WO2007015697A3 - Method for enhancing two-dimensional gel electrophoresis resolution - Google Patents

Method for enhancing two-dimensional gel electrophoresis resolution Download PDF

Info

Publication number
WO2007015697A3
WO2007015697A3 PCT/US2005/029107 US2005029107W WO2007015697A3 WO 2007015697 A3 WO2007015697 A3 WO 2007015697A3 US 2005029107 W US2005029107 W US 2005029107W WO 2007015697 A3 WO2007015697 A3 WO 2007015697A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
enhancing
gel electrophoresis
dimensional gel
resolution
Prior art date
Application number
PCT/US2005/029107
Other languages
French (fr)
Other versions
WO2007015697A9 (en
WO2007015697A2 (en
Inventor
Fengju Bai
Sheng Liu
Frank A Witzmann
Original Assignee
Univ Indiana Res & Tech Corp
Fengju Bai
Sheng Liu
Frank A Witzmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Indiana Res & Tech Corp, Fengju Bai, Sheng Liu, Frank A Witzmann filed Critical Univ Indiana Res & Tech Corp
Publication of WO2007015697A2 publication Critical patent/WO2007015697A2/en
Publication of WO2007015697A9 publication Critical patent/WO2007015697A9/en
Publication of WO2007015697A3 publication Critical patent/WO2007015697A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

One aspect of the present disclosure is directed to compositions and methods to decrease streaking on two-dimensional gels and enhance the resolution of proteins in the gel. In accordance with one embodiment the polypeptides are contacted with a trialkylphosphine to reduce the polypeptides, followed by alkylation with a compound that provides greater than 95% alkylation of the polypeptides. The resulting alkylated polypeptides are then subjected to two-dimensional gel analysis.
PCT/US2005/029107 2004-08-16 2005-08-16 Method for enhancing two-dimensional gel electrophoresis resolution WO2007015697A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60179104P 2004-08-16 2004-08-16
US60/601,791 2004-08-16

Publications (3)

Publication Number Publication Date
WO2007015697A2 WO2007015697A2 (en) 2007-02-08
WO2007015697A9 WO2007015697A9 (en) 2007-04-19
WO2007015697A3 true WO2007015697A3 (en) 2007-06-21

Family

ID=37709025

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/029107 WO2007015697A2 (en) 2004-08-16 2005-08-16 Method for enhancing two-dimensional gel electrophoresis resolution

Country Status (1)

Country Link
WO (1) WO2007015697A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201512012D0 (en) * 2015-07-09 2015-08-19 Ge Healthcare Bio Sciences Ab Method for analyzing a sample

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030221963A1 (en) * 2002-05-31 2003-12-04 Bengt Bjellqvist Method of electrophoresis
US20040108209A1 (en) * 1996-12-05 2004-06-10 Macquarie Research Ltd. Electrophoresis separation methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040108209A1 (en) * 1996-12-05 2004-06-10 Macquarie Research Ltd. Electrophoresis separation methods
US20030221963A1 (en) * 2002-05-31 2003-12-04 Bengt Bjellqvist Method of electrophoresis

Also Published As

Publication number Publication date
WO2007015697A9 (en) 2007-04-19
WO2007015697A2 (en) 2007-02-08

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