KR102482128B1 - Compositions containing new methylsulfonylmethane(MSM) with increased arthritis treatment effect and the process of manufacture thereof - Google Patents

Abstract

The present invention is to prepare a composition by additionally adding methylsulfonylmethane (MSM), seaweed calcium and herbal extracts that help joint and cartilage health, as well as vitamin C and PDRN that increase collagen biosynthesis, thereby reducing the weakened joint and cartilage health. It is hoped that this will help in normalization.

Description

Compositions containing new methylsulfonylmethane (MSM) with increased arthritis treatment effect and the process of manufacture thereof}

The present invention is a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis, and more specifically, methylsulfonylmethane (MSM), seaweed calcium and several herbal medicines that can help joint and cartilage health In a composition containing methylsulfonylmethane (MSM), which can help normalize weakened bones, cartilage, ligaments and tendons by helping collagen biosynthesis by preparing a composition to which vitamin C and PDRN necessary for collagen biosynthesis are added as well as extracts. it's about

In the human body, a joint is a part where two bones are connected and is surrounded by articular cartilage. Bone is a hard tissue in which calcium and phosphorus are calcified and plays an important role in supporting and protecting the body. It can be called a joint or a bone. However, modern people are overloaded by various causes such as lack of calcium and protein, imbalance of hormones, decrease in physical activity, and frequent intake of processed foods, which can harm joint or bone health.

Arthritis is a disease that causes inflammation and pain in joints, and can be mainly divided into osteoarthritis (degenerative arthritis) and rheumatoid arthritis. Since each disease has a different pathogenesis and symptoms and different treatment, an accurate diagnosis is essential once a joint problem occurs. Usually, arthritis is treated with oral medications, but there is still no way to cure arthritis with short-term use. To maintain and strengthen joint function, as well as medication, continuous exercise in daily life or eating health functional foods are also available. .

Methylsulfonylmethane (MSM) is an essential component of cartilage connective tissue such as bone and collagen. Cartilage fixes joints so that they do not shake and smoothes joint movement. In addition, it absorbs and disperses shock to prevent joint and bone damage. Collagen increases skin elasticity as well as muscle strength, and is also a major component of cartilage.

The Ministry of Food and Drug Safety recognized MSM as a functional raw material for health functional foods that can help joint and cartilage health as an organosulfur compound containing sulfur. Intake of MSM helps to manage joint health, such as strengthening cartilage function by enriching collagen in the human body and relieving joint inflammation and pain caused by it. In addition, it increases cell permeability to discharge toxins and waste products, has antioxidant effects, blocks pain transmission nerves, prevents nerve cell damage, and has effects such as repair and treatment of somatic tissue.

In addition, Polydeoxyribonucleotide (PDRN) is a cell regeneration promoting substance. It is a low-molecular DNA complex that selectively acts on adenosine A2 receptors when administered into the body and selectively exhibits anti-inflammatory effects, cell proliferation, and tissue regeneration effects on damaged areas. It shows effective tissue regeneration effect.

Taking the prior art related to the addition of MSM to food as an example, Korean Registered Patent Registration No. 10-1594137 discloses a health functional food composition that helps joint health, but the above patent discloses MSM and MSM without additional seaweed calcium addition. Herbal medicine extract is mixed, homogenized, and then tableted to produce a health functional food that helps joint health, which is different from the present invention in which a liquid composition containing MSM, seaweed calcium, and herbal medicine extract is prepared.

In addition, Korean Patent Publication No. 10-2014-0116988 discloses a pharmaceutical composition for the prevention and treatment of rheumatoid arthritis and lipid metabolism-related diseases containing methylsulfonylmethane, but the patent is a separate herbal extract MSM, vitamin B6 hydrochloride, folic acid, and coral powder are mixed without addition and then tableted, which is different from the present invention in which a liquid composition containing MSM, seaweed calcium, and herbal extract is prepared.

Accordingly, while the present inventors were conducting research to develop a functional food containing MSM as an active ingredient, the composition containing MSM, seaweed calcium, and herbal extracts could not only prevent and improve arthritis, but also help collagen biosynthesis. The present invention was completed by finding that it can help normalize weakened bones, cartilage, ligaments and tendons, can be easily ingested in a liquid formulation, and has an improved absorption rate.

Korean Patent Registration No. 10-1594137, Health functional food composition that helps joint health, registered on February 04, 2016. Korean Patent Publication No. 10-2014-0116988, Pharmaceutical composition for the prevention and treatment of rheumatoid arthritis and lipid metabolism-related diseases containing methylsulfonylmethane, published on October 7, 2014.

An object of the present invention is to provide a new composition containing methylsulfonylmethane (MSM), seaweed calcium, herbal extract, vitamin C and PDRN with increased therapeutic effect on arthritis.

In addition, the present invention is to provide a method for preparing the above composition containing new methylsulfonylmethane (MSM), seaweed calcium, crude drug extract, vitamin C and PDRN with increased therapeutic effect on arthritis.

In addition, the present invention is to provide a health functional food using the composition containing new methylsulfonylmethane (MSM), seaweed calcium, herbal extract, vitamin C and PDRN with increased therapeutic effect on arthritis.

The present invention comprises the steps of heating and extracting a plant mixture raw material and adding purified water corresponding to 8 times the weight of the raw material to a solid content of 2% or more (first step);

Preparing a mixed plant extract by first filtration after the extraction (second step);

Mixing the mixed plant extract prepared in step 2 with MSM and seaweed calcium according to the mixing ratio (third step);

Sterilizing the mixture prepared in step 3 at 90±5° C. for 60±5 minutes (step 4);

Mixing the sterilized mixture with vitamin C and PDRN according to the mixing ratio (step 5);

Secondary filtering the mixture prepared in step 5 (step 6); and

Packing the secondary filtered mixture in step 6 (step 7); It is possible to provide a method for producing the composition that helps joint and cartilage health by increasing collagen biosynthesis comprising a.

In addition, the present invention can provide a composition containing methylsulfonylmethane (MSM), seaweed calcium, herbal extract, vitamin C and PDRN, which help joint and cartilage health by increasing collagen biosynthesis.

In addition, the present invention can provide a health functional food using the composition that helps joint and cartilage health by increasing collagen biosynthesis.

Hereinafter, the present invention will be described in detail.

In the first step, the plant mixture is shovel, malt, gujeolcho, mint, dermis, cine, hawthorn, yams, quince, astragalus, succulent rehmannia, licorice, mokryeong, cinnamon, cinnamon, ginseng, cnidium, gourd peony, angelica and pine needles It can be selected from the group consisting of.

In the second step, filtration was performed using a filter having an average pore size of 50 μm to maximize the separation of debris.

In the third step, the mixed plant extract, MSM, and seaweed calcium may be mixed in a weight ratio of 100: 0.1 to 10: 0.1 to 10, respectively, and preferably in a weight ratio of 100: 0.6 to 2.5: 1.1 to 4.6, More preferably, they may be mixed in a weight ratio of 100: 0.9 to 1.5: 1.4 to 2.8.

The mixture sterilized in the fifth step, vitamin C and PDRN may be mixed in a weight ratio of 100: 0.01 to 5: 0.01 to 5, respectively, preferably in a weight ratio of 100: 0.01 to 0.05: 0.01 to 0.05, More preferably, it may be mixed in a weight ratio of 100: 0.01 to 0.02: 0.01 to 0.02.

Also, in the fifth step, fructooligosaccharides may be added to improve taste.

In the sixth step, filtration was performed using a filter having an average pore size of 50 μm to maximize the separation of debris.

The pharmaceutical composition according to the present invention may be formulated in a suitable form together with a generally used pharmaceutically acceptable carrier. "Pharmaceutically acceptable" refers to a composition that is physiologically acceptable and does not usually cause an allergic reaction such as gastrointestinal disorder, dizziness, or similar reaction when administered to humans. In addition, the composition may be formulated and used in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and sterile injection solutions according to conventional methods.

Carriers, excipients and diluents that may be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum arabic, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl paraoxybenzoate, propyl paraoxybenzoate, talc, magnesium stearate, and mineral oil, but is not limited thereto. When formulated, it is prepared using diluents or excipients such as commonly used fillers, stabilizers, binders, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient for the extract of the present invention, for example, starch, microcrystalline cellulose, sucrose or lactose, It is prepared by mixing low-substituted hydroxypropyl cellulose, hypromellose, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solutions for oral use, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerol, gelatin, and the like may be used. To formulate a formulation for parenteral administration, the composition is sterilized and/or dissolved in water together with adjuvants such as preservatives, stabilizers, hydrating or emulsifying accelerators, salts and/or buffers for adjusting osmotic pressure, and other therapeutically useful substances. It can be mixed to prepare a solution or suspension, which can be prepared in ampoule or vial unit dosage form.

In another aspect, the present invention relates to a health functional food for preventing or improving arthritis, comprising a new composition containing methylsulfonylmethane (MSM) as an active ingredient.

The health functional food refers to food manufactured or processed using raw materials or ingredients having useful functionalities, and includes forms such as tablets, capsules, pills, or liquids, and includes, for example, health supplements, functional foods, and nutritional supplements. , supplements, and the like.

According to the present invention, a composition is prepared by separately adding methylsulfonylmethane (MSM), seaweed calcium and herbal extracts that help joint and cartilage health, as well as vitamin C and PDRN that increase collagen biosynthesis, thereby helping to treat arthritis. hope to be able to give

1 is a flow chart sequentially showing the manufacturing process of a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the disclosure herein is provided so that it will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art.

<Example 1. Preparation of composition 1 containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis>

Compositions containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis were prepared under the following conditions: Example 1-1 to Example 1-3.

The present invention is a plant mixture selected from equal amounts of shovel, malt, gujeolcho, peppermint, dermis, cine, hawthorn, oxtail, quince, astragalus, sukjihwang, licorice, mokryeong, gyeji, cinnamon, sasam, cnidium, basil peony, angelica and pine needles Adding raw material and adding purified water corresponding to 8 times the weight of the raw material and extracting by heating at 90 ° C or higher so that the solid content is 2% or more (first step);

Preparing a mixed plant extract by primary filtration using a filter having an average pore size of 50 μm after the extraction (second step);

Mixing the mixed plant extract, MSM, and seaweed calcium prepared in step 2 in parts by weight of Table 1 below (third step);

Sterilizing the mixture prepared in step 3 at 90° C. for 60 minutes (step 4);

mixing the sterilized mixture prepared in step 4, vitamin C, and PDRN in parts by weight of Table 1 below (step 5);

Secondary filtration of the mixture prepared in step 5 using a filter having an average pore size of 50 μm (step 6); and

Packing the secondary filtered mixture in step 6 (step 7); Compositions containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis were prepared through the process of Example 1-1 to Example 1-3.

<Comparative Example 1. Preparation of Comparative Composition 1>

Composition Comparative Example 1-1 to Comparative Example 1-6 were prepared under the following conditions.

In addition, MSM, seaweed calcium, vitamin C and PDRN were added or not added as summarized in Table 2 below.

Add the same amount of plant mixture selected from shovel, malt, gujeolcho, mint, dermis, cine, hawthorn, oxtail, quince, astragalus, sujihwang, licorice, mokryeong, cinnamon, cinnamon, sasam, cnidium, gourd peony, angelica and pine needles Extracting by adding purified water corresponding to 8 times the weight of the raw material and heating at 90 ° C or higher so that the solid content is 2% or more (first step);

Preparing a mixed plant extract by primary filtration using a filter having an average pore size of 50 μm after the extraction (second step);

Mixing the mixed plant extract, MSM, and seaweed calcium prepared in step 2 in parts by weight shown in Table 2 below (third step);

Sterilizing the mixture prepared in step 3 at 90° C. for 60 minutes (step 4);

Mixing the sterilized mixture prepared in step 4, vitamin C, and PDRN in parts by weight of Table 2 below (step 5);

Secondary filtration of the mixture prepared in step 5 using a filter having an average pore size of 50 μm (step 6); and

Packing the secondary filtered mixture in step 6 (step 7); Through the process of Comparative Examples 1-1 to Comparative Examples 1-6 were prepared as comparative compositions.

<Test Example 1.>

Compositions containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis obtained in Example 1 and Comparative Example 1 were administered twice a day to 50 people who felt discomfort due to swelling and pain in the joint area. (Morning and evening) 70 ml each, and 45 days and 90 days after intake, respectively, the joint swelling and pain relief effects were compared and evaluated with those before taking, and the results are shown in Table 3.

From Table 3, as the evaluation criterion for the swelling and pain relief effect of the joint, 'excellent' means 'reduction of swelling and pain in the joint by 80% or more compared to before ingestion of the composition', and 'good' is 'compared to before ingestion of the composition. It means that swelling and pain in the area are reduced by 60% or more, 'normal' means 'swelling and pain in the joint area reduced by about 40% compared to before taking the composition', and 'weak' means 'joint joint compared to before taking the composition' It means that swelling and pain in the area are reduced by about 20%', and 'no' means 'no change in swelling and pain in the joint area compared to before taking the composition and before treatment'.

In addition, from Table 3, the compositions Examples 1-1 to 1-3 prepared through the process of Example 1 of the present invention increase collagen biosynthesis to help joint and cartilage health. And the rate of evaluating the pain relief effect as excellent or good exceeds 80%, and there is no person who evaluates the effect as weak or absent, so it can be confirmed that it is effective for various people due to various causes.

On the other hand, in the case of the composition (Comparative Example 1-1) containing vitamin C and PDRN within the preferred range but an amount of MSM and seaweed calcium that is below the preferred range, swelling of the joint due to the insufficient content of MSM and seaweed calcium. And the proportion of subjects who evaluated that they felt a slight relief of pain was high. However, some of the subjects who did not feel the relief effect due to long-term use also felt the relief effect.

In addition, in the case of the composition (Comparative Example 1-2) containing vitamin C and PDRN within the preferred range, but containing an excess amount of MSM and seaweed calcium outside the preferred range, excellent effects were observed in relieving swelling and pain in the joint area. There were some subjects, but there were also subjects who suffered from stomach pain and digestive disorders due to the side effects of excessive intake of MSM and seaweed calcium.

In addition, in the case of a composition containing only MSM and seaweed calcium in a preferred range without vitamin C and PDRN at all (Comparative Examples 1-3), vitamin C and PDRN within a preferred range while containing the same amount of MSM and seaweed calcium Compared with the contained Example 1-1, there was a tendency for the joint swelling and pain relieving effect to be significantly lowered. It is well known that the composition containing MSM and seaweed calcium is effective in relieving swelling and pain in the joint area. If vitamin C or PDRN is added in a preferred range, it can be expected that the relief effect on joint swelling and pain can be increased.

In addition, in the case of the composition containing MSM, seaweed calcium and vitamin C within the preferred range but not adding PDRN (Comparative Examples 1-4), collagen biosynthesis even though MSM, seaweed calcium and vitamin C were added in the preferred range. It is difficult to expect a synergistic effect because both vitamin C and PDRN, which are helpful for skin healing, are not added. However, some of the subjects who did not feel the relief effect due to long-term use also felt the relief effect.

In addition, in the case of compositions containing MSM, seaweed calcium and PDRN within the preferred range but no vitamin C added (Comparative Examples 1-5), even though MSM, seaweed calcium and PDRN were added in the preferred range, collagen biosynthesis was not affected. It is difficult to expect a synergistic effect because both helpful vitamin C and PDRN are not added, and the ratio of subjects who actually felt an excellent effect in relieving joint swelling and pain was low. However, some of the subjects who did not feel the relief effect due to long-term use also felt the relief effect.

In addition, in the case of the composition (Comparative Example 1-6) containing MSM and seaweed calcium within the preferred range but containing an excess amount of vitamin C and PDRN out of the preferred range, vitamin C and PDRN do not have side effects even when consumed in excess. There are subjects who felt an excellent effect in relieving swelling and pain in the joint area, but the increase in unit price due to the excessive content of vitamin C and PDRN is much larger than the increase in efficacy, so there is a tendency not to be suitable.

In conclusion, it is known that the composition containing MSM and seaweed calcium is effective in relieving swelling and pain in the joint area, but in the case of the composition containing an excess amount of MSM and seaweed calcium outside the preferred range, swelling and pain in the joint area It is true that there is a limit to the content of MSM and seaweed calcium in the composition because some subjects may experience gastrointestinal pain or digestive disorders as a side effect of excessive intake of MSM and seaweed calcium. In addition, from the test results of Comparative Examples 1-4 and 1-5, it can be confirmed that a synergistic effect exists in collagen biosynthesis when both vitamin C and PDRN are added.

From the above results, if vitamin C and PDRN, which help in collagen biosynthesis, are added in a preferred range to a composition containing MSM and seaweed calcium to relieve swelling and pain in the joint area, the relief effect on swelling and pain in the joint area can be significantly increased. can look forward to

<Formulation Example 1. Preparation of a health food containing a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis>

1-1. Manufacture of health food

41 kg of the composition containing methylsulfonylmethane (MSM) prepared in Example 1, 20 kg of brewer's yeast, 18.4 kg of soybean protein isolation, 10 kg of polydextrose, 8 kg of indigestible dextrin, 0.2 kg of vitamin B1 hydrochloride, 0.1 kg of vitamin B2, and vitamin B6 A health food was prepared by mixing and processing 0.1 kg of hydrochloride, 0.5 kg of vitamin C, 0.5 kg of vitamin E, 1 kg of seaweed calcium, and 0.2 kg of zinc oxide.

1-2. Manufacture of functional food

81 kg of the composition containing methylsulfonylmethane (MSM) prepared in Example 1, 1 kg of coenzyme Q10, 2 kg of grape seed extract powder, 2 kg of vitamin C, 2 kg of vitamin E, 10 kg of erythritol, and 2 kg of malic acid were mixed and processed to prepare a functional food did

1-3. Manufacture of multi-category foods

14 kg of the composition containing methylsulfonylmethane (MSM) prepared in Example 1, 12 kg of mate extract, 10 kg of green tea extract powder, 10 kg of prune juice powder, 10 kg of inulin, 20 kg of erythritol, and 24 kg of lactose were mixed and processed to prepare tea products. did

<Formulation Example 1. Preparation of tablets>

20 g of the composition containing methylsulfonylmethane (MSM) prepared in Example 1 of the present invention was mixed with 175.9 g of lactose, 180 g of potato starch, and 32 g of colloidal silicic acid, respectively. After adding 10% gelatin solution to this mixture, it was pulverized and passed through a 14 mesh sieve. It was dried and the mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.

<Formulation Example 2. Preparation of capsules>

After mixing 100 mg of the composition containing methylsulfonylmethane (MSM) prepared in Example 1 of the present invention, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate, the above components were prepared according to a conventional capsule preparation method. were mixed and filled into gelatin capsules to prepare capsules.

<Formulation Example 3. Preparation of liquid formulation>

Purified water was added to 65 ml of the composition containing methylsulfonylmethane (MSM) prepared in Example 1 of the present invention and 100 g of oligosaccharide to make a total of 70 ml, and then the above components were mixed according to a conventional liquid preparation method. Next, the pouch was filled and sterilized to prepare a liquid formulation.

Claims (8)

Plant mixture consisting of shovel, malt, gujeolcho, peppermint, dermis, cine, hawthorn, oxtail, quince, astragalus, succulent rehmannia, licorice, mokryeong, cinnamon, cinnamon, sasam, cnidium, basil peony, angelica and pine needles was added and the original Adding purified water corresponding to 8 times the weight and heating and extracting the solid content to 2% or more (first step);
Preparing a mixed plant extract by first filtration after the extraction (second step);
Mixing the mixed plant extract prepared in step 2 with MSM and seaweed calcium according to the mixing ratio (third step);
Sterilizing the mixture prepared in step 3 at 90±5° C. for 60±5 minutes (step 4);
Mixing the sterilized mixture prepared in the fourth step with vitamin C and PDRN (Polydeoxyribonucleotide) according to the mixing ratio (5th step);
Secondary filtering the mixture prepared in step 5 (step 6); and
Packing the secondary filtered mixture in step 6 (step 7);
Method for producing a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis, characterized in that it comprises a. delete According to claim 1,
In the third step, MSM is contained in the range of 0.1 to 10 parts by weight based on 100 parts by weight of the plant mixture extract. Method for producing a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis . According to claim 1,
In the third step, the seaweed calcium is contained within the range of 0.1 to 10 parts by weight based on 100 parts by weight of the plant mixture extract Preparation of a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis method. According to claim 1,
In the fifth step, vitamin C is contained in the range of 0.01 to 5 parts by weight based on 100 parts by weight of the sterilized mixture Preparation of a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis method. According to claim 1,
In the fifth step, PDRN is contained within the range of 0.001 to 5 parts by weight based on 100 parts by weight of the sterilized mixture. Method for producing a composition containing new methylsulfonylmethane (MSM) with increased therapeutic effect on arthritis . A pharmaceutical composition for the treatment of arthritis containing methylsulfonylmethane (MSM), characterized in that it is prepared by the method of any one of claims 1 and 3 to 6. A health functional food for improving arthritis containing methylsulfonylmethane (MSM), characterized in that it is prepared by the method of any one of claims 1 and 3 to 6.

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