KR102185403B1 - Natural Shampoo by using of Artemisia sieversiana Willd with the function of hair loss prevention and hair growth promotion and Manufacturing Method thereof - Google Patents

Abstract

The present invention relates to a natural shampoo having an anti-androgen, anti-aromatase, hair root reinforcement, antibacterial effect, and oxidizing effect, and a natural shampoo having a function of promoting hair growth and prevention of hair growth using Daeja Lake.
The shampoo for preventing hair loss and promoting hair growth according to the present invention is characterized in that it contains Daejaho extract as an active ingredient.

Description

[Natural Shampoo by using of Artemisia sieversiana Willd with the function of hair loss prevention and hair growth promotion and Manufacturing Method thereof]

The present invention relates to a natural shampoo having an anti-androgen, anti-aromatase, hair root reinforcement, antibacterial effect, and oxidizing effect, and a natural shampoo having a function of promoting hair growth and prevention of hair growth using Daeja Lake.

The occurrence of hair loss is divided into environmental and genetic factors such as fatigue, stress, disease, and environmental changes such as drugs. In the case of male hair loss, genetic factors and androgen hormone are reported as important factors, and it is estimated that some of female-type hair loss occurs in the same path as male-type hair loss, but clinically there is a difference in the pattern. Because females secrete less testosterone hormone and produce less DHT, their hair loss rate is 50% less than that of males, and androgen hair loss may occur in their 20s. When female hormones decrease, hair follicles weaken and aromatase causes the crown to fall out or Widens. As the scalp becomes thinner and pores cannot be opened and closed, sebum or sweat cannot be discharged to the outside, causing damage to the hair follicles.Therefore, it is necessary to always clean the scalp by removing dead dead skin cells and impurities, and open pores and sweat lines (pores) to supply nutrition. In addition, hair follicle reinforcement and dermal cell proliferation induces hair follicle cell activation to induce a hair loss inhibitory effect.

Accordingly, the present inventors have investigated the anti-androgen and anti-aromaase activity of the extract of Daeja Lake (white tiger), inhibiting the activity of anti-aromaase enzymes, cell proliferation of dermal papilla cells and the effect of strengthening hair follicle cells, the mechanism of cell signaling and in The present invention was completed by confirming the efficacy of hair growth in vivo.

The problem to be solved by the present invention is to provide a natural shampoo having an anti-androgen, anti-aromatase, hair root reinforcement, antibacterial effect, and a bio-friendly hair loss prevention and hair growth promotion function through an oxidizing effect and a method of manufacturing the same using a large arc I have to.

In addition, the natural shampoo using the Daeja Lake is another subject to provide dog shampoo, feminine cleanser, body washer, body lotion, and hair conditioner.

In order to achieve the above object, the shampoo for preventing hair loss and promoting hair growth according to the present invention is characterized in that it contains Daejaho extract as an active ingredient.

Preferably, the Daejaho extract is characterized in that it is used together with lotus leaves, cypress, cypress, black beans, eoseongcho, green tea, and machihyeon.

Preferably, the solid shampoo is characterized in that it is used in combination with Greater Jalanica extract, coconut oil, palm o'nil, olive oil, camellia oil, castor oil and noni.

Preferably, lotus leaves, cypress, cypress, black beans, Eoseongcho, green tea, and machihyeon are mixed in a weight ratio of 2 to 3, based on 100 weight of the Daejaho extract.

The shampoo containing Daeja Lake of the present invention is anti-androgen and anti-aromaase enzyme activity inhibition, cell proliferation of dermal papilla cells and hair follicle cell strengthening efficacy, cell signaling mechanism and in vivo Using Daeja Lake, which has hair growth effect, has the effect of having a bio-friendly hair loss prevention and hair growth promotion function.

In addition, the natural shampoo using Daejaho is effective in protecting hair and moisturizing the skin by using dog shampoo, feminine cleanser, body washer, and body lotion.

In addition, even when used for a long time, there are no side effects to the human body, and it prevents hair loss and has an excellent hair growth effect.

1 is a graph of cell proliferation efficacy in human hair follicle dermal papilla cells of Daejaho extract according to the present invention.
Figure 2 is a graph confirming the expression of VEGF, bFGF, and EGF for the growth proliferation mechanism of the Daejaho extract according to the present invention [(A) ELISA assay (B) Western blot assay]
Figure 3 is an analysis and quantification graph of the testosterone, reductase and aromatase activity inhibitory effect of the extract according to the present invention at the RNA level.
Figure 4a is a graph (MTT assay 72h analysis) of the keratinocyte proliferation inhibition rate of the Daejaho extract according to the present invention.
Figure 4b is a graph (MTT assay 48h and 92h analysis) of the keratinocyte proliferation inhibition rate of the Daejaho extract according to the present invention.
Figure 5 is a graph measuring the reduction rate of inflammatory mediators (TNF-α, PGE2) of the Daeja Lake extract according to the present invention.
Figure 6 is a graph measuring the reduction rate of inflammatory cytokines (IL-1b, IL-6, IL-8) and chemokines (MCP-1) of Daeja Lake extract according to the present invention.
7 is a graph of the signaling mechanism of anti-inflammatory and antioxidant efficacy of Daeja Lake of the Daeja Lake extract according to the present invention.
Figures 8a, 8b is a phototrichogram image of the test group using a shampoo containing a large letter (Example) and a shampoo not containing the large letter (Comparative Example) according to the present invention.
9A and 9B are graphs of changes in the number of hairs of a test group using a shampoo containing a large letter (Example) and a shampoo not containing a large letter (Comparative Example) according to the invention.

Hereinafter, the present invention will be described in detail through preferred embodiments. However, these embodiments should be understood as describing the present invention to better understand the present invention, and the terms and words used are not to be construed as being limited to a conventional or dictionary meaning, and the inventor is the best in his own invention. Based on the principle that the concept of terms can be appropriately defined in order to describe in a method, it should be interpreted as a meaning and concept consistent with the technical idea of the present invention, and the present invention should not be construed as limiting it.

<Example 1: Confirmation of large character arc characteristics>

The dried Daeja Lake was cut into a size of 3-7 cm, and after mixing 20-30 L of purified water per 1 kg of Daeja Lake, it was treated with hot water to prepare a Daeja Lake extract. At this time, if the ratio of purified water is mixed with less than 20L, the hot water extraction process may not be performed for a sufficient time, and if the ratio of purified water is mixed with more than 30L, it takes too much time to extract hot water and sufficiently concentrate. there is a problem.

1) Confirmation of acute toxicity

To investigate the toxicity of Daeja Lake and confirm the lethal dose, the toxicity was tested by oral administration of Daeja Lake to male and female Spradue-Dawley (SD) rats. After single oral administration at doses of 0, 500, 1000, and 2000 mg/kg to 10 mice per group (5 mice each), mortality, general symptoms, and weight change were observed for 14 days. In addition, after the end of the observation period, an autopsy was performed to observe the gross findings. As a result of the experiment, no dead animals were observed in the Daejaho administration group, and no specific changes were observed in general symptoms, weight change and autopsy findings.

Male ICR mice were fasted from the evening before the experiment to facilitate absorption of the drug, and groups were divided according to their body weight on the day of the experiment, and Daejaho was orally administered at a concentration of 5g/kg per body weight and observed for abnormalities in behavior for 4 hours. . As a result of the experiment, no dead animals were observed even at a high concentration of 5g/kg of Daeja Lake, and no specific changes were observed in general symptoms, weight change and autopsy findings.

2) Repeated dose toxicity confirmation

Male ICR mice were fasted from the evening before the experiment to facilitate absorption of the drug, and the group was divided according to their weight on the day of the experiment, and Daejaho extract was orally administered at a concentration of 2g/kg per body weight, and there was no abnormality in behavior for more than 4 hours. Was observed and the drug was repeatedly administered orally for 5 days. In case of occurrence of mortality, an autopsy was performed immediately, and even if no mortality occurs, anesthesia was performed with ether the next day of drug administration, and an autopsy was performed. Compared with the control group, the presence of abnormalities in the organs was visually observed.

As a result of the experiment, Daejaho extract did not show any mortality in the acute toxicity test and repeated dose toxicity test, and no side effects or organ abnormalities were observed during autopsy. It is judged not.

3) Confirmation of cell proliferation

The presence or absence of cell proliferation efficacy of human epithelial keratinocytes and fair follicle dermal papilla cells against the extract of Daejaho (Baekhoe) was analyzed. First, human epidermal keratinocytes and human hair follicle dermal papilla cells were purchased from Abm (Richmond British columbia, Canada), and 100 U/ml penicillin-100 U/ml streptomycin and 10% fetal bovine serum (FBS, Invitrogen) in DMEM culture medium. And incubated in a 5% CO2 incubator. After dispensing the cultured cells into a 96 well plate, when 90% of the cells are cultured, Daejaho (AS) and Modam Korea's Daejaho extract (P-AS) were treated by concentration and cultured for 72 hours, followed by BrdU asaay. Was carried out.

As a result of the experiment, as shown in Fig. 1, in human fair follicle dermal papilla cells, Daejaho (AS) and Daejaho prescription (P-AS) were concentration-dependently 23% at 250 μg/mL, 500 μg/mL, and 1000 μg/mL. Cell proliferation increased by ~55%.

4) Study on the cell proliferation mechanism of Daeja Lake (AS)

In order to investigate the mechanism of cell proliferation in human fair follicle dermal papilla cells, VEGF 50 ng/mL, bFGF 50 ng/mL, and EGF 20 ng/mL when cultured cells were dispensed into 6 well plates and then cultured for 90%. After the first treatment for 2 hours, Daeja Lake 62.5, 250 μg/mL, and 500 μg/mL were treated and incubated in a CO ₂ incubator for 48 hours. After cultivation, the expression levels of VEGF, bFGF and EGF were confirmed from the culture supernatant by ELISA, and proteins were extracted from the cells and protein expression was confirmed by the Western method. As a result of the experiment, as shown in Fig. 2, the Daejaho extract significantly increased the expression of VEGF at 250 μg/mL and 500 μg/mL, and the expression of bFGF significantly increased at 500 μg/mL. EGF expression was significantly increased at 250 μg/mL and 500 μg/mL, confirming that cell proliferation in human fair follicle dermal papilla cells was due to the promotion of expression of VEGF, bFGF and EGF growth factors.

5) Testosterone, reductase and aromatase activity of Daeja Lake (AS)

In order to confirm the inhibition of testosterone, reductase and aromatase-1 in human fair follicle dermal papilla cells, the cells were aliquoted into 6 well plates, and when 90% of the cells were cultured, 20 ng/mL of EGF was first treated for 2 hours each. Daejaho 62.5, 250 μg/mL, and 500 μg/mL were treated and incubated in a CO ₂ incubator for 48 hours. After cultivation, total RNA was extracted from the cells, and the level of RNA expression was confirmed by RT-PCR method. As a result of the experiment, as shown in FIG. 3, the Daejaho extract reduced the mRNA expression of testosterone, reductase, and aromatase-1 at 62.5 μg/mL, 250 μg/mL, and 500 μg/mL.

6) Anti-inflammatory and antioxidant efficacy in human keratinocytes of Daejaho (AS)

6-1) keratinocyte proliferation inhibition rate experiment

After dispensing into a 96-well plate so that the number of cells per well was 1×10 ^{5, the} cells were cultured to be 90% confluent in a CO ₂ incubator. Each well was washed twice with DMEM medium to remove non-adherent cells, and then Daeja Lake was diluted by 0-500 μg/mL concentration. After 100 μL of Daeja Lake was added to each well, 1 μg/mL of LPS and 1 ng/mL of IFN-γ were added to stimulate the cells, followed by incubation in a CO ₂ incubator for 72 hours. The macrophage proliferation rate was tested in the same manner using the MTS, which measures the splenocyte proliferation ability described above. As a result of the experiment, as shown in Fig. 4a, it can be confirmed that cell proliferation was inhibited by about 30% in the inflammatory keratinocyte extract 62.5 μg/mL in the inflammatory keratinocyte, and inhibited cell proliferation from 250 μg/mL to 69.1%. there was. In addition, as shown in Fig. 4b, it was confirmed that the excellent inhibitory effect was up to 92 hours even in the time-dependent growth inhibition experiment.

6-2) Measurement of the amount of nitric oxide (NO) produced

Under the same conditions as in the cell proliferation inhibition experiment, cells were treated with 62.5 μg/mL and 250 μg/mL of herb extract, and after 30 minutes, 1 μg/mL of LPS and 1 ng/mL of IFN-γ were treated, and a CO ₂ incubator for 72 hours. After culturing at, the amount of nitric oxide (NO) in the culture supernatant was measured using Griess reagent. 100 μL of culture medium and 100 μL of Griess reagent (1% sulfanilamide + 0.2% N-naphthylethyleme diamine 2HCl + 2.5% H ₃ PO ₄ ) were mixed and dispensed into a new 96-well plate, and the absorbance was measured at 570 nm within 10 minutes. A standard calibration curve was prepared using NaNO ₂ and the amount of NO produced by the sample was calculated. As shown in Table 1, the amount of nitric oxide that induces inflammation by promoting the secretion of inflammatory cytokines was 14.3% and 38.8% in a concentration-dependent manner in 62.5 μg/mL and 250 μg/mL of Daeja Lake extract. Showed.

NO inhibition rate (%) None 100 LPS+gIFN 0 Daejaho extract 62.5 (μg/mL) 14.3 ± 3.2 Daeja Lake extract 250 (μg/mL) 38.9 ± 2.9

6-3) Measurement of inflammatory mediators (TNF-α, PGE2)

After dispensing macrophages to the number of 10 ⁶ cells per well in a 24-well plate, 100 μg/mL of RAH13 drug and 100 nM of celecoxib as positive control were treated, and 1 μg/mL of LPS and 1 ng/mL of IFN-γ were treated after 30 minutes. After incubation in a CO ₂ incubator for 24 hours, centrifugation was performed at 2000 rpm for 5 minutes, and the supernatant was collected and used for cytokine measurement. The amount of TNF-α (BD Pharmigen, USA) and PGE2 (R&D systems, USA) in the collected supernatant was measured by enzyme immunoassay using a commercially available kit. As a result of the experiment, as shown in FIG. 5, TNF-α and cyclooxygenase-2 (COX-2), pro-inflammatory cytokines, which are inflammatory mediators that are closely related to the degree of inflammation As a result of measuring the production amount of PGE2, which is excessively produced in the inflammatory site by activity, causing inflammation and pain, the amount of PGE2 and TNF-α production decreased by about 10% and 17%, respectively, in 62.5 μg/mL of Daejaho extract. , The production of PGE2 and TNF-α in the treated group at 250 μg/mL of Daeja Lake extract was significantly reduced to about 35% and 77%.

6-4) Measurement of inflammatory cytokines (IL-1b, IL-6, IL-8) and chemokines (MCP-1)

Keratinocytes were treated with 1 μg/mL of LPS and 1 ng/mL of γIFN, incubated in a CO ₂ incubator for 24 hours, and then treated with 62.5 μg/mL and 250 μg/mL of Daeja Lake extract and cultured again for 48 hours. After incubation, the plate was centrifuged at 2000 rpm for 5 minutes to collect the culture supernatant. The amounts of IL-1b, IL-6, IL-8, and MCP-1 in the culture medium were measured by enzyme immunoassay using a commercially available kit (kits, BD Pharmigen, USA). As a result of the experiment, as shown in Fig. 6, the inflammatory cytokines IL-1b and IL-6 were inhibited by almost 100% even at a low concentration of 62.5 μg/mL, and IL-8 and MCP-1 expression were also 250 μg. In /mL, the reduction was excellent by 40.3% and 69%, respectively.

6-5) Anti-inflammatory and antioxidant efficacy signaling mechanism of Daeja Lake

Keratinocytes were treated with 1 μg/mL of LPS and 1 ng/mL of γIFN, incubated in a CO ₂ incubator for 24 hours, and then treated with 62.5 μg/mL and 250 μg/mL of Daeja Lake extract and incubated again for 18 hours. After incubation, proteins were collected from the cells, and after protein quantification, 20 μg of each were electrophoresed on 4-12% acrylamide gel, transferred to nitrocellulose paper, and ppERK, pp38, pJNK and IkBa antibodies were diluted 1:1000 for 24 hours. Reacted. After washing three times in PBST solution, a secondary antibody was attached and light was emitted using chemiluminecence. As a result of the experiment, as shown in FIG. 7, phosphorylation of pp38 and pJNK decreased in a concentration-dependent manner, and in the case of IkBa, it showed a tendency to decrease only at 62.5 μg/mL. In conclusion, the anti-inflammatory and antioxidant efficacy in inflammatory keratinocytes is thought to be due to the inhibition of signal transduction of pp38, pJNK, and IkBa.

As described above, the toxicity of Daeja Lake was not confirmed even when administered to the neck, and the stability of the raw material itself of Daeja Lake was confirmed by receiving suitable results from heavy metal and pesticide residue tests, and it inhibited cell proliferation in inflammatory keratinocytes, and It was confirmed that the amount of nitric oxide that promotes secretion and induces inflammation is also inhibited in a concentration-dependent manner of the white tiger extract. In addition, the levels of inflammatory mediators (TNF-α, PGE2), inflammatory cytokines IL-1b, IL-6, IL-8) and chemokine MCP-1) were also reduced to quantitatively evaluate the efficacy of anti-inflammatory and antioxidant mechanisms of white tiger extract. It could be verified by experimental values.

<Example 2: Manufacturing method of natural soap using Daeja Lake>

Steps 1 to 4 below are a process of preparing a mixed fermentation liquid containing a high-temperature aging Daeja Lake and herbal medicinal materials, and the process of the fifth step or higher is a process for producing natural soap using the same.

Step 1: High-temperature aging

In order to ensure that the natural shampoo of the present invention has the effect of preventing hair loss and promoting hair growth, herbal medicines used as Daejaho and herbal medicines are selected, washed with water, and aged at high temperatures.

The present invention selects a 2-5 weight ratio of lotus leaf, cypress, cypress, black bean, Eoseongcho, green tea, and machihyun based on 100 weight of Daeja Lake, rinses cleanly, and puts it in a maturation machine at 65-95℃ depending on the ingredients. It is aged at high temperature for 21 days. At this time, the set temperature of the aging period is more preferably 80 to 90°C, and the aging period is aged for 3 to 21 days differently depending on the material.

The Daeja Lake (mountain white mugwort, white mugwort) of the present invention is a herbaceous plant that is born in 1-2 years and has a richer scent than the wormwood, which is also used as a treatment for malaria. It is sometimes seen in the southern part of Korea and grows in the central or northeastern part of Korea. In China, it is mainly distributed in the northeastern Hwabuk, Gansuk, and Hapseo. Only leaves can be used in the first year, and stems and roots can be used in the second year. The green leaves and stems of Daeja Lake (White Tiger) contain a kind of sesquiterpene, sieversinin. Dried plants include alkaloids, pyrocatechol tannin, flavonoid, lactone, rutin, and isoquercetin, and sesquiterpene and y-lactone isolated from Daeja Lake (White Tiger) have inhibitory effects in vitro against Staphylococcus aureus and Escherichia coli.

Among the herbal medicines, lotus leaves have similar effects to lotus roots, but have the effect of quenching thirst, soothing the skin, improving energy, and detoxifying nicotine.

Cypress has excellent antibacterial, blood circulation, dandruff prevention, immunity strengthening, and natural healing effects. It contains phytoncide ingredients and has excellent sterilization effect.

Pyeonbaek (側柏) removes odor, darkens hair, strengthens teeth and bones, is effective in cirrhosis and liver cancer, and is effective in preventing hypertension and stroke.

Black beans normalize blood pressure, relieve inflammation, and vitamin E, unsaturated fatty acids, anthocyanins, and beta-carotene prevent aging, and estrogen, saponin, and glycinin expand blood vessels to relieve gray hair and hair loss symptoms.

Eoseongcho has antibacterial, immune-boosting, and anti-inflammatory properties. In particular, an ingredient called quercithrin has excellent blood-purifying action that clears blood by removing toxins from the skin and expanding capillaries.

Green tea contains a large amount of catechins that have strong anti-cancer effects, inhibits inflammation and bacterial infection, inhibits the rise of blood sugar, prevents aging with antioxidant activity, detoxifies heavy metals and nicotine, recovers from fatigue and removes hangovers, and reduces acidity. It has the effect of changing, removing cavities and bad breath, clearing the skin and preventing aging.

Machi-hyun is rich in dental cancer components such as lignin and molybdenum and acts to separate carcinogens, removes inflammation, removes itching, removes atopic inflammation and alleviates various dermatitis, and exhibits sebum control and anti-allergic effects. .

Step 2: making chotang

High-temperature-aged Daeja Lake and 15-25% by weight of herbal medicinal materials are put into a hot water heater with 75-85% by weight of purified water, and then high heat (the fire from the crater is in a state where the lever of the fire is fully opened, and the fire is enough to reach the bottom of the container. It means a firepower having a temperature of 98℃ or higher) and then boiled over low heat (the fire lever is almost closed, and the fire from the crater is barely alive and the surface of the material is lightly cut off). It refers to the thermal power having a temperature of 40~60℃ or higher) to make chotang after 48~72 hours.

Step 3: retangling manufacturing

Transfer the chotang made in the second step to another container, add 37-43% by weight of purified water, boil over high heat, and decoct over low heat for 3-4 hours to make re-tang.

Step 4: Preparation of mixed fermentation bath solution of chotang and re-tang

After removing the re-tanged Daeja Lake and the herbal medicinal material, the mixture of chotang and re-tang is immersed for 24 to 36 hours over low heat to obtain a mixed aging solution of 7.5 to 8.5% by weight of the total weight of the mixture.

Through the process of preparing the chotang and re-tang, and the mixing of cho-tang and re-tang to prepare a mixed fermented tang solution, firstly, the high-temperature aging herbal medicines are reduced and mutually compatible.

Step 5: Mixing the mixed aging liquid and caustic soda

After mixing 65-75% by weight of mixed aging liquid and 25-35% by weight of caustic soda, it is cooled to 40-55℃. It is mixed with caustic soda and reacted with only the mixed aging liquid without any additional water addition. Caustic soda reacts with the mixed aging liquid to release gas and generates high heat, which is cooled in air to reach 40~55℃.

Step 6: Saponification by adding carrier oil

The cooled caustic by heating 65-75% by weight of a mixed carrier oil including two or more of coconut oil, palm oil, olive oil, camellia oil, castor oil, rice bran oil, sunflower oil, and grape seed oil to 40-55℃ While slowly adding 25 to 35% by weight of a mixture of soda and crude drug aging liquid, the mixture is stirred and saponified.

When all of the carrier oils are added, the carrier oil can be weighed in a ratio of 1500 g of coconut oil, 500 g of palm oil, 800 g of olive oil, 400 g of camellia oil, and 300 g of castor oil.

Step 7: Add essential oil to the saponified mixture

To 95 to 98% by weight of the saponified mixture, 2 to 5% by weight of the mixed essential oil including two or more of lavender, lemon, cray sage, ylang-ylang, rosemary, tea tree, and cedarwood are added and stirred.

The reason for the addition of the essential oil is that most of the unpleasant odor of herbal medicines has been reduced through thermal aging, cho-tang, and re-tang, but the characteristic herbal odor remains, so it is added to improve this and add functional properties of each oil.

Among essential oils, lavender has a rich floral scent, has antiseptic effects, and is effective for acne, dermatitis, boils, eczema, insect bites, athlete's foot, ringworm, wounds, wrinkles, and helps treat stress, depression, insomnia, and headaches. do.

In addition, Lemon is a strong acidity that is effective in recovering from fatigue, blood circulation, activating metabolism, strengthening skin and mucous membranes, strengthening resistance to bacteria, and removing dead skin cells from oily skin, healing wounds, and cleaning hair. .

In addition, Clary sage has antiseptic, sterilization, nerve stabilization, odor prevention, and antipyretic effects on sensitive skin, stress relief and trauma treatment, seborrheic dandruff, and hair growth.

In addition, Ylang Ylang is effective in stabilizing effect, aphrodisiac effect, antiseptic effect, acne treatment effect, maintenance of normal blood pressure, pain relief effect, prevention of hair loss by stimulating the scalp, moisture balance control on the skin, and prevention of splitting of hair ends. have.

In addition, rosemary promotes cell regeneration and lymphatic discharge, and is effective for aging skin by promoting fibroacetogenesis, as well as promoting blood circulation, antibacterial, antiseptic, pain relief, inflammation relief, libido, and urination. It has a strong scent to energize brain cells, so it is effective for memory, concentration, prevention of dementia, maintaining shine and elasticity, preventing neuralgia, dandruff, hair loss, and hair growth.

In addition, tea tree is effective against skin diseases caused by bacteria, acne, eczema, oily skin, athlete's foot, warts, insect bites, dandruff, and wounds, and is effective in improving the immune system.

In addition, Cedarwood helps lymphatic drainage, decomposes cellulite, acne, oily hair, dandruff, antiseptic, promotes urination, astringent, and sterilizes, so it has the best effect on oily skin, healing acne, seborrheic scalp. Not only is it good for dandruff and alopecia, it is used for hair tonic because it has the effect of relieving nerves and promoting detoxification of scalp cells, and is effective in preventing hair loss.

Step 8: Thermal maturation to generate sufficient natural nutrients

The mixture to which the essential oil is added is poured into a mold, and in a completely sealed state, the mixture is heated and aged for 48 to 120 hours at a temperature of 30 to 40° C. to increase the maturity so that natural nutrients are generated.

The reason why this step is performed in a completely sealed state is that if the saponified mixture continues to contact air, it hardens in a short time. Since natural nutrients are not sufficiently produced while being hardened, sufficient natural nutrients and glycerin are produced before hardening, so that long-term thermal aging is performed in a sealed state.

Step 9: aging soap at room temperature

After the thermal aging, the solidified soap is separated from the mold and aged at room temperature at 22 to 28°C for 4 to 6 weeks in a shaded and ventilated place.

The tenth step (S1000): pH test to measure acidity

The product is completed by separating a piece of natural soap that has been aged at room temperature, performing a PH test to measure the acidity, and confirming that the acidity is about neutral to 9 (weak alkali).

Hereinafter, the characteristics of the natural shampoo containing a large-signal arc will be described in detail based on examples.

<Preparation of shampoo containing the great purple lake>

Shampoo containing Daeja Lake extract through the same process as in the Examples of the present invention (Example: Daeja Lake Chumulmul 1kg, Lotus Leaf 300g, Cypress 300g, Chongbaek 300g, Black Bean 300g, Eoseongcho 300g, Green Tea 300g, Mackin 300g, Purified Water 10.2kg) and shampoo (Comparative Example: lotus leaf 300g, cypress 300g, cypress 300g, black soybean 300g, Eoseongcho 300g, green tea 300g, machigo 300g, purified water 10.2kg) prepared without Daeja Lake extract as main ingredients) were prepared.

<Test method>

Test subject selection criteria: Men and women diagnosed with androgenetic alopecia aged 18 to 54, basic type diagnosed as M2 or higher or C2 or higher or U1 or higher, specific type diagnosed as V1 or higher or F1 or higher according to Basic and specific (BASP) classification. The subjects were male and female patients with androgenic alopecia, male patients diagnosed with more than 3 by the orwood-Hamilton classification, or female patients diagnosed with abnormalities by the Ludwig classification.

Equipment used: (1) Using a computer equipped with Folliscope® (Video microscopic camera and separate folliscope software Phototrichogram), you can analyze the main characteristics of hair, such as density, thickness, and growth rate. System equipment), (2) Digital camera Canon EOD 750D) (Used when taking a clinical picture, attached to a fixed cradle so that the subject's head and hairline can be photographed at the same angle and position).

Test procedure: Two weeks before the start of the test, the subject was determined, the consent was washed out, and a dot tattoo was performed on the test site. At the 8th, 16th, and 24th weeks of the test, 8 weeks of test samples were issued after the visual evaluation by Folliscope and photographing, stability evaluation, and questionnaire evaluation.

<Experiment result>

(1) Confirmation of change in average number of hairs (N/cm2)

The change in the number of hairs at week 24 was confirmed for the test group using the shampoo of the Example containing Daeja Lake extract and the control group using the shampoo of Comparative Example not containing Daeja Lake extract, and as a result of device evaluation through Phototrichogram, the number of hair (N/ cm2) are shown in Figs. 8a and 8b, the average number of hairs is shown in Table 2, and the number of hairs and the improvement rate are shown in Figs. 9a and 9b, respectively.

division 0 weeks 8th week Week 16 Week 24 Test group 78.65±12.95 80.30±13.98 85.96±14.02 90.83±14.67 Comparative example 82.65±25.76 80.57±19.30 80.48±18.90 81.52±19.50

As shown in FIGS. 8A, 8B, Table 2, and FIGS. 9A and 9B, in the case of the test group, statistically significant levels (p<005) such as 78.65 at 0 weeks, 80.30 at 8 weeks, 85.96 at 16 weeks, 90.83 at 24 weeks, etc. As a result, the number of hairs increased. On the other hand, in the case of the control group, statistically significant differences (p<005) such as 82.09 at 0 weeks, 80.57 at 8 weeks, 80.48 at 16 weeks, and 81.52 at 24 weeks could not be confirmed.

In addition, as a result of confirming the difference between the test group and the control group, it was confirmed that the number of hairs between the test group and the control group had a statistically significant difference (p<005). In the interaction analysis, it was also confirmed that the change pattern of the number of hairs in the test group and the control group for each week had a statistically significant difference (p<005).

As described above, it was confirmed that the shampoo containing the Daejaho extract has a hair growth promoting function.

(2) Stability evaluation

Subjective irritation sensation (itch, sticking sensation, burning, tingling, stiffness, and other abnormal sensations) and objective irritation reactions (erythema, swelling, papules, etc.) of the test subject according to the use of the Example Shampoo containing Daeja Lake extract. Dystrophy), the number of occurrences was confirmed and shown in Table 3.

Mild division Mild Moderate Severe Subjective stimulation itch 0 0 0 Feeling of sticking with a needle 0 0 0 Burning 0 0 0 Tingling 0 0 0 Stiffness 0 0 0 Other abnormal senses 0 0 0 Objective stimulus response Erythema 0 0 0 edema 0 0 0 papule 0 0 0 Other abnormalities 0 0 0

As shown in Table 2, the shampoo containing the Daejaho extract did not show any subjective irritation and objective irritation. Accordingly, the result of application to the human body is also considered to be harmless because the shampoo containing Daejaho was not found to have any other abnormal symptoms such as itching or burning.

As such, the shampoo containing Daeja Lake extract showed statistically significant anti-hair loss effect compared to the control sample. In addition, no specific adverse reactions were observed in all 46 subjects during the 24-week clinical trial period. In conclusion, the test sample containing all large characters is considered to be an effective and safe sample for preventing hair loss.

As described above, although the present invention has been described by limited embodiments and drawings, the present invention is not limited thereto, and the technical idea and the following by those of ordinary skill in the art to which the present invention pertains. It goes without saying that various modifications and variations are possible within the equivalent range of the claims to be described in.

Claims (4)

Natural shampoo using Daeja Lake having a hair loss prevention and hair growth promotion function, characterized in that it contains Daeja Lake extract as an active ingredient. The natural shampoo of claim 1, wherein the Daejaho extract is used with lotus leaf, cypress, cypress, black bean, Eoseongcho, green tea, and machihyeon. The method according to claim 2, based on 100 weight of the extract of Daeja Lake, lotus leaf, cypress, cypress, black bean, Eoseongcho, green tea, and machihyun are mixed in a ratio of 2 to 3 weights, respectively. Natural shampoo. Select 2 to 3 weight ratios of lotus leaf, cypress, cypress, black bean, Eoseongcho, green tea, and machihyun based on 100 weight of Daeja Lake extract, rinse thoroughly, and put in a maturation period at 65 to 95°C for 3 to 21 days depending on the ingredients. High temperature aging;
Add 15 to 25% by weight of high-temperature aged Daeja Lake and herbal medicinal materials with 75 to 85% by weight of purified water in a steamer, boil over high heat (98℃ or higher), and decoct over low heat (40 to 60℃) for 48 to 72 hours. Making steps;
Transferring chotang to another container, adding 37 to 43 weight ratio of purified water based on 100 weight of chotang, boiling over high heat, and simmering it over low heat for 3-4 hours to make re-tang;
Removing the re-tanged Daeja Lake and herbal medicines, and then decoating the mixture of chotang and re-tang for 24 to 36 hours over low heat to obtain a mixed aging solution of 7.5-8.5% by weight of the total weight of the mixture;
Mixing while slowly adding 45 to 55% by weight of caustic potassium to 45 to 55% by weight of purified water and cooling to 75 to 85°C;
63-73% by weight of the mixed carrier oil including two or more of coconut oil, olive oil, camellia oil, and castor oil is heated to 75-85°C and the cooled caustic potassium and purified water mixture 27-37% by weight is slowly added. While saponifying the mixture by stirring;
Mixing 10 to 20% by weight of sugar and 80 to 90% by weight of purified water and heating to 80 to 90°C while stirring;
Mixing and stirring the saponified mixture in 70-80% by weight and 20-30% by weight of the sugar water;
Placing the saponified mixture and the sugar water mixture in an airtight container and aging at room temperature at a temperature of 22 to 28°C for 1 to 3 weeks in a shaded and ventilated place to obtain a natural shampoo paste;
Mixing 47 to 57% by weight of the natural shampoo paste aged at room temperature and 43 to 53% by weight of the mixed fermentation solution, heating at a temperature of 85 to 95°C, and stirring for 12 to 36 hours;
Rice water 90.5 to 93.5 wt%, sugar 1 to 1.5 wt%, salt 1 to 1.5 wt%, molasses 1 to 1.5 wt%, EM stock solution 1 to 1.5 wt%, EM active solution 1 to 1.5 wt%, and rosemary 1.5 to 2 wt% % Is well mixed and sealed, and then 12.5 to 15% by weight of the EM fermentation solution obtained by fermentation at a temperature of 30 to 35 ℃ for 5 to 10 days is added to 85 to 87.5% by weight of the mixture of the well-mixed fermentation solution and natural shampoo paste And agitating;
Adding 0.1 to 5% by weight of salt to 95 to 99.9% by weight of a mixture of the natural shampoo paste to which the EM fermentation solution has been added, and stirring at a temperature of 80 to 95°C;
Adding 1 to 2% by weight of functional substances vitamin E and silk amino acid to 98 to 99% by weight of the mixture of the salt-added natural shampoo paste and the mixed fermentation solution;
Add 1 to 6% by weight of essential oil mixed with two or more of lavender, lemon, ylang-ylang, rosemary, and cedarwood to 94-99% by weight of a mixture of natural shampoo paste and mixed fermentation solution to which the functional substance is added, and stir And;
To obtain a natural shampoo by putting the mixture of the natural shampoo paste added with the essential oil and the mixed fermentation liquid in an airtight container and aging at room temperature at 22 to 28°C for 5 to 10 days in a shaded and ventilated place; A method of manufacturing a natural shampoo using Daeja Lake having a hair loss prevention and hair growth promotion function, characterized in that.

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