TW201233341A - Nutritional products comprising calcium beta-hydroxy-beta methylbutyrate and conjugated linoleic acid - Google Patents

Abstract

Disclosed are nutritional products comprising calcium beta-hydroxy-beta methylbutyrate, conjugated linoleic acid, and protein. The nutritional product forms include nutritional liquids and nutritional powders. The nutritional products not only provide benefits for individuals concerned with muscle health and functionality, but also exhibit physical stability, sensory and/or aesthetic benefits in each of the selected product forms.

Description

201233341 VI. Description of the Invention: TECHNICAL FIELD OF THE INVENTION The present invention relates to solid and liquid nutritional products comprising protein, beta-hydroxy-beta methylbutyrate (HMB about) and conjugated linoleic acid (CLA). [Prior Art] Protein-containing nutrient solutions and powders containing nutritionally selected ingredients are well known and widely used in the industry, some of which provide a dedicated source of nutrition, while others provide a complementary source. Such protein-containing nutrients include powders that can be reconstituted with water or other aqueous liquids, as well as ready-to-drink nutrient solutions, such as milk or protein based emulsions or non-emulsified liquids. Protein-containing supplements are typically used to improve or maintain the muscle health of an athlete and an individual at risk of or suffering from a disease or condition associated with skeletal muscle atrophy. Other nutrients known to affect muscle health include beta-hydroxy-p methylbutyrate (ΗΜΒ). Lanthanide is a naturally occurring amino acid metabolite present in a variety of nutritional products and supplements. ΗΜΒ Help build or maintain healthy muscle mass and strength in selected individuals. When calcium is formulated into oral nutritional products, it is commonly used in the form of tablets, capsules, reconstitutable powders, nutrient solutions and lotions. A further nutrient known to be used for bokeh> muscle health is a total of linoleic acid (CLA). CLA generally refers to any one or more of the group of at least 28 isomers of linoleic acid. CLA has been shown to reduce body fat and increase muscle mass' and has also been shown to have anti-cancer and antioxidant properties. Since CLA has a marked bitter taste and causes a burning sensation in the mouth when taken orally, it is most commonly incorporated into capsules and pills to minimize these effects. 161134.doc 201233341 Therefore, it would be desirable to administer proteins, CLA, and HMB to individuals to affect muscle health, particularly because of the different mechanisms or pathways by which the three nutrients provide such effects. It would even be desirable to administer all three ingredients from a single nutritional product (especially a nutrient solution), although this has been a challenge to date due to the need for research and development in each of them. In nutrient solutions, cLA has a bitter taste and causes a burning sensation in the throat, while soluble calcium loading from most HMB sources (or other sources of calcium) can cause many types of protein sinks to be added, making the product potentially unstable. Thus, the present invention relates to nutrient solutions and powders comprising relatively high concentrations of HMB calcium, CLA and protein. The resulting nutritional product is physically stable over shelf life and provides advantageous aesthetic and sensory qualities. SUMMARY OF THE INVENTION One embodiment relates to a conjugated linoleic acid containing from about 1% by weight to about 1% by weight of HMB, about 5% by weight to about 1% by weight, about 1 〇 by weight. % to about 30% by weight of protein and from about 50% to about 98% by weight of nutrient solution of water. Another embodiment relates to a conjugated linoleic acid comprising from about 1% by weight to about 1% by weight of HMB calcium, from about 〇_〇1% by weight to about 1% by weight, and about i 〇% by weight to A nutritional powder of about 40% by weight protein. Another embodiment relates to a nutritional product comprising calcium HMB, conjugated linoleic acid, protein, fat, and carbohydrates. Another embodiment relates to a method of improving the physical stability of a nutritional product comprising HMB calcium. The method comprises introducing a conjugated linoleic acid to the nutritional product, wherein the weight ratio of conjugated linoleic acid to HMB calcium is from about 1.8:1 to about 2.3:1. 161134.doc 201233341 It has been found that the addition of cla to nutritional products (such as nutrient solutions and solids) results in a bitter or white scent of the product and a pronounced throat burning effect after consumption, even at very low levels. It has also been found that many nutrient solutions (e.g., nutritional emulsions) that combine human HMB calcium with protein are not statistically stable, often resulting in the accumulation of excess protein or other deposits at the bottom of the emulsion container, thereby potentially reducing the availability of nutrients and Shorten the effective shelf life of the product. It has now surprisingly been found that these instability and odor problems can be minimized or eliminated by formulating nutritional products with a combination of HMB calcium, CLa and protein. The nutritional products described herein comprise a specific combination of HMB calcium, CLA and protein that not only provides the individual with respect to muscle strength, health and function benefits' but also provides improved physical stability in each of the selected product forms, Sensory and / or aesthetic benefits. [Embodiment] The nutritional product described in this month includes feed and protein. : Equal nutritional products have commercially acceptable taste characteristics and are stable for long periods of time even in liquid form. Some of the characteristics and other features of nutritional products, as well as many of the alternative variations and additions, are described in detail below. π Unless otherwise stated, the term "HMB calcium" as used herein refers to b, β methyl butyrate (also known as hydroxy p-butyric acid, hydroxy _3_: butyl S owing 'β• hydroxy) Calcium salt of valeric acid or hydrazine, which is most commonly present in the form of a single formula. Unless otherwise stated, otherwise used herein to describe the characteristics of the aunt's characteristics, the percentages and concentrations are based on the weight of the calcium monohydrate. . 161134.doc 201233341 The terms "fat" and "oil" as used herein, unless otherwise indicated, are used interchangeably and refer to a lipid material derived from plants or animals or from plants or animals. These terms also include synthetic lipid materials as long as the synthetic materials are suitable for oral administration to humans. The term "storage shelf stability" as used herein means that the nutrient solution is packaged and stored under (4) for at least 3 months (including from about 6 months to about 24, and also includes from about 12 months to about 18). After the month), it remained commercially stable. "Nutritional product" means and is suitable for human mouth. Unless otherwise stated, the term "containing liquids and powders containing protein and one or more fats and carbohydrates for consumption", unless otherwise stated, is used herein. "Nutrition solution" means a nutrient product of the formula (IV) and a nutrient solution prepared by reconstituting the Luyang powder described herein before use. Ratios, parts and ratios are otherwise indicated by the contents, and all percentages used herein are by weight of the total composition, unless otherwise stated. Unless otherwise stated, all such weights as used in the context of the present invention are based on the fact that the active materials may not include solvents or by-products that may be included in the commercially available materials unless otherwise stated or referred to. The context of the plural feature or limitation of the content expressly implies that the content of the singular feature or limitation of the opposite should be construed as the 'or vice versa.' And the context of the combination is expressly implied by the contrary. All combinations of methods or processing steps may be carried out in a sequence unless otherwise stated or modified. 161134.doc 201233341 Various embodiments of the nutritional product of the present invention may also contain no optional or selected essential ingredients or features described herein, provided that the remaining nutritional product still contains as described herein. All the required ingredients or characteristics. Here: In the following, and unless otherwise stated, the term "substantially no choice of nutritional products contains less than the functional amount of the optional ingredients mentioned, 丄^ = the weight of the selected or selected basic ingredients, usually Less than about 1 J A.0/o, including less than about 0.5%, including less than about 〇. 〗 〖%, and also includes 〇%. § Hai and other nutritional products may contain the basic elements of the product as described herein and Any of the additional or optional elements described herein or which may be used in the application of a nutritional product, consist of or consist essentially of the same. Product form The nutritional product of the present invention comprises both liquid and powder forms. The liquid may include solution, suspension Liquids and emulsions. The powders may include any flowable or islandable particulate solids or mirrors which may be diluted with water or other aqueous liquid to form a nutrient solution prior to use. The nutritional products may be formulated with sufficient amounts and amounts of nutrients, To provide a dedicated, primary or supplemental source of nutrition, or to provide specialized nutritional products for individuals with specific diseases or conditions or with targeted nutritional benefits. The compressed or solid nutritional powder, including in the form of a tablet, can be reconstituted by the intended user with a suitable aqueous liquid (usually water or other aqueous liquid) to form a nutrient solution for immediate oral or enteral use. In the meantime, "immediate" use generally means within about 48 hours of reconstitution, more typically within about 24 hours, most commonly immediately after reconstitution, or 161134.doc 201233341 within 20 minutes. The nutritional powders may include spray dried powders, dry mixed powders, and poly

Nutrient solution

The nutrient solution can have a caloric density adjusted to suit the nutritional needs of the end user, but in most cases the liquid comprises from about 1 Torr to about 5 〇〇 kcal / 240 m b including from about 15 〇 to about 35 〇 kcai /24〇(6), and also includes about 200 to about 320 kcal/240 ml. The nutrient solution can have a pH in the range of from about 3.5 to about 8, but most advantageously in the range of from about 4.5 to about 7.5, including from about 55 to about 73, including from about 62 to about 7.2. Although the serving size of the nutrient solution can vary depending on a number of variables, it is typically in the range of from about 1 〇〇ml to about 3 〇〇mi, including from about 15 〇ml to about 250 ml' and also includes about 19 〇m.丨 to about 240 m P-hydroxy-p-methylbutyric acid (HMB). The nutritional product described herein comprises HMB calcium, which means that the products are formulated by the addition of HMB calcium (most commonly monohydrate), or Prepared in other ways with 161134.doc 201233341 containing calcium and HMB in the final product. Any source of HMB is suitable for use in the nutritional products described herein, provided that the final product contains calcium and HMB, but the source is preferably HMB calcium and is most often added to the nutritional product as it is during formulation. As used herein, the term "added M2HMB calcium" means that the calcium salt of HMB (most commonly as the monohydrate calcium salt of HMB) is added to the nutritional product as a source of HMB. While HMB calcium monohydrate is used in the preferred HMB source herein, other suitable sources may include bioavailable forms of HMB as free acid, salt 'anhydrous salt, ester, internal vinegar, or otherwise providing a nutritional product. Other product forms of HMB. Non-limiting examples of suitable HMB salts for use herein include sodium, potassium, magnesium, chromium, calcium hydrated or anhydrous HMB salts, or other non-toxic salt forms. HMB calcium monohydrate is preferred and available from Technical Sourcing International (TSI) in Salt Lake City, Utah, USA. HMB Calcium in Nutrient Solution, by Weight of Nutrient Solution The concentration range can be up to about 10%, including from about 0.01% to about 1%, and also including from about 5% to about 5.0%, and also including from about 0.5% to about 2.0%, and also including from about 〇4% to about 1.5%. The concentration of HMB calcium in the nutritional powder may range from up to about 10% by weight of the nutritional powder, including from about 1% to about 10%, and also from about 1% to about 7.0%, and also includes From about 1.0% to about 5_0%, and also from about 1% to about 4.0%. The %•nutrition product can provide from about 0.5 grams to about 2.5 161134.doc 201233341 grams per serving, including from about 1_0 grams to about 1.7 grams, including about 15 grams of HMB, wherein the serving may range from about 100 ml to about 400. Ml, including from about 15 〇mi to about 34 〇ml, and also including from about 230 ml to about 300 ml of nutrient solution. The serving size can be once a day, twice a day, three times a day or more frequently to obtain the desired amount of nutrients. Conjugated linoleic acid (CLA)

Such nutritional products comprise CLA, which means that the products are formulated by the addition of CLA or otherwise prepared to contain cla in the final product. Any CLA source is suitable for use herein, provided that the final product contains CLA, but the source is preferably CLA and is most commonly added to the nutritional product as it is during the formulation. A variety of CLA sources are commercially available for blending into nutritional products, most of which are in liquid oil form at room temperature and contain up to 1%, more typically 70-95%, of CLA in oil. The concentration of the CLA in the nutrient solution may be in the range of up to about 5%, including about (4) 1% to about 1%, and also including about 5% to about 5.0%, and also including about 1%. Up to about 3%, and also including from about 1 to about 2.0%. The concentration of CLA in the nutritional powder may range from up to about 10%, including from about 0.01% to about 10%, and also includes, by weight of the nutritional powder. It is from about 5% to about 10.0%, and also includes from about 3.0% to about 1%, and also includes from about 3 to about 7.0%. Such nutritional products most typically provide a CLA of from y York to about 5 grams per gram of preparation of the powder, including from about 2 () grams to about 4 (grams), including about gram 161 J34.doc 201233341. In some embodiments, the concentration of CLA and HMB calcium in the nutritional product can be defined by the weight ratio of CLA to HMB calcium. Usually, the reset ratio of (:^8 and 11]^]5 calcium is about 1.2:1.0 to about 3 〇:1 〇, including about! 5:1 to about 2 5:1, including about 1.8:1 to about 2.3:1, and further comprising from about 18:1 to about 21:1. Although the nutritional product has a weight ratio greater than 2: 丨: 1 (: 1^8 and HMB calcium is within the scope of the invention 'but in many In embodiments, it may be desirable to have a weight ratio of CLA to HMB calcium of 2.1:1 f to ensure that there is sufficient sputum from the HMB in the product to sequester and neutralize the CLA and provide as discussed herein. Improved physical stability. In embodiments in which the weight ratio of CLA to HMB calcium is greater than 2.1··1, it may be desirable in some embodiments to add additional sources of calcium to the nutritional product to ensure the presence of sufficient foot in the product. The amount of calcium, whereby the CLA is substantially chelated and neutralized to provide the desired physical stability of the product. Additional sources may include, for example, oxyhydrogen (tetra), carbon _ and/or calcium sulphate. Hydrogen such as hydrogen may also be used. Other neutralizing agents such as magnesium oxide. Nutrients Nutrients These nutritional products further contain proteins and can be packaged in addition to the protocol and CLA external conditions described herein. - or a variety of other macronutrients. Optional macronutrients include lipids and carbohydrates. The large nutrients commonly used herein include any of the proteins known to be used in oral nutritional products in oral nutritional products #, lipids or carbohydrates or The “source” precondition is an optional large amount of nutrients for oral administration:: is safe and effective, and additionally with other ingredients in the nutritional product. 161134.doc 201233341 Optional lipids, carbohydrates and proteins in nutritional products The concentration or amount may vary considerably depending on the particular nutritional application of the product. These optional large nutrients are most commonly formulated within any of the ranges described in the table below. Nutrients (wt% of composition) )

Each value is preceded by the term nutrient (% of total calories). Example A Example B Water-breaking compound 0-100 H 10-70 Lipid 20-65 Protein 0-100 5-40 Example c_ 40-5 (Γ 35-55 15 -25' Each value is preceded by the term protein nutritional product further comprising protein. Suitable for oral nutritional products and compatible with the essential elements and characteristics of such products - protein or its source ^ suitable for: nutrient solution The concentration of the protein in the nutrient solution can range from about to about 15%, and also from about 1% to about 10%, and also from about i% to about 7. For powder implementation. For example, the nutrient powder can be used in the range of about 1.0% to about 50%, and the range of about 10% to about 50%, and includes From about 10% to about 30%. Suitable protein for nutritional products, Goba to t, wood/original non-restrictive gamma, hydrolyzed, partially hydrolyzed or uncontained protein or protein 161134.doc -12· 201233341 Source 'It can be derived from any known cultivator, cautious and appropriate source, such as milk ( For example, protein, whey), animals (eg, meat, fish), Yi (eg, rice, corn), vegetables (eg 'soy or illusion or combinations thereof. Non-limiting examples of such proteins include milk proteins Isolate, milk protein concentrate, road protein isolate, whey protein, casein sodium or casein calcium, whole fat cow's milk, partially or complete skim milk, soy protein isolate, soy protein concentrate, etc. In one embodiment, the protein system comprises at least about 5% sodium caseinate, desirably at least about 60% casein (four), desirably at least about 7% sodium caseinate, desirably at least about 80% sodium caseinate, Desirably at least about 90% sodium caseinate or desirably at least about 1% sodium caseinate. In another specific embodiment, the protein system comprises a combination of protein sources, including casein calcium (or casein sodium). And a soy protein isolate. In another specific embodiment, the protein system comprises a combination of protein sources, including brewed protein sodium (or casein calcium), milk protein concentrate, soy protein isolate, and whey egg The carbohydrate nutritional product may further comprise any carbohydrate suitable for oral nutritional products and compatible with the essential elements and characteristics of the products. The concentration of carbohydrates in the nutrient solution may be based on the weight of the nutrient solution (eg ) ranging from about 5.0% to about 40%, including from about 7.0% to about 30%, including from about 10% to about 25%. In a particular embodiment, the carbohydrate is about 10.2% by weight of the nutrient solution. The amount is present in the nutrient solution. The concentration of the carbohydrate in the nutrient solids may range from about 10% to about 90%, including from about 20% to about 80%, and further into 161134.doc-13. - 201233341 Steps include approximately 40% to approximately 6G%. In a specific embodiment, the carbohydrate is present in the nutritional solids in an amount of about 58% by weight of the nutritional solids. Non-limiting examples of suitable carbohydrates or sources thereof for use in the nutritional products described herein can include maltodextrin, hydrolyzed or modified starch or corn starch, glucose polymer, corn agglutination, corn smashed solids' From rice carbohydrates, sucrose, glucose, fructose, lactose, high fructose corn syrup bees® (eg, maltitol, erythritol, sorbitol), artificial sweeteners (eg U, B) Stuffed with a bell... followed by potassium), stevia, and combinations thereof. Particularly desirable carbohydrates are low dextrose equivalent (DE) maltodextrin. In a specific embodiment, the carbohydrate system comprises a combination of carbohydrate sources, including maltodextrin (as appropriate, low DE maltodextrin) and stringent fat, most commonly in the form of emulsified fat. The basic elements and characteristics of the product are compatible. The nutritional product may further comprise fat, which is suitable for oral nutritional products and is suitable for any of these fats. The fat may be from about 1% to about 5%, including from about 1.0% to about 20%, and also from about 9% to about 15%, and also from about 5% to about 5.0% by weight of the nutrient solution. The amount is present in the nutrient solution. In one (four) embodiment, the nutrient solution comprises a fat in an amount of about 16% by weight of the nutrient solution. The fat may be from about 1% to about 3% by weight, including from about U% to about 20%, and also from about 5% to about 15%, and also from about 5.0% to about 1% by weight, based on the weight of the nutritional solids. Present in nutritive solids. In a specific embodiment 161134.doc 201233341 'nutritional solids include fat in an amount of about 7.5% by weight of the nutritional solids. Suitable fat sources for use herein include any fat or fat source suitable for use in oral nutritional products and compatible with the essential elements and characteristics of such products. Non-limiting examples of suitable fats or sources thereof for use in the nutritional emulsions described herein include coconut oil, fractionated coconut oil, soybean oil, corn oil, alfalfa oil, safflower oil, high oleic safflower oil, MCT oil (medium long chain triglyceride), sunflower oil, high oleic sunflower oil, palm oil and palm kernel oil, palm olein, canola oil (can〇la 〇 (1), seafood oil, cottonseed oil and Combination 0 In a specific embodiment, the fat system comprises a combination of fat sources, including high oleic safflower oil, canola oil, and soybean oil. Manufacturing Method The nutrient solution can be used to make a nutrient solution (including an emulsion, for example based on Any known or suitable method of making a nutritional emulsion of milk. In a suitable manufacturing method, the nutrient solution uses at least three separate slurries to prepare a liquid (including protein (10)), a carbohydrate, a mineral. (CHO-MIN) sputum and protein (piw) poly-liquid in water. The pIF liquid system consists of heating and mixing selected oils (such as fennel oil, corn oil, etc.) and then adding emulsifiers (such as imprinted phospholipids), fat-soluble steep vitamins and total protein in continuous heating and stirring ( For example, milk protein concentrates, etc. are formed. CHO-MIN liquid system is formed by adding the following substances to water under heating and stirring: minerals (such as citric acid _, phosphoric acid gas unloading, sodium sulphate, etc.), trace and ultra-trace minerals (TM/ UTM premix), thickener or suspending agent (Avicel microcrystalline cellulose (Avicel), gelatin (geiian), 161134.doc 201233341 carrageenan), and HMB calcium and CLA. The obtained CHO-MIN slurry is kept under continuous heating and stirring for 1 minute, and then other minerals (such as potassium chloride, magnesium carbonate, potassium iodide, etc.), carbohydrates (such as fructooligosaccharide, sucrose, corn poly, etc.) are added. ). Then, the pIW slurry is formed by mixing the remaining protein (e.g., casein sodium, soy protein concentrate, etc.) into water under heating and stirring. The resulting slurry is then blended together under heat and agitation, and the pH is adjusted to the 'monthly range (usually 6.6-7. 0), and then the composition is treated with high temperature for a short time (HTS), during which the composition is heat treated. , emulsification and homogenization, then cold. The water-bath vitamins and ascorbic acid are added, the pH is adjusted to the desired range (if needed), the perfume is added, and water is added to achieve the desired total solids content. The composition is then aseptically packaged, formed into a sterile packaged nutritional emulsion, or "and the mouthpiece is added to a sterilizer stable container and then sterilized by a sterilizer to form a sterilized sterilized nutritional emulsion. The invention may be practiced otherwise than as described herein without departing from the spirit and scope of the invention. The present invention is to be considered as illustrative and not limiting, and all changes and equivalents are also in the invention. The nutrient solids (e.g., spray-dried nutritional powders) can be prepared by any combination of known or other effective techniques suitable for making and formulating spray-dried nutritional powders. Aj 0 > J-like includes any of the dry-coal dry coal technologies known to be used to produce nutritional powders or for the production of nutritional powders. Many different spray drying methods and techniques are known for use in A-covers, in the field of nutrition. , all such methods and techniques are ϊ 6ϊ 134.doc -16- 201233341 Suitable for the manufacture of spray-dried nutritional powders herein. The method for preparing a gas-sprayed dry nutritional powder comprises forming An aqueous slurry or liquid comprising hydrazine, octa and protein, and optionally carbohydrates and fats, and homogenized' and then spray-dried the slurry or liquid to produce a spray-dried nutritional powder. The method of financing may include (iv) drying, Dry mixing or the step of adding additional nutrients (including any one or more of the ingredients described herein) to the mouth-dried nutrient powder. The method of manufacturing is preferably strontium calcium (which is most commonly used as HMB calcium). The monohydrate feeds are blended as a source of HMB for use in such methods. The optional ingredients, the nutritional products described herein may further comprise other optional ingredients which may be changed when used in a private group. The physical, nutritional, chemical, pleasure or handling characteristics of the product or the medicinal or additional nutritional components of the product. Many of these optional wounds are known or suitable for use in other nutritional products and can also be used for the maintenance of the products in this article. In βσ, it is stated that these optional ingredients are safe and effective for oral administration and are compatible with the essential and other ingredients in the selected product form. Restrictive examples include preservatives, antioxidants, emulsifiers, buffers, oligofructose, bismuth, acid (Mountain (10) P · Unate) ^ pharmaceutically active substances, additional nutrients as described herein, colorants, flavors, Thickeners, stabilizers, etc. These products may further contain vitamins or related nutrients, non-limiting examples of which include vitamin A, vitamin D, vitamin E, vitamin κ, sulfur 161134.doc • 17- 201233341 Amine, nuclear yellow素..."alcohol, vitamin B12, carotenoid (four), acid test, folic acid, pantothenic acid, biotin, vitamin C, biliary test, inositol, its salts and derivatives, and combinations thereof. The step comprises minerals, non-limiting examples of which include broken towns, iron, zinc, lanthanum, copper, _, _, #, ^1 compounds, and combinations thereof. These products may also include - or a plurality of bridge or masking agents. Suitable bridging agents or masking agents include natural and artificial sweeteners, sodium sources (such as sodium carbonate), and hydrophilic colloids (such as guar gum, xanthan gum, carrageenan, knots). Lacquer ((iv) 丨 (10) gum), gum arabic (four) claw acacia) and combinations thereof. HOW TO USE These nutritional products can be used as a source of nutrition in individuals and sports M and help promote the formation and maintenance of healthy muscles and to enhance performance endurance. In addition, these nutritional products are particularly suitable for elderly and elderly individuals who need to maintain healthy muscle mass, strength and function. These nutritional products can help alleviate the weakness of older adults and improve muscle tone. The methods are intended to be used after each day of administration, most often after a period of from about 1 month to about 6 months (including about 1 month to about 3 months). Individuals provide one or more of the following: 7 4 , 1 ) Maintaining and growing lean body, 2) Supporting strength and/or maintenance of muscle strength and '3' reducing protein breakdown and muscle cell damage' 4) promoting exercise or muscle recovery after trauma, and 5) reducing muscle protein after exercise. Example 161134.doc 18 201233341 The following examples illustrate specific embodiments of the nutritional products of the present invention and or It is intended that the scope of the invention be construed as being limited by the scope of the invention. All exemplified amounts are by weight based on the total weight of the composition, unless otherwise stated. The exemplified composition is a nutritional product prepared by a manufacturing method well known in the nutrition industry for preparing a nutrient solution and a spray-dried nutritional powder. Examples 1-5 Real 5 explains the nutritional powder of the present invention comprising 1 word, CLA and protein. The ingredients thereof are listed in the following table. The product can be prepared in a single (four) times by spray drying, and the target concentration can be reconstituted before preparation. Unless otherwise stated, all ingredients are listed in a batch of kgmoo kg. ~~--- Maltodextrin---- Example 1 Example 2 A"iii η Example 3 Example 4 Example 5~] Sucrose __~~~----- Casein A---- • 0 0 · / 145.5 145.5 436.7 145.5 436.7 145.5 436.7 145.5 ~ "CLA ------ 129.1 129.1 129.1 129.1 129.1 The big ugly separation --- 67.0 63.65 70.35 60.3 73.7 FOS powder '----- 57.7 57.7 57.7 61.7 57.7 HO sunflower i ~~~-- 33.6 33.6 33.6 33.6 32.6 HMB Calcium---~~~-_ 33.1 30.1 36.1 33.1 33.1 Fenhua oil" *---- 31.6 34.6 28.6 27.6 32.6 ~ Large Soybean oil ~~" '---- 28.3 28.3 28.3 28.3 28.3 Potassium citrate~~~~'------ 13.3 13.3 Γ 13.3 13.3 13.3 Sodium citrate·~~~--- 10.3 10.3 10.3 10.3 10.3 Vaporized potassium~---- 5.8 5.8 5.8 5.8 5.8 Magnesium chloride~·~---- 5.2 5.2 5.2 5.2 5.2 Potassium argon oxide '~·~----- 4.7 4.7 4.7 4.7 4.7 Filling acid nitrogen one nano-three —~~-— 3.1 3.1 3.1 3.1 3.1 ------— ^ 〇切化化化-_ 3.0 3.0 3.0 3.0 3.0 Gasification biliary test ——— — 2.5 2.5 2.5 2.5 2.5 " —~~~~~ ~_ 1.8 1.8 1.8 1.8 1.8 161134.doc •19· 201233341

Vanilla Spice 1.8 1.8 1.8 1.8 1.8 Sodium Dihydrogen Phosphate Monohydrate 1.6 1.6 1.6 1.6 1.6 Dipotassium Hydrogen Phosphate Trihydrate 1.1 1.1 1.1 1.1 1.1 Perfume 1.0 1.0 1.0 1.0 1.0 Vitamin Premix 1.0 1.0 1.0 1.0 1.0 Ascorbate 0.243 0.243 0.243 0.243 0.243 Ascorbic acid 0.240 0.240 0.240 0.240 0.240 Antioxidant 0.116 0.116 0.116 0.116 0.116 Ferrous sulfate 0.092 0.092 0.092 0.092 0.092 Vitamin premix 0.065 0.065 0.065 0.065 0.065 Zinc sulfate monohydrate 0.057 0.057 0.057 0.057 0.057 Sulfuric acid 0.045 0.045 0.045 0.045 0.045 Mineral mixture copper sulfate 0.035 0.035 0.035 0.035 0.035 B carotene 0.005 0.005 0.005 0.005 0.005 Chromium chloride 0.001 0.001 0.001 0.001 0.001 In the case of sodium citrate 0.0012 0.0012 0.0012 0.0012 0.0012 Potassium moth 0.001 0.001 0.001 0.001 0.001 Sodium selenite 0.0004 0.0004 0.0004 0.0004 0.0004 Citric acid AN AN AN AN AN KOH AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN 6-10 Examples 6-10 illustrate examples of nutritional emulsions of the present invention, the ingredients of which are set forth in the table below. Unless otherwise stated, all quantities are listed in kilograms per 1000 kilograms of product. Ingredient Example 6 Example 7 Example 8 Example 9 Example 10 Appropriate amount of water Appropriate amount of appropriate amount of sucrose 89.3 89.3 89.3 89.3 89.3 Maltodextrin 29.7 29.7 29.7 29.7 29.7 Casein sodium 25.9 25.9 25.9 25.9 25.9 Milk protein concentrate 19.1 19.1 19.1 19.1 19.1 CLA 14.2 13.5 14.9 12.8 15.6 Soy protein isolate 11.9 11.9 9.9 12.9 13.9 Potassium citrate 7.9 7.9 7.9 7.9 7.9 Soybean oil 6.4 5.4 6.4 6.4 6.4 HMB calcium 6.7 7.7 8.7 5.7 4.7 161134.doc -20· 201233341 Camellia oil 5.5 5.5 5.5 5.5 5.5 Corn oil 4.6 4.6 4.6 4.6 4.6 Whey protein concentrate 3.5 3.5 3.5 3.5 3.5 Magnesium hydrogen phosphate 3.1 3.1 3.1 3.1 3.1 Perfume 2.0 2.0 2.0 2.0 2.0 Microcrystalline cellulose 2.0 2.0 2.0 2.0 2.0 Soy lecithin 1.5 1.5 1.5 1.5 1.5 Hydrogen phosphate Disodium dihydrate 1.3 1.3 1.3 1.3 1.3 Dipotassium hydrogen phosphate 0.985 0.985 0.985 0.985 0.985 Potassium chloride 0.729 0.729 0.729 0.729 0.729 Choline chloride 0.480 0.480 0.480 0.480 0.480 Ascorbic acid 0.469 0.469 0.469 0.469 0.469 Calcium carbonate 0.451 0.451 0.451 0.451 0.451 Sugar Spice 0.450 0.450 0.450 0.450 0.450 Creamer 0 .450 0.450 0.450 0.450 0.450 UTM/TM Premix 0.367 0.367 0.367 0.367 0.367 45% Potassium Hydroxide 0.323 0.323 0.323 0.323 0.323 Carrageenan 0.200 0.200 0.200 0.200 0.200 Water Soluble Vitamin Premix 0.185 0.185 0.185 0.185 0.185 Vitamin DEK Premix 0.067 0.067 0.067 0.067 0.067 Sodium chloride 0.060 0.060 0.060 0.060 0.060 Lancture 0.050 0.050 0.050 0.050 0.050 Vitamin A palmitate 0.0082 0.0082 0.0082 0.0082 0.0082 Corn oil carrier q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q q 194 mg 194 mg 194 mg 194 mg 194 mg Example 11 In this example, the stability of the nutritional emulsion including CLA, HMB, and protein was analyzed and compared to a control sample emulsion without CLA. Formulations for both the control sample nutritional emulsion and the nutritional emulsion including CLA are set forth in the table below. Unless otherwise stated, all ingredients are listed in kilograms per 1000 kilograms of product. These emulsions are prepared using the usual methods described herein. Ingredients Control Appropriate amount of sucrose containing CLA 89.3 89.3 161134.doc •21 - 201233341 Maltodextrin 29.7 29.7 Road protein nano 25.9 25.9 Milk protein concentrate 19.1 19.1 CLA 0.0 14.2 Soy protein isolate 11.9 11.9 Potassium citrate 7.9 7.9 Soybean oil 6.4 6.4 HMB calcium 6.7 6.7 Canola oil 5.5 5.5 Corn oil 4.6 4.6 Whey protein concentrate 3.5 3.5 Magnesium hydrogen phosphate 3.1 3.1 Perfume 2.0 2.0 Microcrystalline cellulose 2.0 2.0 Soy lecithin 1.5 1.5 Disodium hydrogen phosphate dihydrate 1.3 1.3 Potassium hydrogenate 0.985 0.985 Potassium chloride 0.729 0.729 Gasification choline 0.480 0.480 Ascorbic acid 0.469 0.469 Calcium carbonate 0.451 0.451 Spice 0.450 0.450 Creamer 0.450 0.450 UTM/TM premix 0.367 0.367 45% hydroxide if 0.323 0.323 Carrageenan 0.200 0.200 Water Soluble Vitamin Premix 0.185 0.185 Vitamin DEK Premix 0.0675 0.067 Gasified Sodium 0.0601 0.060 Starch 0.0500 0.050 Vitamin A Palmitate 0.0082 0.0082 Corn Oil (Carrier) Appropriate amount of vitamin d3 399 mg 399 mg Moth 194.4 mg 194.4 mg will be a nutritional emulsion Mounted in 240-ml plastic container and a method commonly used to carry out sterilization autoclave sterilization. Visual inspection surprisingly showed that after 12 months of storage at ambient temperature, nutritional emulsions including CLA and HMB calcium showed less sediment than controls including HMB calcium but excluding CLA (see Figure 1; The control sample and the right side are emulsions containing CLA). In other words, the nutritional milk 161134.doc -22- 201233341 solution includes CLA surprisingly improving the physical stability of the nutritional product containing HMB calcium after 12 months due to the formation of precipitates at the bottom of the container and the precipitation of significantly less precipitation. Example 12 In this example, a nutritional emulsion comprising CLA, HMB and protein, and a control sample without CLA were analyzed for the taste quality. In particular, since CLA provides a bitter taste and imparts strong throat irritation after direct ingestion, it is difficult to incorporate this functional ingredient into the nutritional product without significantly impairing the taste of the product to a commercially undesirable level. The sample emulsion was analyzed by the §;j sensory panel for the intensity of various flavors: sweet, salty, sour, bitter, flavor intensity, taste intensity (base) Intensity) and hope. Specifically, after the emulsion was prepared, 5 members of the training group consumed 8 nutrient ounces of each nutrient emulsion. After each lotion, each group member used the following table. The analysis tool (pr〇filer) listed in the article gives a rating to the taste. The average of the results is then taken.

As shown in the results table below, it was surprisingly found that the taste quality of the nutritional emulsion including CLA was similar to that of the control sample excluding any CLA 161134.doc • 23· 201233341. Notably and surprisingly, there is no difference in bitterness between the samples. These results indicate that a nutritional emulsion containing CLA has similar taste characteristics to a control emulsion that does not include CLA and would be commercially acceptable from a taste point of view. In addition, the results indicate that a nutritional product having acceptable taste qualities, including both CLA and HMB calcium in combination with protein, can be formulated. Sample Taste Analysis Tool Results Control Sample (HMB only) Sweet 1 '/2 Sour 1 Dirty '/2 Bitterness - Odor Strength - Base Taste Strength 1 '/2 Hope - CLA+HMB Nutritional Emulsion Sweet Flavor 1 '/2 sour taste 1 thief taste '/2 bitterness - odor intensity - alkali taste intensity 1 'Λ 盼味1 [Simple diagram of the diagram] Figure 1 shows two samples for evaluation of stability and precipitation in Example 11. Liquid nutritional formula. 161134.doc -24-

Claims (1)

201233341 VII. Patent application scope: 1. A nutrient solution comprising about 0. 01% by weight to about 1 〇 by weight of base-β methylbutylate, about 0.01% by weight to about 1% by weight of the total vehicle. Oleic acid, about i weight. /. Up to about 30% by weight protein and from about 5% by weight to about 98% by weight water. 2. The nutrient solution of claim 1, which comprises from about 1% by weight to about 5% by weight of calcium β-hydroxy-β methylbutyrate. 3. The nutrient solution of claim 1, which comprises from about 5% by weight to about 2% by weight. Calcium β-hydroxy-β methylbutyrate. 4. The nutrient solution as claimed in the present invention, which comprises from about 5% by weight to about 5% by weight of a total amount of linoleic acid. 5. The nutrient solution of claim 1, which comprises from about 1% by weight to about 3% by weight of total light linoleic acid. 6. The nutrient solution of claim 1, which comprises from about 5% by weight to about 7% by weight of protein. 7. The nutrient solution of claim 1, wherein the liquid system further comprises from about 5% by weight to about 30% by weight of the aqueous emulsion of the fat. 8. The nutrient solution of claim 1, wherein the liquid system is packaged in a hermetic seal, the container and the shelf life is stable for at least about 3 months at a temperature of from about 18 °C to about 24 °C. X. 9. The nutrient solution of claim ,, wherein the weight ratio of conjugated linoleic acid to calcium hydroxy-β-mercaptobutyrate is from about 1.8:1 to about 2.3:1. 1营养· A nutritional powder comprising from about 0.01% by weight to about 1% by weight of hydroxy-β-mercaptobutyric acid, from about 0.01% by weight to about 1% by weight of total light: 161134.doc 201233341 And about 1% to about 5% by weight of the nutritional powder of claim 10, % of β-hydroxy-β-methylbutyric acid, such as the nutritional powder of claim 10, % of 0-hydroxy-β methyl Calcium butyrate. A nutrient powder of claim 10, a total of linoleic acid. 11. 12. 13. 14. 15. % by volume of protein. It comprises from about 0.1% by weight to about 10% by weight of the nutritional powder comprising from about 1.0% by weight to about 5.0% by weight of the compound comprising from about 3% by weight to about 7. 〇 by weight, wherein the conjugated linoleum The weight ratio of acid to calcium hydroxymethyl methacrylate is from about 18:1 to about 2 3:1. A nutritional product comprising β-trans-β-mercaptobutyric acid, conjugated linoleic acid, protein, fat, and carbohydrates. 161134.doc