JPS5968674A - Clinical inspection means - Google Patents
Clinical inspection meansInfo
- Publication number
- JPS5968674A JPS5968674A JP17961182A JP17961182A JPS5968674A JP S5968674 A JPS5968674 A JP S5968674A JP 17961182 A JP17961182 A JP 17961182A JP 17961182 A JP17961182 A JP 17961182A JP S5968674 A JPS5968674 A JP S5968674A
- Authority
- JP
- Japan
- Prior art keywords
- thickness
- plate
- test
- silicon oxide
- oxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、免疫反応を用いる検査に使用される臨床検査
具に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a clinical test tool used for tests using immune reactions.
免疫反応を用いる検査としては、例えば赤血球凝集反応
、ラテックス凝集反応、カーボン凝集反応、シリカ凝集
反応等の凝集反応を用いる凝集判定検査、放射性同位元
素で標識した標識抗原と、非標識抗原とに対する抗体の
競争反応を利用する放射免疫測定検査、酵素で標識され
た抗原（又は抗体）と抗体（又は抗原）との結合体の酵
素活性を測定する酵素免疫測定検査、ヒト組織適合性、
遺伝子座抗原と抗血清との反応によるＨＬＡ検査等多種
類のものが広汎に用いられている。Tests that use immune reactions include, for example, agglutination tests that use agglutination reactions such as red blood cell agglutination, latex agglutination, carbon agglutination, and silica agglutination, and antibodies against labeled antigens labeled with radioactive isotopes and unlabeled antigens. radioimmunoassay test that utilizes the competitive reaction of
Many types of tests, such as HLA tests based on the reaction between gene locus antigens and antiserum, are widely used.
これらの検査においては、免疫反応の場を与えるだめの
各種の検査具が存在する。In these tests, there are various test tools that provide a venue for immune reactions.
これらの検査具の形状は様々であり、プレート状、チュ
ーブ状、多数個の穴部を有する形状等のものがある。These test tools have various shapes, such as a plate shape, a tube shape, and a shape having multiple holes.
従来、これらの検査具としてガラス製のものが使用され
て米たが、近年破損し離＜、成形加工の容易な合成樹脂
製のものが好んで使用されるに至っている。Conventionally, test tools made of glass have been used, but in recent years, test tools made of synthetic resin have come to be preferred because of their breakage and easy molding process.
しかしながら、従来の合成樹脂製の検査具の有する欠点
は、それらの表面が疎水性であシ、血清、検査試薬液、
水等に濡れ難い点及び血清中の蛋白質が無声側に吸着し
易く、免疫反応に於ける非特異反応が生じ易い点に存し
ていた。However, the disadvantage of conventional synthetic resin test tools is that their surfaces are hydrophobic, which means that they cannot be used for serum, test reagents, etc.
The problem lies in the fact that it is difficult to wet with water, etc., and proteins in serum tend to be adsorbed to the silent side, which tends to cause non-specific reactions in immune reactions.
本発明者らは、合成樹脂材質の長所を失なわせずに且つ
、上記の欠点を解消するために鋭意検討を進めた結果、
これらの合成樹脂製検査具の表面に金属酸化物の薄４を
設ける事が極めて有効であることを見出し、本発明を完
成するに至った。The inventors of the present invention have carried out extensive studies in order to eliminate the above-mentioned drawbacks without losing the advantages of synthetic resin materials.
We have found that it is extremely effective to provide a thin layer of metal oxide 4 on the surface of these synthetic resin test tools, and have completed the present invention.
本発明の要旨は、合成樹脂基材の表面に、金属酸化物の
薄層が形成されていることを特徴とする、臨床検査具に
存する。The gist of the present invention resides in a clinical testing device characterized in that a thin layer of metal oxide is formed on the surface of a synthetic resin base material.
次に本発明臨床検査具について更に詳細に説明する。Next, the clinical testing device of the present invention will be explained in more detail.
本発明を構成する合成樹脂としては、例えばポリスチレ
ン、ポリメチルメタクリレート、ポリ塩化ビニル、ポリ
カーボネート、アクリロニトリル−メチルアクリレート
共重合体、スチレン−無水マレイン酸共重合体、スチレ
ン−アクリ０ ＝ ） ！ｊ ル共ｍ合体、アセチルセ
ルロース、アセチルブチルセルロース、アセタール化ポ
リビニルアルコール、ポリプロピレン、エチレン−プロ
ピレン共重合体、ポリエステル、ポリメチルペンテン−
１等を挙けることができるが、特に好ましくは、処理効
果が特に顕著であるポリスチレン、スチレン−アクリロ
ニトリル共重合体、スチレン−無水マレイン酸共重合体
、及びポリ塩化ビニルである。これらの合成樹脂中には
、充填剤、着色剤、安定剤等が含有されていてもよい。Examples of the synthetic resins constituting the present invention include polystyrene, polymethyl methacrylate, polyvinyl chloride, polycarbonate, acrylonitrile-methyl acrylate copolymer, styrene-maleic anhydride copolymer, and styrene-acrylic 0 = )! j Le co-m combination, acetyl cellulose, acetyl butyl cellulose, acetalized polyvinyl alcohol, polypropylene, ethylene-propylene copolymer, polyester, polymethylpentene
Among them, particularly preferred are polystyrene, styrene-acrylonitrile copolymer, styrene-maleic anhydride copolymer, and polyvinyl chloride, which have particularly remarkable treatment effects. These synthetic resins may contain fillers, colorants, stabilizers, and the like.
本発明検査具の表面に形成される金属酸化物としては例
えば、酸化ケイ素、酸化チタン、酸化アルミニューム、
酸化インジューム、スズ酸化物、メンタル酸化物、ベリ
リューム酸化物、ケイ素酸化物とホウ素酸化物との複合
物等を挙けることができる。Examples of metal oxides formed on the surface of the test tool of the present invention include silicon oxide, titanium oxide, aluminum oxide,
Examples include indium oxide, tin oxide, mental oxide, beryllium oxide, and a composite of silicon oxide and boron oxide.
これらのものの内、特に好ましいものは、酸化ケイ素、
及びケイ素酸化物とホウ素酸化物との複合物である。Among these, particularly preferred are silicon oxide,
and a composite of silicon oxide and boron oxide.
該金属酸化物の薄層を形成させる手段としては、真空蒸
着法、イオンブレーティング法、グロー放電法、金属蒸
気に酸素を反応させ乍ら薄膜を形成せしめる反応蒸着法
等が採られる。Examples of means for forming the thin layer of the metal oxide include vacuum evaporation, ion blating, glow discharge, and reactive evaporation in which metal vapor is reacted with oxygen to form a thin film.
これらの方法の内、特に好適に用いられるのは真空蒸着
法であり、この場合には１×１０ないしｌＸ１０ 削
１１ｇレベルの高真空下で上記の酸化物を蒸発させ乍ら
、検査器材の表面にこれを蒸着せしめる。Among these methods, the vacuum evaporation method is particularly preferably used. In this case, the above-mentioned oxide is evaporated under a high vacuum of 1 x 10 to 1 x 10 g, and the surface of the inspection equipment is This is vapor-deposited on the surface.
該金属酸化物の薄層の厚みとしては、５０乃至３０００
オングストロームの範囲にあることが好ましい。該薄層
の厚みが５０オングストロームよりも小さい場合には、
合成樹脂製検査器材表面の親水化あるいは、蛋白分子の
吸着性抑制の効果が発現され難い。逆に該薄層の厚みが
３０００オングストロームよシも大きい場合には、親水
化効果は充分であるが、薄層が破壊され易く、検査器材
表面で安定的に保持され難くなる。The thickness of the thin layer of metal oxide is 50 to 3000 mm.
Preferably, it is in the angstrom range. If the thickness of the thin layer is less than 50 angstroms,
It is difficult to make the surface of synthetic resin test equipment hydrophilic or to suppress the adsorption of protein molecules. On the other hand, if the thickness of the thin layer is as large as 3000 angstroms, the hydrophilic effect is sufficient, but the thin layer is easily destroyed and difficult to be stably held on the surface of the test instrument.
本発明臨床検査具を製作する方法としては、予じめ、シ
ート、チューブ、有底のチューブ、複雑形状を有する異
型成型体等に成型されたものの表面に、上記の方法で薄
膜を形成させることができる。別の方法として、シート
状成型物に該薄膜を形成させた後、これを加工して、目
的に応じた形状になすことも有効な方法である。The method for manufacturing the clinical testing device of the present invention is to form a thin film using the method described above on the surface of a sheet, tube, bottomed tube, irregular molded body having a complicated shape, etc. I can do it. Another effective method is to form the thin film on a sheet-like molded product and then process the thin film into a shape suitable for the purpose.
上記の様にして得られる臨床検査具は、その表面がガラ
ス質の性状を有し、親水性に富む許υでなく、不必要な
蛋白質の吸着を抑える性質を有している。このため、本
発明臨床検査具は、例えば、ラテックス凝集判定用プレ
ート、赤血球凝集判定用マイクロタイターグレート、Ｈ
ＬＡ検査検査用テラツキプレート射免疫測定用チューブ
、酵素免疫測定用チューブ、免疫電気泳動用プレート等
として極めて好適である。The clinical test device obtained as described above has a glassy surface, is not highly hydrophilic, and has the property of suppressing the adsorption of unnecessary proteins. Therefore, the clinical test device of the present invention includes, for example, a plate for latex agglutination determination, a microtiter grate for red blood cell agglutination determination, and H
The Teratsuki plate for LA testing is extremely suitable as a tube for immunoassay, a tube for enzyme immunoassay, a plate for immunoelectrophoresis, etc.
実施例１
厚さ２００ノの透明なポリエチレンテレフタレ×１０
鰭−の真空下で蒸発させ乍ら、薄膜を形成せしめた。得
られた蒸着層の厚みは１５００オングストロームの厚さ
を有していた。Example 1 200 mm thick transparent polyethylene terephthalate x 10
A thin film was formed while evaporating under fin vacuum. The resulting deposited layer had a thickness of 1500 angstroms.
この蒸着フィルムを用いて、リウマチ因子検出のための
ＲＡテストをＲＡキットを用いて実施した。グリシン食
塩水緩衝液１．Ｏｐｌ を小試験管にとシ、これに陰
性及び陽性血清台１滴（０、０５ｍｅ ）を滴下し、よ
く混合した。次いで臨尿検査用プレートの別々の区画に
これらの希釈血清を、夫々１滴（１０声ｅ）滴下した。Using this vapor-deposited film, an RA test for detecting rheumatoid factor was carried out using an RA kit. Glycine saline buffer 1. Opl was placed in a small test tube, and 1 drop (0.05 me) of negative and positive serum was added thereto and mixed well. One drop (10 drops) of each of these diluted sera was then placed in separate compartments of the urine test plate.
更にヒト変性Ｔ−グロブリンを吸着させたポリスチレン
ラテックスからなる試薬１滴（１０声ｅ）を夫々の区画
に加えた後、血清とよく混和し、直径２５％の円形に液
滴を拡げた。Furthermore, one drop (10 volumes) of a reagent made of polystyrene latex adsorbed with human denatured T-globulin was added to each compartment, mixed well with serum, and the droplet was spread into a circle with a diameter of 25%.
この臨床検査用プレートを両手で持ち上げ、１分間程ゆ
るやかに動かした後、放置し肉眼で判定した。This clinical test plate was lifted with both hands, moved gently for about 1 minute, and then left to stand for evaluation with the naked eye.
この臨床検査用プレートにおいては、液滴の拡がり性が
良好で、陽性血清における凝集塊の現われ方が速やかで
あり、かつ明確な凝集像が得られ、ラテックス凝集判定
用プレートとして、好適なものであった。This plate for clinical testing has good droplet spreadability, the appearance of aggregates in positive serum quickly, and a clear agglutination image, making it suitable as a plate for latex agglutination determination. there were.
実施例２
厚さ１５０戸の透明なポリエチＶンテレフタレえながら
、５ｉ０２及びＢ２０．を電子ビーム加熱させて蒸発さ
せ蒸着層を形成せしめた。得られた蒸着層の厚みは８０
０オングストロームの厚さを有していた。この蒸着薄膜
層における５ｉ０２ とＢ２Ｏ３の比率は１対０．６で
あった。Example 2 5i02 and B20. was evaporated by electron beam heating to form a deposited layer. The thickness of the obtained vapor deposited layer was 80
It had a thickness of 0 angstroms. The ratio of 5i02 to B2O3 in this deposited thin film layer was 1:0.6.
この蒸着フィルムを用いて真空成型により、直径７％、
深さ２０％の孔４部を多数有する異型成型プレートを得
た。By vacuum forming using this vapor-deposited film, the diameter is 7%,
A irregularly molded plate having four holes each having a depth of 20% was obtained.
このようにして得た検査グレートを用いてＲ−ＰＨＡ法
による）ＩＢＳ抗原検査を実施した。Using the test grade thus obtained, an IBS antigen test (based on the R-PHA method) was conducted.
該検査プレートの合孔にドロッパーを用いてＲ−ＰＨＡ
緩衝液を０．０５ ｐｉづつ分法した。Using a dropper, add R-PHA to the matching hole of the inspection plate.
The buffer was aliquoted into 0.05 pi portions.
次いで、被検血清を０．０２５ ｐｉをダイリュータ−
を用いて取り出し、４段階に稀釈して加えた。Next, 0.025 pi of the test serum was diluted with
The solution was taken out and diluted in 4 steps and added.
ＨＢＳ抗原陽性対照として標準ＨＢＳ抗原を同様に稀釈
し並置した。その後プレートをミキサー上に置へ振盪さ
せ乍らｏ、 ６チＲ−ＰＨＡセル浮遊液０．０２５ ｐ
ｌを全ての孔に滴下した。A standard HBS antigen was similarly diluted and juxtaposed as an HBS antigen positive control. The plate was then placed on a mixer and shaken while adding 0.025p of 6T R-PHA cell suspension
1 was dropped into all holes.
このプレートを室温で２ないし３時間静置した後孔底の
赤血球凝集像を観察しだ。After this plate was allowed to stand at room temperature for 2 to 3 hours, the image of red blood cell agglutination at the bottom of the pores was observed.
比較のために該蒸着層を設けていないポリエチレンテレ
フタレートシートにより作成したプレートを用いて上記
試験を実施した。For comparison, the above test was conducted using a plate made of a polyethylene terephthalate sheet without the vapor deposited layer.
その結果、本発明金属酸化物蒸着層においてはプラス血
清とマイナス血清との赤血球凝集像の区分が極めて明確
に現われたのに対して、比較例プレートではその区分が
不明瞭でありこの目的の検査プレートとして不適なもの
であった。As a result, in the metal oxide vapor-deposited layer of the present invention, the division of red blood cell agglutination images between positive serum and negative serum appeared very clearly, whereas in the comparative plate, the division was unclear and was not suitable for this purpose test. It was unsuitable as a plate.
特；′１”出願人 「Ｌ′１水山パ、：土朶抹式会ネ１ 代表ｙｆ ｊ佇沼ノ、１．−利Special; '1' applicant ``L'1 Mizuyama Pa: Tohomashikikai Ne1 Representative yf j Tamano, 1. - profit
Claims (1)
されていることを特徴とする、臨床検査具 ２ 金属酸化物の５専層が５０乃至３０００オングスト
ロームの厚みを有するものである、特許請求の範囲第１
項記載の臨床検査具[Claims] 1. Clinical testing device 2, characterized in that a thin layer of metal oxide is formed on the surface of a synthetic resin base material. Claim 1, which has a thickness
Clinical test equipment described in section
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17961182A JPS5968674A (en) | 1982-10-13 | 1982-10-13 | Clinical inspection means |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17961182A JPS5968674A (en) | 1982-10-13 | 1982-10-13 | Clinical inspection means |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5968674A true JPS5968674A (en) | 1984-04-18 |
| JPH0153747B2 JPH0153747B2 (en) | 1989-11-15 |
Family
ID=16068774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17961182A Granted JPS5968674A (en) | 1982-10-13 | 1982-10-13 | Clinical inspection means |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5968674A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991005257A1 (en) * | 1989-10-02 | 1991-04-18 | Teijin Limited | Kit for immunoassay of human tissue plasminogen activator/human plasminogen activator inhibitor complex and method of immunoassay |
| JP2002086382A (en) * | 2000-07-14 | 2002-03-26 | Abb Ab | Manipulator |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5198320A (en) * | 1974-04-01 | 1976-08-30 | ||
| JPS5396317A (en) * | 1976-12-20 | 1978-08-23 | Gen Electric | Diagnostic tool and production thereof |
| JPS5396318A (en) * | 1976-12-23 | 1978-08-23 | Gen Electric | Diagnostic apparatus |
-
1982
- 1982-10-13 JP JP17961182A patent/JPS5968674A/en active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5198320A (en) * | 1974-04-01 | 1976-08-30 | ||
| JPS5396317A (en) * | 1976-12-20 | 1978-08-23 | Gen Electric | Diagnostic tool and production thereof |
| JPS5396318A (en) * | 1976-12-23 | 1978-08-23 | Gen Electric | Diagnostic apparatus |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991005257A1 (en) * | 1989-10-02 | 1991-04-18 | Teijin Limited | Kit for immunoassay of human tissue plasminogen activator/human plasminogen activator inhibitor complex and method of immunoassay |
| JP2002086382A (en) * | 2000-07-14 | 2002-03-26 | Abb Ab | Manipulator |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0153747B2 (en) | 1989-11-15 |
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