JPH06323971A - Stirring device for mixed liquid and sample introducing device - Google Patents

Abstract

PURPOSE:To stir the liquid contained in a container effectively by imparting rotation to the container, which is held with a holding part comprising several rubber rollers. CONSTITUTION:A holding part for holding a mixing container has the constitution, wherein a rubber roller 16 is attached to a supporting body 17, one of rubber rollers 28 is attached to the supporting body 17 and one is attached to a supporting body 27. When a stepping motor 22 is rotated, rotation is imparted to a teeth part 33 through a gear 32. The supporting body 17 is moved along a groove 34 to a position, where the holding part comprising the rubber roller 16 and the rubber rollers 28 holds the mixing container. When a motor 21 is rotated, the rubber roller 16 is rotated through a pulley 31, a belt 20 and a pulley 18. When the rubber roller 16 is rotated, the mixing container, which is held with the holding part comprising the rubber roller 16 and the rubber rollers 28, is rotated, and the sample contained in the mixing container is mixed and stirred.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mixed liquid stirring device and a sample introduction device using the same, in which the liquid to be mixed contained in the container can be stirred by rotating the container itself. The present invention relates to an apparatus and a sample introduction apparatus using the apparatus.

[0002]

2. Description of the Related Art A conventional stirring device is as follows.

The needle is moved to below the liquid level in the container containing the liquid to be mixed, and after sucking the liquid in the container, the needle is moved to above the liquid level and the liquid sucked into the needle is discharged. The liquid is stirred by repeating the above operation a plurality of times.

After sucking air into the suction / exhaust nozzle connected to the metering pump, a proper amount of reagent and sample is sucked, the tip of the suction / discharge nozzle is inserted up to near the bottom surface of the processing container, and the sample / reagent and air inside the suction / discharge nozzle are sucked. By ejecting and, the sample and the reagent are agitated by the air that is ejected when the tip of the nozzle is positioned in the solution of the ejected sample and the reagent.

After picking up the container containing the liquids to be mixed with the hand, the hand is placed in the vertical plane 1
Reciprocating rotation is repeated within an angle of 20 degrees. With the reciprocating rotation of the hand, the container is repeatedly inverted, and the liquid contained in the container is agitated.

The stirring rod is inserted into the container containing the liquids to be mixed, and the stirring rod is rotated by the motor to stir the liquid contained in the container.

[0007]

In the above conventional methods, and, the stirring efficiency is poor, and it is necessary to increase the number of repetitions of suction and discharge in order to perform sufficient mixing and stirring. Requires a mechanism of a hand for holding a liquid, a mechanism for holding the container with the hand, and a mechanism for lifting the hand holding the container, which complicates the device mechanism. The method of (1) requires the opening of the upper part of the container in order to insert the stirring rod into the container containing the liquid, and cannot be used for a sealed container. Further, it is necessary to wash the stirring rod after stirring the liquid.

An object of the present invention is to provide a mixed liquid stirring device and a sample which can effectively stir the liquid contained in the container regardless of the hermeticity of the container by a mechanism simpler than the device of the container overturning mechanism. To provide an introducing device.

[0009]

In order to achieve the above-mentioned object, according to the present invention, a grip portion composed of three rubber rollers for sandwiching a container containing a liquid to be stirred, and a container containing the liquid in the grip portion. In order to transmit the rotation of the motor to the slide mechanism that moves to the position, and the rotation of the motor to one of the three rubber rollers of the grip, a belt is provided between the motor and the one rubber roller. . A spring is provided between two rubber rollers other than one rubber roller provided with a belt in the three rubber rollers of the grip portion, and the container containing the liquid has three springs in the grip portion due to the force of the spring. There is a pinch in between.

[0010]

According to the above construction, when the container containing the liquid to be stirred is sandwiched between the grips composed of the three rubber rollers and then the motor is rotated, the rotation of the motor causes the rotation of the gripper to reach 3 It is transmitted to one of the three rubber rollers, and further, the rotation is transmitted to the container sandwiched by the grip portion composed of three rubber rollers, and the container rotates. As the container rotates, the liquid contained in the container is agitated.

[0011]

Embodiments of the present invention will be described below with reference to the drawings.

In FIG. 1, a rack 26 is provided with a sample container 8 containing a liquid sample 24, a reagent container 9 containing a liquid reagent 25, and a mixing container 10. The freely moving needle 11 is connected to a syringe 14 via a three-way switching valve 13. 3-way switching valve 1
A cleaning liquid 15 is connected to one side of 3 and overflows in the cleaning port 7 to fill the inner diameter of the needle 11 and clean the inner and outer walls. Further, the sampling flow path of the injection valve 4 is cleaned via the injection port 6. The mobile phase 1 sent by the pump 2 is connected to the column 3 through the sample loop 5 of the injection valve 4. A rotation mechanism unit A is installed to rotate the mixing container 10. FIG. 2 shows a detailed view of the rotation mechanism section A. The gripping portion for sandwiching the mixing container 10 is configured such that the rubber roller 16 is attached to the support body 17, one of the rubber rollers 28 is attached to the support body 17, and one is attached to the support body 27. The support body 27 is attached to the support body 17 at a rotation shaft 37, and is pulled by the support body 17 by a spring 29 attached to the rotation shaft 41. The support 17 is slidably attached to the groove 34 of the block 23. A stepping motor 22 and a rotation motor 21 are attached to the block 23. A gear 32 is attached to the stepping motor 22 and meshes with a toothed portion 33 of the support 17. When the stepping motor 22 is rotated, the rotation is transmitted to the tooth-like portion 33 of the support 17 via the gear 32, and the support 17 can be slid toward the mixing container 10 or in the opposite direction. The position of the support 17 is determined by controlling the number of steps of the stepping motor 22. A pulley 31 is attached to the rotation motor 21. A pulley 19 is provided between the pulley 31 and the pulley 18.
The belt 20 is attached via. Pulley 19
The belt 20 is attached to the belt 20 while being pulled by the spring 30 between the belt 20 and the spring fixing point 42.
Prevents 0 cutting and relaxation. When the rotation motor 21 is rotated, the rotation is transmitted to the rubber roller 16 via the pulley 31, the belt 20 and the pulley 18. The rotation mechanism section A is shown in FIG.
The slide mechanism of is shown. The slide projection 43 of the rotation mechanism A is fitted in the slide groove 35. The rotation mechanism portion A is attached to the toothed belt 36. The toothed belt 36 is wound around a gear 42. Motor 3
A gear 38 is attached to the gear 9, and the gear 38 meshes with the toothed belt 36. When the stepping motor 39 is rotated, the toothed belt 36 moves via the gear 38, and the rotation mechanism portion attached to the toothed belt 36 moves along the slide groove 35. The position of the rotation mechanism section A is determined by controlling the number of steps of the stepping motor 39.

In the above state, after moving the needle 11 to the position of the sample container 8, the needle 11 is lowered to below the liquid surface of the sample container 8 and the liquid sample 24 is sucked by a set amount. After moving the needle 11 up to the position of the mixing container 10, the needle 11 is lowered inside the mixing container 10, and the liquid sample 24 is discharged in a set amount. After raising the needle 11 and moving it to the washing port 7, the syringe 14 is operated to feed the washing liquid 15 to wash the inner and outer walls of the needle 11. After moving the needle 11 to the position of the reagent container 9, the needle 11 is lowered to below the liquid surface of the sample container 9, and the liquid sample 25
Aspirate a set amount. Raise needle 11 to raise mixing container 1
After moving to the position of 0, it is lowered into the inside of the mixing container 10 and the liquid sample 25 is discharged in a set amount. After raising the needle 11 and moving it to the washing port 7, the syringe 14 is operated to feed the washing liquid 15 to wash the inner and outer walls of the needle 11. When the stepping motor 39 is rotated, the gear 38
The toothed belt 36 moves via the, and the rotation mechanism portion A attached to the toothed belt 36 moves to a position parallel to the mixing container 10 along the slide groove 35. When the stepping motor 22 is rotated, the rotation is transmitted to the tooth-shaped portion 33 via the gear 32, and the support 17 is placed in the groove 34 until the gripping portion composed of the rubber roller 16 and the rubber roller 28 sandwiches the mixing container 10. Move along. When the motor 21 is rotated, the pulley 31 and the belt 2
0, the rubber roller 16 rotates via the pulley 18. With the rotation of the rubber roller 16, the rubber roller 16
Further, the mixing container 10 sandwiched between the gripping portions composed of the rubber roller 28 is rotated, and the sample contained in the mixing container 10 is mixed and stirred. If the rotation in the opposite direction is repeated instead of the rotation in only one direction, the stirring efficiency further increases. After sufficiently mixing and stirring the sample, the rotation of the motor 21 is stopped. Rotate the stepping motor 22 to rotate the gear 3
The rotation is transmitted to the tooth-shaped portion 33 via 2 to move the support 17 to the original position. Mixing needle 10 with needle 11
After moving to the position of, the sample is sucked down by a set amount by descending below the liquid surface of the mixing container 10. The needle 11 is moved to the injection port 6 and the injection valve 4 is switched to the sample injection state, and then the syringe 14 is operated to inject the sample into the sample loop 5 at a set amount. When the injection valve 4 is switched from the pump 2 to the flow path connecting to the column 3 via the sample loop 5, the sample in the sample loop is introduced into the mobile phase liquid transfer path. The needle 11 is moved to the washing port 7, the syringe 14 is operated, and the washing liquid 15 is sent to wash the inner and outer walls of the needle 11. The needle 11 is moved to the injection port 6, and the sample injection line is washed. FIG. 4 shows a measurement flow of the orthophthalaldehyde-modified amino acid analysis. FIG. 5 shows the results of five consecutive measurements of the orthophthalaldehyde-modified amino acid analysis according to the measurement flow of FIG. 4 according to this example. FIG. 6 shows the results of five consecutive measurements of the orthophthalaldehyde-modified amino acid analysis according to the measurement flow of FIG. 4 by agitation using a needle and stirring using discharge. The analysis results of this example give good reproducibility.

According to this embodiment, the sample and the reagent can be effectively stirred, and the sample sufficiently mixed and stirred can be introduced into the analyzer, so that the reproducibility of the analysis result is improved.

[0015]

According to the present invention, the container containing the liquid to be mixed is sandwiched by the gripping portion composed of the rubber roller, and the container is rotated in accordance with the rotation of the rubber roller to be contained in the container. The resulting liquid can be agitated and mixed. In the sample introduction device, a sample that has been sufficiently mixed with stirring can be introduced into the analyzer, and measurement results with good reproducibility can be obtained.

[Brief description of drawings]

FIG. 1 is a configuration diagram of a sample introduction device.

FIG. 2 is a detailed view of a rotation mechanism section A.

FIG. 3 is a view showing a slide mechanism of a rotation mechanism section A.

FIG. 4 is a measurement flow chart of an orthophthalaldehyde-modified amino acid analysis.

FIG. 5 is a diagram showing a result of a round measurement of an amino acid modified with orthophthalaldehyde according to the present example.

FIG. 6 is a diagram showing the measurement results of orthophthalaldehyde-modified amino acid analysis by stirring using suction and discharge with a needle.

[Explanation of Codes] 1 ... Eluent, 2 ... Pump, 3 ... Column, 4 ... Injection valve, 5 ... Sample loop, 6 ... Injection port, 7 ... Wash port, 8 ... Sample container, 9 ... Reagent container, 10 ... Mixing container,
11 ... Needle, 12 ... Connection tube, 13 ... 3-way switching valve, 14 ... Syringe, 15 ... Wash solution, 16, 2
8 ... Rubber roller, 17, 27 ... Support, 18, 19,
31 ... Pulley, 20 ... Belt, 21 ... Motor, 22, 3
9 ... Stepping motor, 23 ... Block, 24 ... Sample, 25 ... Reagent, 26 ... Rack, 29, 30 ... Spring, 3
2, 38, 42 ... Gears, 33 ... Wavy portions, 34, 35,
40 ... Grooves, 36 ... Toothed belts, 37, 41 ... Rotating shafts, 43 ... Sliding protrusions.

Claims (2)

[Claims] 1. A rotating mechanism for a mixed liquid, comprising: a container containing liquids to be mixed; a gripping part comprising a rubber roller for sandwiching and fixing the container; and a rotating mechanism for rotating the rubber roller. With the rotation of the rubber roller of the grip portion, the container sandwiched by the grip portion is rotated, and the liquids to be mixed contained in the container are mixed and stirred. Mixing device stirring device. 2. A mechanism of the mixed liquid stirring device according to claim 1,
A sample container containing a liquid sample, a reagent container containing a liquid reagent, a mixing container that receives the liquid sample and the liquid reagent, and a needle for dispensing the liquid sample and the liquid reagent into the mixing container. In the sample introduction device provided with an introduction unit for introducing the mixed sample in the mixing container into the analyzer flow path, after the liquid sample and the liquid reagent are dispensed into the mixing container by the needle. A sample introduction device, characterized in that the liquid container and the liquid reagent contained in the mixing container are stirred and mixed by rotating the mixing container by the mechanism of claim 1.