MX2020010393A - Proceso para producir una proteina de membrana. - Google Patents
Proceso para producir una proteina de membrana.Info
- Publication number
- MX2020010393A MX2020010393A MX2020010393A MX2020010393A MX2020010393A MX 2020010393 A MX2020010393 A MX 2020010393A MX 2020010393 A MX2020010393 A MX 2020010393A MX 2020010393 A MX2020010393 A MX 2020010393A MX 2020010393 A MX2020010393 A MX 2020010393A
- Authority
- MX
- Mexico
- Prior art keywords
- membrane protein
- membrane
- stationary phase
- host organism
- liquid fraction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se describe un proceso para la producción de una proteína de membrana que comprende las etapas de: expresar una proteína de membrana en un organismo huésped presente en un medio acuoso, liberar la proteína de membrana del organismo huésped, añadir una solución de detergente para solubilizar la proteína de membrana, recuperar una fracción líquida de la proteína de membrana solubilizada, someter la fracción líquida a cromatografía para unir o retener la proteína de membrana en una fase estacionaria, y eluir la fase estacionaria con un tampón de elución para producir la proteína de membrana. Los procesos puede producir cantidades relativamente grandes de proteínas de membrana de manera eficiente sin comprometer la calidad del producto final.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA201870202 | 2018-04-06 | ||
| PCT/EP2019/058609 WO2019193139A1 (en) | 2018-04-06 | 2019-04-05 | Process for producing a membrane protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020010393A true MX2020010393A (es) | 2020-10-22 |
Family
ID=66102107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020010393A MX2020010393A (es) | 2018-04-06 | 2019-04-05 | Proceso para producir una proteina de membrana. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20210147477A1 (es) |
| EP (1) | EP3774842A1 (es) |
| JP (2) | JP2021519800A (es) |
| KR (1) | KR20210003782A (es) |
| CN (1) | CN112189016A (es) |
| AU (1) | AU2019249933A1 (es) |
| BR (1) | BR112020020379A2 (es) |
| CA (1) | CA3095674A1 (es) |
| IL (1) | IL277565A (es) |
| MX (1) | MX2020010393A (es) |
| SG (1) | SG11202009521YA (es) |
| WO (1) | WO2019193139A1 (es) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5468847A (en) * | 1994-03-10 | 1995-11-21 | Minnesota Mining And Manufacturing Company | Method of isolating and purifying a biomacromolecule |
| WO2008073620A2 (en) * | 2006-11-02 | 2008-06-19 | Neose Technologies, Inc. | Manufacturing process for the production of polypeptides expressed in insect cell-lines |
| DK177144B1 (en) | 2009-06-19 | 2012-02-06 | Aquaporin As | A liquid membrane suitable for water extraction |
| TWI561294B (en) | 2011-09-21 | 2016-12-11 | Univ Nanyang Tech | Aquaporin based thin film composite membranes |
| US9334319B2 (en) * | 2012-04-20 | 2016-05-10 | Abbvie Inc. | Low acidic species compositions |
| GB201300465D0 (en) | 2013-01-11 | 2013-02-27 | Aquaporin As | A hollow fiber module having tfc-aquaporin modified membranes |
| EP3413998B1 (en) | 2016-02-08 | 2024-05-15 | Aquaporin A/S | Separation membranes comprising self-assembled nanostructures with aquaporin water channels and methods of making and using them |
-
2019
- 2019-04-05 MX MX2020010393A patent/MX2020010393A/es unknown
- 2019-04-05 SG SG11202009521YA patent/SG11202009521YA/en unknown
- 2019-04-05 CN CN201980023803.XA patent/CN112189016A/zh active Pending
- 2019-04-05 JP JP2020554416A patent/JP2021519800A/ja active Pending
- 2019-04-05 EP EP19716391.8A patent/EP3774842A1/en active Pending
- 2019-04-05 KR KR1020207031915A patent/KR20210003782A/ko not_active Application Discontinuation
- 2019-04-05 WO PCT/EP2019/058609 patent/WO2019193139A1/en active Application Filing
- 2019-04-05 CA CA3095674A patent/CA3095674A1/en active Pending
- 2019-04-05 BR BR112020020379-3A patent/BR112020020379A2/pt unknown
- 2019-04-05 AU AU2019249933A patent/AU2019249933A1/en active Pending
- 2019-04-05 US US17/044,910 patent/US20210147477A1/en active Pending
-
2020
- 2020-09-23 IL IL277565A patent/IL277565A/en unknown
-
2023
- 2023-10-02 JP JP2023171068A patent/JP2023182713A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| SG11202009521YA (en) | 2020-10-29 |
| JP2023182713A (ja) | 2023-12-26 |
| AU2019249933A1 (en) | 2020-10-22 |
| JP2021519800A (ja) | 2021-08-12 |
| WO2019193139A1 (en) | 2019-10-10 |
| RU2020136185A (ru) | 2022-05-06 |
| CA3095674A1 (en) | 2019-10-10 |
| BR112020020379A2 (pt) | 2021-01-12 |
| US20210147477A1 (en) | 2021-05-20 |
| IL277565A (en) | 2020-11-30 |
| EP3774842A1 (en) | 2021-02-17 |
| CN112189016A (zh) | 2021-01-05 |
| KR20210003782A (ko) | 2021-01-12 |
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