KR101416673B1 - Ginseng prosapogenin high concentration containing ginseng flower preparation using sonication and process for thereof - Google Patents

Abstract

The present invention relates to a ginseng root preparation containing high concentration of ginsenosides Rg ₂ , Rh ₁ and F ₄ using ultrasonic treatment and a method for producing the ginseng root preparation. The ginseng root is maintained at a temperature within a range of 1 minute to 60 minutes, Ginseng rosin containing high concentrations of ginsenosides Rg ₂ , Rh ₁ and F ₄ , which are functional substances produced by heat in the production of red ginseng by ultrasonication at a temperature and drying the ultrasonic treated ginseng roaster, ≪ / RTI >

Description

[0001] The present invention relates to a ginseng prosapogenin containing high concentration of ginseng prosapogenin and a method for producing the same,

The present invention relates to a ginseng flavor preparation and a method for producing the ginseng flavor, and in particular, it is possible to increase the content of prosapogenin, which is a functional substance produced by heat in the production of red ginseng by ultrasonication.

Korean ginseng ( Panax ginseng ) is a representative product of Korea, which is a summary of Daebo- boo , which is handled by products of sacred Maternity, menstrual cycle , taste is slightly warm, KOREA INSAM is a physiologically active substance which contains 30 kinds of ginseng saponins including ginsenoside Rb ₂ having anti-diabetic action, polyacetylene having anticancer action, phenolic compound having antioxidant activity, ginseng protein having radiation- , An acidic polysaccharide having an immunoregulatory action, and the like. In particular, Korean ginseng contains a larger amount of phenolic compounds, polyacetylenes and acidic polysaccharides than Panax quinquefolium , so that stronger physiological activity is expected. The ginseng saponin, which is known as the main pharmacological component of Korean ginseng, is called Jinseong - said, and its chemical structure has been confirmed by the Shibata group of Tokyo University. As such, the ginseng saponin contained in KOREA INSAM is 30 species, 14 species of western ginseng and 13 species of Panax notoginseng ) than 15 species. The ginsenosides are broadly divided into the protopanaxdiol group and the protopanaxtriol group. The major component of the protopanaxadiol group is ginsenoside Rb ₁ , which exhibits a central nervous inhibitory action , A major component of the protopanaxyl triol group, is shown to exhibit central nerve stimulation by ginsenoside Rg ₁ and is believed to contribute mainly to the adaptogen activity of Korean ginseng.

The content ratio (Rb ₁ / Rg ₁ ) of ginsenoside Rb ₁ to ginsenoside Rg ₁ (Rb ₁ / Rg ₁ ) in the Korean ginseng was 1.96 and that of protopanaxadiol and protopanaxatriol 3.14, showing a more balanced and more exciting, rather than exciting, visual action, as compared with 2.48 and 25.96 of the western ginseng exhibiting excessive central nervous inhibitory action. Therefore, the Korean ginseng has a pharmacological effect, and it has a true meaning, that is, the ginsenoside Rb ₁ of the inner spirit action and the ginsenoside Rg ₁ of the inner gesture are balanced, It seems to be the best tonic medicine of modern people in.

The pharmacological activities of Korean ginseng so far confirmed include reinforcement of heart and blood vessels, recovery of blood function, improvement of blood flow, prevention of atherosclerosis and hypertension, strengthening of gastrointestinal function, promotion of liver function and elimination of hangover, The present invention relates to a pharmaceutical composition for treating or preventing anxiety or depression of a person suffering from or at risk of developing a disease, Enhancement of immunity, inhibition of AIDS virus proliferation, and promotion of protein synthesis ability.

Also, it is said that dried red ginseng, peel, and roots are dried and dried naturally in the sunlight. Particularly, red ginseng is attracted attention as a special feature of red ginseng because of its specific action such as ginsenosides Rg _{3 ,} Rg _{5 , and} Rh _{1 ,} which are generated by heat and have a small amount _{, have} antitumor activity, antitumor activity, hypotensive action and antioxidant action .

Ginseng has been regarded as the best brand for a long time, but now it has a market share of 3% in the world ginseng market. In order to innovate such ginseng industry, it is necessary to develop high value-added ginseng products Do.

Nevertheless, the ginseng and red ginseng preparations so far have been developed at the level of the tonic to form the mainstream extract. However, in order to develop more specialized and functional ginseng preparations, it is essential to develop ginseng preparations containing a high concentration of a functional substance with a high level of physiological activity and safety.

For these reasons, recently, methods for making ginseng preparations containing a large amount of ginsenoside Rg ₃ having excellent effects among components of ginseng have been attempted.

According to the Shibata paper published in 1966, when saponin is hydrolyzed to a weak acid such as acetic acid, only glucosidic bonds of C-20 (ginsenosides Rb ₁ , Rb ₂ , Rc and Rd) (R & S) - Ginsenoside Rg ₃ ) was obtained, but this only resulted in the production of standard products. In addition, other researchers have used high-temperature treatment, which is a physical reaction, or enzymatic treatment, which is a biochemical reaction, in order to produce a ginseng preparation containing a large amount of ginsenoside Rg ₃ .

The ginseng is heat-treated at a high temperature of 120 to 180 ° C for 0.5 to 20 hours to obtain ginsenoside (Rg ₃ + Rg ₅ ). The ginseng is heated at a high temperature, for example, Wherein the ratio of (Rc + Rd + Rb ₁ + Rb ₂ ) is greater than or equal to 1, and a patent for beverage composition contained in the processed food (Korean Patent No. 192678) and saponin glucoside degrading enzyme (Korean Patent No. 329259) on a method for producing rare-anticancer saponins (Rh ₁ , Rh ₂ ) by hydrolysis of the saponin sugar, and they are commercially available as sansam and sinsam in the market. However, this method increases the efficacy, but it takes a long time to manufacture, requires special equipment such as a high-pressure heater, and particularly requires heating treatment at a temperature higher than normal processing temperature, so that ginseng is carbonized when mass- There are many operational difficulties in the manufacturing process.

(Japanese Patent No. 228510) and a blood vessel relaxant composition (Korean Patent No. 201585), ginsenosides Rg ₃ and / or Rg ₅ are produced under mild conditions instead of heat treatment at a high temperature, for example, An effective component such as Rg ₃ is produced in the same manner as in the reaction condition of heating treatment at a high temperature even when the acid is treated with a lower organic acid such as acetic acid, tartaric acid, oxalic acid or the like, but using a lower organic acid such as acetic acid or citric acid As a result of the experiment, it was confirmed that it was difficult to prepare ginseng preparations containing Rg ₃ and Rg ₅ , and a large amount of impurities were contained in the analysis of the components (Korean Patent No. 635025).

Examples of techniques using conventional vinegar include a ginseng preparation using vinegar and a preparation method thereof (Korean Patent No. 635025), a process for producing red ginseng vinegar mixed with ginseng and red ginseng (Korean Patent No. 244849), a method for producing ginseng vinegar (Korean Patent No. 344949), which is intended to produce vinegar by mixing only trace amounts of ginseng or red gins into vinegar, or a method for manufacturing ginseng preparations containing ginsenoside Rg _{3 at a} high concentration.

Accordingly, the present inventors have conducted continuous research to produce a ginseng preparation containing a high concentration of a functional substance such as ginsenosides Rg ₃ , Rg ₅ , Rh _1, Rh _4, Rk ₁ and F ₄ , (For example, ginsenosides Rg ₃ , Rg ₅ , Rh _1, Rh _4, Rh _2, and Rh ₃₎ which are functional materials that are produced in a small amount by heat during the production of red ginseng by irradiating ultrasonic waves to the ginseng roulade _, Rk ₁ and F ₄ ) at a high concentration.

It is another object of the present invention to provide a ginseng roasted preparation containing the prosapogenin component at a high concentration and a method for economical production thereof.

In order to accomplish the above object, the present invention provides a method for preparing a ginseng root, comprising: 1 part by weight of ginseng root into 5 to 300 parts by weight of distilled water and sonicating at a temperature within a range of 90 to 110 ° C for a time within a range of 40 to 50 minutes; And a step of concentrating and freeze-drying the ultrasonic treated ginseng roots under reduced pressure, wherein the content of ginsenosides Rg ₃ , Rg ₅ , Rh _1, Rh _4, Rk ₁ and F ₄ is increased This is a method for producing ginseng roast formulations.

Here, it is preferable to use an ultrasonic wave generator of 600 W to perform the ultrasonic wave processing.

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On the other hand, another embodiment of the present invention is a process for producing a ginsenoside Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg 1, Rg 2, Rg 3, Rg 5, Rg 6, Rh 1, Rh 4, Rk 1, Rk 3, F 1 and binary respect to the sum of F ₄ ginsenoside Rg _2, Rh ₁ and F ₄ are contained in the range of 65 wt% to 85 wt%.

Wherein the ginseng root is an extract of ginseng root, and the ginsenoside Rg ₂ , Rh ₁ and F ₄ are contained in the range of 1 wt% to 10 wt% with respect to the ginseng root extract. It may be the first.

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The details of other embodiments are included in the detailed description and drawings.

As described above, the present invention relates to a process for producing red ginseng by ultrasonication of a ginseng roaster, wherein the ginsenoside Rs ₃ , Rg ₅ , Rh _1, Rh _4, Rk ₁ And F ₄ ) can be increased.

In addition, the present invention can provide a method for easily manufacturing a ginseng roasted preparation containing a high concentration of the prosapogenin component by adding ginseng roots into distilled water and subjecting the roasted ginseng to ultrasonic treatment.

Figure 1 is an HPLC chromatogram of a standard authentic ginsenoside component.
FIG. 2 is an HPLC chromatogram of a ginsenoside component of a ginseng roughened formulation prepared by a method of ultrasonication for 10 minutes according to an embodiment of the present invention (Example 1)
FIG. 3 is an HPLC chromatogram of a ginsenoside component for a ginseng roughened preparation prepared without an ultrasonic treatment according to Comparative Example 1 of the present invention (Comparative Example 1).

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The present invention relates to a method for producing ginseng roots, comprising the steps of: sonicating ginseng rosewood berries for a period of time ranging from 1 minute to 60 minutes; And drying the ultrasonic treated ginseng root. Containing ginseng root preparation.

More particularly, the present invention relates to a method for preparing a ginseng flavor preparation to be subjected to ultrasonic treatment, wherein a ginseng prosapogenin component (for example, ginsenoside Rg ₃ , Rg ₅ , Rh _1, Rh _4, Rk ₁ and F ₄ ) at a high concentration.

As described above, the present invention is characterized in that the ultrasound is used to maximize the change of the active ingredient in the ginseng root and the content of the ginseng rare ingredient.

The manufacturing method according to the present invention will be described in more detail as follows.

First, the ginseng roaster is subjected to ultrasonic treatment for a time within a range of 1 minute to 60 minutes.

The ginseng includes all kinds of ginseng belonging to the technical field of the present invention. The ginseng that can be used in the present invention is Korean ginseng ( Panax ginseng ), Panax quinquefolium , Panax notoginseng , Panax japonicum) or Panax, for Vietnam N-Sys (Panax vietnamensis) and Panax (Panax) hwaroe crude drugs of the genus plant, such as for example, seoyangsam hwaroe, intended to also include the case a samchil three hwaroe, jukjeol three hwaroe and those processed, which ginseng hwaroe Based on facts confirmed from repeated experiments involving Preferably, ginseng roots of Korean ginseng can be used. In addition, the present invention is also applicable to the above-mentioned ginseng flesh extract (ginseng flesh extract).

In addition, the present invention is characterized in that the ginseng roaster is ultrasonicated for a period of time ranging from 1 minute to 60 minutes. The ultrasonic treatment can be performed by various treatment methods known in the art. For example, it is possible to use an ultrasonic generator of an oscillator 600W and a vibration unit 600W. The ultrasonic treatment is preferably performed for a time within a range of 1 minute to 60 minutes and more preferably within a range of 5 to 50 minutes and more preferably within a range of 40 to 50 minutes .

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In another embodiment of the present invention, the ultrasonic treatment is carried out at a high temperature within a range of 90 ° C to 110 ° C, and thus the ginseng roughening agent having an increased drug efficacy can be prepared by extracting at such a high temperature.

In another embodiment of the present invention, in performing the ultrasonic treatment, the ginseng root is placed in distilled water and treated with ultrasonic waves. That is, it is intended to transmit ultrasound more effectively and stably to the ginseng roaster through distilled water. When the ginseng roast is put into distilled water and subjected to ultrasonic treatment, it is more preferable to add 1 part by weight of ginseng roots into 5 to 300 parts by weight of distilled water. If the amount of distilled water is less than the above range, the ultrasonic effect is insufficient, This is because distilled water may cause a change in useful components.

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Then, the present invention comprises a step of drying the ultrasonic treated ginseng root. The time or method of drying is not particularly limited, but it is possible to dry the ultrasonic treated ginseng roaster at a temperature within the range of 30 ° C to 50 ° C for a period of time ranging from 1 day to 3 days in a vacuum dryer. In the drying step, it is more preferable that the sonicated ginseng roaster is concentrated under reduced pressure and freeze-dried.

Thus, treatment of ultrasonic waves at about 100 ° C. for 1 minute to 60 minutes by adding distilled water to the ginseng roaster resulted in the contents of Rg ₃ , Rg ₅ , Rh _1, Rh _4, Rk ₁ and F ₄ as useful ginsenoside components A high ginseng preparation can be produced.

In addition, the manufacturing method of the present invention may further include a post-processing step. The processing may be performed by a known method to prepare a processed ginseng extract. That is, the ginseng is extracted by using water, a lower alcohol such as methanol or ethanol, a lower ketone such as acetone or methyl ethyl ketone, a supercritical fluid or a mixed solvent thereof, To remove the solvent to prepare a dried powdery processed ginseng extract, that is, ginseng, in the form of a soft or dry extract.

In another embodiment of the present invention, there is provided a process for producing a prosapogenin composition, which comprises preparing a prosapogenin component which is produced by the above-described production method and comprises ginsenoside Is a ginseng flavor preparation.

Here, the ginsenosides are ginsenosides Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg 1, Rg 2, Rg 3, Rg 5, Rg 6, Rh 1, Rh 4, Rk 1, Rk 3, F 1 and binary respect to the sum of F ₄ ginsenoside Rg _2, It is preferable that Rh ₁ and F ₄ are contained at a high concentration in the range of 65 wt% to 85 wt%.

In particular, the functional material Rg ₂ 4.7 to 6.0%, Rh ₁ 1.4 to 1.9%, and F _4: 1.2 to 1.5% at a high concentration.

That is, the ginseng rosemary preparation according to the present invention, which is manufactured by the above method and contains ginseng prosapogenin at a high concentration, Improvement of metabolic syndrome, anticancer, blood pressure lowering, sexual function improvement, liver function improvement, brain cognitive function improvement, ADHD improvement, atopy and allergy improvement.

Furthermore, the total daily dose to be administered to the host at a single dose or two to three separate doses for the clinical purpose of the ginseng roughened preparation of the present invention is preferably in the range of 1 to 50 mg per 1 kg body weight, May be varied depending on the particular compound being used, body weight, age, sex, health condition, diet, time of administration, method of administration, excretion rate, drug mix and severity of the disease.

The compounds of the present invention may be administered as injectable preparations and oral preparations as desired. Injectable preparations, for example sterile injectable aqueous or oleaginous suspensions, may be prepared according to the known art using suitable dispersing agents, wetting agents or suspending agents. Solvents that may be used herein include water, Ringer's solution and isotonic NaCl solution, and sterile, fixed oils are typically used as a solvent or suspending medium. Any non-irritating fixed oils, including mono-, di-glycerides, may be used for this purpose, and fatty acids such as oleic acid may be used in injectable preparations. Dosage forms for oral administration include capsules, tablets, pills, powders, granules and solutions, and particularly capsules, tablets and liquids are useful. Tablets and pills are preferably prepared as preservative. Solid and liquid dosage forms can be prepared by mixing the active ingredient with one or more inert diluents such as sucrose, lactose, starch and the like, lubricants such as magnesium stearate, talc, disintegration aids such as calcium CM, binders, fragrances, preservatives such as sodium benzoate , Sweeteners such as sugars or fructose, and surfactants. More specifically, a capsule may be prepared by adding starch and lactose as an excipient at a ratio of 7: 3 as an excipient, and adding magnesium stearate and talc at 3% or less for increasing fluidity. The starch and lactose Is added at a ratio of 7: 3, followed by tableting with calcium carbonate as a binder and disintegration aid. Flavor as a fragrance, sodium benzoate as a preservative, sugar or fructose as a sweetener, and a surfactant are added to a liquid Can be produced.

The present invention may be better understood by the following examples, which are for the purpose of illustrating the invention and are not intended to limit the scope of protection defined by the appended claims.

Manufacturing example  : Manufacture of ginseng roe extract

Five hundred milligrams of ethyl alcohol (Ethyl alcohol) was added to each 500 g of dried ginseng roots, and the mixture was refluxed four times, filtered and concentrated under reduced pressure to obtain ethyl alcohol ginseng fuller's extract.

Example  1 ~ 7: Preparation of ginseng roots preparation using ultrasonic

Example  1: Ultrasonication treatment with distilled water for 10 minutes to prepare ginseng roughened preparations

200 ml of distilled water was added to 1 g of the ginseng rosemary extract obtained in the above Preparation Example, and the mixture was placed in a sonicator of 600 W, 600 W, and 100 캜, and sonicated for 10 minutes. Then, it was concentrated under reduced pressure and then lyophilized to obtain a brownish extract.

Example  2: Ultrasonication with distilled water for 20 minutes to prepare ginseng roughened preparation

The extract was obtained by the method of Example 1, except that the ultrasonic treatment was carried out for 20 minutes in the above Example 1.

Example  3: Ultrasonic treatment for 30 minutes with distilled water to prepare ginseng roasted preparations

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 30 minutes in the above-mentioned Example 1.

Example  4: Ultrasonic treatment for 40 minutes with distilled water to prepare ginseng roast preparations

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 40 minutes in Example 1.

Example  5: Ultrasonic treatment with distilled water for 50 minutes, to prepare ginseng roasted preparations

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 50 minutes in the above-mentioned Example 1.

Example  6: Ultrasonic treatment for 60 minutes with distilled water to prepare ginseng rosin preparation

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 60 minutes in Example 1.

Example  7: Ultrasonication with vinegar for 40 minutes, preparation of ginseng rosin preparation

An extract was obtained in the same manner as in Example 1, except that the vinegar was used in place of the distilled water in Example 1 and ultrasonicated for 40 minutes.

Comparative Example  1: Preparation of ginseng roaster without ultrasonic treatment

To 500 g of dried ginseng blend, 2,500 ml of ethyl alcohol was added, and the mixture was refluxed twice, filtered and concentrated under reduced pressure to obtain ethyl alcohol ginseng fullerenes. Then, the obtained ginseng rosemary extract was lyophilized to obtain a brownish extract.

Experimental Example  : HPLC Through law Gin Senocide  Component analysis

① Experimental method

50 ml of diethylether was added to 2 g of each of the ginseng root extract preparations obtained in Examples 1 to 7 and Comparative Example 1, and the mixture was centrifuged three times for 1 hour, and then the supernatant was removed.

Subsequently, 50 ml of butanol saturated with water was added to the obtained residue, and the mixture was extracted three times for 2 hours. The resulting mixture was centrifuged, and the supernatant was filtered and concentrated under reduced pressure to obtain crude saponin. The thus obtained crude saponin was quantified by HPLC method, and the results are shown in Tables 1 to 3 below.

② HPLC analysis conditions

The results obtained by the above-mentioned experimental method were directly compared with the specimens. That is, the content and the composition of ginseng saponin were repeatedly tested three times per each sample to confirm the reproducibility of the results. The product used was ginsenoside of 99% or more purity purchased from Chromadex (U.S.A.).

The HPLC apparatus used was a Waters 1525 binary HPLC system (Waters, USA) and a column was Eurospher 100-5 C18 (250 * 3 mm).

The mobile phase was acetonitrile (HPLC grade, Sigma, USA) and distilled water for HPLC. The ratio of acetonitrile was changed from 17% (0 min) to 30% (55 min), 40% (80 min), 60% % (140 min) and finally adjusted to 17%. The development temperature was measured at room temperature, the flow rate was 0.8 ml / min, and the chromatogram was detected at 203 nm using uv / vis Waters 2487 Dual λ Absorbance Detector (Waters, USA) detector.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is an HPLC chromatogram of a standard authentic ginsenoside component wherein the designation of each symbol represents the following ginsenoside;

  a: Ginsenoside Rg1, b: Ginsenoside Re, c: Ginsenoside Rf,

     d: Ginsenoside Rb1, e: Ginsenoside Rg2, f: Ginsenoside Rh1,

   g: Ginsenoside Rc, h: Ginsenoside Rb2, i: Ginsenoside F1,

    j: Ginsenoside Rd, k: Ginsenoside Rg6, l: Ginsenoside F4,

   m: Ginsenoside Rk3, n: Ginsenoside Rh4 o: Ginsenoside (20S) Rg3,

p: Ginsenoside (20R) Rg3, q: Ginsenoside Rk1, r: Ginsenoside Rg5.

FIG. 2 is an HPLC chromatogram of a ginsenoside component of a ginseng roughened preparation prepared by a method of ultrasonication for 10 minutes according to an embodiment (Example 1) of the present invention. (Comparative Example 1) of the ginseng root preparation prepared without the ultrasonic treatment.

As shown here, the sample was directly compared with the sample through HPLC, and the average was statistically calculated.

Table 1 below shows the ginsenoside content of the ginseng root extract obtained by the above Examples 1 to 7 and Comparative Example 1.

As shown in Table 1, analysis of ginseng saponin ginsenosides Rb _1, Rb _2, Rc, Rd, Re, Rf, Rg _1, Rg _2, Rg _3, Rg _5, Rg _6, Rh _1, Rh ₄ , Rk ₁ , Rk ₃ , F ₁ and F ₄ , and the prosapogenin components (ginsenosides Rg ₂ , Rh ₁ and F ₄ ) useful in Examples 1 to 7 of the present invention were highly confirmed.

Particularly, in the case of ginsenoside Rh ₁ in which ginsenoside Rg ₂ can be produced by hydrolysis, the content of ginsenoside Rh ₁ was the highest at 1.85% in Example 4 (treated with distilled water for 40 minutes) Treated with distilled water for 50 minutes) showed a content of 1.78%.
And, ginsenoside F ₄ showed the highest content of 1.53% in Example 5, and 1.52% in Example 4, respectively.
Further, in Examples 4 and ₅ , the contents of Rg ₂ , Rg ₃ , Rg ₅ , Rh _1, Rh _4, Rk ₁ and F ₄ were significantly superior to those of Comparative Examples and other Examples.
However, the content of ginsenoside Rg ₂ showing the efficacy of wrinkle-improving action and anti-thrombotic action showed the highest content as 6.0% in Example 1, and the content of ginsenoside Rg ₂ in Example 7 (5.72%) and Example 4 %), And the content of Example 1 was not confirmed in Comparative Example 1.

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While the present invention has been particularly shown and described with reference to preferred embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be apparent to those skilled in the art that changes may be made.

Claims (12)

Adding 1 part by weight of ginseng root into 5 to 300 parts by weight of distilled water and sonicating at a temperature within a range of 90 to 110 캜 for a time within the range of 40 to 50 minutes; And
And concentrating and lyophilizing the ultrasonic treated ginseng root under reduced pressure,
Wherein the content of ginsenosides Rg ₃ , Rg ₅ , Rh _1, Rh _4, Rk ₁ and F ₄ is increased.
delete delete The method according to claim 1,
Wherein the ultrasound treatment is performed using an ultrasonic generator of 600W.
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