SU502019A1 - Strain - Google Patents

Description

(54) BREViB.ACTERIU; U 22Л-Д

This invention relates to the Microbiological Application, Schlenology and concerns iHOBoro st.mma Brevibacterium 22L-D used to obtain L-lysine.

The proposed, in contrast to the known ones, has a wide KOKI1M spectrum of anomalous carbon sources, including Haiti, the ability to use instead of TA-1M | Ipa only its pyrime; h acids.

The proposed shta; mm is selected; it is made of shta.mma Brevibacterium 22L and is characterized by the following 1M | AND properties.

Morphology. On form.-cells oval or round, short sticks the size

1.0-2.8

divided by partition, form

a1 dialogues. mesospond mesosomes and polyphosphate grana; 1s, no-frost, g mM positive,, non-PO; Motion.

Organic Wednesday. M so-peptany ogar. The colonies are small, round with a smooth awn noBeipxiH, pygmy-ittirova yellow orange color.

Relation to the source: ni1ka; m carbon. Assimilates glucose, fructose, sucrose, maltose, halo to cattle, mannose, acetic acid, ethyl alcohol, products of starch and cellulose hydrolysis.

Relation to istachinkai nitrogen. Assimilates ammonium chloride, ammonium sulphate, urea, dvumesh.en, ny ammonium phosphate; 1st. In a medium with peptone, a1miak is released.

Uses aspartic acid, tyrosine, isoleation, series, fnilalanine, prolii, threonine, arginine, histidine, methionine.

Medium riboflavin and folic acid are released.

Use the pyramidal part of thiamI: On.

: rahmal. Does not hydrolyze.

Gelatin. Does not dilute.

0 | Blat catalase and ureaznoy zkti / VNUTYU.

Example. Sowing material is prepared: on the medium of the following composition (in%);

Glucose2

Corn extract4

Nad0.3

pH 7,: 2

the medium is sterilized at 1 ppm for 30 minutes. Sow in the crook 24-hour culture of Brevibacterium 22L-D. The inoculum is grown; from a S volume of 60 ml in 0.7 l flasks on a rocking chair for 24 hours, dri 30-32 ° C and at 200 rpm. 4-5% inoculum is introduced into the iro-production medium.

Fermentation is carried out on the next section of the compound (%):

Molasses 6 (lo sugar)

Case 3: New Exp. 2

(NH4) 2S042

.rn 7.2.

The medium is sterilized with

1 ATI 30 min.

Service; B; And fermentation: temperature 30–32 ° C, stirring speed 500 rpm, air 0.8 volume per volume of medium, ipH 6.8-7.2 is maintained by adding 10% - | New pacTiBOr capric acid and; 1I liO % am, miak.

After 1 cyTiKH, a sterile solution of memeases is periodically applied to the total content of p in the medium of 12% (momentum; the 1st value is not higher than 6%) periodically. Time fer; mentation) 50-62 hours. Li3 concentration .HiH: a 36 g / l.

After fermentation, the culture liquid is stabilized with hydrochloric acid and sodium bisulfate M, and dehydrated, for example, in an evaporation unit, followed by drying in a hot-air dryer. The finished product is a carbon dioxide powder with a lisine content of mo; i.o. hydrochloride not less than 17%.

3 pp. The case of obtaining lysium in the | historical form, this is isolated by one of the well-known methods; for example, using ioooob: e: iHHx resins.

Formula and 3 o b e e N

Strains: m Brevibacterium 22L-D - L-lysna production.

four

olo: n Oval or round K1etkms, sticks 1.0-2.8 microns in size, are divided by a septum; they form analogs of mltochandry.

Orga: Nicaea Wednesday. M so-peptolny. Coloippus are small, round with a smooth surface, and are yellow-o, pa; nzhevym zet.

The ratio of | And: STOCHNIKA1G h carbon. AssiMil Irate glucose, fructose, sucrose, mgltozy, galactose, mapnosis, acetic acid, ethyl alcohol, hydrolysis products (rahmal and cellulose.

Relation to nitrogen sources. Assimilate. Am.orium chloride, alum sulphate: MS1n; d, disubstituted ammonium phosphate, urea. Amount of ammonia is leased with peptone.

It uses aoparapine acid, gprozi, isoleudine, series, fevilalan / n, prolip, treac, I1H, arti1N1I1H, histidine, metiscnin.

Medium riboflavin and folioBuY -K | And | SLOT are released on Wednesday.

Uses pi rI | M IDIOLNOU chasti st.

K | rah1mal not gi, drolizuet. Gelatinu cannot be dilated, izhat, has catalase and urease akti1enassty.