SE0500707L - New Xylanase - Google Patents

New Xylanase

Info

Publication number
SE0500707L
SE0500707L SE0500707A SE0500707A SE0500707L SE 0500707 L SE0500707 L SE 0500707L SE 0500707 A SE0500707 A SE 0500707A SE 0500707 A SE0500707 A SE 0500707A SE 0500707 L SE0500707 L SE 0500707L
Authority
SE
Sweden
Prior art keywords
sup
enzyme
activity
xylanase
inhibition
Prior art date
Application number
SE0500707A
Other languages
Swedish (sv)
Inventor
Bo Mattiasson
Rajni Hatti-Kaul
Gashaw Mamo
Original Assignee
Forskarpatent I Syd Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forskarpatent I Syd Ab filed Critical Forskarpatent I Syd Ab
Priority to SE0500707A priority Critical patent/SE0500707L/en
Priority to PCT/SE2006/000391 priority patent/WO2006104448A1/en
Publication of SE0500707L publication Critical patent/SE0500707L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • C12N9/42—
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
    • D21C9/001—Modification of pulp properties
    • D21C9/002—Modification of pulp properties by chemical means; preparation of dewatered pulp, e.g. in sheet or bulk form, containing special additives

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Animal Husbandry (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A thermostable, alkaline active xylanase was purified from an alkaliphilic Bacillus halodurans S7, isolated from a soda lake in the Ethiopian Rift Valley. The molecular weight and the pi of this enzyme were around 43 kDa and 4.5, respectively. The optimum temperature for the activity was 75 °C at pH 9 and 70 °C at pH 10. The enzyme was stable over a broad pH range and showed good thermal stability when incubated at 65 °C in pH 9 buffer. The enzyme activity was strongly inhibited by Mn<SUP>2+</SUP> with partial inhibition in the presence of 5 mM Cu<SUP>2+</SUP>, Co<SUP>2+</SUP> and EDTA. Inhibition by Hg<SUP>2+</SUP> and dithiothreitol was insignificant. The enzyme was free from cellulase activity. The properties of the xylanase produced by this organism are very attractive for use in pulp treatment and recycling, detergent formulation, feed applications, separation of starch and gluten and waste management.
SE0500707A 2005-03-30 2005-03-30 New Xylanase SE0500707L (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE0500707A SE0500707L (en) 2005-03-30 2005-03-30 New Xylanase
PCT/SE2006/000391 WO2006104448A1 (en) 2005-03-30 2006-03-30 Thermostable alkaline xylanase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0500707A SE0500707L (en) 2005-03-30 2005-03-30 New Xylanase

Publications (1)

Publication Number Publication Date
SE0500707L true SE0500707L (en) 2006-10-01

Family

ID=37053642

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0500707A SE0500707L (en) 2005-03-30 2005-03-30 New Xylanase

Country Status (2)

Country Link
SE (1) SE0500707L (en)
WO (1) WO2006104448A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008095057A2 (en) 2007-01-31 2008-08-07 Novozymes A/S Heat-stable carbonic anhydrases and their use
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
US20110108222A1 (en) * 2009-11-11 2011-05-12 International Paper Company Effect of low dose xylanase on pulp in prebleach treatment process
EP2468951A1 (en) 2010-12-21 2012-06-27 Realco S.A. Method for processing cellulosic materials
CN103437231B (en) * 2013-08-30 2016-04-13 华南理工大学 A kind of alkaline enzymatic deinking agent and the technique for applying in deinking thereof
CN104807764A (en) * 2015-04-21 2015-07-29 华南理工大学 Alkaline xylanase activity determination method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2156048C (en) * 1993-12-24 2010-11-16 Pieter Van Solingen Alkali-tolerant xylanases

Also Published As

Publication number Publication date
WO2006104448A1 (en) 2006-10-05

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