KR100261045B1 - Method for extracting a beta-glucan from a grain - Google Patents

Abstract

PURPOSE: Provided is a method for simply extracting beta-glucan from grains at high yields. CONSTITUTION: The method for extracting beta-glucan comprises the steps of: grinding grains containing beta-glucan such as barley, rye or oats; adding hot water into the grain powder; agitating the grain mixture for 1 to 2 hours and centrifuging it; collecting the supernatant therefrom and heating it at 60 to 100 deg. C to deactivate beta-glucanase and coagulate proteins; adding alpha-amylase into the solution and cooling it; filtering the solution to remove coagulated proteins; adding alcohol into the filtrate and slowly agitating the solution to precipitate solid components; and centrifuging the solution and removing the supernatant to collect the solid beta-glucan.

Description

Β-glucan extraction method of cereals

The present invention is β of grains capable of efficiently separating and concentrating (1-3), (1-4) -β-D-glucan (hereinafter referred to as "β-glucan"), which are bioactive substances contained in various grains. It relates to a glucan extraction method.

Dietary fiber is a soluble and insoluble component of food which is not digested by enzymes in the gastrointestinal tract, and constitutes cell wall materials such as cellulose, hemicellulose, lignin and pectin in addition to gums and mucilage. It has also been demonstrated that dietary fiber prevents characteristic colon diseases, including colon cancer and diverticulitis, and promotes bowel movements to prevent constipation.

These dietary fibers are roughly divided into synthetic fiber and natural dietary fiber. Since synthetic fiber is not produced in Korea at present, it relies on total imports, and domestic peers are actively researching extraction of natural dietary fiber. .

Β-glucan, which is a kind of natural fiber in cereal grains, lowers blood cholesterol and LDL-cholesterol levels in the body, increases HDL-cholesterol levels, suppresses cholesterol and decreases digestion of fat. It is a very useful physiologically active substance that has been gaining worldwide attention as its efficacy is known. Such β-glucan is a component constituting the cell wall of the plant, and is known to be contained in grains such as barley, oats and rye. However, grains contain a small amount of β-glucan, and thus, in order to express the above-mentioned efficacy, there is a hassle of ingesting a large amount of grains having poor palatability.

Korean Application No. 91-13735 (Patent Publication No. 95-2867) proposed to solve this trouble discloses a method for producing water-soluble β-glucan from barley slope, the contents of which are as follows.

The contents of the method include a first step of separating the embryo portion of barley, a second step of inactivating β-glucanase by dipping the embryo-treated barley in ethanol, and solids by standing and centrifugation. A third step of separating, a fourth step of decomposing the protein with proteolytic enzyme solution, a fifth step of separating the aqueous β-glucan by precipitation and centrifugation, and a separate aqueous β-glucan A series of preparations by a sixth step of crystallization using ethanol, a seventh step of vacuum concentrating ethanol remaining in the crystallized β-glucan, and an eighth step of drying and pulverizing the concentrated agglomerate β-glucan The process mentioned above achieves the process.

However, this method is difficult to change the barley lean tissue to the extent that β-glucan can be easily extracted since (a) the second to fifth processes are carried out by the barley sill itself from which the embryo is removed, and the proteolytic efficiency The low yield of the finally produced β-glucan and (B) inactivation of β-glucanase and β-glucan crystallization are overlapped by ethanol, so that complex ethanol processing equipment must be separately installed. There is this.

Accordingly, the present invention has been made in consideration of the above problems, and an object thereof is to provide a method for extracting β-glucan of grains capable of separating and concentrating high yield of β-glucan contained in grains by a simple process.

The object of this invention is the 1st process of fine-grain-processing the grains containing (beta) -glucan, hydrolyzing into the powder, and extracting an aqueous solution; A second step of heating the extracted aqueous solution to inactivate β-glucanase and coagulating the protein, and then filtering and separating the solution except the coagulated material by adding and reacting starch degrading enzyme; It is achieved by the β-glucan extraction method of grains comprising a third step of separating the solid β-glucan by adding alcohol or alcohol to the filtered solution.

Hereinafter, the β-glucan extraction method of barley according to an embodiment of the present invention will be described as follows. The same purpose may be achieved even when applied to grains such as rye and oats.

<Step 1> Raw Material Processing

First of all, the barley or rice barley that has undergone the venous process is pulverized to have a particle size of 0.5 mm or less using a grinder to be powdered. At this time, since the particle size of the powder has a proportional relationship with the yield of β-glucan, it is preferable to finely crush barley.

<Step 2> Extraction Step

For the finely ground powder, 5-10 times the amount of hot water at 40-60 ° C. was added and mixed, and stirred for 1 to 2 hours, followed by standing or centrifugation at a low temperature of 2-4 ° C. to precipitate the precipitate. Remove and extract supernatant. This extraction process is usually repeated twice.

<Step 3> Heat Treatment and Enzyme Treatment

The extracted supernatant is heated to 60-100 ° C., preferably 60 ° C. for 10 minutes to inactivate β-glucanase and coagulate the protein. Thereafter, starch degrading enzymes, such as thermostable α-amylase, are added thereto, reacted with slow stirring for about 20 minutes, and the solution is recovered by removing coagulants using a method of removing cotton cloth or coagulants. .

<Step 4> Separation and concentration

The same amount of alcohol or alcohol is added to the filtered solution, and the mixture is stirred. After standing for 2 to 3 hours, the white substance (β-glucan) is coagulated and precipitated, and the supernatant is centrifuged or filtered. If removed by a method such as to separate the solid β-glucan.

<Step 5> Purification and Drying

5-10 times of water is added to the isolated β-glucan to dissolve, followed by precipitation of pure β-glucan by adding the same amount of alcohol or alcohol (supernatant = impurity + alcohol or alcohols), and then precipitated. [beta] -glucan is compressed by filtration using cotton cloth or the like, and is then dried by air drying or freeze-drying at room temperature to purify β-glucan. Then, finely pulverize the purified β-glucan mass according to the purpose of use.

By this process, about 3 to 4 g of β-glucan having a purity of 90 to 90% per 100 g of rice barley could be obtained.

As described above, the present invention has an effect of mass production of β-glucan having a high purity of about 80 to 90% by a simple process. In addition, when the present invention is used in the barley alcohol production process, there is an effect that can be produced inexpensive β-glucan at a low cost.

Claims (8)

a first step of finely treating the grains containing β-glucan, adding water to the powder, and extracting an aqueous solution; A second step of heating the extracted aqueous solution to inactivate β-glucanase and coagulating the protein, and then filtering and separating the solution except the coagulated material by adding and reacting starch degrading enzyme; Separation method of β-glucan of grains comprising a third step of separating the solid β-glucan by adding alcohol or alcohol to the filtered solution. According to claim 1, wherein the first step is stirred for 1 to 2 hours by adding 5 to 10 times of hot water of about 40 to 60 ℃ to the grain powder and then stirred at 2 to 4 ℃ or centrifugation Β-glucan separation method of grains, characterized in that the extraction of the supernatant twice after separation. The method of claim 1 or 2, wherein the grain powder of the first step is finely pulverized β-glucan separation method, characterized in that to have a particle size of 5mm or less. The method of claim 1, wherein the grains are β-glucan separation method of grains, such as cereals such as barley, rye, oats. The method of claim 1, wherein the second step is to heat the aqueous solution extracted in the first step to 60 ~ 100 ℃ to inactivate β-glucanase, coagulate the protein, and then add a thermostable α-amylase Β-glucan separation method of grains, characterized in that the cooling after the reaction. The method of claim 1, wherein the third step is stirred by adding the same amount of alcohol or alcohol to the solution separated in the second step and slowly stirred, and then the supernatant except for β-glucan solidified to stand for 2-3 hours The β-glucan separation method of grains, characterized in that to obtain a solid β-glucan by filtration or centrifugation. The method according to any one of claims 1 to 6, wherein the solid β-glucan is hydrolyzed and dissolved after the third step, and alcohol or alcohol is added to purify the precipitated pure β-glucan by compression filtration. 4 steps; Separating method of β-glucan of grains, characterized in that it further comprises a fifth step of powdering the compressed β- glucan solid. 8. The method of claim 7, wherein the fourth step is to dissolve the solid β-glucan of the third step by hydrolyzing 5-10 times the amount of water, adding alcohol or alcohols, and compressing and filtering the precipitated pure β-glucan. Separation method of β-glucan of cereals, characterized in that the purified solid β- glucan at room temperature air-dried or lyophilized.