JPS5780398A - Plasmid produced by genetic manipulation, coliform bacillus having the same and preparation of tryptophan with said bacillus - Google Patents

Plasmid produced by genetic manipulation, coliform bacillus having the same and preparation of tryptophan with said bacillus

Info

Publication number
JPS5780398A
JPS5780398A JP55154706A JP15470680A JPS5780398A JP S5780398 A JPS5780398 A JP S5780398A JP 55154706 A JP55154706 A JP 55154706A JP 15470680 A JP15470680 A JP 15470680A JP S5780398 A JPS5780398 A JP S5780398A
Authority
JP
Japan
Prior art keywords
tryptophan
bacillus
strain
coliform
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55154706A
Other languages
Japanese (ja)
Other versions
JPH0226955B2 (en
Inventor
Shuichi Aiba
Tadayuki Imanaka
Hiroshi Tsunekawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SANRAKU OCEAN KK
Sanraku Inc
Sanraku Ocean Co Ltd
Original Assignee
SANRAKU OCEAN KK
Sanraku Inc
Sanraku Ocean Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SANRAKU OCEAN KK, Sanraku Inc, Sanraku Ocean Co Ltd filed Critical SANRAKU OCEAN KK
Priority to JP55154706A priority Critical patent/JPS5780398A/en
Priority to AU74644/81A priority patent/AU549686B2/en
Priority to CA000386858A priority patent/CA1182409A/en
Publication of JPS5780398A publication Critical patent/JPS5780398A/en
Publication of JPH0226955B2 publication Critical patent/JPH0226955B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

PURPOSE: To prepare tryptophan with a high productivity, by using coliform bacilli having an incorporated plasmid having a tryptophan operon released from the feedback inhibition by the tryptophan.
CONSTITUTION: Escherichia coil W3110 trp AE1 which is a strain of coliform bacilli derived from a strain of Escherichia coil K-12 subjected to the variant treatment, e.g. UV irradiation, and bacilli resistant to tryptophan among them are selected to give a strain in which the A Sase activity will not be inhibited by the tryptophan. The strain is then extracted to afford a recombined plasmid having a tryptophan operon released from the feedback inhibition by the tryptophan. Coliform bacilli in which the feedback inhibition is released by the introduction of the plasmid and the tryptophan repressor and the tryptophanase are deficient are cultivated in a culture medium, particularly containing anthranilic acid, and the tryptophan is then collected from the resultant culture.
COPYRIGHT: (C)1982,JPO&Japio
JP55154706A 1980-11-05 1980-11-05 Plasmid produced by genetic manipulation, coliform bacillus having the same and preparation of tryptophan with said bacillus Granted JPS5780398A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP55154706A JPS5780398A (en) 1980-11-05 1980-11-05 Plasmid produced by genetic manipulation, coliform bacillus having the same and preparation of tryptophan with said bacillus
AU74644/81A AU549686B2 (en) 1980-11-05 1981-08-26 Plasmid constructed by gene manipulation strains of e.coli to produce tryptophan
CA000386858A CA1182409A (en) 1980-11-05 1981-09-29 Plasmids constructed by gene manipulation, strains of escherichia coli carrying them, and process of tryptophan production using said strains

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55154706A JPS5780398A (en) 1980-11-05 1980-11-05 Plasmid produced by genetic manipulation, coliform bacillus having the same and preparation of tryptophan with said bacillus

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP63085130A Division JPS63273488A (en) 1988-04-08 1988-04-08 Production of tryptophan

Publications (2)

Publication Number Publication Date
JPS5780398A true JPS5780398A (en) 1982-05-19
JPH0226955B2 JPH0226955B2 (en) 1990-06-13

Family

ID=15590169

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55154706A Granted JPS5780398A (en) 1980-11-05 1980-11-05 Plasmid produced by genetic manipulation, coliform bacillus having the same and preparation of tryptophan with said bacillus

Country Status (3)

Country Link
JP (1) JPS5780398A (en)
AU (1) AU549686B2 (en)
CA (1) CA1182409A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS602189A (en) * 1983-06-17 1985-01-08 Mitsubishi Petrochem Co Ltd Novel plasmid
JPS6043392A (en) * 1983-08-20 1985-03-07 Showa Denko Kk Preparation of l-tryptophan
JPS61247392A (en) * 1985-02-04 1986-11-04 エンジニツクス　インコ−ポレ−テツド Recombined microorganism capable of simultaneously producingtwo or more amino acids
JP2016515400A (en) * 2013-04-16 2016-05-30 シージェイ チェイルジェダン コーポレーションＣｊ Ｃｈｅｉｌｊｅｄａｎｇ Ｃｏｒｐｏｒａｔｉｏｎ Microorganism having L-tryptophan production ability and method for producing L-tryptophan using the same

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
EP0293207A3 (en) * 1987-05-29 1989-11-02 The Standard Oil Company Eschericia coli carrying recombinant plasmid for the production of tryptophan
JPH0722510B2 (en) * 1988-05-09 1995-03-15 三井東圧化学株式会社 Gene encoding thermostable tryptophan synthase and its use
DE4232468A1 (en) 1992-09-28 1994-03-31 Consortium Elektrochem Ind Microorganisms for the production of tryptophan and process for their production
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
DE102005019040A1 (en) 2005-04-23 2006-10-26 Degussa Ag Process for the preparation of L-amino acids using improved strains of the family Enterobacteriaceae
DE102007051024A1 (en) 2007-03-05 2008-09-11 Evonik Degussa Gmbh Process for the preparation of L-amino acids using strains of the family Enterobacteriaceae
EP1975241A1 (en) 2007-03-29 2008-10-01 Evonik Degussa GmbH Method for manufacturing L-amino acids using improved strains of the enterobacteriaceae family
DE102007044134A1 (en) 2007-09-15 2009-03-19 Evonik Degussa Gmbh Process for the preparation of L-amino acids using improved strains of the family Enterobacteriaceae
DE102007052270A1 (en) 2007-11-02 2009-05-07 Evonik Degussa Gmbh Process for the preparation of L-amino acids using improved strains of the family Enterobacteriaceae
EP2060636A1 (en) 2007-11-14 2009-05-20 Evonik Degussa GmbH Method for manufacturing L-amino acids using improved strains of the enterobacteriaceae family
DE102008040352A1 (en) 2008-07-11 2010-01-14 Evonik Degussa Gmbh Process for the preparation of L-tryptophan using improved strains of the family Enterobacteriaceae
DE102008044768A1 (en) 2008-08-28 2010-03-04 Evonik Degussa Gmbh Process for the preparation of organochemical compounds using improved strains of the family Enterobacteriaceae
EP2628792A1 (en) 2012-02-17 2013-08-21 Evonik Industries AG Cell with reduced ppGppase activity
EP2762571A1 (en) 2013-01-30 2014-08-06 Evonik Industries AG Microorganism and method for the production of amino acids by fermentation
EP3385275B1 (en) 2017-04-07 2019-10-23 Evonik Degussa GmbH Method for producing aromatic l-amino acids using improved strains of the enterobacteriaceae family
US11053526B2 (en) 2018-08-09 2021-07-06 Evonik Operations Gmbh Process for preparing L amino acids using improved strains of the enterobacteriaceae family

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5771397A (en) * 1980-08-22 1982-05-04 Ajinomoto Co Inc Preparation of l-tryptophan by fermentation method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5771397A (en) * 1980-08-22 1982-05-04 Ajinomoto Co Inc Preparation of l-tryptophan by fermentation method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS602189A (en) * 1983-06-17 1985-01-08 Mitsubishi Petrochem Co Ltd Novel plasmid
JPS6043392A (en) * 1983-08-20 1985-03-07 Showa Denko Kk Preparation of l-tryptophan
JPS6348519B2 (en) * 1983-08-20 1988-09-29 Showa Denko Kk
JPS61247392A (en) * 1985-02-04 1986-11-04 エンジニツクス　インコ−ポレ−テツド Recombined microorganism capable of simultaneously producingtwo or more amino acids
JP2016515400A (en) * 2013-04-16 2016-05-30 シージェイ チェイルジェダン コーポレーションＣｊ Ｃｈｅｉｌｊｅｄａｎｇ Ｃｏｒｐｏｒａｔｉｏｎ Microorganism having L-tryptophan production ability and method for producing L-tryptophan using the same
US9562246B2 (en) 2013-04-16 2017-02-07 Cj Cheiljedang Corporation Microorganisms having L-tryptophan productivity and a method for production of L-tryptophan using same

Also Published As

Publication number Publication date
JPH0226955B2 (en) 1990-06-13
AU7464481A (en) 1982-05-13
AU549686B2 (en) 1986-02-06
CA1182409A (en) 1985-02-12

Similar Documents

Publication Publication Date Title
JPS5780398A (en) Plasmid produced by genetic manipulation, coliform bacillus having the same and preparation of tryptophan with said bacillus
JPS56144092A (en) Preparation of l-methionine by fermentation
EP0643135A4 (en) Process for producing l-threonine by fermentation.
GR3021105T3 (en) Production of proteins using homologous recombination
IL91826A (en) Human growth hormone analog
DK170186A (en) BIOLOGICALLY CULTURAL CULTURE OF A TREASURE LACTOBACILLUS ACIDOPHILUS AND PROCEDURE FOR INSULATING SUCH CULTURE AND APPLICATION OF THIS SUBSTANCE MEDICINE
SE8603145L (en) PROCEDURE FOR THE PREPARATION OF INTERFERON-BETA
GEP20002145B (en) Stem Cell Factor
BG49717A3 (en) Method for preparing of chirudine
Protić et al. Enhancement of DNA repair capacity of mammalian cells by carcinogen treatment
ES482097A1 (en) Novel acyl-derivatives of carnitine and process for their preparation
JPS56160997A (en) Preparation of l-lysine by fermenaition method
GR3022321T3 (en) Use of il-4 to treat solid tumors
JPS55156591A (en) Method for stabilizing property of bacteria containing plasmid
JPS5444096A (en) Preparation of l-arginine by fermentation
ES8201943A1 (en) 9-Aminoalkylfluorenes, process therefor and antiarrhythmic formulations thereof.
JPS5639778A (en) Novel modified strain
IL79432A (en) Unicellular microorganisms having invasive capability due to exogenous inv gene and vaccines comprising such microorganisms
JPS5645761A (en) Plasma reaction apparatus
MX168908B (en) METHOD FOR REGULATING THE EXPRESSION OF AN EXTERNAL GENE BY CONTROLLING CROP TEMPERATURE AND THE PROCEDURE FOR PRODUCING AN EXTERNAL GENE PRODUCT
GR3027661T3 (en) Treatment of tumors with neurotrophic factors and cell proliferation inhibitors.
Bhattacharya Chromosome damage induced by semicarbazide in spermatocytes of a grasshopper
JPS52114092A (en) Preparation of l-arginine by fermentation process
JPS572684A (en) Preparation of amylase inhibitor ai-b
ES8200886A1 (en) 1-Amino-2-propanol derivatives, process for their preparation and their therapeutic use.