WO2000049387A3 - High numerical aperture flow cytometer and method of using same - Google Patents

High numerical aperture flow cytometer and method of using same Download PDF

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Publication number
WO2000049387A3
WO2000049387A3 PCT/US2000/004069 US0004069W WO0049387A3 WO 2000049387 A3 WO2000049387 A3 WO 2000049387A3 US 0004069 W US0004069 W US 0004069W WO 0049387 A3 WO0049387 A3 WO 0049387A3
Authority
WO
WIPO (PCT)
Prior art keywords
laser input
flow cell
angle
oriented
right angle
Prior art date
Application number
PCT/US2000/004069
Other languages
French (fr)
Other versions
WO2000049387A9 (en
WO2000049387A2 (en
Inventor
Anthony A Ferrante
W Peter Hansen
Original Assignee
Idexx Lab Inc
Anthony A Ferrante
W Peter Hansen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idexx Lab Inc, Anthony A Ferrante, W Peter Hansen filed Critical Idexx Lab Inc
Priority to EP00914609A priority Critical patent/EP1095256A2/en
Priority to JP2000600079A priority patent/JP2002537557A/en
Priority to CA002329031A priority patent/CA2329031C/en
Priority to AU35976/00A priority patent/AU768616C/en
Publication of WO2000049387A2 publication Critical patent/WO2000049387A2/en
Publication of WO2000049387A3 publication Critical patent/WO2000049387A3/en
Publication of WO2000049387A9 publication Critical patent/WO2000049387A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1447—Spatial selection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The high numerical aperture flow cytometer of the present invention includes a flow cell and a laser input. The laser input emits a beam of light that is oriented substantially orthoganilly to the flow of blood cells through the flow cell such that laser light impinges upon the blood cells as they pass through the flow cell. A portion of the beam from the laser input that impinges upon the blood cells in the flow cell is scattered at a substantially right angle to the beam of laser input ('right angle scatter'). A second portion of the beam from the laser input that impinges upon the cells in the flow cell is scattered at a much lower angle than 90°. This scatter is termed 'low angle forward scatter light' and has an angle of from about 2° to about 5° from the orientation of the original beam from laser input. A right angle scatter light detector is oriented to receive the previously mentioned right angle scatter light. A low angle forward scatter light detector is oriented to capture the previously mentioned low angled forward scatter light oriented at about 2° to about 5° beam from laser input.
PCT/US2000/004069 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same WO2000049387A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00914609A EP1095256A2 (en) 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same
JP2000600079A JP2002537557A (en) 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same
CA002329031A CA2329031C (en) 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same
AU35976/00A AU768616C (en) 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12080499P 1999-02-19 1999-02-19
US60/120,804 1999-02-19

Publications (3)

Publication Number Publication Date
WO2000049387A2 WO2000049387A2 (en) 2000-08-24
WO2000049387A3 true WO2000049387A3 (en) 2001-02-15
WO2000049387A9 WO2000049387A9 (en) 2001-09-27

Family

ID=22392650

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/004069 WO2000049387A2 (en) 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same

Country Status (5)

Country Link
EP (1) EP1095256A2 (en)
JP (1) JP2002537557A (en)
AU (1) AU768616C (en)
CA (1) CA2329031C (en)
WO (1) WO2000049387A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6618143B2 (en) 2000-02-18 2003-09-09 Idexx Laboratories, Inc. High numerical aperture flow cytometer and method of using same
WO2002043486A1 (en) 2000-11-29 2002-06-06 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305832T3 (en) 2003-03-28 2022-05-23 Inguran Llc Method for providing sexed animal semen

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4606636A (en) * 1983-10-25 1986-08-19 Universite De Saint-Etienne Optical apparatus for identifying the individual multiparametric properties of particles or bodies in a continuous flow
US4818103A (en) * 1981-05-15 1989-04-04 Ratcom Flow cytometry
US4954715A (en) * 1989-06-26 1990-09-04 Zoeld Tibor Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer
US5631165A (en) * 1994-08-01 1997-05-20 Abbott Laboratories Method for performing automated hematology and cytometry analysis
US5650847A (en) * 1995-06-14 1997-07-22 Erkki Soini Method and device for determination of parameters of individual microparticles

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8601000A (en) * 1986-04-21 1987-11-16 Jan Greve T H Twente Afdeling THE USE OF POLARIZED LIGHT IN FLOW CYTOMETRY.
US5057413A (en) * 1988-06-13 1991-10-15 Becton, Dickinson And Company Method for discriminating between intact and damaged cells in a sample
AU3271895A (en) * 1994-08-01 1996-03-04 Abbott Laboratories Method and apparatus for performing automated analysis
JP3324050B2 (en) * 1994-10-31 2002-09-17 日本光電工業株式会社 Leukocyte classification reagent and leukocyte classification method
JP3504029B2 (en) * 1995-07-04 2004-03-08 シスメックス株式会社 Particle analyzer
EP0805441B1 (en) * 1996-05-03 2003-10-08 Ciba SC Holding AG High capacity optical recording medium containing xanthene dyes
DE19700648A1 (en) * 1997-01-10 1998-07-23 Basf Ag Method and device for determining the size distribution of different types of particles in a sample
JP3642658B2 (en) * 1997-06-30 2005-04-27 シスメックス株式会社 Urine component analyzer and analysis method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818103A (en) * 1981-05-15 1989-04-04 Ratcom Flow cytometry
US4606636A (en) * 1983-10-25 1986-08-19 Universite De Saint-Etienne Optical apparatus for identifying the individual multiparametric properties of particles or bodies in a continuous flow
US4954715A (en) * 1989-06-26 1990-09-04 Zoeld Tibor Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer
US5631165A (en) * 1994-08-01 1997-05-20 Abbott Laboratories Method for performing automated hematology and cytometry analysis
US5650847A (en) * 1995-06-14 1997-07-22 Erkki Soini Method and device for determination of parameters of individual microparticles

Also Published As

Publication number Publication date
EP1095256A2 (en) 2001-05-02
AU768616B2 (en) 2003-12-18
JP2002537557A (en) 2002-11-05
CA2329031A1 (en) 2000-08-24
WO2000049387A9 (en) 2001-09-27
CA2329031C (en) 2003-09-23
AU768616C (en) 2004-12-16
AU3597600A (en) 2000-09-04
WO2000049387A2 (en) 2000-08-24

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