WO2002090992A3 - Screening method - Google Patents

Screening method Download PDF

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Publication number
WO2002090992A3
WO2002090992A3 PCT/GB2002/001946 GB0201946W WO02090992A3 WO 2002090992 A3 WO2002090992 A3 WO 2002090992A3 GB 0201946 W GB0201946 W GB 0201946W WO 02090992 A3 WO02090992 A3 WO 02090992A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell array
agents
trap
genes
identification
Prior art date
Application number
PCT/GB2002/001946
Other languages
French (fr)
Other versions
WO2002090992A2 (en
Inventor
Peter Andrews
Jon Draper
James Walsh
Original Assignee
Axordia Ltd
Peter Andrews
Jon Draper
James Walsh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axordia Ltd, Peter Andrews, Jon Draper, James Walsh filed Critical Axordia Ltd
Priority to AU2002249467A priority Critical patent/AU2002249467A1/en
Publication of WO2002090992A2 publication Critical patent/WO2002090992A2/en
Publication of WO2002090992A3 publication Critical patent/WO2002090992A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A screening method is disclosed which enables the identification of biologically active agents which mediate their effects through the activation of genes. The method utilises promoter trap, enhancer trap and gene trap constructs, comprising a reporter molecule, which are transfected into cells which are then cloned into a cell array. The cell array is exposed to an agent(s) to be tested and the response of the cell array to the agent monitored. The method enables both the identification of agents and the genes through which the agents act.
PCT/GB2002/001946 2001-05-04 2002-04-29 Screening method WO2002090992A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002249467A AU2002249467A1 (en) 2001-05-04 2002-04-29 Screening method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0111004.8 2001-05-04
GBGB0111004.8A GB0111004D0 (en) 2001-05-04 2001-05-04 Screening method

Publications (2)

Publication Number Publication Date
WO2002090992A2 WO2002090992A2 (en) 2002-11-14
WO2002090992A3 true WO2002090992A3 (en) 2003-12-04

Family

ID=9914069

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/001946 WO2002090992A2 (en) 2001-05-04 2002-04-29 Screening method

Country Status (3)

Country Link
AU (1) AU2002249467A1 (en)
GB (1) GB0111004D0 (en)
WO (1) WO2002090992A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883891B2 (en) 2001-07-17 2011-02-08 Massachusetts Institute Of Technology Unique properties of stem cells
US7193069B2 (en) 2002-03-22 2007-03-20 Research Association For Biotechnology Full-length cDNA
DE10319478A1 (en) * 2003-04-30 2004-11-18 Universität Zu Köln siRNA selection process
US7618936B2 (en) 2004-05-21 2009-11-17 The Regents Of The University Of California Methods for treating and diagnosing cancer with WNT inhibitory Factor-1 (WIF-1)
US9045553B2 (en) 2004-05-27 2015-06-02 Acceleron Pharma, Inc. Cerberus/Coco derivatives and uses thereof
US7316998B2 (en) 2004-05-27 2008-01-08 Acceleron Pharma Inc. Cerberus/Coco derivatives and uses thereof
US7655465B2 (en) 2004-06-07 2010-02-02 Massachusetts Institute Of Technology Methods for ex vivo propagation of somatic hair follicle stem cells
AU2006261850B2 (en) * 2005-06-22 2011-06-16 Cxr Biosciences Limited Reporter hepatocytes and other cells for drug screening and toxicity testing
US7824912B2 (en) 2005-06-23 2010-11-02 Massachusetts Institute Of Technology Methods for ex vivo propagation of adult hepatic stem cells
WO2008073351A2 (en) 2006-12-08 2008-06-19 Acceleron Pharma Inc. Uses of cerberus, coco and derivatives thereof
WO2015017767A1 (en) * 2013-08-02 2015-02-05 University Of Georgia Research Foundation, Inc. Cryptosporidium transfection methods and transfected cryptosporidium cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO2001007891A2 (en) * 1999-07-27 2001-02-01 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
WO2001019855A2 (en) * 1999-09-13 2001-03-22 American Home Products Corporation Pharmaceutical compositions and methods of using secreted frizzled related protein

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO2001007891A2 (en) * 1999-07-27 2001-02-01 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
WO2001019855A2 (en) * 1999-09-13 2001-03-22 American Home Products Corporation Pharmaceutical compositions and methods of using secreted frizzled related protein

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BAFICO ANNA ET AL: "Interaction of frizzled related protein (FRP) with Wnt ligands and the frizzled receptor suggests alternative mechanisms for FRP inhibition of Wnt signaling.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 23, 4 June 1999 (1999-06-04), pages 16180 - 16187, XP002243190, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
GB0111004D0 (en) 2001-06-27
AU2002249467A1 (en) 2002-11-18
WO2002090992A2 (en) 2002-11-14

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