WO2008090434A1 - Substituted n-bicyclicalkyl bicyclic carboxyamide compounds - Google Patents

Substituted n-bicyclicalkyl bicyclic carboxyamide compounds Download PDF

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WO2008090434A1
WO2008090434A1 PCT/IB2008/000125 IB2008000125W WO2008090434A1 WO 2008090434 A1 WO2008090434 A1 WO 2008090434A1 IB 2008000125 W IB2008000125 W IB 2008000125W WO 2008090434 A1 WO2008090434 A1 WO 2008090434A1
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pain
compound
formula
trifluoromethyl
reaction
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PCT/IB2008/000125
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French (fr)
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Koji Ando
Sachiko Mihara
Satoshi Magayama
Kuzanari Nakao
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Pfizer Japan Inc.
Pfizer Inc.
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Publication of WO2008090434A1 publication Critical patent/WO2008090434A1/en

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D401/00Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D215/00Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/12Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D215/14Radicals substituted by oxygen atoms
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D217/00Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems
    • C07D217/02Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with only hydrogen atoms or radicals containing only carbon and hydrogen atoms, directly attached to carbon atoms of the nitrogen-containing ring; Alkylene-bis-isoquinolines
    • C07D217/04Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with only hydrogen atoms or radicals containing only carbon and hydrogen atoms, directly attached to carbon atoms of the nitrogen-containing ring; Alkylene-bis-isoquinolines with hydrocarbon or substituted hydrocarbon radicals attached to the ring nitrogen atom

Definitions

  • This invention relates to novel substituted N-bicyclic alkyl bicyclic-carboxamide compounds and to their use in therapy. These compounds are particularly useful as antagonists of the VR1 (Type I Vanilloid) receptor, and are thus useful for the treatment of pain, neuralgia, neuropathies, nerve injury, burns, migraine, carpal tunnel syndrome, fibromyalgia, neuritis, sciatica, pelvic hypersensitivity, bladder disease, inflammation, or the like in mammals, especially humans.
  • the present invention also relates to a pharmaceutical composition comprising the above compounds.
  • the Vanilloid receptor 1 is a ligand gated non-selective cation channel. It is believed to be a member of the transient receptor potential super family. VR1 is recognized as a polymodal nociceptor that integrates multiple pain stimuli, e.g., noxious heat, protons, and vanilloids (European Journal of Physiology 451 :151-159, 2005). A major distribution of VR1 is in the sensory (A ⁇ - and C-) fibres, which are bipolar neurons having somata in sensory ganglia. The peripheral fibers of these neurons innervate the skin, the mucosal membranes, and almost all internal organs.
  • VR1 exists in bladder, kidney, brain, pancreas, and various kinds of organs.
  • WO2004014377 discloses a variety of 4-hydroxyquinoline derivatives as matrix metalloproteinase inhibitors.
  • WO2002030426 discloses a variety of naphthalenyl carboxamide derivatives and quinoline carboxamide derivatives as inhibitors of HIV integrase and inhibitors of HIV replication.
  • the present invention provides a compound of the following formula (I):
  • one of X 1 , X 2 and X 3 is N, and the others are CH;
  • X 4 is N and X 5 is CH, or X 4 and X 5 are CH, or X 4 is CH and X 5 is N;
  • R 1 is hydrogen or C 1 -C 4 alkyl
  • R 2 is hydrogen or halogen; or a pharmaceutically acceptable salt or solvate thereof.
  • halogen means fluoro, chloro, bromo or iodo, preferably fluoro or chloro.
  • (CrC 4 )alkyl mean straight or branched chain saturated radicals having the required number of carbon atoms, including methyl, ethyl, n-propyl, /so-propyl, n-butyl, /so-butyl, seconc/a/y-butyl and terf-butyl.
  • a preferred subgroup of compound of formula (I) includes compounds of formula (Ia)
  • R 1 is C 1 -C 4 alkyl
  • R 1 is hydrogen, methyl or ethyl; most preferably R 1 is hydrogen or methyl.
  • R 2 is hydrogen, fluoro or chloro.
  • X 1 is N, and X 2 and X 3 are both CH.
  • X 2 is N, and X 1 and X 3 are both CH.
  • X 3 is N, and X 1 and X 2 are both CH.
  • X 4 is N, and X 5 is CH.
  • X 5 is N, and X 4 is CH.(Quinolin- ⁇ -yl-) In a preferred embodiment, X 4 and X 5 are both CH.
  • the bicyclic moiety of the left hand (amine) side in formula (I) is isoquinolin-5-yl, isoquinolin-8-yl or quinolin-4-yl.
  • the bicyclic moiety on the right hand (carboxylic acid) side in formula (I) is quinolin-2-yl, quinolin-3-yl or naphthalene.
  • Preferred compounds of the invention include those in which each variable in formula (I) is selected from the preferred groups for each variable.
  • the compounds of formula (I), being VR1 antagonists, are potentially useful in the treatment of a range of disorders, particularly the treatment of pain, including chronic pain, acute pain, nociceptive pain, neuropathic pain, inflammatory pain, post herpetic neuralgia, neuropathies, neuralgia, diabetic neuropathy, HIV-related neuropathy, nerve injury, rheumatoid arthritic pain, osteoarthritic pain, burns, back pain, visceral pain, cancer pain, dental pain, headache, migraine, carpal tunnel syndrome, fibromyalgia, neuritis, sciatica, pelvic hypersensitivity, pelvic pain and menstrual pain; bladder disease, such as urinary incontinence, lower urinary tract symptoms, micturition disorder, renal colic and cystitis; inflammation, such as burns, rheumatoid arthritis and osteoarthritis; neurodegenerative disease, such as stroke, post stroke pain and multiple sclerosis; diseases of the respiratory tree that have a contribution to symptons or pathology
  • Physiological pain is an important protective mechanism designed to warn of danger from potentially injurious stimuli from the external environment.
  • the system operates through a specific set of primary sensory neurones and is activated by noxious stimuli via peripheral transducing mechanisms (see Millan, 1999, Prog. Neurobiol., 57, 1-164 for a review).
  • These sensory fibres are known as nociceptors and are characteristically small diameter axons with slow conduction velocities. Nociceptors encode the intensity, duration and quality of noxious stimulus and by virtue of their topographically organised projection to the spinal cord, the location of the stimulus.
  • nociceptive nerve fibres of which there are two main types, A-delta fibres (myelinated) and C fibres (non-myelinated).
  • A-delta fibres myelinated
  • C fibres non-myelinated.
  • the activity generated by nociceptor input is transferred, after complex processing in the dorsal horn, either directly, or via brain stem relay nuclei, to the ventrobasal thalamus and then on to the cortex, where the sensation of pain is generated.
  • Pain may generally be classified as acute or chronic. Acute pain begins suddenly and is short-lived (usually twelve weeks or less). It is usually associated with a specific cause such as a specific injury and is often sharp and severe. It is the kind of pain that can occur after specific injuries resulting from surgery, dental work, a strain or a sprain. Acute pain does not generally result in any persistent psychological response. In contrast, chronic pain is long-term pain, typically persisting for more than three months and leading to significant psychological and emotional problems. Common examples of chronic pain are neuropathic pain (e.g. painful diabetic neuropathy, postherpetic neuralgia), carpal tunnel syndrome, back pain, headache, cancer pain, arthritic pain and chronic post-surgical pain.
  • neuropathic pain e.g. painful diabetic neuropathy, postherpetic neuralgia
  • carpal tunnel syndrome e.g. painful diabetic neuropathy, postherpetic neuralgia
  • back pain e.g. painful diabetic neuropathy, postherpetic neuralgia
  • Clinical pain is present when discomfort and abnormal sensitivity feature among the patient's symptoms. Patients tend to be quite heterogeneous and may present with various pain symptoms. Such symptoms include: 1) spontaneous pain which may be dull, burning, or stabbing; 2) exaggerated pain responses to noxious stimuli (hyperalgesia); and 3) pain produced by normally innocuous stimuli (allodynia - Meyer et al., 1994, Textbook of Pain, 13-44). Although patients suffering from various forms of acute and chronic pain may have similar symptoms, the underlying mechanisms may be different and may, therefore, require different treatment strategies. Pain can also therefore be divided into a number of different subtypes according to differing pathophysiology, including nociceptive, inflammatory and neuropathic pain.
  • Nociceptive pain is induced by tissue injury or by intense stimuli with the potential to cause injury. Pain afferents are activated by transduction of stimuli by nociceptors at the site of injury and activate neurons in the spinal cord at the level of their termination. This is then relayed up the spinal tracts to the brain where pain is perceived (Meyer et al., 1994, Textbook of Pain, 13-44). The activation of nociceptors activates two types of afferent nerve fibres. Myelinated A-delta fibres transmit rapidly and are responsible for sharp and stabbing pain sensations, whilst unmyelinated C fibres transmit at a slower rate and convey a dull or aching pain.
  • Moderate to severe acute nociceptive pain is a prominent feature of pain from central nervous system trauma, strains/sprains, burns, myocardial infarction and acute pancreatitis, post-operative pain (pain following any type of surgical procedure), posttraumatic pain, renal colic, cancer pain and back pain.
  • Cancer pain may be chronic pain such as tumour related pain (e.g. bone pain, headache, facial pain or visceral pain) or pain associated with cancer therapy (e.g. postchemotherapy syndrome, chronic postsurgical pain syndrome or post radiation syndrome). Cancer pain may also occur in response to chemotherapy, immunotherapy, hormonal therapy or radiotherapy.
  • Back pain may be due to herniated or ruptured intervertebral discs or abnormalities of the lumber facet joints, sacroiliac joints, paraspinal muscles or the posterior longitudinal ligament. Back pain may resolve naturally but in some patients, where it lasts over 12 weeks, it becomes a chronic condition which can be particularly debilitating.
  • Neuropathic pain is currently defined as pain initiated or caused by a primary lesion or dysfunction in the nervous system. Nerve damage can be caused by trauma and disease and thus the term 'neuropathic pain' encompasses many disorders with diverse aetiologies. These include, but are not limited to, peripheral neuropathy, diabetic neuropathy, post herpetic neuralgia, trigeminal neuralgia, back pain, cancer neuropathy, HIV neuropathy, phantom limb pain, carpal tunnel syndrome, central post-stroke pain and pain associated with chronic alcoholism, hypothyroidism, uremia, multiple sclerosis, spinal cord injury, Parkinson's disease, epilepsy and vitamin deficiency. Neuropathic pain is pathological as it has no protective role.
  • neuropathic pain are difficult to treat, as they are often heterogeneous even between patients with the same disease (Woolf & Decosterd, 1999, Pain Supp., 6, S141 -S 147; Woolf and Mannion, 1999, Lancet, 353, 1959-1964). They include spontaneous pain, which can be continuous, and paroxysmal or abnormal evoked pain, such as hyperalgesia (increased sensitivity to a noxious stimulus) and allodynia (sensitivity to a normally innocuous stimulus).
  • the inflammatory process is a complex series of biochemical and cellular events, activated in response to tissue injury or the presence of foreign substances, which results in swelling and pain (Levine and Taiwo, 1994, Textbook of Pain, 45-56).
  • Arthritic pain is the most common inflammatory pain.
  • Rheumatoid disease is. one of the commonest chronic inflammatory conditions in developed countries and rheumatoid arthritis is a common cause of disability. The exact aetiology of rheumatoid arthritis is unknown, but current hypotheses suggest that both genetic and microbiological factors may be important (Grennan & Jayson, 1994, Textbook of Pain, 397-407).
  • Visceral pain is pain associated with the viscera, which encompass the organs of the abdominal cavity. These organs include the sex organs, spleen and part of the digestive system. Pain associated with the viscera can be divided into digestive visceral pain and non-digestive visceral pain.
  • Gl gastrointestinal
  • BFD functional bowel disorder
  • IBD inflammatory bowel disease
  • Gl disorders include a wide range of disease states that are currently only moderately controlled, including, in respect of FBD, gastro-esophageal reflux, dyspepsia, irritable bowel syndrome (IBS) and functional abdominal pain syndrome (FAPS), and, in respect of IBD, Crohn's disease, ileitis and ulcerative colitis, all of which regularly produce visceral pain.
  • Other types of visceral pain include the pain associated with dysmenorrhea, cystitis and pancreatitis and pelvic pain.
  • pain have multiple aetiologies and thus can be classified in more than one area, e.g. back pain and cancer pain have both nociceptive and neuropathic components.
  • Other types of pain include:
  • heart and vascular pain including pain caused by angina, myocardical infarction, mitral stenosis, pericarditis, Raynaud's phenomenon, scleredoma and skeletal muscle ischemia;
  • head pain such as migraine (including migraine with aura and migraine without aura), cluster headache, tension-type headache mixed headache and headache associated with vascular disorders; and
  • orofacial pain including dental pain, otic pain, burning mouth syndrome and temporomandibular myofascial pain.
  • Urinary incontinence (any condition in which there is an involuntary leakage of urine), includes stress urinary incontinence, urge urinary incontinence and mixed urinary incontinence, overactive bladder with associated urinary incontinence, enuresis, nocturnal enuresis, continuous urinary incontinence, and situational urinary incontinence such as incontinence during sexual intercourse.
  • Lower urinary tract symptoms comprise three groups of urinary symptoms, which may be defined as storage (irritative), voiding (obstructive) and post-micturition symptoms.
  • Storage symptoms comprise urgency, frequency, nocturia, urgency incontinence and stress incontinence, which can be associated with overactive bladder (OAB) and benign prostatic hyperplasia (BPH).
  • Voiding symptoms comprise hesitancy, poor flow, intermittency, straining and dysuria.
  • Post-micturition symptoms comprise terminal dribbling, post-void dribbling and a sense of incomplete emptying.
  • Over Active Bladder is defined as urgency, with or without urge incontinence, usually with frequency and nocturia [Abrams et al., Neurourology and Urodynamics 21 : 167-178 (2002)].
  • Prevalence of OAB in men and women is similar, with approximately 16% of the population of the USA suffering from the condition [Stewart et al, Prevalence of Overactive Bladder in the United States: Results from the NOBLE Program; Abstract Presented at the 2 nd International Consultation on Incontinence, July 2001 , Paris, France].
  • OAB includes OAB Wet and OAB Dry. The terms OAB Wet and OAB Dry describe OAB patients with or without urinary incontinence, respectively.
  • BPH is a chronically progressive disease that can lead to complications such as acute urinary retention, recurrent urinary tract infections, bladder stones and renal dysfunction.
  • the prevalence and average severity of LUTS associated with BPH in men increases with age.
  • BPH leads to an increase in prostate volume, creating urethral and bladder outflow obstruction as well as secondary changes in bladder function. The effects of this are manifested by both storage (irritative) and voiding (obstructive) symptoms.
  • the present invention provides a pharmaceutical composition including a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, together with a pharmaceutically acceptable excipient.
  • the composition is preferably useful for the treatment of the disease conditions defined above.
  • the present invention further provides a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use as a medicament.
  • the present invention further provides a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use in the treatment of the disease conditions defined above.
  • the present invention provides a method for the treatment of the disease conditions defined above in a mammal, preferably a human, which includes administering to said mammal a therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof.
  • the present invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament for the treatment of the disease conditions defined above.
  • the present invention provides a combination of a compound of the formula (I), or a pharmaceutically acceptable salt or. solvate thereof, and another pharmacologically active agent.
  • the compounds of the present invention may be prepared by a variety of processes well known for the preparation of compounds of this type, for example as shown in the following reaction Schemes.
  • a compound of formula (I) may be prepared according to scheme 1 below.
  • Step 1A an amide compound of formula (I) can be prepared by the coupling reaction of an amine compound of formula (II) with an acid compound of formula (III) in the presence or absence of a coupling reagent in an inert solvent.
  • Suitable coupling reagents are those typically used in peptide synthesis including, for example, diimides (e.g., DCC, EDC, 2-ethoxy-N-ethoxycarbonyl-1 ,2-dihydroquinoline, BEP, CDI, BOP, diethyl azodicarboxylate-triphenylphosphine, diethylcyanophosphate, diethylphosphorylazide, 2-chloro-1-methylpyridinium iodide, N, N'-carbonyldiimidazole , benzotriazole-1-yl diethyl phosphate, ethyl chloroformate or isobutyl chloroformate.
  • diimides e.g., DCC, EDC, 2-ethoxy-N-ethoxycarbonyl-1 ,2-dihydroquinoline, BEP, CDI, BOP, diethyl azodicarboxylate-triphenylphosphine, dieth
  • the reaction can be carried out in the presence of a base such as HOBt, N.N-diisopropylethylamine, N-methylmorpholine or triethylamine.
  • a base such as HOBt, N.N-diisopropylethylamine, N-methylmorpholine or triethylamine.
  • the amide compound of formula (I) can also be formed via the reaction of a compound of formula (II) with an acylhalide, which is obtained by the reaction of a compound of formula (III) with halogenating agents such as oxalylchloride, phosphorus oxychloride or thionyl chloride.
  • the reaction is normally and preferably effected in the presence of a solvent.
  • solvent there is no particular restriction on the nature of the solvent to be employed, provided that it has no adverse effect on the reaction or on the reagents involved and that it can dissolve the reagents, at least to some extent.
  • suitable solvents include; acetone; nitromethane; DMF; NMP; sulfolane; DMSO; 2-butanone; acetonitrile; halogenated hydrocarbons such as DCM, dichloroethane or chloroform; and ethers such as THF or 1 ,4-dioxane.
  • the reaction can take place over a wide range of temperatures, and the precise reaction temperature is not critical to the invention. The preferred reaction temperature will depend upon such factors as the nature of the solvent, and the starting material or reagent used.
  • reaction in general, we find it convenient to carry out the reaction at a temperature of from -20 0 C to 100 0 C, more preferably from about 0 0 C to 60 0 C.
  • the time required for the reaction can also vary widely, depending on many factors, notably the reaction temperature and the nature of the reagents and solvent employed. However, provided that the reaction is effected under the preferred conditions outlined above, a period of 5 minutes to 1 week, more preferably 30 minutes to 24 hours, will usually suffice.
  • a compound of formula (V) can be prepared by a coupling reaction of a compound of formula (IV) with 1-(vinyloxy)butane under basic conditions in the presence of a transition metal catalyst and additives in a solvent.
  • suitable solvents include: protic solvents such as water, alcohols such as MeOH or EtOH, and co-solvents of water or alcohols as protic solvents mixed with THF, 1 ,4-dioxane, DMF or acetonitrile.
  • This reaction can be carried out in the presence of a suitable catalyst.
  • a suitable catalyst There is likewise no particular restriction on the nature of the catalyst used, and any catalysts commonly used in reactions of this type can equally be used here.
  • Such catalysts include: tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, copper(O), copper(l) acetate, copper(l) bromide, copper(l) chloride, copper(l) iodide, copper(l) oxide, cop ⁇ er(ll) trifluoromethanesulfonat ⁇ , copper(ll) acetate, copper(ll) bromide, copper(ll) chloride, copper(ll) iodide, copper(ll) oxide, copper(ll) trifluoromethanesulfonate, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibe ⁇ zylideneacetone)palladium(0), tris(dibe ⁇ zylideneacetone)dipalladium(0) or [1 ,1 '-bis(diphenylphosphin
  • Preferred catalysts are tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibenzylide ⁇ eacetone)palladium(0), tris(dibe ⁇ zylideneacetone)dipalladium(0) or [1 ,1'-bis(diphenylphosphino)ferrocene]palladium(ll) dichloride.
  • This reaction can be carried out in the presence of a suitable additive agent.
  • additive agents include: triphenylphosphine, tri-te/t-butylphosphine,
  • reaction can be carried out in the presence of bases such as potassium carbonate, sodium carbonate or cesium carbonate.
  • bases such as potassium carbonate, sodium carbonate or cesium carbonate.
  • the reaction can be carried out at a temperature of from 0 0 C to 200 0 C, more preferably from 20 0 C to 120 0 C.
  • Reaction time is, in general, from 5 minutes to 48 hours, more preferably 30 minutes to 24 hours will usually suffice.
  • a compound of formula (VII) can be prepared by coupling reaction of a compound of formula (V) with an amine of formula (Vl) in the presence of a dehydration reagent and/or HCI-MeOH and/or Lewis Acid.
  • a preferred dehydration reagent includes sodium sulfate, magnesium sulfate, calcium sulfate or methylformate.
  • suitable solvents include: THF; 1 ,4-dioxane; DMF; acetonitrile; alcohols such as MeOH or EtOH; halogenated hydrocarbons such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; or acetic acid.
  • Reaction temperature is generally in the range of from 0 to 200 0 C, preferably in the range of from 100 0 C to 140 0 C.
  • Reaction time is, in general, from 1 minute to a day, preferably from 5 minutes to 1 hour. If necessary, microwave conditions may be applied to the reaction.
  • Step 2C In this step, a compound of formula (VIII) can be prepared by reduction of a compound of formula (VII). This reaction may be carried out in the presence of a suitable reducing agent such as diboran, boran-methyl sulfide complex, sodium borohydhde, lithium borohydride, sodium borohydride, or lithium aluminum hydride, in an inert solvent selected from THF and diethyl ether.
  • a suitable reducing agent such as diboran, boran-methyl sulfide complex, sodium borohydhde, lithium borohydride, sodium borohydride, or lithium aluminum hydride
  • Reaction temperature is generally in the range of from -100 to 250 0 C, preferably in the range of from 0 0 C to the reflux temperature, but if necessary, lower or higher temperatures can be employed.
  • Reaction time is, in general, from 1 minute to a day, preferably from 20 minutes to 5 hours, however shorter or longer reaction times, if necessary, can be employed.
  • the reduction may also be carried out under known hydrogenation conditions such as in the presence of a metal catalyst such as Raney nickel catalysts in the presence or absence of hydrazine, palladium catalysts or platinum catalysts under hydrogen atmosphere.
  • This reaction may be carried out in an inert solvent such as MeOH, EtOH, and THF in the presence or absence of hydrogen chloride. If necessary, this reduction may be carried out under pressure in the range from about 0.5 to 10 kg/cm 2 , preferably in the range from 1 to 6 kg/cm 2 . Examples of suitable solvents are similar to those mentioned in Step 2B.
  • Step 2D In this Step, a compound of the formula (II) can be prepared by deprotection and/or salt formation of a compound of formula (VIII) under acidic conditions in an inert solvent using the method described by D. Cogan el al (Journal of American Chemical Society, 1999, 121 , pp268-269).
  • Suitbale acids include, for example, but not limited to hydrogen chloride, hydrogen bromide, trifluoromethane sulfonic acid, acetic acid or p-toluenesulfonic acid.
  • the reaction may be also carried out under known hydrogenation conditions such as in the presence of a metal catalyst such as palladium-carbon catalyst or platinum catalysts under hydrogen atmosphere.
  • reaction may be carried out in an inert solvent such as MeOH, EtOH, and THF in the presence or absence of hydrogen chloride. If necessary, this reduction may be carried out under pressure in the range from about 0.5 to 10 kg/cm 2 , preferably in the range from 1 to 6 kg/cm 2 .
  • Reaction temperature is generally in the range of from -100 0 C to 250 0 C, preferably in the range of from 0 0 C to the reflux temperature, but if necessary, lower or higher temperatures can be employed.
  • Reaction time is, in general, from 1 minute to 2 days, preferably from 20 minutes to 24 hours.
  • a compound of formula (IX) may be prepared by reacting a compound of formula (IV) with carbon monoxide and an alcohol (e.g. MeOH or EtOH) in the presence of a catalyst and/or base in an inert solvent.
  • suitable catalysts include palladium reagents, such as palladium acetate or palladium dibenzylacetone.
  • Suitable bases include N,N-diisopropylethylamine, N-methylmorpholine or triethylamine.
  • this reaction may be carried out in the presence or absence of an additive such as 1 ,1'-bis(diphenylphosphino)ferrocene, triphenylphosphine or 1 ,3-bis-(diphenylphosphino)propane (DPPP).
  • the reaction is normally and preferably effected in the presence of a solvent. There is no particular restriction on the nature of the solvent to be employed, provided that it has no adverse effect on the reaction or on the reagents involved and that it can dissolve the reagents, at least to some extent.
  • Suitable solvents include: acetone; nitromethane; DMF; sulfolane; DMSO; NMP; 2-butanone; acetonitrile; halogenated hydrocarbons such as DCM, dichloroethane or chloroform; or ethers, such as THF or 1 ,4-dioxane.
  • the reaction can take place over a wide range of temperatures, and the precise reaction temperature is not critical to the invention. The preferred reaction temperature will depend upon such factors as the nature of the solvent, and the starting material or reagent used. However, in general, we find it convenient to carry out the reaction at a temperature of from -20 0 C to 150 0 C, more preferably from about 50 0 C to 80 0 C.
  • the time required for the reaction may also vary widely, depending on many factors, notably the reaction temperature and the nature of the reagents and solvent employed. However, provided that the reaction is effected under the preferred conditions outlined above, a period of from 30 minutes to 24 hours, more preferably from 1 hour to 10 hours, will usually suffice.
  • an acid compound may be prepared by hydrolysis of a compound of formula (IX) in a solvent.
  • the hydrolysis may be carried out by conventional procedures. In a typical procedure, the hydrolysis is carried out under basic conditions in the presence of water.
  • Suitable bases include, for example, sodium hydroxide, potassium hydroxide or lithium hydroxide.
  • Suitable solvents include, for example, alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol or ethylene gylcol; ethers such as THF, DME or 1 ,4-dioxane; amides such as DMF or hexamethylphosphorictriamide; or sulfoxides such as DMSO.
  • This reaction may be carried out at a temperature in the range from -20 to 100 0 C, usually from 20 0 C to 65 0 C for from 30 minutes to 24 hours, usually from 60 minutes to 10 hours.
  • the hydrolysis may also be carried out under acidic conditions, e.g. in the presence of hydrogen halides, such as hydrogen chloride and hydrogen bromide; sulfonic acids, such as p-toluenesulfonic acid and benzenesulfonic acid; pyridium p-toluenesulfonate; and carboxylic acid, such as acetic acid and trifluoroacetic acid.
  • Suitable solvents include, for example, alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol, and ethylene gylcol; ethers such as THF, DME and 1 ,4-dioxane; amides such as DMF and hexamethylphosphorictriamide; and sulfoxides such as DMSO.
  • This reaction may be carried out at a temperature in the range from -20 to 100 0 C, usually from 20 0 C to 65°C for from 30 minutes to 24 hours, usually from 60 minutes to 10 hours.
  • Step 3B-2 In this step, an amide compound of formula (X) can be prepared from the product of 3B-1 by the same procedure as described in Step 1.
  • Step 3C In this Step, a compound of formula (Xl) can be prepared by reaction of a compound of formula (X) with an organometallic reagent R 1 M. R 1 M can be prepared
  • R 1 M in which M represents MgZ, can be generated by stirring Mg and R 1 Z, dibromoethane and I 2 at a temperature in the range of from 30 to 80 0 C.
  • This reaction may be carried out in the presence of an organometallic reagent or a metal.
  • organometallic reagents include alkyllithiums such as n-butyllithium, sec-butyllithium or tert-butyllithium; aryllithiums such as phenyllithium or lithium naphthylide.
  • suitable metals include magnesium.
  • Preferred inert solvents include, for example, hydrocarbons, such as hexane; ethers, such as diethyl ether, diisopropyl ether, DME, THF or 1 ,4-dioxane; or mixtures thereof.
  • Reaction temperature is generally in the range of from -100 to 50 0 C, preferably in the range of from -100 0 C to room temperature.
  • Reaction time is, in general, from 1 minute to a day, preferably from 1 hour to 10 hours.
  • Step 3D In this step, a compound of formula (XII) can be prepared by coupling reaction of a compound of formula (Xl) with an amine of formula (Vl) by the method described in
  • Step 3E In this Step, a compound of formula (XIII) can be prepared from a compound of formula (XII) by the method described in Step 2C above.
  • Step 3F In this step, a compound of the formula (II) can be prepared from a compound of formula (XIII) by the method described in Step 2D above.
  • a compound of formula (III) may be prepared by the process illustrated in Scheme 4.
  • Step 4A In this Step, a compound of formula (XV) can be prepared by N-substituted acrylation of a compound of formula (XIV) with dialkyl alkoxy methylenemalonate in a reaction inert solvent or without solvent.
  • suitable solvents include alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol, and ethylene glycol; and ethers such as THF, DME, and 1 ,4-dioxa ⁇ e.
  • the reaction can be carried out at a temperature in the range from 50°C to 150°C for from 30 minutes to 24 hours, usually from 60 minutes to 3 hours.
  • Step 4B In this Step, a compound of formula (XVI) can be prepared by thermal cyclization of a compound of formula (XV) in a reaction inert solvent.
  • suitable solvents include ethers such as phenyl ether. This reaction can be carried out at a temperature in the range from 200 to 300 0 C for from 30 minutes to 24 hours, usually 250 0 C for from 30 minutes to 5 hours.
  • Step 4C a compound of formula (XVII) can be prepared by chlorination of a compound of formula (XVI).
  • the reaction is carried out under chlorination conditions with a chlorination reagent in a reaction inert solvent or without solvent.
  • suitable solvents include THF; 1 ,4-dioxane; DMF; acetonitrile; halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; and acetic acid.
  • suitable halogenating reagents include phosphorus oxyhalide such as phosphorus oxychloride; and phosphorus oxybromide.
  • the reaction can be carried out at a temperature of from 0 0 C to 200 0 C, more preferably from ambient temperature to 150 0 C. Reaction times are, in general, from 5 minutes to 48 hours, more preferably from 30 minutes to 6 hours will usually suffice.
  • Step 4D In this Step, a dehalogenated compound of formula (XVIII) can be prepared by hydrogenation of a compound of formula (XVII) in a solvent.
  • Hydrogenation reaction is carried out under, for example, known hydrogenolysis conditions in the presence of a metal catalyst under a hydrogen atmosphere or in the presence of hydrogen sources such as formic acid or ammonium formate in a reaction inert solvent. If desired, the reaction may be carried out under basic conditions, for example, in the presence of triethylamine.
  • Preferred reagents are selected from, for example, nickel catalysts such as Raney nickel, palladium-carbon, palladiumhydroxide-carbon, platinumoxide, platinum-carbon, ruthenium-carbon, rhodium-aluminumoxide and tris[triphenyphosphine] rhodiumchloride.
  • nickel catalysts such as Raney nickel, palladium-carbon, palladiumhydroxide-carbon, platinumoxide, platinum-carbon, ruthenium-carbon, rhodium-aluminumoxide and tris[triphenyphosphine] rhodiumchloride.
  • suitable reaction inert aqueous or non-aqueous organic solvents include alcohols, such as MeOH, EtOH; ethers, such as THF or 1 ,4-dioxane; acetone; dimethylformamide; halogenated hydrocarbons, such as DCM, dichloroethane or chloroform; and acetic acid; or mixtures
  • Reaction times are, in general, from 10 minutes to 48 hours, preferably from 30 minutes to 24 hours.
  • This reaction can be carried out under hydrogen atmosphere at a pressure ranging from 1 to 100 atom, preferably from 1 to 10 atm.
  • the preferred conditions are the use of 5 or 10% palladium-carbon at from 20 to 25 ° C for 1 to 24 hours under hydrogen atmosphere using a balloon.
  • Step 4E In this Step, an acid compound of formula (III) can be prepared by hydrolysis of a compound of formula (XVIII) in a solvent by the method as described in Step 3B-1.
  • a compound of formula (III) may be prepared by the process illustrated in Scheme 5.
  • Step 5A In this Step, a N-oxide compound of formula (XX) can be prepared by oxidation of a compound of formula (XIX) in a reaction inert solvent.
  • the oxidation reaction may be carried out in the absence or presence of an additive agent in a reaction inert solvent.
  • preferred oxidation reagents include meta-chloroperbenzoic acid (mCPBA), hydrogen peroxide and peracetic acid.
  • reaction inert solvents examples include halogenated hydrocarbons, such as methylene chloride, chloroform, carbon tetrachloride and dichloroethane; ethers, such as diethyl ether, diisopropyl ether, DME, THF and 1 ,4-dioxane; acetonitrile; acetic acid; and water; or mixtures thereof.
  • Reaction temperatures are generally in the range from 0 ° C to 250 ° C, more preferably in the range from 0 ° C to 100 ° C.
  • Reaction times are, in general, from 1 minute to 10 days, more preferably from 20 minutes to 6 hours. This reaction may be carried out in the presence of a suitable catalyst.
  • a cyano compound of formula (XXI) can be prepared by cyanation of a compound of formula (XX) in a reaction inert solvent.
  • preferred cyanation reagents include trimethylsilanecarbonitrile (TMSCN), the combination of trimethylchlorosilane and sodium cyanide, and the combination of acylating agents such as N,N-dimethylcarbamoyl chloride with trimethylsilanecarbonitrile (TMSCN).
  • a preferred cyanation reagent is trimethylsilanecarbonitrile (TMSCN) in the presence of a base such triethylamine in a reaction inert solvent.
  • TMSCN trimethylsilanecarbonitrile
  • a base such triethylamine
  • reaction inert solvents include halogenated hydrocarbons, such as methylene chloride, chloroform, carbon tetrachloride and dichloroethane; ethers, such as diethyl ether, DME, THF and 1 ,4-dioxane; acetonitrile; DMF; DMSO; or mixtures thereof.
  • Reaction temperatures are generally in the range from 0 0 C to 250 0 C, more preferably in the range from 0 ° C to 100 ° C.
  • Reaction times are, in general, from 1 minute to 10 days, more preferably from 20 minutes to 24 hours.
  • an acid compound of formula (III) can be prepared by hydrolysis of a cyano compound of formula (XXI) in a solvent.
  • the hydrolysis can be carried out by conventional procedures. In a typical procedure, the hydrolysis may be carried out under basic conditions, e.g. in the presence of sodium hydroxide, potassium hydroxide or lithium hydroxide.
  • suitable solvents include alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol, and ethylene gylcol; ethers such as THF, DME, and 1 ,4-dioxane; amides such as DMF and hexamethylphosphorictriamide; and sulfoxides such as DMSO.
  • Preferable solvents are MeOH, EtOH, propanol, THF, DME, 1 ,4-dioxane, DMF and DMSO. This reaction can be carried out at a temperature in the range from -20 to 150°C, usually from 20°C to 100°C for 30 minutes to 24 hours, usually 60 minutes to 10 hours.
  • a compound of formula (XXIII) can be prepared by O-dealkylation of a compound of formula (XXII).
  • the reaction is carried out under O-dealkylation conditions with a dealkylating reagent in a reaction inert solvent or without solvent.
  • suitable solvents include halogenated hydrocarbons, such as dichloromethane, 1 ,2-dichloroethane, chloroform or carbon tetrachloride.
  • suitable dealkylating reagents include boron halides, such as boron trichloride and boron tribromide.
  • the reaction can be carried out at a temperature of from O 0 C to 200 0 C, more preferably from ambient temperature to 60 0 C. Reaction times are, in general, from 5 minutes to 48 hours, more preferably 30 minutes to 6 hours will usually suffice.
  • a compound of formula (XXIV) can be prepared by trifluoromethane sulfonation reaction of a compound of formula (XXIII) using trifluoromethane sulfonic acid anhydrate under basic conditions in an inert solvent.
  • a preferred base is selected from, for example, but not limited to: an alkali or alkaline earth metal hydroxide, alkoxide, carbonate, halide or hydride, such as sodium hydroxide, potassium hydroxide, sodium methoxide, sodium ethoxide, potassium terf-butoxide, sodium carbonate, potassium carbonate, potassium fluoride, sodium hydride or potassium hydride; or an amine such as triethylamine, tributylamine, diisopropylethylamine, 2,6-lutidine, pyridine or dimethylaminopyridine.
  • an alkali or alkaline earth metal hydroxide, alkoxide, carbonate, halide or hydride such as sodium hydroxide, potassium hydroxide, sodium methoxide, sodium ethoxide, potassium terf-butoxide, sodium carbonate, potassium carbonate, potassium fluoride, sodium hydride or potassium hydride
  • an amine such as triethylamine,
  • solvents examples include: toluene; xylene; DME; DMSO; THF; 1 ,4-dioxane; DMF; acetonitrile; halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; and diethylether.
  • Reaction temperatures are generally in the range of from -78 0 C to 200 0 C, preferably in the range of from 0 0 C to room temperature. Reaction times are, in general, from 1 minute to a day, preferably from 1 hour to 20 hours.
  • a compound of formula (XXV) can be prepared by palladium catalyzed reaction of a compound of formula (XXIV) using a suitable boron reagent in an inert solvent.
  • suitable solvents include: THF; 1 ,4-dioxane; DMF; acetonitrile; alcohols, such as methanol or ethanol; halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; and diethylether; in the presence or absence of an aqueous base such as aqueous KOH, NaOH, LiOH or K 2 CO 3 .
  • Suitable boron reagents include, for example, pinacolborane and bis(neopentyl glycolato)diboron.
  • suitable transition metal catalysts include: tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, copper(O), copper(l) acetate, copper(l) bromide, copper(l) chloride, copper(l) iodide, copper(l) oxide, copper(ll) trifluoromethanesulfonate, copper(ll) acetate, copper(ll) bromide, copper(ll) chloride, copper(ll) iodide, copper(ll) oxide, copper(ll) trifluoromethanesulfonate, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibenzyiideneacetone)palladium(0)
  • [1 ,1 '-bis(diphenylphosphino)ferrocene]palladium(ll) dichloride Preferred catalysts are tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibenzylideneacetone)palladium(0), tris(dibenzylideneacetone)dipalladium(0) and [1 ,1 '-bis(diphenylphosphino)ferrocene]palladium(ll) dichloride.
  • This reaction can be carried out in the presence of a suitable additive agent.
  • additive agents include: triphenylphosphine, tri-fe/t-butylphosphine,
  • reaction can be carried out at a temperature of from 0 0 C to 200 0 C, more preferably from 20 0 C to 120 0 C. Reaction times are, in general, from 5 minutes to 96 hours, more preferably from 30 minutes to 24 hours.
  • a compound of formula (XXVI) can be prepared by iodination of a compound of formula (XXV). This reaction was carried out according to the method described in
  • a trifluoromethyl compound of formula (XXVII) can be prepared from a compound of formula (XXVI) by trifluoromethylation using a trifluoromethyl anion source in an inert solvent.
  • suitable solvents include: THF; 1 ,4-dioxane; DMF; acetonitrile; and halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride.
  • suitable trifluoromethyl anion source reagents include for example, the combination of CF 2 CI 2 (Or CF 2 Br 2 )AZn or Cd/Cul,
  • reaction can be carried out at a temperature of from 0 0 C to 200 0 C, more preferably from
  • Reaction times are, in general, from 5 minutes to 96 hours, more preferably from 30 minutes to 24 hours.
  • a trifluoromethyl compound of formula (XXVIII) can be prepared from a compound of (XXVII) by alkali hydrolysis as disclosed in Step 3B-1.
  • VR1 antagonistic activity can be determined by the Ca 2+ imaging assay using human VR1 highly expressing cells.
  • the cells that highly express human VR1 receptors are obtainable from several different conventional methods. The one standard method is cloning from human Dorsal Root Ganglion (DRG) or kidney according to the methods such as described in the journal article; Nature, 389, pp816-824, 1997.
  • VR1 receptors highly expressing human keratinocytes are also known and published in the journal article (Biochemical and Biophysical Research Communications, 291 , pp124-129, 2002). In this article, human keratinocytes demonstrated VR1 mediated intracellular Ca 2+ increase by addition of capsaicin.
  • the cells that express human VR1 receptors were maintained in culture flask at 37 0 C in an environment containing 5% CO 2 until use in the assay.
  • the intracellular Ca 2+ imaging assay to determine VR1 antagonistic activities were done by following procedures.
  • the culture medium was removed from the flask and fura-2/AM fluorescent calcium indicator was added to the flask at a concentration of 5 ⁇ M in the medium.
  • the flask was placed in CO 2 incubator and incubated for 1 hour. Then the cells expressing the human VR1 receptors were detached from the flask follow by washing with phosphate buffer saline, PBS(-) and re-suspended in assay buffer.
  • the 80 ⁇ of aliquot of cell suspension (3.75x10 5 cells/ml) was added to the assay plate and the cells were spun down by centrifuge (950 rpm, 20 0 C, 3 minutes).
  • the capsaicin-induced changes in the intracellular calcium concentration were monitored using FDSS 6000 (Hamamatsu Photonics, Japan), a fluorometric imaging system.
  • FDSS 6000 Hamamatsu Photonics, Japan
  • the cell suspension in Krebs-Ringer HEPES (KRH) buffer (115 mM NaCI, 5.4 imM KCI, 1 mM MgSO 4 , 1.8 mM CaCI 2 , 11 mM D-Glucose, 25 mM HEPES, 0.96 mM Na 2 HPO 4 , pH 7.3) were pre-incubated with varying concentrations of the test compounds or KRH buffer (buffer control) for 15 minutes at room temperature under the dark condition. Then capsaicin solution, which gives 300 nM in assay mixture, was automatically added to the assay plate by the FDSS 6000. Acid stimulation assay
  • the Acid-induced changes in the intracellular calcium concentration were monitored using FDSS 6000 (Hamamatsu Photonics, Japan), a fluorometric imaging system.
  • the cell suspension in resting buffer (HBSS supplemented with 1OmM HEPES, pH 7.4) were pre-incubated with varying concentrations of the test compounds or resting buffer (buffer control) for 15 minutes at room temperature under the dark condition.
  • the cells were automatically added the stimulating solution (HBSS supplemented with MES, final assay buffer pH5.8) by the FDSS 6000.
  • the IC 5 O values of VR1 antagonists were determined from the half of the increase demonstrated by buffer control samples after acidic stimulation. Determination of antagonist activity
  • mice Male Sprague-Dawley rats (270-300 g; B.W., Charles River, Tsukuba, Japan) were used.
  • the chronic constriction injury (CCI) operation was performed according to the method described by Bennett and Xie (Bennett, G.J. and Xie, YK. Pain, 33:87-107, 1988). Briefly, animals were anesthetized with sodium pentobarbital (64.8 mg/kg, i.p.) and the left common sciatic nerve was exposed at the level of the middle of the thigh by blunt dissection through biceps femoris.
  • CCI chronic constriction injury
  • VFHs von Frey hairs
  • Caco-2 cells were grown on filter supports (Falcon HTS multiwell insert system) for 14 days. Culture medium was removed from both the apical and basolateral compartments and the monolayers were preincubated with pre-warmed 0.3 ml apical buffer and 1.0 ml basolateral buffer for 0.75 hour at 37°C in a shaker water bath at 50 cycles/min.
  • the apical buffer consisted of Hanks Balanced Salt Solution, 25 mM D-glucose monohydrate, 20 mM MES Biological Buffer, 1.25 mM CaCI 2 and 0.5 mM MgCI 2 (pH 6.5).
  • the basolateral buffer consisted of Hanks Balanced Salt Solution, 25 mM D-glucose monohydrate, 20 mM HEPES Biological Buffer, 1.25 mM CaCI 2 and 0.5 mM MgCI 2 (pH 7.4).
  • test compound solution (10 ⁇ M) in buffer was added to the apical compartment.
  • the inserts were moved to wells containing fresh basolateral buffer and incubated for 1 hr. Drug concentration in the buffer was measured by LC/MS analysis.
  • Flux rate (F, mass/time) was calculated from the slope of cumulative appearance of substrate on the receiver side and apparent permeability coefficient (P app ) was calculated from the following equation.
  • Receiver plate (MATRN PS50, Millipore) was filled with 300 ⁇ L of MES buffered HBSS (pH 6.5) containing 10 //M of the test compounds.
  • the acceptor plate was placed onto the donor plate to form a "sandwich" and was incubated at 3O 0 C for 2.5 hours. After the incubation period, acceptor, donor and initial donor solution (reference) were analyzed via LC-MS/MS. Data were reported as the effective permeability value in cm X 10 6 /sec and the membrane retention value.
  • Cell paste of HEK-293 cells expressing the HERG product can be suspended in 10-fold volume of 50 mM Tris buffer adjusted at pH 7.5 at 25 0 C with 2 M HCI containing 1 mM MgCI 2 , 10 mM KCI.
  • the cells were homogenized using a Polytron homogenizer (at the maximum power for 20 seconds) and centrifuged at 48,00Og for 20 minutes at 4°C. The pellet was resuspended, homogenized and centrifuged once more in the same manner. The resultant supernatant was discarded and the final pellet was resuspended (10-fold volume of 50 mM Tris buffer) and homogenized at the maximum power for 20 seconds.
  • the membrane homogenate was aliquoted and stored at -8O 0 C until use. An aliquot was used for protein concentration determination using a Protein Assay Rapid Kit and ARVO SX plate reader (Wallac). All the manipulation, stock solution and equipment were kept on ice at all time. For saturation assays, experiments were conducted in a total volume of 200 ⁇ . Saturation was determined by incubating 20 ⁇ of [ 3 H]-dofetilide and 160 ⁇ of membrane homogenates (20-30 ⁇ g protein per well) for 60 min at room temperature in the absence or presence of 10 ⁇ M dofetilide at final concentrations (20 //I) for total or nonspecific binding, respectively.
  • SPA Proximity Assay
  • HEK 293 cells which stably express the HERG potassium channel were used for electrophysiological study.
  • the methodology for stable transfection of this channel in HEK cells can be found elsewhere (Z.Zhou et al., 1998, Biophysical Journal, 74, pp230-241).
  • MEM Minimum Essential Medium
  • FCS Fetal Calf Serum
  • HERG currents were studied using standard patch clamp techniques in the whole-cell mode.
  • the cells were superfused with a standard external solution of the following composition (mM); NaCI, 130; KCI, 4; CaCI 2 , 2; MgCI 2 , 1 ; Glucose, 10; HEPES, 5; pH 7.4 with NaOH.
  • mM standard external solution of the following composition
  • Whole-cell recordings was made using a patch clamp amplifier and patch pipettes which have a resistance of 1-3MOhm when filled with the standard internal solution of the following composition (mM); KCI, 130; MgATP, 5; MgCI 2 , 1.0; HEPES, 10; EGTA 5, pH 7.2 with KOH.
  • the voltage protocol was applied to a cell continuously throughout the experiment every 4 seconds (0.25Hz). The amplitude of the peak current elicited around -4OmV during the ramp was measured. Once stable evoked current responses were obtained in the external solution, vehicle (0.5% DMSO in the standard external solution) was applied for 10-20 min by a peristalic pump.
  • the test compound of either 0.3, 1 , 3, 10//M was applied for a 10 min period.
  • the 10 min period included the time which supplying solution was passing through the tube from solution reservoir to the recording chamber via the pump. Exposing time of cells to the compound solution was more than 5min after the drug concentration in the chamber well reached the attempting concentration. There was a subsequent wash period of a 10-20min to assess reversibility. Finally, the cells were exposed to high dose of dofetilide (5DM), a specific IKr blocker, to evaluate the insensitive endogenous current.
  • 5DM dofetilide
  • This method essentially involves determining the percent inhibition of product formation from fluorescence probe at 3 ⁇ M of the each compound.
  • the assay is carried out as follows.
  • the compounds were pre-incubated with recombinant CYPs, 100 mM potassium phosphate buffer and fluorescence probe as substrate for 5min.
  • Reaction was started by adding a warmed NADPH generating system, which consist of 0.5 mM NADP (expect; for 2D6 0.03 mM), 10 mM MgCI 2 , 6.2 mM DL-lsocitric acid and 0.5 U/ml lsocitric Dehydrogenase (ICD).
  • the assay plate was incubated at 37°C (expect; for 1A2 and 3A4 at 30 0 C) and taking fluoresce reading every minutes over 20 to 30min.
  • V 0 rate of control reaction (no inhibitor)
  • Vj rate of reaction in the presence of compounds.
  • Test compounds (1 ⁇ M) were incubated with 1 mM MgCk, 1 nnM NADP+, 5 mM isocitric acid, 1 U/mL isocitric dehydrogenase and 0.8 mg/mL HLM(human liver microsomes) in 100 mM potassium phosphate buffer (pH 7.4) at 37°C on a number of 384-well plates. At several time points, a plate was removed from the incubator and the reaction was terminated with two incubation volumes of acetonitrile. The compound concentration in supernatant was measured by LC/MS/MS system.
  • Rats were trained to measure the WB once a week until 20 days post MIA-injection. Analgesic effects of compounds were measured at 21 days after the MIA injection. Before the compound administration, the "pre value" of WB deficit was measured. After the administration of compounds, attenuation of WB deficits was determined as analgesic effects.
  • CFA Complete Freund's adjuvant
  • CFA 300 ⁇ g of Mycobacterium Tuberculosis H37RA (Difco, Ml) in 100 ⁇ L of liquid paraffin (Wako, Osaka, Japan)
  • PWT paw withdrawal threshold
  • the animals were gently restrained, and steadily increasing pressure was applied to the dorsal surface of a hind paw via a plastic tip. The pressure required to elicit paw withdrawal was determined.
  • the test compound was administered in a volume of 0.5 mL per 100 g body weight. PWT were measured after 1 , 3 or 5 hours after drug administration.
  • Pharmaceutically acceptable salts of the compounds of formula (I) include the acid addition and base salts thereof.
  • Suitable acid addition salts are formed from acids which form non-toxic salts. Examples include acetate, aspartate, benzoate, besylate, bicarbonate/carbonate, bisulphate/sulphate, borate, camsylate, citrate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, hibenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, maleate, malonate, mesylate, methylsulphate, naphthylate, 2-napsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, saccharate, stearate, succinate, tartrate, tosylate and trifluoro
  • Suitable base salts are formed from bases which form non-toxic salts. Examples include the aluminum, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, potassium, sodium, tromethamine and zinc salts.
  • a pharmaceutically acceptable salt of a compound of formula (I) may be readily prepared by mixing together solutions of the compound of formula (I) and the desired acid or base, as appropriate.
  • the salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent.
  • the degree of ionization in the salt may vary from completely ionized to almost non-ionized.
  • the compounds of the invention may exist in both unsolvated and solvated forms.
  • 'solvate' is used herein to describe a molecular complex comprising the compound of the invention and one or more pharmaceutically acceptable solvent molecules, for example, EtOH.
  • solvent molecules for example, EtOH.
  • 'hydrate' is employed when said solvent is water.
  • complexes such as clathrates, drug-host inclusion complexes wherein, in contrast to the aforementioned solvates, the drug and host are present in stoichiometric or non-stoichiometric amounts.
  • complexes of the drug containing two or more organic and/or inorganic components which may be in stoichiometric or non-stoichiometric amounts.
  • the resulting complexes may be ionized, partially ionized, or non-ionized.
  • references to compounds of formula (I) include references to salts, solvates and complexes thereof and to solvates and complexes of salts thereof.
  • the compounds of the invention include compounds of formula (I) as hereinbefore defined, polymorphs, prodrugs, and isomers thereof (including optical, geometric and tautomeric isomers) as hereinafter defined and isotopically-labeled compounds of formula (I).
  • the invention includes all polymorphs of the compounds of formula (I) as hereinbefore defined.
  • 'prodrugs' of the compounds of formula (I) are so-called 'prodrugs' of the compounds of formula (I).
  • certain derivatives of compounds of formula (I) which may have little or no pharmacological activity themselves can, when administered into or onto the body, be converted into compounds of formula (I) having the desired activity, for example, by hydrolytic cleavage.
  • Such derivatives are referred to as 'prodrugs'.
  • Further information on the use "of prodrugs may be found in 'Pro-drugs as Novel Delivery Systems, Vol. 14, ACS Symposium Series (T Higuchi and W Stella) and 'Bioreversible Carriers in Drug Design', Pergamon Press, 1987 (ed. E B Roche, American Pharmaceutical Association).
  • Prodrugs in accordance with the invention can, for example, be produced by replacing appropriate functionalities present in the compounds of formula (I) with certain moieties known to those skilled in the art as 'pro-moieties' as described, for example, in "Design of Prodrugs” by H Bundgaard (Elsevier, 1985).
  • prodrugs in accordance with the invention include: (i) where the compound of formula (I) contains a carboxylic acid functionality (-COOH), an ester thereof, for example, replacement of the hydrogen with (C-i-C ⁇ Jalkyl; (ii) where the compound of formula (I) contains an alcohol functionality (-OH), an ether thereof, for example, replacement of the hydrogen with (Ci-C 6 )alkanoyloxymethyl; and (iii) where the compound of formula (I) contains a primary or secondary amino functionality (-NH 2 or -NHR where R is not H), an amide thereof, for example, replacement of one or both hydrogens with (d-C 10 )alkanoyl.
  • a carboxylic acid functionality -COOH
  • an ester thereof for example, replacement of the hydrogen with (C-i-C ⁇ Jalkyl
  • the compound of formula (I) contains an alcohol functionality (-OH), an ether thereof, for example, replacement of the hydrogen with (Ci-C 6 )alkano
  • the present invention includes all pharmaceutically acceptable isotopically-labelled compounds of formula (I) wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature.
  • isotopes suitable for inclusion in the compounds of the invention include isotopes of hydrogen, such as 2 H and 3 H, carbon, such as 11 C, 13 C and 14 C, chlorine, such as 36 CI, fluorine, such as 18 F, iodine, such as 123 I and 125 I, nitrogen, such as 13 N and 15 N, oxygen, such as 15 O, 17 O and 18 O, phosphorus, such as 32 P, and sulphur, such as 35 S.
  • isotopically-Iabelled compounds of formula (I), for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies.
  • the radioactive isotopes tritium, i.e. 3 H, and carbon-14, i.e. 14 C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection.
  • Substitution with heavier isotopes such as deuterium, i.e. 2 H may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.
  • Isotopically-labeled compounds of formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples and Preparations using an appropriate isotopically-labeled reagents in place of the non-labeled reagent previously employed.
  • solvates in accordance with the invention include those wherein the solvent of crystallization may be isotopically substituted, e.g. D 2 O, d 6 -acetone, d 6 -DMSO.
  • Compounds of the invention intended for pharmaceutical use may be administered as crystalline or amorphous products. They may be obtained, for example, as solid plugs, powders, or films by methods such as precipitation, crystallization, freeze drying, or spray drying, or evaporative drying. Microwave or radio frequency drying may be used for this purpose.
  • excipient is used herein to describe any ingredient other than the compound(s) of the invention. The choice of excipient will to a large extent depend on factors such as the particular mode of administration, the effect of the excipient on solubility and stability, and the nature of the dosage form.
  • compositions suitable for the delivery of compounds of the present invention and methods for their preparation will be readily apparent to those skilled in the art. Such compositions and methods for their preparation may be found, for example, in 'Remington's Pharmaceutical Sciences', 19th Edition (Mack Publishing Company, 1995).
  • the compounds of the invention may be administered orally.
  • Oral administration may involve swallowing, so that the compound enters the gastrointestinal tract, or buccal or sublingual administration may be employed by which the compound enters the blood stream directly from the mouth.
  • Formulations suitable for oral administration include solid formulations such as tablets, capsules containing particulates, liquids, or powders, lozenges (including liquid-filled), chews, multi- and nano-particulates, gels, solid solution, liposome, films (including muco-adhesive), ovules, sprays and liquid formulations.
  • Liquid formulations include suspensions, solutions, syrups and elixirs. Such formulations may be employed as fillers in soft or hard capsules and typically comprise a carrier, for example, water, EtOH, polyethylene glycol, propylene glycol, methylcellulose, or a suitable oil, and one or more emulsifying agents and/or suspending agents. Liquid formulations may also be prepared by the reconstitution of a solid, for example, from a sachet.
  • the compounds of the invention may also be used in fast-dissolving, fast-disintegrating dosage forms such as those described in Expert Opinion in Therapeutic Patents, 11 (6), 981-986 by Liang and Chen (2001).
  • the drug may make up from 1 wt% to 80 wt% of the dosage form, more typically from 5 wt% to 60 wt% of the dosage form.
  • tablets generally contain a disintegrant.
  • disintegrants include sodium starch glycolate, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, croscarmellose sodium, crospovidone, polyvinylpyrrolidone, methyl cellulose, microcrystalline cellulose, lower alkyl-substituted hydroxypropyl cellulose, starch, pregelatinised starch and sodium alginate.
  • the disintegrant will comprise from 1 wt% to 25 wt%, preferably from 5 wt% to 20 wt% of the dosage form.
  • Binders are generally used to impart cohesive qualities to a tablet formulation. Suitable binders include microcrystalline cellulose, gelatin, sugars, polyethylene glycol, natural and synthetic gums, polyvinylpyrrolidone, pregelatinised starch, hydroxypropyl cellulose and hydroxypropyl methylcellulose. Tablets may also contain diluents, such as lactose (monohydrate, spray-dried monohydrate, anhydrous and the like), mannitol, xylitol, dextrose, sucrose, sorbitol, microcrystalline cellulose, starch and dibasic calcium phosphate dihydrate.
  • lactose monohydrate, spray-dried monohydrate, anhydrous and the like
  • mannitol xylitol
  • dextrose sucrose
  • sorbitol microcrystalline cellulose
  • starch dibasic calcium phosphate dihydrate
  • Tablets may also optionally comprise surface active agents, such as sodium lauryl sulfate and polysorbate 80, and glidants such as silicon dioxide and talc.
  • surface active agents such as sodium lauryl sulfate and polysorbate 80
  • glidants such as silicon dioxide and talc.
  • surface active agents may comprise from 0.2 wt% to 5 wt% of the tablet, and glidants may comprise from 0.2 wt% to 1 wt% of the tablet.
  • Tablets also generally contain lubricants such as 1 magnesium stearate, calcium stearate, zinc stearate, sodium stearyl fumarate, and mixtures of magnesium stearate with sodium lauryl sulphate.
  • Lubricants generally comprise from 0.25 wt% to 10 wt%, preferably from 0.5 wt% to 3 wt% of the tablet.
  • ingredients include anti-oxidants, colorants, flavouring agents, preservatives and taste-masking agents.
  • Exemplary tablets contain up to about 80% drug, from about 10 wt% to about 90 wt% binder, from about 0 wt% to about 85 wt% diluent, from about 2 wt% to about 10 wt% disintegrant, and from about 0.25 wt% to about 10 wt% lubricant.
  • Tablet blends may be compressed directly or by roller to form tablets. Tablet blends or portions of blends may alternatively be wet-, dry-, or melt-granulated, melt congealed, or extruded before tabletting.
  • the final formulation may comprise one or more layers and may be coated or uncoated; it may even be encapsulated.
  • Solid formulations for oral administration may be formulated to be immediate and/or modified controlled release.
  • Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.
  • Suitable modified release formulations for the purposes of the invention are described in US Patent No. 6,106,864. Details of other suitable release technologies such as high energy dispersions and osmotic and coated particles are to be found in Verma et al, Pharmaceutical Technology On-line, 25(2), 1-14 (2001). The use of chewing gum to achieve controlled release is described in WO 00/35298. PARENTERAL ADMINISTRATION
  • the compounds of the invention may also be administered directly into the blood stream, into muscle, or into an internal organ.
  • Suitable means for parenteral administration include .intravenous, intraarterial, intraperitoneal, intrathecal, intraventricular, intraurethral, intrastemal, intracranial, intramuscular and subcutaneous.
  • Suitable devices for parenteral administration include needle (including microneedle) injectors, needle-free injectors and infusion techniques.
  • Parenteral formulations are typically aqueous solutions which may contain excipients such as salts, carbohydrates and buffering agents (preferably, to a pH of from 3 to 9), but, for some applications, they may be more suitably formulated as a sterile non-aqueous solution or as powdered a dried form to be used in conjunction with a suitable vehicle such as sterile, pyrogen-free water.
  • excipients such as salts, carbohydrates and buffering agents (preferably, to a pH of from 3 to 9)
  • a suitable vehicle such as sterile, pyrogen-free water.
  • parenteral formulations under sterile conditions may readily be accomplished using standard pharmaceutical techniques well known to those skilled in the art.
  • solubility of compounds of formula (I) used in the preparation of parenteral solutions may be increased by the use of appropriate formulation techniques, such as the incorporation of solubility-enhancing agents.
  • Formulations for use with needle-free injection administration comprise a compound of the invention in powdered form in conjunction with a suitable vehicle such as sterile, pyrogen-free water.
  • Formulations for parenteral administration may be formulated to be immediate and/or modified controlled release.
  • Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.
  • compounds of the invention may be formulated as a solid, semi-solid, or thixotropic liquid for administration as an implanted depot providing modified release of the active compound. Examples of such formulations include drug-coated stents and PGLA microspheres.
  • the compounds of the invention may also be administered topically to the skin or mucosa, that is, dermally or transdermally.
  • Typical formulations for this purpose include gels, hydrogels, lotions, solutions, creams, ointments, dusting powders, dressings, foams, films, skin patches, wafers, implants, sponges, fibres, bandages and microemulsions. Liposomes may also be used.
  • Typical carriers include alcohol, water, mineral oil, liquid petrolatum, white petrolatum, glycerin, polyethylene glycol and propylene glycol. Penetration enhancers may be incorporated - see, for example, J Pharm Sci, 88 (10), 955-958 by Finnin and Morgan (October 1999).
  • topical administration include delivery by electroporation, iontophoresis, phonophoresis, sonophoresis and microneedle or needle-free (e.g. PowderjectTM, BiojectTM, etc.) injection.
  • Formulations for topical administration may be formulated to be immediate and/or modified controlled release.
  • Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.
  • the compounds of the invention can also be administered intranasally or by inhalation, typically in the form of a dry powder (either alone, as a mixture, for example, in a dry blend with lactose, or as a mixed component particle, for example, mixed with phospholipids, such as phosphatidylcholine) from a dry powder inhaler or as an aerosol spray from a pressurized container, pump, spray, atomiser (preferably an atomiser using electrohydrodynamics to produce a fine mist), or nebuliser, with or without the use of a suitable propellant, such as 1 ,1 ,1 ,2-tetrafluoroethane or
  • the powder may comprise a bioadhesive agent, for example, chitosan or cyclodextrin.
  • the pressurized container, pump, spray, atomizer, or nebuliser contains a solution or suspension of the compound(s) of the invention comprising, for example, EtOH, aqueous EtOH, or a suitable alternative agent for dispersing, solubilising, or extending release of the active, a propellant(s) as solvent and an optional surfactant, such as sorbitan trioleate, oleic acid, or an oligolactic acid.
  • the drug product Prior to use in a dry powder or suspension formulation, the drug product is micronised to a size suitable for delivery by inhalation (typically less than 5 microns). This may be achieved by any appropriate comminuting method, such as spiral jet milling, fluid bed jet milling, supercritical fluid processing to form nanoparticles, high pressure homogenisation, or spray drying.
  • comminuting method such as spiral jet milling, fluid bed jet milling, supercritical fluid processing to form nanoparticles, high pressure homogenisation, or spray drying.
  • Capsules made, for example, from gelatin or HPMC
  • blisters and cartridges for use in an inhaler or insufflator may be formulated to contain a powder mix of the compound of the invention, a suitable powder base such as lactose or starch and a performance modifier such as /-leucine, mannitol, or magnesium stearate.
  • the lactose may be anhydrous or in the form of the monohydrate, preferably the latter.
  • Other suitable excipients include dextran, glucose, maltose, sorbitol, xylitol, fructose, sucrose and trehalose.
  • a suitable solution formulation for use in an atomiser using electrohydrodynamics to produce a fine mist may contain from 1 ⁇ g to 20mg of the compound of the invention per actuation and the actuation volume may vary from 1 ⁇ l to 100 ⁇ l.
  • a typical formulation may comprise a compound of formula (I), propylene glycol, sterile water, EtOH and sodium chloride.
  • Alternative solvents which may be used instead of propylene glycol include glycerol and polyethylene glycol.
  • Suitable flavours such as menthol and levomenthol, or sweeteners, such as saccharin or saccharin sodium, may be added to those formulations of the invention intended for inhaled/intranasal administration.
  • Formulations for inhaled/intranasal administration may be formulated to be immediate and/or modified controlled release using, for example, poly(DL-lactic-coglycolic acid (PGLA).
  • Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.
  • the dosage unit is determined by means of a valve which delivers a metered amount.
  • Units in accordance with the invention are typically arranged to administer a metered dose or "puff" containing from 1 ⁇ g to 10mg of the compound of formula (I).
  • the overall daily dose will typically be in the range 1 ⁇ g to 10 mg which may be administered in a single dose or, more usually, as divided doses throughout the day.
  • the compounds of the invention may be administered rectally or vaginally, for example, in the form of a suppository, pessary, or enema.
  • Cocoa butter is a traditional suppository base, but various alternatives may be used as appropriate.
  • Formulations for rectal/vaginal administration may be formulated to be immediate and/or modified controlled release.
  • Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.
  • the compounds of the invention may be combined with soluble macromolecular entities, such as cyclodextrin and suitable derivatives thereof or polyethylene glycol-containing polymers, in order to improve their solubility, dissolution rate, taste-masking, bioavailability and/or stability for use in any of the aforementioned modes of administration.
  • soluble macromolecular entities such as cyclodextrin and suitable derivatives thereof or polyethylene glycol-containing polymers
  • Drug-cyclodextrin complexes are found to be generally useful for most dosage forms and administration routes. Both inclusion and non-inclusion complexes may be used.
  • the cyclodextrin may be used as an auxiliary additive, i.e. as a carrier, diluent, or solubiliser. Most commonly used for these purposes are alpha-, beta- and gamma-cyclodextrins, examples of which may be found in International Patent Applications Nos. WO 91/11172, WO 94/02518 and WO 98/55148. DOSAGE
  • the total daily dose of the compounds of the invention is typically in the range 0.1 mg to 3000 mg, preferably from 1 mg to 500mg, depending, of course, on the mode of administration.
  • oral administration may require a total daily dose of from 0.1 mg to 3000 mg, preferably from 1mg to 500mg, while an intravenous dose may only require from 0.1 mg to 1000 mg, preferably from 0.1 mg to 300mg.
  • the total daily dose may be administered in single or divided doses.
  • These dosages are based on an average human subject having a weight of about 65kg to 70kg. The physician will readily be able to determine doses for subjects whose weight falls outside this range, such as infants and the elderly.
  • references herein to "treatment” include references to curative, palliative and prophylactic treatment.
  • a VR1 antagonist may be usefully combined with another pharmacologically active compound, or with two or more other pharmacologically active compounds, particularly in the treatment of pain.
  • a VR1 antagonist particularly a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, as defined above, may be administered simultaneously, sequentially or separately in combination with one or more agents selected from:
  • an opioid analgesic e.g. morphine, heroin, hydromorphone, oxymorphone, levorphanol, levallorphan, methadone, meperidine, fentanyl, cocaine, codeine, dihydrocodeine, oxycodone, hydrocodone, propoxyphene, nalmefene, nalorphine, naloxone, naltrexone, buprenorphine, butorphanol, nalbuphine or pentazocine;
  • NSAID nonsteroidal antiinflammatory drug
  • NSAID nonsteroidal antiinflammatory drug
  • diclofenac diflusinal, etodolac
  • fenbufen fenoprofen
  • flufenisal flurbiprofen
  • ibuprofen indomethacin
  • ketoprofen ketorolac
  • meclofenamic acid mefenamic acid
  • meloxicam nabumetone, naproxen, nimesulide, nitroflurbiprofen, olsalazine, oxaprozin, phenylbutazone, piroxicam, sulfasalazine, sulindac, tolmetin or zomepirac
  • NSAID nonsteroidal antiinflammatory drug
  • a barbiturate sedative e.g. amobarbital, aprobarbital, butabarbital, butabital, mephobarbital, metharbital, methohexital, pentobarbital, phenobartital, secobarbital, talbutal, theamylal or thiopental;
  • a benzodiazepine having a sedative action e.g. chlordiazepoxide, clorazepate, diazepam, flurazepam, lorazepam, oxazepam, temazepam or triazolam;
  • an Hi antagonist having a sedative action e.g. diphenhydramine, pyrilamine, promethazine, chlorpheniramine or chlorcyclizine; • a sedative such as glutethimide, meprobamate, methaqualone or dichloralphenazone;
  • a skeletal muscle relaxant e.g. baclofen, carisoprodol, chlorzoxazone, cyclobenzaprine, methocarbamol or orphrenadine;
  • an NMDA receptor antagonist e.g. dextromethorphan ((+)-3-hydroxy-N-methylmorphinan) or its metabolite dextrorphan ((+)-3-hydroxy-N-methylmorphinan), ketamine, memantine, pyrroloquinoline quinine, cis-4-(phosphonomethyl)-2-piperidinecarboxylic acid, budipine, EN-3231 (MorphiDex®, a combination formulation of morphine and dextromethorphan), topiramate, neramexane or perzinfotel including an NR2B antagonist, e.g.
  • an NMDA receptor antagonist e.g. dextromethorphan ((+)-3-hydroxy-N-methylmorphinan) or its metabolite dextrorphan ((+)-3-hydroxy-N-methylmorphinan), ketamine, memantine, pyrroloquinoline quinine, cis-4-(phosphonomethyl)-2
  • an alpha-adrenergic e.g. doxazosin, tamsulosin, clonidine, guanfacine, dexmetatomidine, modafinil, or 4-amino-6,7-dimethoxy-2-(5-methane-sulfonamido-1 ,2,3,4-tetrahydroisoquinol-2- yl)-5-(2-pyridyl) quinazoline;
  • a tricyclic antidepressant e.g. desipramine, imipramine, amitriptyline or nortriptyline;
  • an anticonvulsant e.g. carbamazepine, lamotrigine, topiratmate or valproate;
  • a tachykinin (NK) antagonist particularly an NK-3, NK-2 or NK-1 antagonist, e.g. ( ⁇ R,9R)-7-[3,5-bis(trifluoromethyl)benzyl]-8,9,10,11-tetrahydro-9-methyl-5-(4-met hylphenyl)-7H-[1 ,4]diazocino[2, 1 -g][1 ,7]-naphthyridine-6-13-dione (TAK-637), 5-[[(2R,3S)-2-[(1 R)-1-[3,5-bis(trifluoromethyl)phenyl]ethoxy-3-(4-fluorophenyl)-4- morpholinyl]-methyl]-1 ,2-dihydro-3H-1 ,2,4-triazol-3-one (MK-869), aprepitant, lanepitant, dapitant or 3-[[2-methoxy-5-(trifluorometh
  • a muscarinic antagonist e.g oxybutynin, tolterodine, propiverine, tropsium chloride, darifenacin, solifenacin, temiverine and ipratropium;
  • COX-2 selective inhibitor e.g. celecoxib, rofecoxib, parecoxib, valdecoxib, deracoxib, etoricoxib, or lumiracoxib;
  • a coal-tar analgesic in particular paracetamol
  • a neuroleptic such as droperidol, chlorpromazine, haloperidol, perphenazine, thioridazine, mesoridazine, trifluoperazine, fluphenazine, clozapine, olanzapine, risperidone, ziprasidone, quetiapine, sertindole, aripiprazole, sonepiprazole, blonanserin, iloperidone, perospirone, raclopride, zotepine, bifeprunox, asenapine, lurasidone, amisulpride, balaperidone, palindore, eplivanserin, osanetant, rimonabant, meclinertant, Miraxion® or sarizotan;
  • a vanilloid receptor agonist e.g. resinferatoxin
  • antagonist e.g. capsazepine
  • a beta-adrenergic such as propranolol
  • a corticosteroid such as dexamethasone
  • a 5-HT receptor agonist or antagonist particularly a 5-HT-
  • a 5-HT 2A receptor antagonist such as R(+)-alpha-(2,3-dimethoxy-phenyl)-1-[2-(4-fluorophenylethyl)]-4-piperidinemetha nol (MDL-100907);
  • a cholinergic (nicotinic) analgesic such as ispronicline (TC-1734), (E)-N-methyl-4-(3-pyridinyl)-3-buten-1 -amine (RJR-2403), (R)-5-(2-azetidinylmethoxy)-2-chloropyridine (ABT-594) or nicotine;
  • a PDEV inhibitor such as 5-[2-ethoxy-5-(4-methyl-1 -piperazinyl-sulphonyl)phenyl]-1 -methyl-3-n-propyl-1 ,6- dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one (sildenafil), (6R,12aR)-2,3,6,7,12,12a-hexahydro-2-methyl-6-(3,4-methylenedioxyphenyl)-pyr azino[2',1':6,1]-pyrido[3,4-b]indole-1 ,4-dione (IC-351 or tadalafil), 2-[2-ethoxy-5-(4-ethyl-piperazin-1-yl-1-sulphonyl)-phenyl]-5-methyl-7-propyl-3H-i midazo[5,1 -f][1 ,2,4]triazin-4-one (vardenafil), 5-
  • an alpha-2-delta ligand such as gabapentin, pregabalin, 3-methylgabapentin, (1a,3a,5a)(3-amino-methyl-bicyclo[3.2.0]hept-3-yl)-acetic acid, (3S,5R)-3-aminomethyl-5-methyl-heptanoic acid, (3S,5R)-3-amino-5-methyl-heptanoic acid, (3S,5R)-3-amino-5-methyl-octanoic acid, (2S,4S)-4-(3-chlorophenoxy)proline, (2S,4S)-4-(3-fluorobenzyl)-proline, [(1 R,5R,6S)-6-(aminonnethyl)bicyclo[3.2.0]hept-6-yl]acetic acid, 3-(1 -aminomethyl-cyclohexylmethyl)-4H-[1 ,2,4]oxadiazol-5-one,
  • mGluRI metabotropic glutamate subtype 1 receptor
  • a serotonin reuptake inhibitor such as sertraline, sertraline metabolite demethylsertraline, fluoxetine, norfluoxetine (fluoxetine desmethyl metabolite), fluvoxamine, paroxetine, citalopram, citalopram metabolite desmethylcitalopram, escitalopram, d,l-fenfluramine, femoxetine, ifoxetine, cyanodothiepin, litoxetine, dapoxetine, nefazodone, cericlamine and trazodone;
  • a noradrenaline (norepinephrine) reuptake inhibitor such as maprotiline, lofepramine, mirtazepine, oxaprotiline, fezolamine, tomoxetine, mianserin, buproprion, buproprion metabolite hydroxybuproprion, nomifensine and viloxazine (Vivalan®), especially a selective noradrenaline reuptake inhibitor such as reboxetine, in particular (S,S)-reboxetine;
  • a dual serotonin-noradrenaline reuptake inhibitor such as venlafaxine, venlafaxine metabolite O-desmethylvenlafaxine, clomipramine, clomipramine metabolite desmethylclomipramine, duloxetine, milnacipran and imipramine;
  • an inducible nitric oxide synthase (iNOS) inhibitor such as S-[2-[(1-iminoethyl)amino]ethyl]-L-homocysteine, S-[2-[(1-iminoethyl)-amino]ethyl]-4,4-dioxo-L-cysteine,
  • an acetylcholinesterase inhibitor such as donepezil
  • a prostaglandin E 2 subtype 4 (EP4) antagonist such as ⁇ /-[( ⁇ 2-[4-(2-ethyI-4,6-dimethyl-1 H-imidazo[4,5-c]pyridin-1-yl)phenyl]ethyI ⁇ amino)- carbonyl]-4-methylbenzenesulfonamide or 4-[(1 S)-1-( ⁇ [5-chloro-2-(3-fluorophenoxy)pyridin-3-yl]carbonyl ⁇ amino)ethyl]benzoi c acid;
  • a leukotriene B4 antagonist such as 1-(3-biphenyl-4-ylmethyl-4-hydroxy-chroman-7-yl)-cyclopentanecarboxylic acid (CP-105696), 5-[2-(2-Carboxyethyl)-3-[6-(4-methoxyphenyl)-5E- hexenyl]oxyphenoxy]-valeric acid (ONO-4057) or DPC-11870,
  • a 5-lipoxygenase inhibitor such as zileuton, 6-[(3-fluoro-5-[4-methoxy-3,4,5,6-tetrahydro-2H-pyran-4-yl])phenoxy-methyl]-1-m ethyl-2-quinoIone (ZD-2138), or 2,3,5-trimethyl-6-(3-pyridylmethyl),1 ,4-benzoquinone (CV-6504);
  • a sodium channel blocker such as lidocaine
  • a 5-HT3 antagonist such as ondansetron
  • pharmaceutically acceptable salts and solvates thereof • a 5-HT3 antagonist, such as ondansetron; and the pharmaceutically acceptable salts and solvates thereof.
  • kits suitable for coadministration of the compositions.
  • the kit of the invention comprises two or more separate pharmaceutical compositions, at least one of which contains a compound of formula (I) in accordance with the invention, and means for separately retaining said compositions, such as a container, divided bottle, or divided foil packet.
  • An example of such a kit is the familiar blister pack used for the packaging of tablets, capsules and the like.
  • the kit of the invention is particularly suitable for administering different dosage forms, for example, oral and parenteral, for administering the separate compositions at different dosage intervals, or for titrating the separate compositions against one another.
  • the kit typically comprises directions for administration and may be provided with a so-called memory aid. Examples
  • Flash column chromatography was carried out using Merck silica gel 60 (230-400 mesh ASTM) or Fuji Silysia amino bounded silica (Chromatorex, 30-50 uM) or Biotage amino bounded silica (35-75 Dm, KP-NH) or Biotage silica (32-63 Dm, KP-SiI).
  • the purification using HPLC was perfomed by the following apparatus and conditions.
  • Apparatus UV-trigger preparative HPLC system, Waters (Column: XTerra MS C18, 5 urn, 19 x 50 mm or 30 x 50 mm), Detector: UV 254 nm Conditions : CH 3 CN/0.05% HCOOH aqueous solution or CH 3 CN/0.01% NH 3 aqueous solution; 20ml/min (19 x 50 mm) or 40ml/min (30 x 50 mm) at ambient temperature. Microwave apparatus used in the reaction was Emrys optimizer (Personal chemistry). Optical rotation was measured by P-1020 (Jasco). Low-resolution mass spectral data (El) were obtained on a Integrity (Waters) mass spectrometer.
  • ESI mass spectral data
  • ZMD Micromass mass spectrometer
  • IR spectra were measured by a Shimazu infrared spectrometer (IR-470). Chemical symbols have their usual meanings; bp (boiling point), mp (melting point), L (liter(s)), ml (milliliter(s)), g (gram(s)), mg (milligram(s)), mol (moles), mmol (millimoles), eq. (equivalent(s)), quant, (quantitative yield), sat. (saturated), aq (aqua).
  • "Me” means methyl
  • Et means ethyl.
  • 1-Quinolin-4-ylethanamine dihydrochloride can be prepared by the method described in Journal of the Chemical Society, Perkin Transactions 2, (1999), (11), pp 2415-2418.
  • Amine 2 1-lsoquinolin-5-ylmethanamine hydrochloride
  • Step 5-B 1-(6,8-difluoroquinolin-4-v0methanamine dihvdrochloride
  • 1 H NMR (270 MHz, CDCI 3 ) ⁇ 4.51-4.62 (m, 2H), 7.78-7.97 (3H, m), 8.85-9.05 (3H, m).
  • Amine 6 1-quinolin-4-ylmethanamine
  • 1 -Quinolin-4-ylmethanamine can be prepared by the method described in Yakugaku
  • Carboxylic acid 1 6-(trifluoromethvO-2-naphthoic acid
  • Carboxylic acid 1 was synthesized following the procedure described in Synthesis (2005), (5), pp 791-797.
  • Carboxvlic acid 2 6-(trifluoromethv0quinoline-2-carboxvlic acid
  • Carboxylic acid 3 7-(trifluoromethyl)quinoline-3-carboxylic acid
  • Carboxylic acid 3 was synthesized following the procedure described in Journal of
  • Carboxylic acid 4 6-Fluoro-7-(trifluoromethyl)quinoline-3-carboxylic acid
  • Step 4-1 Ethyl 6-fluoro-4-hvdroxy-7-(trifluoromethyl)quinoline-3-carboxylate
  • Step 4-2 Ethyl 4-chloro-6-fluoro-7-(trifluoromethvDquinoline-3-carboxylate
  • Step 4-3 Ethyl 6-fluoro-7-(trifluoromethyl)quinoline-3-carboxylate A mixture of the product of Step 4-2 (3.38 g, 10.5 mmol), 5 % palladium on activated carbon (338 mg), triethylamine (2.93 mL, 21.0 mmol) and ethanol (50 mL) was hydrogenated (1 atm, balloon) at room temperature for 90 minutes. The reaction mixture was filtered over a pad of celite, and the filtrate was evaporated.
  • Step 4-4 ⁇ -Fluoro ⁇ -ftrifluoromethvDquinoline-S-carboxylic acid
  • ethanol 4 ml
  • 2N aqueous sodium hydroxide 10.2 mL, 20.5 mmol
  • the reaction mixture was neutralized to pH 5-6 by 2N aqueous hydrochloride.
  • the formed precipitate was collected and washed with water to furnish the title compound (2.52 g, 95 %) as a white solid.
  • Carboxylic acid 5 ⁇ -Chloro ⁇ -ftrifluoromethvO ⁇ uinoline-S-carboxylic acid
  • Step 5-1 Ethyl 6-chloro-4-hvdroxy-7-(trifluoromethv ⁇ uinoline-3-carboxylate
  • Step 5-2 Ethyl 4-bromo-6-chloro-7-(trifluoromethyl)quinoline-3-carboxylate
  • Step 5-3 Ethyl 6-chloro-7-(trifluoromethyl)quinoline-3-carboxylate
  • step 5-2 A mixture of the product of step 5-2 (2.15 g, 5.63 mmol), 5 % palladium on activated carbon (215 mg), triethylamine (1.57 ml_, 11.30 mmol) and ethanol (56 ml_) was hydrogenated (1 atm, balloon) at room temperature for 55 minutes.
  • the reaction mixture was filtered over a pad of celite, and the filtrate was evaporated.
  • the residue was chromatographed on a column of silica gel, eluting with ethyl acetate-hexane (1 :10 to
  • Step 5-4 e-Chloro ⁇ -ftrifluoromethvDquinoline-S-carboxylic acid
  • Examples 2 to 11 were prepared according to the process disclosed in example 1 , using the appropriate starting material.
  • Example 2 ⁇ /-(Quinolin-4-ylmethyl)-6-(trifluoromethyl)-2-naphthamide
  • Example 2 was prepared from Amine 1 and Carboxylic Acid 1.
  • Example 3 was prepared from Amine 2 and Carboxylic Acid 3.
  • Example 4 was prepared from Amine 1 and Carboxylic Acid 3.
  • Example 5 was prepared from Amine 3 and Carboxylic Acid 3
  • HPLC condition Waters (Column: Xterra PrepMS C18, 3.5um, 4.6x50mm), Detector: photodiodearray (210- 400 nm) Conditions: water / MeOH / 1% NH 3 aqueous solution;
  • Example 6 was prepared from Amine 2 and Carboxylic Acid 2
  • Example 7 was prepared from Amine 1 and Carboxylic Acid 2
  • Example 8 was prepared from Amine 2 and Carboxylic Acid 4
  • Example 9 was prepared from Amine 2 and Carboxylic Acid 5.
  • Example 10 was prepared from Amine 6 and Carboxylic Acid 4.
  • Example 11 was prepared from Amine 6 and Carboxylic Acid 5.

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Abstract

This invention provides compounds of the formula (I): and their use for the treatment of disease conditions caused by overactivation of the VR1 receptor such as pain, or the like in mammal. The invention also provides pharmaceutical compositions comprising the above compounds.

Description

SUBSTITUTED N-BICYCLICALKYL BICYCUC CARBOXYAMIDE COMPOUNDS Technical Field
This invention relates to novel substituted N-bicyclic alkyl bicyclic-carboxamide compounds and to their use in therapy. These compounds are particularly useful as antagonists of the VR1 (Type I Vanilloid) receptor, and are thus useful for the treatment of pain, neuralgia, neuropathies, nerve injury, burns, migraine, carpal tunnel syndrome, fibromyalgia, neuritis, sciatica, pelvic hypersensitivity, bladder disease, inflammation, or the like in mammals, especially humans. The present invention also relates to a pharmaceutical composition comprising the above compounds. Background Art
The Vanilloid receptor 1 (VR1) is a ligand gated non-selective cation channel. It is believed to be a member of the transient receptor potential super family. VR1 is recognized as a polymodal nociceptor that integrates multiple pain stimuli, e.g., noxious heat, protons, and vanilloids (European Journal of Physiology 451 :151-159, 2005). A major distribution of VR1 is in the sensory (Aδ- and C-) fibres, which are bipolar neurons having somata in sensory ganglia. The peripheral fibers of these neurons innervate the skin, the mucosal membranes, and almost all internal organs. It is also recognized that VR1 exists in bladder, kidney, brain, pancreas, and various kinds of organs. A body of studies using VR1 agonists, e.g., capsaicin or resiniferatoxin, have suggested that VR1 positive nerves are thought to participate in a variety of physiological responses, including nociception (Clinical Therapeutics. 13(3): 338-395, 1991 , Journal of Pharmacology and Experimental Therapeutics 314:410-421 , 2005, and Neuroscience Letter 388: 75-80, 2005). Based on both the tissue distribution and the roles of VR1 , VR1 antagonists would have good therapeutic potential.
WO2004014377 discloses a variety of 4-hydroxyquinoline derivatives as matrix metalloproteinase inhibitors. WO2002030426 discloses a variety of naphthalenyl carboxamide derivatives and quinoline carboxamide derivatives as inhibitors of HIV integrase and inhibitors of HIV replication.
It would be desirable if there were provided improved VR1 selective antagonists with enhanced binding activity with the VR1 receptor by systemic administration and with a good half-life. Other potential advantages include less toxicity, good absorption, good solubility, low protein binding affinity, less drug-drug interaction, a reduced inhibitory activity at HERG channel, reduced QT prolongation and good metabolic stability. Brief Disclosure of the Invention It has now been found that certain substituted carboxamide derivatives are potent VR1 antagonists with analgesic activity by systemic administration.
The present invention provides a compound of the following formula (I):
Figure imgf000003_0001
wherein, one of X1, X2 and X3 is N, and the others are CH;
X4 is N and X5 is CH, or X4 and X5 are CH, or X4 is CH and X5 is N;
R1 is hydrogen or C1-C4 alkyl;
R2 is hydrogen or halogen; or a pharmaceutically acceptable salt or solvate thereof.
Detailed Description of the Invention
As used herein, the term "halogen" means fluoro, chloro, bromo or iodo, preferably fluoro or chloro.
As used herein, the terms "(CrC4)alkyl" mean straight or branched chain saturated radicals having the required number of carbon atoms, including methyl, ethyl, n-propyl, /so-propyl, n-butyl, /so-butyl, seconc/a/y-butyl and terf-butyl.
A preferred subgroup of compound of formula (I) includes compounds of formula (Ia)
Figure imgf000003_0002
wherein R1 is C1-C4 alkyl
In a preferred embodiment, R1 is hydrogen, methyl or ethyl; most preferably R1 is hydrogen or methyl.
In a preferred embodiment, R2 is hydrogen, fluoro or chloro. In a preferred embodiment, X1 is N, and X2 and X3 are both CH. (lsoquinolin-8-yl)
In a preferred embodiment, X2 is N, and X1 and X3 are both CH. (lsoquinolin-5-yl) In a preferred embodiment, X3 is N, and X1 and X2 are both CH. (Quinolin-4-yl) In a preferred embodiment, X4 is N, and X5 is CH. (Quinolin-2-yl) In a preferred embodiment, X5 is N, and X4 is CH.(Quinolin-β-yl-) In a preferred embodiment, X4 and X5 are both CH. (Naphthyl-)
Preferably the bicyclic moiety of the left hand (amine) side in formula (I) is isoquinolin-5-yl, isoquinolin-8-yl or quinolin-4-yl. Preferably the bicyclic moiety on the right hand (carboxylic acid) side in formula (I) is quinolin-2-yl, quinolin-3-yl or naphthalene.
Preferred compounds of the invention include those in which each variable in formula (I) is selected from the preferred groups for each variable.
Specific preferred compounds of the invention are those listed in the Examples section below and the pharmaceutically acceptable salts and solvates thereof.
The compounds of formula (I), being VR1 antagonists, are potentially useful in the treatment of a range of disorders, particularly the treatment of pain, including chronic pain, acute pain, nociceptive pain, neuropathic pain, inflammatory pain, post herpetic neuralgia, neuropathies, neuralgia, diabetic neuropathy, HIV-related neuropathy, nerve injury, rheumatoid arthritic pain, osteoarthritic pain, burns, back pain, visceral pain, cancer pain, dental pain, headache, migraine, carpal tunnel syndrome, fibromyalgia, neuritis, sciatica, pelvic hypersensitivity, pelvic pain and menstrual pain; bladder disease, such as urinary incontinence, lower urinary tract symptoms, micturition disorder, renal colic and cystitis; inflammation, such as burns, rheumatoid arthritis and osteoarthritis; neurodegenerative disease, such as stroke, post stroke pain and multiple sclerosis; diseases of the respiratory tree that have a contribution to symptons or pathology arising from the sensory afferent nervous system, such as cough, bronchoconstriction, irritation, inflammation and other pathways in diseases of the lower airway such as asthma and COPD as well as those of the upper airway, such as allergic rhinitis and chronic sinusitis; gastrointestinal disorders, such as gastroesophageal reflux disease (GERD), dysphagia, ulcer, irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), colitis and Crohn's disease; ischemia, such as cerebrovascular ischemia and acute cerebral ischemia; emesis, such as cancer chemotherapy-induced emesis; diabetes and obesity; or the like in mammals, especially humans. The treatment of pain, particularly inflammatory pain, is a preferred use. Physiological pain is an important protective mechanism designed to warn of danger from potentially injurious stimuli from the external environment. The system operates through a specific set of primary sensory neurones and is activated by noxious stimuli via peripheral transducing mechanisms (see Millan, 1999, Prog. Neurobiol., 57, 1-164 for a review). These sensory fibres are known as nociceptors and are characteristically small diameter axons with slow conduction velocities. Nociceptors encode the intensity, duration and quality of noxious stimulus and by virtue of their topographically organised projection to the spinal cord, the location of the stimulus. The nociceptors are found on nociceptive nerve fibres of which there are two main types, A-delta fibres (myelinated) and C fibres (non-myelinated). The activity generated by nociceptor input is transferred, after complex processing in the dorsal horn, either directly, or via brain stem relay nuclei, to the ventrobasal thalamus and then on to the cortex, where the sensation of pain is generated.
Pain may generally be classified as acute or chronic. Acute pain begins suddenly and is short-lived (usually twelve weeks or less). It is usually associated with a specific cause such as a specific injury and is often sharp and severe. It is the kind of pain that can occur after specific injuries resulting from surgery, dental work, a strain or a sprain. Acute pain does not generally result in any persistent psychological response. In contrast, chronic pain is long-term pain, typically persisting for more than three months and leading to significant psychological and emotional problems. Common examples of chronic pain are neuropathic pain (e.g. painful diabetic neuropathy, postherpetic neuralgia), carpal tunnel syndrome, back pain, headache, cancer pain, arthritic pain and chronic post-surgical pain.
When a substantial injury occurs to body tissue, via disease or trauma, the characteristics of nociceptor activation are altered and there is sensitisation in the periphery, locally around the injury and centrally where the nociceptors terminate. These effects lead to a heightened sensation of pain. In acute pain these mechanisms can be useful, in promoting protective behaviours which may better enable repair processes to take place. The normal expectation would be that sensitivity returns to normal once the injury has healed. However, in many chronic pain states, the hypersensitivity far outlasts the healing process and is often due to nervous system injury. This injury often leads to abnormalities in sensory nerve fibres associated with maladaptation and aberrant activity (Woolf & Salter, 2000, Science, 288, 1765-1768).
Clinical pain is present when discomfort and abnormal sensitivity feature among the patient's symptoms. Patients tend to be quite heterogeneous and may present with various pain symptoms. Such symptoms include: 1) spontaneous pain which may be dull, burning, or stabbing; 2) exaggerated pain responses to noxious stimuli (hyperalgesia); and 3) pain produced by normally innocuous stimuli (allodynia - Meyer et al., 1994, Textbook of Pain, 13-44). Although patients suffering from various forms of acute and chronic pain may have similar symptoms, the underlying mechanisms may be different and may, therefore, require different treatment strategies. Pain can also therefore be divided into a number of different subtypes according to differing pathophysiology, including nociceptive, inflammatory and neuropathic pain.
Nociceptive pain is induced by tissue injury or by intense stimuli with the potential to cause injury. Pain afferents are activated by transduction of stimuli by nociceptors at the site of injury and activate neurons in the spinal cord at the level of their termination. This is then relayed up the spinal tracts to the brain where pain is perceived (Meyer et al., 1994, Textbook of Pain, 13-44). The activation of nociceptors activates two types of afferent nerve fibres. Myelinated A-delta fibres transmit rapidly and are responsible for sharp and stabbing pain sensations, whilst unmyelinated C fibres transmit at a slower rate and convey a dull or aching pain. Moderate to severe acute nociceptive pain is a prominent feature of pain from central nervous system trauma, strains/sprains, burns, myocardial infarction and acute pancreatitis, post-operative pain (pain following any type of surgical procedure), posttraumatic pain, renal colic, cancer pain and back pain. Cancer pain may be chronic pain such as tumour related pain (e.g. bone pain, headache, facial pain or visceral pain) or pain associated with cancer therapy (e.g. postchemotherapy syndrome, chronic postsurgical pain syndrome or post radiation syndrome). Cancer pain may also occur in response to chemotherapy, immunotherapy, hormonal therapy or radiotherapy. Back pain may be due to herniated or ruptured intervertebral discs or abnormalities of the lumber facet joints, sacroiliac joints, paraspinal muscles or the posterior longitudinal ligament. Back pain may resolve naturally but in some patients, where it lasts over 12 weeks, it becomes a chronic condition which can be particularly debilitating.
Neuropathic pain is currently defined as pain initiated or caused by a primary lesion or dysfunction in the nervous system. Nerve damage can be caused by trauma and disease and thus the term 'neuropathic pain' encompasses many disorders with diverse aetiologies. These include, but are not limited to, peripheral neuropathy, diabetic neuropathy, post herpetic neuralgia, trigeminal neuralgia, back pain, cancer neuropathy, HIV neuropathy, phantom limb pain, carpal tunnel syndrome, central post-stroke pain and pain associated with chronic alcoholism, hypothyroidism, uremia, multiple sclerosis, spinal cord injury, Parkinson's disease, epilepsy and vitamin deficiency. Neuropathic pain is pathological as it has no protective role. It is often present well after the original cause has dissipated, commonly lasting for years, significantly decreasing a patient's quality of life (Woolf and Mannion, 1999, Lancet, 353, 1959-1964). The symptoms of neuropathic pain are difficult to treat, as they are often heterogeneous even between patients with the same disease (Woolf & Decosterd, 1999, Pain Supp., 6, S141 -S 147; Woolf and Mannion, 1999, Lancet, 353, 1959-1964). They include spontaneous pain, which can be continuous, and paroxysmal or abnormal evoked pain, such as hyperalgesia (increased sensitivity to a noxious stimulus) and allodynia (sensitivity to a normally innocuous stimulus).
The inflammatory process is a complex series of biochemical and cellular events, activated in response to tissue injury or the presence of foreign substances, which results in swelling and pain (Levine and Taiwo, 1994, Textbook of Pain, 45-56). Arthritic pain is the most common inflammatory pain. Rheumatoid disease is. one of the commonest chronic inflammatory conditions in developed countries and rheumatoid arthritis is a common cause of disability. The exact aetiology of rheumatoid arthritis is unknown, but current hypotheses suggest that both genetic and microbiological factors may be important (Grennan & Jayson, 1994, Textbook of Pain, 397-407). It has been estimated that almost 16 million Americans have symptomatic osteoarthritis (OA) or degenerative joint disease, most of whom are over 60 years of age, and this is expected to increase to 40 million as the age of the population increases, making this a public health problem of enormous magnitude (Houge & Mersfelder, 2002, Ann Pharmacother., 36, 679-686; McCarthy et al., 1994, Textbook of Pain, 387-395). Most patients with osteoarthritis seek medical attention because of the associated pain. Arthritis has a significant impact on psychosocial and physical function and is known to be the leading cause of disability in later life. Ankylosing spondylitis is also a rheumatic disease that causes arthritis of the spine and sacroiliac joints. It varies from intermittent episodes of back pain that occur throughout life to a severe chronic disease that attacks the spine, peripheral joints and other body organs.
Another type of inflammatory pain is visceral pain which includes pain associated with inflammatory bowel disease (IBD). Visceral pain is pain associated with the viscera, which encompass the organs of the abdominal cavity. These organs include the sex organs, spleen and part of the digestive system. Pain associated with the viscera can be divided into digestive visceral pain and non-digestive visceral pain. Commonly encountered gastrointestinal (Gl) disorders that cause pain includes functional bowel disorder (FBD) and inflammatory bowel disease (IBD). These Gl disorders include a wide range of disease states that are currently only moderately controlled, including, in respect of FBD, gastro-esophageal reflux, dyspepsia, irritable bowel syndrome (IBS) and functional abdominal pain syndrome (FAPS), and, in respect of IBD, Crohn's disease, ileitis and ulcerative colitis, all of which regularly produce visceral pain. Other types of visceral pain include the pain associated with dysmenorrhea, cystitis and pancreatitis and pelvic pain.
It should be noted that some types of pain have multiple aetiologies and thus can be classified in more than one area, e.g. back pain and cancer pain have both nociceptive and neuropathic components. Other types of pain include:
• pain resulting from musculoskeletal disorders, including myalgia, fibromyalgia, spondylitis, sero-negative (non-rheumatoid) arthropathies, non-articular rheumatism, dystrophinopathy, glycogenosis, polymyositis and pyomyositis;
• heart and vascular pain, including pain caused by angina, myocardical infarction, mitral stenosis, pericarditis, Raynaud's phenomenon, scleredoma and skeletal muscle ischemia;
• head pain, such as migraine (including migraine with aura and migraine without aura), cluster headache, tension-type headache mixed headache and headache associated with vascular disorders; and
• orofacial pain, including dental pain, otic pain, burning mouth syndrome and temporomandibular myofascial pain.
Urinary incontinence (any condition in which there is an involuntary leakage of urine), includes stress urinary incontinence, urge urinary incontinence and mixed urinary incontinence, overactive bladder with associated urinary incontinence, enuresis, nocturnal enuresis, continuous urinary incontinence, and situational urinary incontinence such as incontinence during sexual intercourse.
Lower urinary tract symptoms comprise three groups of urinary symptoms, which may be defined as storage (irritative), voiding (obstructive) and post-micturition symptoms. Storage symptoms comprise urgency, frequency, nocturia, urgency incontinence and stress incontinence, which can be associated with overactive bladder (OAB) and benign prostatic hyperplasia (BPH). Voiding symptoms comprise hesitancy, poor flow, intermittency, straining and dysuria. Post-micturition symptoms comprise terminal dribbling, post-void dribbling and a sense of incomplete emptying.
Over Active Bladder (OAB) is defined as urgency, with or without urge incontinence, usually with frequency and nocturia [Abrams et al., Neurourology and Urodynamics 21 : 167-178 (2002)]. Prevalence of OAB in men and women is similar, with approximately 16% of the population of the USA suffering from the condition [Stewart et al, Prevalence of Overactive Bladder in the United States: Results from the NOBLE Program; Abstract Presented at the 2nd International Consultation on Incontinence, July 2001 , Paris, France]. OAB includes OAB Wet and OAB Dry. The terms OAB Wet and OAB Dry describe OAB patients with or without urinary incontinence, respectively. Until recently, the cardinal symptom of OAB was believed to be urinary incontinence. However, with the advent of the new terms this is clearly not meaningful for the large number of sufferers who are not incontinent (i.e. OAB Dry patients). Thus, a recent study from Liberman et al ['Health Related Quality of Life Among Adults with Symptoms of Overactive Bladder: Results From A US Community-Based Survey'; Urology 57(6), 1044-1050, 2001] examined the impact of all OAB symptoms on the quality of life of a community-based sample of the US population. This study demonstrated that individuals suffering from OAB without any demonstrable loss of urine have an impaired quality of life when compared with controls.
BPH is a chronically progressive disease that can lead to complications such as acute urinary retention, recurrent urinary tract infections, bladder stones and renal dysfunction. The prevalence and average severity of LUTS associated with BPH in men increases with age. BPH leads to an increase in prostate volume, creating urethral and bladder outflow obstruction as well as secondary changes in bladder function. The effects of this are manifested by both storage (irritative) and voiding (obstructive) symptoms.
The present invention provides a pharmaceutical composition including a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, together with a pharmaceutically acceptable excipient. The composition is preferably useful for the treatment of the disease conditions defined above.
The present invention further provides a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use as a medicament.
The present invention further provides a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, for use in the treatment of the disease conditions defined above.
Further, the present invention provides a method for the treatment of the disease conditions defined above in a mammal, preferably a human, which includes administering to said mammal a therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof.
Yet further, the present invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament for the treatment of the disease conditions defined above.
Yet further, the present invention provides a combination of a compound of the formula (I), or a pharmaceutically acceptable salt or. solvate thereof, and another pharmacologically active agent.
In this specification, especially in "General Synthesis" and "Examples", the following abbreviations can be used: BEP 2-bromo-1-ethylpyridinium tetrafluoroborate
BOP benzotriazol-1 -yloxy-tris(dimethylamino)phosphonium hexafluorophosphate
CDI 2-chloro-1 ,3-dimethylimidazolinium chloride
DCC dicyclohexylcarbodiimide
DCM dichloromethane
DME 1 ,2-dimethoxyethane, dimethoxyethane
DMF Λ/,Λ/-dimethylformamide
DMSO dimethyl sulfoxide
EDC 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide hydrogen chloride
EtOAc ethyl acetate
EtOH ethanol
HOBt 1-hydroxybenzotriazole
MeOH methanol
NMP N-methyl-2-pyrrolidone
THF tetrahydrofuran
TFA trifluoroacetic acid
General Synthesis
The compounds of the present invention may be prepared by a variety of processes well known for the preparation of compounds of this type, for example as shown in the following reaction Schemes.
All starting materials in the following general syntheses are commercially available or may be obtained by conventional methods known to those skilled in the art.
A compound of formula (I) may be prepared according to scheme 1 below. Scheme 1
Figure imgf000011_0001
(
Step 1A : In this Step, an amide compound of formula (I) can be prepared by the coupling reaction of an amine compound of formula (II) with an acid compound of formula (III) in the presence or absence of a coupling reagent in an inert solvent. Suitable coupling reagents are those typically used in peptide synthesis including, for example, diimides (e.g., DCC, EDC, 2-ethoxy-N-ethoxycarbonyl-1 ,2-dihydroquinoline, BEP, CDI, BOP, diethyl azodicarboxylate-triphenylphosphine, diethylcyanophosphate, diethylphosphorylazide, 2-chloro-1-methylpyridinium iodide, N, N'-carbonyldiimidazole , benzotriazole-1-yl diethyl phosphate, ethyl chloroformate or isobutyl chloroformate. The reaction can be carried out in the presence of a base such as HOBt, N.N-diisopropylethylamine, N-methylmorpholine or triethylamine. The amide compound of formula (I) can also be formed via the reaction of a compound of formula (II) with an acylhalide, which is obtained by the reaction of a compound of formula (III) with halogenating agents such as oxalylchloride, phosphorus oxychloride or thionyl chloride. The reaction is normally and preferably effected in the presence of a solvent. There is no particular restriction on the nature of the solvent to be employed, provided that it has no adverse effect on the reaction or on the reagents involved and that it can dissolve the reagents, at least to some extent. Examples of suitable solvents include; acetone; nitromethane; DMF; NMP; sulfolane; DMSO; 2-butanone; acetonitrile; halogenated hydrocarbons such as DCM, dichloroethane or chloroform; and ethers such as THF or 1 ,4-dioxane. The reaction can take place over a wide range of temperatures, and the precise reaction temperature is not critical to the invention. The preferred reaction temperature will depend upon such factors as the nature of the solvent, and the starting material or reagent used. However, in general, we find it convenient to carry out the reaction at a temperature of from -200C to 100 0C, more preferably from about 0 0C to 60 0C. The time required for the reaction can also vary widely, depending on many factors, notably the reaction temperature and the nature of the reagents and solvent employed. However, provided that the reaction is effected under the preferred conditions outlined above, a period of 5 minutes to 1 week, more preferably 30 minutes to 24 hours, will usually suffice.
When R2 is methyl, a compound of formula (II) may be prepared from a compound of formula (IV), as illustrated by scheme 2. Scheme 2:
Figure imgf000012_0001
(VIII)
(M) wherein L is a suitable leaving group, and R' is t-butylsulfinyl, phenethyl, NH2, benzyl or diphenylmethyl.
Step 2A: In the above formula, a compound of formula (V) can be prepared by a coupling reaction of a compound of formula (IV) with 1-(vinyloxy)butane under basic conditions in the presence of a transition metal catalyst and additives in a solvent. Examples of suitable solvents include: protic solvents such as water, alcohols such as MeOH or EtOH, and co-solvents of water or alcohols as protic solvents mixed with THF, 1 ,4-dioxane, DMF or acetonitrile. This reaction can be carried out in the presence of a suitable catalyst. There is likewise no particular restriction on the nature of the catalyst used, and any catalysts commonly used in reactions of this type can equally be used here. Examples of such catalysts include: tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, copper(O), copper(l) acetate, copper(l) bromide, copper(l) chloride, copper(l) iodide, copper(l) oxide, copρer(ll) trifluoromethanesulfonatθ, copper(ll) acetate, copper(ll) bromide, copper(ll) chloride, copper(ll) iodide, copper(ll) oxide, copper(ll) trifluoromethanesulfonate, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibeπzylideneacetone)palladium(0), tris(dibeπzylideneacetone)dipalladium(0) or [1 ,1 '-bis(diphenylphosphino)ferrocene]palladium(ll) dichloride. Preferred catalysts are tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibenzylideπeacetone)palladium(0), tris(dibeπzylideneacetone)dipalladium(0) or [1 ,1'-bis(diphenylphosphino)ferrocene]palladium(ll) dichloride. This reaction can be carried out in the presence of a suitable additive agent. Examples of such additive agents include: triphenylphosphine, tri-te/t-butylphosphine,
1 ,2-bis(diphenylphosphino)ethane, 1 ,3-bis(diphenylphosphino)propane,
1 ,1 '-bis(diphenylphosphino)ferrocene, tri-2-furylphosphine, tri-o-tolylphosphine, 2-(dichlorohexylphosphino)biphenyl or triphenylarsine. This reaction can be carried out in the presence of bases such as potassium carbonate, sodium carbonate or cesium carbonate. The reaction can be carried out at a temperature of from 0 0C to 2000C, more preferably from 20 0C to 120 0C. Reaction time is, in general, from 5 minutes to 48 hours, more preferably 30 minutes to 24 hours will usually suffice.
Step 2B: In this step, a compound of formula (VII) can be prepared by coupling reaction of a compound of formula (V) with an amine of formula (Vl) in the presence of a dehydration reagent and/or HCI-MeOH and/or Lewis Acid. A preferred dehydration reagent includes sodium sulfate, magnesium sulfate, calcium sulfate or methylformate. Examples of suitable solvents include: THF; 1 ,4-dioxane; DMF; acetonitrile; alcohols such as MeOH or EtOH; halogenated hydrocarbons such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; or acetic acid. Reaction temperature is generally in the range of from 0 to 200 0C, preferably in the range of from 100 0C to 140 0C. Reaction time is, in general, from 1 minute to a day, preferably from 5 minutes to 1 hour. If necessary, microwave conditions may be applied to the reaction. Step 2C: In this step, a compound of formula (VIII) can be prepared by reduction of a compound of formula (VII). This reaction may be carried out in the presence of a suitable reducing agent such as diboran, boran-methyl sulfide complex, sodium borohydhde, lithium borohydride, sodium borohydride, or lithium aluminum hydride, in an inert solvent selected from THF and diethyl ether. Reaction temperature is generally in the range of from -100 to 250 0C, preferably in the range of from 0 0C to the reflux temperature, but if necessary, lower or higher temperatures can be employed. Reaction time is, in general, from 1 minute to a day, preferably from 20 minutes to 5 hours, however shorter or longer reaction times, if necessary, can be employed. The reduction may also be carried out under known hydrogenation conditions such as in the presence of a metal catalyst such as Raney nickel catalysts in the presence or absence of hydrazine, palladium catalysts or platinum catalysts under hydrogen atmosphere. This reaction may be carried out in an inert solvent such as MeOH, EtOH, and THF in the presence or absence of hydrogen chloride. If necessary, this reduction may be carried out under pressure in the range from about 0.5 to 10 kg/cm2, preferably in the range from 1 to 6 kg/cm2. Examples of suitable solvents are similar to those mentioned in Step 2B.
Step 2D: In this Step, a compound of the formula (II) can be prepared by deprotection and/or salt formation of a compound of formula (VIII) under acidic conditions in an inert solvent using the method described by D. Cogan el al (Journal of American Chemical Society, 1999, 121 , pp268-269). Suitbale acids include, for example, but not limited to hydrogen chloride, hydrogen bromide, trifluoromethane sulfonic acid, acetic acid or p-toluenesulfonic acid. The reaction may be also carried out under known hydrogenation conditions such as in the presence of a metal catalyst such as palladium-carbon catalyst or platinum catalysts under hydrogen atmosphere. This reaction may be carried out in an inert solvent such as MeOH, EtOH, and THF in the presence or absence of hydrogen chloride. If necessary, this reduction may be carried out under pressure in the range from about 0.5 to 10 kg/cm2, preferably in the range from 1 to 6 kg/cm2. Reaction temperature is generally in the range of from -1000C to 2500C, preferably in the range of from 0 0C to the reflux temperature, but if necessary, lower or higher temperatures can be employed. Reaction time is, in general, from 1 minute to 2 days, preferably from 20 minutes to 24 hours.
Alternatively, a compound of formula (II) may be prepared as illustrated in Scheme 3. Scheme 3:
Figure imgf000015_0001
Figure imgf000015_0002
(XIII) CO wherein R is C1-C2 alkyl; R' is t-butylsulfiniyl, phenethyl, NH2, benzyl or diphenylmethyl; and M is a suitable metal such as lithium, or MgZ, wherein Z is halogen. Step 3A: In this Step, a compound of formula (IX) may be prepared by reacting a compound of formula (IV) with carbon monoxide and an alcohol (e.g. MeOH or EtOH) in the presence of a catalyst and/or base in an inert solvent. Examples of suitable catalysts include palladium reagents, such as palladium acetate or palladium dibenzylacetone. Examples of suitable bases include N,N-diisopropylethylamine, N-methylmorpholine or triethylamine. If desired, this reaction may be carried out in the presence or absence of an additive such as 1 ,1'-bis(diphenylphosphino)ferrocene, triphenylphosphine or 1 ,3-bis-(diphenylphosphino)propane (DPPP). The reaction is normally and preferably effected in the presence of a solvent. There is no particular restriction on the nature of the solvent to be employed, provided that it has no adverse effect on the reaction or on the reagents involved and that it can dissolve the reagents, at least to some extent. Examples of suitable solvents include: acetone; nitromethane; DMF; sulfolane; DMSO; NMP; 2-butanone; acetonitrile; halogenated hydrocarbons such as DCM, dichloroethane or chloroform; or ethers, such as THF or 1 ,4-dioxane. The reaction can take place over a wide range of temperatures, and the precise reaction temperature is not critical to the invention. The preferred reaction temperature will depend upon such factors as the nature of the solvent, and the starting material or reagent used. However, in general, we find it convenient to carry out the reaction at a temperature of from -20 0C to 150 0C, more preferably from about 50 0C to 80 0C. The time required for the reaction may also vary widely, depending on many factors, notably the reaction temperature and the nature of the reagents and solvent employed. However, provided that the reaction is effected under the preferred conditions outlined above, a period of from 30 minutes to 24 hours, more preferably from 1 hour to 10 hours, will usually suffice.
Step 3B-1 : In this Step, an acid compound may be prepared by hydrolysis of a compound of formula (IX) in a solvent. The hydrolysis may be carried out by conventional procedures. In a typical procedure, the hydrolysis is carried out under basic conditions in the presence of water. Suitable bases include, for example, sodium hydroxide, potassium hydroxide or lithium hydroxide. Suitable solvents include, for example, alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol or ethylene gylcol; ethers such as THF, DME or 1 ,4-dioxane; amides such as DMF or hexamethylphosphorictriamide; or sulfoxides such as DMSO. This reaction may be carried out at a temperature in the range from -20 to 1000C, usually from 200C to 650C for from 30 minutes to 24 hours, usually from 60 minutes to 10 hours. The hydrolysis may also be carried out under acidic conditions, e.g. in the presence of hydrogen halides, such as hydrogen chloride and hydrogen bromide; sulfonic acids, such as p-toluenesulfonic acid and benzenesulfonic acid; pyridium p-toluenesulfonate; and carboxylic acid, such as acetic acid and trifluoroacetic acid. Suitable solvents include, for example, alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol, and ethylene gylcol; ethers such as THF, DME and 1 ,4-dioxane; amides such as DMF and hexamethylphosphorictriamide; and sulfoxides such as DMSO. This reaction may be carried out at a temperature in the range from -20 to 1000C, usually from 200C to 65°C for from 30 minutes to 24 hours, usually from 60 minutes to 10 hours. Step 3B-2: In this step, an amide compound of formula (X) can be prepared from the product of 3B-1 by the same procedure as described in Step 1. Step 3C: In this Step, a compound of formula (Xl) can be prepared by reaction of a compound of formula (X) with an organometallic reagent R1M. R 1 M can be prepared
from a halide compound of R1. For example, R 1 M, in which M represents MgZ, can be generated by stirring Mg and R1Z, dibromoethane and I2 at a temperature in the range of from 30 to 800C. This reaction may be carried out in the presence of an organometallic reagent or a metal. Examples of suitable organometallic reagents include alkyllithiums such as n-butyllithium, sec-butyllithium or tert-butyllithium; aryllithiums such as phenyllithium or lithium naphthylide. Examples of suitable metals include magnesium.
Preferred inert solvents include, for example, hydrocarbons, such as hexane; ethers, such as diethyl ether, diisopropyl ether, DME, THF or 1 ,4-dioxane; or mixtures thereof.
Reaction temperature is generally in the range of from -100 to 50 0C, preferably in the range of from -100 0C to room temperature. Reaction time is, in general, from 1 minute to a day, preferably from 1 hour to 10 hours.
Step 3D: In this step, a compound of formula (XII) can be prepared by coupling reaction of a compound of formula (Xl) with an amine of formula (Vl) by the method described in
Step 2B above.
Step 3E: In this Step, a compound of formula (XIII) can be prepared from a compound of formula (XII) by the method described in Step 2C above.
Step 3F: In this step, a compound of the formula (II) can be prepared from a compound of formula (XIII) by the method described in Step 2D above.
A compound of formula (III) may be prepared by the process illustrated in Scheme 4. Scheme 4
Figure imgf000017_0001
Figure imgf000017_0002
(XVIII)
Figure imgf000017_0003
R: (C1 -6)allyl C")
Step 4A: In this Step, a compound of formula (XV) can be prepared by N-substituted acrylation of a compound of formula (XIV) with dialkyl alkoxy methylenemalonate in a reaction inert solvent or without solvent. Examples of suitable solvents include alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol, and ethylene glycol; and ethers such as THF, DME, and 1 ,4-dioxaήe. The reaction can be carried out at a temperature in the range from 50°C to 150°C for from 30 minutes to 24 hours, usually from 60 minutes to 3 hours.
Step 4B: In this Step, a compound of formula (XVI) can be prepared by thermal cyclization of a compound of formula (XV) in a reaction inert solvent. Examples of suitable solvents include ethers such as phenyl ether. This reaction can be carried out at a temperature in the range from 200 to 3000C for from 30 minutes to 24 hours, usually 2500C for from 30 minutes to 5 hours. (Journal of Medicinal chemistry, 1998,VoI 41 , No25. pp4918-4926.
Step 4C: In this Step, a compound of formula (XVII) can be prepared by chlorination of a compound of formula (XVI). The reaction is carried out under chlorination conditions with a chlorination reagent in a reaction inert solvent or without solvent. Examples of suitable solvents include THF; 1 ,4-dioxane; DMF; acetonitrile; halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; and acetic acid. Examples of suitable halogenating reagents include phosphorus oxyhalide such as phosphorus oxychloride; and phosphorus oxybromide. The reaction can be carried out at a temperature of from 00C to 200 0C, more preferably from ambient temperature to 150 0C. Reaction times are, in general, from 5 minutes to 48 hours, more preferably from 30 minutes to 6 hours will usually suffice.
Step 4D: In this Step, a dehalogenated compound of formula (XVIII) can be prepared by hydrogenation of a compound of formula (XVII) in a solvent. Hydrogenation reaction is carried out under, for example, known hydrogenolysis conditions in the presence of a metal catalyst under a hydrogen atmosphere or in the presence of hydrogen sources such as formic acid or ammonium formate in a reaction inert solvent. If desired, the reaction may be carried out under basic conditions, for example, in the presence of triethylamine. Preferred reagents are selected from, for example, nickel catalysts such as Raney nickel, palladium-carbon, palladiumhydroxide-carbon, platinumoxide, platinum-carbon, ruthenium-carbon, rhodium-aluminumoxide and tris[triphenyphosphine] rhodiumchloride. Examples of suitable reaction inert aqueous or non-aqueous organic solvents include alcohols, such as MeOH, EtOH; ethers, such as THF or 1 ,4-dioxane; acetone; dimethylformamide; halogenated hydrocarbons, such as DCM, dichloroethane or chloroform; and acetic acid; or mixtures thereof. The reaction can be carried out at a temperature in the range from 20°C to 100°C, preferably in the range from 20°C to 60°C.
Reaction times are, in general, from 10 minutes to 48 hours, preferably from 30 minutes to 24 hours. This reaction can be carried out under hydrogen atmosphere at a pressure ranging from 1 to 100 atom, preferably from 1 to 10 atm. The preferred conditions are the use of 5 or 10% palladium-carbon at from 20 to 25°C for 1 to 24 hours under hydrogen atmosphere using a balloon.
Step 4E: In this Step, an acid compound of formula (III) can be prepared by hydrolysis of a compound of formula (XVIII) in a solvent by the method as described in Step 3B-1.
Alternatively, a compound of formula (III) may be prepared by the process illustrated in Scheme 5.
Step 5B
Figure imgf000019_0001
Figure imgf000019_0002
(XXI) (III)
Step 5A: In this Step, a N-oxide compound of formula (XX) can be prepared by oxidation of a compound of formula (XIX) in a reaction inert solvent. The oxidation reaction may be carried out in the absence or presence of an additive agent in a reaction inert solvent. Examples of preferred oxidation reagents include meta-chloroperbenzoic acid (mCPBA), hydrogen peroxide and peracetic acid. Examples of preferred reaction inert solvents include halogenated hydrocarbons, such as methylene chloride, chloroform, carbon tetrachloride and dichloroethane; ethers, such as diethyl ether, diisopropyl ether, DME, THF and 1 ,4-dioxane; acetonitrile; acetic acid; and water; or mixtures thereof. Reaction temperatures are generally in the range from 0°C to 250°C, more preferably in the range from 0°C to 100°C. Reaction times are, in general, from 1 minute to 10 days, more preferably from 20 minutes to 6 hours. This reaction may be carried out in the presence of a suitable catalyst. There is likewise no particular restriction on the nature of the catalyst used, and any catalyst commonly used in reactions of this type may equally be used here. Examples of such catalysts include methyltrioxorhenium(VII), tungstic acid and sodium tungstate dehydrate. Step 5B: In this Step, a cyano compound of formula (XXI) can be prepared by cyanation of a compound of formula (XX) in a reaction inert solvent. Examples of preferred cyanation reagents include trimethylsilanecarbonitrile (TMSCN), the combination of trimethylchlorosilane and sodium cyanide, and the combination of acylating agents such as N,N-dimethylcarbamoyl chloride with trimethylsilanecarbonitrile (TMSCN). A preferred cyanation reagent is trimethylsilanecarbonitrile (TMSCN) in the presence of a base such triethylamine in a reaction inert solvent. Examples of preferred reaction inert solvents include halogenated hydrocarbons, such as methylene chloride, chloroform, carbon tetrachloride and dichloroethane; ethers, such as diethyl ether, DME, THF and 1 ,4-dioxane; acetonitrile; DMF; DMSO; or mixtures thereof. Reaction temperatures are generally in the range from 00C to 250 0C, more preferably in the range from 0°C to 100°C. Reaction times are, in general, from 1 minute to 10 days, more preferably from 20 minutes to 24 hours.
Step 5C: In this Step, an acid compound of formula (III) can be prepared by hydrolysis of a cyano compound of formula (XXI) in a solvent. The hydrolysis can be carried out by conventional procedures. In a typical procedure, the hydrolysis may be carried out under basic conditions, e.g. in the presence of sodium hydroxide, potassium hydroxide or lithium hydroxide. Examples of suitable solvents include alcohols such as MeOH, EtOH, propanol, butanol, 2-methoxyethanol, and ethylene gylcol; ethers such as THF, DME, and 1 ,4-dioxane; amides such as DMF and hexamethylphosphorictriamide; and sulfoxides such as DMSO. Preferable solvents are MeOH, EtOH, propanol, THF, DME, 1 ,4-dioxane, DMF and DMSO. This reaction can be carried out at a temperature in the range from -20 to 150°C, usually from 20°C to 100°C for 30 minutes to 24 hours, usually 60 minutes to 10 hours.
When X4 and X5 are both CH, a compound of formula (III) may be prepared by the process illustrated in Scheme 6. Scheme 6
Figure imgf000021_0001
Figure imgf000021_0002
Figure imgf000021_0003
Step-6A
In this Step, a compound of formula (XXIII) can be prepared by O-dealkylation of a compound of formula (XXII). The reaction is carried out under O-dealkylation conditions with a dealkylating reagent in a reaction inert solvent or without solvent. Examples of suitable solvents include halogenated hydrocarbons, such as dichloromethane, 1 ,2-dichloroethane, chloroform or carbon tetrachloride. Examples of suitable dealkylating reagents include boron halides, such as boron trichloride and boron tribromide. The reaction can be carried out at a temperature of from O 0C to 2000C, more preferably from ambient temperature to 60 0C. Reaction times are, in general, from 5 minutes to 48 hours, more preferably 30 minutes to 6 hours will usually suffice. Step-6B
In this step, a compound of formula (XXIV) can be prepared by trifluoromethane sulfonation reaction of a compound of formula (XXIII) using trifluoromethane sulfonic acid anhydrate under basic conditions in an inert solvent. A preferred base is selected from, for example, but not limited to: an alkali or alkaline earth metal hydroxide, alkoxide, carbonate, halide or hydride, such as sodium hydroxide, potassium hydroxide, sodium methoxide, sodium ethoxide, potassium terf-butoxide, sodium carbonate, potassium carbonate, potassium fluoride, sodium hydride or potassium hydride; or an amine such as triethylamine, tributylamine, diisopropylethylamine, 2,6-lutidine, pyridine or dimethylaminopyridine. Examples of suitable solvents include: toluene; xylene; DME; DMSO; THF; 1 ,4-dioxane; DMF; acetonitrile; halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; and diethylether. Reaction temperatures are generally in the range of from -78 0C to 2000C, preferably in the range of from 0 0C to room temperature. Reaction times are, in general, from 1 minute to a day, preferably from 1 hour to 20 hours. Step-6C
In this step, a compound of formula (XXV) can be prepared by palladium catalyzed reaction of a compound of formula (XXIV) using a suitable boron reagent in an inert solvent. Examples of suitable solvents include: THF; 1 ,4-dioxane; DMF; acetonitrile; alcohols, such as methanol or ethanol; halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride; and diethylether; in the presence or absence of an aqueous base such as aqueous KOH, NaOH, LiOH or K2CO3. Suitable boron reagents include, for example, pinacolborane and bis(neopentyl glycolato)diboron. Examples of suitable transition metal catalysts include: tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, copper(O), copper(l) acetate, copper(l) bromide, copper(l) chloride, copper(l) iodide, copper(l) oxide, copper(ll) trifluoromethanesulfonate, copper(ll) acetate, copper(ll) bromide, copper(ll) chloride, copper(ll) iodide, copper(ll) oxide, copper(ll) trifluoromethanesulfonate, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibenzyiideneacetone)palladium(0), tris(dibenzylideneacetone)dipalladium(0) and
[1 ,1 '-bis(diphenylphosphino)ferrocene]palladium(ll) dichloride. Preferred catalysts are tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)palladium(ll) chloride, palladium(ll) acetate, palladium(ll) chloride, bisacetonitriledichloropalladium(O), bis(dibenzylideneacetone)palladium(0), tris(dibenzylideneacetone)dipalladium(0) and [1 ,1 '-bis(diphenylphosphino)ferrocene]palladium(ll) dichloride. This reaction can be carried out in the presence of a suitable additive agent. Examples of such additive agents include: triphenylphosphine, tri-fe/t-butylphosphine,
1 ,1'-bis(diphenylphosphino)ferrocene, tri-2-furylphosphine, tri-o-tolylphosphine, 2-(dichlorohexylphosphino)biphenyl, triphenylarsine, tetrabutylammonium chloride, tetrabutylammonium fluoride, lithium acetate, lithium chloride, triethylamine, potassium acetate, potassium sodium methoxide, sodium hydroxide, carbonate, sodium bicarbonate and/or sodium iodide. The reaction can be carried out at a temperature of from 0 0C to 200 0C, more preferably from 20 0C to 120 0C. Reaction times are, in general, from 5 minutes to 96 hours, more preferably from 30 minutes to 24 hours.
Step-6D
In this step, a compound of formula (XXVI) can be prepared by iodination of a compound of formula (XXV). This reaction was carried out according to the method described in
Synthesis, 2005, No4, pp 0547-0550.
Step-6E
In this step, a trifluoromethyl compound of formula (XXVII) can be prepared from a compound of formula (XXVI) by trifluoromethylation using a trifluoromethyl anion source in an inert solvent. Examples of suitable solvents include: THF; 1 ,4-dioxane; DMF; acetonitrile; and halogenated hydrocarbons, such as DCM, 1 ,2-dichloroethane, chloroform or carbon tetrachloride. Examples of suitable trifluoromethyl anion source reagents include for example, the combination of CF2CI2(Or CF2Br2)AZn or Cd/Cul,
ICF2SO2F/Cu, FSO2CF2CO2Me,/Cul, CF3CO2Na/Cul and CI2CF2CO2Me/KF/Cul. The reaction can be carried out at a temperature of from 00C to 2000C, more preferably from
20 0C to 120 0C. Reaction times are, in general, from 5 minutes to 96 hours, more preferably from 30 minutes to 24 hours.
Step-6F
In this step, a trifluoromethyl compound of formula (XXVIII) can be prepared from a compound of (XXVII) by alkali hydrolysis as disclosed in Step 3B-1.
The various general methods described above may be useful for the introduction of the desired groups at any stage in the stepwise formation of the required compound, and it will be appreciated that these general methods can be combined in different ways in such multi-stage processes. The sequence of the reactions in multi-stage processes should of course be chosen so that the reaction conditions used do not affect groups in the molecule which are desired in the final product. Method for assessing biological activities Human VR1 antagonist assay
VR1 antagonistic activity can be determined by the Ca2+ imaging assay using human VR1 highly expressing cells. The cells that highly express human VR1 receptors are obtainable from several different conventional methods. The one standard method is cloning from human Dorsal Root Ganglion (DRG) or kidney according to the methods such as described in the journal article; Nature, 389, pp816-824, 1997. Alternatively VR1 receptors highly expressing human keratinocytes are also known and published in the journal article (Biochemical and Biophysical Research Communications, 291 , pp124-129, 2002). In this article, human keratinocytes demonstrated VR1 mediated intracellular Ca2+ increase by addition of capsaicin. Furthermore, the method to up regulate human VR1 gene, which is usually a silent gene or don't produce detectable level of VR1 receptors, is also available to obtain propriety cells. Such genetic modification method was described in detail; Nat. Biotechnol., 19, pp440-445, 2001.
The cells that express human VR1 receptors were maintained in culture flask at 37 0C in an environment containing 5% CO2 until use in the assay. The intracellular Ca2+ imaging assay to determine VR1 antagonistic activities were done by following procedures.
The culture medium was removed from the flask and fura-2/AM fluorescent calcium indicator was added to the flask at a concentration of 5 μM in the medium. The flask was placed in CO2 incubator and incubated for 1 hour. Then the cells expressing the human VR1 receptors were detached from the flask follow by washing with phosphate buffer saline, PBS(-) and re-suspended in assay buffer. The 80 μ\ of aliquot of cell suspension (3.75x105 cells/ml) was added to the assay plate and the cells were spun down by centrifuge (950 rpm, 20 0C, 3 minutes).
The compounds of the examples were tested in the Human VR1 antagonist assay described above. The IC50 values are presented in the following table. Table 1
Figure imgf000024_0001
Figure imgf000024_0002
Capsaicin stimulation assay
The capsaicin-induced changes in the intracellular calcium concentration were monitored using FDSS 6000 (Hamamatsu Photonics, Japan), a fluorometric imaging system. The cell suspension in Krebs-Ringer HEPES (KRH) buffer (115 mM NaCI, 5.4 imM KCI, 1 mM MgSO4, 1.8 mM CaCI2, 11 mM D-Glucose, 25 mM HEPES, 0.96 mM Na2HPO4, pH 7.3) were pre-incubated with varying concentrations of the test compounds or KRH buffer (buffer control) for 15 minutes at room temperature under the dark condition. Then capsaicin solution, which gives 300 nM in assay mixture, was automatically added to the assay plate by the FDSS 6000. Acid stimulation assay
The Acid-induced changes in the intracellular calcium concentration were monitored using FDSS 6000 (Hamamatsu Photonics, Japan), a fluorometric imaging system. The cell suspension in resting buffer (HBSS supplemented with 1OmM HEPES, pH 7.4) were pre-incubated with varying concentrations of the test compounds or resting buffer (buffer control) for 15 minutes at room temperature under the dark condition. The cells were automatically added the stimulating solution (HBSS supplemented with MES, final assay buffer pH5.8) by the FDSS 6000. The IC5O values of VR1 antagonists were determined from the half of the increase demonstrated by buffer control samples after acidic stimulation. Determination of antagonist activity
The monitoring of the changes in the fluorescence signals (λex = 340 nm/ 380 nm, λem = 510 - 520 nm) was initiated at 1 minute prior to the addition of capsaicin solution or acidic buffer and continued for 5 minute. The IC5O values of VR1 antagonists were determined from the half of the increase demonstrated by buffer control samples after agonist stimulation. Chronic Constriction Injury Model (CCI Model)
Male Sprague-Dawley rats (270-300 g; B.W., Charles River, Tsukuba, Japan) were used. The chronic constriction injury (CCI) operation was performed according to the method described by Bennett and Xie (Bennett, G.J. and Xie, YK. Pain, 33:87-107, 1988). Briefly, animals were anesthetized with sodium pentobarbital (64.8 mg/kg, i.p.) and the left common sciatic nerve was exposed at the level of the middle of the thigh by blunt dissection through biceps femoris. Proximal to the sciatic's trifurcation was freed of adhering tissue and 4 ligatures (4-0 silk) were tided loosely around it with about 1 mm space. Sham operation was performed as same as CCI surgery except for sciatic nerve ligation. Two weeks after surgery, mechanical allodynia was evaluated by application of von Frey hairs (VFHs) to the plantar surface of the hind paw. The lowest amount of force of VFH required to elicit a response was recorded as paw withdrawal threshold (PWT). VFH test was performed at 0.5, 1 and 2 hr post-dosing. Experimental data were analyzed using Kruskal-Wallis test followed by Dunn's test for multiple comparisons or Mann-Whitney U-test for paired comparison. Caco-2 permeability
Caco-2 permeability was measured according to the method described in Shiyin Yee, Pharmaceutical Research, 763 (1997).
Caco-2 cells were grown on filter supports (Falcon HTS multiwell insert system) for 14 days. Culture medium was removed from both the apical and basolateral compartments and the monolayers were preincubated with pre-warmed 0.3 ml apical buffer and 1.0 ml basolateral buffer for 0.75 hour at 37°C in a shaker water bath at 50 cycles/min. The apical buffer consisted of Hanks Balanced Salt Solution, 25 mM D-glucose monohydrate, 20 mM MES Biological Buffer, 1.25 mM CaCI2 and 0.5 mM MgCI2 (pH 6.5). The basolateral buffer consisted of Hanks Balanced Salt Solution, 25 mM D-glucose monohydrate, 20 mM HEPES Biological Buffer, 1.25 mM CaCI2 and 0.5 mM MgCI2 (pH 7.4). At the end of the preincubation, the media was removed and test compound solution (10μM) in buffer was added to the apical compartment. The inserts were moved to wells containing fresh basolateral buffer and incubated for 1 hr. Drug concentration in the buffer was measured by LC/MS analysis.
Flux rate (F, mass/time) was calculated from the slope of cumulative appearance of substrate on the receiver side and apparent permeability coefficient (Papp) was calculated from the following equation.
Papp (cm/sec) = (F * VD) / (SA * MD) where SA is surface area for transport (0.3 cm2), VD is the donor volume (0.3ml), MD is the total amount of drug on the donor side at t = 0. All data represent the mean of 2 inserts. Monolayer integrity was determined by Lucifer Yellow transport.
Parallel artificial membrane permeation assay ( PAMPA )
Experiments were performed in 96-well acceptor and donor plates. Such 96-well system was described in Journal of Medicinal Chemistry, 1998, vol.41, No.7, 1007-1010. 4% phosphatidylcholine and 1% stearic acid in dodecane were used as artificial membrane material. The acceptor plate (96 well hydrophobic filter plate (MAIP N45, Millipore)) was prepared by adding 5 μL of artificial membrane material on the top of the filter and the plate was filled with 250 μL of 2-(N-morpholino)ethanesulfonic acid (MES) buffered Hank's balanced salt solution (HBSS) (pH 6.5). The donor plate (Transport
Receiver plate (MATRN PS50, Millipore)) was filled with 300 μL of MES buffered HBSS (pH 6.5) containing 10 //M of the test compounds. The acceptor plate was placed onto the donor plate to form a "sandwich" and was incubated at 3O0C for 2.5 hours. After the incubation period, acceptor, donor and initial donor solution (reference) were analyzed via LC-MS/MS. Data were reported as the effective permeability value in cm X 106/sec and the membrane retention value. Human dofetilide binding
Cell paste of HEK-293 cells expressing the HERG product can be suspended in 10-fold volume of 50 mM Tris buffer adjusted at pH 7.5 at 25 0C with 2 M HCI containing 1 mM MgCI2, 10 mM KCI. The cells were homogenized using a Polytron homogenizer (at the maximum power for 20 seconds) and centrifuged at 48,00Og for 20 minutes at 4°C. The pellet was resuspended, homogenized and centrifuged once more in the same manner. The resultant supernatant was discarded and the final pellet was resuspended (10-fold volume of 50 mM Tris buffer) and homogenized at the maximum power for 20 seconds. The membrane homogenate was aliquoted and stored at -8O0C until use. An aliquot was used for protein concentration determination using a Protein Assay Rapid Kit and ARVO SX plate reader (Wallac). All the manipulation, stock solution and equipment were kept on ice at all time. For saturation assays, experiments were conducted in a total volume of 200 μ\. Saturation was determined by incubating 20 μ\ of [3H]-dofetilide and 160 μ\ of membrane homogenates (20-30 μg protein per well) for 60 min at room temperature in the absence or presence of 10 μM dofetilide at final concentrations (20 //I) for total or nonspecific binding, respectively. All incubations were terminated by rapid vacuum filtration over poϊyetherimide (PEI) soaked glass fiber filter papers using Skatron cell harvester followed by two washes with 50 mM Tris buffer (pH 7.5 at 25 0C). Receptor-bound radioactivity was quantified by liquid scintillation counting using Packard LS counter.
For the competition assay, compounds were diluted in 96 well polypropylene plates as 4-point dilutions in semi-log format. All dilutions were performed in DMSO first and then transferred into 50 mM Tris buffer (pH 7.5 at 25 0C) containing 1 mM MgCI2, 10 mM KCI so that the final DMSO concentration became equal to 1%. Compounds were dispensed in triplicate in assay plates (4 μ\). Total binding and nonspecific binding wells were set up in 6 wells as vehicle and 10 //M dofetilide at final concentration, respectively. The radioligand was prepared at 5.6x final concentration and this solution was added to each well (36 μ\). The assay was initiated by addition of YSi poly-L-lysine Scintillation
Proximity Assay (SPA) beads (50 μ\, 1 mg/well) and membranes (110 μ\, 20 μg/well). Incubation was continued for 60 min at room temperature. Plates were incubated for a further 3 hours at room temperature for beads to settle. Receptor-bound radioactivity was quantified by counting Wallac MicroBeta plate counter. IHERR assay
HEK 293 cells which stably express the HERG potassium channel were used for electrophysiological study. The methodology for stable transfection of this channel in HEK cells can be found elsewhere (Z.Zhou et al., 1998, Biophysical Journal, 74, pp230-241). Before the day of experimentation, the cells were harvested from culture flasks and plated onto glass coverslips in a standard Minimum Essential Medium (MEM) medium with 10% Fetal Calf Serum (FCS). The plated cells were stored in an incubator at 37°C maintained in an atmosphere of 95%02/5%C02. Cells were studied between 15-28hrs after harvest.
HERG currents were studied using standard patch clamp techniques in the whole-cell mode. During the experiment the cells were superfused with a standard external solution of the following composition (mM); NaCI, 130; KCI, 4; CaCI2, 2; MgCI2, 1 ; Glucose, 10; HEPES, 5; pH 7.4 with NaOH. Whole-cell recordings was made using a patch clamp amplifier and patch pipettes which have a resistance of 1-3MOhm when filled with the standard internal solution of the following composition (mM); KCI, 130; MgATP, 5; MgCI2, 1.0; HEPES, 10; EGTA 5, pH 7.2 with KOH. Only those cells with access resistances below 15MΩ and seal resistances >1 GΩ was accepted for further experimentation. Series resistance compensation was applied up to a maximum of 80%. No leak subtraction was done. However, acceptable access resistance depended on the size of the recorded currents and the level of series resistance compensation that can safely be used. Following the achievement of whole cell configuration and sufficient time for cell dialysis with pipette solution (>5min), a standard voltage protocol was applied to the cell to evoke membrane currents. The voltage protocol is as follows. The membrane was depolarized from a holding potential of -8OmV to +4OmV for 1000ms. This was followed by a descending voltage ramp (rate 0.5mV msec"1) back to the holding potential. The voltage protocol was applied to a cell continuously throughout the experiment every 4 seconds (0.25Hz). The amplitude of the peak current elicited around -4OmV during the ramp was measured. Once stable evoked current responses were obtained in the external solution, vehicle (0.5% DMSO in the standard external solution) was applied for 10-20 min by a peristalic pump.
Provided there were minimal changes in the amplitude of the evoked current response in the vehicle control condition, the test compound of either 0.3, 1 , 3, 10//M was applied for a 10 min period. The 10 min period included the time which supplying solution was passing through the tube from solution reservoir to the recording chamber via the pump. Exposing time of cells to the compound solution was more than 5min after the drug concentration in the chamber well reached the attempting concentration. There was a subsequent wash period of a 10-20min to assess reversibility. Finally, the cells were exposed to high dose of dofetilide (5DM), a specific IKr blocker, to evaluate the insensitive endogenous current.
All experiments were performed at room temperature (23±1QC). Evoked membrane currents were recorded on-line on a computer, filtered at 500-1 KHz (Bessel -3dB) and sampled at 1-2 KHz using the patch clamp amplifier and a specific data analyzing software. Peak current amplitude, which occurred at around -4OmV, was measured off line on the computer.
The arithmetic mean of the ten values of amplitude was calculated under vehicle control conditions and in the presence of drug. Percent decrease of IN in each experiment was obtained by the normalized current value using the following formula: IN = (1- |D/IC )X100, where I0 is the mean current value in the presence of drug and Ic is the mean current value under control conditions. Separate experiments were performed for each drug concentration or time-matched control, and arithmetic mean in each experiment is defined as the result of the study. Drug-drug interaction assay
This method essentially involves determining the percent inhibition of product formation from fluorescence probe at 3μM of the each compound.
More specifically, the assay is carried out as follows. The compounds were pre-incubated with recombinant CYPs, 100 mM potassium phosphate buffer and fluorescence probe as substrate for 5min. Reaction was started by adding a warmed NADPH generating system, which consist of 0.5 mM NADP (expect; for 2D6 0.03 mM), 10 mM MgCI2, 6.2 mM DL-lsocitric acid and 0.5 U/ml lsocitric Dehydrogenase (ICD). The assay plate was incubated at 37°C (expect; for 1A2 and 3A4 at 300C) and taking fluoresce reading every minutes over 20 to 30min.
Data calculations were preceded as follows; 1. The slope (Time vs. Fluorescence units) was calculated at the linear region 2. The percentage of inhibition in compounds was calculated by the equation {(v0 - Vi) / Vo} x 100 = % inhibition
Wherein
V0 = rate of control reaction (no inhibitor)
Vj = rate of reaction in the presence of compounds. Table 2. Condition for drug-drug interaction assay.
Figure imgf000030_0001
Intrinsic Clearance
Test compounds (1 μM) were incubated with 1 mM MgCk, 1 nnM NADP+, 5 mM isocitric acid, 1 U/mL isocitric dehydrogenase and 0.8 mg/mL HLM(human liver microsomes) in 100 mM potassium phosphate buffer (pH 7.4) at 37°C on a number of 384-well plates. At several time points, a plate was removed from the incubator and the reaction was terminated with two incubation volumes of acetonitrile. The compound concentration in supernatant was measured by LC/MS/MS system. The intrinsic clearance value (Clint) was calculated using following equations: Clint (μl/min/mg protein) = (k x incubation volume) / Protein concentration k (min"1)= - slope of ln(concentration vs. time) Mono-lodoacetate (MIA)-induced OA model
Male 6-weeks-old Sprague-Dawley (SD, Japan SLC or Charles River Japan) rats were anesthetized with pentobarbital. Injection site (knee) of MIA was shaved and cleaned with 70% EtOH. Twenty-five μl of MIA solution or saline was injected in the right knee joint using a 29G needle. The effect of joint damage on the weight distribution through the right (damaged) and left (untreated) knee was assessed using an incapacitance tester (Linton Instrumentation, Norfolk, UK). The force exerted by each hind limb was measured in grams. The weight-bearing (WB) deficit was determined by a difference of weight loaded on each paw. Rats were trained to measure the WB once a week until 20 days post MIA-injection. Analgesic effects of compounds were measured at 21 days after the MIA injection. Before the compound administration, the "pre value" of WB deficit was measured. After the administration of compounds, attenuation of WB deficits was determined as analgesic effects. Complete Freund's adjuvant (CFA) induced thermal and mechanical hyperalgesia in rats
Thermal hyperalgesia
Male 6-week-old SD rats were used. Complete Freund's adjuvant (CFA, 300 Dg of Mycobacterium Tuberculosis H37RA (Difco, Ml) in 100 μL of liquid paraffin (Wako, Osaka, Japan)) was injected into the plantar surface of hind paw of the rats. Two days after CFA-injection, thermal hyperalgesia was determined by method described previously (Hargreaves et al., 1988) using the plantar test apparatus (Ugo-Basil, Varese, Italy). Rats were adapted to the testing environment for at least 15 min prior to any stimulation. Radiant heat was applied to the plantar surface of hind paw and paw withdrawal latencies (PWL, seconds) were determined. The intensity of radiant heat was adjusted to produce the stable PWL of 10 to 15 seconds. The test compound was administered in a volume of 0.5 mL per 100 g body weight. PWL were measured after 1 , 3 or 5 hours after drug administration. Mechanical hyperalgesia
Male 4-week-oId SD rats were used. CFA (300 μg of Mycobacterium Tuberculosis H37RA (Difco, Ml) in 100 μL of liquid paraffin (Wako, Osaka, Japan)) was injected into the plantar surface of hind paw of the rats. Two days after CFA-injection, mechanical hyperalgesia was tested by measuring paw withdrawal threshold (PWT, grams) to pressure using the analgesy-Meter (Ugo-Basil, Varese, Italy). The animals were gently restrained, and steadily increasing pressure was applied to the dorsal surface of a hind paw via a plastic tip. The pressure required to elicit paw withdrawal was determined. The test compound was administered in a volume of 0.5 mL per 100 g body weight. PWT were measured after 1 , 3 or 5 hours after drug administration. Drug Substance
Pharmaceutically acceptable salts of the compounds of formula (I) include the acid addition and base salts thereof.
Suitable acid addition salts are formed from acids which form non-toxic salts. Examples include acetate, aspartate, benzoate, besylate, bicarbonate/carbonate, bisulphate/sulphate, borate, camsylate, citrate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, hibenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, maleate, malonate, mesylate, methylsulphate, naphthylate, 2-napsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, saccharate, stearate, succinate, tartrate, tosylate and trifluoroacetate salts.
Suitable base salts are formed from bases which form non-toxic salts. Examples include the aluminum, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, potassium, sodium, tromethamine and zinc salts.
For a review on suitable salts, see "Handbook of Pharmaceutical Salts: Properties, Selection, and Use" by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).
A pharmaceutically acceptable salt of a compound of formula (I) may be readily prepared by mixing together solutions of the compound of formula (I) and the desired acid or base, as appropriate. The salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent. The degree of ionization in the salt may vary from completely ionized to almost non-ionized.
The compounds of the invention may exist in both unsolvated and solvated forms. The term 'solvate' is used herein to describe a molecular complex comprising the compound of the invention and one or more pharmaceutically acceptable solvent molecules, for example, EtOH. The term 'hydrate' is employed when said solvent is water.
Included within the scope of the invention are complexes such as clathrates, drug-host inclusion complexes wherein, in contrast to the aforementioned solvates, the drug and host are present in stoichiometric or non-stoichiometric amounts. Also included are complexes of the drug containing two or more organic and/or inorganic components which may be in stoichiometric or non-stoichiometric amounts. The resulting complexes may be ionized, partially ionized, or non-ionized. For a review of such complexes, see J Pharm Sci, 64 (8), 1269-1288 by Haleblian (August 1975).
Hereinafter all references to compounds of formula (I) include references to salts, solvates and complexes thereof and to solvates and complexes of salts thereof.
The compounds of the invention include compounds of formula (I) as hereinbefore defined, polymorphs, prodrugs, and isomers thereof (including optical, geometric and tautomeric isomers) as hereinafter defined and isotopically-labeled compounds of formula (I).
As stated, the invention includes all polymorphs of the compounds of formula (I) as hereinbefore defined.
Also within the scope of the invention are so-called 'prodrugs' of the compounds of formula (I). Thus certain derivatives of compounds of formula (I) which may have little or no pharmacological activity themselves can, when administered into or onto the body, be converted into compounds of formula (I) having the desired activity, for example, by hydrolytic cleavage. Such derivatives are referred to as 'prodrugs'. Further information on the use "of prodrugs may be found in 'Pro-drugs as Novel Delivery Systems, Vol. 14, ACS Symposium Series (T Higuchi and W Stella) and 'Bioreversible Carriers in Drug Design', Pergamon Press, 1987 (ed. E B Roche, American Pharmaceutical Association).
Prodrugs in accordance with the invention can, for example, be produced by replacing appropriate functionalities present in the compounds of formula (I) with certain moieties known to those skilled in the art as 'pro-moieties' as described, for example, in "Design of Prodrugs" by H Bundgaard (Elsevier, 1985).
Some examples of prodrugs in accordance with the invention include: (i) where the compound of formula (I) contains a carboxylic acid functionality (-COOH), an ester thereof, for example, replacement of the hydrogen with (C-i-CβJalkyl; (ii) where the compound of formula (I) contains an alcohol functionality (-OH), an ether thereof, for example, replacement of the hydrogen with (Ci-C6)alkanoyloxymethyl; and (iii) where the compound of formula (I) contains a primary or secondary amino functionality (-NH2 or -NHR where R is not H), an amide thereof, for example, replacement of one or both hydrogens with (d-C10)alkanoyl.
Further examples of replacement groups in accordance with the foregoing examples and examples of other prodrug types may be found in the aforementioned references.
Finally, certain compounds of formula (I) may themselves act as prodrugs of other compounds of formula (I).
The present invention includes all pharmaceutically acceptable isotopically-labelled compounds of formula (I) wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes suitable for inclusion in the compounds of the invention include isotopes of hydrogen, such as 2H and 3H, carbon, such as 11C, 13C and 14C, chlorine, such as 36CI, fluorine, such as 18F, iodine, such as 123I and 125I, nitrogen, such as 13N and 15N, oxygen, such as 15O, 17O and 18O, phosphorus, such as 32P, and sulphur, such as 35S. Certain isotopically-Iabelled compounds of formula (I), for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies. The radioactive isotopes tritium, i.e. 3H, and carbon-14, i.e. 14C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection. Substitution with heavier isotopes such as deuterium, i.e. 2H, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances. Substitution with positron emitting isotopes, such as 11C, 18F, 15O and 13N, can be useful in Positron Emission Topography (PET) studies for examining substrate receptor occupancy. Isotopically-labeled compounds of formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples and Preparations using an appropriate isotopically-labeled reagents in place of the non-labeled reagent previously employed.
Pharmaceutically acceptable solvates in accordance with the invention include those wherein the solvent of crystallization may be isotopically substituted, e.g. D2O, d6-acetone, d6-DMSO.
Compounds of the invention intended for pharmaceutical use may be administered as crystalline or amorphous products. They may be obtained, for example, as solid plugs, powders, or films by methods such as precipitation, crystallization, freeze drying, or spray drying, or evaporative drying. Microwave or radio frequency drying may be used for this purpose.
They may be administered alone or in combination with one or more other compounds of the invention or in combination with one or more other drugs (or as any combination thereof). Generally, they will be administered as a formulation in association with one or more pharmaceutically acceptable excipients. The term "excipient" is used herein to describe any ingredient other than the compound(s) of the invention. The choice of excipient will to a large extent depend on factors such as the particular mode of administration, the effect of the excipient on solubility and stability, and the nature of the dosage form.
Pharmaceutical compositions suitable for the delivery of compounds of the present invention and methods for their preparation will be readily apparent to those skilled in the art. Such compositions and methods for their preparation may be found, for example, in 'Remington's Pharmaceutical Sciences', 19th Edition (Mack Publishing Company, 1995).
ORAL ADMINISTRATION
The compounds of the invention may be administered orally. Oral administration may involve swallowing, so that the compound enters the gastrointestinal tract, or buccal or sublingual administration may be employed by which the compound enters the blood stream directly from the mouth.
Formulations suitable for oral administration include solid formulations such as tablets, capsules containing particulates, liquids, or powders, lozenges (including liquid-filled), chews, multi- and nano-particulates, gels, solid solution, liposome, films (including muco-adhesive), ovules, sprays and liquid formulations.
Liquid formulations include suspensions, solutions, syrups and elixirs. Such formulations may be employed as fillers in soft or hard capsules and typically comprise a carrier, for example, water, EtOH, polyethylene glycol, propylene glycol, methylcellulose, or a suitable oil, and one or more emulsifying agents and/or suspending agents. Liquid formulations may also be prepared by the reconstitution of a solid, for example, from a sachet.
The compounds of the invention may also be used in fast-dissolving, fast-disintegrating dosage forms such as those described in Expert Opinion in Therapeutic Patents, 11 (6), 981-986 by Liang and Chen (2001).
For tablet dosage forms, depending on dose, the drug may make up from 1 wt% to 80 wt% of the dosage form, more typically from 5 wt% to 60 wt% of the dosage form. In addition to the drug, tablets generally contain a disintegrant. Examples of disintegrants include sodium starch glycolate, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, croscarmellose sodium, crospovidone, polyvinylpyrrolidone, methyl cellulose, microcrystalline cellulose, lower alkyl-substituted hydroxypropyl cellulose, starch, pregelatinised starch and sodium alginate. Generally, the disintegrant will comprise from 1 wt% to 25 wt%, preferably from 5 wt% to 20 wt% of the dosage form.
Binders are generally used to impart cohesive qualities to a tablet formulation. Suitable binders include microcrystalline cellulose, gelatin, sugars, polyethylene glycol, natural and synthetic gums, polyvinylpyrrolidone, pregelatinised starch, hydroxypropyl cellulose and hydroxypropyl methylcellulose. Tablets may also contain diluents, such as lactose (monohydrate, spray-dried monohydrate, anhydrous and the like), mannitol, xylitol, dextrose, sucrose, sorbitol, microcrystalline cellulose, starch and dibasic calcium phosphate dihydrate.
Tablets may also optionally comprise surface active agents, such as sodium lauryl sulfate and polysorbate 80, and glidants such as silicon dioxide and talc. When present, surface active agents may comprise from 0.2 wt% to 5 wt% of the tablet, and glidants may comprise from 0.2 wt% to 1 wt% of the tablet.
Tablets also generally contain lubricants such as1 magnesium stearate, calcium stearate, zinc stearate, sodium stearyl fumarate, and mixtures of magnesium stearate with sodium lauryl sulphate. Lubricants generally comprise from 0.25 wt% to 10 wt%, preferably from 0.5 wt% to 3 wt% of the tablet.
Other possible ingredients include anti-oxidants, colorants, flavouring agents, preservatives and taste-masking agents.
Exemplary tablets contain up to about 80% drug, from about 10 wt% to about 90 wt% binder, from about 0 wt% to about 85 wt% diluent, from about 2 wt% to about 10 wt% disintegrant, and from about 0.25 wt% to about 10 wt% lubricant.
Tablet blends may be compressed directly or by roller to form tablets. Tablet blends or portions of blends may alternatively be wet-, dry-, or melt-granulated, melt congealed, or extruded before tabletting. The final formulation may comprise one or more layers and may be coated or uncoated; it may even be encapsulated.
The formulation of tablets is discussed in "Pharmaceutical Dosage Forms: Tablets, Vol. 1", by H. Lieberman and L. Lachman, Marcel Dekker, N.Y., N.Y., 1980 (ISBN 0-8247-6918-X).
Solid formulations for oral administration may be formulated to be immediate and/or modified controlled release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.
Suitable modified release formulations for the purposes of the invention are described in US Patent No. 6,106,864. Details of other suitable release technologies such as high energy dispersions and osmotic and coated particles are to be found in Verma et al, Pharmaceutical Technology On-line, 25(2), 1-14 (2001). The use of chewing gum to achieve controlled release is described in WO 00/35298. PARENTERAL ADMINISTRATION
The compounds of the invention may also be administered directly into the blood stream, into muscle, or into an internal organ. Suitable means for parenteral administration include .intravenous, intraarterial, intraperitoneal, intrathecal, intraventricular, intraurethral, intrastemal, intracranial, intramuscular and subcutaneous. Suitable devices for parenteral administration include needle (including microneedle) injectors, needle-free injectors and infusion techniques.
Parenteral formulations are typically aqueous solutions which may contain excipients such as salts, carbohydrates and buffering agents (preferably, to a pH of from 3 to 9), but, for some applications, they may be more suitably formulated as a sterile non-aqueous solution or as powdered a dried form to be used in conjunction with a suitable vehicle such as sterile, pyrogen-free water.
The preparation of parenteral formulations under sterile conditions, for example, by lyophilisation, may readily be accomplished using standard pharmaceutical techniques well known to those skilled in the art.
The solubility of compounds of formula (I) used in the preparation of parenteral solutions may be increased by the use of appropriate formulation techniques, such as the incorporation of solubility-enhancing agents. Formulations for use with needle-free injection administration comprise a compound of the invention in powdered form in conjunction with a suitable vehicle such as sterile, pyrogen-free water.
Formulations for parenteral administration may be formulated to be immediate and/or modified controlled release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release. Thus compounds of the invention may be formulated as a solid, semi-solid, or thixotropic liquid for administration as an implanted depot providing modified release of the active compound. Examples of such formulations include drug-coated stents and PGLA microspheres. TOPICAL ADMINISTRATION
The compounds of the invention may also be administered topically to the skin or mucosa, that is, dermally or transdermally. Typical formulations for this purpose include gels, hydrogels, lotions, solutions, creams, ointments, dusting powders, dressings, foams, films, skin patches, wafers, implants, sponges, fibres, bandages and microemulsions. Liposomes may also be used. Typical carriers include alcohol, water, mineral oil, liquid petrolatum, white petrolatum, glycerin, polyethylene glycol and propylene glycol. Penetration enhancers may be incorporated - see, for example, J Pharm Sci, 88 (10), 955-958 by Finnin and Morgan (October 1999).
Other means of topical administration include delivery by electroporation, iontophoresis, phonophoresis, sonophoresis and microneedle or needle-free (e.g. Powderject™, Bioject™, etc.) injection.
Formulations for topical administration may be formulated to be immediate and/or modified controlled release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release. INHALED/INTRANASAL ADMINISTRATION
The compounds of the invention can also be administered intranasally or by inhalation, typically in the form of a dry powder (either alone, as a mixture, for example, in a dry blend with lactose, or as a mixed component particle, for example, mixed with phospholipids, such as phosphatidylcholine) from a dry powder inhaler or as an aerosol spray from a pressurized container, pump, spray, atomiser (preferably an atomiser using electrohydrodynamics to produce a fine mist), or nebuliser, with or without the use of a suitable propellant, such as 1 ,1 ,1 ,2-tetrafluoroethane or
1 ,1 ,1 ,2,3,3,3-heptafluoropropane. For intranasal use, the powder may comprise a bioadhesive agent, for example, chitosan or cyclodextrin.
The pressurized container, pump, spray, atomizer, or nebuliser contains a solution or suspension of the compound(s) of the invention comprising, for example, EtOH, aqueous EtOH, or a suitable alternative agent for dispersing, solubilising, or extending release of the active, a propellant(s) as solvent and an optional surfactant, such as sorbitan trioleate, oleic acid, or an oligolactic acid.
Prior to use in a dry powder or suspension formulation, the drug product is micronised to a size suitable for delivery by inhalation (typically less than 5 microns). This may be achieved by any appropriate comminuting method, such as spiral jet milling, fluid bed jet milling, supercritical fluid processing to form nanoparticles, high pressure homogenisation, or spray drying.
Capsules (made, for example, from gelatin or HPMC), blisters and cartridges for use in an inhaler or insufflator may be formulated to contain a powder mix of the compound of the invention, a suitable powder base such as lactose or starch and a performance modifier such as /-leucine, mannitol, or magnesium stearate. The lactose may be anhydrous or in the form of the monohydrate, preferably the latter. Other suitable excipients include dextran, glucose, maltose, sorbitol, xylitol, fructose, sucrose and trehalose.
A suitable solution formulation for use in an atomiser using electrohydrodynamics to produce a fine mist may contain from 1μg to 20mg of the compound of the invention per actuation and the actuation volume may vary from 1μl to 100μl. A typical formulation may comprise a compound of formula (I), propylene glycol, sterile water, EtOH and sodium chloride. Alternative solvents which may be used instead of propylene glycol include glycerol and polyethylene glycol.
Suitable flavours, such as menthol and levomenthol, or sweeteners, such as saccharin or saccharin sodium, may be added to those formulations of the invention intended for inhaled/intranasal administration.
Formulations for inhaled/intranasal administration may be formulated to be immediate and/or modified controlled release using, for example, poly(DL-lactic-coglycolic acid (PGLA). Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.
In the case of dry powder inhalers and aerosols, the dosage unit is determined by means of a valve which delivers a metered amount. Units in accordance with the invention are typically arranged to administer a metered dose or "puff" containing from 1 μg to 10mg of the compound of formula (I). The overall daily dose will typically be in the range 1 μg to 10 mg which may be administered in a single dose or, more usually, as divided doses throughout the day. RECTAL/I NTRA VAGINAL ADMINISTRATION
The compounds of the invention may be administered rectally or vaginally, for example, in the form of a suppository, pessary, or enema. Cocoa butter is a traditional suppository base, but various alternatives may be used as appropriate.
Formulations for rectal/vaginal administration may be formulated to be immediate and/or modified controlled release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release. OTHER TECHNOLOGIES
The compounds of the invention may be combined with soluble macromolecular entities, such as cyclodextrin and suitable derivatives thereof or polyethylene glycol-containing polymers, in order to improve their solubility, dissolution rate, taste-masking, bioavailability and/or stability for use in any of the aforementioned modes of administration.
Drug-cyclodextrin complexes, for example, are found to be generally useful for most dosage forms and administration routes. Both inclusion and non-inclusion complexes may be used. As an alternative to direct complexation with the drug, the cyclodextrin may be used as an auxiliary additive, i.e. as a carrier, diluent, or solubiliser. Most commonly used for these purposes are alpha-, beta- and gamma-cyclodextrins, examples of which may be found in International Patent Applications Nos. WO 91/11172, WO 94/02518 and WO 98/55148. DOSAGE
For administration to human patients, the total daily dose of the compounds of the invention is typically in the range 0.1 mg to 3000 mg, preferably from 1 mg to 500mg, depending, of course, on the mode of administration. For example, oral administration may require a total daily dose of from 0.1 mg to 3000 mg, preferably from 1mg to 500mg, while an intravenous dose may only require from 0.1 mg to 1000 mg, preferably from 0.1 mg to 300mg. The total daily dose may be administered in single or divided doses.
These dosages are based on an average human subject having a weight of about 65kg to 70kg. The physician will readily be able to determine doses for subjects whose weight falls outside this range, such as infants and the elderly.
For the avoidance of doubt, references herein to "treatment" include references to curative, palliative and prophylactic treatment.
A VR1 antagonist may be usefully combined with another pharmacologically active compound, or with two or more other pharmacologically active compounds, particularly in the treatment of pain. For example, a VR1 antagonist, particularly a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, as defined above, may be administered simultaneously, sequentially or separately in combination with one or more agents selected from:
• an opioid analgesic, e.g. morphine, heroin, hydromorphone, oxymorphone, levorphanol, levallorphan, methadone, meperidine, fentanyl, cocaine, codeine, dihydrocodeine, oxycodone, hydrocodone, propoxyphene, nalmefene, nalorphine, naloxone, naltrexone, buprenorphine, butorphanol, nalbuphine or pentazocine;
• a nonsteroidal antiinflammatory drug (NSAID), e.g. aspirin, diclofenac, diflusinal, etodolac, fenbufen, fenoprofen, flufenisal, flurbiprofen, ibuprofen, indomethacin, ketoprofen, ketorolac, meclofenamic acid, mefenamic acid, meloxicam, nabumetone, naproxen, nimesulide, nitroflurbiprofen, olsalazine, oxaprozin, phenylbutazone, piroxicam, sulfasalazine, sulindac, tolmetin or zomepirac;
• a barbiturate sedative, e.g. amobarbital, aprobarbital, butabarbital, butabital, mephobarbital, metharbital, methohexital, pentobarbital, phenobartital, secobarbital, talbutal, theamylal or thiopental;
• a benzodiazepine having a sedative action, e.g. chlordiazepoxide, clorazepate, diazepam, flurazepam, lorazepam, oxazepam, temazepam or triazolam;
• an Hi antagonist having a sedative action, e.g. diphenhydramine, pyrilamine, promethazine, chlorpheniramine or chlorcyclizine; • a sedative such as glutethimide, meprobamate, methaqualone or dichloralphenazone;
• a skeletal muscle relaxant, e.g. baclofen, carisoprodol, chlorzoxazone, cyclobenzaprine, methocarbamol or orphrenadine;
• an NMDA receptor antagonist, e.g. dextromethorphan ((+)-3-hydroxy-N-methylmorphinan) or its metabolite dextrorphan ((+)-3-hydroxy-N-methylmorphinan), ketamine, memantine, pyrroloquinoline quinine, cis-4-(phosphonomethyl)-2-piperidinecarboxylic acid, budipine, EN-3231 (MorphiDex®, a combination formulation of morphine and dextromethorphan), topiramate, neramexane or perzinfotel including an NR2B antagonist, e.g. ifenprodil, traxoprodil or (-)-(R)-6-{2-[4-(3-fluorophenyl)-4-hydroxy-1-piperidinyl]-1-hydroxyethyl-3,4-dihydr o-2(1 H)-quinoIinone;
• an alpha-adrenergic, e.g. doxazosin, tamsulosin, clonidine, guanfacine, dexmetatomidine, modafinil, or 4-amino-6,7-dimethoxy-2-(5-methane-sulfonamido-1 ,2,3,4-tetrahydroisoquinol-2- yl)-5-(2-pyridyl) quinazoline;
• a tricyclic antidepressant, e.g. desipramine, imipramine, amitriptyline or nortriptyline;
• an anticonvulsant, e.g. carbamazepine, lamotrigine, topiratmate or valproate;
• a tachykinin (NK) antagonist, particularly an NK-3, NK-2 or NK-1 antagonist, e.g. (αR,9R)-7-[3,5-bis(trifluoromethyl)benzyl]-8,9,10,11-tetrahydro-9-methyl-5-(4-met hylphenyl)-7H-[1 ,4]diazocino[2, 1 -g][1 ,7]-naphthyridine-6-13-dione (TAK-637), 5-[[(2R,3S)-2-[(1 R)-1-[3,5-bis(trifluoromethyl)phenyl]ethoxy-3-(4-fluorophenyl)-4- morpholinyl]-methyl]-1 ,2-dihydro-3H-1 ,2,4-triazol-3-one (MK-869), aprepitant, lanepitant, dapitant or 3-[[2-methoxy-5-(trifluoromethoxy)phenyl]-methylamino]-2-phenylpiperidine (2S,3S);
• a muscarinic antagonist, e.g oxybutynin, tolterodine, propiverine, tropsium chloride, darifenacin, solifenacin, temiverine and ipratropium;
• a COX-2 selective inhibitor, e.g. celecoxib, rofecoxib, parecoxib, valdecoxib, deracoxib, etoricoxib, or lumiracoxib;
• a coal-tar analgesic, in particular paracetamol; • a neuroleptic such as droperidol, chlorpromazine, haloperidol, perphenazine, thioridazine, mesoridazine, trifluoperazine, fluphenazine, clozapine, olanzapine, risperidone, ziprasidone, quetiapine, sertindole, aripiprazole, sonepiprazole, blonanserin, iloperidone, perospirone, raclopride, zotepine, bifeprunox, asenapine, lurasidone, amisulpride, balaperidone, palindore, eplivanserin, osanetant, rimonabant, meclinertant, Miraxion® or sarizotan;
• a vanilloid receptor agonist (e.g. resinferatoxin) or antagonist (e.g. capsazepine);
• a beta-adrenergic such as propranolol;
• a local anaesthetic such as mexiletine;
• a corticosteroid such as dexamethasone;
• a 5-HT receptor agonist or antagonist, particularly a 5-HT-|B/ID agonist such as eletriptan, sumatriptan, naratriptan, zolmitriptan or rizatriptan;
• a 5-HT2A receptor antagonist such as R(+)-alpha-(2,3-dimethoxy-phenyl)-1-[2-(4-fluorophenylethyl)]-4-piperidinemetha nol (MDL-100907);
• a cholinergic (nicotinic) analgesic, such as ispronicline (TC-1734), (E)-N-methyl-4-(3-pyridinyl)-3-buten-1 -amine (RJR-2403), (R)-5-(2-azetidinylmethoxy)-2-chloropyridine (ABT-594) or nicotine;
• Tramadol®;
• a PDEV inhibitor, such as 5-[2-ethoxy-5-(4-methyl-1 -piperazinyl-sulphonyl)phenyl]-1 -methyl-3-n-propyl-1 ,6- dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one (sildenafil), (6R,12aR)-2,3,6,7,12,12a-hexahydro-2-methyl-6-(3,4-methylenedioxyphenyl)-pyr azino[2',1':6,1]-pyrido[3,4-b]indole-1 ,4-dione (IC-351 or tadalafil), 2-[2-ethoxy-5-(4-ethyl-piperazin-1-yl-1-sulphonyl)-phenyl]-5-methyl-7-propyl-3H-i midazo[5,1 -f][1 ,2,4]triazin-4-one (vardenafil), 5-(5-acetyl-2-butoxy-3-pyridinyl)-3-ethyl-2-(1-ethyl-3-azetidinyl)-2,6-dihydro-7H-p yrazolo[4,3-αf|pyrimidin-7-one,
5-(5-acetyl-2-propoxy-3-pyridinyl)-3-ethyl-2-(1-isopropyl-3-azetidinyl)-2,6-dihydro- 7H-pyrazolo[4,3-α(]pyrimidin-7-one,
5-[2-ethoxy-5-(4-ethylpiperazin-1-ylsulphonyl)pyridin-3-yl]-3-ethyl-2-[2-methoxyet hyl]-2,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one,
4-[(3-chloro-4-methoxybenzyl)amino]-2-[(2S)-2-(hydroxymethyl)pyrrolidin-1 -yl]-N- (pyrimidin-2-ylmethyl)pyrimidine-5-carboxamide, 3-(1 -methyl-7-oxo-3-propyI-6,7-dihydro-1 H-pyrazolo[4,3-d]pyrimidin-5-yl)-N-[2-(1 - methylpyrrolidin-2-yl)ethyl]-4-propoxybenzenesulfonamide;
• an alpha-2-delta ligand such as gabapentin, pregabalin, 3-methylgabapentin, (1a,3a,5a)(3-amino-methyl-bicyclo[3.2.0]hept-3-yl)-acetic acid, (3S,5R)-3-aminomethyl-5-methyl-heptanoic acid, (3S,5R)-3-amino-5-methyl-heptanoic acid, (3S,5R)-3-amino-5-methyl-octanoic acid, (2S,4S)-4-(3-chlorophenoxy)proline, (2S,4S)-4-(3-fluorobenzyl)-proline, [(1 R,5R,6S)-6-(aminonnethyl)bicyclo[3.2.0]hept-6-yl]acetic acid, 3-(1 -aminomethyl-cyclohexylmethyl)-4H-[1 ,2,4]oxadiazol-5-one,
C-[1 -(1 H-tetrazol-δ-ylmethyO-cycloheptyll-methylamine,
(3S,4S)-(1 -aminomethyl-3,4-dimethyl-cyclopentyl)-acetic acid,
(SS.δRJ-S-aminomethyl-S-methyϊ-octanoic acid,
(3S,5R)-3-amino-5-methyl-nonanoic acid, (3S,5R)-3-amino-5-methyl-octanoic acid, (3R,4R,5R)-3-amino-4,5-dimethyl-heptanoic acid,
(3R,4R,5R)-3-amino-4,5-dimethyl-octanoic acid,
(2S)-2-Amino-4-ethyl-2-methylhexanoic acid and
(2S)-2-aminomethyl-5-ethyl-heptanoic acid;
• a cannabinoid;
• metabotropic glutamate subtype 1 receptor (mGluRI) antagonist;
• a serotonin reuptake inhibitor such as sertraline, sertraline metabolite demethylsertraline, fluoxetine, norfluoxetine (fluoxetine desmethyl metabolite), fluvoxamine, paroxetine, citalopram, citalopram metabolite desmethylcitalopram, escitalopram, d,l-fenfluramine, femoxetine, ifoxetine, cyanodothiepin, litoxetine, dapoxetine, nefazodone, cericlamine and trazodone;
• a noradrenaline (norepinephrine) reuptake inhibitor, such as maprotiline, lofepramine, mirtazepine, oxaprotiline, fezolamine, tomoxetine, mianserin, buproprion, buproprion metabolite hydroxybuproprion, nomifensine and viloxazine (Vivalan®), especially a selective noradrenaline reuptake inhibitor such as reboxetine, in particular (S,S)-reboxetine;
• a dual serotonin-noradrenaline reuptake inhibitor, such as venlafaxine, venlafaxine metabolite O-desmethylvenlafaxine, clomipramine, clomipramine metabolite desmethylclomipramine, duloxetine, milnacipran and imipramine;
• an inducible nitric oxide synthase (iNOS) inhibitor such as S-[2-[(1-iminoethyl)amino]ethyl]-L-homocysteine, S-[2-[(1-iminoethyl)-amino]ethyl]-4,4-dioxo-L-cysteine,
S-[2-[(1-iminoethyl)amino]ethyl]-2-nnethyl-L-cysteine,
(2S,5Z)-2-amino-2-methyl-7-[(1-iminoethyl)amino]-5-heptenoic acid,
2-[[(1 R,3S)-3-amino-4- hydroxy-1-(5-thiazolyI)-butyl]thio]-5-chloro-3-pyridinecarbonitrile; 2-[[(1 R,3S)-3-amino-4-hydroxy-1-(5-thiazolyl)butyl]thio]-4-chlorobenzonitrile, (2S,4R)-2-amino-4-[[2-chloro-5-(trifluoromethyl)phenyl]thio]-5-thiazolebutanoI, 2-[[(1 R,3S)-3-amino-4-hydroxy-1 -(5-thiazolyl) butyl]thio]-6-(trifluoromethyl)-3 pyridinecarbonitrile, 2-[[(1 R,3S)-3- amino-4-hydroxy- 1
-(5-thiazolyl)butyl]thio]-5-chlorobenzonitrile,
N-[4-[2-(3-chlorobenzylamino)ethyl]phenyl]thiophene-2-carboxamidine, or guanidinoethyldisulfide;
• an acetylcholinesterase inhibitor such as donepezil;
• a prostaglandin E2 subtype 4 (EP4) antagonist such as Λ/-[({2-[4-(2-ethyI-4,6-dimethyl-1 H-imidazo[4,5-c]pyridin-1-yl)phenyl]ethyI}amino)- carbonyl]-4-methylbenzenesulfonamide or 4-[(1 S)-1-({[5-chloro-2-(3-fluorophenoxy)pyridin-3-yl]carbonyl}amino)ethyl]benzoi c acid;
• a leukotriene B4 antagonist; such as 1-(3-biphenyl-4-ylmethyl-4-hydroxy-chroman-7-yl)-cyclopentanecarboxylic acid (CP-105696), 5-[2-(2-Carboxyethyl)-3-[6-(4-methoxyphenyl)-5E- hexenyl]oxyphenoxy]-valeric acid (ONO-4057) or DPC-11870,
• a 5-lipoxygenase inhibitor, such as zileuton, 6-[(3-fluoro-5-[4-methoxy-3,4,5,6-tetrahydro-2H-pyran-4-yl])phenoxy-methyl]-1-m ethyl-2-quinoIone (ZD-2138), or 2,3,5-trimethyl-6-(3-pyridylmethyl),1 ,4-benzoquinone (CV-6504);
• a sodium channel blocker, such as lidocaine;
• a 5-HT3 antagonist, such as ondansetron; and the pharmaceutically acceptable salts and solvates thereof.
In as much as it may desirable to administer a combination of active compounds, for example, for the purpose of treating a particular disease or condition, it is within the scope of the present invention that two or more pharmaceutical compositions, at least one of which contains a compound in accordance with the invention, may conveniently be combined in the form of a kit suitable for coadministration of the compositions. Thus the kit of the invention comprises two or more separate pharmaceutical compositions, at least one of which contains a compound of formula (I) in accordance with the invention, and means for separately retaining said compositions, such as a container, divided bottle, or divided foil packet. An example of such a kit is the familiar blister pack used for the packaging of tablets, capsules and the like.
The kit of the invention is particularly suitable for administering different dosage forms, for example, oral and parenteral, for administering the separate compositions at different dosage intervals, or for titrating the separate compositions against one another. To assist compliance, the kit typically comprises directions for administration and may be provided with a so-called memory aid. Examples
The invention is illustrated in the following non-limiting examples in which, unless stated otherwise: all operations were carried out at room or ambient temperature, that is, in the range of 18-25 0C; evaporation of solvent was carried out using a rotary evaporator under reduced pressure with a bath temperature of up to 60 0C; reactions were monitored by thin layer chromatography (TLC) and reaction times were given for illustration only; melting points (mp) given were uncorrected (polymorphism may result in different melting points); the structure and purity of all isolated compounds were assured by at least one of the following techniques: TLC (Merck silica gel 60 F2S4 precoated TLC plates), mass spectrometry, nuclear magnetic resonance spectra (NMR), infrared red absorption spectra (IR) or microanalysis. Yields were given for illustrative purposes only. Flash column chromatography was carried out using Merck silica gel 60 (230-400 mesh ASTM) or Fuji Silysia amino bounded silica (Chromatorex, 30-50 uM) or Biotage amino bounded silica (35-75 Dm, KP-NH) or Biotage silica (32-63 Dm, KP-SiI). The purification using HPLC was perfomed by the following apparatus and conditions. Apparatus : UV-trigger preparative HPLC system, Waters (Column: XTerra MS C18, 5 urn, 19 x 50 mm or 30 x 50 mm), Detector: UV 254 nm Conditions : CH3CN/0.05% HCOOH aqueous solution or CH3CN/0.01% NH3 aqueous solution; 20ml/min (19 x 50 mm) or 40ml/min (30 x 50 mm) at ambient temperature. Microwave apparatus used in the reaction was Emrys optimizer (Personal chemistry). Optical rotation was measured by P-1020 (Jasco). Low-resolution mass spectral data (El) were obtained on a Integrity (Waters) mass spectrometer. Low-resolution mass spectral data (ESI) were obtained on a ZMD (Micromass) mass spectrometer. NMR data were determined at 270 MHz (JEOL JNMLA 270 spectrometer) or 300 MHz (JEOL JNMLA300 spectrometer) using deuteratθd chloroform (99.8% D) or DMSO (99.9% D) as solvent unless indicated otherwise, relative to tetramethylsilane (TMS) as internal standard in parts per million (ppm); conventional abbreviations used were: s = singlet, d = doublet, t = triplet, q = quartet, quint = quintet, m = multiplet, br. = broad, etc. IR spectra were measured by a Shimazu infrared spectrometer (IR-470). Chemical symbols have their usual meanings; bp (boiling point), mp (melting point), L (liter(s)), ml (milliliter(s)), g (gram(s)), mg (milligram(s)), mol (moles), mmol (millimoles), eq. (equivalent(s)), quant, (quantitative yield), sat. (saturated), aq (aqua). In the following Examples, "Me" means methyl and "Et" means ethyl. Preparations
The following Preparations illustrate the preparation of certain Amine and Carboxylic Acid intermediates used to prepare the Examples herein below. Amine 1 : 1-Quinolin-4-ylethanamine. dihvdrochloride
Figure imgf000046_0001
1-Quinolin-4-ylethanamine dihydrochloride can be prepared by the method described in Journal of the Chemical Society, Perkin Transactions 2, (1999), (11), pp 2415-2418. Amine 2: 1-lsoquinolin-5-ylmethanamine hydrochloride
Figure imgf000046_0002
1-lsoquinolin-5-ylmethanamine was synthesized as described in WO 2001070229. Amine 3: 1-isoαuinolin-8-ylmethanamine
Figure imgf000046_0003
1-lsoquinolin-8-ylmethanamine was synthesized as described in WO 2001070229. Amine 4: 1-(6-Fluoroαuinolin-4-vDmethanamine dihvdrochloride
Figure imgf000047_0001
Step 4-A) 6-fluoroquinoline-4-carbonitrile
A mixture of 4-chloro-6-fluoroquinoline(APOLLO) (1120 mg, 6.17 mmol), zinc cyanide
(1450 mg, 12.3 mmol) and palladium (0) tetrakis(triphenylphosphine)(713 mg, 0.617 mmol) in dry DMF (15 ml) was treated with microwave conditions (1600C, 30 min). The mixture was diluted with ethyl acetate and filtered through a pad of celite. To the filtrate was added toluene (ca.20ml) and the organic layer was washed with water (x2), brine, dried and concentrated in vacuo to give crude product. The crude product was purified by column chromatography on silica gel (ca. 250 g), eluting with hexane -ethyl acetate
(3:1), to give the title compound ( 880 mg, white solid).
1H NMR (270 MHz, CDCI3) δ 7.60-7.70 (1 H, m), 7.75-7.79 (1 H, m), 7.81-7.87 (1 H, m),
8.20 - 8.28 (1 H, m), 9.00-9.05 (1 H, m).
Step 4-B) 1-(6-fluoroquinolin-4-yl)methanamine dihvdrochloride
A solution of the product of step 4-A (880 mg, 5.11 mmol) in 10% hydrochloric methanol
(10 ml) and methanol (30 ml) was hydrogenated over 20% palladium hydroxide (150 mg) under balloon pressure (room temperature) for 6 hours. The mixture was diluted with methanol and the catalyst was filtered through a pad of celite pad (the filter cake was washed with methanol). The filtrate and washings were evaporated in vacuo to give crude product as white solid, which was recrystallized from methanol -diisopropyl ether to give the title compound (376 mg, slightly yellow solid).
1H NMR (270 MHz, DMSO-αfe) δ 4.62-4.73 (2H, m), 7.86-7.967 (2H, m), 8.14-8.22 (1 H, m), 8.30-8.39 (1 H, m), 9.02 (2H, br,s), 9.12-9.17 (1 H, m). MS (ESI) m/z 177 (M + H)+.
Amine 5: 1-(6,8-Difluoroquinolin-4-yl)methanamine dihvdrochloride
. 2HCl
Figure imgf000047_0002
Step 5-A: 6.8-difluoroquinoline-4-carbonitrile
A mixture of 4-chloro-6,8-difIuoroquinoIine(APOLLO) (1000 mg, 5.01 mmol), zinc cyanide (1180 mg, 10.0 mmol) and palladium (0) tetrakis(triphenylphosphine)(579 mg, 0.501 mmol) in dry DMF (15 ml) was treated in the same procedure as described in Step 4-A. The crude product was purified by column chromatography on silica gel (ca. 250 g), eluting with hexane -ethyl acetate (3:1), to give the title compound ( 787 mg, yellow solid). 1H NMR (270 MHz, CDCI3) δ 7.37-7.50 (1 H, m), 7.65-7.75 (1 H, m), 7.83-7.90 (1 H, m),
9.05 - 9.10 (1 H, m).
Step 5-B: 1-(6,8-difluoroquinolin-4-v0methanamine dihvdrochloride A solution of the product of step 5-A (787 mg, 4.14 mmol) in 10% hydrochloric methanol (10 ml) and methanol (30 ml) was treated in the same procedure as described in Step 4-B. The filtrate and washings were evaporated in vacuo to give crude product as a white solid, which was recrystallized from methanol -diisopropyl ether to give the title compound (1030 mg, slightly yellow solid). 1H NMR (270 MHz, CDCI3) δ 4.51-4.62 (m, 2H), 7.78-7.97 (3H, m), 8.85-9.05 (3H, m). MS (ESI) m/z 195 (M + H)+. Amine 6: 1-quinolin-4-ylmethanamine
Figure imgf000048_0001
1 -Quinolin-4-ylmethanamine can be prepared by the method described in Yakugaku
Zasshi (1952), 72, pp 167-172.
Carboxylic acid 1 : 6-(trifluoromethvO-2-naphthoic acid
Figure imgf000048_0002
Carboxylic acid 1 was synthesized following the procedure described in Synthesis (2005), (5), pp 791-797. Carboxvlic acid 2: 6-(trifluoromethv0quinoline-2-carboxvlic acid
Figure imgf000049_0001
Carboxylic acid 2 was synthesized following the procedure described in WO
2005033082 A2.
Carboxylic acid 3: 7-(trifluoromethyl)quinoline-3-carboxylic acid
Figure imgf000049_0002
Carboxylic acid 3 was synthesized following the procedure described in Journal of
Medicinal Chemistry (1979), 22(7), pp 816-823.
Carboxylic acid 4: 6-Fluoro-7-(trifluoromethyl)quinoline-3-carboxylic acid
Figure imgf000049_0003
Step 4-1 : Ethyl 6-fluoro-4-hvdroxy-7-(trifluoromethyl)quinoline-3-carboxylate
A mixture of 3-trifluoromethyl-4-fluoroaniline (15 g, 84 mmol, purchased from Wako) and diethylethoxymethylene malonate (22.8 ml_, 113 mmol) was heated slowly as follows; 60
°C for 10 minutes, 90 0C for 15 minutes, 140 °C for 90 minutes. After EtOH was removed in vacuo, the residue was solidified upon standing. This solid was added portionwise to boiling diphenyl ether (278 ml_) at 250 0C, and the resulting dark yellow solution was stirred at this temperature for 90 minutes. After being cooled to room temperature, the white solid began to precipitate out. This solid material was filtered, and washed with ethyl acetate-hexanes 2:1 (ca. 500 mL) to give the crude title compound as a white solid
(3.38 g, 13 % yield). This crude product was used for the next step without further purification. 1H NMR (270 MHz, DMSO-d6) δ ppm 1.30 (3H, t, J= 7.3 Hz), 4.25 (2H, q,
J= 7.3 Hz), 7.98-8.16 (1 H, m), 8.73 (1 H, s), 12.59 (1 H, br s). MS (ESI) m/z 302 (M -H)",
304 (M + H)+.
Step 4-2: Ethyl 4-chloro-6-fluoro-7-(trifluoromethvDquinoline-3-carboxylate
A mixture of the product of Step 4-1 (3.38 g, 11.1 mmol) and phosphorous oxychloride (20.8 mL, 223 mmol) was heated under reflux for 120 minutes. After phosphorous oxychloride was removed in vacuo, the residue was diluted in dichloromethane and poured into crushed ice-25 % ammonia water mixture portionwise. The aqueous layer was extracted with dichloromethane 3 times. The combined organic extracts were washed with brine, dried over sodium sulfate and concentrated. The residue was chromatographed on a column of silica gel, eluting with ethyl acetate-hexane (1 :5), to give the title compound (3.38 g, 94 %) as a white solid. 1H NMR (270 MHz, CDCi3) δ ppm 1.48 (3H, t, J= 7.3 Hz), 4.54 (2H, q, J= 7.3 Hz), 8.19 (1 H, d, J= 11.2 Hz), 8.49 (1 H, d, J= 6.6 Hz), 8.25 (1 H, s). MS (ESI) m/z 322 (M + H)+. Step 4-3: Ethyl 6-fluoro-7-(trifluoromethyl)quinoline-3-carboxylate A mixture of the product of Step 4-2 (3.38 g, 10.5 mmol), 5 % palladium on activated carbon (338 mg), triethylamine (2.93 mL, 21.0 mmol) and ethanol (50 mL) was hydrogenated (1 atm, balloon) at room temperature for 90 minutes. The reaction mixture was filtered over a pad of celite, and the filtrate was evaporated. The residue was chromatographed on a column of silica gel, eluting with ethyl acetate-hexane (1 :10 to 1 :5), to give the title compound (2.94 g, 97 %) as yellow solid. 1H NMR (270 MHz, CDCI3) δ ppm 1.48 (3H, t, J= 7.3 Hz), 4.51 (2H, q, J= 7.3 Hz), 7.72 (1 H, d, J= 9.9 Hz), 8.51 (1 H, d, J= 7.3 Hz), 8.84 (1 H, br s), 9.51 (1 H, br s). MS (ESI) m/z 288 (M + H)+. Step 4-4: δ-Fluoro^-ftrifluoromethvDquinoline-S-carboxylic acid To a solution of the product of Step 4-3 (2.94 g, 10.2 mmol) in ethanol (4 ml) was added 2N aqueous sodium hydroxide (10.2 mL, 20.5 mmol) at room temperature. The mixture was stirred at 60 0C for 2 hours. Then the reaction mixture was neutralized to pH 5-6 by 2N aqueous hydrochloride. The formed precipitate was collected and washed with water to furnish the title compound (2.52 g, 95 %) as a white solid. 1H NMR (270MHz, DMSO-Qf6) δ ppm 8.32 (1 H, d, J = 11.2 Hz), 8.51 (1 H, d, J = 7.3 Hz), 9.01 (1 H, s), 9.42 (1 H, s). MS (ESI) : m/z 260 (M + H)+ 258 (M - H)+.
Carboxylic acid 5: β-Chloro^-ftrifluoromethvOαuinoline-S-carboxylic acid
Figure imgf000050_0001
Step 5-1 : Ethyl 6-chloro-4-hvdroxy-7-(trifluoromethvπαuinoline-3-carboxylate
The title compound was prepared by the procedure described in Step 4-1 using
3-trifluoromethyl-4-chloroaniline instead of 3-trifluoromethyl-4-fluoroaniline. 1H NMR (270 MHz, DMSO-Gf6) δ ppm 1.30 (3H, t, J= 7.3 Hz), 4.25 (2H, q, J= 7.3 Hz),
8.16 (1 H, s), 8.73 (1 H, s), 12.60 (1 H1 br s). MS (ESI) m/z 318 (M -H)-, 320 (M + H)+.
Step 5-2: Ethyl 4-bromo-6-chloro-7-(trifluoromethyl)quinoline-3-carboxylate
A mixture of the product of Step 5-1 (2.00 g, 6.26 mmol), phosphorous oxybromide (5.38 g, 18.8 mmol) and N,N-dimethylformamide (40 ml_) was stirred at 70 0C for 2 hours. After phosphorous oxychloride was removed in vacuo, the residue was diluted in dichloromethane and poured onto crushed ice carefully. The mixture was diluted with saturated aqueous sodium bicarbonate (300 ml_) and extracted with dichloromethane 3 times (total 150 ml_). The combined organic extracts were dried over sodium sulfate and concentrated. The residue was chromatographed on a column of silica gel, eluting with ethyi acetate-hexane (1 :5), to give the title compound (2.15 g, 90 %) as a white solid.
1H NMR (270 MHz, CDCI3) δ ppm 1.48 (3H, t, J= 7.3 Hz), 4.54 (2H, q, J= 7.3 Hz), 8.53
(1 H, s), 8.56(1 H, s), 9.14 (1 H, s). MS (ESI) m/z 384, 382 (M + H)+.
Step 5-3: Ethyl 6-chloro-7-(trifluoromethyl)quinoline-3-carboxylate
A mixture of the product of step 5-2 (2.15 g, 5.63 mmol), 5 % palladium on activated carbon (215 mg), triethylamine (1.57 ml_, 11.30 mmol) and ethanol (56 ml_) was hydrogenated (1 atm, balloon) at room temperature for 55 minutes. The reaction mixture was filtered over a pad of celite, and the filtrate was evaporated. The residue was chromatographed on a column of silica gel, eluting with ethyl acetate-hexane (1 :10 to
1 :5), to give a mixture of the title compound and ethyl
7-(trifluoromethyl)quinoline-3-carboxylate (des-diCI derivative). The mixture was recrystallized from hexane (50 ml_) to give the title compound (0.61 g, 35 %) as a white solid. 1H NMR (270 MHz, CDCI3) δ ppm 1.48 (3H, t, J= 7.3 Hz), 4.52 (2H, q, J= 7.3 Hz),
8.10 (1 H, s), 8.57 (1 H, s), 8.81 (1 H, d, J= 2.0 Hz), 9.53 (1 H, d, J= 2.0 Hz). MS (ESI) m/z 304 (M + H)+.
Step 5-4: e-Chloro^-ftrifluoromethvDquinoline-S-carboxylic acid
The title carboxylic acid was prepared by the procedure described in step 4-4 to give the title compound (595 mg, quant) as a white solid. 1H NMR (270MHz, DMSO-CZ6) δ ppm
8.57 (1 H, s), 8.67 (1 H, s), 9.09 (1 H, d, J= 2.0 Hz), 9.44 (1 H, d, J= 2.0 Hz). MS (ESI) : m/z 276 (M + H)+ 274 (M - H)+.
Examples
Example 1 : Λ/-πsoquinolin-5-ylmethyl)-6-(trifluoromethvO-2-naphthamide
Figure imgf000052_0001
To a DMF (7 ml) solution of Amine 2 (53 mg, 0.23 mmol) were added Carboxylic Acid 1 (50 mg, 0.210 mmol), HBTU (118 mg, 0.31 mmol) and trimethylamine (105 mg, 1.04 mmol) and the mixture was stirred for 3 hours at room temperature. The reaction was quenched with water and the product was extracted with EtOAc. Then, evaporation and purification gave a brown oil which was further purified by silica gel column chromatography, eluting with hexane-ethyl acetate (1 :2), to afford the title compound
(53.5 mg, 67.6%) as a white solid. 1H-NMR (270 MHz, DMSO-Of6) δ 5.02 (2H, d, J=5.3 Hz), 7.72-7.66 (1 H, m), 7.85-7.81 (2H1 m), 8.14-8.07 (3H, m), 8.29-8.23 (2H, m), 8.63-8.50 (3H, m), 9.41-9.36 (2H, m). MS (ESI) : m/z 379 (M - H)", 381 (M + H)+.
Examples 2 to 11 were prepared according to the process disclosed in example 1 , using the appropriate starting material. Example 2 : Λ/-(Quinolin-4-ylmethyl)-6-(trifluoromethyl)-2-naphthamide
Figure imgf000052_0002
Example 2 was prepared from Amine 1 and Carboxylic Acid 1.
1H NMR (270 MHz, DMSO-cfe) δ 5.05-5.11 (2H, m),"7.48-7.53 (1 H, m), 7.64-7.89 (3H, m),
8.05-8.20 (2H, m), 8.24-8.34 (3H, m), 8.52 (1 H, br,s), 8.68 (1 H, br,s), 8.86-8.91 (m, 1 H),
9.44-9.53 (1 H, m). MS (ESI) m/z 379 (M - H)-, 381 (M + H)+
Example 3 : Λ/-(lsoquinolin-5-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide
Figure imgf000052_0003
Example 3 was prepared from Amine 2 and Carboxylic Acid 3. H-NMR (270 MHz, DMSO-d6) δ 5.04 (2H, d, J=5.3 Hz), 7.70 (1 H, t, J=7.6 Hz), 7.86 (1 H, d, J=6.6 Hz), 8.14-8.08 (3H, m), 8.30 (1 H1 d, J=8.6 Hz), 8.59 (1 H3 d, J=5.9 Hz), 8.64 (1 H, s), 9.08 (1 H, d, J=2.0 Hz), 9.37 (1 H, s), 9.48 (1 H, d, J=2.0 Hz), 9.54 (1 H, t, J=5.6 Hz). MS (ESI) : m/z 380 (M - H)-, 382 (M + H)+. Example 4: ΛK1-Quinolin-4-ylethvπ-7-ftrifluoromethyl)quinoline-3-carboxamide
Figure imgf000053_0001
Example 4 was prepared from Amine 1 and Carboxylic Acid 3.
1H-NMR (270 MHz, DMSO-Cf6) δ 1.68 (3H, d, J=7.3 Hz), 6.07-5.97 (1 H, m), 7.66(1 H, d, J=4.6 Hz), 7.71 (1 H1 d, J=7.0 Hz)1 7.80 (1 H1 1, J=8.2 Hz)1 7.99 (1 H1 dd, J=8.6, 2.0 Hz)1 8.08 (1 H1 d, J=7.9 Hz)1 8.34 (1 H1 d, J=7.9 Hz), 8.40 (1 H1 d, J=8.6 Hz)1 8.47 (1 H1 s), 8.90 (1 H1 d, J=4.6 Hz), 9.05 (1 H1 d, J=2.0 Hz), 9.45 (1 H, d, J=2.6 Hz), 9.57 (1 H1 d, J=7.3 Hz). MS (ESI) : m/z 394 (M - H)", 396 (M + H)+. Example s: AKlsoquinolin-8-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide
Figure imgf000053_0002
Example 5 was prepared from Amine 3 and Carboxylic Acid 3
LC-MS: Retention time: 2.28 min. MS (ESI) m/z 382 (M + H)+
HPLC condition: Waters (Column: Xterra PrepMS C18, 3.5um, 4.6x50mm), Detector: photodiodearray (210- 400 nm) Conditions: water / MeOH / 1% NH3 aqueous solution;
2.0 ml/min at 400C.
Example 6: Λ/-(lsoquinolin-5-ylmethyl)-6-(trifluoromethyl)quinoline-2-carboxamide
Figure imgf000053_0003
Example 6 was prepared from Amine 2 and Carboxylic Acid 2
1H-NMR (270 MHz, DMSO-c/6) δ 5.04 (2H1 d, J=5.9 Hz), 7.68 (1 H, t, J=7.6 Hz), 7.82 (1 H, d, J=6.6 Hz), 8.13-8.06 (2H, m), 8.19 (1 H, d, J=5.9 Hz), 8.35-8.31 (2H1 m), 8.58 (1 H, d,
J=5.9 Hz), 8.66 (1 H, s), 8.81 (1 H, d, J=8.6 Hz), 9.35 (1 H, s), 9.70 (1 H, t, J=6.3 Hz). MS
(ESI) : m/z 380 (M - H)", 382 (M + H)+.
Example 7: Λ/-(Quinolin-4-ylmethyl)-6-(trifluoromethvπαuinoline-2-carboxamide
Figure imgf000054_0001
Example 7 was prepared from Amine 1 and Carboxylic Acid 2
1H-NMR(DMSO-CZ6, 270 MHz) δ 5.11 (2H, d, J= 5.9 Hz), 7.47 (1 H, d, J= 4.6 Hz), 7.69 (1 H, t, J= 7.9 Hz), 7.81 (1 H, t, J= 7.9 Hz), 8.08 (1 H1 d, J= 7.9 Hz), 8.15 (1 H, d, J= 7.3 Hz),
8.29-8.42 (3H, m), 8.68 (1 H, s), 8.78-8.90 (2H, m), 9.80 (1 H1 t, J= 6.6 Hz). MS(ESI): m/z 382 (M+H)+, 380 (M-H)+.
Example 8:
6-Fluoro-Λ/-(isoquinolin-5-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide
Figure imgf000054_0002
Example 8 was prepared from Amine 2 and Carboxylic Acid 4
1H-NMR (270 MHz, DMSO-Qf6) δ 5.03 (2H, d, J=5.3 Hz), 7.69 (1 H, t, J=7.6 Hz), 7.85 (1 H, d, J=6.6 Hz), 8.08 (1 H, s), 8.11 (1 H, s), 8.27 (1 H, d, J=11.2 Hz), 8.54 (1 H, d, J=7.3 Hz), 8.59 (1 H, d,J=5.9 Hz), 8.96 (1 H, d, J=2.0 Hz), 9.36 (1 H, s), 9.43 (1 H, d, J=2.6 Hz), 9.61 (1 H, t, J=5.9 Hz). MS (ESI): m/z 398 (M - H)", 400 (M + H)+. Example 9: 6-Chloro-Λ/-πsoquinolιn-5-vlmethvπ-7-(trifluoromethvπquinoline-3-carboxamide
Figure imgf000055_0001
Example 9 was prepared from Amine 2 and Carboxylic Acid 5.
1H-NMR (270 MHz, DMSO-ofe) δ 5.03 (2H1 d, J=5.3 Hz), 7.69 (1 H, t, J=7.6 Hz), 7.85 (1 H, d, J=6.7 Hz), 8.11-8.08 (2H, m), 8.60-8.57 (3H, m), 8.95 (1 H, d, J=2.0 Hz), 9.36 (1 H, s), 9.47 (1 H, d, J=2.6 Hz), 9.61 (1 H, t, J=5.6 Hz). MS (ESI): m/z 414 (M - H)", 416 (M + H)+. Example 10: 6-Fluoro-N-(quinolin-4-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide
Figure imgf000055_0002
Example 10 was prepared from Amine 6 and Carboxylic Acid 4.
1H-NMR(DMSO-CZ6, 270 MHz) δ 5.10 (2H, d, J= 5.3 Hz), 7.55 (1 H, d, J= 4.0 Hz), 7.70 (1 H, t, J= 7.3 Hz), 7.82 (1 H, t, J= 8.6 Hz), 8.09 (1 H, d, J= 7.9 Hz), 8.28 (1 H, s), 8.31 (1 H, d, J=
4.0 Hz), 8.57 (1 H, d, J= 6.6 Hz), 8.88 (1 H, d, J= 4.0 Hz), 9.01 (1 H, br s), 9.47 (1 H, br s),
9.71 (1 H, t, J= 5.9 Hz). MS(ESI): positive 400 (M+H)+, negative 398 (M-H)+.
Example 11 :
6-Ch loro-N-(q u i nol i n-4-yl meth yl)-7-(trif I uorometh yl)q u i nol i ne-3-carboxam ide
Figure imgf000055_0003
Example 11 was prepared from Amine 6 and Carboxylic Acid 5.
1H-NMR(DMSO-Of6, 270 MHz) δ 5.10 (2H, d, J= 5.9 Hz), 7.55 (1 H, d, J= 4.0 Hz), 7.70 (1 H, t, J= 6.6 Hz), 7.82 (1 H, t, J= 6.6 Hz), 8.09 (1 H, d, J= 7.9 Hz), 8.29 (1 H, d, J= 7.9 Hz), 8.59 (2H, s), 8.89 (1 H, d, J= 4.6 Hz), 9.01 (1 H, d, J= 2.0 Hz), 9.51 (1 H, d, J= 2.0 Hz), 9.71 (1 H, t, J= 5.3 Hz). MS(ESI): positive 416 (M+H)+, negative 414 (M-H)+

Claims

1. A compound of the formula (I):
Figure imgf000056_0001
wherein, one of X1, X2 and X3 is N, and the others are CH;
X4 is N and X5 is CH, or X4 and X5 are CH, or X4 is CH and X5 is N;
R1 is hydrogen or CrC4 alkyl;
R2 is hydrogen or halogen; or a pharmaceutically acceptable salt or solvate thereof.
2. A compound of formula (I), according to claim 1 , or a pharmaceutically acceptable salt or solvate thereof, wherein R1 is CrC4 alkyl and the compound is of the formula (Ia):
Figure imgf000056_0002
3. A compound according to claim 1or 2, or a pharmaceutically acceptable salt or solvate thereof, where R1 is hydrogen, methyl or ethyl.
4. A compound according to any one of the preceding claims, or a pharmaceutically acceptable salt or solvate thereof, where R2 is hydrogen, fluoro or chloro.
5. A compound according to claim 1 , or a pharmaceutically acceptable salt or solvate thereof, wherein the compound is selected from the group consisting of: Λ/-(isoquinolin-5-ylmethyl)-6-(trifluoromethyl)-2-naphthamide, /V-(quinolin-4-ylmethyl)-6-(trifluoromethyl)-2-naphthamide, Λ/-(isoquinolin-5-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide, Λ/-(1-quinolin-4-ylethyl)-7-(trifluoromethyl)quinoline-3-carboxamide, Λ/-(isoquinolin-8-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide, Λ/-(isoquinolin-5-ylmethyl)-6-(trifluoromethyl)quinoline-2-carboxamide, Λ/-(quinolin-4-ylmethyl)-6-(trifluoromethyl)quinoline-2-carbόxamide, 6-fluoro-Λ/-(isoquinolin-5-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide, 6-chloro-Λ/-(isoquinolin-5-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide, 6-fluoro-N-(quinolin-4-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide, and
6-chloro-N-(quinolin-4-ylmethyl)-7-(trifluoromethyl)quinoline-3-carboxamide.
6. A pharmaceutical composition including a compound of the formula (I) or a pharmaceutically acceptable salt or solvate thereof, as defined in any one of claims 1 to 5, together with a pharmaceutically acceptable excipient.
7. A compound of formula (I), or a pharmaceutically acceptable salt or solvate or composition thereof, as defined in any one of claims 1 to 6, for use as a medicament.
8. Use of a compound of the formula (I), or a pharmaceutically acceptable salt or solvate or composition thereof, as defined in any one of claims 1 to 6, for the manufacture of a medicament for the treatment of a disease for which a VR1 antagonist is indicated.
9. A use according to claim 8, wherein the disease is selected from pain, including chronic pain, acute pain, nociceptive pain, neuropathic pain, inflammatory pain, post herpetic neuralgia, neuropathies, neuralgia, diabetic neuropathy, HIV-related neuropathy, nerve injury, rheumatoid arthritic pain, osteoarthritic pain, burns, back pain, visceral pain, cancer pain, dental pain, headache, migraine, carpal tunnel syndrome, fibromyalgia, neuritis, sciatica, pelvic hypersensitivity, pelvic pain and menstrual pain; bladder disease, such as urinary incontinence, lower urinary tract symptoms, micturition disorder, renal colic and cystitis; inflammation, such as burns, rheumatoid arthritis and osteoarthritis; neurodegenerative disease, such as stroke, post stroke pain and multiple sclerosis; diseases of the respiratory tree that have a contribution to symptons or pathology arising from the sensory afferent nervous system, such as cough, bronchoconstriction, irritation, inflammation and other pathways in diseases of the lower airway such as asthma and COPD as well as those of the upper airway, such as allergic rhinitis and chronic sinusitis; gastrointestinal disorders, such as gastroesophageal reflux disease (GERD), dysphagia, ulcer, irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), colitis and Crohn's disease; ischemia, such as cerebrovascular ischemia and acute cerebral ischemia; emesis, such as cancer chemotherapy-induced emesis; diabetes and obesity.
10. A method of treating a disease for which a VR1 antagonist is indicated, in a mammal, including a human being, including treating said mammal with an effective amount of a compound of the formula (I), or with a pharmaceutically acceptable salt, solvate or composition thereof, as defined in any one of claims 1 to 6.
11. A combination of a compound of the formula (I) or a pharmaceutical acceptable salt or solvate thereof, as defined in any one of claims 1 to 5, and another pharmacologically active agent.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009089057A1 (en) * 2008-01-09 2009-07-16 Renovis, Ins. Amide derivatives as ion-channel ligands and pharmaceutical compositions and methods of using the same
WO2012177668A1 (en) 2011-06-20 2012-12-27 E. I. Du Pont De Nemours And Company Heterocyclic compounds for treating helminth infections
WO2013081783A1 (en) 2011-11-28 2013-06-06 E. I. Du Pont De Nemours And Company N- (4 -quinolinylmethyl) sulfonamide derivatives and their use as anthelmintics
WO2014099837A1 (en) 2012-12-18 2014-06-26 E. I. Du Pont De Nemours And Company Sulfonamide anthelmintics
US10656141B2 (en) 2014-01-23 2020-05-19 Biogaia Ab Selection of agents modulating gastrointestinal pain

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WO2004069792A2 (en) * 2003-02-03 2004-08-19 Janssen Pharmaceutica N.V. Quinoline-derived amide modulators of vanilloid vr1 receptor

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WO2004069792A2 (en) * 2003-02-03 2004-08-19 Janssen Pharmaceutica N.V. Quinoline-derived amide modulators of vanilloid vr1 receptor

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009089057A1 (en) * 2008-01-09 2009-07-16 Renovis, Ins. Amide derivatives as ion-channel ligands and pharmaceutical compositions and methods of using the same
US8163901B2 (en) 2008-01-09 2012-04-24 Evotec Ag Amide derivatives as ion-channel ligands and pharmaceutical compositions and methods of using the same
WO2012177668A1 (en) 2011-06-20 2012-12-27 E. I. Du Pont De Nemours And Company Heterocyclic compounds for treating helminth infections
WO2013081783A1 (en) 2011-11-28 2013-06-06 E. I. Du Pont De Nemours And Company N- (4 -quinolinylmethyl) sulfonamide derivatives and their use as anthelmintics
WO2014099837A1 (en) 2012-12-18 2014-06-26 E. I. Du Pont De Nemours And Company Sulfonamide anthelmintics
US10656141B2 (en) 2014-01-23 2020-05-19 Biogaia Ab Selection of agents modulating gastrointestinal pain
US11371985B2 (en) 2014-01-23 2022-06-28 Biogaia Ab Selection of agents modulating gastrointestinal pain

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