KR102074597B1 - Automatic injection device and method for immunity induction mass production of larva - Google Patents

Abstract

The present invention relates to a device and a method for automatically injecting an immunogen for immunity induced larva mass production, which is capable of rapidly and easily injecting a drug (an immunity induced material) through deactivation, injection, and an immunity induced liquid soak after spreading larvae on a loading unit. The present invention moves the fallen aggregated larvae to position the larvae in an injecting line groove on the loading unit without separate handwork. The method for automatically injecting an immunogen uniformly maintains an injecting amount of the immunity inducing liquid for each larva since the number of needles is similar for each larva. The present invention deactivates the larvae with cool wind without water (a drying step is not necessary and the larvae are deactivated faster than with water), thereby shortening working time. The present invention soaks the larvae in the immunity induced liquid for a longer time by separating a stabbing step by needles from an immunity induced liquid injecting step through immersion, thereby maximally increasing the injecting amount of the immunity induced liquid.

Description

Automatic injection device and method for immunity induction mass production of larva}

The present invention relates to an apparatus and method for automating immunogen injection for the mass production of immune-induced larvae, and more particularly, after the larvae are evenly spread on the loading part, the drug (immune-inducing substance, etc.) is inactivated, injected and immunized with immersion liquid. The present invention relates to an immunogenic injection automation apparatus and method for mass production of immuno-induced larvae.

Veterinary antibiotics have been used for growth and treatment, and have played a leading role in today's massive intensive and corporate farming. Growth-promoting antibiotics are antibiotics supplemented to feed at low levels for the purpose of promoting livestock growth, inhibiting intestinal harmful bacteria, preventing diseases, and improving the breeding environment. In addition, therapeutic antibiotics are antibiotics administered at high levels under the prescription of a veterinarian for the purpose of treating diseases in the event of livestock symptoms and diseases.

Recently, however, problems such as the emergence of resistant bacteria due to misuse of antibiotics in livestock feed, antibiotics in livestock products, weakened disease resistance of livestock, and ecosystem pollution due to antibiotic residues leaked into manure are emerging.

Therefore, it is urgent to prepare measures to prevent the abuse of antibiotics, consumer hygiene and safety of livestock products, strengthen the regulations on the use of antibiotics to improve domestic livestock competitiveness, and promote the non-antibiotic livestock certification policy.

Recently, development of natural antibiotics with new mechanisms of action to combat resistant strains caused by abuse of antibiotics has been sought. Insects such as silkworms have a strong antibacterial substance for self-defense through a long evolutionary history in poor environment, it is known to be very excellent as a natural antibiotic development material that can replace chemical antibiotics.

According to the prior art Korean Patent Publication No. 2008-0083111 (transgenic silkworm producing an antibody and a method for producing the same), a method of producing a recombinant antibody from the silk gland of the transgenic silkworm is disclosed. However, the Korean patent has been insufficient to develop a device for inducing insects with natural antibiotic peptides in large quantities.

In order to improve this, Korean Patent No. 10-1624224 discloses an automatic larvae injection device including a larval support, a larva moving part, an injection solution injection part, and a larval separation part. However, the injection device of the Korean registered patent has to be fixed to each of the larvae inserted into the larval support, and also to inject the injection liquid (drug) through the needle after penetrating the needle to each larvae, this process is It requires a lot of labor and requires a considerable time to inject drugs into dozens or hundreds of larvae. In addition, the injection device of the Korean registered patent has a problem that requires a somewhat complicated structure of the injection device to deliver a predetermined amount of drug into the injection needle.

Applicant Patent No. 10-1874180 discloses a larval mass production auto-injection device capable of simultaneously injecting a drug into a plurality of larvae. In the registered patent, the caterpillar is stunned by putting it in a water or ice bath, and in this case, it takes a long time to deactivate and recover, and moisture remains in the caterpillar (without drying). If you immerse in the drug after stabbing with the

In addition, the registered patent is to drop the larvae are placed on the loading portion, when the dropped larvae are stacked in a multi-layer or more than three layers, when the number of needles penetrating or stabbing the top larvae increases the larvae Has a high probability of dying, and if the number of needles per needle is different, there was a problem that drug injection is not uniform for each larvae. In order to solve such a problem, the device of the registered patent required manual work to unfold the fallen larvae one by one.

The present invention provides a caterpillar mass immunity induction apparatus and method in which the number of needles is almost uniform for each caterpillar by spreading the fallen larvae evenly on a load portion.

The present invention provides a method and apparatus for quickly deactivating a larvae without using water.

One aspect of the present invention

Spreading the larvae in a single layer on the loading section 10;

Growing the larvae by moving the load to the cooling water, the freezing tunnel 30 or the freezer;

Lowering the scanning portion 40 having a plurality of needles to form a wound or gap in the larvae on the loading portion;

It relates to a method for automating the larval immunogen injection comprising the step of immersing the loading portion or the larvae of the loading portion in the immunoduction solution.

In another aspect, the present invention

A swinging part 60 which shakes the loading part 10 and spreads the introduced larvae into a single layer;

A transfer unit 20 for moving the loading unit;

A refrigeration tunnel (30) for supplying cold air in the range of -10 ° C to -60 ° C to the loading unit to inactivate the larvae;

An injection part 40 having a plurality of needles, the lowering part of the loading part to form a wound or a gap in the larvae on the loading part; And

The larvae are immersed in the immuno-induced fluid, and relates to a larval immunogen injection automation device including an immune induction unit 50 through which the immuno-induced fluid is injected into the larvae through a wound or gap.

The larval immunogenic injection automation apparatus and method of the present invention can shake the dropped and agglomerated larvae, so that the larvae can be positioned in the groove of the injection line on the loading section without any manual work. Therefore, in the method for automating the larval immunogen injection of the present invention, since the number of needles is almost similar for each larvae, the amount of immunization solution injection can be maintained almost uniform for each larvae.

The larval immunogen injection automation device and method of the present invention can deactivate the larvae by cold air without using water (no drying process is required, and the larvae are deactivated faster than with water), thereby shortening the working time.

The caterpillar immunogen injection automation device and method of the present invention can separate the stinging step by the needle and immunization injection step through immersion, so that the larvae can be immersed in the immune inducing solution for a longer time, thereby maximizing the amount of immunoduction injection. It can increase.

1 is a schematic diagram of a larval immunogen injection automation device usable in the present invention.
Figure 2 shows an example of the loading portion and the swinging portion.
3 shows an example of a lattice separator, a loading part and a swinging part.
4 shows the scanning unit and the loading unit.
5 shows that the injection portion descends to insert the needle into the larva.
6 is a photograph in which experimental treatment tools of Examples and Comparative Examples are set.
7 is a photograph of the control and treatment (Examples and Comparative Examples) after recovery.
Figure 8 shows the larva insertion rate and the wound rate according to the size (width and depth) of the loading section scan line groove.
Figure 9 shows the larva insertion rate and the wound rate with or without the grid separator.

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings. However, the scope of the present invention is not limited to the description or the drawings for the following embodiments. In other words, the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In addition, the terms "comprises" or "having" described herein are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, and one or the same. It is to be understood that the present invention does not exclude in advance the possibility of the presence or the addition of other features, numbers, steps, operations, components, parts, or a combination thereof.

In addition, the terms "unit", "group", "module", and the like described in the specification mean a unit that processes at least one function or operation, which may be implemented by hardware or software or a combination of hardware and software.

Figure 1 is a schematic diagram of the larval immunogenic injection automation device usable in the present invention, Figure 2 shows an example of the loading and swinging portion, Figure 3 shows an example of the lattice separation, loading and swinging portion 4 shows the injection part and the loading part, and FIG. 5 shows the injection part descending to insert the needle into the larva.

The method for automating the larval immunogen injection of the present invention includes the steps of locating the larva evenly on the load, inactivation step, needle injection step and soaking step.

The method for automating the larval immunogen injection of the present invention may use the larval mass induction apparatus of FIGS. 1 to 5, but is not necessarily limited thereto.

1 to 5, the larval immunogen injection automation apparatus includes a loading unit 10, a transfer unit 20, a freezing tunnel 30, an injection unit 40, an immune induction unit 50, and a swinging unit 60. Include.

The larvae may be insect larvae (wheatworms, dongae, silkworms, slugs, etc.), but there is no particular limitation on this. For example, it may be a silkworm larva that may be utilized as a feed additive.

Positioning the larvae evenly on the loading section is a step of spreading the larvae stuck on the loading section.

Larvae have sticky limbs that tend to stick to the load surface or adjacent larvae and do not fall off.

According to the present invention, the larvae can be scattered by spreading the loading part left and right or front and rear.

The swing of the load can be carried out in various ways. For example, an operator can rock the loading section left or right or back and forth by hand. Also, as shown in FIG. 2, the loading unit swing may be made by various mechanical methods. Referring to FIG. 2, the oscillation part 60 may include a driving motor (not shown), a rotating plate 61, a connector 62, a connection part 63, a support plate 64, and a container 65.

The swinging part 60 may linearly reciprocate the loading part 10 through the rotating plate 61 and the connector 62 connected to the driving motor 61.

The loading part 10 reciprocates along the sliding bar 641 formed on the lower portion of the support plate 64. The larva that leaves the loading part by shaking may be recovered from the container 65 formed under the support base.

As shown in FIG. 2, the loading part 10 may have a bottom in a plate shape, and the scan line groove 11 and the protruding wall 12 may be formed on the plate at predetermined intervals. The scanning line groove may have a width and a depth enough to allow the larvae to be inserted. For example, the width of the scan line groove may be 3 to 20 mm, and the depth may be 3 to 20 mm.

A lubricating film 12 may be coated on the upper surface of the loading part (the upper surface between the scan line groove and the groove). The lubricating film may degrade the larvae's adhesive adhesion and more easily spread the larvae on the load. The lubricating film may be a known lubricant. For example, lubricants include silicone waxes, polyolefin waxes, teflon coatings, molybdenum coatings, and the like.

On the other hand, as shown in Figure 3, the present invention is located on the loading portion, the lattice separation unit 70 and a plurality of slit 71 through which the larvae pass through the lattice separation unit of the scanning line groove 11 The larvae may be positioned in the scan line grooves, including a swinging part 60 that swings along the length direction.

The lattice separator may be used without limitation as long as the structure has a slit of a size that the larvae can pass through. For example, the size of the slit may be 35 ~ 40mm (a) × 5 ~ 10mm (b). The lattice separator may reciprocate along a scan line groove 11 of a portion of the slit.

The lattice separator may be formed by fixing a plurality of rods (rods 72) horizontally and vertically. The diameter of the rod may be 5-10 mm.

In the method of automating larval immunogen injection, the lattice separation unit 70 is placed on the loading unit 10, the larvae are dropped onto the lattice separation unit, and the lattice separation unit is along the longitudinal direction of the scanning line groove 11. Oscillating to position the larvae in the scanning line groove.

The deactivation step is to inactivate the larvae by moving the load to a cooling water storage tank, an ice reservoir, a freezing tunnel 30 or a freezer.

Or the deactivation step is the deactivation step is 2 minutes to 7 minutes at -1 ℃ ~ -80 ℃, preferably 2 minutes 30 seconds to 6 minutes at -10 ℃ ~ -70 ℃, more preferably- It is a step for 3 to 5 minutes at 10 ℃ ~ -60 ℃.

The present invention can be groooy the larvae put the load in a known freezer (not shown).

On the other hand, the apparatus of the present invention may include a conveying part 20 for moving the loading part.

Transfer means known as the transfer unit 20 can be used. For example, the transfer unit 20 may be a sliding guide in the form of a conveyor belt or rail.

Referring to FIG. 1, the transfer unit 20 may include a conveyor belt 21, a support 22 disposed on the belt, and a driving unit (not shown).

The support part 22 may be detachably attached to the conveyor belt, and the loading part may be inserted and fixed therein. The loading part may be mounted on the support part to be transferred to the lower end of the scanning part through the freezing tunnel.

Referring to FIG. 1, the freezing tunnel 30 may include a freezer 31 for making cold air, a tunnel part 32 through which the loading part passes, and a pipe 33 for supplying cold air into the tunnel part. have. The refrigerator 31 may supply air at room temperature to the blow 34 to be converted into cold air and be supplied to the tunnel unit.

The refrigeration tunnel can be maintained at -1 ° C ~ -80 ° C, preferably -10 ° C ~ -70 ° C, more preferably -10 ° C ~ -60 ° C. Cold air in the range of -1 ° C to -80 ° C may be supplied to the loading portion to quickly deactivate the larvae and proceed with deactivation without moisture, and thus no separate drying process is required.

In the present invention, the larvae can be inactivated within 7 minutes, and the inactive time can be maintained for 20 seconds to 5 minutes, preferably 20 seconds to 3 minutes. It is advantageous for immune induction that the needle injection step is performed while the larvae are inactivated.

The needle injection step is a step of forming a wound or a gap in the larva on the loading portion by lowering the injection portion 40 having a plurality of needles.

1, 4 and 5, the scanning portion 40 of the present invention is a plate-shaped fixing plate 41, the end of the needle is fixed, a plurality of needles formed with a needle tip (tip) downward on the fixing plate (42) and a drive part (43) for vertically moving the fixed plate (41).

The scan part may include a pressing plate 44 and a pressing elastic part 45.

The pressing plate 44 may include a plurality of holes 441 which are spaced apart from the fixing plate 41 by a predetermined interval and penetrate through the needles.

One side of the pressing elastic part 45 is fixed to the fixing plate 41, and is coupled through the pressing plate 44. The pressing elastic portion 45 is provided with an elastic means therein. As the fixing plate and the pressing plate are lowered by the driving unit 43, the lower end of the pressing elastic part is first placed on the loading part. If the driving of the drive unit 43 continues, the fixed plate 41 and the needle 42 continue to descend and the pressing elastic unit is compressed. When a needle penetrates the hole 441 and pierces the larva to a predetermined depth, the driving part 43 raises the fixing plate. The fixing plate 41 and the needle 42 are raised first, and since the pressing plate is still holding the larva, the needle may be separated from the larva and raised. The pressing plate is then raised to return to its original position.

When the fixing plate is lowered to the loading part, the needle is arranged on the fixing plate 41 so that the needle 42 is located in the scanning line groove 11 of the loading part. That is, the needles may be arranged on the fixing plate at predetermined intervals so as to correspond to the scanning line grooves of the loading portion.

In the needle injection step, 1-4 needles per larval individual may form a wound or gap. If more than five needles are injected, the larvae may die.

The immersion step is a step of immersing the loading portion or the larvae of the loading portion in the immuno-induction liquid.

The immune inducing liquid may be a substance having an effect of regulating immune ability without being toxic to a living body. For example, the immune inducing liquid may be a biological response modifier (BRM), and specifically, may be AHCC (Active Hexose Correlated Compound), Polysaccarides, Polysaccaride peptides, Nucleosides, Triterpenoids, etc. Various substances can be applied to the present invention as immune inducing fluids.

The immunoduction solution immersion step may be 10 minutes or more. For example, the immuno-induction liquid immersion step may be 10 minutes to 2 hours.

Referring to FIG. 1, the immune induction part 50 may be a storage tank in which a predetermined amount of an immunoinduction solution is carried. When the larvae are immersed in the immuno-induced part, the immuno-induced liquid may be injected into the larvae through a wound or gap formed by the needle.

As described above, the method for automating the larval immunogen injection of the present invention can immerse the larvae in the immunization solution for a longer time, thereby maximizing the immunity injection amount.

The method or apparatus of the present invention may further include a control unit for controlling the operation of the oscillator, the transfer unit, the freezing tunnel, the injection unit and the immune induction unit.

The caterpillar immunogen injection automation device of the present invention may further include a sensor for detecting the operation of the oscillation unit, the transfer unit, the freezing tunnel, the injection unit and the immuno-induction unit.

The controller may control whether each device is operated, an operating time, a swing frequency and speed, a temperature condition, a conveying speed of the conveying unit, whether the scanning unit is raised and lowered, the falling and rising time, and the like.

The control unit may be a computer built-in and systemized by the user's input conditions or operating process (algorithm) in the form of a program command to execute the operation of the larval mass immunity inducing device.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited only to these examples.

Example 1

The stun time and recovery time of silkworms were measured under the same conditions as in Table 1 below.

Condition 18 ℃ water immersion
(Comparative Example 1) 4 ℃ immersion
(Comparative Example 2) 2 ℃
Water immersion
(Comparative Example 3) 4 ℃
Cold wind
(Comparative Example 4) -18 ℃ cold wind
Example 1 -55 ℃ cold wind
Example 2 Treatment ① ② ③ ④ ⑤ ⑥

 Twenty animals were randomly selected for each treatment (pattern silkworm, 5 days and 3 days), and compared to the control after measuring the stun time and recovery time after treatment according to the conditions. The recovery time and complete recovery time (including the recovery time in the recovery time) were measured.

Figure 6 is a photograph set the experimental treatment, Figure 7 is a photograph of the control, treatment after recovery. Table 2 below shows the stun and recovery time after treatment.

Condition 18 ℃ water immersion
(Comparative Example 1) 4 ℃ immersion
(Comparative Example 2) 2 ℃
Water immersion
(Comparative Example 3) 4 ℃
Cold wind
(Comparative Example 4) -18 ℃ cold wind
Example 1 -55 ℃ cold wind
Example 2 Treatment ① ② ③ ④ ⑤ ⑥ faint
time 7 minute, 30 seconds 7 minute, 30 seconds 7 minute, 30 seconds - 4 minutes 3 minutes 30 seconds tremor
time 1 minute 2 minutes 3 minute, 20 seconds - 20 seconds 3 minutes recovery
time 2 minutes 30 seconds 7 minutes 6 minute, 30 seconds - 30 seconds 5 minutes

Referring to Table 2, the time taken for stun (deactivation) of Comparative Examples 1 to 4 was 7 minutes and 30 seconds, but Examples 1 and 2 were shortened by 4 times and 33 minutes and 30 seconds, respectively. In addition, Examples 1 and 2 are shorter in recovery time, including fine time, than Comparative Examples 1 to 3. FIG.

As such, the present invention can reduce the deactivation time by more than two times as compared to the existing method, and can shorten the process time by not burying the larvae (no drying process is required).

Example 2

The size (? And depth) of the loading section scan line grooves, the insertion rate or the wound rate according to Table 3 were tested as follows.

Groove (mm)
Condition depth width A 8 13 B 8 10 C 10 13 D 6 13

Materials and methods

Type and age of experimental silkworms: 5 years old and 3 days old silkworm

Experimental Method: Four loading line scan line grooves were prepared. The silkworm was put on, and the horizontal cam motion was performed for 40 seconds and spread with a brush. Falling silkworms other than the scanning line grooves were separated into thin plates, and the state of insertion of the silkworms was monitored in the scanning line groove insertion rate, double insertion rate, and wound rate 10 times, and is shown in Table 4 and FIG. 8.

Insertion state (%)
Condition Scan line groove insertion rate
(Includes double insertion rate) Double penetration rate Wound rate A 58.12 ± 18.6 25.33 ± 5.26 8.58 ± 2.49 B 85.59 ± 10.98 0 5.36 ± 1.77 C 89.37 ± 9.1 30.13 ± 4.1 35.65 ± 10.43 D 80.4 ± 13.22 23.84 ± 6.9 28.99 ± 7.4

Referring to Table 4 and Figure 8, the shallower the depth of the scan line grooves, the deeper the double insertion rate, the higher the wound rate. In addition, when the width of the scanning line groove is wide, the double insertion rate is increased. The deepest scanning line groove depth and wide C condition showed the highest insertion rate. However, considering the double insertion rate, the B condition is the best condition because there is no double insertion and the wound rate is small.

Example 3

Conditional Test for Insertion of Silkworms in the Scanning Line of the Loading Section Using the Cam Separation

  Type and age of experimental silkworm: Patterned silkworm 5 years 3 days

     In this experiment, the most suitable loading section scanning line groove B condition (groove depth 8mm width 10mm), 10mm spacing was used.

   In condition a (FIG. 2), the loading part was cam-moved for 40 seconds in the horizontal direction, spread with a brush, and separated by pushing a thin plate of the dropping silkworm other than the scanning line groove.

   B condition (FIG. 3) was carried out for 40 seconds in the longitudinal direction by fixing the lattice (bag diameter 7m) at the loading site with the bottom rod horizontally and the upper rod vertically spaced (bottom 38mm, upper rod 7mm).

   The silkworm insertion state of two conditions was monitored 10 times by insertion rate and wound rate, which are shown in Table 5 and FIG. 9.

Insertion state (%)
Condition Scan Line Groove Insertion Rate
(Includes double insertion rate) Wound rate  a 73.2 ± 5.39 20.13 ± 8.36  b 93.1 ± 4.4 5.3 ± 3.97

Referring to Table 5 and FIG. 9, the b condition has a lattice separation part, and thus the scan line groove insertion rate is higher and the wound rate is lower than that of the a condition.

Those skilled in the art will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. Therefore, the scope of the present invention should not be limited to the above-described examples, but should be construed to include various embodiments within the scope of the claims and equivalents thereof.

Claims (8)

Spreading the larvae in a single layer on the loading section 10;
Growing the larvae by moving the load to a cooling water, a freezing tunnel 30 or a freezer;
Lowering the scanning portion 40 having a plurality of needles to form a wound or gap in the larvae on the loading portion;
Automated larval immunogenic injection method comprising the step of immersing the larvae of the loading portion or the loading portion in the immuno-induced liquid. The lattice according to claim 1, wherein the loading part (10) has scanning line grooves (11) at predetermined intervals, and the immuno-induction method includes a plurality of slits (71) on which the larvae can pass. Positioning the separating part 70, dropping the larvae into the lattice separating part, and swinging the lattice separating part along the longitudinal direction of the scanning line groove 11 to position the larvae in the scanning line groove. Larval immunogen injection automation method, characterized in that. The larvae of claim 1, wherein the loading part 10 includes the injection line grooves 11 at predetermined intervals, and the immuno-induction method includes swinging the loading part to position the larvae in the injection line grooves. Automated Immunogen Injection Method. The method of claim 1, wherein the deactivation step comprises rapidly cooling the larvae at a temperature of −1 ° C. to −80 ° C. for 2 minutes to 7 minutes. The method of claim 1, wherein the immunization solution dipping step is at least 10 minutes. A loading part 10 having a plurality of scanning line grooves 11;
A transfer part 20 for moving the loading part;
A refrigeration tunnel (30) for supplying cold air of less than -1 ° C to the loading unit to inactivate larvae;
An injection unit (40) having a plurality of needles and descending to the loading unit to form wounds or gaps in the larvae on the loading unit; And
The caterpillar immunogen injection automation device comprising an immune induction unit 50, the larva is immersed in the immuno-induced fluid is injected into the larva through the wound or gap. The method of claim 6, wherein the immuno-induction device is located on the loading portion, the lattice separation unit 70 having a plurality of slits 71 through which the larvae pass and the lattice separation unit the scan line groove 11 The larval immunogen injection automation device, characterized in that further comprising a rocking portion (60) to swing along the longitudinal direction of the larvae in the injection line groove. According to claim 6, wherein the freezing tunnel 30 includes a freezer (31) for making cold air, a tunnel portion 32 through which the loading portion passes, and a pipe 33 for supplying cold air into the tunnel portion. Caterpillar immunogen injection automation device characterized in.

Images