JPH0582299U - Culture instrument - Google Patents

Abstract

(57) [Summary] [Structure] The container 2 is composed of a main body provided with a culture part 4 having a mortar-shaped recess 3 and a lid 1, and the top of the culture part is a peripheral wall 5 of the container. It is lower than the top, and the bottom of the recess is at the same position as or higher than the bottom of the container and has a flat or shallow spherical shape, and the sloping inner wall of the recess is straight and the bottom of the container is For 30 ~
It makes an angle of 60 degrees. [Effect] Paraffin oil is not required to suppress the evaporation of the culture solution when culturing animal eggs, there is no change in medium components due to the elution of paraffin, and pipette operation is easy. You can reach everyone.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention mainly relates to a culture instrument used for culturing animal eggs, fertilized eggs, and egg operations.

[0002]

[Prior Art]

 Generally, for culturing animal eggs, microdroplets of the culture solution are made on the bottom of a small petri dish, and the microdroplets are covered with paraffin oil to suppress evaporation of these microdroplets, and the eggs are cultured in the microdroplets. , A microdrop culture method that uses an egg is used. This method requires a large amount of paraffin oil for the actual amount of the culture solution, and since the microdroplets of the culture solution are easily broken, it requires careful handling, and the paraffin oil should not be used in paraffin oil. In order to prevent changes in the medium components due to the dissolution of the culture medium components, it is necessary to prepare the paraffin oil to be used by stirring it with the culture medium and saturating it.

In addition, when transferring eggs or a culture solution, a Pasteur pipette or the like is used under a stereoscopic microscope, but when microdroplets are located at the corners of the bottom and side walls of the Petri dish. Had a problem in that the stereomicroscope interfered and the pipette could not be stood up, so the tip of the pipette could not reach the microdroplets, and the operation could not be performed.

[0004]

[Problems to be solved by the device]

 The present invention was devised in view of such drawbacks in the microdrop method using a Petri dish, and minimizes the amount of paraffin for suppressing the evaporation of the culture solution, which may cause the culture solution to collapse. It is an object of the present invention to provide a culture instrument that does not need to be handled, is easy to handle, and is easy to pipette when operating eggs.

[0005]

Means for Solving the Problems That is, the present invention is composed of a main body portion provided with a culture portion having a mortar-shaped recess in a substantially central portion of a petri dish-shaped container, and a lid covering the main body portion. , The top of the culture part is lower than the top of the peripheral wall of the container, and the bottom of the mortar-shaped recess is at the same position as or higher than the bottom of the container and has a flat or shallow spherical shape. The culturing device is characterized in that the mortar-shaped recess has a side cross-sectional shape in which the slanted inner wall is linear and the angle with the bottom of the container is in the range of 30 to 60 degrees.

Hereinafter, the present invention will be described in detail with reference to the drawings. FIG. 1 is a perspective view showing an example of a culture instrument of the present invention. (A) is a lid and (b) is a main body having a culture part. FIG. 2 is a side sectional view showing an embodiment of the structure of the culture instrument body.

As shown in FIG. 1 (b), the culture instrument main body of the present invention has a culturing part (3) having a mortar-shaped recess (3) substantially at the center of a petri dish-shaped container (2). 4) is provided, by contrast, the lid (1) shown in FIG. 1 (a) loosely covers the entire container (2) of the main body. The top of the culture section (4) is lower than the top of the peripheral wall (5) of the vessel (2), and the bottom of the recess (3) is at the same level as or higher than the bottom of the vessel (2). It has a flat or shallow spherical shape.

The slanted inner wall of the mortar-shaped recess (3) has a linear cross section, and the angle formed with respect to the bottom surface of the container (2) is preferably in the range of 30 to 60 degrees. If this angle is larger than 60 degrees, the stereoscopic microscope interferes with the pipette operation under the stereoscopic microscope and the tip of the pipette cannot reach the corner of the bottom of the recess (3). On the other hand, when the angle is less than 30 degrees, the recess of the recess (3) becomes shallow and the volume becomes small, so that a sufficient amount of the culture solution cannot be stored.

The lid (1), the container (2), and the culture section (4) are all plastic molded products, and their materials are transparent enough to be confirmed by a microscope. There is no particular limitation as long as it has requirements such as damage resistance, good water resistance, and easy molding. Examples of such plastics include polystyrene, TPX resin, polycarbonate, and acrylic resin.

In addition, in the method of providing the culture section (4) in the central portion of the container (2), the container (2) and the culture section (4) are separately prepared, and as shown in FIG. It may be attached by a method such as an adhesive or ultrasonic welding, or may be integrally formed from the beginning into the shape shown in FIG. 2 (b). The container and the culture section are generally circular in shape, but the shape is not necessarily limited to circular.

Next, a method of using the culture instrument of the present invention will be described. First, the outside of the culture section (4) in the container (2) is filled with water. At this time, the temperature of the water is preferably 37 ° C. in order to keep the culture solution filled in the culture section (4) warm. After that, fill the recess (3) with an appropriate amount of culture solution, place it on a heating plate set at 37 ° C, transfer the collected eggs into the culture solution, and wash the eggs by pipetting etc. Operate. When culturing eggs, cover them with the lid (1) and place them in an incubator.

By filling the outside of the culture part (4) of the container (2) with water, an appropriate amount of moisture is replenished to the culture liquid in the recess (3), so that the evaporation of the culture liquid can be suppressed, and It is not necessary to cover the culture medium with paraffin oil. From this point of view, when the ratio of the area of the culture section (4) to the inner area of the container (2) (the inner area of the top of the culture section) is examined, the ratio of the culture section area is not necessarily strict, It is preferably about 20 to 45%, and more preferably about 30%. If this ratio is made small, it is preferable to suppress the evaporation of the culture solution, but if it is 20% or less, the space efficiency becomes poor, and if it exceeds 45%, the effect of suppressing the evaporation of the culture solution becomes insufficient.

[0013]

[Effect of the device]

 By using the culture instrument according to the present invention, it is not necessary to cover the culture solution with paraffin oil in order to suppress the evaporation of the culture solution in the culture of animal eggs, which may cause the culture solution to collapse in the paraffin oil. Since it is not necessary, it is easy to handle, and it is also easy to operate with a pipette such as washing eggs, and the tip of the pipette can reach every corner of the culture part, a culture instrument used for culturing animal eggs It is suitable as

[Brief description of drawings]

FIG. 1 is a perspective view of a culture instrument according to an embodiment of the present invention, in which (a) shows a lid and (b) shows a container body provided with a culture section.

FIG. 2 is a side sectional view showing an embodiment of the structure of the culture instrument body.

[Explanation of symbols]

 1 Lid 2 Container 3 Recess 4 Culture Section 5 Peripheral Wall

Claims (1)

[Scope of utility model registration request] 1. A body part having a culturing part having a mortar-shaped recess in a substantially central part of a petri dish-shaped container,
It comprises a lid for covering the main body, the top of the culture section is lower than the top of the peripheral wall of the container, and the bottom of the mortar-shaped recess is the same as or higher than the bottom of the container. And a flat or shallow spherical shape, and the side cross-sectional shape of the mortar-shaped recess has a slanted inner wall that is linear, and the angle with the bottom of the container is in the range of 30 to 60 degrees. Equipment.