine (af or ala-phe).
  • Exemplary tripeptides include:
  • glycine-valine-citrulline gly-val-cit
  • glycine-glycine-glycine gly-glycine-glycine
  • Amino acid residues which comprise an amino acid linker component include those occurring naturally, as well as minor amino acids and non-naturally occurring amino acid analogs, such as citrulline.
  • Amino acid linker components can be designed and optimized in their selectivity for enzymatic cleavage by a particular enzymes, for example, a tumor-associated protease, cathepsin B, C and D, or a plasmin protease.
  • Nucleophilic groups on antibodies include, but are not limited to: (i) N-terminal amine groups, (ii) side chain amine groups, e.g. lysine, (iii) side chain thiol groups, e.g. cysteine, and (iv) sugar hydroxyl or amino groups where the antibody is glycosylated.
  • Amine, thiol, and hydroxyl groups are nucleophilic and capable of reacting to form covalent bonds with electrophilic groups on linker moieties and linker reagents including: (i) active esters such as NHS esters, HOBt esters, haloformates, and acid halides; (ii) alkyl and benzyl halides such as
  • haloacetamides (iii) aldehydes, ketones, carboxyl, and maleimide groups.
  • Certain antibodies have reducible interchain disulfides, i.e. cysteine bridges.
  • Antibodies may be made reactive for conjugation with linker reagents by treatment with a reducing agent such as DTT (dithiothreitol). Each cysteine bridge will thus form, theoretically, two reactive thiol nucleophiles.
  • Additional nucleophilic groups can be introduced into antibodies through the reaction of lysines with 2-iminothiolane (Traut's reagent) resulting in conversion of an amine into a thiol.
  • Reactive thiol groups may be introduced into the antibody (or fragment thereof) by introducing one, two, three, four, or more cysteine residues (e.g., preparing mutant antibodies comprising one or more non-native cysteine amino acid residues).
  • Antibody drug conjugates may also be produced by modification of the antibody to introduce electrophilic moieties, which can react with nucleophilic substituents on the linker reagent or drug.
  • the sugars of glycosylated antibodies may be oxidized, e.g. with periodate oxidizing reagents, to form aldehyde or ketone groups which may react with the amine group of linker reagents or drug moieties.
  • the resulting imine Schiff base groups may form a stable linkage, or may be reduced, e.g. by borohydride reagents to form stable amine linkages.
  • reaction of the carbohydrate portion of a glycosylated antibody with either glactose oxidase or sodium meta-periodate may yield carbonyl (aldehyde and ketone) groups in the protein that can react with appropriate groups on the drug (Hermanson, Bioconjugate Techniques).
  • proteins containing N-terminal serine or threonine residues can react with sodium meta-periodate, resulting in production of an aldehyde in place of the first amino acid (Geoghegan & Stroh, (1992) Bioconjugate Chem. 3: 138-146; US 5362852).
  • Such aldehyde can be reacted with a drug moiety or linker nucleophile.
  • nucleophilic groups on a drug moiety include, but are not limited to: amine, thiol, hydroxyl, hydrazide, oxime, hydrazine, thiosemicarbazone, hydrazine carboxylate, and arylhydrazide groups capable of reacting to form covalent bonds with electrophilic groups on linker moieties and linker reagents including: (i) active esters such as NHS esters, HOBt esters, haloformates, and acid halides; (ii) alkyl and benzyl halides such as haloacetamides; (iii) aldehydes, ketones, carboxyl, and maleimide groups.
  • a fusion protein comprising the antibody and cytotoxic agent may be made, e.g., by recombinant techniques or peptide synthesis.
  • the length of DNA may comprise respective regions encoding the two portions of the conjugate either adjacent one another or separated by a region encoding a linker peptide which does not destroy the desired properties of the conjugate.
  • the antibody may be conjugated to a "receptor" (such streptavidin) for utilization in tumor pre-targeting wherein the antibody-receptor conjugate is administered to the patient, followed by removal of unbound conjugate from the circulation using a clearing agent and then administration of a "ligand” (e.g., avidin) which is conjugated to a cytotoxic agent (e.g., a
  • Monoclonal antibodies of the invention can be made using the hybridoma method first described by Kohler et al., Nature, 256:495 (1975), and further described, e.g., in Hongo et al., Hybridoma, 14 (3): 253-260 (1995), Harlow et al., Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988); Hammerling et al., in: Monoclonal Antibodies and T-Cell Hybridomas 563- 681 (Elsevier, N.Y., 1981), and Ni, Xiandai Mianyixue, 26(4):265-268 (2006) regarding human-human hybridomas.
  • An exemplary protocol for producing monoclonal antibodies using the hybridoma method is described as follows.
  • a mouse or other appropriate host animal such as a hamster, is immunized to elicit lymphocytes that produce or are capable of producing antibodies that will specifically bind to the protein used for immunization.
  • Antibodies are raised in animals by multiple subcutaneous (sc) or intraperitoneal (ip) injections of a polypeptide comprising VEGF-C or a fragment thereof, and an adjuvant, such as monophosphoryl lipid A (MPL)/trehalose dicrynomycolate (TDM) (Ribi Immunochem. Research, Inc., Hamilton, MT).
  • MPL monophosphoryl lipid A
  • TDM trehalose dicrynomycolate
  • Serum from immunized animals is assayed for anti- VEGF-C antibodies, and booster immunizations are optionally administered. Lymphocytes from animals producing anti-VEGF-C antibodies are isolated.
  • lymphocytes may be immunized in vitro.
  • Lymphocytes are then fused with myeloma cells using a suitable fusing agent, such as polyethylene glycol, to form a hybridoma cell.
  • a suitable fusing agent such as polyethylene glycol
  • Myeloma cells may be used that fuse efficiently, support stable high- level production of antibody by the selected antibody-producing cells, and are sensitive to a medium such as HAT medium.
  • Exemplary myeloma cells include, but are not limited to, murine myeloma lines, such as those derived from MOPC-21 and MPC-11 mouse tumors available from the Salk Institute Cell Distribution Center, San Diego, California USA, and SP-2 or X63-Ag8-653 cells available from the American Type Culture Collection, Rockville, Maryland USA.
  • Human myeloma and mouse- human heteromyeloma cell lines also have been described for the production of human monoclonal antibodies (Kozbor, J. Immunol, 133:3001 (1984); Brodeur et al, Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (Marcel Dekker, Inc., New York, 1987)).
  • the hybridoma cells thus prepared are seeded and grown in a suitable culture medium, e.g., a medium that contains one or more substances that inhibit the growth or survival of the unfused, parental myeloma cells.
  • a suitable culture medium e.g., a medium that contains one or more substances that inhibit the growth or survival of the unfused, parental myeloma cells.
  • the culture medium for the hybridomas typically will include hypoxanthine, aminopterin, and thymidine (HAT medium), which substances prevent the growth of HGPRT-deficient cells.
  • serum-free hybridoma cell culture methods are used to reduce use of animal-derived serum such as fetal bovine serum, as described, for example, in Even et al, Trends in Biotechnology, 24(3), 105- 108 (2006).
  • Oligopeptides as tools for improving productivity of hybridoma cell cultures are described in Franek, Trends in Monoclonal Antibody Research, 111- 122 (2005). Specifically, standard culture media are enriched with certain amino acids (alanine, serine, asparagine, proline), or with protein hydrolyzate fractions, and apoptosis may be significantly suppressed by synthetic oligopeptides, constituted of three to six amino acid residues. The peptides are present at millimolar or higher concentrations.
  • Culture medium in which hybridoma cells are growing may be assayed for production of monoclonal antibodies that bind to VEGF-C.
  • the binding specificity of monoclonal antibodies produced by hybridoma cells may be determined by immunoprecipitation or by an in vitro binding assay, such as radioimmunoassay (RIA) or enzyme-linked immunoadsorbent assay (ELISA).
  • RIA radioimmunoassay
  • ELISA enzyme-linked immunoadsorbent assay
  • the binding affinity of the monoclonal antibody can be determined, for example, by Scatchard analysis. See, e.g., Munson et al, Anal. Biochem., 107:220 (1980).
  • the clones may be subcloned by limiting dilution procedures and grown by standard methods. See, e.g., Goding, supra.
  • Suitable culture media for this purpose include, for example, D-MEM or RPMI-1640 medium.
  • hybridoma cells may be grown in vivo as ascites tumors in an animal. Monoclonal antibodies secreted by the subclones are suitably separated from the culture medium, ascites fluid, or serum by conventional immunoglobulin purification procedures such as, for example, protein A-Sepharose, hydroxylapatite chromatography, gel electrophoresis, dialysis, or affinity chromatography.
  • immunoglobulin purification procedures such as, for example, protein A-Sepharose, hydroxylapatite chromatography, gel electrophoresis, dialysis, or affinity chromatography.
  • the method includes using minimal salts, such as lyotropic salts, in the binding process and preferably also using small amounts of organic solvents in the elution process.
  • Antibodies of the invention can be made by using combinatorial libraries to screen for antibodies with the desired activity or activities.
  • a variety of methods are known in the art for generating phage display libraries and screening such libraries for antibodies possessing the desired binding characteristics. Such methods are described generally in Hoogenboom et al. in Methods in Molecular Biology 178: 1-37 (O'Brien et al, ed., Human Press, Totowa, NJ, 2001).
  • one method of generating antibodies of interest is through the use of a phage antibody library as described in Lee et al, J. Mol. Biol. (2004), 340(5): 1073-93.
  • synthetic antibody clones are selected by screening phage libraries containing phage that display various fragments of antibody variable region (Fv) fused to phage coat protein. Such phage libraries are panned by affinity chromatography against the desired antigen. Clones expressing Fv fragments capable of binding to the desired antigen are adsorbed to the antigen and thus separated from the non-binding clones in the library. The binding clones are then eluted from the antigen, and can be further enriched by additional cycles of antigen
  • Fv antibody variable region
  • any of the antibodies of the invention can be obtained by designing a suitable antigen screening procedure to select for the phage clone of interest followed by construction of a full length antibody clone using the Fv sequences from the phage clone of interest and suitable constant region (Fc) sequences described in Kabat et al. , Sequences of Proteins of Immunological Interest, Fifth Edition, NIH Publication 91-3242, Bethesda MD (1991), vols. 1-3.
  • the antigen-binding domain of an antibody is formed from two variable (V) regions of about 110 amino acids, one each from the light (VL) and heavy (VH) chains, that both present three hypervariable loops (HVRs) or complementarity-determining regions (CDRs).
  • V variable
  • VH variable
  • CDRs complementarity-determining regions
  • Variable domains can be displayed functionally on phage, either as single-chain Fv (scFv) fragments, in which VH and VL are covalently linked through a short, flexible peptide, or as Fab fragments, in which they are each fused to a constant domain and interact non-covalently, as described in Winter et ⁇ ., ⁇ . Rev. Immunol, 12: 433-455 (1994).
  • scFv encoding phage clones and Fab encoding phage clones are collectively referred to as "Fv phage clones" or "Fv clones.”
  • Repertoires of VH and VL genes can be separately cloned by polymerase chain reaction (PCR) and recombined randomly in phage libraries, which can then be searched for antigen-binding clones as described in Winter et al., Ann. Rev. Immunol., 12: 433-455 (1994).
  • Libraries from immunized sources provide high- affinity antibodies to the immunogen without the requirement of constructing hybridomas.
  • the naive repertoire can be cloned to provide a single source of human antibodies to a wide range of non-self and also self antigens without any immunization as described by Griffiths et ⁇ ., ⁇ J 12: 725-734 (1993).
  • naive libraries can also be made synthetically by cloning the unrearranged V- gene segments from stem cells, and using PCR primers containing random sequence to encode the highly variable CDR3 regions and to accomplish rearrangement in vitro as described by Hoogenboom and Winter, J. Mol. Biol, 227: 381-388 (1992).
  • filamentous phage is used to display antibody fragments by fusion to the minor coat protein pill.
  • the antibody fragments can be displayed as single chain Fv fragments, in which VH and VL domains are connected on the same polypeptide chain by a flexible polypeptide spacer, e.g. as described by Marks et al., J. Mol. Biol, 222: 581-597 (1991), or as Fab fragments, in which one chain is fused to pill and the other is secreted into the bacterial host cell periplasm where assembly of a Fab-coat protein structure which becomes displayed on the phage surface by displacing some of the wild type coat proteins, e.g.
  • nucleic acids encoding antibody gene fragments are obtained from immune cells harvested from humans or animals. If a library biased in favor of anti-VEGF-C clones is desired, the subject is immunized with VEGF-C to generate an antibody response, and spleen cells and/or circulating B cells other peripheral blood lymphocytes (PBLs) are recovered for library construction.
  • PBLs peripheral blood lymphocytes
  • a human antibody gene fragment library biased in favor of anti- VEGF-C clones is obtained by generating an anti-VEGF-C antibody response in transgenic mice carrying a functional human immunoglobulin gene array (and lacking a functional endogenous antibody production system) such that VEGF-C
  • Additional enrichment for anti-VEGF-C reactive cell populations can be obtained by using a suitable screening procedure to isolate B cells expressing VEGF-C-specific membrane bound antibody, e.g., by cell separation using VEGF-C affinity chromatography or adsorption of cells to fluorochrome-labeled VEGF-C followed by flow-activated cell sorting (FACS).
  • FACS flow-activated cell sorting
  • spleen cells and/or B cells or other PBLs from an unimmunized donor provides a better representation of the possible antibody repertoire, and also permits the construction of an antibody library using any animal (human or non-human) species in which VEGF-C is not antigenic.
  • stem cells are harvested from the subject to provide nucleic acids encoding unrearranged antibody gene segments.
  • the immune cells of interest can be obtained from a variety of animal species, such as human, mouse, rat, lagomorpha, luprine, canine, feline, porcine, bovine, equine, and avian species, etc.
  • Nucleic acid encoding antibody variable gene segments are recovered from the cells of interest and amplified.
  • the desired DNA can be obtained by isolating genomic DNA or mRNA from lymphocytes followed by polymerase chain reaction (PCR) with primers matching the 5' and 3' ends of rearranged VH and VL genes as described in Orlandi et ah, Proc. Natl. Acad. Sci. (USA), 86: 3833-3837 (1989), thereby making diverse V gene repertoires for expression.
  • the V genes can be amplified from cDNA and genomic DNA, with back primers at the 5' end of the exon encoding the mature V-domain and forward primers based within the J-segment as described in Orlandi et al. (1989) and in Ward et al, Nature, 341 : 544-546 (1989).
  • back primers can also be based in the leader exon as described in Jones et al, Biotechnol, 9: 88-89 (1991), and forward primers within the constant region as described in Sastry et al, Proc. Natl. Acad. Sci. (USA), 86: 5728-5732 (1989).
  • degeneracy can be used to maximize complementarity.
  • library diversity is maximized by using PCR primers targeted to each V-gene family in order to amplify all available VH and VL arrangements present in the immune cell nucleic acid sample, e.g. as described in the method of Marks et al., J. Mol. Biol, 222: 581-597 (1991) or as described in the method of Orum et al, Nucleic Acids Res., 21 : 4491-4498 (1993).
  • rare restriction sites can be introduced within the PCR primer as a tag at one end as described in Orlandi et al. (1989), or by further PCR amplification with a tagged primer as described in Clackson et al, Nature, 352: 624-628 (1991).
  • Repertoires of synthetically rearranged V genes can be derived in vitro from V gene segments. Most of the human VH-gene segments have been cloned and sequenced (reported in Tomlinson et al, J. Mol. Biol, 227: 776-798 (1992)), and mapped (reported in Matsuda et al, Nature Genet., 3 : 88-94 (1993); these cloned segments (including all the major conformations of the HI and H2 loop) can be used to generate diverse VH gene repertoires with PCR primers encoding H3 loops of diverse sequence and length as described in Hoogenboom and Winter, J. Mol. Biol, 227: 381-388 (1992).
  • VH repertoires can also be made with all the sequence diversity focused in a long H3 loop of a single length as described in Barbas et al, Proc. Natl. Acad. Sci. USA, 89: 4457-4461 (1992).
  • Human VK and ⁇ segments have been cloned and sequenced (reported in Williams and Winter, Eur. J. Immunol, 23: 1456- 1461 (1993)) and can be used to make synthetic light chain repertoires.
  • Synthetic V gene repertoires based on a range of VH and VL folds, and L3 and H3 lengths, will encode antibodies of considerable structural diversity.
  • germline V-gene segments can be rearranged in vitro according to the methods of Hoogenboom and Winter, J. Mol. Biol, 227: 381-388 (1992).
  • Repertoires of antibody fragments can be constructed by combining VH and VL gene repertoires together in several ways. Each repertoire can be created in different vectors, and the vectors recombined in vitro, e.g., as described in Hogrefe et al., Gene, 128: 119-126 (1993), or in vivo by combinatorial infection, e.g., the loxP system described in Waterhouse et al, Nucl. Acids Res., 21 : 2265-2266 (1993). The in vivo recombination approach exploits the two-chain nature of Fab fragments to overcome the limit on library size imposed by E. coli transformation efficiency.
  • Naive VH and VL repertoires are cloned separately, one into a phagemid and the other into a phage vector.
  • the two libraries are then combined by phage infection of phagemid-containing bacteria so that each cell contains a different combination and the library size is limited only by the number of cells present (about
  • the repertoires may be cloned sequentially into the same vector, e.g. as described in Barbas et al., Proc. Natl. Acad. Sci. USA, 88: 7978- 7982 (1991), or assembled together by PCR and then cloned, e.g. as described in Clackson et al., Nature, 352: 624-628 (1991).
  • PCR assembly can also be used to join VH and VL DNAs with DNA encoding a flexible peptide spacer to form single chain Fv (scFv) repertoires.
  • in cell PCR assembly is used to combine VH and VL genes within lymphocytes by PCR and then clone repertoires of linked genes as described in Embleton et al., Nucl. Acids Res., 20: 3831-3837 (1992).
  • the antibodies produced by naive libraries can be of moderate affinity (K ⁇ f 1 of about 10 6 to 10 7 M "1 ), but affinity maturation can also be mimicked in vitro by constructing and reselecting from secondary libraries as described in Winter et al. (1994), supra.
  • mutation can be introduced at random in vitro by using error-prone polymerase (reported in Leung et al., Technique, 1: 11-15 (1989)) in the method of Hawkins et al., J. Mol. Biol., 226: 889-896 (1992) or in the method of Gram et al., Proc. Natl. Acad. Sci USA, 89: 3576-3580 (1992).
  • affinity maturation can be performed by randomly mutating one or more CDRs, e.g. using PCR with primers carrying random sequence spanning the CDR of interest, in selected individual Fv clones and screening for higher affinity clones.
  • WO 9607754 published 14 March 1996) described a method for inducing mutagenesis in a complementarity determining region of an immunoglobulin light chain to create a library of light chain genes.
  • VH or VL domains selected by phage display with repertoires of naturally occurring V domain variants obtained from unimmunized donors and screen for higher affinity in several rounds of chain reshuffling as described in Marks et al., Biotechnol., 10: 779-783 (1992).
  • This technique allows the production of antibodies and antibody fragments with affinities of about 10 "9 M or less.
  • VEGF-C can be used to coat the wells of adsorption plates, expressed on host cells affixed to adsorption plates or used in cell sorting, or conjugated to biotin for capture with streptavidin-coated beads, or used in any other method for panning phage display libraries.
  • the phage library samples are contacted with immobilized VEGF- C under conditions suitable for binding at least a portion of the phage particles with the adsorbent. Normally, the conditions, including pH, ionic strength, temperature and the like are selected to mimic physiological conditions.
  • the phages bound to the solid phase are washed and then eluted by acid, e.g. as described in Barbas et al., Proc. Natl. Acad. Sci USA, 88: 7978-7982 (1991), or by alkali, e.g. as described in Marks et al, J. Mol. Biol, 222: 581-597 (1991), or by VEGF-C antigen competition, e.g.
  • Phages can be enriched 20-1,000-fold in a single round of selection. Moreover, the enriched phages can be grown in bacterial culture and subjected to further rounds of selection.
  • the efficiency of selection depends on many factors, including the kinetics of dissociation during washing, and whether multiple antibody fragments on a single phage can simultaneously engage with antigen.
  • Antibodies with fast dissociation kinetics (and weak binding affinities) can be retained by use of short washes, multivalent phage display and high coating density of antigen in solid phase. The high density not only stabilizes the phage through multivalent interactions, but favors rebinding of phage that has dissociated.
  • the high affinity- binding phages can then be captured by streptavidin-coated paramagnetic beads.
  • streptavidin-coated paramagnetic beads Such "equilibrium capture” allows the antibodies to be selected according to their affinities of binding, with sensitivity that permits isolation of mutant clones with as little as two-fold higher affinity from a great excess of phages with lower affinity.
  • Conditions used in washing phages bound to a solid phase can also be manipulated to discriminate on the basis of dissociation kinetics.
  • Anti-VEGF-C clones may be selected based on activity.
  • the invention provides anti-VEGF-C antibodies that bind to living cells that naturally express VEGF-C.
  • the invention provides anti- VEGF-C antibodies that block the binding between a VEGF-C ligand and VEGF-C, but do not block the binding between a VEGF-C ligand and a second protein.
  • Fv clones corresponding to such anti-VEGF-C antibodies can be selected by (1) isolating anti-VEGF-C clones from a phage library as described above, and optionally amplifying the isolated population of phage clones by growing up the population in a suitable bacterial host; (2) selecting VEGF-C and a second protein against which blocking and non-blocking activity, respectively, is desired; (3) adsorbing the anti- VEGF-C phage clones to immobilized VEGF-C; (4) using an excess of the second protein to elute any undesired clones that recognize VEGF-C-binding determinants which overlap or are shared with the binding determinants of the second protein; and (5) eluting the clones which remain adsorbed following step (4).
  • clones with the desired blocking/non-blocking properties can be further enriched by repeating the selection procedures described herein one or more times.
  • the DNA can be placed into expression vectors, which are then transfected into host cells such as E. coli cells, simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein, to obtain the synthesis of the desired monoclonal antibodies in the recombinant host cells.
  • host cells such as E. coli cells, simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein, to obtain the synthesis of the desired monoclonal antibodies in the recombinant host cells.
  • Review articles on recombinant expression in bacteria of antibody-encoding DNA include Skerra et al. , Curr. Opinion in Immunol., 5: 256 (1993) and Pluckthun, Immunol. Revs, 130: 151 (1992).
  • DNA encoding the Fv clones of the invention can be combined with known DNA sequences encoding heavy chain and/or light chain constant regions (e.g. the appropriate DNA sequences can be obtained from Kabat et al, supra) to form clones encoding full or partial length heavy and/or light chains.
  • constant regions of any isotype can be used for this purpose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • an Fv clone derived from the variable domain DNA of one animal (such as human) species and then fused to constant region DNA of another animal species to form coding sequence(s) for "hybrid," full length heavy chain and/or light chain is included in the definition of "chimeric” and "hybrid” antibody as used herein.
  • an Fv clone derived from human variable DNA is fused to human constant region DNA to form coding sequence(s) for full- or partial-length human heavy and/or light chains.
  • DNA encoding anti-VEGF-C antibody derived from a hybridoma of the invention can also be modified, for example, by substituting the coding sequence for human heavy- and light-chain constant domains in place of homologous murine sequences derived from the hybridoma clone (e.g. as in the method of Morrison et al, Proc. Natl. Acad. Sci. USA, 81 : 6851-6855 (1984)).
  • DNA encoding a hybridoma- or Fv clone-derived antibody or fragment can be further modified by co valently joining to the immunoglobulin coding sequence all or part of the coding sequence for a non-immunoglobulin polypeptide. In this manner, "chimeric" or “hybrid” antibodies are prepared that have the binding specificity of the Fv clone or hybridoma clone-derived antibodies of the invention.
  • Antibodies may also be produced using recombinant methods.
  • nucleic acid encoding the antibody is isolated and inserted into a replicable vector for further cloning
  • DNA encoding the antibody may be readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of the antibody).
  • Many vectors are available.
  • the vector components generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter, and a transcription termination sequence.
  • An antibody of the invention may be produced recombinantly not only directly, but also as a fusion polypeptide with a heterologous polypeptide, which is preferably a signal sequence or other polypeptide having a specific cleavage site at the N-terminus of the mature protein or polypeptide.
  • a heterologous polypeptide which is preferably a signal sequence or other polypeptide having a specific cleavage site at the N-terminus of the mature protein or polypeptide.
  • the heterologous signal sequence selected preferably is one that is recognized and processed (i.e., cleaved by a signal peptidase) by the host cell.
  • the signal sequence is substituted by a prokaryotic signal sequence selected, for example, from the group of the alkaline phosphatase, penicillinase, lpp, or heat-stable enterotoxin II leaders.
  • a prokaryotic signal sequence selected, for example, from the group of the alkaline phosphatase, penicillinase, lpp, or heat-stable enterotoxin II leaders.
  • yeast secretion the native signal sequence may be substituted by, e.g., the yeast invertase leader, a factor leader (including Saccharomyces and Kluyveromyces a-factor leaders), or acid phosphatase leader, the C. albicans glucoamylase leader, or the signal described in WO 90/13646.
  • mammalian signal sequences as well as viral secretory leaders for example, the herpes simplex gD signal, are available.
  • Both expression and cloning vectors contain a nucleic acid sequence that enables the vector to replicate in one or more selected host cells.
  • this sequence is one that enables the vector to replicate independently of the host chromosomal DNA, and includes origins of replication or autonomously replicating sequences.
  • origins of replication or autonomously replicating sequences are well known for a variety of bacteria, yeast, and viruses.
  • the origin of replication from the plasmid pBR322 is suitable for most Gram-negative bacteria, the 2 ⁇ plasmid origin is suitable for yeast, and various viral origins (SV40, polyoma, adenovirus, VSV or BPV) are useful for cloning vectors in mammalian cells.
  • the origin of replication component is not needed for mammalian expression vectors (the SV40 origin may typically be used only because it contains the early promoter).
  • Expression and cloning vectors may contain a selection gene, also termed a selectable marker.
  • selection genes encode proteins that (a) confer resistance to antibiotics or other toxins, e.g., ampicillin, neomycin, methotrexate, or tetracycline, (b) complement auxotrophic deficiencies, or (c) supply critical nutrients not available from complex media, e.g., the gene encoding D-alanine racemase for Bacilli.
  • One example of a selection scheme utilizes a drug to arrest growth of a host cell. Those cells that are successfully transformed with a heterologous gene produce a protein conferring drug resistance and thus survive the selection regimen. Examples of such dominant selection use the drugs neomycin, mycophenolic acid and hygromycin.
  • Suitable selectable markers for mammalian cells are those that enable the identification of cells competent to take up antibody- encoding nucleic acid, such as DHFR, glutamine synthetase (GS), thymidine kinase, metallothionein-I and -II, preferably primate metallothionein genes, adenosine deaminase, ornithine decarboxylase, etc.
  • cells transformed with the DHFR gene are identified by culturing the transformants in a culture medium containing methotrexate (Mtx), a competitive antagonist of DHFR. Under these conditions, the DHFR gene is amplified along with any other co-transformed nucleic acid.
  • Mtx methotrexate
  • a Chinese hamster ovary (CHO) cell line deficient in endogenous DHFR activity e.g., ATCC CRL- 9096 may be used.
  • cells transformed with the GS gene are identified by culturing the transformants in a culture medium containing L-methionine sulfoximine (Msx), an inhibitor of GS. Under these conditions, the GS gene is amplified along with any other co-transformed nucleic acid.
  • the GS selection/amplification system may be used in combination with the DHFR selection/amplification system described above.
  • host cells particularly wild-type hosts that contain endogenous DHFR transformed or co-transformed with DNA sequences encoding an antibody of interest, wild-type DHFR gene, and another selectable marker such as aminoglycoside 3 '-phosphotransferase (APH) can be selected by cell growth in medium containing a selection agent for the selectable marker such as an APH.
  • APH aminoglycoside 3 '-phosphotransferase
  • aminoglycosidic antibiotic e.g., kanamycin, neomycin, or G418. See U.S. Patent No. 4,965,199.
  • a suitable selection gene for use in yeast is the trpl gene present in the yeast plasmid YRp7 (Stinchcomb et ah, Nature, 282:39 (1979)).
  • the trpl gene provides a selection marker for a mutant strain of yeast lacking the ability to grow in tryptophan, for example, ATCC No. 44076 or PEP4-1. Jones, Genetics, 85: 12 (1977).
  • the presence of the trpl lesion in the yeast host cell genome then provides an effective environment for detecting transformation by growth in the absence of tryptophan.
  • ZeM2-deficient yeast strains (ATCC 20,622 or 38,626) are complemented by known plasmids bearing the Leul gene.
  • vectors derived from the 1.6 ⁇ circular plasmid pKDl can be used for transformation of Kluyveromyces yeasts.
  • an expression system for large-scale production of recombinant calf chymosin was reported for K. lactis. Van den Berg, Bio/Technology, 8: 135 (1990).
  • Stable multicopy expression vectors for secretion of mature recombinant human serum albumin by industrial strains of Kluyveromyces have also been disclosed.
  • Expression and cloning vectors generally contain a promoter that is recognized by the host organism and is operably linked to nucleic acid encoding an antibody.
  • Promoters suitable for use with prokaryotic hosts include the phoA promoter , ⁇ -lactamase and lactose promoter systems, alkaline phosphatase promoter, a tryptophan (trp) promoter system, and hybrid promoters such as the tac promoter.
  • trp tryptophan
  • Other known bacterial promoters are suitable. Promoters for use in bacterial systems also will contain a Shine -Dalgarno (S.D.) sequence operably linked to the DNA encoding an antibody.
  • Promoter sequences are known for eukaryotes. Virtually all eukaryotic genes have an AT-rich region located approximately 25 to 30 bases upstream from the site where transcription is initiated. Another sequence found 70 to 80 bases upstream from the start of transcription of many genes is a CNCAAT region where N may be any nucleotide. At the 3' end of most eukaryotic genes is an AATAAA sequence that may be the signal for addition of the poly A tail to the 3' end of the coding sequence. All of these sequences are suitably inserted into eukaryotic expression vectors.
  • suitable promoter sequences for use with yeast hosts include the promoters for 3-phosphoglycerate kinase or other glycolytic enzymes, such as enolase, glyceraldehyde-3 -phosphate dehydrogenase, hexokinase, pyruvate decarboxylase, phosphofructokinase, glucose-6-phosphate isomerase, 3- phosphoglycerate mutase, pyruvate kinase, triosephosphate isomerase,
  • yeast promoters which are inducible promoters having the additional advantage of transcription controlled by growth conditions, are the promoter regions for alcohol dehydrogenase 2, isocytochrome C, acid phosphatase, degradative enzymes associated with nitrogen metabolism, metallothionein, glyceraldehyde-3 -phosphate dehydrogenase, and enzymes responsible for maltose and galactose utilization.
  • Suitable vectors and promoters for use in yeast expression are further described in EP 73,657.
  • Yeast enhancers also are advantageously used with yeast promoters.
  • Antibody transcription from vectors in mammalian host cells can be controlled, for example, by promoters obtained from the genomes of viruses such as polyoma virus, fowlpox virus, adenovirus (such as Adenovirus 2), bovine papilloma virus, avian sarcoma virus, cytomegalovirus, a retrovirus, hepatitis-B virus, Simian Virus 40 (SV40), or from heterologous mammalian promoters, e.g., the actin promoter or an immunoglobulin promoter, from heat-shock promoters, provided such promoters are compatible with the host cell systems.
  • viruses such as polyoma virus, fowlpox virus, adenovirus (such as Adenovirus 2), bovine papilloma virus, avian sarcoma virus, cytomegalovirus, a retrovirus, hepatitis-B virus, Simian Virus 40 (SV40), or from hetero
  • the early and late promoters of the SV40 virus are conveniently obtained as an SV40 restriction fragment that also contains the SV40 viral origin of replication.
  • the immediate early promoter of the human cytomegalovirus is conveniently obtained as a Hindlll E restriction fragment.
  • a system for expressing DNA in mammalian hosts using the bovine papilloma virus as a vector is disclosed in U.S. Patent No. 4,419,446. A modification of this system is described in U.S. Patent No. 4,601,978.
  • Enhancer sequences are now known from mammalian genes (globin, elastase, albumin, a-fetoprotein, and insulin). Typically, however, one will use an enhancer from a eukaryotic cell virus. Examples include the SV40 enhancer on the late side of the replication origin (bp 100-270), the cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and adenovirus enhancers.
  • the enhancer may be spliced into the vector at a position 5 ' or 3' to the antibody-encoding sequence, but is preferably located at a site 5' from the promoter.
  • Expression vectors used in eukaryotic host cells will also contain sequences necessary for the termination of transcription and for stabilizing the mRNA. Such sequences are commonly available from the 5' and, occasionally 3', untranslated regions of eukaryotic or viral DNAs or cDNAs. These regions contain nucleotide segments transcribed as polyadenylated fragments in the untranslated portion of the mRNA encoding antibody.
  • One useful transcription termination component is the bovine growth hormone polyadenylation region. See W094/11026 and the expression vector disclosed therein.
  • Suitable host cells for cloning or expressing the DNA in the vectors herein are the prokaryote, yeast, or higher eukaryote cells described above.
  • Suitable prokaryotes for this purpose include eubacteria, such as Gram-negative or Gram- positive organisms, for example, Enterobacteriaceae such as Escherichia, e.g., E. coli, Enterobacter, Erwinia, Klebsiella, Proteus, Salmonella, e.g., Salmonella
  • E. coli cloning host is E. coli 294 (ATCC 31,446), although other strains such as E. coli B, E. coli XI 776 (ATCC 31 ,537), and E. coli W3110 (ATCC 27,325) are suitable. These examples are illustrative rather than limiting.
  • Full length antibody, antibody fusion proteins, and antibody fragments can be produced in bacteria, in particular when glycosylation and Fc effector function are not needed, such as when the therapeutic antibody is conjugated to a cytotoxic agent (e.g., a toxin) that by itself shows effectiveness in tumor cell destruction.
  • a cytotoxic agent e.g., a toxin
  • Full length antibodies have greater half life in circulation. Production in E. coli is faster and more cost efficient.
  • For expression of antibody fragments and polypeptides in bacteria see, e.g., U.S. 5,648,237 (Carter et. al), U.S. 5,789,199 (Joly et al), U.S.
  • eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for antibody- encoding vectors.
  • Saccharomyces cerevisiae or common baker's yeast, is the most commonly used among lower eukaryotic host microorganisms.
  • Kluyveromyces hosts such as, e.g., K. lactis, K. fragilis (ATCC 12,424), K. bulgaricus (ATCC 16,045), K. wickeramii (ATCC 24,178), K. waltii (ATCC 56,500), K. drosophilarum (ATCC 36,906), K .
  • thermotolerans and K. marxianus
  • yarrowia EP 402,226
  • Pichia pastoris EP 183,070
  • Candida Trichoderma reesia
  • Neurospora crassa Neurospora crassa
  • Schwanniomyces such as Schwanniomyces occidentalis
  • filamentous fungi such as, e.g., Neurospora, Penicillium, Tolypocladium, and Aspergillus hosts such as A. nidulans and A. niger.
  • yeasts and filamentous fungi for the production of therapeutic proteins, see, e.g., Gerngross, Nat. Biotech. 22: 1409-1414 (2004).
  • glycosylation pathways have been "humanized,” resulting in the production of an antibody with a partially or fully human glycosylation pattern. See, e.g., Li et al., Nat. Biotech. 24:210-215 (2006) (describing humanization of the glycosylation pathway in Pichia pastoris); and Gerngross et al., supra.
  • Suitable host cells for the expression of glycosylated antibody are also derived from multicellular organisms (invertebrates and vertebrates). Examples of invertebrate cells include plant and insect cells. Numerous baculoviral strains and variants and corresponding permissive insect host cells from hosts such as Spodoptera frugiperda (caterpillar), Aedes aegypti (mosquito), Aedes albopictus (mosquito), Drosophila melanogaster (fruitfly), and Bombyx mori have been identified. A variety of viral strains for transfection are publicly available, e.g., the L-l variant of
  • Autographa calif ornica NPV and the Bm-5 strain of Bombyx mori NPV, and such viruses may be used as the virus herein according to the present invention, particularly for transfection of Spodoptera frugiperda cells.
  • Plant cell cultures of cotton, corn, potato, soybean, petunia, tomato, duckweed (Lemnaceae), alfalfa (M. truncatula), and tobacco can also be utilized as hosts. See, e.g., US Patent Nos. 5,959,177, 6,040,498, 6,420,548, 7,125,978, and 6,417,429 (describing PLANTIBODIESTM technology for producing antibodies in transgenic plants).
  • Vertebrate cells may be used as hosts, and propagation of vertebrate cells in culture (tissue culture) has become a routine procedure.
  • useful mammalian host cell lines are monkey kidney CVl line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (293 or 293 cells subcloned for growth in suspension culture, Graham et al, J. Gen Virol. 36:59 (1977)) ; baby hamster kidney cells (BHK, ATCC CCL 10); mouse Sertoli cells (TM4, Mather, Biol. Reprod.
  • monkey kidney cells (CVl ATCC CCL 70); African green monkey kidney cells (VERO-76, ATCC CRL-1587); human cervical carcinoma cells (HELA, ATCC CCL 2); canine kidney cells (MDCK, ATCC CCL 34); buffalo rat liver cells (BRL 3 A, ATCC CRL 1442); human lung cells (W138, ATCC CCL 75); human liver cells (Hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI cells (Mather et al, Annals N. Y. Acad. Sci. 383:44-68 (1982)); MRC 5 cells; FS4 cells; and a human hepatoma line (Hep G2).
  • CHO Chinese hamster ovary
  • DHFR " CHO cells (Urlaub et al, Proc. Natl. Acad. Sci. USA 77:4216 (1980)); and myeloma cell lines such as NSO and Sp2/0.
  • CHO Chinese hamster ovary
  • myeloma cell lines such as NSO and Sp2/0.
  • Yazaki and Wu Methods in Molecular Biology, Vol. 248 (B.K.C. Lo, ed., Humana Press, Totowa, NJ, 2003), pp. 255-268.
  • Host cells are transformed with the above-described expression or cloning vectors for antibody production and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences.
  • the host cells used to produce an antibody of this invention may be cultured in a variety of media.
  • Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPMI-1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing the host cells.
  • the antibody can be produced intracellularly, in the periplasmic space, or directly secreted into the medium. If the antibody is produced intracellularly, as a first step, the particulate debris, either host cells or lysed fragments, are removed, for example, by centrifugation or ultrafiltration. Carter et al., Bio/Technology 10: 163-167 (1992) describe a procedure for isolating antibodies which are secreted to the periplasmic space of E. coli. Briefly, cell paste is thawed in the presence of sodium acetate (pH 3.5), EDTA, and
  • phenylmethylsulfonylfluoride over about 30 min. Cell debris can be removed by centrifugation.
  • supematants from such expression systems are generally first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration unit.
  • a protease inhibitor such as PMSF may be included in any of the foregoing steps to inhibit proteolysis and antibiotics may be included to prevent the growth of adventitious contaminants.
  • the antibody composition prepared from the cells can be purified using, for example, hydroxylapatite chromatography, hydrophobic interaction chromatography, gel electrophoresis, dialysis, and affinity chromatography, with affinity chromatography being among one of the typically preferred purification steps.
  • affinity chromatography is among one of the typically preferred purification steps.
  • the suitability of protein A as an affinity ligand depends on the species and isotype of any immunoglobulin Fc domain that is present in the antibody. Protein A can be used to purify antibodies that are based on human ⁇ , ⁇ 2, or ⁇ 4 heavy chains (Lindmark et al., J. Immunol. Meth. 62: 1-13 (1983)).
  • Protein G is recommended for all mouse isotypes and for human ⁇ 3 (Guss et al, EMBO J. 5: 15671575 (1986)).
  • the matrix to which the affinity ligand is attached is most often agarose, but other matrices are available.
  • Mechanically stable matrices such as controlled pore glass or
  • poly(styrenedivinyl)benzene allow for faster flow rates and shorter processing times than can be achieved with agarose.
  • the Bakerbond ABXTMresin J. T. Baker, Phillipsburg, NJ
  • Other techniques for protein purification such as fractionation on an ion-exchange column, ethanol precipitation, Reverse Phase HPLC, chromatography on silica, chromatography on heparin SEPHAROSETM chromatography on an anion or cation exchange resin (such as a polyaspartic acid column), chromatofocusing, SDS-PAGE, and ammonium sulfate precipitation are also available depending on the antibody to be recovered.
  • the mixture comprising the antibody of interest and contaminants may be subjected to low pH hydrophobic interaction chromatography using an elution buffer at a pH between about 2.5-4.5, preferably performed at low salt concentrations (e.g., from about 0- 0.25M salt).
  • the antibody composition will be formulated, dosed, and administered in a fashion consistent with good medical practice.
  • Factors for consideration in this context include, but not limited to, the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners.
  • the appropriate dosage of an antibody of the invention when used alone or in combination with one or more other additional therapeutic agents) will depend on the type of disease to be treated, the type of antibody, the severity and course of the disease, whether the antibody is
  • the antibody is suitably administered to the patient at one time or over a series of treatments.
  • compositions herein may also contain more than one active compound as necessary for the particular indication being treated, preferably those with complementary activities that do not adversely affect each other. Such molecules are suitably present in combination in amounts that are effective for the purpose intended.
  • the "therapeutically effective amount" of the antibody to be administered will be governed by considerations discussed above, and is the minimum amount necessary to prevent, ameliorate, or treat a disease or disorder.
  • the antibody need not be, but is optionally formulated with one or more agents currently used to prevent or treat the disorder in question.
  • the effective amount of such other agents depends on the amount of antibody present in the formulation, the type of disorder or treatment, and other factors discussed above. These are generally used in the same dosages and with administration routes as used hereinbefore or about from 1 to 99% of the heretofore employed dosages.
  • alleviation or treatment of a disease or disorder involves the lessening of one or more symptoms or medical problems associated with the disease or disorder.
  • the therapeutically effective amount of the drug can accomplish one or a combination of the following: reduce the number of cancer cells; reduce the tumor size; inhibit (i.e., to decrease to some extent and/or stop) cancer cell infiltration into peripheral organs; inhibit tumor metastasis; inhibit, to some extent, tumor growth; and/or relieve to some extent one or more of the symptoms associated with the cancer.
  • the drug may prevent growth and/or kill existing cancer cells, it may be cytostatic and/or cytotoxic.
  • a composition of this invention can be used to prevent the onset or reoccurrence of the disease or disorder in a subject or mammal.
  • about 1 ⁇ g/kg to 50 mg/kg (e.g., 0.1-20 mg/kg) of antibody is an initial candidate dosage for administration to the patient, whether, for example, by one or more separate administrations, or by continuous infusion.
  • about 1 ⁇ g/kg to 15 mg/kg (e.g. 0.1 mg/kg- 10 mg/kg) of antibody is an initial candidate dosage for administration to the patient.
  • a typical daily dosage might range from about 1 ⁇ g/kg to 100 mg/kg or more, depending on the factors mentioned above. For repeated administrations over several days or longer, depending on the condition, the treatment is sustained until a desired suppression of disease symptoms occurs.
  • One exemplary dosage of the antibody would be in the range from about 0.05 mg/kg to about 15 mg/kg.
  • one or more doses of about 0.5 mg/kg, 1.0 mg/kg, 2.0 mg/kg, 3.0 mg/kg, 4.0 mg/kg, 5.0 mg/kg, 6.0 mg/kg, 7.0 mg/kg, 7.5 mg/kg, 8.0 mg/kg, 9.0 mg/kg, 10 mg/kg or 15 mg/kg (or any combination thereof) may be administered to the patient.
  • Such doses may be administered intermittently, e.g. every day, every three days, every week or every two to three weeks (e.g. such that the patient receives from about two to about twenty, or e.g. about six doses of the antibody).
  • dose of about 10 mg/kg is administered every three days.
  • An initial higher loading dose, followed by one or more lower doses may be administered.
  • an exemplary dosing regimen comprises administering an initial loading dose of about 4 mg/kg, followed by a weekly maintenance dose of about 2 mg/kg of the antibody.
  • other dosage regimens may be useful.
  • dosing regimens discussed herein are used in combination with anti-VEGF antibody and/or a chemotherapy regimen as the first line therapy for treating metastatic colorectal cancer. In some aspects, the
  • chemotherapy regimen involves the traditional high-dose intermittent administration.
  • the chemotherapeutic agents are administered using smaller and more frequent doses without scheduled breaks ("metronomic chemotherapy").
  • An antibody of the invention (and any additional therapeutic agent or adjuvant) can be administered by any suitable means, including parenteral, subcutaneous, intraperitoneal, intrapulmonary, and intranasal, and, if desired for local treatment, intralesional administration.
  • Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration.
  • the antibody is suitably administered by pulse infusion, particularly with declining doses of the antibody. Dosing can be by any suitable route, e.g. by injections, such as intravenous or subcutaneous injections, depending in part on whether the
  • administration is brief or chronic.
  • the location of the binding target of an antibody of the invention may be taken into consideration in preparation and administration of the antibody.
  • certain embodiments of the invention provide for the antibody to traverse the blood-brain barrier.
  • Several art- known approaches exist for transporting molecules across the blood-brain barrier including, but not limited to, physical methods, lipid-based methods, stem cell-based methods, and receptor and channel-based methods.
  • Circumvention methods include, but are not limited to, direct injection into the brain (see, e.g., Papanastassiou et al, Gene Therapy 9: 398-406 (2002)), interstitial infusion/convection-enhanced delivery (see, e.g., Bobo et al, Proc. Natl. Acad. Sci. USA 91 : 2076-2080 (1994)), and implanting a delivery device in the brain (see, e.g., Gill et al, Nature Med. 9: 589-595 (2003); and Gliadel WafersTM, Guildford Pharmaceutical).
  • Methods of creating openings in the barrier include, but are not limited to, ultrasound (see, e.g., U.S.
  • Patent Publication No. 2002/0038086 osmotic pressure (e.g., by administration of hypertonic mannitol (Neuwelt, E. A., Implication of the Blood-Brain Barrier and its Manipulation, Vols 1 & 2, Plenum Press, N.Y. (1989)), permeabilization by, e.g., bradykinin or permeabilizer A-7 (see, e.g., U.S. Patent Nos. 5,112,596, 5,268,164, 5,506,206, and 5,686,416), and transfection of neurons that straddle the blood-brain barrier with vectors containing genes encoding the antibody (see, e.g., U.S. Patent Publication No. 2003/0083299).
  • Lipid-based methods of transporting an antibody across the blood- brain barrier include, but are not limited to, encapsulating the antibody in liposomes that are coupled to antibody binding fragments that bind to receptors on the vascular endothelium of the blood-brain barrier (see, e.g., U.S. Patent Application Publication No. 20020025313), and coating the antibody in low-density lipoprotein particles (see, e.g., U.S. Patent Application Publication No. 20040204354) or apolipoprotein E (see, e.g., U.S. Patent Application Publication No. 20040131692).
  • NPCs neural progenitor cells
  • Receptor and channel-based methods of transporting an antibody across the blood-brain barrier include, but are not limited to, using glucocorticoid blockers to increase permeability of the blood-brain barrier (see, e.g., U.S. Patent Application Publication Nos. 2002/0065259, 2003/0162695, and 2005/0124533); activating potassium channels (see, e.g., U.S. Patent Application Publication No. 2005/0089473), inhibiting ABC drug transporters (see, e.g., U.S. Patent Application Publication No.
  • compositions comprising an antibody of the invention are prepared for storage by mixing the antibody having the desired degree of purity with optional physiologically acceptable carriers, excipients or stabilizers (Remington: The Science and Practice of Pharmacy 20th edition (2000)), in the form of aqueous solutions, lyophilized or other dried formulations.
  • Acceptable carriers, excipients, or stabilizers are nontoxic to recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate, histidine and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride;
  • benzalkonium chloride benzethonium chloride
  • phenol butyl or benzyl alcohol
  • alkyl parabens such as methyl or propyl paraben
  • catechol resorcinol
  • cyclohexanol 3- pentanol
  • m-cresol low molecular weight polypeptides
  • proteins such as serum albumin, gelatin, or immunoglobulins
  • hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose or sorbitol; salt-forming counter-ions such as sodium; metal complexes (e.g., Zn-protein complexes); and/or non-ionic surfactants such as TWEENTM, PLURONICSTM or polyethylene glycol (PEG).
  • amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine
  • monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins such as EDTA
  • sugars such as sucrose, mannitol, trehalose
  • the active ingredients may also be entrapped in microcapsule prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsule and poly- (methylmethacylate) microcapsule, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules) or in macroemulsions.
  • colloidal drug delivery systems for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules
  • formulations to be used for in vivo administration must be sterile. This is readily accomplished by filtration through sterile filtration membranes.
  • Sustained-release preparations may be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the immunoglobulin of the invention, which matrices are in the form of shaped articles, e.g., films, or microcapsule. Examples of sustained-release matrices include polyesters, hydrogels (for example, poly(2- hydroxyethyl-methacrylate), or poly(vinylalcohol)), polylactides (U.S. Pat. No.
  • copolymers of L-glutamic acid and ⁇ ethyl-L-glutamate copolymers of L-glutamic acid and ⁇ ethyl-L-glutamate, non-degradable ethylene-vinyl acetate, degradable lactic acid-glycolic acid copolymers such as the LUPRON DEPOTTM (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(-)-3-hydroxybutyric acid. While polymers such as ethylene-vinyl acetate and lactic acid-glycolic acid enable release of molecules for over 100 days, certain hydrogels release proteins for shorter time periods.
  • encapsulated immunoglobulins When encapsulated immunoglobulins remain in the body for a long time, they may denature or aggregate as a result of exposure to moisture at 37°C, resulting in a loss of biological activity and possible changes in immunogenicity. Rational strategies can be devised for stabilization depending on the mechanism involved. For example, if the aggregation mechanism is discovered to be intermolecular S-S bond formation through thio-disulfide interchange, stabilization may be achieved by modifying sulfhydryl residues, lyophilizing from acidic solutions, controlling moisture content, using appropriate additives, and developing specific polymer matrix compositions.
  • the invention further provides methods, kits and articles of manufacture for modulating (e.g., inhibiting) processes involved in
  • lympthangiogenesis and angiogenesis and for use in targeting pathological conditions associated with lymphangiogenesis and angiogenesis, such as cancer.
  • Anti-angiogenic therapy in relationship to cancer is a cancer treatment strategy aimed at inhibiting the development of tumor blood vessels required for providing nutrients to support tumor growth. Because angiogenesis is involved in both primary tumor growth and metastasis, the antiangiogenic treatment provided by the invention is capable of inhibiting the neoplastic growth of tumor at the primary site as well as preventing metastasis of tumors at the secondary sites, therefore allowing attack of the tumors by other therapeutics.
  • the invention encompasses methods for inhibiting angiogenesis using an effective amount of a VEGF-C antagonist (such as an anti- VEGF-C antibody or a VEGF-C immunoadhesin) to inhibit VEGF-C activation of VEGF-C receptors (such as VEGFR3 and VEGFR2).
  • a VEGF-C antagonist such as an anti- VEGF-C antibody or a VEGF-C immunoadhesin
  • the invention provides methods for inhibiting lymphangiogenesis comprising administering an effective amount of a VEGF-C antagonist to a subject in need of such treatment.
  • the VEGF-C antagonist is capable of inhibiting LEC endothelial cell migration, proliferation and/or inhibiting LEC sprouting.
  • the invention provides methods for inhibiting LEC endothelial cell proliferation and/or inhibiting LEC endothelial cell migration comprising administering an effective amount of a VEGF-C antagonist and a VEGF- A antagonist to a subject in need of such treatment.
  • the VEGF-C antagonist is anti-VEGF-C antibody and the VEGF-A antagonist is anti-VEGF-A antibody.
  • the anti-VEGF-A antibody is bevacizumab.
  • An antibody of the invention may be used in, for example, in vitro, ex vivo, and in vivo therapeutic methods.
  • the invention provides methods for treating or preventing a tumor, a cancer, and/or a cell proliferative disorder (e.g., disorder associated with increased expression and/or activity of VEGF-C) comprising administering an effective amount of an anti-VEGF-C antibody to a subject in need of such treatment.
  • a cell proliferative disorder e.g., disorder associated with increased expression and/or activity of VEGF-C
  • the invention provides methods for reducing, inhibiting, blocking, or preventing growth of a tumor or cancer, the methods comprising administering an effective amount of an anti-VEGF-C antibody to a subject in need of such treatment.
  • the invention provides methods for inhibiting angiogenesis comprising administering an effective amount of an anti-VEGF-C antibody to a subject in need of such treatment.
  • the invention provides methods for treating a pathological condition associated with angiogenesis comprising administering an effective amount of an anti-VEGF-C antibody to a subject in need of such treatment.
  • the pathological condition associated with angiogenesis is a tumor, a cancer, and/or a cell proliferative disorder.
  • an antibody of the invention can be administered to a human for therapeutic purposes.
  • an antibody of the invention is used in a method for binding VEGF-C in an individual suffering from a disorder associated with increased VEGF-C expression and/or activity, the method comprising administering to the individual the antibody such that VEGF-C in the individual is bound.
  • the VEGF-C is human VEGF-C
  • the individual is a human individual.
  • the individual can be a mammal expressing VEGF- C to which an antibody of the invention binds.
  • the individual can be a mammal into which VEGF-C has been introduced (e.g., by administration of VEGF-C or by expression of a transgene encoding VEGF-C).
  • the antibodies of the invention can bind VEGF-C from species other than human. Accordingly, the antibodies of the invention can be used to bind specific antigen activity, e.g., in a cell culture containing the antigen, in human subjects or in other mammalian subjects having the antigen with which an antibody of the invention cross-reacts (e.g., chimpanzee, baboon, marmoset, cynomolgus and rhesus, pig or mouse). In one embodiment, the antibody of the invention can be used for inhibiting antigen activities by contacting the antibody with the antigen such that antigen activity is inhibited.
  • the antigen is a human protein molecule.
  • an antibody of the invention can be administered to a non-human mammal expressing VEGF-C with which the antibody cross-reacts (e.g., a primate, pig, rat, or mouse) for veterinary purposes or as an animal model of human disease.
  • a non-human mammal expressing VEGF-C with which the antibody cross-reacts e.g., a primate, pig, rat, or mouse
  • animal models may be useful for evaluating the therapeutic efficacy of antibodies of the invention (e.g., testing of dosages and time courses of administration).
  • the antibodies of the invention can be used to treat, inhibit, delay progression of, prevent/delay recurrence of, ameliorate, or prevent diseases, disorders or conditions associated with expression and/or activity of one or more antigen molecules.
  • the present invention also encompasses the prevention and treatment of tumoral lymphangiogenesis, the prevention and treatment of tumor metastasis and anti-angiogenic cancer therapy, a novel cancer treatment strategy aimed at inhibiting the development of tumor blood vessels required for providing nutrients to support tumor growth.
  • the invention specifically includes inhibiting the neoplastic growth of tumor at the primary site as well as preventing and/or treating metastasis of tumors at the secondary sites, therefore allowing attack of the tumors by other therapeutics.
  • cancer to be treated (including prevention) herein include, but are not limited to, cancers provided herein under "Definitions,” such as carcinoma, lymphoma, blastoma, sarcoma, and leukemia or lymphoid malignancies.
  • cancers include, but not limited to, squamous cell cancer (e.g., epithelial squamous cell cancer), lung cancer including small-cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung and squamous carcinoma of the lung, cancer of the peritoneum, hepatocellular cancer, gastric or stomach cancer including gastrointestinal cancer and gastrointestinal stromal cancer, pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, cancer of the urinary tract, hepatoma, breast cancer, colon cancer, rectal cancer, colorectal cancer, endometrial or uterine carcinoma, salivary gland carcinoma, kidney or renal cancer, prostate cancer, vulval cancer, thyroid cancer, hepatic carcinoma, anal carcinoma, penile carcinoma, melanoma, superficial spreading melanoma, lentigo maligna melanoma, acral lentiginous melanomas, nodular melan
  • Macroglobulinemia may be chronic lymphocytic leukemia (CLL); acute lymphoblastic leukemia (ALL); hairy cell leukemia; chronic myeloblastic leukemia; and post- transplant lymphoproliferative disorder (PTLD), as well as abnormal vascular proliferation associated with phakomatoses, edema (such as that associated with brain tumors), Meigs' syndrome, brain, as well as head and neck cancer, and associated metastases.
  • CLL chronic lymphocytic leukemia
  • ALL acute lymphoblastic leukemia
  • PTLD post- transplant lymphoproliferative disorder
  • cancers that are amenable to treatment by the antibodies of the invention include breast cancer, colorectal cancer, rectal cancer, non- small cell lung cancer, glioblastoma, non-Hodgkins lymphoma (NHL), renal cell cancer, prostate cancer, liver cancer, pancreatic cancer, soft-tissue sarcoma, kaposi's sarcoma, carcinoid carcinoma, head and neck cancer, ovarian cancer, mesothelioma, and multiple myeloma.
  • breast cancer colorectal cancer, rectal cancer, non- small cell lung cancer, glioblastoma, non-Hodgkins lymphoma (NHL), renal cell cancer, prostate cancer, liver cancer, pancreatic cancer, soft-tissue sarcoma, kaposi's sarcoma, carcinoid carcinoma, head and neck cancer, ovarian cancer, mesothelioma, and multiple myeloma.
  • the cancer is selected from the group consisting of small cell lung cancer, glioblastoma, neuroblastomas, melanoma, breast carcinoma, gastric cancer, colorectal cancer (CRC), and hepatocellular carcinoma. Yet, in some embodiments, the cancer is selected from the group consisting of non- small cell lung cancer, colorectal cancer, renal cancer, ovarian cancer, glioblastoma and breast carcinoma, including metastatic forms of those cancers.
  • the invention further specifically includes preventing and/or treating non-neoplastic conditions.
  • Non-neoplastic conditions that are amenable to treatment with antagonists useful in the invention include, but are not limited to, e.g., undesired or aberrant hypertrophy, arthritis, rheumatoid arthritis (RA), psoriasis, psoriatic plaques, sarcoidosis, atherosclerosis, atherosclerotic plaques, edema from myocardial infarction, diabetic and other proliferative retinopathies including retinopathy of prematurity, retrolental fibroplasia, neovascular glaucoma, age-related macular degeneration, diabetic macular edema, corneal neovascularization, corneal graft neovascularization, corneal graft rejection, retinal/choroidal neovascularization, neovascularization of the angle (rubeosis), ocular n
  • disorders to be treated with a VEGF-C antagonist include an epithelial or cardiac disorder.
  • an immunoconjugate of the invention in place of or in addition to the anti-VEGF-C antibody.
  • an immunoconjugate comprising an antibody conjugated with one or more cytotoxic agent(s) is administered to the patient.
  • the immunoconjugate and/or antigen to which it is bound is/are internalized by the cell, resulting in increased therapeutic efficacy of the immunoconjugate in killing the target cell to which it binds.
  • the cytotoxic agent targets or interferes with nucleic acid in the target cell.
  • the cytotoxic agent targets or interferes with microtubule polymerization. Examples of such cytotoxic agents include any of the
  • chemotherapeutic agents noted herein such as a maytansinoid, auristatin, dolastatin, or a calicheamicin
  • a radioactive isotope such as a maytansinoid, auristatin, dolastatin, or a calicheamicin
  • a radioactive isotope such as a ribonuclease or a DNA endonuclease.
  • Antibodies of the invention can be used either alone or in combination with other compositions in a therapy.
  • an antibody of the invention may be co-administered with at least one additional therapeutic agent and/or adjuvant
  • an anti-VEGF-C antibody of the invention may be coadministered with another antibody (e.g., anti-VEGF antibody), chemotherapeutic agent(s) (including cocktails of chemotherapeutic agents), other cytotoxic agent(s), anti-angiogenic agent(s), cytokines, and/or growth inhibitory agent(s).
  • an antibody of the invention inhibits tumor growth, it may be particularly desirable to combine it with one or more other therapeutic agent(s) which also inhibits tumor growth, e.g., anti-angiogenic agents and/or
  • the anti-VEGF-C antibodies and anti-cancer agents are suitable for the same or similar diseases to block or reduce a pathological disorder such as a tumor, a cancer or a cell proliferative disorder.
  • anti-VEGF-C antibodies may be used in combinations with anti-cancer therapeutics or anti-neovascularization therapeutics to treat various neoplastic or non-neoplastic conditions.
  • anti-VEGF-C antibodies may be used in combinations with other inhibitors of VEGF-C.
  • the neoplastic or non-neoplastic condition is characterized by pathological disorder associated with aberrant or undesired angiogenesis.
  • the anti-cancer agent is an anti-angiogenic agent.
  • the anti-VEGF-C antibody of the invention is used in combination with an anti-VEGF antibody to generate additive or synergistic effects.
  • two or more antibodies binding the same or two or more different antigens disclosed herein can be co-administered to the patient.
  • anti-VEGF antibodies include those that bind to the same epitope as the anti-hVEGF antibody A4.6.1.
  • the anti- VEGF antibody is bevacizumab or ranibizumab. Sometimes, it may be beneficial to also administer one or more cytokines to the patient.
  • a VEGF-C antagonist is used in combination with an anti-angiogenic agent such as anti-VEGF neutralizing antibody (or fragment) and/or another VEGF antagonist and/or a VEGF receptor antagonist including, but not limited to, for example, soluble VEGF receptor (e.g., VEGFR-1, VEGFR-2, VEGFR- 3, neuropillins (e.g., NRP1, NRP2)) fragments, aptamers capable of blocking VEGF or VEGFR, neutralizing anti-VEGFR antibodies, low molecule weight inhibitors of VEGFR tyrosine kinases (RTK), antisense strategies for VEGF, ribozymes against VEGF or VEGF receptors, antagonist variants of VEGF; and any combinations thereof.
  • an anti-angiogenic agent such as anti-VEGF neutralizing antibody (or fragment) and/or another VEGF antagonist and/or a VEGF receptor antagonist including, but not limited to, for example, soluble VEGF receptor (e.g
  • anti-angiogenic agent is a VEGF antagonist.
  • VEGF antagonist is an anti-VEGF antibody.
  • anti-VEGF antibody is bevacizumab (AVASTIN ® , Genentech, Inc., South San Francisco, CA).
  • anti-VEGF antibody is B20-4.1.1 described in US Patent Publication No. 2009/0142343.
  • anti-angiogenic agent is an anti-NRPl antibody as described in PCT Publication No. WO2007056470, the entire disclosure of which is expressly incorporated herein by reference.
  • anti-angiogenic agent is an anti-NRP2 antibody as described in PCT Application No.
  • anti-angiogenic agent is an antibody described in PCT Application No. PCT/US2007/069185, the entire disclosure of which is expressly incorporated herein by reference.
  • the anti-VEGF antibody of the invention can be used in combination with small molecule receptor tyrosine kinase inhibitors (RTKIs) that target one or more tyrosine kinase receptors such as VEGF receptors, FGF receptors, EGF receptors and PDGF receptors.
  • RTKIs small molecule receptor tyrosine kinase inhibitors
  • RTKIs are known in the art, including, but are not limited to, vatalanib (PTK787), erlotinib (TARCEVA ® ), OSI-7904, ZD6474 (ZACTIMA ® ), ZD6126 (ANG453), ZD 1839, sunitinib (SUTENT ® ), semaxanib (SU5416), AMG706, AGO 13736, Imatinib (GLEEVEC ® ), MLN-518, CEP-701, PKC- 412, Lapatinib (GSK572016), VELCADE ® , AZD2171, sorafenib (NEXAVAR ® ), XL880, and CHIR-265.
  • Other therapeutic agents useful for combination tumor therapy with the antibody of the invention include antagonist of other factors that are involved in tumor growth, such as EGFR, ErbB2 (also known as Her2) ErbB3, ErbB4, or TNF.
  • two or more angiogenesis inhibitors may optionally be co-administered to the patient in addition to VEGF-C antagonist and other agent.
  • one or more additional therapeutic agents e.g., anticancer agents, can be administered in combination with VEGF-C antagonist, the VEGF antagonist, and an anti-angiogenic agent.
  • other therapeutic agents useful for combination tumor therapy with an anti-VEGF-C antibody include other cancer therapies, e.g., surgery and radiological treatments (e.g. external beam irradiation or therapy with a radioactive labeled agent, such as an antibody.
  • the patient may receive an antibody of the invention combined with radiation therapy.
  • the administration of the VEGF-C antagonist and the other therapeutic agent can be done simultaneously, e.g., as a single composition or as two or more distinct compositions using the same or different administration routes. Alternatively, or additionally, the administration can be done sequentially, in any order. For example, the anti-cancer agent may be administered first, followed by the VEGF-C antagonist. Alternatively, or additionally, the steps can be performed as a combination of both sequentially and simultaneously, in any order. In certain embodiments, intervals ranging from minutes to days, to weeks to months, can be present between the administrations of the two or more compositions.
  • VEGF-C antagonists The effective amounts of therapeutic agents administered in combination with a VEGF-C antagonist will be at the physician's or veterinarian's discretion. Dosage administration and adjustment is done to achieve maximal management of the conditions to be treated. The dose will additionally depend on such factors as the type of therapeutic agent to be used and the specific patient being treated. Suitable dosages for the anti-cancer agent are those presently used and can be lowered due to the combined action (synergy) of the anti-cancer agent and the VEGF- C antagonist. In certain embodiments, the combination of the inhibitors potentiates the efficacy of a single inhibitor. The term "potentiate" refers to an improvement in the efficacy of a therapeutic agent at its common or approved dose. See also the section entitled Pharmaceutical Formulations and Dosages herein.
  • the invention provides a method of treating a disorder (such as a tumor, a cancer, or a cell proliferative disorder) by administering effective amounts of an antagonist of VEGF-C and/or an angiogenesis inhibitor(s) and one or more chemotherapeutic agents.
  • a disorder such as a tumor, a cancer, or a cell proliferative disorder
  • an antagonist of VEGF-C and/or an angiogenesis inhibitor(s) and one or more chemotherapeutic agents may be used in the combined treatment methods of the invention.
  • An exemplary and non- limiting list of chemotherapeutic agents contemplated is provided herein under "Definitions.”
  • chemotherapeutic agent can be done simultaneously, e.g., as a single composition or as two or more distinct compositions, using the same or different administration routes. Alternatively, or additionally, the administration can be done sequentially, in any order. Alternatively, or additionally, the steps can be performed as a
  • intervals ranging from minutes to days, to weeks to months can be present between the administrations of the two or more compositions.
  • the chemotherapeutic agent may be administered first, followed by the VEGF-C antagonist.
  • simultaneous administration or administration of the VEGF-C antagonist first is also contemplated.
  • the invention provides methods comprising administration of a VEGF-C antagonist (such as an anti- VEGF-C antibody), followed by administration of a chemotherapeutic agent.
  • intervals ranging from minutes to days, to weeks to months can be present between the administrations of the two or more compositions.
  • chemotherapeutic agents will be generally around those already employed in clinical therapies wherein the chemotherapeutics are administered alone or in combination with other chemotherapeutics. Variation in dosage will likely occur depending on the condition being treated. The physician administering treatment will be able to determine the appropriate dose for the individual subject. Relapse tumor growth
  • the invention also provides methods and compositions for inhibiting or preventing relapse tumor growth or relapse cancer cell growth.
  • Relapse tumor growth or relapse cancer cell growth is used to describe a condition in which patients undergoing or treated with one or more currently available therapies (e.g., cancer therapies, such as chemotherapy, radiation therapy, surgery, hormonal therapy and/or biological therapy/immunotherapy, anti-VEGF antibody therapy, particularly a standard therapeutic regimen for the particular cancer) is not clinically adequate to treat the patients or the patients are no longer receiving any beneficial effect from the therapy such that these patients need additional effective therapy.
  • cancer therapies such as chemotherapy, radiation therapy, surgery, hormonal therapy and/or biological therapy/immunotherapy, anti-VEGF antibody therapy, particularly a standard therapeutic regimen for the particular cancer
  • a cancer is relapse tumor growth or relapse cancer cell growth where the number of cancer cells has not been significantly reduced, or has increased, or tumor size has not been significantly reduced, or has increased, or fails any further reduction in size or in number of cancer cells.
  • the determination of whether the cancer cells are relapse tumor growth or relapse cancer cell growth can be made either in vivo or in vitro by any method known in the art for assaying the effectiveness of treatment on cancer cells, using the art-accepted meanings of "relapse” or “refractory” or “non-responsive” in such a context.
  • a tumor resistant to anti-VEGF treatment is an example of a relapse tumor growth.
  • the invention provides methods of blocking or reducing relapse tumor growth or relapse cancer cell growth in a subject by administering one or more VEGF-C antagonist to block or reduce the relapse tumor growth or relapse cancer cell growth in subject.
  • the antagonist can be administered subsequent to the cancer therapeutic.
  • the VEGF-C antagonists are administered simultaneously with cancer therapy.
  • the VEGF-C antagonist therapy alternates with another cancer therapy, which can be performed in any order.
  • the invention also encompasses methods for administering one or more inhibitory antibodies to prevent the onset or recurrence of cancer in patients predisposed to having cancer. Generally, the subject was or is concurrently undergoing cancer therapy.
  • the cancer therapy is treatment with an anti-angiogenesis agent, e.g., a VEGF antagonist.
  • the anti- angiogenesis agent includes those known in the art and those found under the
  • the anti-angiogenesis agent is an anti-VEGF neutralizing antibody or fragment (e.g., humanized A4.6.1, AVASTIN ® (Genentech, South San Francisco, CA), Y0317, M4, G6, B20, 2C3, etc.). See, e.g., U.S.
  • Additional agents can be administered in combination with VEGF antagonist and a VEGF-C antagonist for blocking or reducing relapse tumor growth or relapse cancer cell growth, e.g., see section entitled Combination therapies herein.
  • the invention provides a method of diagnosing a disorder associated with increased expression of VEGF-C.
  • the method comprises contacting a test cell with an anti-VEGF-C antibody;
  • the test cell is obtained from an individual suspected of having a disorder associated with increased expression of VEGF-C.
  • the disorder is a tumor, cancer, and/or cell proliferative disorder.
  • Exemplary disorders that may be diagnosed using an antibody of the invention include, but not limited to, list of disorders, tumors and cancers provided herein under "Definitions.”
  • the invention provides a method of detecting the presence of VEGF-C in a biological sample.
  • the term "detecting” as used herein encompasses quantitative or qualitative detection.
  • the method comprises contacting the biological sample with an anti-VEGF-C antibody under conditions permissive for binding of the anti-VEGF-C antibody to VEGF, and detecting whether a complex is formed between the anti-VEGF-C antibody and VEGF-C.
  • the complex is in vivo or in vitro.
  • the complex comprises a cancer cell.
  • Analytical methods for VEGF-C all use one or more of the following reagents: labeled VEGF-C analogue, immobilized VEGF-C analogue, labeled anti-VEGF-C antibody, immobilized anti-VEGF-C antibody and/or steric conjugates.
  • the labeled reagents also are known as "tracers.”
  • the anti-VEGF-C antibody is detectably labeled.
  • the label used is any detectable functionality that does not interfere with the binding of VEGF-C and anti-VEGF-C antibody.
  • Labels include, but are not limited to, labels or moieties that are detected directly (such as fluorescent, chromophoric, electron-dense, chemilummescent, and radioactive labels), as well as moieties, such as enzymes or ligands, that are detected indirectly, e.g., through an enzymatic reaction or molecular interaction.
  • Exemplary labels include, but are not limited to, the
  • radioisotopes P, C, I, H, and I fluorophores such as rare earth chelates or fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, luceriferases, e.g., firefly luciferase and bacterial luciferase (U.S. Pat. No.
  • luciferin 2,3-dihydrophthalazinediones
  • horseradish peroxidase HRP
  • alkaline phosphatase alkaline phosphatase
  • ⁇ -galactosidase glucoamylase
  • lysozyme saccharide oxidases, e.g., glucose oxidase, galactose oxidase, and glucose-6-phosphate dehydrogenase
  • heterocyclic oxidases such as uricase and xanthine oxidase, coupled with an enzyme that employs hydrogen peroxide to oxidize a dye precursor such as HRP
  • HRP horseradish peroxidase
  • lactoperoxidase or microperoxidase, biotin/avidin, spin labels, bacteriophage labels, stable free radicals, and the like.
  • Preferred labels herein are enzymes such as horseradish peroxidase and alkaline phosphatase.
  • the conjugation of such label, including the enzymes, to the antibody is a standard manipulative procedure for one of ordinary skill in immunoassay techniques. See, for example, O'Sullivan et al., "Methods for the Preparation of Enzyme-antibody
  • antibodies are immobilized on an insoluble matrix. Immobilization may entail separating an anti-VEGF-C antibody from any VEGF-C that remains free in solution. This conventionally is accomplished by either insolubilizing the anti-VEGF-C antibody before the assay procedure, as by adsorption to a water-insoluble matrix or surface (Bennich et al., U.S. 3,720,760), or by covalent coupling (for example, using glutaraldehyde cross-linking), or by insolubilizing the anti-VEGF-C antibody after formation of a complex between the anti-VEGF-C antibody and VEGF-C, e.g., by immunoprecipitation.
  • Anti-VEGF-C antibodies can be used for the detection of VEGF-C in any one of a number of well known detection assay methods.
  • a biological sample may be assayed for VEGF-C by obtaining the sample from a desired source, admixing the sample with anti-VEGF-C antibody to allow the antibody to form antibody/VEGF-C complex with any VEGF-C present in the mixture, and detecting any antibody/VEGF-C complex present in the mixture.
  • the biological sample may be prepared for assay by methods known in the art which are suitable for the particular sample. The methods of admixing the sample with antibodies and the methods of detecting antibody/VEGF-C complex are chosen according to the type of assay used.
  • Assays used to detect binding of anti-VEGF-C antibodies to VEGF-C include, but not limited to, antigen-binding assays that are well known in the art, such as western blots, radioimmunoassays, ELISA (enzyme linked
  • immunosorbent assay competitive and “sandwich” assays, immunoprecipitation assays, fluorescent immunoassays, protein A immunoassays, immunohistochemistry (IHC) and steric inhibition assays.
  • the expression of VEGF-C in a sample may be examined using immunohistochemistry and staining protocols.
  • Immunohistochemical staining of tissue sections has been shown to be a reliable method of assessing or detecting presence of proteins in a sample.
  • Immunohistochemistry techniques utilize an antibody to probe and visualize cellular antigens in situ, generally by chromogenic or fluorescent methods.
  • a tissue or cell sample from a mammal typically a human patient
  • samples include, but are not limited to, cancer cells such as colon, breast, prostate, ovary, lung, stomach, pancreas, lymphoma, and leukemia cancer cells.
  • the sample can be obtained by a variety of procedures known in the art including, but not limited to surgical excision, aspiration or biopsy.
  • the tissue may be fresh or frozen.
  • the sample is fixed and embedded in paraffin or the like.
  • the tissue sample may be fixed (i.e. preserved) by conventional methodology.
  • a fixative is determined by the purpose for which the sample is to be histologically stained or otherwise analyzed.
  • the length of fixation depends upon the size of the tissue sample and the fixative used.
  • IHC may be performed in combination with additional techniques such as morphological staining and/or fluorescence in-situ hybridization.
  • Two general methods of IHC are available; direct and indirect assays.
  • binding of antibody to the target antigen e.g., VEGF-C
  • This direct assay uses a labeled reagent, such as a fluorescent tag or an enzyme-labeled primary antibody, which can be visualized without further antibody interaction.
  • a labeled primary antibody binds to the antigen and then a labeled secondary antibody binds to the primary antibody.
  • a chromogenic or fluorogenic substrate is added to provide visualization of the antigen.
  • Signal amplification occurs because several secondary antibodies may react with different epitopes on the primary antibody.
  • the primary and/or secondary antibody used for immunohistochemistry typically will be labeled with a detectable moiety.
  • tissue section is exposed to primary antibody for a sufficient period of time and under suitable conditions such that the primary antibody binds to the target protein antigen in the tissue sample. Appropriate conditions for achieving this can be determined by routine
  • the label is an enzymatic label (e.g. HRPO) which catalyzes a chemical alteration of the
  • chromogenic substrate such as 3,3 '-diaminobenzidine chromogen.
  • the enzymatic label is conjugated to antibody which binds specifically to the primary antibody (e.g., the primary antibody is rabbit polyclonal antibody and secondary antibody is goat anti-rabbit antibody).
  • Specimens thus prepared may be mounted and coverslipped. Slide evaluation is then determined, e.g., using a microscope, and staining intensity criteria, routinely used in the art, may be employed. Staining intensity criteria may be evaluated as follows:
  • a staining pattern score of about 2+ or higher in an IHC assay is diagnostic and/or prognostic. In some embodiments, a staining pattern score of about 1+ or higher is diagnostic and/or prognostic. In other embodiments, a staining pattern score of about 3 of higher is diagnostic and/or prognostic. It is understood that when cells and/or tissue from a tumor or colon adenoma are examined using IHC, staining is generally determined or assessed in tumor cell and/or tissue (as opposed to stromal or surrounding tissue that may be present in the sample). [0513] Other assay methods, known as competitive or sandwich assays, are well established and widely used in the commercial diagnostics industry.
  • a routine cross-blocking assay such as that described in Antibodies, A Laboratory Manual, Cold Spring Harbor Laboratory, Ed Harlow and David Lane (1988), can be performed.
  • epitope mapping e.g. as described in Champe et al. (1995) J. Biol. Chem. 270: 1388-1394, can be performed to determine whether the antibody binds an epitope of interest.
  • Detailed exemplary methods for mapping an epitope to which an antibody binds are also provided in Morris (1996) "Epitope Mapping Protocols," in Methods in Molecular Biology vol. 66 (Humana Press, Totowa, NJ). Two antibodies are said to bind to the same epitope if each blocks binding of the other by 50% or more.
  • test sample VEGF-C is inversely proportional to the amount of bound tracer as measured by the amount of marker substance.
  • Dose-response curves with known amounts of VEGF-C are prepared and compared with the test results to quantitatively determine the amount of VEGF-C present in the test sample. These assays are called ELISA systems when enzymes are used as the detectable markers.
  • Another species of competitive assay does not require a phase separation.
  • a conjugate of an enzyme with the VEGF-C is prepared and used such that when anti-VEGF-C antibody binds to the VEGF-C the presence of the anti-VEGF-C antibody modifies the enzyme activity.
  • the VEGF-C or its immunologically active fragments are conjugated with a bifunctional organic bridge to an enzyme such as peroxidase.
  • Conjugates are selected for use with anti-VEGF-C antibody so that binding of the anti-VEGF-C antibody inhibits or potentiates the enzyme activity of the label. This method per se is widely practiced under the name of EMIT.
  • Steric conjugates are used in steric hindrance methods for homogeneous assay. These conjugates are synthesized by covalently linking a low- molecular-weight hapten to a small VEGF-C fragment so that antibody to hapten is substantially unable to bind the conjugate at the same time as anti-VEGF-C antibody. Under this assay procedure the VEGF-C present in the test sample will bind anti- VEGF-C antibody, thereby allowing anti -hapten to bind the conjugate, resulting in a change in the character of the conjugate hapten, e.g., a change in fluorescence when the hapten is a fluorophore.
  • Sandwich assays particularly are useful for the determination of VEGF-C or anti-VEGF-C antibodies.
  • an immobilized anti-VEGF-C antibody is used to adsorb test sample VEGF-C, the test sample is removed as by washing, the bound VEGF-C is used to adsorb a second, labeled anti- VEGF-C antibody and bound material is then separated from residual tracer. The amount of bound tracer is directly proportional to test sample VEGF-C.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Endocrinology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne des antagonistes du VEGF-C, tels que des anticorps anti-VEGF-C, et leur utilisation dans la prévention et le traitement de l'évolution d'une tumeur.
PCT/US2010/048908 2009-12-11 2010-09-15 Anticorps anti-vegf-c et leurs procédés d'utilisation WO2011071577A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES10757138.2T ES2565208T3 (es) 2009-12-11 2010-09-15 Anticuerpos anti-VEGF-C y métodos de uso de los mismos
EP10757138.2A EP2509626B1 (fr) 2009-12-11 2010-09-15 Anticorps anti-vegf-c et leurs procédés d'utilisation
JP2012543094A JP5818805B2 (ja) 2009-12-11 2010-09-15 抗ｖｅｇｆ−ｃ抗体及びその使用方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US28591009P 2009-12-11 2009-12-11
US61/285,910 2009-12-11
US28475309P 2009-12-23 2009-12-23
US61/284,753 2009-12-23

Publications (1)

Publication Number Publication Date
WO2011071577A1 true WO2011071577A1 (fr) 2011-06-16

Family

ID=43040734

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/048908 WO2011071577A1 (fr) 2009-12-11 2010-09-15 Anticorps anti-vegf-c et leurs procédés d'utilisation

Country Status (7)

Country Link
US (2) US8486397B2 (fr)
EP (1) EP2509626B1 (fr)
JP (1) JP5818805B2 (fr)
AR (1) AR078377A1 (fr)
ES (1) ES2565208T3 (fr)
TW (1) TWI505836B (fr)
WO (1) WO2011071577A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014064203A1 (fr) 2012-10-26 2014-05-01 INSERM (Institut National de la Santé et de la Recherche Médicale) Antagonistes lyve-1 utiles dans la prévention ou le traitement d'un état pathologique associé à la lymphangiogenèse
EP3272771A1 (fr) 2016-07-22 2018-01-24 Centre National De La Recherche Scientifique Traitement destiné à être utilisé pour prévenir les métastases chez un sujet exposé à un traitement anticancéreux induisant l'activation p38
WO2018189403A1 (fr) 2017-04-14 2018-10-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Méthodes et compositions pharmaceutiques destinées au traitement du cancer

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011075185A1 (fr) 2009-12-18 2011-06-23 Oligasis Conjugués de polymère de phosphorylcholine à médicament ciblé
WO2015035342A2 (fr) 2013-09-08 2015-03-12 Oligasis Llc Conjugués polymère zwittérionique-facteur viii
KR102131370B1 (ko) * 2013-10-18 2020-07-08 삼성전자주식회사 Vegf-c에 특이적으로 결합하는 폴리펩타이드 및 그의 용도
US9840553B2 (en) 2014-06-28 2017-12-12 Kodiak Sciences Inc. Dual PDGF/VEGF antagonists
CN107208076A (zh) 2014-10-17 2017-09-26 科达制药 丁酰胆碱酯酶两性离子聚合物缀合物
US11066465B2 (en) 2015-12-30 2021-07-20 Kodiak Sciences Inc. Antibodies and conjugates thereof
CN112203679A (zh) 2018-03-02 2021-01-08 科达制药股份有限公司 Il-6抗体及其融合构建体和缀合物
CA3157509A1 (fr) 2019-10-10 2021-04-15 Kodiak Sciences Inc. Procedes de traitement d'un trouble oculaire
EP4386004A1 (fr) * 2021-08-13 2024-06-19 Innovent Biologics (Suzhou) Co., Ltd. Anticorps bispécifique anti-vegf a et anti-vegf c et son utilisation
KR20230109854A (ko) * 2022-01-14 2023-07-21 주식회사 파멥신 항-vegfr2(kdr) 개량 항체를 포함하는 융합단백질 및 이의 용도

Citations (184)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3645090A (en) 1969-06-19 1972-02-29 Citizen Watch Co Ltd Day-date quick-adjuster for calender timepiece
US3720760A (en) 1968-09-06 1973-03-13 Pharmacia Ab Method for determining the presence of reagin-immunoglobulins(reagin-ig)directed against certain allergens,in aqueous samples
US3773919A (en) 1969-10-23 1973-11-20 Du Pont Polylactide-drug mixtures
US3896111A (en) 1973-02-20 1975-07-22 Research Corp Ansa macrolides
US3940475A (en) 1970-06-11 1976-02-24 Biological Developments, Inc. Radioimmune method of assaying quantitatively for a hapten
US4137230A (en) 1977-11-14 1979-01-30 Takeda Chemical Industries, Ltd. Method for the production of maytansinoids
US4151042A (en) 1977-03-31 1979-04-24 Takeda Chemical Industries, Ltd. Method for producing maytansinol and its derivatives
EP0003089A1 (fr) 1978-01-06 1979-07-25 Bernard David Séchoir pour feuilles imprimées par sérigraphie
US4248870A (en) 1978-10-27 1981-02-03 Takeda Chemical Industries, Ltd. Maytansinoids and use
US4256746A (en) 1978-11-14 1981-03-17 Takeda Chemical Industries Dechloromaytansinoids, their pharmaceutical compositions and method of use
US4260608A (en) 1978-11-14 1981-04-07 Takeda Chemical Industries, Ltd. Maytansinoids, pharmaceutical compositions thereof and methods of use thereof
US4265814A (en) 1978-03-24 1981-05-05 Takeda Chemical Industries Matansinol 3-n-hexadecanoate
US4294757A (en) 1979-01-31 1981-10-13 Takeda Chemical Industries, Ltd 20-O-Acylmaytansinoids
US4307016A (en) 1978-03-24 1981-12-22 Takeda Chemical Industries, Ltd. Demethyl maytansinoids
US4308269A (en) 1979-06-11 1981-12-29 Takeda Chemical Industries, Ltd. Maytansinoids, pharmaceutical compositions thereof and method of use thereof
US4308268A (en) 1979-06-11 1981-12-29 Takeda Chemical Industries, Ltd. Maytansinoids, pharmaceutical compositions thereof and method of use thereof
US4309428A (en) 1979-07-30 1982-01-05 Takeda Chemical Industries, Ltd. Maytansinoids
US4313946A (en) 1981-01-27 1982-02-02 The United States Of America As Represented By The Secretary Of Agriculture Chemotherapeutically active maytansinoids from Trewia nudiflora
US4315929A (en) 1981-01-27 1982-02-16 The United States Of America As Represented By The Secretary Of Agriculture Method of controlling the European corn borer with trewiasine
US4317821A (en) 1979-06-08 1982-03-02 Takeda Chemical Industries, Ltd. Maytansinoids, their use and pharmaceutical compositions thereof
US4322348A (en) 1979-06-05 1982-03-30 Takeda Chemical Industries, Ltd. Maytansinoids
US4331598A (en) 1979-09-19 1982-05-25 Takeda Chemical Industries, Ltd. Maytansinoids
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
US4362663A (en) 1979-09-21 1982-12-07 Takeda Chemical Industries, Ltd. Maytansinoid compound
US4364866A (en) 1979-09-21 1982-12-21 Takeda Chemical Industries, Ltd. Maytansinoids
US4371533A (en) 1980-10-08 1983-02-01 Takeda Chemical Industries, Ltd. 4,5-Deoxymaytansinoids, their use and pharmaceutical compositions thereof
EP0073657A1 (fr) 1981-08-31 1983-03-09 Genentech, Inc. Préparation d'antigène de surface de l'hépatite B dans une levure
US4419446A (en) 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
US4424219A (en) 1981-05-20 1984-01-03 Takeda Chemical Industries, Ltd. 9-Thiomaytansinoids and their pharmaceutical compositions and use
US4450254A (en) 1980-11-03 1984-05-22 Standard Oil Company Impact improvement of high nitrile resins
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
EP0183070A2 (fr) 1984-10-30 1986-06-04 Phillips Petroleum Company Transformation de levures du genre Pichia
US4601978A (en) 1982-11-24 1986-07-22 The Regents Of The University Of California Mammalian metallothionein promoter system
WO1987000195A1 (fr) 1985-06-28 1987-01-15 Celltech Limited Culture de cellules animales
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
EP0244234A2 (fr) 1986-04-30 1987-11-04 Alko Group Ltd. Transformation de trichoderma
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
WO1990003430A1 (fr) 1988-09-23 1990-04-05 Cetus Corporation Milieu de culture de cellules pour l'amelioration de la croissance des cellules, de la longivite de la culture et de l'expression du produit
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
US4965199A (en) 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
US4970198A (en) 1985-10-17 1990-11-13 American Cyanamid Company Antitumor antibiotics (LL-E33288 complex)
WO1990013646A1 (fr) 1989-04-28 1990-11-15 Transgene S.A. Application de nouveaux fragments d'adn en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
US4975278A (en) 1988-02-26 1990-12-04 Bristol-Myers Company Antibody-enzyme conjugates in combination with prodrugs for the delivery of cytotoxic agents to tumor cells
EP0402226A1 (fr) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Vecteurs de transformation de la levure yarrowia
EP0404097A2 (fr) 1989-06-22 1990-12-27 BEHRINGWERKE Aktiengesellschaft Récepteurs mono- et oligovalents, bispécifiques et oligospécifiques, ainsi que leur production et application
WO1991000360A1 (fr) 1989-06-29 1991-01-10 Medarex, Inc. Reactifs bispecifiques pour le traitement du sida
US5004697A (en) 1987-08-17 1991-04-02 Univ. Of Ca Cationized antibodies for delivery through the blood-brain barrier
WO1991010741A1 (fr) 1990-01-12 1991-07-25 Cell Genesys, Inc. Generation d'anticorps xenogeniques
US5053394A (en) 1988-09-21 1991-10-01 American Cyanamid Company Targeted forms of methyltrithio antitumor agents
US5079233A (en) 1987-01-30 1992-01-07 American Cyanamid Company N-acyl derivatives of the LL-E33288 antitumor antibiotics, composition and methods for using the same
WO1992000373A1 (fr) 1990-06-29 1992-01-09 Biosource Genetics Corporation Production de melanines a l'aide de microorganismes transformes
US5112596A (en) 1990-04-23 1992-05-12 Alkermes, Inc. Method for increasing blood-brain barrier permeability by administering a bradykinin agonist of blood-brain barrier permeability
WO1992009690A2 (fr) 1990-12-03 1992-06-11 Genentech, Inc. Methode d'enrichissement pour des variantes de l'hormone de croissance avec des proprietes de liaison modifiees
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
WO1993001161A1 (fr) 1991-07-11 1993-01-21 Pfizer Limited Procede de preparation d'intermediaires de sertraline
WO1993006213A1 (fr) 1991-09-23 1993-04-01 Medical Research Council Production d'anticorps chimeriques - une approche combinatoire
US5208020A (en) 1989-10-25 1993-05-04 Immunogen Inc. Cytotoxic agents comprising maytansinoids and their therapeutic use
WO1993008829A1 (fr) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions induisant la destruction de cellules infectees par l'hiv
WO1993016185A2 (fr) 1992-02-06 1993-08-19 Creative Biomolecules, Inc. Proteine de liaison biosynthetique pour marqueur de cancer
WO1993021232A1 (fr) 1992-04-10 1993-10-28 Research Development Foundation IMMUNOTOXINES DIRIGEES CONTRE DES ANTIGENES DE SURFACE APPARENTEES A c-erbB-2(HER-2/neu)
US5268164A (en) 1990-04-23 1993-12-07 Alkermes, Inc. Increasing blood-brain barrier permeability with permeabilizer peptides
WO1994004690A1 (fr) 1992-08-17 1994-03-03 Genentech, Inc. Immunoadhesines bispecifiques
WO1994010202A1 (fr) 1992-10-28 1994-05-11 Genentech, Inc. Antagonistes du facteur de croissance des cellules endotheliales vasculaires
WO1994011026A2 (fr) 1992-11-13 1994-05-26 Idec Pharmaceuticals Corporation Application therapeutique d'anticorps chimeriques et radio-marques contre l'antigene a differentiation restreinte des lymphocytes b humains pour le traitement du lymphome des cellules b
US5362852A (en) 1991-09-27 1994-11-08 Pfizer Inc. Modified peptide derivatives conjugated at 2-hydroxyethylamine moieties
WO1994029351A2 (fr) 1993-06-16 1994-12-22 Celltech Limited Anticorps
WO1996007754A1 (fr) 1994-09-02 1996-03-14 The Scripps Research Institute Procedes de production de banques d'anticorps au moyen de chaines legeres d'immunoglobuline universelles ou rendues alleatoires
US5500362A (en) 1987-01-08 1996-03-19 Xoma Corporation Chimeric antibody with specificity to human B cell surface antigen
US5545807A (en) 1988-10-12 1996-08-13 The Babraham Institute Production of antibodies from transgenic animals
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
WO1996027011A1 (fr) 1995-03-01 1996-09-06 Genentech, Inc. Procede d'obtention de polypeptides heteromultimeriques
EP0425235B1 (fr) 1989-10-25 1996-09-25 Immunogen Inc Agents cytotoxiques contenant des maytansinoides et leur application thérapeutique
WO1996030046A1 (fr) 1995-03-30 1996-10-03 Genentech, Inc. Antagonistes de facteurs de croissance des cellules endotheliales vasculaires
US5569825A (en) 1990-08-29 1996-10-29 Genpharm International Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
WO1996034096A1 (fr) 1995-04-28 1996-10-31 Abgenix, Inc. Anticorps humains derives de xeno-souris immunisees
WO1996033735A1 (fr) 1995-04-27 1996-10-31 Abgenix, Inc. Anticorps humains derives d'une xenosouris immunisee
US5571894A (en) 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
US5585089A (en) 1988-12-28 1996-12-17 Protein Design Labs, Inc. Humanized immunoglobulins
US5587458A (en) 1991-10-07 1996-12-24 Aronex Pharmaceuticals, Inc. Anti-erbB-2 antibodies, combinations thereof, and therapeutic and diagnostic uses thereof
US5606040A (en) 1987-10-30 1997-02-25 American Cyanamid Company Antitumor and antibacterial substituted disulfide derivatives prepared from compounds possessing a methyl-trithio group
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5624821A (en) 1987-03-18 1997-04-29 Scotgen Biopharmaceuticals Incorporated Antibodies with altered effector functions
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5635483A (en) 1992-12-03 1997-06-03 Arizona Board Of Regents Acting On Behalf Of Arizona State University Tumor inhibiting tetrapeptide bearing modified phenethyl amides
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5648237A (en) 1991-09-19 1997-07-15 Genentech, Inc. Expression of functional antibody fragments
WO1997030087A1 (fr) 1996-02-16 1997-08-21 Glaxo Group Limited Preparation d'anticorps glycosyles
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5663149A (en) 1994-12-13 1997-09-02 Arizona Board Of Regents Acting On Behalf Of Arizona State University Human cancer inhibitory pentapeptide heterocyclic and halophenyl amides
US5712374A (en) 1995-06-07 1998-01-27 American Cyanamid Company Method for the preparation of substantiallly monomeric calicheamicin derivative/carrier conjugates
US5714586A (en) 1995-06-07 1998-02-03 American Cyanamid Company Methods for the preparation of monomeric calicheamicin derivative/carrier conjugates
US5739116A (en) 1994-06-03 1998-04-14 American Cyanamid Company Enediyne derivatives useful for the synthesis of conjugates of methyltrithio antitumor agents
WO1998024893A2 (fr) 1996-12-03 1998-06-11 Abgenix, Inc. MAMMIFERES TRANSGENIQUES POSSEDANT DES LOCI DE GENES D'IMMUNOGLOBULINE D'ORIGINE HUMAINE, DOTES DE REGIONS VH ET Vλ, ET ANTICORPS PRODUITS A PARTIR DE TELS MAMMIFERES
US5770701A (en) 1987-10-30 1998-06-23 American Cyanamid Company Process for preparing targeted forms of methyltrithio antitumor agents
US5780588A (en) 1993-01-26 1998-07-14 Arizona Board Of Regents Elucidation and synthesis of selected pentapeptides
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5821337A (en) 1991-06-14 1998-10-13 Genentech, Inc. Immunoglobulin variants
WO1998045332A2 (fr) 1997-04-07 1998-10-15 Genentech, Inc. Anticorps humanises et methode permettant de les produire
WO1998045331A2 (fr) 1997-04-07 1998-10-15 Genentech, Inc. Anticorps anti-vegf
WO1998050431A2 (fr) 1997-05-02 1998-11-12 Genentech, Inc. Procede de preparation d'anticorps multispecifiques presentant des composants heteromultimeres
US5840523A (en) 1995-03-01 1998-11-24 Genetech, Inc. Methods and compositions for secretion of heterologous polypeptides
WO1998058964A1 (fr) 1997-06-24 1998-12-30 Genentech, Inc. Procedes et compositions concernant des glycoproteines galactosylees
US5869046A (en) 1995-04-14 1999-02-09 Genentech, Inc. Altered polypeptides with increased half-life
WO1999022764A1 (fr) 1997-10-31 1999-05-14 Genentech, Inc. Compositions renfermant des glycoformes de glycoproteine et methodes afferentes
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
WO1999051642A1 (fr) 1998-04-02 1999-10-14 Genentech, Inc. Variants d'anticorps et fragments de ceux-ci
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
US6054297A (en) 1991-06-14 2000-04-25 Genentech, Inc. Humanized antibodies and methods for making them
WO2000029004A1 (fr) 1998-11-18 2000-05-25 Peptor Ltd. Petites unites fonctionnelles de regions variables a chaine lourde d'anticorps
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
WO2000042072A2 (fr) 1999-01-15 2000-07-20 Genentech, Inc. Variants polypeptidiques ayant une fonction effectrice alteree
WO2000061739A1 (fr) 1999-04-09 2000-10-19 Kyowa Hakko Kogyo Co., Ltd. Methode de regulation de l'activite d'une molecule immunologiquement fonctionnelle
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
WO2001029246A1 (fr) 1999-10-19 2001-04-26 Kyowa Hakko Kogyo Co., Ltd. Procede de production d'un polypeptide
US6248516B1 (en) 1988-11-11 2001-06-19 Medical Research Council Single domain ligands, receptors comprising said ligands methods for their production, and use of said ligands and receptors
US20020025313A1 (en) 1992-07-27 2002-02-28 Micklus Michael J. Targeting of liposomes to the blood-brain barrier
US20020038086A1 (en) 2000-07-27 2002-03-28 Hynynen Kullervo H. Blood-brain barrier opening
WO2002031140A1 (fr) 2000-10-06 2002-04-18 Kyowa Hakko Kogyo Co., Ltd. Cellules produisant des compositions d'anticorps
US20020065259A1 (en) 2000-08-30 2002-05-30 Schatzberg Alan F. Glucocorticoid blocking agents for increasing blood-brain barrier permeability
WO2002051870A2 (fr) 2000-12-22 2002-07-04 GRAD, Carole Legal Representative of KAPLAN, Howard Bibliotheques d'affichage de phages de fragments vh humains
US6420548B1 (en) 1999-10-04 2002-07-16 Medicago Inc. Method for regulating transcription of foreign genes
US20020164328A1 (en) 2000-10-06 2002-11-07 Toyohide Shinkawa Process for purifying antibody
WO2002088172A2 (fr) 2001-04-30 2002-11-07 Seattle Genetics, Inc. Composes pentapeptidiques et leurs utilisations
WO2003011878A2 (fr) 2001-08-03 2003-02-13 Glycart Biotechnology Ag Variants de glycosylation d'anticorps presentant une cytotoxicite cellulaire accrue dependante des anticorps
US20030055006A1 (en) 2000-06-23 2003-03-20 Schering Aktiengesellschaft Combinations and compositions which interfere with VEGF/VEGF and angiopoietin/tie receptor function and their use
US20030073713A1 (en) 2000-10-30 2003-04-17 Schoenhard Grant L. Inhibitors of ABC drug transporters at the blood-brain barrier
US20030083299A1 (en) 2000-11-04 2003-05-01 Ferguson Ian A. Non-invasive delivery of polypeptides through the blood-brain barrier
WO2003035694A2 (fr) 2001-10-24 2003-05-01 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Anticorps fonctionnels a chaine lourde, fragments de ces derniers, bibliotheque de ces derniers et procedes de production
US20030115614A1 (en) 2000-10-06 2003-06-19 Yutaka Kanda Antibody composition-producing cell
US6582959B2 (en) 1991-03-29 2003-06-24 Genentech, Inc. Antibodies to vascular endothelial cell growth factor
US20030129186A1 (en) 2001-07-25 2003-07-10 Biomarin Pharmaceutical Inc. Compositions and methods for modulating blood-brain barrier transport
US6602684B1 (en) 1998-04-20 2003-08-05 Glycart Biotechnology Ag Glycosylation engineering of antibodies for improving antibody-dependent cellular cytotoxicity
US20030157108A1 (en) 2001-10-25 2003-08-21 Genentech, Inc. Glycoprotein compositions
US20030162695A1 (en) 2002-02-27 2003-08-28 Schatzberg Alan F. Glucocorticoid blocking agents for increasing blood-brain barrier permeability
US20030190317A1 (en) 1997-04-07 2003-10-09 Genentech, Inc. Anti-VEGF antibodies
WO2003085107A1 (fr) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Cellules à génome modifié
WO2003085119A1 (fr) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Procede d'amelioration de l'activite d'une composition d'anticorps de liaison avec le recepteur fc$g(g) iiia
WO2003084570A1 (fr) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Medicament contenant une composition d'anticorps appropriee au patient souffrant de polymorphisme fc$g(g)riiia
US20030206899A1 (en) 1991-03-29 2003-11-06 Genentech, Inc. Vascular endothelial cell growth factor antagonists
EP1391213A1 (fr) 2002-08-21 2004-02-25 Boehringer Ingelheim International GmbH Compositions et méthodes pour le traitement du cancer en utilisant un conjugué d'un anticorps contre le CD44 avec un maytansinoide et des agents chimiothérapeutiques
US6703020B1 (en) 1999-04-28 2004-03-09 Board Of Regents, The University Of Texas System Antibody conjugate methods for selectively inhibiting VEGF
US20040093621A1 (en) 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
US6737056B1 (en) 1999-01-15 2004-05-18 Genentech, Inc. Polypeptide variants with altered effector function
US20040110282A1 (en) 2002-04-09 2004-06-10 Kyowa Hakko Kogyo Co., Ltd. Cells in which activity of the protein involved in transportation of GDP-fucose is reduced or lost
US20040109865A1 (en) 2002-04-09 2004-06-10 Kyowa Hakko Kogyo Co., Ltd. Antibody composition-containing medicament
US20040132140A1 (en) 2002-04-09 2004-07-08 Kyowa Hakko Kogyo Co., Ltd. Production process for antibody composition
US20040131692A1 (en) 2001-05-05 2004-07-08 Joerg Kreuter Nanoparticles made of protein with coupled apolipoprotein e for penetration of the blood-brain barrier and methods for the production thereof
WO2004056312A2 (fr) 2002-12-16 2004-07-08 Genentech, Inc. Variants d'immunoglobuline et utilisations
US20040147448A1 (en) * 1995-08-01 2004-07-29 Kari Alitalo Vascular endothelial growth factor C (VEGF-C) protein and gene, mutants thereof, and uses thereof
US20040204354A1 (en) 2002-12-03 2004-10-14 Thomas Nelson Artificial low-density lipoprotein carriers for transport of substances across the blood-brain barrier
WO2004092219A2 (fr) 2003-04-10 2004-10-28 Protein Design Labs, Inc Modification d'affinites de liaison pour fcrn ou de demi-vies de serum d'anticorps par mutagenese
WO2004113304A1 (fr) 2003-05-22 2004-12-29 Abbott Laboratories Inhibiteurs de kinases de type indazole, benzisoxazole et benzisothiazole
US20050014934A1 (en) 2002-10-15 2005-01-20 Hinton Paul R. Alteration of FcRn binding affinities or serum half-lives of antibodies by mutagenesis
US20050026229A1 (en) 1997-03-10 2005-02-03 The Regents Of The University Of California PSCA antibodies and hybridomas producing them
WO2005012359A2 (fr) 2003-08-01 2005-02-10 Genentech, Inc. Anticorps anti-vegf
WO2005035572A2 (fr) 2003-10-08 2005-04-21 Domantis Limited Compositions d'anticorps et procedes
WO2005035778A1 (fr) 2003-10-09 2005-04-21 Kyowa Hakko Kogyo Co., Ltd. Procede permettant de produire une composition d'anticorps par inhibition par l'arn de la fonction de $g(a)1,6-fucosyltransferase
WO2005035586A1 (fr) 2003-10-08 2005-04-21 Kyowa Hakko Kogyo Co., Ltd. Composition proteique hybride
US6884879B1 (en) 1997-04-07 2005-04-26 Genentech, Inc. Anti-VEGF antibodies
US20050089473A1 (en) 2003-09-10 2005-04-28 Cedars-Sinai Medical Center Potassium channel mediated delivery of agents through the blood-brain barrier
US20050100546A1 (en) 1997-05-05 2005-05-12 Abgenix, Inc. Human monoclonal antibodies to epidermal growth factor receptor
US20050123546A1 (en) 2003-11-05 2005-06-09 Glycart Biotechnology Ag Antigen binding molecules with increased Fc receptor binding affinity and effector function
WO2005053742A1 (fr) 2003-12-04 2005-06-16 Kyowa Hakko Kogyo Co., Ltd. Medicament contenant une composition a base d'anticorps
US6919436B2 (en) 1996-08-30 2005-07-19 Upfront Chromatography A/S Isolation of proteins
US20050186208A1 (en) 2003-05-30 2005-08-25 Genentech, Inc. Treatment with anti-VEGF antibodies
US20050287149A1 (en) 2003-05-21 2005-12-29 Medarex, Inc. Human monoclonal antibodies against bacillus anthracis protective antigen
US6982321B2 (en) 1986-03-27 2006-01-03 Medical Research Council Altered antibodies
US20060009360A1 (en) 2004-06-25 2006-01-12 Robert Pifer New adjuvant composition
US20060059575A1 (en) 1999-03-30 2006-03-16 Japan Tobacco, Inc. Method for preparing monoclonal antibody
US7078492B2 (en) 2001-05-16 2006-07-18 Abgenix, Inc. Human antipneumococcal antibodies from non-human animals
US7087409B2 (en) 1997-12-05 2006-08-08 The Scripps Research Institute Humanization of murine antibody
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
US20060258841A1 (en) 2003-01-17 2006-11-16 Josef Michl Pancreatic cancer associated antigen, antibody thereto, and diagnostic and treatment methods
US7153507B2 (en) 2001-08-23 2006-12-26 Genmab A/S Human antibodies specific for interleukin 15 (IL-15)
US20070020267A1 (en) 2003-08-01 2007-01-25 Genentech, Inc. Anti-VEGF antibodies
US7189826B2 (en) 1997-11-24 2007-03-13 Institute For Human Genetics And Biochemistry Monoclonal human natural antibodies
WO2007056470A2 (fr) 2005-11-08 2007-05-18 Genentech, Inc. Antagonistes de la neuropiline
US20080181888A1 (en) 2004-12-31 2008-07-31 Ambrose Christine M Polypeptides That Bind Br3 and Uses Thereof
US20090142343A1 (en) 2007-11-30 2009-06-04 Genentech, Inc. Anti-VEGF antibodies

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451764B1 (en) 1995-09-08 2002-09-17 Genentech, Inc. VEGF-related protein
ATE359300T1 (de) * 2001-01-19 2007-05-15 Ludwig Inst Cancer Res Flt4 (vegfr-3) als ein ziel für krebs darstellung und anti-krebs-behandlung
US20100029491A1 (en) 2008-07-11 2010-02-04 Maike Schmidt Methods and compositions for diagnostic use for tumor treatment
MX2012002862A (es) 2009-09-11 2012-08-15 Genentech Inc Metodo para identificar un paciente con una mayor probabilidad de responder a un agente anticancerigeno.

Patent Citations (210)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3720760A (en) 1968-09-06 1973-03-13 Pharmacia Ab Method for determining the presence of reagin-immunoglobulins(reagin-ig)directed against certain allergens,in aqueous samples
US3720760B1 (fr) 1968-09-06 1984-02-07 Pharmacia Ab
US3645090A (en) 1969-06-19 1972-02-29 Citizen Watch Co Ltd Day-date quick-adjuster for calender timepiece
US3773919A (en) 1969-10-23 1973-11-20 Du Pont Polylactide-drug mixtures
US3940475A (en) 1970-06-11 1976-02-24 Biological Developments, Inc. Radioimmune method of assaying quantitatively for a hapten
US3896111A (en) 1973-02-20 1975-07-22 Research Corp Ansa macrolides
US4151042A (en) 1977-03-31 1979-04-24 Takeda Chemical Industries, Ltd. Method for producing maytansinol and its derivatives
US4137230A (en) 1977-11-14 1979-01-30 Takeda Chemical Industries, Ltd. Method for the production of maytansinoids
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
EP0003089A1 (fr) 1978-01-06 1979-07-25 Bernard David Séchoir pour feuilles imprimées par sérigraphie
US4265814A (en) 1978-03-24 1981-05-05 Takeda Chemical Industries Matansinol 3-n-hexadecanoate
US4361650A (en) 1978-03-24 1982-11-30 Takeda Chemical Industries, Ltd. Fermentation process of preparing demethyl maytansinoids
US4307016A (en) 1978-03-24 1981-12-22 Takeda Chemical Industries, Ltd. Demethyl maytansinoids
US4248870A (en) 1978-10-27 1981-02-03 Takeda Chemical Industries, Ltd. Maytansinoids and use
US4260608A (en) 1978-11-14 1981-04-07 Takeda Chemical Industries, Ltd. Maytansinoids, pharmaceutical compositions thereof and methods of use thereof
US4256746A (en) 1978-11-14 1981-03-17 Takeda Chemical Industries Dechloromaytansinoids, their pharmaceutical compositions and method of use
US4294757A (en) 1979-01-31 1981-10-13 Takeda Chemical Industries, Ltd 20-O-Acylmaytansinoids
US4322348A (en) 1979-06-05 1982-03-30 Takeda Chemical Industries, Ltd. Maytansinoids
US4317821A (en) 1979-06-08 1982-03-02 Takeda Chemical Industries, Ltd. Maytansinoids, their use and pharmaceutical compositions thereof
US4308269A (en) 1979-06-11 1981-12-29 Takeda Chemical Industries, Ltd. Maytansinoids, pharmaceutical compositions thereof and method of use thereof
US4308268A (en) 1979-06-11 1981-12-29 Takeda Chemical Industries, Ltd. Maytansinoids, pharmaceutical compositions thereof and method of use thereof
US4309428A (en) 1979-07-30 1982-01-05 Takeda Chemical Industries, Ltd. Maytansinoids
US4331598A (en) 1979-09-19 1982-05-25 Takeda Chemical Industries, Ltd. Maytansinoids
US4362663A (en) 1979-09-21 1982-12-07 Takeda Chemical Industries, Ltd. Maytansinoid compound
US4364866A (en) 1979-09-21 1982-12-21 Takeda Chemical Industries, Ltd. Maytansinoids
US4371533A (en) 1980-10-08 1983-02-01 Takeda Chemical Industries, Ltd. 4,5-Deoxymaytansinoids, their use and pharmaceutical compositions thereof
US4450254A (en) 1980-11-03 1984-05-22 Standard Oil Company Impact improvement of high nitrile resins
US4419446A (en) 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
US4315929A (en) 1981-01-27 1982-02-16 The United States Of America As Represented By The Secretary Of Agriculture Method of controlling the European corn borer with trewiasine
US4313946A (en) 1981-01-27 1982-02-02 The United States Of America As Represented By The Secretary Of Agriculture Chemotherapeutically active maytansinoids from Trewia nudiflora
US4424219A (en) 1981-05-20 1984-01-03 Takeda Chemical Industries, Ltd. 9-Thiomaytansinoids and their pharmaceutical compositions and use
EP0073657A1 (fr) 1981-08-31 1983-03-09 Genentech, Inc. Préparation d'antigène de surface de l'hépatite B dans une levure
US4601978A (en) 1982-11-24 1986-07-22 The Regents Of The University Of California Mammalian metallothionein promoter system
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4965199A (en) 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
EP0183070A2 (fr) 1984-10-30 1986-06-04 Phillips Petroleum Company Transformation de levures du genre Pichia
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
WO1987000195A1 (fr) 1985-06-28 1987-01-15 Celltech Limited Culture de cellules animales
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
US4970198A (en) 1985-10-17 1990-11-13 American Cyanamid Company Antitumor antibiotics (LL-E33288 complex)
US6982321B2 (en) 1986-03-27 2006-01-03 Medical Research Council Altered antibodies
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
EP0244234A2 (fr) 1986-04-30 1987-11-04 Alko Group Ltd. Transformation de trichoderma
US5500362A (en) 1987-01-08 1996-03-19 Xoma Corporation Chimeric antibody with specificity to human B cell surface antigen
US5079233A (en) 1987-01-30 1992-01-07 American Cyanamid Company N-acyl derivatives of the LL-E33288 antitumor antibiotics, composition and methods for using the same
US5648260A (en) 1987-03-18 1997-07-15 Scotgen Biopharmaceuticals Incorporated DNA encoding antibodies with altered effector functions
US5624821A (en) 1987-03-18 1997-04-29 Scotgen Biopharmaceuticals Incorporated Antibodies with altered effector functions
US5004697A (en) 1987-08-17 1991-04-02 Univ. Of Ca Cationized antibodies for delivery through the blood-brain barrier
US5770701A (en) 1987-10-30 1998-06-23 American Cyanamid Company Process for preparing targeted forms of methyltrithio antitumor agents
US5770710A (en) 1987-10-30 1998-06-23 American Cyanamid Company Antitumor and antibacterial substituted disulfide derivatives prepared from compounds possessing a methlytrithio group
US5606040A (en) 1987-10-30 1997-02-25 American Cyanamid Company Antitumor and antibacterial substituted disulfide derivatives prepared from compounds possessing a methyl-trithio group
US4975278A (en) 1988-02-26 1990-12-04 Bristol-Myers Company Antibody-enzyme conjugates in combination with prodrugs for the delivery of cytotoxic agents to tumor cells
US5053394A (en) 1988-09-21 1991-10-01 American Cyanamid Company Targeted forms of methyltrithio antitumor agents
WO1990003430A1 (fr) 1988-09-23 1990-04-05 Cetus Corporation Milieu de culture de cellules pour l'amelioration de la croissance des cellules, de la longivite de la culture et de l'expression du produit
US5545807A (en) 1988-10-12 1996-08-13 The Babraham Institute Production of antibodies from transgenic animals
US6248516B1 (en) 1988-11-11 2001-06-19 Medical Research Council Single domain ligands, receptors comprising said ligands methods for their production, and use of said ligands and receptors
US5585089A (en) 1988-12-28 1996-12-17 Protein Design Labs, Inc. Humanized immunoglobulins
US5693762A (en) 1988-12-28 1997-12-02 Protein Design Labs, Inc. Humanized immunoglobulins
WO1990013646A1 (fr) 1989-04-28 1990-11-15 Transgene S.A. Application de nouveaux fragments d'adn en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
EP0402226A1 (fr) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Vecteurs de transformation de la levure yarrowia
EP0404097A2 (fr) 1989-06-22 1990-12-27 BEHRINGWERKE Aktiengesellschaft Récepteurs mono- et oligovalents, bispécifiques et oligospécifiques, ainsi que leur production et application
WO1991000360A1 (fr) 1989-06-29 1991-01-10 Medarex, Inc. Reactifs bispecifiques pour le traitement du sida
US5208020A (en) 1989-10-25 1993-05-04 Immunogen Inc. Cytotoxic agents comprising maytansinoids and their therapeutic use
US5416064A (en) 1989-10-25 1995-05-16 Immunogen, Inc. Cytotoxic agents comprising maytansinoids and their therapeutic use
EP0425235B1 (fr) 1989-10-25 1996-09-25 Immunogen Inc Agents cytotoxiques contenant des maytansinoides et leur application thérapeutique
US6417429B1 (en) 1989-10-27 2002-07-09 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
WO1991010741A1 (fr) 1990-01-12 1991-07-25 Cell Genesys, Inc. Generation d'anticorps xenogeniques
US5268164A (en) 1990-04-23 1993-12-07 Alkermes, Inc. Increasing blood-brain barrier permeability with permeabilizer peptides
US5112596A (en) 1990-04-23 1992-05-12 Alkermes, Inc. Method for increasing blood-brain barrier permeability by administering a bradykinin agonist of blood-brain barrier permeability
US5506206A (en) 1990-04-23 1996-04-09 Alkermes, Inc. Increasing blood-brain barrier permeability with permeabilizer peptides
WO1992000373A1 (fr) 1990-06-29 1992-01-09 Biosource Genetics Corporation Production de melanines a l'aide de microorganismes transformes
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5569825A (en) 1990-08-29 1996-10-29 Genpharm International Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
WO1992009690A2 (fr) 1990-12-03 1992-06-11 Genentech, Inc. Methode d'enrichissement pour des variantes de l'hormone de croissance avec des proprietes de liaison modifiees
US5571894A (en) 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
US6582959B2 (en) 1991-03-29 2003-06-24 Genentech, Inc. Antibodies to vascular endothelial cell growth factor
US20030203409A1 (en) 1991-03-29 2003-10-30 Genentech, Inc. Antibodies to vascular endothelial cell growth factor
US20030206899A1 (en) 1991-03-29 2003-11-06 Genentech, Inc. Vascular endothelial cell growth factor antagonists
US5686416A (en) 1991-04-23 1997-11-11 Alkermes, Inc. Increasing blood-brain barrier permeability with permeabilizer peptides
US6054297A (en) 1991-06-14 2000-04-25 Genentech, Inc. Humanized antibodies and methods for making them
US5821337A (en) 1991-06-14 1998-10-13 Genentech, Inc. Immunoglobulin variants
US6407213B1 (en) 1991-06-14 2002-06-18 Genentech, Inc. Method for making humanized antibodies
WO1993001161A1 (fr) 1991-07-11 1993-01-21 Pfizer Limited Procede de preparation d'intermediaires de sertraline
US5648237A (en) 1991-09-19 1997-07-15 Genentech, Inc. Expression of functional antibody fragments
WO1993006213A1 (fr) 1991-09-23 1993-04-01 Medical Research Council Production d'anticorps chimeriques - une approche combinatoire
US5362852A (en) 1991-09-27 1994-11-08 Pfizer Inc. Modified peptide derivatives conjugated at 2-hydroxyethylamine moieties
US5587458A (en) 1991-10-07 1996-12-24 Aronex Pharmaceuticals, Inc. Anti-erbB-2 antibodies, combinations thereof, and therapeutic and diagnostic uses thereof
WO1993008829A1 (fr) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions induisant la destruction de cellules infectees par l'hiv
WO1993016185A2 (fr) 1992-02-06 1993-08-19 Creative Biomolecules, Inc. Proteine de liaison biosynthetique pour marqueur de cancer
WO1993021232A1 (fr) 1992-04-10 1993-10-28 Research Development Foundation IMMUNOTOXINES DIRIGEES CONTRE DES ANTIGENES DE SURFACE APPARENTEES A c-erbB-2(HER-2/neu)
US20020025313A1 (en) 1992-07-27 2002-02-28 Micklus Michael J. Targeting of liposomes to the blood-brain barrier
WO1994004690A1 (fr) 1992-08-17 1994-03-03 Genentech, Inc. Immunoadhesines bispecifiques
WO1994010202A1 (fr) 1992-10-28 1994-05-11 Genentech, Inc. Antagonistes du facteur de croissance des cellules endotheliales vasculaires
EP0666868B1 (fr) 1992-10-28 2002-04-03 Genentech, Inc. Usage d'anticorps anti-VEGF pour le traitement du cancer
WO1994011026A2 (fr) 1992-11-13 1994-05-26 Idec Pharmaceuticals Corporation Application therapeutique d'anticorps chimeriques et radio-marques contre l'antigene a differentiation restreinte des lymphocytes b humains pour le traitement du lymphome des cellules b
US5635483A (en) 1992-12-03 1997-06-03 Arizona Board Of Regents Acting On Behalf Of Arizona State University Tumor inhibiting tetrapeptide bearing modified phenethyl amides
US5780588A (en) 1993-01-26 1998-07-14 Arizona Board Of Regents Elucidation and synthesis of selected pentapeptides
WO1994029351A2 (fr) 1993-06-16 1994-12-22 Celltech Limited Anticorps
US5767285A (en) 1994-06-03 1998-06-16 American Cyanamid Company Linkers useful for the synthesis of conjugates of methyltrithio antitumor agents
US5773001A (en) 1994-06-03 1998-06-30 American Cyanamid Company Conjugates of methyltrithio antitumor agents and intermediates for their synthesis
US5739116A (en) 1994-06-03 1998-04-14 American Cyanamid Company Enediyne derivatives useful for the synthesis of conjugates of methyltrithio antitumor agents
US5877296A (en) 1994-06-03 1999-03-02 American Cyanamid Company Process for preparing conjugates of methyltrithio antitumor agents
WO1996007754A1 (fr) 1994-09-02 1996-03-14 The Scripps Research Institute Procedes de production de banques d'anticorps au moyen de chaines legeres d'immunoglobuline universelles ou rendues alleatoires
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5663149A (en) 1994-12-13 1997-09-02 Arizona Board Of Regents Acting On Behalf Of Arizona State University Human cancer inhibitory pentapeptide heterocyclic and halophenyl amides
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US5840523A (en) 1995-03-01 1998-11-24 Genetech, Inc. Methods and compositions for secretion of heterologous polypeptides
WO1996027011A1 (fr) 1995-03-01 1996-09-06 Genentech, Inc. Procede d'obtention de polypeptides heteromultimeriques
WO1996030046A1 (fr) 1995-03-30 1996-10-03 Genentech, Inc. Antagonistes de facteurs de croissance des cellules endotheliales vasculaires
EP0817648A1 (fr) 1995-03-30 1998-01-14 Genentech, Inc. Antagonistes de facteurs de croissance des cellules endotheliales vasculaires
US5869046A (en) 1995-04-14 1999-02-09 Genentech, Inc. Altered polypeptides with increased half-life
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
WO1996033735A1 (fr) 1995-04-27 1996-10-31 Abgenix, Inc. Anticorps humains derives d'une xenosouris immunisee
WO1996034096A1 (fr) 1995-04-28 1996-10-31 Abgenix, Inc. Anticorps humains derives de xeno-souris immunisees
US5714586A (en) 1995-06-07 1998-02-03 American Cyanamid Company Methods for the preparation of monomeric calicheamicin derivative/carrier conjugates
US5712374A (en) 1995-06-07 1998-01-27 American Cyanamid Company Method for the preparation of substantiallly monomeric calicheamicin derivative/carrier conjugates
US20040147448A1 (en) * 1995-08-01 2004-07-29 Kari Alitalo Vascular endothelial growth factor C (VEGF-C) protein and gene, mutants thereof, and uses thereof
WO1997030087A1 (fr) 1996-02-16 1997-08-21 Glaxo Group Limited Preparation d'anticorps glycosyles
US6919436B2 (en) 1996-08-30 2005-07-19 Upfront Chromatography A/S Isolation of proteins
US20050176122A1 (en) 1996-08-30 2005-08-11 Upfront Chromatography A/S Isolation of proteins
WO1998024893A2 (fr) 1996-12-03 1998-06-11 Abgenix, Inc. MAMMIFERES TRANSGENIQUES POSSEDANT DES LOCI DE GENES D'IMMUNOGLOBULINE D'ORIGINE HUMAINE, DOTES DE REGIONS VH ET Vλ, ET ANTICORPS PRODUITS A PARTIR DE TELS MAMMIFERES
US20050026229A1 (en) 1997-03-10 2005-02-03 The Regents Of The University Of California PSCA antibodies and hybridomas producing them
WO1998045331A2 (fr) 1997-04-07 1998-10-15 Genentech, Inc. Anticorps anti-vegf
US20050112126A1 (en) 1997-04-07 2005-05-26 Genentech, Inc. Anti-VEGF antibodies
US20030190317A1 (en) 1997-04-07 2003-10-09 Genentech, Inc. Anti-VEGF antibodies
US6884879B1 (en) 1997-04-07 2005-04-26 Genentech, Inc. Anti-VEGF antibodies
US7060269B1 (en) 1997-04-07 2006-06-13 Genentech, Inc. Anti-VEGF antibodies
WO1998045332A2 (fr) 1997-04-07 1998-10-15 Genentech, Inc. Anticorps humanises et methode permettant de les produire
WO1998050431A2 (fr) 1997-05-02 1998-11-12 Genentech, Inc. Procede de preparation d'anticorps multispecifiques presentant des composants heteromultimeres
US20060183887A1 (en) 1997-05-05 2006-08-17 Abgenix, Inc. Human monoclonal antibodies to epidermal growth factor receptor
US20050100546A1 (en) 1997-05-05 2005-05-12 Abgenix, Inc. Human monoclonal antibodies to epidermal growth factor receptor
WO1998058964A1 (fr) 1997-06-24 1998-12-30 Genentech, Inc. Procedes et compositions concernant des glycoproteines galactosylees
WO1999022764A1 (fr) 1997-10-31 1999-05-14 Genentech, Inc. Compositions renfermant des glycoformes de glycoproteine et methodes afferentes
US7189826B2 (en) 1997-11-24 2007-03-13 Institute For Human Genetics And Biochemistry Monoclonal human natural antibodies
US7087409B2 (en) 1997-12-05 2006-08-08 The Scripps Research Institute Humanization of murine antibody
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
WO1999051642A1 (fr) 1998-04-02 1999-10-14 Genentech, Inc. Variants d'anticorps et fragments de ceux-ci
US6602684B1 (en) 1998-04-20 2003-08-05 Glycart Biotechnology Ag Glycosylation engineering of antibodies for improving antibody-dependent cellular cytotoxicity
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
WO2000029004A1 (fr) 1998-11-18 2000-05-25 Peptor Ltd. Petites unites fonctionnelles de regions variables a chaine lourde d'anticorps
US6737056B1 (en) 1999-01-15 2004-05-18 Genentech, Inc. Polypeptide variants with altered effector function
WO2000042072A2 (fr) 1999-01-15 2000-07-20 Genentech, Inc. Variants polypeptidiques ayant une fonction effectrice alteree
US20060059575A1 (en) 1999-03-30 2006-03-16 Japan Tobacco, Inc. Method for preparing monoclonal antibody
WO2000061739A1 (fr) 1999-04-09 2000-10-19 Kyowa Hakko Kogyo Co., Ltd. Methode de regulation de l'activite d'une molecule immunologiquement fonctionnelle
US6703020B1 (en) 1999-04-28 2004-03-09 Board Of Regents, The University Of Texas System Antibody conjugate methods for selectively inhibiting VEGF
US6420548B1 (en) 1999-10-04 2002-07-16 Medicago Inc. Method for regulating transcription of foreign genes
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
WO2001029246A1 (fr) 1999-10-19 2001-04-26 Kyowa Hakko Kogyo Co., Ltd. Procede de production d'un polypeptide
US20030055006A1 (en) 2000-06-23 2003-03-20 Schering Aktiengesellschaft Combinations and compositions which interfere with VEGF/VEGF and angiopoietin/tie receptor function and their use
US20020038086A1 (en) 2000-07-27 2002-03-28 Hynynen Kullervo H. Blood-brain barrier opening
US20020065259A1 (en) 2000-08-30 2002-05-30 Schatzberg Alan F. Glucocorticoid blocking agents for increasing blood-brain barrier permeability
US20030115614A1 (en) 2000-10-06 2003-06-19 Yutaka Kanda Antibody composition-producing cell
WO2002031140A1 (fr) 2000-10-06 2002-04-18 Kyowa Hakko Kogyo Co., Ltd. Cellules produisant des compositions d'anticorps
US20020164328A1 (en) 2000-10-06 2002-11-07 Toyohide Shinkawa Process for purifying antibody
US20030073713A1 (en) 2000-10-30 2003-04-17 Schoenhard Grant L. Inhibitors of ABC drug transporters at the blood-brain barrier
US20030083299A1 (en) 2000-11-04 2003-05-01 Ferguson Ian A. Non-invasive delivery of polypeptides through the blood-brain barrier
WO2002051870A2 (fr) 2000-12-22 2002-07-04 GRAD, Carole Legal Representative of KAPLAN, Howard Bibliotheques d'affichage de phages de fragments vh humains
WO2002088172A2 (fr) 2001-04-30 2002-11-07 Seattle Genetics, Inc. Composes pentapeptidiques et leurs utilisations
US20040131692A1 (en) 2001-05-05 2004-07-08 Joerg Kreuter Nanoparticles made of protein with coupled apolipoprotein e for penetration of the blood-brain barrier and methods for the production thereof
US7078492B2 (en) 2001-05-16 2006-07-18 Abgenix, Inc. Human antipneumococcal antibodies from non-human animals
US20030129186A1 (en) 2001-07-25 2003-07-10 Biomarin Pharmaceutical Inc. Compositions and methods for modulating blood-brain barrier transport
WO2003011878A2 (fr) 2001-08-03 2003-02-13 Glycart Biotechnology Ag Variants de glycosylation d'anticorps presentant une cytotoxicite cellulaire accrue dependante des anticorps
US7153507B2 (en) 2001-08-23 2006-12-26 Genmab A/S Human antibodies specific for interleukin 15 (IL-15)
WO2003035694A2 (fr) 2001-10-24 2003-05-01 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Anticorps fonctionnels a chaine lourde, fragments de ces derniers, bibliotheque de ces derniers et procedes de production
US20030157108A1 (en) 2001-10-25 2003-08-21 Genentech, Inc. Glycoprotein compositions
US20040093621A1 (en) 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
US20030162695A1 (en) 2002-02-27 2003-08-28 Schatzberg Alan F. Glucocorticoid blocking agents for increasing blood-brain barrier permeability
US20050124533A1 (en) 2002-02-27 2005-06-09 Schatzberg Alan F. Glucocorticoid blocking agents for increasing blood-brain barrier permeability stan-261con
WO2003085107A1 (fr) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Cellules à génome modifié
US20040132140A1 (en) 2002-04-09 2004-07-08 Kyowa Hakko Kogyo Co., Ltd. Production process for antibody composition
WO2003084570A1 (fr) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Medicament contenant une composition d'anticorps appropriee au patient souffrant de polymorphisme fc$g(g)riiia
US20040110704A1 (en) 2002-04-09 2004-06-10 Kyowa Hakko Kogyo Co., Ltd. Cells of which genome is modified
WO2003085119A1 (fr) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Procede d'amelioration de l'activite d'une composition d'anticorps de liaison avec le recepteur fc$g(g) iiia
US20040110282A1 (en) 2002-04-09 2004-06-10 Kyowa Hakko Kogyo Co., Ltd. Cells in which activity of the protein involved in transportation of GDP-fucose is reduced or lost
US20040109865A1 (en) 2002-04-09 2004-06-10 Kyowa Hakko Kogyo Co., Ltd. Antibody composition-containing medicament
EP1391213A1 (fr) 2002-08-21 2004-02-25 Boehringer Ingelheim International GmbH Compositions et méthodes pour le traitement du cancer en utilisant un conjugué d'un anticorps contre le CD44 avec un maytansinoide et des agents chimiothérapeutiques
US20050014934A1 (en) 2002-10-15 2005-01-20 Hinton Paul R. Alteration of FcRn binding affinities or serum half-lives of antibodies by mutagenesis
US20040204354A1 (en) 2002-12-03 2004-10-14 Thomas Nelson Artificial low-density lipoprotein carriers for transport of substances across the blood-brain barrier
WO2004056312A2 (fr) 2002-12-16 2004-07-08 Genentech, Inc. Variants d'immunoglobuline et utilisations
US20060258841A1 (en) 2003-01-17 2006-11-16 Josef Michl Pancreatic cancer associated antigen, antibody thereto, and diagnostic and treatment methods
WO2004092219A2 (fr) 2003-04-10 2004-10-28 Protein Design Labs, Inc Modification d'affinites de liaison pour fcrn ou de demi-vies de serum d'anticorps par mutagenese
US20050287149A1 (en) 2003-05-21 2005-12-29 Medarex, Inc. Human monoclonal antibodies against bacillus anthracis protective antigen
WO2004113304A1 (fr) 2003-05-22 2004-12-29 Abbott Laboratories Inhibiteurs de kinases de type indazole, benzisoxazole et benzisothiazole
US20050186208A1 (en) 2003-05-30 2005-08-25 Genentech, Inc. Treatment with anti-VEGF antibodies
US20060280747A1 (en) 2003-08-01 2006-12-14 Genentech, Inc. Anti-VEGF antibodies
WO2005012359A2 (fr) 2003-08-01 2005-02-10 Genentech, Inc. Anticorps anti-vegf
US20070141065A1 (en) 2003-08-01 2007-06-21 Genentech, Inc. Anti-VEGF antibodies
US20070020267A1 (en) 2003-08-01 2007-01-25 Genentech, Inc. Anti-VEGF antibodies
US20050089473A1 (en) 2003-09-10 2005-04-28 Cedars-Sinai Medical Center Potassium channel mediated delivery of agents through the blood-brain barrier
WO2005035572A2 (fr) 2003-10-08 2005-04-21 Domantis Limited Compositions d'anticorps et procedes
WO2005035586A1 (fr) 2003-10-08 2005-04-21 Kyowa Hakko Kogyo Co., Ltd. Composition proteique hybride
WO2005035778A1 (fr) 2003-10-09 2005-04-21 Kyowa Hakko Kogyo Co., Ltd. Procede permettant de produire une composition d'anticorps par inhibition par l'arn de la fonction de $g(a)1,6-fucosyltransferase
US20050123546A1 (en) 2003-11-05 2005-06-09 Glycart Biotechnology Ag Antigen binding molecules with increased Fc receptor binding affinity and effector function
WO2005053742A1 (fr) 2003-12-04 2005-06-16 Kyowa Hakko Kogyo Co., Ltd. Medicament contenant une composition a base d'anticorps
US20060009360A1 (en) 2004-06-25 2006-01-12 Robert Pifer New adjuvant composition
US20080181888A1 (en) 2004-12-31 2008-07-31 Ambrose Christine M Polypeptides That Bind Br3 and Uses Thereof
WO2007056470A2 (fr) 2005-11-08 2007-05-18 Genentech, Inc. Antagonistes de la neuropiline
US20090142343A1 (en) 2007-11-30 2009-06-04 Genentech, Inc. Anti-VEGF antibodies
WO2009073160A1 (fr) 2007-11-30 2009-06-11 Genentech, Inc. Anticorps anti-vegf

Non-Patent Citations (340)

* Cited by examiner, † Cited by third party
Title
"Antibodies: A Practical Approach", 1988, IRL PRESS
"Guide for the Care and Use of Laboratory Animals", 1985, NIH
"Short Protocols in Molecular Biology", 1999, WILEY AND SONS
A. DOYLE, J. B. GRIFFITHS, AND D. G. NEWELL,: "Cell and Tissue Culture: Laboratory Procedures", August 1993, J. WILEY AND SONS
A. L. LEHNINGER: "Biochemistry", 1975, WORTH PUBLISHERS, pages: 73 - 75
ABBAS ET AL.: "Cellular and Mol. Immunology, 4th ed.", 2000, W.B. SAUNDERS, CO.
ACHEN ET AL., BR J CANCER, vol. 94, 2006, pages 1355 - 1360
AGNEW, CHEM INTL. ED. ENGL., vol. 33, 1994, pages 183 - 186
AIELLO ET AL., N. ENGL. J. MED., vol. 331, 1994, pages 1480 - 1487
ALBUQUERQUE ROMULO J C ET AL: "Alternatively spliced vascular endothelial growth factor receptor-2 is an essential endogenous inhibitor of lymphatic vessel growth.", NATURE MEDICINE SEP 2009 LNKD- PUBMED:19668192, vol. 15, no. 9, September 2009 (2009-09-01), pages 1023 - 1030, XP007915764, ISSN: 1546-170X *
ALITALO; CARMELIET, CANCER CELL, vol. 1, 2002, pages 219 - 227
BALDWIN ET AL., LANCET, 15 March 1986 (1986-03-15), pages 603 - 05
BARBAS ET AL., PROC NAT. ACAD. SCI. USA, vol. 91, 1994, pages 3809 - 3813
BARBAS ET AL., PROC. NATL. ACAD. SCI USA, vol. 88, 1991, pages 7978 - 7982
BARBAS ET AL., PROC. NATL. ACAD. SCI. USA, vol. 88, 1991, pages 7978 - 7982
BARBAS ET AL., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 4457 - 4461
BARNES ET AL., ANAL. BIOCHEM., vol. 102, 1980, pages 255
BASS ET AL., PROTEINS, vol. 8, 1990, pages 309 - 314
BEHRSTOCK ET AL., GENE THER., 15 December 2005 (2005-12-15)
BERKMAN ET AL., J. CLIN. INVEST., vol. 91, 1993, pages 153 - 159
BOBO ET AL., PROC. NATL. ACAD. SCI. USA, vol. 91, 1994, pages 2076 - 2080
BOEMER ET AL., J. IMMUNOL., vol. 147, no. 1, 1991, pages 86 - 95
BOERNER ET AL., J. IMMUNOL., vol. 147, 1991, pages 86
BORGSTR6M ET AL., CANCER RES., vol. 56, 1996, pages 4032 - 4039
BORREBAECK,: "Antibody Engineering", article "The antibody fragment may also be a "linear antibody"
BRCNNAN ET AL., SCIENCE, vol. 229, 1985, pages 81
BRENNAN ET AL., SCIENCE, vol. 229, 1985, pages 81
BRODEUR ET AL.: "Monoclonal Antibody Production Techniques and Applications", 1987, MARCCL DEKKER, pages: 51 - 63
BRODEUR ET AL.: "Monoclonal Antibody Production Techniques and Applications", 1987, MARCEL DCKKCR, INC., pages: 51 - 63
BROWN ET AL., CANCER RES., vol. 53, 1993, pages 4727 - 4735
BROWN ET AL., HUMAN PATHOL., vol. 26, 1995, pages 86 - 91
BRUGGEMANN ET AL., YEAR IN IMMUNOL., vol. 7, 1993, pages 33
BRUGGEMANN, M. ET AL., J. EXP. MED., vol. 166, 1987, pages 1351 - 1361
BRUGGERMANN ET AL., YEAR IN IMMUNOL., vol. 7, 1993, pages 33
C. A. JANEWAY; P. TRAVERS, IMMUNOBIOLOGY, 1997
C. V ET AL., BLOOD, vol. 108, 2006, pages 3103 - 3111
CAO ET AL., PNAS, vol. 95, 1998, pages 14389 - 14394
CAPEL ET AL., IMMUNOMETHODS, vol. 4, 1994, pages 25 - 34
CARLSSON ET AL., BIOCHEM. J., vol. 173, 1978, pages 723 - 737
CARMELIET; JAIN, NATURE, vol. 407, 2000, pages 249 - 257
CARTER ET AL., BIO/TECHNOLOGY, vol. 10, 1992, pages 163 - 167
CARTER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 4285
CHAMPE ET AL., J. BIOL. CHEM., vol. 270, 1995, pages 1388 - 1394
CHAMPE ET AL.: "J. Biol. Chem.", vol. 270, 1995, pages: 1388 - 1394
CHARI ET AL., CANCER RESEARCH, vol. 52, 1992, pages 127 - 131
CHARLTON: "Methods in Molecular Biology", vol. 248, 2003, HUMANA PRESS, pages: 245 - 254
CHATAL: "Monoclonal Antibodies in Immunoscintigraphy", 1989, CRC PRESS
CHEN ET AL., J. MOL. BIOL., vol. 293, 1999, pages 865 - 881
CHEN, CANCER RES, vol. 65, 2005, pages 9004 - 9011
CHOTHIA ET AL., J. MOL. BIOL, vol. 196, 1987, pages 901
CHOTHIA; LESK, J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
CLACKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 628
CLYNES ET AL., PNAS, vol. 95, 1998, pages 652 - 656
CLYNES ET AL., PROC. NAT'LACAD. SCI. USA, vol. 95, 1998, pages 652 - 656
COLE ET AL.: "Monoclonal Antibodies and Cancer Therapy", 1985, ALAN R. LISS, pages: 77
CONNOLLY, J. APPL. CRYST., vol. 16, 1983, pages 548
CRAGG; M.S., BLOOD, vol. 101, 2003, pages 1045 - 1052
CRAGG; M.S.; M.J. GLENNIE, BLOOD, vol. 103, 2004, pages 2738 - 2743
CUNNINGHAM; WELLS, SCIENCE, vol. 244, 1989, pages 1081 - 1085
D. M. WEIR AND C. C. BLACKWCLL: "Gene Transfer Vectors for Mammalian Cells", 1987, article "Handbook of Experimental Immunology"
DAERON, ANNU. REV.LMMUNOL., vol. 15, 1997, pages 203 - 234
DAMIS ET AL., ARCH. OPHTHALMOL., vol. 114, 1996, pages 66 - 71
DAVID ET AL., BIOCHEMISTRY, vol. 13, 1974, pages 1014 - 1021
DEV COMP IMMUNOL, vol. 30, 2006, pages 43 - 56
DIJK; VAN DE WINKEL, CURR. OPIN. PHARMACOL., vol. 5, 2001, pages 368 - 74
DORONINA ET AL., NATURE BIOTECHNOL., vol. 21, no. 7, 2003, pages 778 - 784
DORONINA, NAT BIOTECHNOL, vol. 21, no. 7, 2003, pages 778 - 784
DRUGS OF THE FUTURE, vol. 25, no. 7, 2000, pages 686
DUNCAN; WINTER, NATURE, vol. 322, 1988, pages 738 - 40
DVORAK ET AL., AM. J. PATHOL., vol. 146, 1995, pages 1029 - 1039
E. HARLOW AND D. LANE: "Antibodies: A Laboratory Manual", 1999, COLD SPRING HARBOR LABORATORY PRESS
E. SCHRÖDER; K. LILBKE: "The Peptides", vol. 1, 1965, ACADEMIC PRESS, pages: 76 - 136
ED HARLOW AND DAVID LANE: "Antibodies", 1988, COLD SPRING HARBOR LABORATORY
ED HARLOW; DAVID LANE: "Antibodies, A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
EMBLETON ET AL., NUCL. ACIDS RES., vol. 20, 1992, pages 3831 - 3837
EVEN ET AL., TRENDS IN BIOTECHNOLOGY, vol. 24, no. 3, 2006, pages 105 - 108
F. M. AUSUBEL, ET AL.: "CURRENT PROTOCOLS IN MOLECULAR BIOLOGY", 2003
FEBS LETT, vol. 339, 1994, pages 285 - 290
FELLOUSE, PROC. NATL. ACAD. SCI. USA, vol. 101, no. 34, 2004, pages 12467 - 12472
FERRARA ET AL., ENDOCR. REV.
FERRARA ET AL., ENDOCR. REV., vol. 18, 1997, pages 4 - 25
FERRARA ET AL., NAT MED, vol. 9, 2003, pages 669 - 676
FERRARA ET AL., NATURE REVIEWS:DRUG DISCOVERY, vol. 3, 2004, pages 391 - 400
FERRARA; ALITALO, NATURE MEDICINE, vol. 5, no. 12, 1999, pages 1359 - 1364
FISHWILD ET AL., NATURE BIOTECHNOL., vol. 14, 1996, pages 845 - 851
FLEER ET AL., BIO/TECHNOLOGY, vol. 9, 1991, pages 968 - 975
FOLKMAN ET AL., J. BIOL. CHEM., vol. 267, 1992, pages 10931 - 10934
FOLKMAN ET AL., NATURE, vol. 339, 1989, pages 58
FOLKMAN, NAT MED, vol. 1, no. 1, 1995, pages 27 - 31
FRAKE ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 80, 1978, pages 49 - 57
FRANEK, TRENDS IN MONOCLONAL ANTIBODY RESEARCH, 2005, pages 111 - 122
GARNER A.: "Pathobiology of Ocular Disease. A Dynamic Approach, 2nd Edition", 1994, MARCEL DEKKER, article "Vascular diseases", pages: 1625 - 1710
GARRARD; HENNER, GENE, vol. 128, 1993, pages 103
GAZZANO-SANTORO ET AL., J. IMMUNOL. METHODS, vol. 202, 1996, pages 163
GEARING ET AL., EMBO J., vol. 8, 1989, pages 3667 - 3676
GEMGROSS, NAT. BIOTECH., vol. 22, 2004, pages 1409 - 1414
GEOGHEGAN; STROH, BIOCONJUGATE CHEM., vol. 3, 1992, pages 138 - 146
GHETIE ET AL., NATURE BIOTECHNOLOGY, vol. 15, no. 7, 1997, pages 637 - 640
GHETIE; WARD., IMMUNOL. TODAY, vol. 18, no. 12, 1997, pages 592 - 598
GILL ET AL., NATURE MED., vol. 9, 2003, pages 589 - 595
GODING: "Monoclonal Antibodies: Principles and Practice", 1986, ACADEMIC PRESS, pages: 59 - 103
GOODWIN ET AL., CELL, vol. 73, 1993, pages 447 - 456
GRAHAM ET AL., J. GEN VIROL., vol. 36, 1977, pages 59
GRIFFITHS ET AL., EMBO J, vol. 12, 1993, pages 725 - 734
GRUBCR ET AL., J. IMMUNOL., vol. 152, 1994, pages 5368
GUPTA; MASSAGUE, CELL, vol. 127, 2006, pages 679 - 695
GUSS ET AL., EMBOJ., vol. 5, 1986, pages 15671575
GUYER ET AL., J. IMMUNOL., vol. 117, no. 587, 1976
GUYER ET AL., J. LMMUNOL., vol. 117, 1976, pages 587
H ET AL., J. NATL CANCER INST, vol. 94, 2002, pages 8190825
HAAS ET AL., J. LAB. CLIN. MED., vol. 126, 1995, pages 330 - 41
HAM ET AL., METH. ENZ., vol. 58, 1979, pages 44
HAMERS-CASTERMAN ET AL., NATURE, vol. 363, 1993, pages 446 - 448
HAMMCRLING ET AL.: "in: Monoclonal Antibodies and T-Cell Hybridomas", 1981, ELSEVIER, pages: 563 - 681
HAMMERLING ET AL.: "Monoclonal Antibodiev and T-Cell Hybridomas", 1981, ELSEVIER, pages: 563 - 681
HANAHAN, D., SCIENCE, vol. 277, 1997, pages 48 - 50
HARLOW AND LANE,: "ANTIBODIES, A LABORATORY MANUAL", 1988
HARLOW ET AL.: "Antibodies: A Laboratory Manual, 2nd ed.", 1988, COLD SPRING HARBOR LABORATORY PRESS
HARRIS, BIOCHEM. SOC. TRANSACTIONS, vol. 23, 1995, pages 1035 - 1038
HAWKINS ET AL., J. MOL. BIOL., vol. 226, 1992, pages 889 - 896
HELDIN, CELL, vol. 80, 1995, pages 213 - 223
HELLSTROM, I ET AL., PROC. NAT'L ACAD. SCI. USA, vol. 82, 1985, pages 1499 - 1502
HELLSTROM, I. ET AL., PROC. NAT'L ACAD. SCI. USA, vol. 83, 1986, pages 7059 - 7063
HINMAN ET AL., CANCER RES., vol. 53, 1993, pages 3336 - 3342
HINMAN ET AL., CANCER RESEARCH, vol. 53, 1993, pages 3336 - 3342
HINTON ET AL., J. BIOL. CHEM., vol. 279, no. 8, 2004, pages 6213 - 6216
HOGAN, B. L.; KOLODZIEJ, P. A., NATURE REVIEWS GENETICS, vol. 3, 2002, pages 513 - 23
HOGREFE ET AL., GENE, vol. 128, no. 1, 1993, pages 19 - 126
HOLLINGER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 6444 - 6448
HONGO ET AL., HYBRIDOMA, vol. 14, no. 3, 1995, pages 253 - 260
HOOGENBOOM ET AL., NUCL. ACIDS RES., vol. 19, 1991, pages 4133 - 4137
HOOGENBOOM ET AL.: "Methods in Molecular Biology", vol. 178, 2001, HUMAN PRESS, pages: 1 - 37
HOOGENBOOM; WINTER, J. MOL. BIOL., vol. 227, 1991, pages 381
HOOGENBOOM; WINTER, J. MOL. BIOL., vol. 227, 1992, pages 381 - 388
HOOGENBOOM; WINTER, MOL. BIOL., vol. 227, 1992, pages 381 - 388
HORAK ET AL., LANCET, vol. 340, 1992, pages 1120 - 1124
HOUCK ET AL., MOL. ENDOCRIN, vol. 5, 1991, pages 1806
HUDSON ET AL., NAT. MED., vol. 9, 2003, pages 129 - 134
HUNTER ET AL., NATURE, vol. 144, 1962, pages 945
HURLE; GROSS, CURR. OP. BIOTECH., vol. 5, 1994, pages 428 - 433
IDUSOGI ET AL., J. IMMUNOL., vol. 164, 2000, pages 4178 - 4184
IDUSOGIE ET AL., J. IMMUNOL., vol. 164, 2000, pages 4178 - 4184
J. E. CELLIS,: "Cell Biology: A Laboratory Notebook", 1998, ACADEMIC PRESS
J. E. COLIGAN ET AL.,: "Current Protocols in Immunology", 1991
J. P. MATHER; P. E. ROBERTS: "Introduction to Cell and Tissue Culture", 1998, PLENUM PRESS
JACKSON ET AL., J. IMMUNOL., vol. 154, no. 7, 1995, pages 3310 - 9
JAKOBOVITS ET AL., NATURE, vol. 362, 1993, pages 255
JAKOBOVITS ET AL., NATURE, vol. 362, 1993, pages 255 - 258
JAKOBOVITS ET AL., PROC. NATL. ACAD. SCI USA, vol. 90, 1993, pages 2551
JAKOBOVITS ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 2551
JOHNSON; WU: "Methods in Molecular Biology", vol. 248, 2003, HUMAN PRESS, pages: 1 - 25
JONES ET AL., BIOTECHNOL., vol. 9, 1991, pages 88 - 89
JONES ET AL., NATURE, vol. 321, 1986, pages 522 - 525
JONES, GENETICS, vol. 85, 1977, pages 12
K. LACTIS.; VAN DEN BERG, BIOLTECHNOLOGY, vol. 8, 1990, pages 135
KABAT ET AL.: "Sequences of Immunological Interest. 5th Ed.", 1991, PUBLIC HEALTH SERVICE, NATIONAL INSTITUTES OF HEALTH
KABAT ET AL.: "Sequences of Proteins of Immunological Interest, Fifth Edition", vol. 1-3, 1991, NIH PUBLICATION 91-3242
KABAT ET AL.: "Sequences of Proteins of Immunological Interest,5th Ed.", 1991, PUBLIC HEALTH SERVICE, NATIONAL INSTITUTES OF HEALTH
KABAT ET AL.: "Sequences uf Proteins of Immunologicul Interest,, Fifth Edition,", 1991, NATIONAL INSTITUTE OF HEALTH, BETHESDA
KAM ET AL., PROC. NATL. ACAD. SCI. USA, vol. 102, 2005, pages 11600 - 11605
KANDA, Y. ET AL., BIOTECHNOL. BIOENG., vol. 94, no. 4, 2006, pages 680 - 688
KARPANEN ET AL., FASEB J, vol. 20, 2001, pages 1462 - 1472
KIM ET AL., J. IMMUNOL., vol. 24, 1994, pages 249
KIM ET AL., NATURE, vol. 362, 1993, pages 841 - 844
KLAGSBRUN ET AL., ANNU. REV. PHYSIOL., vol. 53, 1991, pages 217 - 239
KLAGSBRUN; D'AMORC, ANNU. REV. PHYSIOL., vol. 53, 1991, pages 217 - 39
KLAGSBRUN; D'AMORE, ANNU. REV. PHYSIOL., vol. 53, 1991, pages 217 - 39
KNAPPEK ET AL., J. MOL. BIOL., vol. 296, 1999, pages 57 - 86
KOHLER ET AL., NATURE, vol. 256, 1975, pages 495
KOHLER; MILSTEIN, NATURE, vol. 256, 1975, pages 495 - 97
KOSTELNY ET AL., J. IMMUNOL., vol. 148, no. 5, 1992, pages 1547 - 1553
KOZBOR, J. IMMUNOL., vol. 133, 1984, pages 3001
KRISHNAN, CANCER RES, vol. 63, 2003, pages 713 - 722
LAMBERT, J., CURR. OPINION IN PHANNACOLOGY, vol. 5, 2005, pages 543 - 549
LEE ET AL., J. IMMUNOL. METHODS, vol. 284, no. 1-2, 2004, pages 119 - 132
LEE ET AL., J. MOL. BIOL., vol. 340, no. 5, 2004, pages 1073 - 1093
LEE ET AL., J. MOL. BIOL., vol. 340, no. 5, 2004, pages 1073 - 93
LEE; C. V. ET AL., JMOI BIOL, vol. 340, 2004, pages 1073 - 93
LEE; CV, J MOL BIOL, vol. 340, 2004
LEE; RICHARDS, J. MOL. BIOL., vol. 55, 1971, pages 379
LEONG ET AL., APPL. IMMUNOHISTOCHEM., vol. 4, no. 3, 1996, pages 201
LEUNG ET AL., SCIENCE, vol. 246, 1989, pages 1306
LEUNG ET AL., TECHNIQUE, vol. 1, 1989, pages 11 - 15
LI ET AL., NAT. BIOTECH., vol. 24, 2006, pages 210 - 215
LI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 103, 2006, pages 3557 - 3562
LIANG ET AL., J MOL BIOL, vol. 366, 2007, pages 815 - 829
LIANG ET AL., JBIOL CHEM, vol. 281, 2006, pages 951 - 961
LIANG W-C ET AL: "Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 281, no. 2, 13 January 2006 (2006-01-13), pages 951 - 961, XP002481172, ISSN: 0021-9258, [retrieved on 20051107], DOI: DOI:10.1074/JBC.M508199200 *
LIN, CANCER RES, vol. 65, 2005, pages 6901 - 6909
LINDMARK ET AL., J. IMMUNOL. METH., vol. 62, 1983, pages 1 - 13
LIU ET AL., PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 8618 - 8623
LODE ET AL., CANCER RES., vol. 58, 1998, pages 2928
LODE ET AL., CANCER RESEARCH, vol. 58, 1998, pages 2925 - 2928
LONBERG ET AL., NATURE, vol. 368, 1994, pages 856 - 859
LONBERG; HUSZA, INTERN. REV. IMMUNOL., vol. 13, 1995, pages 65 - 93
LOPEZ ET AL., INVEST. OPHTHALMOL. VIS. SCI., vol. 37, 1996, pages 855 - 868
LOWMAN; WELLS, METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, vol. 3, 1991, pages 205 - 0216
LUBARSKY, B.; KRASNOW, M. A., CELL, vol. 112, 2003, pages 19 - 28
LYMAN ET AL., CELL, vol. 75, 1993, pages 1157 - 1167
M. J. GAIT,: "Methods in Molecular Biology", 1984, HUMANA PRESS, article "Oligonucleotide Synthesis"
M. J. MACPHERSON, B. D. HAMES AND G. R. TAYLOR: "PCR 2: A PRACTICAL APPROACH", 1995
M. JOHNSTON AND STAN FIELDS: "METHODS IN ENZYMOLOGY", ACADEMIC PRESS, INC.
M. ZANETTI AND J. D. CAPRA,: "The Antibodies", 1995, HARWOOD ACADEMIC
MACCHIARINI ET AL., LANCET, vol. 340, 1992, pages 145 - 146
MANCUSO ET AL., J CLIN INVEST, vol. 116, no. 10, 2006, pages 2585 - 7
MANDLER ET AL., BIOCONJUGATE CHEM., vol. 13, 2002, pages 786 - 791
MANDLER ET AL., BIOORGANIC & MED. CHEM. LETTERS, vol. 10, 2000, pages 1025 - 1028
MANDLER ET AL., J. NAT. CANCER INST., vol. 92, no. 19, 2000, pages 1573 - 1581
MANDRIOTA ET AL., EMBO J, vol. 20, 2001, pages 672 - 682
MARKS ET AL., BIO/TECHNOLOGY, vol. 10, 1992, pages 779 - 783
MARKS ET AL., BIOTECHNOL., vol. 10, 1992, pages 779 - 783
MARKS ET AL., J. MOL. BIOL., vol. 222, 1991, pages 581
MARKS ET AL., J. MOL. BIOL., vol. 222, 1991, pages 581 - 597
MARKS ET AL., J. MOL. BIOL., vol. 222, 1992, pages 581 - 597
MATHER ET AL., ANNALS N. Y. ACAD. SCI., vol. 383, 1982, pages 44 - 68
MATHER, BIOL. REPROD., vol. 23, 1980, pages 243 - 251
MATSUDA ET AL., NATURE GENET., vol. 3, 1993, pages 88 - 94
MATTERN ET AL., BRIT. J. CANCER, vol. 73, 1996, pages 931 - 934
MELNYK ET AL., CANCER RES., vol. 56, 1996, pages 921 - 924
MILSTEIN; CUELLO, NATURE, vol. 305, 1983, pages 537
MORIMOTO ET AL., JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, vol. 24, 1992, pages 107 - 117
MORRIS: "Methods in Molecular Biology", vol. 66, 1996, HUMANA PRESS, article "Epitope Mapping Protocols"
MORRISON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 6851 - 6855
MORRISON, NATURE, vol. 368, 1994, pages 812 - 813
MULLIS ET AL.,: "PCR: The Polymerase Chain Reaction", 1994
MUNSON ET AL., ANAL. BIOCHEM., vol. 107, 1980, pages 220
MURAKAMI ET AL.: "The Molecular Basis of Cancer", 1995, W.B. SAUNDERS, article "Cell cycle regulation, oncogenes, and antineoplastic drugs"
NATHANSON, CANCER, vol. 98, 2003, pages 413 - 423
NATURE, vol. 341, 1989, pages 544 - 546
NEUBERGER, NATURE BIOTECHNOL., vol. 14, 1996, pages 826
NEUWELT, E. A.: "Implication of the Blood-Brain Barrier and its Manipulation", vol. 1, 2, 1989, PLENUM PRESS
NI, XIANDAI MIANYIXUE, vol. 26, no. 4, 2006, pages 265 - 268
NICULESCU-DUVAZ; SPRINGER, ADV. DRUG DELIV. REV., vol. 26, 1997, pages 151 - 172
NYGREN, J. HISTOCHENT. AND CYTOCHENZ., vol. 30, 1982, pages 407 - 412
OKAZAKI ET AL., J. MOL. BIOL., vol. 336, 2004, pages 1239 - 1249
ORLANDI ET AL., PROC. NATL. ACAD. SCI. (LISA), vol. 86, 1989, pages 3833 - 3837
ORLANDI; WARD ET AL., NATURE, vol. 341, 1989, pages 544 - 546
ORUM ET AL., NUCLEIC ACIDS RES., vol. 21, 1993, pages 4491 - 4498
O'SULLIVAN ET AL.: "Methods in Enzymology", vol. 73, 1981, ACADEMIC PRESS, article "Methods for the Preparation of Enzyme-antibody Conjugates for Use in Enzyme Immunoassay", pages: 147 - 166
P. FINCH, ANTIBODIES, 1997
P. SHEPHERD AND C. DEAN,: "Monoclonal Antibodies: A Practical Approach", 2000, OXFORD UNIVERSITY PRESS
PACIOS, COMPUT. CHEM., vol. 18, no. 4, 1994, pages 377 - 386
PAIN ET AL., J. IMMUNOL. METHODS, vol. 40, 1981, pages 219 - 230
PAPANASTASSIOU ET AL., GENE THERAPY, vol. 9, 2002, pages 398 - 406
PETKOVA, S.B. ET AL., INT'L. IMMUNOL., vol. 18, no. 12, 2006, pages 1759 - 1769
PETTIT ET AL., ANTI-CANCER DRUG DESIGN, vol. 13, 1998, pages 243 - 277
PETTIT ET AL., ANTIMICROB. AGENTS CHEMOTHER., vol. 42, 1998, pages 2961 - 2965
PETTIT ET AL., J. AM. CHEM. SOC., vol. 111, 1989, pages 5463 - 5465
PETTIT ET AL., J. CHEM. SOC. PERKIN TRANS., vol. 1, no. 5, 1996, pages 859 - 863
PETTIT, G.R. ET AL., SYNTHESIS, 1996, pages 719 - 725
PLUCKTHUN, IMMUNOL. REVS, vol. 130, 1992, pages 151
PLUCKTHUN: "The Phannacology of Monoclonal Antibodies", vol. 113, 1994, SPRINGER-VERLAG, pages: 269 - 315
POLVERINI ET AL., METHODS ENZYMOL, vol. 198, 1991, pages 440 - 450
POPKOV ET AL., JOURNAL OF IMMUNOLOGICAL METHODS, vol. 288, 2004, pages 149 - 164
POPKOV ET AL., JOURNAL OFLMMUNOLOGICAL METHODS, vol. 288, 2004, pages 149 - 164
PRESTA ET AL., CANCER RES., vol. 57, 1997, pages 4593 - 4599
PRESTA ET AL., J. IMMUNOL., vol. 151, 1993, pages 2623
PRESTA, CURR. OP. STRUCT. BIOL., vol. 2, 1992, pages 593 - 596
PROC. NATL. ACAD. SCI USA, vol. 89, 1992, pages 3576 - 3580
PROC. NATL. ACAD. SCI. USA, vol. 93, pages 8618 - 8623
R. 1. FRESHNEY,: "ANIMAL CELL CULTURE", 1987
R. I. FRESHNEY: "Animal C'ell Culture", 1987
RAN SOPHIA ET AL: "Generation of new rabbit monoclonal antibody RAM-1 against human VEGF-C", PROCEEDINGS OF THE ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 46, 20 April 2005 (2005-04-20), pages 911, XP001539565, ISSN: 0197-016X *
RAVETCH; KINET, ANNU. REV. IMMUNOL, vol. 9, 1991, pages 457 - 92
RAVETCH; KINET, ANNU. REV. IMMUNOL., vol. 9, 1991, pages 457 - 92
REMINGTON: "The Science and Practice of Pharmacy 20th edition", 2000
REYES ET AL., NATURE, vol. 297, 1982, pages 598 - 601
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323 - 327
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323 - 329
RINDERKNECHT MATTHIAS ET AL: "Phage-derived fully human monoclonal antibody fragments to human vascular endothelial growth factor-C block its interaction with VEGF receptor-2 and 3.", PLOS ONE 2010 LNKD- PUBMED:20689828, vol. 5, no. 8, 2010, pages E11941, XP007915762, ISSN: 1932-6203 *
RIPKA ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 249, 1986, pages 533 - 545
ROWLAND ET AL., CANCER IMMUNOL. IMMUNOTHER., vol. 21, 1986, pages 183 - 87
SAMBROOK: "Molecular Cloning: A Laboratory Manual 3rd. edition", 2001, COLD SPRING HARBOR LABORATORY PRESS
SASTRY ET AL., PROC. NATL. ACAD. SCI. (USA), vol. 86, 1989, pages 5728 - 5732
SATO, INT. J. CL.IN. ONCOL., vol. 8, 2003, pages 200 - 206
SATO, INT. J. CLIN. ONCOL., vol. 8, 2003, pages 200 - 206
SCATCHARD, ANN. NY ACAD. SCI., vol. 51, 1949, pages 660 - 672
SCHIER ET AL., GENE, vol. 169, 1995, pages 147 - 155
SHALABY ET AL., J. EXP. MED., vol. 175, 1992, pages 217 - 225
SHERIFF ET AL., NATURE STRUCT. BIOL., vol. 3, 1996, pages 733 - 736
SHIBATA M-A ET AL: "Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model", CANCER GENE THERAPY, NORWALK, CT, US, vol. 15, no. 12, 1 July 2008 (2008-07-01), pages 776 - 786, XP002583086, ISSN: 0929-1903, [retrieved on 20080725], DOI: DOI:10.1038/CGT.2008.43 *
SHIELDS ET AL., J. BIOL. CHEM., vol. 9, no. 2, 2001, pages 6591 - 6604
SHIELDS ET AL., L. BIOL. CHERN., vol. 9, no. 2, 2001, pages 6591 - 6604
SHIN HAINSWORTH Y ET AL: "VEGF-C mediates cyclic pressure-induced endothelial cell proliferation.", PHYSIOLOGICAL GENOMICS, vol. 11, January 2003 (2003-01-01), pages 245 - 251, XP007915765, ISSN: 1094-8341 *
SHINKAI ET AL., JBIOL CHERN, vol. 273, 1998, pages 31283 - 31288
SIDHU ET AL., J. MOL. BIOL., vol. 338, no. 2, 2004, pages 299 - 310
SIMS ET AL., J. IMMUNOL., vol. 151, 1993, pages 2296
SKERRA ET AL., CURR. OPINION IN IMMUNOL., vol. 5, 1993, pages 256
SKOBE ET AL., NAT MED, vol. 7, 2001, pages 192 - 198
SKOBE ET AL., NATURE MEDICINE, vol. 7, 2001, pages 192 - 198
STACKER ET AL., FASEB J, vol. 16, 2002, pages 922 - 934
STACKER ET AL., NAT REV CANCER, vol. 2, 2002, pages 573 - 583
STELLA ET AL.: "Directed Drug Delivery", 1985, HUMANA PRESS, article "Prodrugs: A Chemical Approach to Targeted Drug Delivery", pages: 247 - 267
STINCHCOMB ET AL., NATURE, vol. 282, 1979, pages 39
STREIT; DETMAR, ONCOGENE, vol. 22, 2003, pages 3172 - 3179
SU ET AL., CANCER CELL, vol. 9, 2006, pages 209 - 223
SURESH ET AL., METHODS IN ENZYMOLOGY, vol. 121, 1986, pages 210
SYRIGOS; EPENETOS, ANTICANCER RESEARCH, vol. 19, 1999, pages 605 - 614
THORPE ET AL.: "Monoclonal Antibodies '84: Biological And Clinical Applications", 1985, article "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review", pages: 475 - 506
TIMOSHENKO A V ET AL: "Migration-promoting role of VEGF-C and VEGF-C binding receptors in human breast cancer cells", BRITISH JOURNAL OF CANCER, NATURE PUBLISHING GROUP, LONDON, GB, vol. 97, no. 8, 1 October 2007 (2007-10-01), pages 1090 - 1098, XP002478226, ISSN: 0007-0920, [retrieved on 20071002], DOI: DOI:10.1038/SJ.BJC.6603993 *
TOMLINSON ET AL., J. MOL. BIOL., vol. 227, 1992, pages 776 - 798
TONINI ET AL., ONCOGENE, vol. 22, 2003, pages 6549 - 6556
TONINI, ONCOGENE, vol. 22, 2003, pages 6549 - 6556
TRAUNECKER ET AL., EMBO.J., vol. 10, 1991, pages 3655
TREND BIOCHEM SCI, vol. 26, 2001, pages 230 - 235
TRENDS BIOTECHNOL, vol. 21, 2003, pages 484 - 490
TUTT ET AL., J. IMMUNOL., vol. 147, 1991, pages 60
UEDA MASATSUGU ET AL: "Vascular endothelial growth factor-C expression and invasive phenotype in ovarian carcinomas.", CLINICAL CANCER RESEARCH : AN OFFICIAL JOURNAL OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH 1 MAY 2005 LNKD- PUBMED:15867217, vol. 11, no. 9, 1 May 2005 (2005-05-01), pages 3225 - 3232, XP007915766, ISSN: 1078-0432 *
URDAL ET AL., J. BIOL. CHEM., vol. 263, 1988, pages 2870 - 2877
URLAUB ET AL., PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216
V. T. DEVITA ET AL.,: "Cancer: Principles and Practice of Oncology", 1993, LIPPINCOTT COMPANY
VASWANI; HAMILTON, ANN. ALLERGY, ASTHMA & IMMUNOL., vol. 1, 1998, pages 105 - 115
VERHOEYEN ET AL., SCIENCE, vol. 239, 1988, pages 1534 - 1536
VITETTA ET AL., SCIENCE, vol. 238, 1987, pages 1098
VOLLMERS; BRANDLEIN, HISTOLOGY AND HISTOPATHOLOGY, vol. 20, no. 3, 2005, pages 927 - 937
VOLLRNERS; BRANDLEIN, METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY, vol. 27, no. 3, 2005, pages 185 - 91
WALTENBERGER ET AL., J. BIOL CHEM, vol. 269, 1994, pages 26988 - 26995
WARREN ET AL., J. CLIN. INVEST., vol. 95, 1995, pages 1789 - 1797
WATERHOUSE ET AL., NUCL. ACIDS RES., vol. 21, 1993, pages 2265 - 2266
WEIDNER ET AL., N. ENGL. J. MED, vol. 324, 1991, pages 1 - 6
WELLS; LOWMAN, CURR. OPIN. STRUCT. BIOL., vol. 3, 1992, pages 355 - 362
WILLIAMS; WINTER, EUR. J. IMMUNOL., vol. 23, 1993, pages 1456 - 1461
WILMAN: "Prodrugs in Cancer Chemotherapy", BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 14, 1986, pages 375 - 382
WINTE ET AL., ANN. REV. IMMUNOL., vol. 12, 1994, pages 433 - 455
WINTER ET AL., ANN. REV. IMMUNOL., vol. 12, 1994, pages 433 - 455
WISEMAN ET AL., BLOOD, vol. 99, no. 12, 2002, pages 4336 - 42
WISEMAN ET AL., EUR. JOUR. NUCL. MED., vol. 27, no. 7, 2000, pages 766 - 77
WITZIG ET AL., J. CLIN. ONCOL., vol. 20, no. 10, 2002, pages 2453 - 63
WITZIG ET AL., J. CLIN. ONCOL., vol. 20, no. 15, 2002, pages 3262 - 69
WOYKE ET AL., ANTIMICROB. AGENTS AND CHEMOTHER., vol. 45, no. 12, 2001, pages 3580 - 3584
WRIGHT ET AL., TIBTECH, vol. 15, 1997, pages 26 - 32
WU ET AL., NATURE BIOTECHNOLOGY, vol. 23, no. 9, 2005, pages 1137 - 1146
XU ET AL., IMMUNITY, vol. 13, 2000, pages 37 - 45
YAMANE-OHNUKI ET AL., BIOTECH. BIOENG., vol. 87, 2004, pages 614
YANIV, NATURE, vol. 297, 1982, pages 17 - 18
YAZAKI; WU: "Methods in Molecular Biology", vol. 248, 2003, HUMANA PRESS, pages: 255 - 268
YELTON ET AL., J. IMMUNOL., vol. 155, 1995, pages 1994 - 2004
ZAPATA ET AL., PROTEIN ENG, vol. 8, no. 10, 1995, pages 1057 - 1062
ZAPATA, PROTEIN ENG., vol. 8, no. 10, 1995, pages 1057 - 1062
ZHU ET AL., PROTEIN SCIENCE, vol. 6, 1997, pages 781 - 788

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014064203A1 (fr) 2012-10-26 2014-05-01 INSERM (Institut National de la Santé et de la Recherche Médicale) Antagonistes lyve-1 utiles dans la prévention ou le traitement d'un état pathologique associé à la lymphangiogenèse
EP3272771A1 (fr) 2016-07-22 2018-01-24 Centre National De La Recherche Scientifique Traitement destiné à être utilisé pour prévenir les métastases chez un sujet exposé à un traitement anticancéreux induisant l'activation p38
WO2018015522A1 (fr) 2016-07-22 2018-01-25 Centre National De La Recherche Scientifique Traitement utilisé pour la prévention de la métastase chez un sujet exposé au traitement du cancer induisant l'activation de p38
WO2018189403A1 (fr) 2017-04-14 2018-10-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Méthodes et compositions pharmaceutiques destinées au traitement du cancer

Also Published As

Publication number Publication date
US20110143428A1 (en) 2011-06-16
US20120052566A9 (en) 2012-03-01
EP2509626B1 (fr) 2016-02-10
US9102720B2 (en) 2015-08-11
AR078377A1 (es) 2011-11-02
US20130344072A1 (en) 2013-12-26
JP2013513383A (ja) 2013-04-22
ES2565208T3 (es) 2016-04-01
TW201119674A (en) 2011-06-16
EP2509626A1 (fr) 2012-10-17
JP5818805B2 (ja) 2015-11-18
TWI505836B (zh) 2015-11-01
US8486397B2 (en) 2013-07-16

Similar Documents

Publication Publication Date Title
US9777059B2 (en) Anti-VEGF antibodies
US9102720B2 (en) Anti-VEGF-C antibodies and methods using same
US8404811B2 (en) Humanized anti-EGFL7 antibodies and methods using same
US9266948B2 (en) Anti-Bv8 antibodies and uses thereof
NZ572177A (en) Anti-dll4 antibodies and methods using same
AU2015200714B2 (en) Anti-VEGF antibodies
AU2013204108B2 (en) Anti-VEGF antibodies

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10757138

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2010757138

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2012543094

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE