JP5537352B2 - Hair growth inhibitor - Google Patents

Description

  The present invention relates to a hair growth inhibitor.

  Biologically, hair or body hair protects important organs such as the head, chest, and limbs. However, in recent years, there is an increasing tendency that hair on limbs and the like is preferably not in terms of aesthetic appearance.

  Examples of the method for removing body hair include a mechanical removal method using a shaver, a hair removal device, and the like, and a removal method by chemical action using a hair removal agent or a hair removal agent. However, these hair removal methods may be accompanied by physical or chemical irritation to the skin and are still insufficient in terms of hair suppression action, so that the hair removal treatment is performed again after a certain period of time. There must be. Reduction of body hair removal processing is desired.

Beetle ( Cynanchum atratum Bge.) Is a perennial plant belonging to the genus Camellia, and its root is used in traditional Chinese medicines such as Hakuba-to. Beech roses are known to have medicinal effects such as clear fever, antipyretic, diuretic action and the like, and Cynanchol having a cardiac glycoside action is known as its medicinal ingredient.

  Patent Document 1 discloses a herbal extract for skin growth containing a herbal extract containing white rose and an active ingredient thereof, a herbal extract having an angiogenic activity and an active ingredient thereof. Yes. Patent Document 2 describes the use of a composition containing an extract of a plant belonging to the genus Calyceae for cosmetic treatment of human skin including inhibition, suppression or delay of hair growth. However, it was not clear what the effect of beech roses alone on hair growth.

JP 2003-221313 A Special Table 2007-517824

  The present invention relates to a hair growth inhibitor containing beech grass or an extract thereof as an active ingredient.

  As a result of searching for a substance that suppresses hair growth, the present inventors have found that beech grass or its extract has an effect of suppressing hair growth, and have completed the present invention.

That is, the present invention provides the following.
1) A hair growth inhibitor containing beech grass or an extract thereof as an active ingredient.
2) The hair growth inhibitor according to (1), wherein the extract is a water extract or an aqueous ethanol extract.

  According to the present invention, a plant-derived hair growth inhibitor that is safe and effective for a living body can be provided.

Effect of beech extract on hair growth. A: Observation results of hair growth over time (n = 10-14, mean ± SD. ***: P <0.001 (non-paired t-test, vs Vehicle) B: Cultured human hair follicle image after 9 days.

  In the present specification, “non-therapeutic” is a concept that does not include a medical act, that is, a treatment act on the human body by therapy.

  In the present specification, “hair growth inhibition” means an action of suppressing the growth of hair or hair follicles or an action of reducing hair diameter.

In the present specification, “Funabaraso” refers to the perennial plant Cynanchum atratum Bge. Belonging to the genus Gullaceae, and “Funabara extract” means an extract obtained from A. The extract may be an extract from any part of beech grass, for example, whole grass or root, or a combination thereof, but an extract from root is preferred. The part may be subjected to the extraction step as it is, or may be subjected to the extraction step after being pulverized, cut or dried.

  As the above extract, commercially available ones may be used, or various solvent extracts obtained by a conventional method, or diluted solutions thereof, concentrated solutions thereof, dried powders thereof, pastes or activated carbon treatments thereof. There may be. As an example, the beech extract is extracted at room temperature (for example, 4 to 50 ° C.) or under heating (room temperature to solvent boiling point), or extracted using an extractor such as a Soxhlet extractor. Can be obtained.

  As the solvent for extraction, either a polar solvent or a nonpolar solvent can be used. Specific examples of the solvent include water; alcohols such as methanol, ethanol, propanol and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; methyl acetate and ethyl acetate Esters; linear and cyclic ethers such as tetrahydrofuran and diethyl ether; polyethers such as polyethylene glycol; hydrocarbons such as squalane, hexane, cyclohexane and petroleum ether; aromatic hydrocarbons such as toluene; dichloromethane and chloroform And halogenated hydrocarbons such as dichloroethane; and supercritical carbon dioxide; pyridines; organic solvents such as oils and fats, other oils such as wax; and mixtures thereof. Preferable examples include water, alcohols and aqueous solutions thereof, and ethanol is preferable as the alcohols. More preferred solvents are water and an aqueous ethanol solution.

  The mixing ratio (volume ratio) of alcohols and water in the aqueous alcohol solution is preferably 0.001 to 100: 99.999-0, more preferably 5 to 95:95 to 5, and more preferably 20 to 80:80 to 20. Is more preferable, 30 to 70:70 to 30 is still more preferable, and 40 to 60:60 to 40 is still more preferable. In the case of an ethanol aqueous solution, the ethanol concentration is preferably 40 to 60% by volume. As a usage-amount of a solvent, 1-100 mL is preferable with respect to 1 g of beech roots (dry mass conversion), As extraction time, 1 minute-2 months are preferable and 10 minutes-4 weeks are more preferable. The extraction temperature at this time is 0 ° C. to the solvent boiling point, more preferably 10 to 60 ° C., and further preferably 15 to 40 ° C.

  Specific examples of the extraction means for obtaining the extract include solid-liquid extraction, liquid-liquid extraction, immersion, decoction, leaching, reflux extraction, ultrasonic extraction, microwave extraction, and stirring. For example, as a suitable example of immersion, immersion at 15 to 40 ° C. for 1 hour to 4 weeks can be mentioned. Moreover, when shortening extraction time, solid-liquid extraction with stirring is desirable. Examples of suitable conditions for this solid-liquid extraction include stirring at 1000 to 5000 rpm under 10 to 100 ° C. (preferably 20 to 100 ° C.) for 1 to 30 minutes. In order to prevent the oxidation of the extract, a means for extracting under a so-called non-oxidizing atmosphere while removing dissolved oxygen by bubbling degassing or inert gas such as nitrogen gas may be used in combination.

  As will be described later in Examples, the beech extract has an effect of significantly suppressing the elongation of human organ culture hair. Therefore, beech grass or its extract is useful as a hair growth inhibitor. In addition, beech rose or an extract thereof can exert effects such as suppression of hair growth or hair growth, hair removal, and hair removal through the hair growth suppressing action. That is, the beech rose or an extract thereof can be used for hair growth or hair growth inhibition, hair removal, hair removal, or the like. The use can be in humans or non-human animals, or specimens derived therefrom, and can be therapeutic or non-therapeutic.

  Therefore, as one aspect, the present invention provides a hair growth inhibitor containing beech grass or an extract thereof as an active ingredient. In one embodiment, the hair growth inhibitor of the present invention consists essentially of beech grass or its extract. The hair growth inhibitor can be used for hair growth or hair growth inhibition, or for hair removal or hair removal.

  The beech rose or extract thereof or the hair growth inhibitor of the present invention is a composition, medicine, quasi-drug, cosmetic for hair growth inhibition, hair growth or hair growth inhibition, or hair removal or hair removal. It can be used as a food or drink or a raw material thereof, or a feed or a raw material thereof, or can be used for the production thereof. The composition, medicine, quasi-drug, cosmetic, food and drink or raw material thereof, or feed or raw material thereof can be produced or used for human or non-human animals.

  Alternatively, the beech rose or an extract thereof or the hair growth inhibitor of the present invention is a composition, a pharmaceutical, a quasi-drug for hair growth inhibition, hair growth or hair growth inhibition, or hair removal or hair removal, It can be blended as a raw material in cosmetics, food and drink, feed, or food and drink or feed ingredients.

  The pharmaceutical or quasi-drug contains the beech rose or extract thereof or the hair growth inhibitor of the present invention as an active ingredient. The pharmaceutical or quasi drug can be administered in any dosage form. The administration form may be oral administration or parenteral administration such as external preparation. For example, oral dosage forms include solid dosage forms such as tablets, coated tablets, granules, powders, capsules, and liquid dosage forms such as elixirol, syrups and suspensions, and parenteral dosage forms. Examples thereof include injection, infusion, transdermal, transmucosal, nasal, enteral, inhalation, suppository, bolus, and patch.

  The said pharmaceutical and quasi-drug may contain beech rose or extract thereof or the hair growth inhibitor of the present invention alone or in combination with a pharmaceutically acceptable carrier. Examples of such carriers include excipients, coating agents, binders, extenders, disintegrants, surfactants, lubricants, diluents, dispersants, buffers, osmotic pressure adjusting agents, pH adjusting agents, Dispersants, emulsifiers, preservatives, stabilizers, antioxidants, colorants, UV absorbers, moisturizers, thickeners, activity enhancers, anti-inflammatory agents, bactericides, fragrances, flavoring agents, flavoring agents, etc. It is done. Moreover, the said pharmaceutical and quasi-drug may contain another active ingredient and a pharmacological component, as long as the hair growth inhibitory action of a beech rose or its extract is not lost.

  The pharmaceutical or quasi-drug is produced by a conventional method from beech rose or extract thereof or the hair growth inhibitor of the present invention, or in combination with the above carrier and / or other active ingredients and pharmacological ingredients as necessary. can do. The content of beech grass or its extract in the medicine or quasi-drug (in terms of dry matter of the extract) is usually 0.001 to 99.999% by mass and 0.01 to 20% by mass. preferable.

The cosmetics contain beech grass or its extract or the hair growth inhibitor of the present invention as an active ingredient. The cosmetic may contain beech grass or its extract or the hair growth inhibitor of the present invention alone, or may be contained in combination with a carrier acceptable as a cosmetic.
Examples of such carriers include excipients, coating agents, binders, extenders, disintegrants, surfactants, lubricants, diluents, dispersants, buffers, osmotic pressure adjusting agents, pH adjusting agents, Dispersants, emulsifiers, preservatives, stabilizers, antioxidants, colorants, UV absorbers, moisturizers, thickeners, activity enhancers, anti-inflammatory agents, bactericides, fragrances, flavoring agents, flavoring agents, etc. It is done. In addition, the cosmetic, as long as the hair growth inhibitory action of beech rose or its extract is not lost, other active ingredients and cosmetic ingredients, such as humectants, whitening agents, UV protection agents, cell activators, cleaning agents, It may contain keratolytic agents, makeup ingredients (for example, makeup bases, foundations, funny, powder, teak, lipstick, eye makeup, eyebrow, mascara, etc.).
Examples of cosmetic forms include creams, emulsions, lotions, suspensions, foams, gels, powders, packs, sheets, patches, sticks, cakes, and any other form that can be used for cosmetics. As an example, the beech rose or extract thereof or the hair growth inhibitor of the present invention can be used in the form of a hair removal or hair removal lotion, emulsion, cream, foam, gel or the like.

  The cosmetic can be produced by a conventional method from beech rose or extract thereof or the hair growth inhibitor of the present invention, or in combination with the carrier and / or other active ingredients and cosmetic ingredients as necessary. . The content of beech grass or its extract in the cosmetic is usually 0.0001 to 99.999% by mass, and preferably 0.001 to 10% by mass.

  The above-mentioned food or drink or feed, or the raw material thereof contains beech rose or extract thereof or the hair growth inhibitor of the present invention as an active ingredient, such as hair growth inhibition, hair growth or hair growth inhibition, or hair removal or hair loss. It may be a food, a functional food, a food for a sick person, a food for specified health use, a pet food or the like whose function is indicated as necessary, or a raw material thereof. The kind of said food / beverage products is not specifically limited. Examples of the beverage include all beverages such as fruit juice beverages, carbonated beverages, tea beverages, milk beverages, alcoholic beverages, and soft drinks. The form of the food may be any form such as solid, semi-solid, liquid, etc., and may be tablet form, pill form, tablet, capsule form, liquid form, syrup form, powder form, granule form, etc. Also good. For example, as food, breads, noodles, pasta, jelly-like foods, various snacks, cakes, confectionery, ice creams, soups, dairy products, frozen foods, instant foods, other processed foods, seasonings, And supplements. The kind of the feed is not particularly limited and may be a feed for any animal, and the form thereof may be any form as in the case of the food.

  The food or drink or feed, or their raw materials may contain beech rose or extract thereof or the hair growth inhibitor of the present invention alone, or other foods, solvents, softeners, oils, emulsifiers, You may contain combining additives, such as antiseptic | preservative, a fragrance | flavor, a stabilizer, a coloring agent, antioxidant, a moisturizer, and a thickener. The content of beech grass or its extract in the food or drink or feed is usually 0.0001 to 99.999% by mass, preferably 0.001 to 10% by mass.

In addition, the present invention provides a method for inhibiting hair growth, comprising administering beech rose or an extract thereof, or the hair growth inhibitor of the present invention. In this method, the beech rose or extract thereof or the hair growth inhibitor of the present invention is used for subjects that need them for hair growth inhibition, hair growth or hair growth inhibition, or hair removal or hair loss. Administered in an effective amount. Alternatively, the beech rose or extract thereof or the hair growth inhibitor of the present invention is effective for a subject in need thereof for hair growth inhibition, hair growth or hair growth inhibition, or hair removal or hair removal. Ingested. Subjects to be administered or ingested include animals, preferably humans or non-human mammals, more preferably humans.
Alternatively, the subject to be administered or ingested can be an animal-derived tissue, organ, cell, or fraction thereof. The tissue, organ, cell, or fraction thereof is preferably a naturally-derived or biologically or bioengineered tissue, organ, cell, or fraction thereof having hair growth ability. is there.

  Preferred dosages and intakes may vary according to the subject's species, weight, sex, age, condition or other factors. The dose, route, interval, and amount and interval of intake can be determined appropriately by those skilled in the art. For example, when applied to human skin, the dosage is preferably 0.01 to 1000 mg / day per adult, as a beech extract (in terms of dry matter of the extract), and 0.1 to 100 mg / day. Day is more preferred.

  EXAMPLES Hereinafter, an Example is shown and this invention is demonstrated more concretely.

Example 1 Barber Evaluation of Test Substances Using Human Isolated Hair Follicles 40 mL of 50% ethanol aqueous solution was added to 40 g of beech root (Shinwa product) and immersed at room temperature for 27 days. This was filtered to obtain a beech extract. When this beech rose extract was concentrated, the solid content was 4.39 g. The solid content concentration of the extract was 1.33 wt%.
The human scalp sample was disinfected by immersing in 0.1% Hibiten solution for 1 minute, and then washed with PBS. Thereafter, hair follicles were isolated in a William E medium (Invitrogen) using tweezers and a scalpel under a stereomicroscope. The isolated hair follicle was added to William E medium so as to be 2 mM L-Glutamine (Invitrogen), 10 μg / ml Insulin (Invitrogen), 40 ng / ml Hydrocortisone (SIGMA), 1% Antibiotics-antimitotics (Invitrogen) Medium (24 well plate, 300 μl) was cultured under conditions of 37 ° C. and 5% CO ₂ . The beech rose extract was adjusted to a concentration of 1 mg / ml with 50% ethanol and added to the cells to a concentration of 0.1 vol% (final concentration 1 μg / ml). For the control group, the same amount of 50% ethanol solution (Vehicle) was added to the cells.
Human organ cultured hair was photographed under a microscope over time, and the length of the hair follicle was calculated by image analysis based on the scale photographed under the same conditions. Image analysis was performed with NewQube (version 4.0.3, Nexus), and the amount of increase from the initial value was defined as the amount of hair elongation.

  The results are shown in FIG. In the control (Vehicle) group, hair follicle elongation (hair growth) was observed according to the culture time, while in the group added with beech rose, hair follicle elongation was significantly suppressed.

Production Example Using the extract obtained in Example 1 as an active ingredient, lotions, creams, aerosols, packs, foundations, lotions, and gels having the compositions shown below were prepared by conventional methods.

Production Example 1 Hair growth inhibiting lotion A solution A in which the following components A were mixed was prepared. Separately, a solution B in which the following component B was mixed was prepared. The solution B was added to the solution A and stirred and mixed uniformly to obtain a lotion.

(Composition) (Composition: Mass%)
A Polyoxyethylene hydrogenated castor oil 0.8
Ethanol 30.0
B Beech extract (1.0% dry solids)
Sodium dodecyl sulfate 0.12
Dodecylmethylamine oxide 0.18
Isopropyl alcohol 15.0
Benzyl alcohol 15.0
Glycerin 2.0
Purified water balance

Production Example 2 Hair growth inhibiting cream A solution A in which the following components A were mixed was prepared. Separately, a solution B in which the following component B was mixed was prepared. Solution B was added to solution A and stirred and mixed uniformly. After emulsification, the solution was cooled to obtain a cream.

(Composition) (Composition: Mass%)
A Liquid paraffin 10.0
Squalane 7.0
Jojoba oil 3.0
Solid paraffin 3.0
Polyoxyethylene cetyl ether 2.0
Sorbitan sesquioleate 1.0
Potassium hydroxide 0.1
B Beech extract (1.0% dry solids)
Glycerin 3.0
Ethylparaben 0.1
Purified water balance

Production Example 3 Aerosol The following component A was uniformly mixed and placed in a container, and the liquid petroleum gas (propellant) of B was filled into the container by a conventional method to produce an aerosol.

(Composition) (Composition: Mass%)
A Funabara grass extract 1.0 (dry solids)
Cetanol 1.2
Propylene glycol 4.0
Ethanol 8.0
Purified water balance B Liquefied petroleum gas (propellant) 4.0

Production Example 4 Hair growth inhibiting pack agent A pack agent having the following composition was prepared by a conventional method.

(Composition) (Composition: Mass%)
Beech grass extract 3.0 (dry solids)
Polyvinyl alcohol 20.0
Glycerin 5.0
Ethanol 16.0
Fragrance Trace amount Pigment Trace amount Purified water Remaining

Production Example 5 Foundation A foundation having the following composition was prepared by a conventional method.

(Composition) (Composition: Mass%)
Beech extract (1.0% dry solids)
Spherical silica beads 20.0
Silica coated sericite 45.0
Ultrafine titanium oxide 10.0
Yellow iron oxide 3.0
Talc 5.0
Mica 5.0
Bengala 1.0
Gunjo 1.0
Paraben 0.2
Liquid paraffin 4.8
Squalane 4.0

Production Example 6 Hair growth inhibiting lotion A lotion having the following composition was prepared by a conventional method.

(Composition) (Composition: Mass%)
Beech extract (5.0% dry solids)
Glycerin 15.0
Dipropylene glycol 5.0
Purified water balance

Production Example 7 Hair growth inhibiting gel A gel having the following composition was prepared by a conventional method.

(Composition) (Composition: Mass%)
Polyacrylic acid 0.5
Potassium hydroxide 0.15
Gurucam 10.0
Glycerin 10.0
Glycine betaine 3.0
Beech extract (2.0 dry solids)
Succinic acid 1.5
Purified water balance

Claims (2)

  A hair growth inhibitor containing beech rose or an extract thereof as an active ingredient.   The hair growth inhibitor according to claim 1, wherein the extract is a water extract or an aqueous ethanol extract.