JPS60205335A - Sheath flow cell device - Google Patents

Sheath flow cell device

Info

Publication number
JPS60205335A
JPS60205335A JP6443184A JP6443184A JPS60205335A JP S60205335 A JPS60205335 A JP S60205335A JP 6443184 A JP6443184 A JP 6443184A JP 6443184 A JP6443184 A JP 6443184A JP S60205335 A JPS60205335 A JP S60205335A
Authority
JP
Japan
Prior art keywords
flow cell
light
main body
reflecting surface
photodetector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP6443184A
Other languages
Japanese (ja)
Inventor
Shiro Tsuji
史郎 辻
Kunihiko Okubo
邦彦 大久保
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Shimazu Seisakusho KK
Original Assignee
Shimadzu Corp
Shimazu Seisakusho KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp, Shimazu Seisakusho KK filed Critical Shimadzu Corp
Priority to JP6443184A priority Critical patent/JPS60205335A/en
Publication of JPS60205335A publication Critical patent/JPS60205335A/en
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • G01N15/1436Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

PURPOSE:To increase the ability in detection without using any large-output laser generator by providing a concave reflecting surface to the periphery of the main body of the sheath flow cell of a cell analyzer, etc., for measuring fluorescence, and converging reflected light on a photodetection part. CONSTITUTION:A sample is put in a flow chamber 1 from a pipe 4 and diluting solution is put in from a pipe 5; and the sample is flowed in the sheath of the dilute solution with the flow cell main body 2 and irradiated with exciting light to detect generated fluorescence. At this time, a half rotary elliptic block 6 is provided to the periphery of the flow cell main body 2. This block 6 has the concave reflecting surface and the photodetection part 3 of a photodetector is provided at its focal point. Further, the exciting light 16 is made incident from a window 11 and scattered light from a window 11 is received by the photodetection part 17. Therefore, light dispersed in every direction from the center of a measurement part is reflected by the concave reflecting surface and converged on the photodetection part 3, so the ability in detection is increased without using any large-output laser.

Description

【発明の詳細な説明】 (イ)産業上の利用分野 本発明は、けい光活性化された細胞をそのけい光により
識別するセルアナライザ、もしくは識別した細胞を分離
捕集するセルソータに組み込まれるシースフローセル装
置に関する。
Detailed Description of the Invention (a) Industrial Application Field The present invention relates to a sheath that is incorporated into a cell analyzer that identifies fluorescently activated cells by their fluorescence, or a cell sorter that separates and collects identified cells. This invention relates to a flow cell device.

(ロ)従来技術 セルアナライザ等のシースフローセルti、第1図に示
すように1測定すべき細胞を含む試料液と希釈液とをフ
ローチャンバー1に導入し、試料液を希釈液の°さや″
(シース)K包んだ状態で、細胞が一列に配列されるま
で細く絞り、これをフローセル本体2内を流下させ励起
光ビームBが照射される測定部に通すものであるが、従
来のセルアナライザ等の測定部で放射されるけい光は極
めて弱く、そのためけい光の検出限界が低かった。
(b) In a sheath flow cell such as a conventional cell analyzer, as shown in FIG.
(Sheath) Wrapped in K, the cells are narrowly squeezed until they are arranged in a line, and then flowed down inside the flow cell main body 2 and passed through the measurement section where the excitation light beam B is irradiated, but unlike the conventional cell analyzer The fluorescence emitted by the measurement unit was extremely weak, and therefore the detection limit of fluorescence was low.

この検出限界を上げようとすれば、励起光用のレーザー
発生装置の出力を大きくすればよいが、大出力のレーザ
ー発生装置は非常に高価でセルアナライザ等の装置全体
のコストが著しく増大するばかりでなく、水冷の必要性
があって装置全体の構造が大型化、複雑化する欠点があ
った。また従来のものは、けい光検出器の受光s3の前
方に集光用レンズを配しているだけであるので、四方に
放射される螢光の一部しか検出器に導くことができず、
この点でも検出限界の引き上げが困難だった。
If you want to increase this detection limit, you can increase the output of the excitation light laser generator, but a high-output laser generator is extremely expensive and will only significantly increase the cost of the entire device such as a cell analyzer. However, there was a drawback that water cooling was required, making the overall structure of the device larger and more complicated. In addition, the conventional type only has a condensing lens in front of the light receiving s3 of the fluorescence detector, so only a part of the fluorescence emitted in all directions can be guided to the detector.
This also made it difficult to raise the detection limit.

(ハ) 目的 本発明は、光検出器へのけい光の集光率を高め、大出力
のレーザー発生装置によらずにけい光検出限界を上げる
ことを目的としている。
(c) Purpose The present invention aims to increase the collection rate of fluorescence onto a photodetector and raise the detection limit of fluorescence without using a high-output laser generator.

に)構成 本発明は、励起光ビームが照射されるンースフローセル
の測定部の周囲に、側方の光検出器の側へ開いた凹入反
射面を形成して、該反射面により測定部内で放射された
けい光が反射され前記光検出器受光部に集まるようにし
たものである。
B) Structure The present invention forms a recessed reflective surface that opens toward the photodetector on the side around the measurement section of the flow cell to which the excitation light beam is irradiated, and the reflection surface allows the inside of the measurement section to be reflected. The fluorescent light emitted by the sensor is reflected and collected at the light receiving section of the photodetector.

(ホ)実施例 以下本発明の詳細を図示の実施例に基づいて説明する。(e) Examples The details of the present invention will be explained below based on the illustrated embodiments.

第2図及び第3図に示すのは本発明の第1の実施例であ
って、フローチャンバー1には試料液パイプ4と希釈液
パイプ5とが接続されている。フローセル本体2はフロ
ーチャンバー1の下面部から垂下しているが、励起光が
照射される測定部中心Mの近傍には、フローセル本体2
と同じく透光性材料(例えばガラス)から成る半裁の回
転楕円体形状のブロック6が一体に連成されている。こ
のブロック6の回転軸Rは、フローセル本体2内の通路
7と測定部中心Mにおいて直交しており、且つこの測定
部中心Mは回転楕円体の一方の焦点と一致している。こ
のブロック6の楕円外周面に鏡面コート層8を設けるこ
とによって、測定部中心Mの周囲に、側方へ開いた凹入
反射面9が形成されている。10は保護層である。ブロ
ック6の半裁側の端面6aは、透明な平面、もしくはレ
ンズ状の曲面とする。尚、凹入反射面9には窓孔11.
12を設けて、励起光ビーム及びその散乱光がそれぞれ
透過するようにしである。光検出器の受光部3は、はぼ
回転楕円体の第2焦点Q上に配置する。
2 and 3 show a first embodiment of the present invention, in which a flow chamber 1 is connected to a sample liquid pipe 4 and a diluent pipe 5. In FIG. The flow cell main body 2 hangs down from the lower surface of the flow chamber 1, but the flow cell main body 2 is located near the center M of the measurement section where the excitation light is irradiated.
A half-shaped spheroid-shaped block 6 made of a transparent material (for example, glass) is coupled together. The rotation axis R of this block 6 is orthogonal to the passage 7 in the flow cell main body 2 at the measurement part center M, and the measurement part center M coincides with one focal point of the spheroid. By providing a specular coating layer 8 on the elliptical outer circumferential surface of this block 6, a concave reflective surface 9 that opens laterally is formed around the center M of the measuring section. 10 is a protective layer. The end surface 6a of the block 6 on the half-cut side is a transparent plane or a lens-shaped curved surface. Note that the recessed reflective surface 9 has a window hole 11.
12 is provided so that the excitation light beam and its scattered light are transmitted respectively. The light receiving section 3 of the photodetector is placed on the second focal point Q of the spheroid.

第2図中、16は励起光の照射部、17V!散乱光の受
光部、18は透過光のストソ・ζである。
In Figure 2, 16 is the excitation light irradiation part, 17V! A light receiving section 18 for scattered light is a light receiving section 18 for transmitting light.

第3図に示すのは本発明の第2実施例であって、凹面鏡
13により凹入反射面9′を構成したものである。この
凹入反射面9′の形状は第1図の例における反射面9と
同じように回転楕円面形状で、その回転軸Rがフローセ
ル本体2の通路7と測定部中心Mにおいて直交し、且つ
測定部中心Mが楕円の一方の焦点と一致している。14
は凹面鏡13の開1]部に取り付けたレンズ、15は凹
面鏡13の固定部材である。この例においても、反射面
9′に励起光及びその散乱光が透過する窓孔11’、1
2′が形成される。
FIG. 3 shows a second embodiment of the present invention, in which a concave mirror 13 constitutes a concave reflecting surface 9'. The shape of this recessed reflecting surface 9' is a spheroidal shape similar to the reflecting surface 9 in the example of FIG. The center M of the measurement part coincides with one focus of the ellipse. 14
1 is a lens attached to the opening 1 of the concave mirror 13, and 15 is a fixing member of the concave mirror 13. In this example as well, window holes 11' and 1 through which excitation light and its scattered light pass through the reflecting surface 9'.
2' is formed.

尚、光検出器受光部は一定の受光面積を有しているから
、反射光はこの面積内に入ればよく、従って必ずしも反
射光が一点に集中するよう、凹入反射面9,9′の形状
を回転楕円面に限定する必要はなく、回転楕円面に類似
した形状であって、測定部中心Mからの放射光を反射に
より側方の光検出器受光部の受光面積内に集光できるも
のであればよい。反射面9,9′による反射光が光検出
器受光部の近傍で広く拡散している場合は、その前方に
集光レンズを介在させればよく、ブロック6により反射
面9を形成したものでは、ブロックの端面をレンズ状曲
面とし、凹面鏡13で反射面9′を形成したものでは、
凹面鏡13開口部処レンズを設ける。
Note that since the light receiving section of the photodetector has a certain light receiving area, the reflected light only needs to fall within this area. It is not necessary to limit the shape to an ellipsoid of revolution, but the shape is similar to an ellipsoid of revolution, and the light emitted from the center M of the measurement part can be focused within the light receiving area of the light receiving part of the photodetector on the side by reflection. It is fine as long as it is something. If the light reflected by the reflective surfaces 9 and 9' is widely diffused near the light receiving section of the photodetector, a condensing lens may be inserted in front of the light receiving section of the photodetector. , in which the end face of the block is a lens-like curved surface and the reflective surface 9' is formed by a concave mirror 13,
A concave mirror 13 is provided with an aperture treatment lens.

(へ)効果 本発明によれば、測定部中心から四方に放射された光は
、凹入反射面に反射されて光検出器の受光部に集まるか
ら、受光部に入る光景が大となり、大出力のレーザー発
生装置を用いなくとも、光の検出限界を高めることがで
き、低出力の励起用光源ヲ用いてセルアナライザ等の装
置全体を小型化するとともに、より載量単位の検出をす
ることができる。
(f) Effects According to the present invention, the light emitted in all directions from the center of the measuring section is reflected by the recessed reflective surface and collected at the light receiving section of the photodetector, so the sight that enters the light receiving section becomes large. It is possible to increase the detection limit of light without using a high-power laser generator, and by using a low-power excitation light source, the entire device such as a cell analyzer can be miniaturized, and it is possible to detect more loading units. Can be done.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図は従来のシースフロー七ル装置の概略斜視図、第
2図は本発明の一実施例の概略斜視図、第3図は同上実
施例の縦断面図、第4図は他の実施例の縦断面図である
。 2・・フローセル本体、3・・光検出器受光部、6・・
ブロック、7・・通路、9,9′・・凹入反射面、13
・・凹面鏡。 出 願 人 株式会社島津製作所 代 理 人 弁理士岡田和秀 第3図 第4図
Fig. 1 is a schematic perspective view of a conventional sheath flow device, Fig. 2 is a schematic perspective view of an embodiment of the present invention, Fig. 3 is a longitudinal sectional view of the same embodiment, and Fig. 4 is another embodiment. FIG. 3 is an example vertical cross-sectional view. 2. Flow cell body, 3. Photodetector light receiving section, 6.
Block, 7... Passage, 9, 9'... Recessed reflective surface, 13
··concave mirror. Applicant: Shimadzu Corporation Agent: Patent Attorney Kazuhide Okada Figure 3 Figure 4

Claims (1)

【特許請求の範囲】[Claims] (1)励起光ビームが照射されるフローセル本体の測定
部の周囲に、該測定部側方の光検出器の側に開いた凹入
反射面を形成して、測定部中心からの放射光を光検出器
受光部へ反射集光するよ’)Kしたシースフローセル装
置。
(1) A recessed reflective surface that opens toward the photodetector on the side of the measurement section is formed around the measurement section of the flow cell body to which the excitation light beam is irradiated, so that the emitted light from the center of the measurement section is reflected. A sheath flow cell device that reflects and focuses light onto the light receiving part of the photodetector.
JP6443184A 1984-03-30 1984-03-30 Sheath flow cell device Pending JPS60205335A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6443184A JPS60205335A (en) 1984-03-30 1984-03-30 Sheath flow cell device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6443184A JPS60205335A (en) 1984-03-30 1984-03-30 Sheath flow cell device

Publications (1)

Publication Number Publication Date
JPS60205335A true JPS60205335A (en) 1985-10-16

Family

ID=13258075

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6443184A Pending JPS60205335A (en) 1984-03-30 1984-03-30 Sheath flow cell device

Country Status (1)

Country Link
JP (1) JPS60205335A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62285043A (en) * 1986-06-03 1987-12-10 Omron Tateisi Electronics Co Flowcell with condenser
JP2014112108A (en) * 1997-01-31 2014-06-19 Xy Llc Optical apparatus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62285043A (en) * 1986-06-03 1987-12-10 Omron Tateisi Electronics Co Flowcell with condenser
JP2014112108A (en) * 1997-01-31 2014-06-19 Xy Llc Optical apparatus

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