JPH068720Y2 - Bacteria test culture container - Google Patents
Bacteria test culture containerInfo
- Publication number
- JPH068720Y2 JPH068720Y2 JP1984157992U JP15799284U JPH068720Y2 JP H068720 Y2 JPH068720 Y2 JP H068720Y2 JP 1984157992 U JP1984157992 U JP 1984157992U JP 15799284 U JP15799284 U JP 15799284U JP H068720 Y2 JPH068720 Y2 JP H068720Y2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- tank
- bacterial
- container body
- inoculation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【考案の詳細な説明】 〔産業上の利用分野〕 この考案は、臨床検査等において細菌の分離同定及び薬
剤感受性試験等に使用する細菌検査用培養容器に関する
ものである。[Detailed Description of the Invention] [Industrial field of application] The present invention relates to a culture container for bacterial test used for isolation and identification of bacteria and drug susceptibility test in clinical tests and the like.
従来、この種の細菌検査用培養容器には、容器本体の長
手方向の一方側部に複数の菌液接種槽を設け、他方側部
に複数の培養槽を設け、これら菌液接種槽と培養槽とを
適宜間隔を介して連通したものがある。Conventionally, this type of bacterial test culture container is provided with a plurality of bacterial solution inoculation tanks on one side in the longitudinal direction of the container body, and a plurality of culture tanks on the other side, and these bacterial solution inoculation tanks and culture media are provided. There is one that communicates with the tank at an appropriate interval.
しかしながら、前記従来の細菌検査用培養容器は、菌液
接種槽から培養槽への菌液の均等な分液が困難であり、
一定の菌液容量を確保することができなかったり、又菌
液が培養槽から菌液接種槽へ通路を経て逆流するという
問題点を有していた。However, the conventional culture container for bacteria test is difficult to evenly separate the bacterial liquid from the bacterial liquid inoculation tank to the culture tank,
There is a problem in that a certain amount of bacterial liquid cannot be secured, and the bacterial liquid flows backward from the culture tank to the bacterial liquid inoculation tank through the passage.
そこで、この考案は菌液が培養槽から菌液接種槽へ逆流
しない細菌検査用培養容器を提供しようとするものであ
る。Therefore, this invention is intended to provide a culture container for bacterial test in which the bacterial solution does not flow back from the culture tank to the bacterial solution inoculation tank.
この考案は、前記従来の細菌検査用培養容器が有する問
題点を解決するために、容器本体(1)の長手方向の一方
側部に上部が互いに連通した複数の菌液接種槽（４）を
設け、他方側部に各々が独立した複数の培養槽（５）を
設け、これら菌液接種槽（４）と培養槽（５）とを、菌
液接種槽（４）側を緩勾配路（6a）とし培養槽（５）側
を急勾配路（6b）とした起伏通路（６）により連通し、 さらに前記培養槽（５）の上方に当たる位置に小孔（1
1）を設けた被覆フィルム（９）を前記容器本体（１）
の上面に貼着すると共に、 この被覆フィルム（９）に設けた小孔（11）を塞ぐよう
にして被覆フィルム（９）の上面に、測定検出機能を有
する反応材（14）を連設したテープ（12）を貼着したこ
とを特徴とする。In order to solve the problems of the conventional culture container for bacterial test, the present invention provides a plurality of bacterial solution inoculation tanks (4) having upper parts communicating with each other on one side in the longitudinal direction of the container body (1). A plurality of independent culture tanks (5) are provided on the other side, and the bacterial solution inoculation tank (4) and the culture tank (5) are provided on the gentle gradient path ( 6a) and the culturing tank (5) side is communicated by the undulating passage (6) which is a steep slope path (6b), and a small hole (1) is placed at a position above the culturing tank (5).
The covering film (9) provided with 1) is used for the container body (1).
The reaction material (14) having a measurement / detection function is continuously provided on the upper surface of the coating film (9) so as to cover the small holes (11) provided in the coating film (9) while being attached to the upper surface of the coating film (9). It is characterized in that a tape (12) is attached.
前述の手段において、容器本体(1)の長手方向の一方側
部に上部が互いに連通した複数の菌液接種槽（４）を設
けたため、菌液接種槽４から培養槽（５）への菌液の均
等な分液が可能となり、又菌液接種槽（４）と培養槽
（５）とを、菌液接種槽（４）側を緩勾配路（6a）とし
培養槽（５）側を急勾配路（6b）とした起伏通路（６）
により連通したため、菌液が培養槽（５）から菌液接種
槽（６）へ逆流するということがなくなった。In the above-mentioned means, since a plurality of fungal fluid inoculation tanks (4) having upper parts communicating with each other are provided on one side in the longitudinal direction of the container body (1), the fungus from the fungal fluid inoculation tank 4 to the culture tank (5) is The liquid can be evenly separated, and the fungal solution inoculation tank (4) and the culture tank (5) are set to have a gentle gradient path (6a) on the fungal solution inoculation tank (4) side and the culture tank (5) side. Undulating corridor (6) with steep slope (6b)
Since the cells were communicated with each other, the bacterial solution did not flow back from the culture tank (5) to the bacterial solution inoculation tank (6).
また、被覆フィルム（９）に設けた小孔（11）を塞ぐよ
うにして測定検出機能を有する反応材（14）を連設した
テープ（12）を貼着したので、培養終了後に容器本体
（１）を培養槽（５）側に転倒させ、菌液をその反応材
（14）に浸液させることにより、菌の発育をその反応材
（14）で判定することができる。Further, since the tape (12) in which the reaction material (14) having a measuring and detecting function is continuously provided so as to close the small hole (11) provided in the covering film (9), the container body ( By inverting 1) to the culture tank (5) side and immersing the bacterial solution in the reaction material (14), the growth of the bacteria can be determined by the reaction material (14).
以下、この考案の構成を一実施例として示した図面に従
って説明する。The configuration of the present invention will be described below with reference to the drawings shown as an embodiment.
（１）は合成樹脂又はガラス等の材質よりなり好ましく
は透明体とした容器本体であり、その容器本体（１）の
長手方向には等間隔を有して並設された多数の隔壁
（２）により区分された多数の槽（３）が設けられてい
る。前記各々の槽（３）は一方側部を菌液接種槽（４）
とし、他方側部を培養槽（５）としており、断面を略Ｕ
字状とした起伏通路（６）により連通されている。前記
隔壁（２）は、菌液接種槽（４）側を容器本体（１）の
上壁（７）の高さより低く形成し各々の菌液接種槽
（４）をその上部で互いに連通させており、又培養槽
（５）側を容器本体（１）の上壁（７）の高さと略同一
の高さとし各々の独立した培養槽（５）に区分してい
る。さらに、前記起伏通路（６）は、その頂部（８）を
容器本体（１）の上壁（７）の高さより若干低く形成
し、菌液接種槽（４）側を緩勾配路（6a）とし、培養槽
（５）側を急勾配路（6b）としている。尚、図示した培
養槽（５）底部の形状はＵ字状としているが平底状とし
て実施することもできる。(1) is a container body made of a material such as synthetic resin or glass, which is preferably a transparent body, and a large number of partition walls (2) arranged side by side at equal intervals in the longitudinal direction of the container body (1). ), A large number of tanks (3) are provided. Each of the above-mentioned tanks (3) has a bacterial solution inoculation tank (4) on one side.
And the other side is the culture tank (5), and the cross section is approximately U
They are communicated with each other by a undulating passage (6) in the shape of a letter. The partition wall (2) is formed so that the fungus solution inoculation tank (4) side is lower than the height of the upper wall (7) of the container body (1), and the fungus solution inoculation tanks (4) are communicated with each other at the upper part thereof. The height of the culture tank (5) is approximately the same as the height of the upper wall (7) of the container body (1), and the culture tank (5) is divided into independent culture tanks (5). Further, the undulation passageway (6) has its top portion (8) formed slightly lower than the height of the upper wall (7) of the container body (1), and the bacterial inoculation tank (4) side is provided with a gentle slope passage (6a). The steep slope path (6b) is on the side of the culture tank (5). The shape of the bottom of the culture tank (5) shown in the figure is U-shaped, but it may be flat-bottomed.
（９）は柔軟性を有する合成樹脂等の透明な材質よりな
る被覆フィルムであり、この被覆フィルム（９）の周囲
又は前記容器本体（１）上面の周囲に粘着槽（10）を設
け、被覆フィルム（９）を容器本体（１）上面に貼着し
ている。さらに、この被覆フィルム（９）には、前記各
々の培養槽（５）の上方に当る位置に小孔（11）を設け
ている。尚、被覆フィルム（９）は一部分を剥ぎ取る構
造としても実施することができる。(9) is a covering film made of a transparent material such as a flexible synthetic resin, and an adhesive tank (10) is provided around the covering film (9) or around the upper surface of the container body (1) to cover it. The film (9) is attached to the upper surface of the container body (1). Further, the coating film (9) is provided with a small hole (11) at a position above the culture tanks (5). The covering film (9) can also be implemented as a structure in which a part is peeled off.
（12）は柔軟性を有する合成樹脂等の透明な材質よりな
るテープであり、このテープ（12）の周囲又は前記被覆
フィルム（９）上面の周囲に粘着層（13）を設け、被覆
フィルム（９）に設けた小孔（11）を塞ぐようにして被
覆フィルム（９）の上面にテープ（12）を貼着してい
る。又、前記テープ（12）には発色試薬等の多種類の測
定検出機能を有するディスク等の反応材（14）を着脱自
在に連設して、前記被覆フィルム（９）の小孔（11）上
にこの反応材（14）を配置させることもできる。(12) is a tape made of a transparent material such as a flexible synthetic resin. An adhesive layer (13) is provided around the tape (12) or around the upper surface of the covering film (9) to form a covering film ( A tape (12) is attached to the upper surface of the covering film (9) so as to close the small hole (11) provided in 9). Further, a reaction material (14) such as a disc having various kinds of measurement and detection functions such as a coloring reagent is removably connected to the tape (12), and the small hole (11) of the covering film (9) is attached. The reaction material (14) can be placed on the top.
上記構成としたこの実施例の細菌検査用培養容器は、あ
らかじめ各々の培養槽（５）の菌鑑別用の培地、基質等
を乾燥あるいは生の状態で配置しておき、容器本体
（１）に貼着した被覆フィルム（９）の一部を剥がして
菌液接種槽（４）に一定量の菌液を分注し、容器本体
（１）を左右に揺動させて菌液を均等にならし、容器本
体（１）の菌液接種槽（４）側を持ち上げて、菌液を各
々の培養槽（５）に流入させて接種したのち、常法に従
い培養することにより、細菌の同定検査に応用すること
ができる。又、各々の培養槽（５）に薬剤感受性試験用
培地を各種濃度調整し、菌液接種槽（４）には一定量の
菌液を分注し、前記と同様の操作をすることにより、細
菌の薬剤感受性試験ならびに薬剤の最小発育阻止濃度測
定試験に応用することができる。尚、前記菌液接種槽
（４）側の緩勾配路（6a）には親水性界面活性剤を塗布
あるいはコロナ放電処理を施こし、培養槽（５）側の急
勾配路（6b）には疎水性界面滑性剤を塗布することによ
り、菌液の菌液接種槽（４）での分液精度の向上ならび
に菌液の培養槽（５）での保持能力を確実にすると共
に、培養槽（５）から菌液接種槽（４）への菌液の逆流
を完全に阻止することができる。In the culture container for bacterial test of this example having the above-mentioned configuration, a culture medium for identifying bacteria in each culture tank (5), a substrate and the like are arranged in a dried or raw state in advance, and the container body (1) is placed in the container body (1). A part of the applied covering film (9) is peeled off, a certain amount of the bacterial solution is dispensed into the bacterial solution inoculation tank (4), and the container body (1) is swung left and right to evenly distribute the bacterial solution. Then, the bacterial solution inoculation tank (4) side of the container body (1) is lifted, the bacterial solution is allowed to flow into each of the culture tanks (5) for inoculation, and then cultured according to a conventional method to identify bacteria. Can be applied to. Further, by adjusting various concentrations of the medium for drug susceptibility test in each culture tank (5), dispensing a fixed amount of the bacterial solution in the bacterial solution inoculation tank (4), and performing the same operation as described above, It can be applied to a drug sensitivity test of bacteria and a minimum inhibitory concentration measurement test of a drug. The gentle gradient path (6a) on the bacterial inoculation tank (4) side was coated with a hydrophilic surfactant or subjected to corona discharge treatment, and the steep gradient path (6b) on the culture tank (5) side was applied. By applying a hydrophobic interfacial lubricant, it is possible to improve the accuracy of liquid separation of the bacterial liquid in the bacterial liquid inoculation tank (4) and to ensure the retention capacity of the bacterial liquid in the culture tank (5), as well as the culture tank. It is possible to completely prevent the backflow of the bacterial solution from (5) to the bacterial solution inoculation tank (4).
さらに、この実施例の培養容器は、被覆フィルム（９）
に貼着したテープ（12）を剥がして、被覆フィルム
（９）に設けた小孔（11）より培養槽（５）にバイオセ
ンサーを挿入することにより、酸素等の比活性を測定す
ることが可能である。又テープ（12）に発色試薬等を含
ませた反応材（14）を連設し、前記被覆フィルム（９）
に設けた小孔（11）上にこのディスク（14）を配置させ
てある場合には、培養終了後に容器本体（１）を培養槽
（５）側に転倒させ、菌液をその反応材（14）に浸液さ
せることにより、菌の発育をその反応材（14）で判定す
ることができる。尚、この場合培養終了後に菌液と反応
材（14）が接触することになるので、ある種の発色試薬
により発育の阻害を受ける性質を有する細菌の薬剤感受
性測定も難無く行うことができる。さらに、容器本体
（１）を透明体とした場合には、菌液の比濁を測光光度
計、比色計によって光学的に測定することにより、菌の
発育を検査することができる。Furthermore, the culture vessel of this example has a coated film (9).
It is possible to measure the specific activity of oxygen and the like by peeling off the tape (12) attached to the membrane and inserting the biosensor into the culture tank (5) through the small hole (11) provided in the covering film (9). It is possible. Further, the tape (12) is provided with a reaction material (14) containing a coloring reagent or the like, and the coating film (9)
When the disc (14) is placed on the small hole (11) provided in the container, the container body (1) is turned over to the culture tank (5) side after completion of the culture, and the bacterial solution is mixed with the reaction material ( By soaking it in 14), it is possible to determine the growth of the bacterium with the reaction material (14). In this case, since the bacterial solution and the reaction material (14) come into contact with each other after the completion of the culture, the drug sensitivity of bacteria having the property of inhibiting the growth by a certain coloring reagent can be easily measured. Further, when the container body (1) is made transparent, the growth of bacteria can be inspected by optically measuring the turbidity of the bacterial solution with a photometric photometer and a colorimeter.
また、従来の細菌検査用培養容器は、多種類の測定検出
機能を有しておらず、多くの検査項目を扱う場合には非
常に不便であったが、この実施例の細菌検査用培養容器
は、培養槽（５）の上方に当る位置に小孔（11）を設け
た被覆フィルム（９）を容器本体（１）の上面に貼着す
ると共に、被覆フィルム（９）の上面に多種類の測定検
出機能を有する反応材（14）を連設したテープ（12）を
貼着したため、多くの検査項目を扱う場合にも非常に便
利であるという利点がある。In addition, the conventional culture container for bacteria test does not have many kinds of measurement and detection functions, which is very inconvenient when handling many test items, but the culture container for bacteria test of this example Adheres a covering film (9) having a small hole (11) at a position above the culture tank (5) to the upper surface of the container body (1), and at the same time, has various kinds on the upper surface of the covering film (9). Since the tape (12) in which the reaction material (14) having the measurement and detection function is connected is attached, it is very convenient even when handling many inspection items.
尚、さらにこの実施例の細菌検査用培養容器は、容器本
体（１）の上面に被覆フィルム（９）を貼着しているの
で、培地の乾燥を防ぎ、抗酸菌など比較的長期の培養に
適するほか、被覆フィルム（９）を一部開放することに
より、ＣＯ２培養装置あるいは嫌気培養装置等での培養
が可能である等の利点がある。In addition, in the culture container for bacteria test of this Example, since the coating film (9) is adhered to the upper surface of the container body (1), the culture medium is prevented from being dried and the acid-fast bacterium and the like are cultured for a relatively long time. In addition, there is an advantage that the coating film (9) can be partially opened to allow culturing in a CO 2 culturing device or an anaerobic culturing device.
以上の述べた如く、この考案の細菌検査用培養容器は、
菌液接種槽（４）から培養槽（５）への菌液の均等な分
液が可能となり、又菌液が培養槽（５）から菌液接種槽
（４）へ逆流するということがなくなるという優れた効
果を有する。As described above, the culture container for bacterial test of the present invention is
Even distribution of the bacterial liquid from the bacterial liquid inoculation tank (4) to the culture liquid tank (5) is possible, and the bacterial liquid liquid does not flow back from the culture liquid tank (5) to the bacterial liquid liquid inoculation tank (4). It has an excellent effect.
また、被覆フィルム（９）に設けた小孔（11）を塞ぐよ
うにして測定検出機能を有する反応材（14）を連設した
テープ（12）を貼着したので、培養終了後に容器本体
（１）を培養槽（５）側に転倒させ、菌液をその反応材
（14）に浸液させることにより、菌の発育をその反応材
（14）で判定することができる。この場合、培養終了後
に菌液と反応材（14）とが接触することになるので、あ
る種の反応材（14）により発育の阻害を受ける性質を有
する細菌の薬剤感受性測定を難無く行うことができると
いう効果を有する。Further, since the tape (12) in which the reaction material (14) having a measuring and detecting function is continuously provided so as to close the small hole (11) provided in the covering film (9), the container body ( By inverting 1) to the culture tank (5) side and immersing the bacterial solution in the reaction material (14), the growth of the bacteria can be determined by the reaction material (14). In this case, since the bacterial solution and the reaction material (14) come into contact with each other after the culture is completed, it is possible to easily carry out the drug sensitivity measurement of the bacteria having the property that the growth is inhibited by the certain reaction material (14). It has the effect of being able to.
図面はこの考案に係る細菌検査用培養容器の一実施例を
示すもので、第１図は被覆フィルムとテープの一部を剥
がした状態を示すその全体斜視図、第２図はその部分拡
大平面図、第３図は第２図中のＡ−Ａ線による断面図、
第４図は第２図中のＢ−Ｂ線による断面図、第５図は第
２図中のＣ−Ｃ線による断面図である。 （１）……容器本体、（４）……菌液接種槽、（５）…
…培養槽、（６）……起伏通路、（6a）……緩勾配路、
（6b）……急勾配路、（９）……被覆フィルム、（11）
……小孔、（12）……テープ、（14）……反応材FIG. 1 shows an embodiment of a culture container for bacteria test according to the present invention. FIG. 1 is an overall perspective view showing a state where a covering film and a tape are partly removed, and FIG. 2 is a partially enlarged plan view thereof. Fig. 3 is a sectional view taken along the line AA in Fig. 2,
4 is a sectional view taken along the line BB in FIG. 2, and FIG. 5 is a sectional view taken along the line CC in FIG. (1) …… Container body, (4) …… Bacterial fluid inoculation tank, (5)…
… Cultivation tank, (6) …… Relief passage, (6a) …… Slope road,
(6b) ... steep slope, (9) ... coating film, (11)
…… Small hole, (12) …… tape, (14) …… reactive material
───────────────────────────────────────────────────── フロントページの続き (72)考案者 菅原 和行 長崎県長崎市若草町15―10 市場ビル204 号 (56)参考文献 実開 昭57−149800（ＪＰ，Ｕ) ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kazuyuki Sugawara No.204 Market Building, 15-10 Wakakusacho, Nagasaki City, Nagasaki Prefecture, No.204 (56) Bibliographic Reference Sho 57-149800 (JP, U)
Claims (1)
部が互いに連通した複数の菌液接種槽（４）を設け、他
方側部に各々が独立した複数の培養槽（５）を設け、こ
れら菌液接種槽（４）と培養槽（５）とを、菌液接種槽
（４）側を緩勾配路（6a）とし培養槽（５）側を急勾配
路（6b）とした起伏通路（６）により連通し、 さらに前記培養槽（５）の上方に当たる位置に小孔（1
1）を設けた被覆フィルム（９）を前記容器本体（１）
の上面に貼着すると共に、 この被覆フィルム（９）に設けた小孔（11）を塞ぐよう
にして被覆フィルム（９）の上面に、測定検出機能を有
する反応材（14）を連設したテープ（12）を貼着したこ
とを特徴とする細菌検査用培養容器。1. A container body (1) is provided with a plurality of fungal inoculation tanks (4) having upper portions communicating with each other on one side in the longitudinal direction, and a plurality of independent culture tanks (5) on the other side. The bacterial solution inoculation tank (4) and the culture tank (5) are provided with a gentle gradient path (6a) on the bacterial solution inoculation tank (4) side and a steep gradient path (6b) on the culture tank (5) side. Through the undulating passages (6), and a small hole (1) at a position above the culture tank (5).
The covering film (9) provided with 1) is used for the container body (1).
The reaction material (14) having a measurement / detection function is continuously provided on the upper surface of the coating film (9) so as to cover the small holes (11) provided in the coating film (9) while being attached to the upper surface of the coating film (9). A culture container for testing bacteria, characterized by having a tape (12) attached.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1984157992U JPH068720Y2 (en) | 1984-10-18 | 1984-10-18 | Bacteria test culture container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1984157992U JPH068720Y2 (en) | 1984-10-18 | 1984-10-18 | Bacteria test culture container |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6172200U JPS6172200U (en) | 1986-05-16 |
| JPH068720Y2 true JPH068720Y2 (en) | 1994-03-09 |
Family
ID=30715978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1984157992U Expired - Lifetime JPH068720Y2 (en) | 1984-10-18 | 1984-10-18 | Bacteria test culture container |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH068720Y2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5244630A (en) * | 1988-04-22 | 1993-09-14 | Abbott Laboratories | Device for performing solid-phase diagnostic assay |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6016320Y2 (en) * | 1981-03-18 | 1985-05-21 | 栄研化学株式会社 | Bacteria identification tray |
-
1984
- 1984-10-18 JP JP1984157992U patent/JPH068720Y2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6172200U (en) | 1986-05-16 |
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