KR101509796B1 - Composition for preventing or treating obesity comprising blueberry fermentation extract - Google Patents

Abstract

The present invention relates to a composition for preventing or treating obesity containing a fermented blueberry extract as an active ingredient. The blueberry fermented extract according to the present invention can be used for preventing or treating obesity by decreasing the body weight of mice raised in a high fat diet and by significantly reducing the weight of epididymal peripheral fatty tissue and peripheral fat tissue.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing or treating obesity, comprising a fermented blueberry extract as an active ingredient,

The present invention relates to a composition for preventing or treating obesity containing a fermented blueberry extract as an active ingredient.

Recent changes in diet and eating habits have been accompanied by changes in economic growth and lifestyle. Especially, busy modern people are overweight and obese because of high calorie diet such as fast food and low exercise amount.

Overweight refers to body mass index (BMI, obesity index divided by the square of body weight) between 25 and 30, and obesity is associated with body mass index> 30.

Two-thirds of US adults are overweight or obese, and overweight increases blood pressure and cholesterol levels to increase the incidence of various geriatric diseases such as heart disease, and obesity increases the risk of heart disease, diabetes, arthritis and some cancers .

Overweight and obesity are factors that increase the incidence of various adult diseases such as arteriosclerosis, hypertension, hyperlipidemia or heart disease not only in adults but also in children and adolescents.

Thus, obesity affects all age groups, but especially among the elderly. In the Orient, it is known that obesity was not caused by vegetarianism lifestyle. However, in the modern times, dietary habits changed due to western culture and obesity gradually began to increase in Oriental people as housing environment changed.

In addition, modern people have been reported to suffer from overwork, overeating, stress, etc., about 30-40% of them have obesity, and about 10% of them have serious or pathological obesity.

Obesity is a state of excessive accumulation of fat in the body, indicating a high proportion of fat in body weight, and is classified as simple obesity and secondary obesity (symptom obesity).

Simplicity Causes of obesity include overeating, lack of exercise, and decreased basal metabolism. Most cases of clinical obesity are simple obesity. Simplicity If obesity develops and persists, it causes various health disorders.

Secondary obesity is obesity that occurs due to any underlying disease, including endocrine obesity, hypothalamic obesity, hereditary obesity, or obesity caused by medications.

The main cause of obesity is overgrowth of adipocytes, which is caused by a deficiency of the hormone called leptin, which is secreted by the brain. These deficiencies of leptin hormone are secreted by over-accumulation of animal cholesterol in the body, weakening metabolism in vivo due to overeating, aging, except when genetically deficient.

Obesity itself is a cause of various diseases such as hyperlipemia, hypertension, cardiovascular disease, pseudotumor cerebri, sleep apnea, cancer, pulmonary hypertension, cholecystitis and osteoarthritis Or a decrease in activity. In addition, obesity may be caused by various diseases and may be a complication.

Studies on obesity have been published (Bhabani et al, 2002) in 2002 in India on the separation and purification of HCA from garcinia cambogia (Bhabani et al, 2002) and in the United States in 2005 MCH-R1 (melanin (Palani et al., 2005). In this study, we investigated the effects of dietary supplementation on body weight (BW) in diabetic patients treated with biaryl ureas. In 2005, Japan announced the results of studies on the inhibition of weight gain in rats due to the consumption of soy protein, the decrease of intake, the decrease of triglyceride and the decrease of cholesterol (Tachibana et al, 2005) Studies are progressing steadily.

Drug therapy for treating obesity currently in use includes an appetite suppressant, a fat absorption inhibitor, or a fat burner. Examples of the appetite suppressant include dexflenfluramine, phenylalanine, tyrosine, and methionine. Examples of the fat absorption inhibitor include orlistat and chitosan. Examples of the fat burning agent include sibutramine, ephedrine, caffeine, Cambogia, L-carnitine, glucomannan, galactomannan, chromium picolinate, and the like. However, these obesity treatments may exhibit a temporary weight loss effect, but they may cause side effects such as drug dependency. In addition, there is also a problem that the patient to be administered can not be used continuously for a long period of time because the patient may develop resistance for a short period of time.

Therefore, many researchers are conducting research using herbal medicines to develop an obesity inhibitor that is safe for the human body and has little side effects even after long-term use.

On the other hand, blueberries are shrubs of rhododendrons and rhododendrons. There are about 400 species worldwide, and they are distributed to cold regions such as Africa, Chile, Australia, Japan, USA, Canada, Finland and Sweden. At present, 20 species are known from North America and the most commonly used species are highbush blueberry, V. corymbosum, rabbiteye blueberry, V. Ashei and lowbusy blueberry , V. Angustrafolium). In Korea, there are pure gold trees and wild angel trees. Blueberry fruit is a small bead of the size of a purple bead. The sugar content varies from 11 to 14 ° Brx depending on the varieties. It has a distinctive flavor and aroma, rich in sugars and organic acids, and especially anthocyanins, . Blueberries are known to have the effect of improving vision, retinal degeneration and cataract prevention effect, blood cholesterol and blood glucose lowering effect, and reduction of bone density. In addition, the blueberry has an anticancer effect (Seeram NP, Adams LS, Zhang Y, Lee R, Sand D, Scheuller HS, Heber D. 2006, Blackberry, black raspberry, blueberry, cranberry, red raspberry, apoptosis of human cancer cells in vitro. J Agric Food Chem., Dec 13: 54 (25): 9329-39). On the other hand, the obesity-inducing effect of blueberry on the high fat diet (Prior RL, Wu X, Gu L, Hager TJ, Hager A, Howard L R. 2008, Whole berries versus berry anthocyanins: interactions with dietary fat levels in the C57BL / 6J J Agric Food Chem., Feb 13; 56 (3): 647-653. Epub 2008 Jan 23). Table 1 shows the results of obesity induction by blueberry ingestion in high fat diet.

Obesity induced by blueberry ingestion in high fat diet Dietary processing Weight (g) Fat tissue (%) Low fat Control group 27.0 ± 0.8 18.0 ± 1.5 Blueberries 26.9 ± 0.5 17.7 ± 1.3 High fat Control group 32.6 ± 1.4 27.5 ± 1.4 Blueberries 37.2 ± 0.9 35.1 ± 1.0

 Therefore, the consumption of blueberry fresh fruits to modern people who do the high-fat diet, there is a concern that deepening obesity.

The inventors of the present invention have found that blueberry fermented extract reduces the body weight of mice raised in a high fat diet while developing a drug that is harmless to human body and has the effect of preventing or treating obesity, And the weight of the adipose tissue was significantly reduced, thereby completing the present invention.

The present invention is intended to provide a composition for preventing or treating obesity containing a fermented blueberry extract as an active ingredient.

The present invention provides a composition for preventing or treating obesity containing a fermented blueberry extract as an active ingredient.

The composition comprises a pharmaceutical composition and a food composition.

Hereinafter, the present invention will be described in detail.

The blueberry fermented extract as an active ingredient of the composition of the present invention can be obtained by fermenting blueberry with a conventional method to obtain a fermented blueberry dried product and then extracting the fermented blueberry dried product by a conventional method. Typical extraction methods include, but are not limited to, ultrasonic extraction, filtration, and reflux extraction.

In the present invention, the blueberry fermentation extract can be obtained by fermentation, extraction and separation as follows. First, the blueberries are washed, dried and then ground. The pulverized blueberries are fermented by a conventional method. The yeast used in the fermentation is preferably Saccharomyces cerevisiae, but is not limited thereto. The fermentation temperature is 10 to 30 占 폚, the fermentation period is 3 to 15 days, preferably 1 week. In order to promote fermentation, about 5 to 30% of sugar, glucose and the like may be added. After the fermentation is completed, the fermentation liquid is dried by hot air drying or freeze drying to obtain a fermented blueberry dried material. The above-obtained fermented blueberry fermentation product is dipped in a mixed solvent of water, a C ₁ to C ₄ alcohol or water and a C ₁ to C ₄ alcohol, and extracted with stirring at a speed of 100 to 350 rpm. At this time, the volume of the solvent is 5 to 15 times the weight of the pulverized sample, and the extraction time is 1 to 5 days, preferably 3 days. The C ₁ -C ₄ alcohol may be selected from methanol or ethanol, preferably ethanol. Thereafter, the extract is filtered, and the solvent of the resulting supernatant is concentrated under reduced pressure to obtain a blueberry fermented extract.

The blueberry fermented extract according to the present invention reduces the body weight of the mice raised in the high fat diet and significantly reduces the weight of the epididymal and adipose tissue. Therefore, the blueberry fermented extract according to the present invention can be used as medicines and health foods useful for prevention or treatment of obesity.

The composition of the present invention may contain at least one known active ingredient having the effect of preventing or treating obesity together with the blueberry fermented extract.

The composition of the present invention may further comprise at least one pharmaceutically acceptable carrier in addition to the above-described effective ingredients for administration. The pharmaceutically acceptable carrier may be a mixture of saline, sterilized water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components. If necessary, an antioxidant, , And other conventional additives such as a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Further, it can be suitably formulated according to each disease or ingredient, using appropriate methods in the art or by the method disclosed in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA.

The composition of the present invention may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) depending on the intended method, and the dose may be appropriately determined depending on the patient's weight, age, , Diet, administration time, method of administration, excretion rate, and severity of the disease. The daily dose of the blueberry fermented extract is about 0.1 to 100 mg / kg, preferably about 30 to 80 mg / kg, more preferably about 50 to 60 mg / kg, and is administered once a day to several times a day .

The composition of the present invention can be used alone or in combination with methods for the prevention or treatment of obesity or using surgery, hormone therapy, drug therapy and biological response modifiers.

The composition of the present invention may be added to a health food for the purpose of obesity suppression. When the blueberry fermented extract of the present invention is used as a food additive, the blueberry fermented extract may be added as it is or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, in the production of foods or beverages, the blueberry fermented extract of the present invention is added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

Example 1  : Preparation of Blueberry Fermented Water Extract

1. Preparation of fermented blueberry dried product

The blueberries were cleanly washed with water, dried and then ground. The pulverized blueberries were fermented by a conventional method. The yeast used in the fermentation was Saccharomyces cerevisiae, the fermentation temperature was 10 to 30 ° C, and the fermentation period was one week. In order to promote fermentation, about 5 to 30% of sugar, glucose and the like may be added. After completion of the fermentation, the fermentation broth was dried by hot air drying or freeze-drying to obtain a blueberry fermentation product.

2. Preparation of Blueberry Fermented Water Extract

0.1 kg of the fermented blueberry fermentation product prepared in 1 above was placed in an extraction container, and 10 times water was added to the weight of the fermented blueberry fermentation product, followed by immersion for 3 days. The extract was filtered, and the solvent of the resulting supernatant was concentrated under reduced pressure to obtain 54.3 g (yield: 54.3% (w / w)) of blueberry fermented water extract.

Example 2  : Preparation of Blueberry Fermented Ethanol Extract

0.1 kg of the dried fermented blueberry prepared in Example 1 was placed in an extraction container, and 10 times 100% ethanol (HPLC grade) was added to the weight of the fermented blueberry fermentation product. The fermented product was immersed in the fermentation broth for 3 days at room temperature, (180 rpm) (3 repetitions). The extract was filtered, and the solvent of the resulting supernatant was concentrated under reduced pressure to obtain 23.5 g (yield: 23.5% (w / w)) of blueberry fermented ethanol extract.

Example 3  : Preparation of Blueberry Fermented Methanol Extract

0.1 kg of the fermented blueberry fermentation product prepared in Example 1 was placed in an extraction container, 10 times 100% methanol (HPLC grade) was added to the weight of the fermented blueberry fermentation product, and the mixture was immersed at room temperature for 3 days. (180 rpm) (3 repetitions). The extract was filtered, and the solvent of the resulting supernatant was concentrated under reduced pressure to obtain 31.6 g (yield 31.6% (w / w)) of ethanol extract of fermented blueberry.

Experimental Example 1  : Weight change and weight measurement of adipose tissue

In order to confirm the effect of the fermented blueberry extract according to the present invention on weight change and weight of adipose tissue, the following experiment was conducted.

1. Weight change measurement

Male ICR mice (Orient Bio, Korea) were divided into three groups (normal diet (control group), high fat diet, high fat diet including blueberry fermented extract) for 4 weeks after birth and 5 rats were assigned to each group. The experimental animals were fed with the AIN-93 diet diet, the high-fat diet (45% increase in calorie), and the free consumption of drinking water for 30 days. In the high-fat diet containing blueberry fermented extract, the blueberry fermented ethanol extract prepared in Example 2 was mixed in a high fat diet at a ratio of about 1%, and the diet was fed at a feed rate of 4 g / day for one day, once a day for 30 days. Table 2 shows the composition of the AIN-93 diet (%) and the composition of the high-fat diet (%). Changes in body weight of mice fed the high fat diet were measured every week from the start of the experiment. Body weight changes are shown in Fig.

AIN-93 diet diet and high fat diet (%) ingredient (%) Highland method (%) Casein 14.0 14.0 L-cysteine 0.18 0.18 Corn starch 45.77 45.77 Luxurious corn starch 15.5 15.5 Sugar 10.0 0.0 fibrin 5.0 5.0 Soybean oil 4.0 4.0 AIN093 comprehensive mineral 3.5 3.5 AIN093 multivitamin 1.0 1.0 Hydrogen stearate choline 0.25 0.25 t-butylhydroquinone 0.8 0.8 lard 0.0 10.0

As shown in FIG. 1, the high-fat diet group containing the fermented blueberry extract according to the present invention showed a remarkable weight loss effect as compared with the high fat diet group.

2. Weighing of fatty tissue

The weight of adipose tissue in the mice fed the high fat diet was measured after incising epididymal fat tissue and perirenal fat tissue. The weight of the epididymal adipose tissue and the adipose tissue around the kidney are shown in FIG. 2 and FIG. 3, respectively.

As shown in FIG. 2 and FIG. 3, the weight of the epididymal perihepatic fat tissue and the peripheral fat tissue of the high fat diet group containing the fermented extract of blueberry according to the present invention was significantly lower than that of the high fat diet group.

Examples of formulations for the composition of the present invention are illustrated below.

Formulation Example 1  : Preparation of pharmaceutical preparations

1. Manufacturing of powder

Blueberry fermented extract 2g

Lactose 1g

The above components were mixed and packed in airtight bags to prepare powders.

2. Preparation of tablets

Blueberry fermented extract 100mg

100 mg of corn starch

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of capsules

Blueberry fermented extract 100mg

100 mg of corn starch

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

Formulation Example 2  : Manufacturing of food

Foods containing the blueberry fermented extract of the present invention were prepared as follows.

1. Preparation of cooking seasoning

And 20 to 95% by weight of a blueberry fermented extract to prepare a cooking sauce for health promotion.

2. Manufacture of tomato ketchup and sauce

0.2 to 1.0% by weight of blueberry fermented extract was added to tomato ketchup or sauce to prepare healthy tomato ketchup or sauce.

3. Manufacture of flour food

0.5 to 5.0% by weight of blueberry fermented extract was added to wheat flour, and bread, cake, cookies, crackers and noodles were prepared by using this mixture to prepare health improving foods.

4. Manufacture of soups and gravies

0.1 to 5.0% by weight of blueberry fermented extract was added to soup and juice to prepare health promotion meat product, noodle soup and juice.

5. Manufacture of ground beef

10% by weight of blueberry fermented extract was added to ground beef to prepare ground beef for health promotion.

6. Manufacture of dairy products

5 to 10% by weight of fermented blueberry extract was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

Formulation Example 3  : Manufacture of beverages

1. Manufacture of carbonated beverages

The syrup is prepared by mixing 5 to 10% of sugar, 0.05 to 0.3% of citric acid, 0.005 to 0.02% of caramel and 0.1 to 1% of vitamin C and 79 to 94% of purified water to prepare syrup, ° C for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 4, and 0.5 to 0.82% of carbon dioxide gas was injected to prepare carbonated beverages containing the blueberry fermented extract of the present invention.

2. Manufacture of health drinks

The ingredients such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%) and the blueberry fermentation extract were blended homogeneously, Bottles, plastic bottles, etc.

3. Manufacture of vegetable juice

Healthy vegetable juice was prepared by adding 5 g of blueberry fermented extract to 1,000 ml of tomato or carrot juice.

4. Manufacture of fruit juice

1 g of the fermented blueberry extract was added to 1,000 ml of apple or grape juice to prepare health promotion fruit juice.

The blueberry fermented extract according to the present invention can be used for preventing or treating obesity by decreasing the body weight of mice raised in a high fat diet and by significantly reducing the weight of epididymal peripheral fatty tissue and peripheral fat tissue.

Brief Description of the Drawings Fig. 1 is a graph showing a weight loss effect of a fermented blueberry extract according to the present invention. Fig.

FIG. 2 is a graph showing a weight-reducing effect of the epididymal peripheral fat tissue of the blueberry fermentation extract according to the present invention.

FIG. 3 is a graph showing a weight-reducing effect of fat surrounding fat tissue of a fermented blueberry extract according to the present invention.

Claims (12)

A pharmaceutical composition for the prevention or treatment of obesity containing an extract of fermented blueberry as an active ingredient, Wherein the blueberry fermentation extract is an extract of fermented blueberry obtained by culturing a strain of the genus Saccharomyces in blueberry. According to claim 1, wherein the blueberry fermentation extract is Saccharomyces as MY process in then using the strains ferment blueberry was obtained blueberry fermentation dried material dried and then purified by the blueberry fermentation dried product, C ₁ ~ C ₄ Of an alcohol or a mixture of water and a C ₁ to C ₄ alcohol. delete The composition according to claim 2, wherein the fermentation is carried out at 10 to 30 DEG C for 3 to 15 days. The composition of claim 2, further comprising sugar or glucose during fermentation. The composition according to claim 2, wherein the C ₁ -C ₄ alcohol is selected from methanol or ethanol. A food composition for inhibiting obesity containing a fermented blueberry extract as an active ingredient, Wherein the blueberry fermentation extract is an extract of fermented blueberry obtained by culturing a strain of the genus Saccharomyces in blueberry. Claim 7, wherein the method, the blueberry fermentation extract is Saccharomyces as MY process in then using the strains ferment blueberry dried to obtain a blueberry fermented dry matter, and then purified by the blueberry fermentation dried product, C ₁ ~ C ₄ Of an alcohol or a mixture of water and a C ₁ to C ₄ alcohol. delete 9. The composition of claim 8, wherein the fermentation is performed at 10 to 30 DEG C for 3 to 15 days. The composition of claim 8, further comprising sugar or glucose during fermentation. The composition of claim 8, wherein the C ₁ -C ₄ alcohol is selected from methanol or ethanol.

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