KR101768626B1 - Skin whitening composition comprising an extract obtained from carthamus tinctorius l. - Google Patents

Abstract

The present invention relates to a whitening cosmetic composition comprising a safflower extract, a whitening food composition, a health functional food for preventing or alleviating a pigmentation disease comprising the composition, and a pharmaceutical composition for preventing or treating pigmentation disorders will be.
The composition containing the safflower extract of the present invention has the advantages of low skin irritation and low cytotoxicity, but also inhibits the production of melanin and inhibits tyrosinase activity. Therefore, a functional cosmetic for skin whitening, skin pigmentation And can be usefully used as a health-functional food or a skin pigmentation disease therapeutic agent capable of preventing or alleviating a disease.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin whitening composition comprising a safflower extract,

The present invention relates to a whitening cosmetic composition, a whitening food composition, a health functional food for preventing or alleviating pigmentation diseases, and a pharmaceutical composition for preventing or treating pigmentation diseases.

The skin color of a person is determined by the amount of melanin, carotene and hemoglobin, among which melanin has the greatest effect and is also influenced by skin thickness, reflectance and blood flow.

The melanin pigment, which is the most important factor for determining skin color, is a type of amino acid normally present in the human body. The tyrosine is converted into waveguide by tyrosinase, an enzyme contained in melanocytes existing in the basal layer of the epidermis. Through a complicated oxidation process, melanin, which is a dark brown polymer, is finally produced. Here, genetic factors, environmental factors, hormones, foods, medicines and other chemicals act as factors of synthesis.

Tyrosinase is a metal-containing protein that is distributed in almost all species, including microorganisms, plants, and animals, and contains a pair of copper ions at the active site. Studies on the whitening agents so far have mainly focused on the development of the activity inhibitors of tyrosinase.

In general, the mechanism of action of tyrosinase activity inhibitors is classified into two types. First, it is chelated with copper ion contained in the active site of tyrosinase, and kojic acid is commercialized as a typical enzyme inhibitor. The second is related to the inhibition of tyrosinase activity and mechanism, but it is weak or not enough to chelate with copper ion. As a representative material, arbutin, the leader of whitening agents, is commercialized.

The function of tyrosinase is closely related to disease. In animals, abnormal melanin staining, such as vitiligo and pigmentation, is caused by abnormalities in the synthesis of melanin, which can cause skin cancer in severe cases. Therefore, it is very important to study melanin synthesis inhibitors for the development of novel medicines for treating melanin pigmentation abnormalities and functional cosmetics aimed at whitening.

Currently known methods for whitening include UV blocking, signal suppression, inhibition of gene expression, inhibition of enzyme action, pigment reduction, and stratum corneum removal. Arbutin, kojic acid, vitamin C, hydroquinone and the like are used as a representative single substance showing whitening function, and currently natural substances for the development of natural whitening agents are actively under investigation.

On the other hand, in general, with increasing environmental pollution, the preference for white skin is increased due to skin damage due to ultraviolet rays, and interest in a method for obtaining a skin whitening effect as the clear and clean skin is pursued with transparent make-up is heightened have. Human skin color is affected by environment, race, sex, etc., but is generally determined by the content of brown melanin.

Over-deposition of melanin pigment in the skin may result in hyperpigmentation, phototoxic response or other similar small-sized post-inflammatory reactions due to scarring or dermatitis, including unwanted freckles, senile spots, liver, spots, brown or black spots, It can cause fixed pigmented lesions.

The prior art related to this is as follows.

Korean Patent Registration No. 10-0841276 (registered on June 19, 2008, Korea Food Research Institute) discloses a skin whitening composition containing a skin white ginseng ethyl acetate fraction having excellent whitening activity. More specifically, A skin whitening composition containing a skin white ginseng ethyl acetate fraction.

Korean Patent Registration No. 10-0850686 (registered on July 31, 2008, patent holder Inha University Industry-Academy Collaboration Foundation), extracts of Nigella glandulifera Freyn et Sint having skin whitening activity and a whitening cosmetic composition Which specifically inhibits the production of melanin in melanin-producing cells, and a whitening cosmetic composition containing the same as an active ingredient.

Korean Registered No. 10-1121736 (registered on Feb. 22, 2012; patent rights holder Sajimang Cosmetics Co., Ltd.) discloses extracts of herbal medicines for skin whitening, methods for their production, and whitening cosmetic compositions containing them, , Red ginseng extract, extract obtained from blue deep-sea consonants, and cosmetic composition containing the same.

Korean Patent Application No. 10-2012-0053242 filed on May 18, 2012 (filed by Kolmar KK) discloses a plant extract having a skin whitening effect derived from a succulent plant and a cosmetic composition for whitening skin containing the same as an active ingredient .

Korean Patent Registration No. 10-0604085 filed on Jul. 18, 2006, KORIANA Cosmetics Co., Ltd.) is a whitening cosmetic composition comprising an upper body extract and glutathione, wherein the cosmetic composition comprises 0.1 to 20.0% by weight, preferably 1.0 To 10.0% by weight, and glutathione is contained in an amount of 0.001 to 5.0% by weight, preferably 0.01 to 1.0% by weight, based on the total weight of the composition.

Korean Patent Registration No. 10-0345226 (filed on Jul. 06, 2002, Amorepacific Group Co., Ltd.) is a cosmetic composition having excellent skin whitening effect, and more specifically, a whitening agent There is disclosed a cosmetic composition for whitening wherein astaxanthin extract is mixed with at least one extract selected from the group consisting of Aomocho seed extract, Rhizoma seed extract, Motherwomb extract, Rhizoma extract, and Licorice extract.

Korean Registration No. 10-0515206 (registered on September 08, 2005; patentee Kim Jong-seok et al.) Contains the extract of Baekjonggong as a main component and if necessary, ranunculin, Deoxypodophyllotoxin, 3-O- alpha -L-raminopyranosyl (1 → 2) - [β-D-glucopyranosyl (1 → 4)] - α-L-arabinopyranoside SB365), wherein a whitening cosmetic composition containing at least one auxiliary ingredient selected from the group consisting of dandruff and licorice extract is described.

The prior art including the above-described documents has not been taught about a composition for whitening using an extract of Safflower Sulfur.

As a result, the present inventors have found that the extract of Safflower Sulfur has a tyrosinase activity which is superior to arbutin, which is known as a whitening agent, within a range that does not cause toxicity to the cells, as a result of efforts to find a substance having excellent skin whitening effect Inhibitory effect and melanin production, the present invention has been completed.

An object of the present invention is to provide a whitening cosmetic composition and a food composition obtained from a natural substance, which contains a substance having an excellent skin whitening effect without adverse effects on the human body.

It is also an object of the present invention to provide a health functional food for preventing or alleviating a pigment deposition disease which contains a substance obtained from a natural substance and which has a superior skin whitening effect without causing adverse effects on the human body and a medicament for the prevention or treatment of a pigment deposition disease Gt;

In order to achieve the above object, the present invention provides a whitening cosmetic composition comprising an extract of safflower sulfur as an active ingredient.

Further, the present invention provides a whitening food composition comprising an extract of Safflower as an active ingredient.

Further, the present invention provides a health functional food for preventing or alleviating a pigment deposition disease containing an extract of safflower yellow as an active ingredient.

Finally, the present invention provides a pharmaceutical composition for the prevention or treatment of a pigmentation disorder containing an extract of safflower sulfur as an active ingredient.

The composition containing the safflower extract of the present invention can inhibit the production of melanin and inhibit tyrosinase activity while having no skin irritation and low cytotoxicity, so that it can be used as a functional cosmetic for skin whitening, a skin pigment Can be usefully used as a health functional food or a skin pigmentation disease treatment which can prevent or alleviate a deposition disorder.

1 is a graph showing the inhibitory effect of tyrosinase activity of the safflower extract of the present invention.
2 is a graph showing the inhibitory effect on intracellular melanin biosynthesis of the safflower extract of the present invention.

The present inventors have discovered for the first time a whitening use of Carthamus tinctorius L. extract. The inventors of the present invention found that a safflower extract inhibits excellent tyrosinase activity and inhibits melanin production, The whitening effect is excellent, thereby completing the present invention.

In the present invention, " Carthamus tinctorius L. ", also referred to as safflower, is an ovary with no ovary and 1 to 2 cm in length. The outer surface is reddish to reddish brown, the flower corolla is thin and long, the tip is divided into 5 pieces, and the ground piece is narrow line shape and 5 to 8 mm in length. Surgery is 5, and anther is gathered to form a tubular shape and is yellowish white. The stigma has a long cylindrical shape and the top is slightly forked. Quality is flexible. There is a peculiar smell and the taste is slightly bitter. Its pharmacological actions include uterine contraction, vasodilatation and hypotensive action, blood coagulation inhibition and anti-thrombotic action, antioxidative action, antiinflammatory action and the like. Due to this pharmacological action, blood disorders (gynecological diseases, ) Therapeutic materials, cosmetics coloring (lip balm), food coloring are used.

In the present invention, safflower sulfur may be purchased commercially, sold, or harvested or cultivated in nature.

The safflower sulfur extract of the present invention is preferably produced by a production method comprising extracting safflower sulfur or a pulverized product thereof with water, preferably distilled water, to obtain a safflower extract, and filtration or drying The process is not limited to this. Specifically, a conventional extraction method such as an ultrasonic extraction method, a filtration method, and a reflux extraction method can be used as a method for producing the safflower sulfur extract.

Preferably, the safflower dried product obtained by pulverizing the safflower with the foreign substance removed by washing and drying can be extracted with water, preferably distilled water. At this time, the amount of the extraction solvent is preferably 2 to 20 times the dry weight of the safflower sulfur.

Further, at the time of extraction of the safflower sulfur, it can be extracted at room temperature or preferably with heating, more preferably at 60 to 100 ° C, and the extraction can be carried out at this temperature for about 2 to 4 hours. When the extraction temperature and the extraction time are out of the above range, the extraction yield is lowered, and when the extraction temperature is lowered, the extraction time is excessively long.

In this case, after the extraction, separation and purification, for example, concentration or freeze-drying, may be additionally performed.

The examples of the present invention show that the safflower extract of the present invention significantly reduced the production of? -MSH-induced melanin within a range that does not cause toxicity to the cells, and inhibited the expression and activity of tyrosinase Respectively. In particular, it was confirmed that the safflower extract of the present invention has the most excellent effect of significantly reducing α-MSH-induced melanin production and tyrosinase expression (see FIGS. 1 and 2). From these results, it was confirmed that the extract of Safflower had high whitening activity.

Accordingly, the safflower extract of the present invention may have a function of helping to reduce the color of the melanin pigment deposited on the skin, and prevent the melanin pigment from being over-deposited on the skin to suppress the occurrence of skin pigmentation disease, To help with whitening.

Accordingly, the present invention can provide the following whitening composition using the safflower extract of the present invention.

The term "whitening" in the present invention means a method of increasing the brightness of the skin whose brightness has been reduced due to excessive pigment such as melanin or keeping the brightness of the skin at a certain level, It can be inclusive. For the purpose of the present invention, the whitening is carried out by means of inhibiting the synthesis of melanin formed in the skin by the safflower sulfur extract, but is not particularly limited thereto.

As a reference, melanin is a pigment component widely distributed in living organisms such as skin, hair, eyes and the like. It is synthesized in melanosome in the melanocyte of the skin layer of the body. Tyrosinase (tyrosinase) melanin is biosynthesized by the oxidation and polymerization reaction of DOPA (3,4-dihydroxy-phenylalanine) or DOPA quinone with tyrosine as a starting material. Biosynthesized melanin increases resistance to skin irritation such as ultraviolet rays, but because excessive melanin synthesis causes pigmentation such as spots, freckles, and black spots, tyrosinase activity measurement is considered as an important indicator of skin whitening effect have.

One aspect of the present invention provides a whitening cosmetic composition comprising an extract of safflower sulfur as an active ingredient.

The whitening cosmetic composition of the present invention is characterized by comprising the above-described extract in an amount of 0.0001 to 50% by weight, based on the total weight of the cosmetic composition, and a cosmetic base as a remaining amount. The cosmetic base constituting the cosmetic composition may be a cosmetic base for constituting a soft longevity (skin lotion), a nutritional lotion (nutrition lotion), an essence, a cream, a body cream or a pack.

In this case, the content of the dry powdery extract in the cosmetic composition is 0.0001 to 5% by weight, the content of the liquid is 1.3 to 50% by weight in the case of 1.3-butylene glycol, propylene glycol, dipropylene glycol, glycerin and a mixture thereof, Preferably 0.01 to 1% by weight for the former, and 0.1 to 10% by weight for the latter. In addition, the composition refers to the addition of the extract to cosmetics, that is, softening longevity (skin), nutritional lotion (lotion), essence, nutrition cream, pack, body lotion, body oil and the like.

The whitening cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, -Containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto. More specifically, the whitening cosmetic composition of the present invention can be used as a cosmetic composition for softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder .

The whitening cosmetic formulation of the present invention may be used as a carrier component in the case of a paste, a cream or a gel in which an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, Zinc oxide and the like can be used. The whitening cosmetic formulation of the present invention can be used as a carrier component in the case of a powder or a spray, such as lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder. The whitening cosmetic formulation of the present invention may be a solution or emulsion, in which a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Glycolate, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used. The whitening cosmetic formulation of the present invention may contain, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester Suspension, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth can be used. In the whitening cosmetic formulation of the present invention, it is preferable that, in the case of a surfactant-containing cleansing, the cosmetic composition containing as the carrier component an aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

 The whitening cosmetic composition of the present invention may be used in combination with an acute or chronic moisturizing agent (for example, a wetting agent, a sealant, and an agent that affects the natural moisturizing mechanism of the skin), an antioxidant, a sunscreen agent having UVA and / Antioxidants, antioxidants, vitamins, trace metals, antimicrobial agents, vegetable extracts, fragrances, and / or other beneficial agents and compounds such as dyes and color components.

In addition, the cosmetic composition for whitening of the present invention may contain a surfactant. The surfactant may be a compatible mixture of an anionic surfactant, a cationic surfactant, a nonionic surfactant or a surfactant. The surfactant may also be an ampholytic or amphoteric surfactant having the characteristics of anions or cations depending on the pH of the composition.

In addition, the whitening cosmetic composition of the present invention may also contain a hydrotrope. Hydrotropes are compounds that have the ability to enhance water solubility of other compounds. Specific examples of hydrotropes include, but are not limited to, sodium cumene sulfonate, ammonium cumene sulfonate, ammonium xylene sulfonate, potassium toluene sulfonate, sodium toluene sulfonate, sodium xylene sulfonate, toluene sulfonic acid and xylenesulfonic acid. Other useful hydrotropes include sodium polynaphthalene sulfonate, sodium polystyrene sulfonate, sodium methyl naphthalene sulfonate, sodium camphor sulfonate, and disodium succinate.

In addition, the whitening cosmetic composition of the present invention may contain a thickening agent or a gelling agent. The thickener or gelling agent may be, for example, a polymer or copolymer unsaturated carboxylic acid or unsaturated ester, polysaccharide derivative, gum, colloidal silicate, polyethylene glycol (PEG) and derivatives thereof, polyvinylpyrrolidone and its derivatives, Derivatives, polyacrylonitrile, hydrophilic silica gel or mixtures thereof.

Another aspect of the present invention provides a whitening food composition comprising an extract of Safflower as an active ingredient.

The whitening food composition of the present invention may contain the safflower sulfur extract.

The whitening food composition may have a function of helping to reduce the color of the melanin pigment deposited on the skin and prevent excessive deposition of the melanin pigment on the skin to suppress the occurrence of skin pigmentation disease, You can have a helping function.

The whitening food composition of the present invention includes forms such as a pill, powder, granule, precipitant, tablet, capsule or liquid, and the food to which the composition of the present invention can be added includes, for example, various foods, For example, drinks, gum, tea, vitamin complex, and health supplement foods.

In addition to the safflower extract, other components that do not interfere with whitening activity may be added to the food composition of the present invention, and the kind thereof is not particularly limited. For example, various herbal medicine extracts, food-acceptable food-aid additives or natural carbohydrates, such as ordinary foods, may be added as an additional ingredient.

As used herein, the term "food-aid additive " refers to a component that can be added to foods in a supplementary manner, and is appropriately selected and used by those skilled in the art as added to produce health functional foods of each formulation. Examples of food aid additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, and carbonating agents used in carbonated beverages. However, the types of the food-aid additives of the present invention are not limited by these examples.

Examples of such natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above .

Another aspect of the present invention provides a health functional food for preventing or alleviating a pigmentation disease comprising the food composition of the above aspect.

The term " health functional food " used in the present invention refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials and components having useful functions in the human body. Here, the term " functional " means that the structure and function of the human body have a beneficial effect for health use such as controlling nutrients or physiological action. The health functional food of the present invention can be manufactured by a method commonly used in the art and can be prepared by adding raw materials and ingredients which are conventionally added in the art. Also, unlike general medicine, there is an advantage that there is no side effect that can occur when a medicine is used for a long time by using food as a raw material, and it is excellent in portability.

In addition, prevention or alleviation of skin pigmentation disease in the present invention means that the composition containing the safflower extract is applied to hyperpigmented skin, thereby not only preventing melanin pigment from being deposited on the skin, It can mean any act that mitigates the melanin deposits.

The skin pigmentation disorder is caused by excessive pigmentation after drug use such as freckles, senile spots, liver, stain, brown or black spot, daylight pigment, cyanic melasma, calcium antagonist, sclerotherapy Post-inflammatory hyperpigmentation, phototoxic response, or other similar small fixed pigmented lesions due to scarring or dermatitis due to scarring and scarring, including burns, scabies and burns in women who have taken oral contraceptives But is not limited thereto.

The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, the safflower sulfur extract of the present invention is added in an amount of 1 to 10% by weight, preferably 5 to 10% by weight, of the raw material composition when the food is prepared. However, in the case of long-term ingestion intended for health and hygiene purposes or for the purpose of controlling health, the above amount can also be used below the above-mentioned range.

There is no particular limitation on the kind of the food. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating a pigmentation disorder containing an extract of Safflower as an active ingredient.

The pharmaceutical composition of the present invention may contain the safflower sulfur extract.

Preventing or alleviating the skin pigmentation disease in the present invention means not only preventing the deposition of melanin pigment on the skin by applying a composition containing the safflower extract to the hyperpigmented skin, It can mean all the actions of treating melanin.

The skin pigmentation disorder may include hyperpigmentation after drug use such as freckles, senile spots, liver, spots, brown or black spots, daylight pigmentation, blue blotch, calcium antagonist, aftereffects due to tissue hardening therapy, But are not limited to, hyperpigmentation, post-inflammatory hyperpigmentation due to scarring or dermatitis including scabies and burns in women taking birth control pills, phototoxic response or other similar small fixed pigmented lesions.

The pharmaceutical composition of the present invention can be used as a single preparation, and can also be manufactured and used as a composite preparation, further comprising a pharmaceutical composition known to have a recognized whitening effect.

 The pharmaceutical composition of the present invention may be formulated into a pharmaceutical unit dosage form by adding a pharmaceutically acceptable carrier, excipient, or diluent.

 By "pharmaceutically acceptable" as used herein is meant not significantly irritating the organism and not interfering with the biological activity and properties of the administered active substance.

The compositions of the present invention comprising a pharmaceutically acceptable carrier may be of various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances and preservatives may be included have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The pharmaceutical composition may be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, It can have one formulation.

In addition, the safflower sulfur extract in the composition of the present invention may be contained in a pharmaceutically effective amount. The term "pharmaceutically effective amount " as used herein means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will vary depending on the species and severity, age, sex, , Sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts.

The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And can be administered singly or multiply. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art. Preferably, the safflower extract in the present invention may be contained in the composition in an amount of 0.001 to 200 μg / ml, more preferably 0.001 to 100 μg / ml.

The pharmaceutical composition comprising the safflower sulfur extract of the present invention can be administered to an individual to prevent or treat skin pigmentation diseases.

The subject is a person who is in need of prevention or treatment of skin pigmentation disease and is a person who needs treatment of skin pigmentation diseases and similar symptoms as well as human beings, horses, sheep, pigs, goats, camels, , ≪ / RTI > and the like.

The term "administering" in the present invention means introducing the composition of the present invention into a subject by any suitable method, and the administration route of the composition of the present invention may be administered through any conventional route so long as it can reach the target tissue have.

The number of times application of the composition of the present invention can be determined depending on the prescription, necessity or desire. The composition of the present invention may be prescribed in parallel with a surgical method such as a laser. It can also be incorporated into casts, bandages, dressings, gauze pads, and similar items.

The present invention includes administering a pharmaceutically effective amount of a safflower extract. It will be apparent to those skilled in the art that the appropriate total daily dose may be determined by the practitioner within the scope of sound medical judgment. In addition, it can be administered once or several times in divided doses. For purposes of the present invention, however, the specific therapeutically effective amount for a particular patient will depend upon the nature and extent of the reaction to be achieved, the particular composition, including whether or not other agents are used, the age, weight, Sex and diet of the patient, the time of administration, the route of administration and the rate of administration of the composition, the duration of the treatment, the drugs used or concurrently used with the specific composition, and similar factors well known in the medical arts.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

Example 1 Preparation of Safflower Sulfate Extract of the Present Invention

1 kg of safflower sulfur was weighed and ground into powder and extracted with distilled water. Five times as much distilled water as the sample was added and the mixture was heated at 80 ° C for 2 hours for extraction. The extracted sample was filtered, concentrated by a vacuum concentrator, and then lyophilized (FDU-2100, EYELA, Japan).

<Experimental Example 1> Effect of in vitro tyrosinase inhibitory activity on the whitening effect of safflower extract

Mushroom tyrosinase inhibition assay was used to confirm the inhibitory effect of the safflower extract on tyrosinase enzyme activity. 10 μl of the sample and 20 μl of the sample were added to 170 μl of a reaction mixture containing 1 mM L-tyrosine, 50 mM potassium phosphate buffer (pH 6.5), and distilled water at a ratio of 10: 10: 9 Mu] l of a solution of mushroom tyrosinase (1000 units / ml) was added and the reaction solution was incubated at 25 [deg.] C for 30 minutes. Thereafter, the amount of dopachrome generated at 490 nm was measured by absorbance using a microplate reader. As a positive control, kojic acid, which is known as inhibitor of tyrosinase enzyme activity, was used. The percentage formula of inhibition of tyrosinase enzyme activity is shown in Equation 1 below.

[Equation 1]

Tyrosinase activity inhibitory effect (%) = {1- (A-B) / C} 100

[Wherein A is the sample absorbance, B is the color control absorbance, and C is the control absorbance]

The inhibitory activity of tyrosinase enzyme activity of the safflower sulfur extract calculated by the above formula (1) is shown in Table 1 and FIG.

Sample IC ₅₀ ([mu] g / ml) Safflower extract 159.3 Arbutin 215.31 Kojic aicd 4.84

IC ₅₀ values were 159.3 ㎍ / mL for Safflower extract and showed higher enzyme activity than arbutin (215.31 ㎍ / mL) for positive control.

Experimental Example 2 Effect of Safflower Sulfate Extract on Melanin Synthesis in Melanoma Cells

B16F10, a mouse melanocyte cell, was treated with 200 nM of? -MSH to induce melanogenesis, and the activity of whitening by the safflower extract was analyzed.

Specifically, 1 x 10 &lt; ⁵ &gt; B16F10 cells were placed in a D100 cell culture dish and cultured for one day. After the sample treatment, the cultured cells were washed with 1 × phosphate buffered saline (PBS), treated with 1 N sodium hydroxide (NaOH) containing 10% dimethyl sulfoxide (DMSO) Lt; / RTI &gt;

Subsequently, the absorbance was measured at 475 nm using a microplate reader. A standard curve was drawn using the synthesized melanin solution, and the amount of melanin produced was calculated by substituting the measurement value. As a positive control, arbutin and kojic acid, which are well known as whitening active substances, were used, and the measured values were expressed as average values of three repeated experiments.

As a result, as shown in FIG. 2, the extract of Safflower significantly decreased the production of alpha-MSH-induced melanin and showed similar activity to arbutin, which is a positive control. When the activity of the safflower extract was compared with that of arbutin, the activity was similar to that of arbutin, which is a single substance, suggesting that the safflower extract has a very high whitening activity.

From the above results, the whitening activity of Safflower extract was analyzed by inhibition of melanin formation and inhibition of tyrosinase activity. As a result, it was confirmed that melanin formation inhibitory effect and tyrosinase activity inhibitory effect were effective. Among them, tyrosinase activity inhibitory activity was confirmed to be excellent.

These results suggest that the safflower sulfur extract of the present invention effectively inhibits tyrosinase expression and consequently inhibits melanin synthesis, thereby being able to treat not only a whitening effect but also a disease caused by melanin deposition.

&Lt; Preparation Example 1 > Preparation of whitening cosmetic product

<1-1> Production of softening longevity

Examples of the components of the softening agent of the present invention are as follows:

0.05% by weight of the extract of Example 1

0.5% by weight of polyoxyethylene hardened castor oil

Glycine 3.0 wt%

0.1% by weight of dipotassium glycylate

3.0% by weight of 1,3-butylene glycol

0.1% by weight sodium &lt; RTI ID = 0.0 &gt;

5.0% by weight of ethanol

Antioxidant 0.1 wt%

0.1% by weight triethanolamine

EDTA 0.1 wt%

Preservative, purified water

<1-2> Preparation of whitening essence

Examples of components of the whitening essence of the present invention are as follows:

0.2 wt% of the extract of Example 1

Glycerin 5.0 wt%

Butylene glycol 2.0 wt%

Polyethylene glycol 2.0 wt%

Carbomer 1.0 wt%

Sodium hyaluronate 0.1 wt%

Glycine 3.0 wt%

Polyacrylamide 2.0 wt%

0.2% by weight of hydroxyethylcellulose

3.0% by weight of ethanol

Polyoxyethylene hydrogenated castor oil 1.0 wt%

Antioxidant 0.3 wt%

0.1% by weight triethanolamine

EDTA 0.1 wt%

Preservative, purified water

<1-3> Preparation of whitening cream

Examples of ingredients of the whitening cream of the present invention are:

0.5% by weight of the extract of Example 1

3.0% by weight of 1,3-butylene glycol

Glycerin 3.0 wt%

Hydrogenated lecithin 1.0 wt%

3.0 wt% octyldodecanol,

Trioctanone 2.0 wt%

Stearic acid 1.5 wt%

Cetostearyl alcohol 2.0 wt%

Polysorbate 60 1.5 wt%

Sorbitan sesquioleate 2.0 wt%

Dimethicone 3.0 wt%

Antioxidant 0.3 wt%

0.2% by weight of xanthan gum

0.1% by weight triethanolamine

EDTA 0.1 wt%

Preservative, purified water

<1-4> Manufacture of whitening pack

Examples of components of the whitening pack of the present invention are as follows:

0.2 wt% of the extract of Example 1

Glycerin 7.0 wt%

3.0% by weight of 1,3-butylene glycol

Squalene 3.0 wt%

Dimethicone 3.0 wt%

Sodium hyaluronate 0.1 wt%

Glycine 2.0 wt%

5.0% by weight of polyacrylamide

Antioxidant 0.3 wt%

0.1% by weight triethanolamine

EDTA 0.1 wt%

Preservative, purified water

Preparation Example 2 Preparation of whitening health functional food

<2-1> Production of flour food

0.5 to 5.0 parts by weight of the extract of Example 1 of the present invention was added to wheat flour, and the mixture was used to prepare bread, cake, cookies, crackers and noodles.

<2-2> Production of soups and gravies

0.1 to 5.0 parts by weight of the extract of Example 1 of the present invention was added to the soup and the juice to prepare a health improvement meat product, noodle soup and juice.

<2-3> Preparation of ground beef

10 parts by weight of the extract of Example 1 of the present invention was added to ground beef to prepare ground beef for health promotion.

<2-4> Manufacture of dairy products

5 to 10 parts by weight of the extract of Example 1 of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

&Lt; 2-5 >

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a known method, and then they were prepared into powders having a particle size of 60 mesh by a grinder.

The extract of Example 1 of the present invention was concentrated under reduced pressure in a vacuum concentrator, dried by spraying, and dried with a hot air drier, and the dried product was pulverized to a size of 60 mesh with a pulverizer to obtain a dry powder.

The grains, seeds and the extract of Example 1 prepared above were blended in the following proportions.

(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

The extract (3 parts by weight) of Example 1,

(0.5 part by weight),

(0.5 parts by weight)

<2-6> Manufacture of health drinks

5 g of the extract of Example 1 of the present invention was homogeneously mixed with a material such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5% Sterilized, and packaged in glass bottles, plastic bottles, and other small containers.

&Lt; Preparation Example 3 > Preparation of pharmaceutical preparation

<3-1> Manufacture of powder

2 g of the extract of Example 1

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

<3-2> Preparation of tablets

100 mg of the extract of Example 1

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

&Lt; 3-3 > Preparation of capsules

100 mg of the extract of Example 1

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

<3-4> Manufacture of pills

1 g of the extract of Example 1

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

<3-5> Preparation of granules

150 mg of the extract of Example 1

Soybean extract 50 mg

200 mg of glucose

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and dried at 60 캜 to form granules, which were then filled in a capsule.

<3-6> Preparation of Injection

10 mg of the extract of Example 1

Sterile sterilized water for injection

pH adjuster

After mixing the above ingredients, they were prepared with the above ingredient contents (2 ml) per ampoule according to the usual injection preparation method.

&Lt; 3-7 > Preparation of ointment preparation

10 mg of the extract of Example 1

250 mg of PEG-4000

PEG-400 650 mg

White vaseline 10 mg

P-hydroxybenzoic acid methyl 1.44 mg

0.18 mg of p-hydroxybenzoic acid propyl

Purified water quantity

After mixing the above components, an ointment was prepared according to a conventional ointment preparation method.

Claims (12)

A cosmetic composition for whitening comprising the extract of Carthamus tinctorius L. in an amount of 0.0001 to 50% by weight based on the total weight of the composition. 2. The whitening cosmetic composition according to claim 1, wherein the safflower extract is obtained by heating safflower sulfur crushed material with distilled water. 3. The whitening cosmetic composition according to claim 2, wherein the safflower extract is obtained by adding distilled water to the safflower sulfurized powder, heating and extracting it at 60 to 100 DEG C for 1 to 4 hours, followed by filtration, concentration and drying. A composition for whitening whitening characterized by containing 1 to 10% by weight of a safflower sulfur extract based on the total weight of the composition. 5. The whitening food composition according to claim 4, wherein the safflower extract is obtained by heating safflower sulfur crushed material with distilled water. 6. The whitening food composition according to claim 5, wherein the safflower extract is obtained by adding distilled water to the safflower sulfurized product, heating and extracting at 60 to 100 DEG C for 1 to 4 hours, followed by filtration, concentration and drying. A health functional food for preventing or alleviating a pigmentation disease comprising the whitening food composition according to any one of claims 4 to 6. 8. The method according to claim 7, wherein the pigmentation disorder is selected from the group consisting of freckles, senile spots, liver, spots, brown or black spots, daylight pigmentation, cyanic melasma, hyperpigmentation after drug use, sclerotherapy One of post-inflammatory hyperpigmentation, phototoxic response, or other similar small fixed pigmented lesions due to scarring or dermatitis, including scabies and burns, scabies and burns in women who have taken the oral contraceptive pill, sequelae, gravidic chloasma By weight or more. A pharmaceutical composition for preventing or treating pigmentation disorders, which comprises 0.001 to 200 占 퐂 / ml of a safflower extract, based on the total weight of the composition. The pharmaceutical composition according to claim 9, wherein the safflower extract is obtained by heating and extracting safflower sulfur powder with distilled water. 11. The method according to claim 10, wherein the safflower extract is obtained by adding distilled water to the saffron syrup, heat extraction at 60 to 100 DEG C for 1 to 4 hours, followed by filtration, concentration and drying. Or a pharmaceutical composition for therapeutic use. 10. The method of claim 9, wherein the pigmentation disorder is selected from the group consisting of freckles, senile spots, liver, spots, brown or black spots, sunlight pigment spots, blisters hyperpigmentation, hyperpigmentation after drug use, Characterized in that it is at least one of post-inflammatory hyperpigmentation, phototoxic response, or other similar small fixed pigmented lesions due to scarring or scarring, including scarring and burns, in women taking birth control pills, or A pharmaceutical composition for therapeutic use.

Images