NL2032828B1 - Microbial inoculant for composting livestock and poultry manure and preparation method thereof - Google Patents
Microbial inoculant for composting livestock and poultry manure and preparation method thereof Download PDFInfo
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- 239000002054 inoculum Substances 0.000 title claims abstract description 38
- 230000000813 microbial effect Effects 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 238000009264 composting Methods 0.000 title claims abstract description 23
- 210000003608 fece Anatomy 0.000 title claims abstract description 23
- 244000144972 livestock Species 0.000 title claims abstract description 23
- 239000010871 livestock manure Substances 0.000 title claims abstract description 23
- 244000144977 poultry Species 0.000 title claims abstract description 23
- 241000894006 Bacteria Species 0.000 claims abstract description 41
- 241000589149 Azotobacter vinelandii Species 0.000 claims abstract description 21
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000011591 potassium Substances 0.000 claims abstract description 21
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 21
- 241000589180 Rhizobium Species 0.000 claims abstract description 20
- 241000223259 Trichoderma Species 0.000 claims abstract description 20
- 241000187395 Streptomyces microflavus Species 0.000 claims abstract description 17
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 15
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 15
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 14
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 14
- 241001465752 Purpureocillium lilacinum Species 0.000 claims abstract description 14
- 230000001580 bacterial effect Effects 0.000 claims description 40
- 239000001963 growth medium Substances 0.000 claims description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 244000068988 Glycine max Species 0.000 claims description 9
- 235000010469 Glycine max Nutrition 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 238000011049 filling Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 7
- 238000009630 liquid culture Methods 0.000 claims description 7
- 238000004806 packaging method and process Methods 0.000 claims description 7
- FYBLWUVZITWWEZ-UHFFFAOYSA-N Cl.[Ca] Chemical compound Cl.[Ca] FYBLWUVZITWWEZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 238000003860 storage Methods 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000011218 seed culture Methods 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims 2
- 238000012856 packing Methods 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 8
- 244000052616 bacterial pathogen Species 0.000 abstract description 5
- 230000002401 inhibitory effect Effects 0.000 abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000035558 fertility Effects 0.000 abstract description 2
- 239000003337 fertilizer Substances 0.000 abstract description 2
- 230000001965 increasing effect Effects 0.000 abstract description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 230000004936 stimulating effect Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 18
- 150000001875 compounds Chemical class 0.000 description 9
- 238000012258 culturing Methods 0.000 description 9
- 229930006000 Sucrose Natural products 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 8
- 229960001031 glucose Drugs 0.000 description 8
- 235000001727 glucose Nutrition 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- 108010077805 Bacterial Proteins Proteins 0.000 description 6
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 6
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 241000187747 Streptomyces Species 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 239000000787 lecithin Substances 0.000 description 4
- 229940067606 lecithin Drugs 0.000 description 4
- 235000010445 lecithin Nutrition 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/465—Streptomyces
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Abstract
The present invention discloses a microbial inoculant for composting livestock and poultry manure and a preparation method thereof. The present invention relates to the technical field of microbial inoculants, including the following components: bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and rhizobium astragali, by the use of this inoculant, it may not only have the ability of improving the survivability in a soil environment, converting nitrogen and potassium in soil, improving the soil fertility, and stimulating the crop growth, but also reduce the amount of chemical fertilizers, inhibit the reproduction of germs, and achieve the three—in—one effect of increasing the production and income, inhibiting diseases, and improving the quality, so the input cost is reduced.
Description
P1539/NLpd
MICROBIAL INOCULANT FOR COMPOSTING LIVESTOCK AND POULTRY MANURE
AND PREPARATION METHOD THEREOF
The present invention relates to the technical field of mi- crobial inoculants, in particular to a microbial inoculant for composting livestock and poultry manure and a preparation method thereof.
Existing microbial inoculants for composting livestock and poultry manure have relatively tedious production steps and rela- tively complicated operations, which not only increase the input cost, but also produce finished products with relatively weak bac- tericidal and bacteriostatic effects, and the survivability in a soil environment is relatively poor, so it may not help the growth of crops better. For this reason, persons skilled in the art pro- vide a microbial inoculant for composting livestock and poultry manure and a preparation method thereof.
In view of the deficiencies of the prior art, the present in- vention provides a microbial inoculant for composting livestock and poultry manure and a preparation method thereof, as to solve problems that existing microbial inoculants for composting live- stock and poultry manure are relatively high in input cost, fin- ished products produced are relatively weak in bactericidal and bacteriostatic effects, and the survivability in a soil environ- ment is relatively poor, so it may not help the growth of crops better.
In order to achieve the above purpose, the present invention is achieved by the following technical schemes: a microbial inocu- lant for composting livestock and poultry manure, including the following components: bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum,
streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and rhizobium astragali; and the microbial inoculant for composting the livestock and poultry manure is prepared from the following components in parts by mass: 2.0-3.0 parts of the bacillus subtilis, 2.6-3.7 parts of the azotobacter vinelandii, 1.8-2.4 parts of the paecilomyces li- lacinus, 3.3-4.0 parts of the bacillus coagulans, 2.1-3.1 parts of the trichoderma harzianum, 4.0-4.2 parts of the streptomyces mi- croflavus, 0.6-0.8 parts of the trichoderma, 0.3-0.4 parts of the organophosphorus bacteria, 0.3-0.4 parts of the potassium bacteria and 0.5-1.0 part of the rhizobium astragali.
As a further technical scheme of the present invention, it is prepared from the following components in parts by mass: 2.0 parts of the bacillus subtilis, 2.6 parts of the azotobacter vinelandii, 1.8 parts of the paecilomyces lilacinus, 3.3 parts of the bacillus coagulans, 2.1 parts of the trichoderma harzianum, 4.0 parts of the streptomyces microflavus, 0.6 parts of the trichoderma, 0.3 parts of the organophosphorus bacteria, 0.3 parts of the potassium bacteria and 0.5 parts of the rhizobium astragali.
As a further technical scheme of the present invention, it is prepared from the following components in parts by mass: 2.5 parts of the bacillus subtilis, 3.0 parts of the azotobacter vinelandii, 2.1 parts of the paecilomyces lilacinus, 3.6 parts of the bacillus coagulans, 2.6 parts of the trichoderma harzianum, 4.1 parts of the streptomyces microflavus, 0.7 parts of the trichoderma, 0.35 parts of the organophosphorus bacteria, 0.35 parts of the potassi- um bacteria and 0.7 parts of the rhizobium astragali.
As a further technical scheme of the present invention, it is prepared from the following components in parts by mass: 3.0 parts of the bacillus subtilis, 3.7 parts of the azotobacter vinelandii, 2.4 parts of the paecilomyces lilacinus, 4.0 parts of the bacillus coagulans, 3.1 parts of the trichoderma harzianum, 4.2 parts of the streptomyces microflavus, 0.8 parts of the trichoderma, 0.4 parts of the organophosphorus bacteria, 0.4 parts of the potassium bacteria and 1.0 part of the rhizobium astragali.
As a further technical scheme of the present invention, the streptomyces microflavus is mainly fermented from a liquid culture medium of soybean cake powder, glucose, a calcium hydrochloride and a sodium chloride.
As a further technical scheme of the present invention, the content of the soybean cake powder is 3 g, the content of the glu- cose is 3 g, the content of the calcium hydrochloride is 0.5 g, and the content of the sodium chloride is 0.4 g.
As a further technical scheme of the present invention, the azotobacter vinelandii and the potassium bacteria are both cul- tured in an Ashby medium, the trichoderma is cultured in a potato sucrose medium, the organophosphorus bacteria are cultured in a lecithin medium, and the rhizobium astragali is cultured in a yeast juice sucrose medium.
The preparation method for the above microbial inoculant for composting the livestock and poultry manure includes the following steps:
Sl. strain culture: inoculating the bacillus subtilis, azoto- bacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, or- ganophosphorus bacteria, potassium bacteria and rhizobium astraga- li respectively in each culture medium for culturing;
S2. bacterial solution preparation: putting the various bac- terial strains cultured in the Step S1 into a drug bottle of a seed culture medium in a mode of slant surface inoculation, and using a liquid culture medium for culturing separately, to obtain bacterial solution of the bacterial strain;
S3. bacterial solution mixing: putting all types of the bac- terial solution prepared in the Step S2 into a fermentation tank loaded with seeds, stirring and mixing, to obtain composite seed solution;
S4. compound bacterial solution preparation: inoculating all the seed solution in the Step S3 into a seed tank loaded with a fermentation culture medium for fermenting and culturing, to ob- tain compound bacterial solution;
S5. microbial inoculant preparation: drying and powder- spraying the compound bacterial solution in the Step 54, to obtain a finished product microbial inoculant; and
S6. packaging and storing: adding the finished product micro-
bial inoculant in the Step S5 to a filling machine, packaging it into a container by using the filling machine, and putting it in a storage.
BENEFICIAL EFFECT
The present invention provides a microbial inoculant for com- posting livestock and poultry manure and a preparation method thereof. Compared with the prior art, it has the following benefi- cial effects: 1. A microbial inoculant for composting livestock and poultry manure and a preparation method thereof, by using this inoculant, it may not only have the ability of improving the survivability in the soil environment, converting nitrogen and potassium in soil, improving the soil fertility, and simulating the crop growth, but also reduce the amount of chemical fertilizers, inhibit the repro- duction of germs, and achieve the three-in-one effect of increas- ing the production and income, inhibiting diseases, and improving the quality, so the input cost is reduced. 2. A microbial inoculant for composting livestock and poultry manure and a preparation method thereof, by adding the trichoderma harzianum, a cell wall degrading enzyme of pathogenic bacteria may be secreted, and the cell wall of the pathogenic bacteria may be destroyed, thereby the pathogenic bacteria are killed; by adding the paecilomyces lilacinus, it has the efficacy of preventing and controlling a plurality of nematodes; and by adding the bacillus coagulans, it has an anti-insect function to plants, thereby the immunity of crops is improved, and the disease resistance of the crops 1s strengthened.
FIG. 1 is a flow diagram of a preparation method of the pre- sent invention.
Embodiment 1
The present invention provides a technical scheme: a microbi- al inoculant for composting livestock and poultry manure, which is prepared from the following components in parts by mass: 2.0 parts of bacillus subtilis, 2.6 parts of azotobacter vinelandii, 1.8 parts of paecilomyces lilacinus, 3.3 parts of bacillus coagulans, 2.1 parts of trichoderma harzianum, 4.0 parts of streptomyces mi- 5 croflavus, 0.6 parts of trichoderma, 0.3 parts of organophosphorus bacteria, 0.3 parts of potassium bacteria and 0.5 parts of rhizo- bium astragali.
The streptomyces microflavus is mainly fermented from a lig- uid culture medium of soybean cake powder, glucose, a calcium hy- drochloride and a sodium chloride.
The content of the soybean cake powder is 3 g, the content of the glucose is 3 g, the content of the calcium hydrochloride is 0.5 g, and the content of the sodium chloride is 0.4 g.
The azotobacter vinelandii and the potassium bacteria are both cultured in an Ashby medium, the trichoderma is cultured in a potato sucrose medium, the organophosphorus bacteria are cultured in a lecithin medium, and the rhizobium astragali is cultured in a yeast juice sucrose medium.
A preparation method for the above microbial inoculant for composting the livestock and poultry manure includes the following steps: 81. strain culture: inoculating the bacillus subtilis, azoto- bacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, or- ganophosphorus bacteria, potassium bacteria and rhizobium astraga- li respectively in each culture medium for culturing;
S2. bacterial solution preparation: putting the various bac- terial strains cultured in the Step S1 into a drug bottle of a seed culture medium in a mode of slant surface inoculation, and using a liquid culture medium for culturing separately, to obtain bacterial solution of the bacterial strain; 33. bacterial solution mixing: putting all types of the bac- terial solution prepared in the Step S2 into a fermentation tank loaded with seeds, stirring and mixing, to obtain composite seed solution;
S4. compound bacterial solution preparation: inoculating all the seed solution in the Step S3 into a seed tank loaded with a fermentation culture medium for fermenting and culturing, to ob- tain compound bacterial solution; 85. microbial inoculant preparation: drying and powder- spraying the compound bacterial solution in the Step S4, to obtain a finished product microbial incculant; and
S6. packaging and storing: adding the finished product micro- bial inoculant in the Step S5 to a filling machine, packaging it into a container by using the filling machine, and putting it in a storage.
Embodiment 2
The present invention provides a technical scheme: a microbi- al inoculant for composting livestock and poultry manure, which is prepared from the following components in parts by mass: 2.5 parts of bacillus subtilis, 3.0 parts of azotobacter vinelandii, 2.1 parts of paecilomyces lilacinus, 3.6 parts of bacillus coagulans, 2.6 parts of trichoderma harzianum, 4.1 parts of streptomyces mi- croflavus, 0.7 parts of trichoderma, 0.35 parts of organophospho- rus bacteria, 0.35 parts of potassium bacteria and 0.7 parts of rhizobium astragali.
The streptomyces microflavus is mainly fermented from a lig- uid culture medium of soybean cake powder, glucose, a calcium hy- drochloride and a sodium chloride.
The content of the soybean cake powder is 3 g, the content of the glucose is 3 g, the content of the calcium hydrochloride is 0.5 g, and the content of the sodium chloride is 0.4 g.
The azotobacter vinelandii and the potassium bacteria are both cultured in an Ashby medium, the trichoderma is cultured in a potato sucrose medium, the organophosphorus bacteria are cultured in a lecithin medium, and the rhizobium astragali is cultured in a yeast juice sucrose medium.
A preparation method for the above microbial inoculant for composting the livestock and poultry manure is the same as Embodi- ment 1.
Embodiment 3
The present invention provides a technical scheme: a microbi- al inoculant for composting livestock and poultry manure, which is prepared from the following components in parts by mass: 3.0 parts of bacillus subtilis, 3.7 parts of azotobacter vinelandii, 2.4 parts of paecilomyces lilacinus, 4.0 parts of bacillus coagulans, 3.1 parts of trichoderma harzianum, 4.2 parts of streptomyces mi- croflavus, 0.8 parts of trichoderma, 0.4 parts of organophosphorus bacteria, 0.4 parts of potassium bacteria and 1.0 part of rhizobi- um astragali.
The streptomyces microflavus is mainly fermented from a liq- uid culture medium of soybean cake powder, glucose, a calcium hy- drochloride and a sodium chloride.
The content of the soybean cake powder is 3 g, the content of the glucose is 3 g, the content of the calcium hydrochloride is 0.5 g, and the content of the sodium chloride is 0.4 g.
The azotobacter vinelandii and the potassium bacteria are both cultured in an Ashby medium, the trichoderma is cultured in a potato sucrose medium, the organophosphorus bacteria are cultured in a lecithin medium, and the rhizobium astragali is cultured in a yeast juice sucrose medium.
A preparation method for the above microbial inoculant for composting the livestock and poultry manure includes the following steps:
Sl. strain culture: inoculating the bacillus subtilis, azoto- bacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, or- ganophosphorus bacteria, potassium bacteria and rhizobium astraga- li respectively in each culture medium for culturing;
S2. bacterial solution preparation: putting the various bac- terial strains cultured in the Step S1 into a drug bottle of a seed culture medium in a mode of slant surface inoculation, and using a liquid culture medium for culturing separately, to obtain bacterial solution of the bacterial strain;
S3. bacterial solution mixing: putting all types of the bac- terial solution prepared in the Step S2 into a fermentation tank loaded with seeds, stirring and mixing, to obtain composite seed solution;
S4. compound bacterial solution preparation: inoculating all the seed solution in the Step S3 into a seed tank loaded with a fermentation culture medium for fermenting and culturing, to ob-
tain compound bacterial solution; 35. microbial inoculant preparation: drying and powder- spraying the compound bacterial solution in the Step S4, to obtain a finished product microbial inoculant; and
S56. packaging and storing: adding the finished product micro- bial inoculant in the Step S5 to a filling machine, packaging it into a container by using the filling machine, and putting it in a storage.
I. Test method for antagonism of actinomyces microflavus: 1) a tube of the azotobacter vinelandii, the organophosphorus bacteria, the potassium bacteria, the rhizobium astragali and the trichoderma is taken each; 2) bacterial cells are washed out respectively with sterile water, 1 mm of bacterial suspension is absorbed each, mixed uni- formly with an uncured agar medium and poured into a culture dish; and 3) after being cured, fermentation broth is dipped with a circular filter paper sheet with a diameter of 1 cm and it sticks on the medium, after being placed at 26-28°C for 5-7 days, the size of an inhibition zone is observed.
Results are shown in the following table:
Bacterial strain Diameter of inhibition zone (mm)
Organophosphorus bacteria °o ae
Rhizobium astragali 0
From the test results in the above table, it may be seen that the actinomyces microflavus has the apparent inhibitory effect on the trichoderma, but has no inhibitory effect on the potassium bacteria, the organophosphorus bacteria and the rhizobium astraga- 1i, and not only has no inhibitory effect on the azotobacter vine- landii, but also causes the azotobacter vinelandii to generate a dense bacterial lawn with a thickness of 6 mm around the filter paper sheet, so it has the effect of stimulating growth.
It should be noted that, in this article, relationship terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not neces- sarily require or imply any such actual relationship or sequence existing between these entities or operations. In addition, terms "including", "containing" or any other variations thereof are in- tended to encompass non-exclusive inclusion, such that a process, a method, an object or a device including a series of elements in- cludes not only those elements, but also includes other elements that are not explicitly listed, or elements inherent to such a process, method, object or device.
Although the embodiments of the present invention are already shown and described, it may be understood by those skilled in the art that various changes, modifications, replacements and varia- tions may be made to these embodiments without departing from the principle and spirit of the present invention, and the scope of the present invention is defined by the appended claims and equiv- alents thereof.
Claims (1)
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| WO2017184810A1 (en) * | 2016-04-21 | 2017-10-26 | Koch Agronomic Services, Llc | Microbial inoculant compositions and uses thereof |
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