JPH08280282A - Clone seedling culture of eucalyptus globulus - Google Patents

Abstract

PURPOSE: To develop a method for cuttage and improve the efficiency of afforestation in Eucalyptus globulus used as pulp wood. CONSTITUTION: Branches and leaves or regenerated spouts of Eucalyptus globulus grown in the current year are collected to prepare a scion, which is then inserted into a water culture medium and cultured. A scion site of the third to the seventh nodes from the tip of the ear is used as the scion and a water culture medium prepared by mixing a silicate clay in an amount of 50-5 pts.wt. based on water so as to provide >=50wt.% silicon dioxide in the silicate clay is used.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to the cultivation of cloned seedlings by cutting Eucalyptus globulus (E. globulus).
Applied to agriculture and tissue culture.

[0002]

2. Description of the Related Art Eucalyptus is a seed plant that belongs to dicotyledonous plant and angiosperms among angiosperms, and is a member of the family Fusariumaceae (there are about 90 genera including the genus Feijoa of fruit trees). , Eucalyptus plants, and most of them are evergreen broad-leaved trees having a cuticle layer (keratin) on the leaf surface. Of these, eucalyptus globulus (E.glo
is a tree species that is the most widely and afforested in the world, and is also used in Chile and Australia in overseas afforestation projects in Japan's paper industry.

The scientific name is Eucalyptus globulus labile (E
ucalyptus globulus LABIL
L. ). In addition, generally, the genus name is only the initials,
Eucalyptus globulus (E.glob)
Ulus) is often used. Eucalyptus globulus (E. globulus) has been regarded as a different species until then.
i), etc., to make them a subspecies of the species that integrates them, and eucalyptus globulus globulus (E. glob
ulus ssp. globulus).

It is mainly used for pulp, but it is also used as firewood and construction materials. It is said that there are at least 600 tree species in the eucalyptus genus, among which Eucalyptus globulus (E. glob)
ulus) indicates excellent pulp suitability. In other words, volumetric weight, fiber length, which is important in making pulp or paper,
It is very excellent in pulp yield and has been highly evaluated. Although it varies depending on the soil, it has good growth in relatively warm climates.

[0005]

[Problems to be Solved by the Invention]
Globulus (E. globulus) is the most important tree species in terms of pulpwood or forest resources, and is planted in countries around the world. However, since it is difficult to efficiently proliferate by cuttings or tissue culture, it is the current situation that seedlings are grown and afforested. In the case of seedlings, they are genetically non-uniform, so that the growth is uneven and the yield is reduced or the yield is unpredictable. In addition, there is no efficient clonal expansion method even if excellent varieties such as elite trees exist. Very often it is difficult to clone trees,
At present, it is far from practical application even if possible.

[0006] If clonal culture becomes possible, seedlings for planting will be supplied by clonal multiplication of elite trees, it will be possible to increase the amount of growth and to predict the amount of harvest, and it will be possible to carry out planned operations, which will have a great effect. Be expected. Therefore, there is a demand for the rapid establishment of a clonal expansion method for Eucalyptus globulus.

Japanese Unexamined Patent Publication (Kokai) No. 5-236832 discloses that clonal expansion using the shoot primordium method is effective for several species of the genus Eucalyptus. However, this method is a tissue culture method using a rotary incubator, and is an invention that is different from the present invention in terms of method and culture form. In addition, cost performance is also an issue with this method.

With the same intention, Japanese Patent Laid-Open No. 6-1336
In JP 57, it is disclosed that the clonal expansion method of Eucalyptus globulus (E. globulus) using a growing point cut out from an adult tree is effective. This is also a tissue culture, which is completely different from the present invention in the method and culture form.

[0009]

The present inventors have made diligent efforts to solve the above-mentioned problems, and as a result, they have solved the above-mentioned problems by cutting with water as a culture medium, resulting in the following inventions. It was First, eucalyptus globulus (E. glo
of the current year's branches and leaves or regenerated sprouting, and cuttings are made. Then, the cuttings are put into a water medium and cultivated. As the cuttings, the cuttings of the third to seventh sections from the tip of the ears are used, and 50 parts to 5 parts of silicate clay is added to water.
Part, by using a water medium in which 50% by weight or more of silicon dioxide in silicate clay is mixed, it is possible to efficiently obtain seedlings that will survive in the forest in about 4 to 5 months.

That is, the gist of the present invention is to use cuttings from the current year's branches and leaves or regenerated sprouting.
At this time, in particular, the cuttings of the third to seventh sections from the tip of the ear are used, and the silicate clay is 50 to 5 parts to water.
Part, eucalyptus globulus (E. glob) characterized in that seedlings can be obtained efficiently by inserting and culturing in a water culture medium containing 50% by weight or more of silicon dioxide in silicate clay.
ulus) is a method of culturing cloned seedlings by cuttings.

That is, the present invention is a method of cultivating cloned seedlings by vegetative reproduction, and it is possible to supply afforestation seedlings by proliferating excellent varieties by a series of operations for differentiating rhizomes from partial tissues of wood to finish seedlings. As a result, it is possible to increase the initial growth amount and carry out planned operations based on the predictable growth amount.

[0012]

The present invention will be described in more detail. In carrying out the present invention, first, Eucalyptus globulus (E. globul)
Usus) current year's branches and leaves are collected and cut from the bud at the tip into internodes to make ears. The cuttings thus prepared are then inserted into an aqueous medium. As water used for the water culture medium, tap water, ion-exchanged water and distilled water can be used.

As the cuttings, the above-mentioned current-year branches and leaves or regenerated sprouts are used. Then, the cutting parts of the third to seventh sections are used from the tip of the ear cut from them.
Further, an aqueous medium in which 50 to 5 parts by weight of silicate clay and 50% by weight or more of silicon dioxide in silicate clay are used is used. By these, cutting efficiency can be improved.

The cuttings are placed in a well-ventilated place on a light day. Callus is formed from the cut side of the medium 3 to 4 weeks after the start, and rooting is started from the callus after about 3 weeks. Germination starts from the petiole base approximately one month after rooting starts. After that, the new shoots grow and grow to about 20 cm in February, and the seedlings are completed. Seedlings can be produced as many as the number of branches and leaves and regenerated sprouting present in the current year.

[0015] The completed seedlings can be transplanted to the forest land without any acclimation and can be made to live there.

[0016]

EXAMPLES Examples of the present invention will be described in detail below.
The present invention is not limited to these examples.

Example 1 The current year's branches and leaves of Eucalyptus globulus are collected and cut from the tip bud into internodes to prepare cuttings shown in 1 in FIG. The prepared cuttings are inserted into the water medium shown in FIG. 50 in Figure 1
A wide mouth bottle of ml is filled with tap water (shown in 3 in Fig. 1) and a cap of urethane foam (shown in 2 in Fig. 1) is attached to the mouth of the flask so that the cuttings can be made. Use the medium.

The cuttings are placed in a well-ventilated place on a light day. Callus is formed from the cut side of the medium 3 to 4 weeks after the start, and rooting is started from the callus after about 3 weeks. Germination starts from the petiole base approximately one month after rooting starts. After that, the new shoots grow and grow to about 20 cm in February, and the seedlings are completed. In this way, shoots were obtained for each node, and the results are shown in Table 1.

[0019]

[Table 1]

When the completed seedlings were transplanted as they were to the forest without acclimatization, they became viable.

Example 2 Seedlings were prepared in the same manner as in Example 1 except that silicate clay was added to the aqueous medium. The efficiency of seedling formation was examined by varying the number of parts of the added silicate clay to water and the ratio of silicon dioxide in the silicate clay. The results at this time are shown in Table 2. In the table,% is% by weight.

[0022]

[Table 2]

The composition of the silicate clay used in Table 2 is shown in Table 3.

[0024]

[Table 3]

Example 3 Cuttings treated with regenerated sprouts in the same manner as in Example 1 were placed in the same medium as in Example 1, but rooting and germination were also observed, and Eucalyptus globulus (E)・ Globule
It was possible to produce cloned seedlings of (us). The produced seedlings could be transplanted and survived in the forest without acclimation.

Comparative Example 1 Cuttings treated in the same manner as in Example 1 were cut into trees using vermiculite as a medium, but rot progressed from the cut end of the cuttings and rooting was not obtained.

[0027]

EFFECTS OF THE INVENTION According to the present invention, it is possible to efficiently grow cuttings in Eucalyptus globulus, and to supply cloned seedlings of the top trees to the afforestation business. The effect on improving efficiency is enormous.

[Brief description of drawings]

FIG. 1 is a cross-sectional view of an aqueous medium in which cuttings according to Example 1 of the present invention have been performed.

[Explanation of symbols]

1 Cut eucalyptus globulus (E. globul)
2) Urethane foam stopper 3 Tap water 4 Wide mouth bottle

Claims (6)

[Claims] 1. A method for cultivating a cloned seedling of Eucalyptus globulus which grows by cuttings using water as a medium. 2. The clonal seedling culture method according to claim 1, wherein the current year's branches and leaves are used for cuttings. 3. The clone seedling culture method according to claim 1, wherein the regenerated sprouting is used for cuttings. 4. The method for cultivating clone seedlings according to claim 1, 2 or 3, wherein the cuttings of the third to seventh sections from the tip of the ear are used as cuttings. 5. The method for cultivating clone seedlings according to claim 1, 2, 3 or 4, which uses an aqueous medium mixed with silicate clay. 6. The method for cultivating clone seedlings according to claim 5, wherein the silicate clay is contained in an amount of 50 to 5 parts with respect to water and 50% by weight or more of silicon dioxide in the silicate clay is used.