JPS6415656A - Method for quantifying c reactive protein - Google Patents

Method for quantifying c reactive protein

Info

Publication number
JPS6415656A
JPS6415656A JP17168787A JP17168787A JPS6415656A JP S6415656 A JPS6415656 A JP S6415656A JP 17168787 A JP17168787 A JP 17168787A JP 17168787 A JP17168787 A JP 17168787A JP S6415656 A JPS6415656 A JP S6415656A
Authority
JP
Japan
Prior art keywords
reactive protein
absorbancy
specimen
change
fluocculation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP17168787A
Other languages
Japanese (ja)
Other versions
JP2534067B2 (en
Inventor
Takayuki Tanaka
Hideaki Shibata
Teruaki Hasegawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nissui Pharmacetuical Co Ltd
Original Assignee
Nissui Pharmacetuical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nissui Pharmacetuical Co Ltd filed Critical Nissui Pharmacetuical Co Ltd
Priority to JP62171687A priority Critical patent/JP2534067B2/en
Publication of JPS6415656A publication Critical patent/JPS6415656A/en
Application granted granted Critical
Publication of JP2534067B2 publication Critical patent/JP2534067B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

PURPOSE:To accurately quantity C reactive protein by utilizing a general-purpose biochemical automatic analyser, by adding an anti-C reactive protein IgG antibody to a specimen and measuring the change quantity of absorbancy dye to generated fluocculation. CONSTITUTION:A nonionic surfactant for shortening a measuring time due to the promotion of flocculation is used and an specimen is held to a definite pH range and the first reagent consisting of a buffer solution for preventing turbidity, a preservative and inorg. salt is added to the specimen serum and, next, the second reagent containing an anti-C reactive protein IgG antibody is added. Absorbancy is measured at every predetermined time and the change of absorbancy in a period when a fluocculation forming amount almost increase primarily is measured. Then, a calibration curve is used to quantity C reactive protein from the change amount of absorbancy and C reactive protein mass is calculated using a biochemical automatic analyser.
JP62171687A 1987-07-09 1987-07-09 Quantitative method for C-reactive protein Expired - Lifetime JP2534067B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62171687A JP2534067B2 (en) 1987-07-09 1987-07-09 Quantitative method for C-reactive protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62171687A JP2534067B2 (en) 1987-07-09 1987-07-09 Quantitative method for C-reactive protein

Publications (2)

Publication Number Publication Date
JPS6415656A true JPS6415656A (en) 1989-01-19
JP2534067B2 JP2534067B2 (en) 1996-09-11

Family

ID=15927830

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62171687A Expired - Lifetime JP2534067B2 (en) 1987-07-09 1987-07-09 Quantitative method for C-reactive protein

Country Status (1)

Country Link
JP (1) JP2534067B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5945063A (en) * 1997-02-25 1999-08-31 Tokyo Yogyo Kabushiki Kaisha Bottom blown gas blowing apparatus for molten metal ladle
US6623921B2 (en) 1998-07-30 2003-09-23 Advanced Life Science Institute, Inc. Method for measurement of hepatitis C virus

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58187862A (en) * 1982-04-27 1983-11-02 Sanyo Chem Ind Ltd Agent and method for improving immunological assay
JPS5992354A (en) * 1982-11-18 1984-05-28 Sekisui Chem Co Ltd Reagent for immune serological diagnosis
JPS59192962A (en) * 1983-04-15 1984-11-01 Kyowa Hakko Kogyo Co Ltd Latex reagent
JPS61243363A (en) * 1985-04-22 1986-10-29 Nitsusui Seiyaku Kk Highly sensitive assay of crp
JPS62498A (en) * 1985-06-27 1987-01-06 Nippon Bio Tesuto Kenkyusho:Kk Monoclonal antibody
JPS62192661A (en) * 1986-02-20 1987-08-24 Nitsusui Seiyaku Kk Method for measuring crp by immune nephelometry
JPS62218864A (en) * 1986-03-20 1987-09-26 Hitachi Chem Co Ltd Quantitative analysis of human c reactive protein

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58187862A (en) * 1982-04-27 1983-11-02 Sanyo Chem Ind Ltd Agent and method for improving immunological assay
JPS5992354A (en) * 1982-11-18 1984-05-28 Sekisui Chem Co Ltd Reagent for immune serological diagnosis
JPS59192962A (en) * 1983-04-15 1984-11-01 Kyowa Hakko Kogyo Co Ltd Latex reagent
JPS61243363A (en) * 1985-04-22 1986-10-29 Nitsusui Seiyaku Kk Highly sensitive assay of crp
JPS62498A (en) * 1985-06-27 1987-01-06 Nippon Bio Tesuto Kenkyusho:Kk Monoclonal antibody
JPS62192661A (en) * 1986-02-20 1987-08-24 Nitsusui Seiyaku Kk Method for measuring crp by immune nephelometry
JPS62218864A (en) * 1986-03-20 1987-09-26 Hitachi Chem Co Ltd Quantitative analysis of human c reactive protein

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5945063A (en) * 1997-02-25 1999-08-31 Tokyo Yogyo Kabushiki Kaisha Bottom blown gas blowing apparatus for molten metal ladle
US6623921B2 (en) 1998-07-30 2003-09-23 Advanced Life Science Institute, Inc. Method for measurement of hepatitis C virus

Also Published As

Publication number Publication date
JP2534067B2 (en) 1996-09-11

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