JP2020105245A - Biofilm formation preventive agent, and cleaning composition containing said agent - Google Patents
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- 238000004140 cleaning Methods 0.000 title claims abstract description 45
- 230000032770 biofilm formation Effects 0.000 title claims abstract description 44
- 239000000203 mixture Substances 0.000 title claims abstract description 24
- 230000003449 preventive effect Effects 0.000 title abstract 6
- -1 sophorose lipid Chemical class 0.000 claims abstract description 42
- HIWPGCMGAMJNRG-ACCAVRKYSA-N Sophorose Natural products O([C@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HIWPGCMGAMJNRG-ACCAVRKYSA-N 0.000 claims abstract description 39
- HIWPGCMGAMJNRG-UHFFFAOYSA-N beta-sophorose Natural products OC1C(O)C(CO)OC(O)C1OC1C(O)C(O)C(O)C(CO)O1 HIWPGCMGAMJNRG-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000004094 surface-active agent Substances 0.000 claims abstract description 31
- 150000008051 alkyl sulfates Chemical class 0.000 claims abstract description 23
- 239000003112 inhibitor Substances 0.000 claims description 23
- 150000002596 lactones Chemical class 0.000 claims description 6
- 230000000813 microbial effect Effects 0.000 abstract description 9
- 230000002265 prevention Effects 0.000 abstract description 9
- 230000006378 damage Effects 0.000 abstract description 8
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 abstract description 8
- 230000007613 environmental effect Effects 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 3
- 239000003899 bactericide agent Substances 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 27
- 239000003599 detergent Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 14
- 238000004851 dishwashing Methods 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 9
- 230000015572 biosynthetic process Effects 0.000 description 8
- 230000003405 preventing effect Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000010411 cooking Methods 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 4
- 239000000645 desinfectant Substances 0.000 description 4
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
- 239000000194 fatty acid Substances 0.000 description 4
- 229930195729 fatty acid Natural products 0.000 description 4
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 238000012790 confirmation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 235000019645 odor Nutrition 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- PZDOWFGHCNHPQD-VNNZMYODSA-N sophorose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](C=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PZDOWFGHCNHPQD-VNNZMYODSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 150000003973 alkyl amines Chemical class 0.000 description 2
- 229960003237 betaine Drugs 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 239000012459 cleaning agent Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000003002 pH adjusting agent Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
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- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- BTBJBAZGXNKLQC-UHFFFAOYSA-N ammonium lauryl sulfate Chemical compound [NH4+].CCCCCCCCCCCCOS([O-])(=O)=O BTBJBAZGXNKLQC-UHFFFAOYSA-N 0.000 description 1
- 229940063953 ammonium lauryl sulfate Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229910001919 chlorite Inorganic materials 0.000 description 1
- 229910052619 chlorite group Inorganic materials 0.000 description 1
- QBWCMBCROVPCKQ-UHFFFAOYSA-N chlorous acid Chemical compound OCl=O QBWCMBCROVPCKQ-UHFFFAOYSA-N 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
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- 239000001963 growth medium Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
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- 238000004519 manufacturing process Methods 0.000 description 1
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- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- ONQDVAFWWYYXHM-UHFFFAOYSA-M potassium lauryl sulfate Chemical compound [K+].CCCCCCCCCCCCOS([O-])(=O)=O ONQDVAFWWYYXHM-UHFFFAOYSA-M 0.000 description 1
- 229940116985 potassium lauryl sulfate Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229940080236 sodium cetyl sulfate Drugs 0.000 description 1
- GGHPAKFFUZUEKL-UHFFFAOYSA-M sodium;hexadecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCCCOS([O-])(=O)=O GGHPAKFFUZUEKL-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 150000003396 sophoroses Chemical class 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
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- 238000010998 test method Methods 0.000 description 1
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Landscapes
- Detergent Compositions (AREA)
Abstract
Description
本発明は、ソホロースリピッドと、アルキル硫酸塩系界面活性剤とを含有するバイオフィルム形成防止剤、及び、該剤を含有する洗浄用組成物に関する。 The present invention relates to a biofilm formation inhibitor containing sophorose lipid and an alkyl sulfate-based surfactant, and a cleaning composition containing the agent.
バイオフィルムは、固体の表面に緑膿菌等の微生物と微生物が産生する菌体外多糖等の生産物が集まって形成された構造体である。バイオフィルムにおいて、微生物は、自己が産生する菌体外多糖等の生産物からなる細胞外マトリックスに埋め込まれている。バイオフィルムは、水分があり微生物が増殖し易い場所であれば、どんな場所でも生じ得るので、様々な場所で微生物による害を引き起こす。例えば、家庭では、台所のシンク、調理場、洗面台、洗濯槽、浴室、便器、排水溝、排水管等の水まわりでバイオフィルムが形成されてヌメリや悪臭の原因となり、ひどい場合には、排水溝や排水管の詰まりの原因となる。また、食器、調理器具、洗浄道具等の水まわりで使用するものにバイオフィルムが形成されると、衛生が損なわれ食中毒等の微生物汚染の原因となる。水を使用する工場プラントでは、形成されるバイオフィルムが製品の微生物汚染、金属腐食による設備劣化の原因となる。病院では、医療器具に形成されるバイオフィルムが院内感染の原因となる。 A biofilm is a structure formed by collecting microorganisms such as Pseudomonas aeruginosa and products such as extracellular polysaccharides produced by the microorganisms on the surface of a solid. In a biofilm, microorganisms are embedded in an extracellular matrix composed of products such as extracellular polysaccharides produced by themselves. Biofilms can cause microbial harm in a variety of locations, as they can occur in any location where water is present and microorganisms are prone to growth. For example, at home, a biofilm is formed around water in kitchen sinks, kitchens, washbasins, washing tubs, bathrooms, toilets, drains, drains, etc., causing slime and malodor, and in severe cases, It may cause clogging of drains and drains. In addition, if a biofilm is formed on things used around water such as tableware, cooking utensils, and washing tools, hygiene is impaired, which causes microbial contamination such as food poisoning. In factory plants that use water, the biofilms formed cause microbial contamination of products and deterioration of equipment due to metal corrosion. In hospitals, biofilms formed on medical devices cause nosocomial infections.
このような微生物による害を引き起こすバイオフィルムを除去するために、従来は、次亜塩素酸塩等の殺菌剤を使用していたが、効果は不十分であった。その理由は、前述のように、バイオフィルムにおいては、微生物は、自己が産生する菌体外多糖等の生産物からなる細胞外マトリックスに埋め込まれていて、殺菌剤から保護されるためである。また、次亜塩素酸塩は、皮膚や粘膜につかないように注意する必要がある等、人体に安全とは言い難く、環境に対する負荷も高い。 In order to remove the biofilm causing the damage by such microorganisms, a bactericide such as hypochlorite has been conventionally used, but the effect was insufficient. The reason is that, as described above, in the biofilm, the microorganism is embedded in the extracellular matrix made of a product such as extracellular polysaccharide produced by itself and protected from the bactericide. Further, hypochlorite is not safe for the human body because it is necessary to take care not to get it on the skin and mucous membranes, and the burden on the environment is high.
家庭の水まわり、水まわりで使用するもの、水を使用する工場プラントの衛生的な使用のためには、上述したバイオフィルムの除去に加えて、洗浄が必要となるが、従来、両者は次亜塩素酸塩等の殺菌剤と、洗浄剤とにより別々に行われていた。しかしながら、両者を別々に行うことは、手間がかかり不便であると同時に、手入れの忘却を招来し、バイオフィルムによる微生物害を招く原因となる。従って、両者を同時に行えると便利であると同時に、手入れの忘却を防ぎ、バイオフィルムによる微生物害を未然に防止し得る。特に、食器、調理器具、洗浄道具等の家庭の水まわりで日々洗浄するものの洗浄剤でバイオフィルム形成防止も同時に行えると、別途殺菌剤で水まわりや水まわりで使用するもののバイオフィルム除去を行う必要がなくなり、大変便利であると同時に、手入れの忘却を防ぎ、水まわりのヌメリや悪臭、排水溝や排水管の詰まりを未然に防止し得ると共に、水まわりで使用するものの洗浄後の衛生も保ち得る。しかしながら、このようにバイオフィルム除去と洗浄とを同時に行うという考えは現状では見られない。 For sanitary use of household water, those used around water, and plant plants that use water, in addition to the removal of the biofilm described above, cleaning is required. The disinfectant such as chlorite and the cleaning agent are separately used. However, performing both separately is not only time-consuming and inconvenient, but also causes forgetting of maintenance and causes microbial damage due to the biofilm. Therefore, it is convenient if both can be performed at the same time, and at the same time, forgetting of care can be prevented and microbial damage due to biofilm can be prevented. In particular, if you can prevent biofilm formation at the same time with a cleaning agent for household dishes such as tableware, cooking utensils, cleaning tools, etc., which is used daily, you can also use a disinfectant to remove biofilms around water or those that are used around water. It is unnecessary and very convenient, while at the same time preventing forgetting of care, preventing slime and foul odor around water, clogging of drains and drain pipes, and hygiene after washing things used around water. Can be kept. However, the idea of simultaneously performing biofilm removal and washing in this way is not found at present.
ソホロースリピッドについては、人体に安全に使用し得、また、生分解性に優れ環境負荷も低い洗浄剤として知られている（特許文献１、２）。しかしながら、ソホロースリピッドがバイオフィルム形成防止作用を有することは知られていない。 Sophorose lipid is known as a detergent that can be safely used by the human body and is excellent in biodegradability and has a low environmental load (Patent Documents 1 and 2). However, it is not known that sophorose lipid has a biofilm formation-preventing action.
アルキル硫酸塩系界面活性剤も洗浄剤として汎用されているが、バイオフィルム形成防止作用を有することは知られていない。 Alkyl sulfate-based surfactants are also widely used as detergents, but they are not known to have a biofilm formation-preventing action.
本発明は、従来、バイオフィルムの除去に使用されている次亜塩素酸塩等の殺菌剤と異なり、十分なバイオフィルム形成防止作用を有すると共に、人体に安全で環境負荷も低い、バイオフィルム形成防止剤を提供することを目的とする。 The present invention, unlike biocides such as hypochlorite, which has been conventionally used for removing biofilms, has a sufficient biofilm formation-preventing action, is safe for humans, and has a low environmental load. The purpose is to provide an inhibitor.
また、本発明は、バイオフィルム形成防止と洗浄とを同時に行えることから、便利であると同時に、手入れの忘却を防ぎ、バイオフィルムによる微生物害を未然に防止し得る、バイオフィルム形成防止剤含有洗浄用組成物を提供することを目的とする。 In addition, the present invention is convenient because at the same time, it is possible to prevent biofilm formation and cleaning, and at the same time, prevent forgetting of care and prevent microbial damage due to biofilm in advance. An object is to provide a composition for use.
発明者らは、十分なバイオフィルム形成防止作用を有すると共に、人体に安全で環境負荷も低い物質を種々探索したところ、ソホロースリピッドがこれらの要件を全て満たすことを見出した。また、ソホロースリピッドは洗浄作用も有することから、これによりバイオフィルム形成防止と洗浄とを同時に行えることも見出した。 The inventors of the present invention have searched for various substances that have a sufficient biofilm formation-preventing action and are safe for humans and have a low environmental load, and have found that sophorose lipid satisfies all of these requirements. Further, since sophorose lipid also has a cleaning action, it was found that biofilm formation prevention and cleaning can be performed at the same time.
さらに、ソホロースリピッドにアルキル硫酸塩系界面活性剤を加えるとバイオフィルム形成防止作用が相乗的に向上すること、アルキル硫酸塩系界面活性剤も洗浄作用を有することから、これを加えた組成物についてもバイオフィルム形成防止と洗浄とを同時に行えることも見出した。 Furthermore, when an alkyl sulfate-based surfactant is added to sophorose lipid, the biofilm formation-preventing action is synergistically improved, and the alkyl sulfate-based surfactant also has a cleaning action. It was also found that biofilm formation prevention and cleaning can be performed simultaneously.
すなわち、本発明は、ソホロースリピッドと、アルキル硫酸塩系界面活性剤とを含有する、バイオフィルム形成防止剤を提供するものである。 That is, the present invention provides a biofilm formation inhibitor containing sophorose lipid and an alkyl sulfate-based surfactant.
この発明の好ましい態様によれば、上記のソホロースリピッドの２０重量％以上８０重量％以下がラクトン型のソホロースリピッドである、上記のバイオフィルム形成防止剤が提供される。 According to a preferred embodiment of the present invention, there is provided the biofilm formation inhibitor, wherein 20% by weight or more and 80% by weight or less of the sophorose lipid is a lactone type sophorose lipid.
また、本発明は、上記のバイオフィルム形成防止剤を含有する、洗浄用組成物を提供するものである。 The present invention also provides a cleaning composition containing the biofilm formation inhibitor.
この発明の好ましい態様によれば、上記のソホロースリピッドの含有量が０．０５〜１０．０重量％である、上記の洗浄用組成物；上記のアルキル硫酸塩系界面活性剤の含有量が０．１〜５．０重量％である、上記の洗浄用組成物が提供される。 According to a preferred embodiment of the present invention, the content of the above-mentioned sophorose lipid is 0.05 to 10.0% by weight, and the content of the above-mentioned alkyl sulfate-based surfactant is A cleaning composition as described above is provided which is 0.1 to 5.0% by weight.
本発明のバイオフィルム形成防止剤は、バイオフィルムの形成を十分に防止し得ると共に、人体に安全で環境負荷も低い。 The biofilm formation inhibitor of the present invention can sufficiently prevent the formation of biofilms, is safe for the human body, and has a low environmental load.
また、本発明のバイオフィルム形成防止剤は、洗浄作用も有しており、洗浄用組成物に含有させるとバイオフィルム形成防止と洗浄とを同時に行えることから、便利であると同時に、手入れの忘却を防ぎ、バイオフィルムによる微生物害を未然に防止し得る。 Further, the biofilm formation inhibitor of the present invention also has a cleaning action, and when contained in a cleaning composition, it can simultaneously prevent biofilm formation and cleaning, which is convenient and forgets to care. It is possible to prevent the microbial damage caused by the biofilm.
特に、食器、調理器具、洗浄道具等の家庭の水まわりで日々洗浄するものの洗浄用組成物に含有させると、バイオフィルム形成防止と洗浄とを同時に行えることから、別途殺菌剤で水まわりや水まわりで使用するもののバイオフィルム除去を行う必要がなくなり、大変便利であると同時に、手入れの忘却を防ぎ、水まわりのヌメリや悪臭、排水溝や排水管の詰まりを未然に防止し得ると共に、水まわりで使用するものの洗浄後の衛生も保ち得る。 In particular, when it is contained in a cleaning composition for daily cleaning in household water such as tableware, cooking utensils, cleaning tools, etc., biofilm formation prevention and cleaning can be performed at the same time. It is very convenient because it eliminates the need to remove the biofilm used in the surroundings, while at the same time preventing forgetting of maintenance, preventing slime and odor around water, clogging of drains and drainage pipes, and water. It can keep the hygiene of things used around it after cleaning.
本実施形態において、ソホロースリピッドは、バイオフィルム形成防止作用を奏する。また、人体に安全で環境負荷も低い。さらに、洗浄作用を有することから、バイオフィルム形成防止剤含有洗浄用組成物にも好適に配合される。 In the present embodiment, sophorose lipid has an action of preventing biofilm formation. In addition, it is safe for the human body and has a low environmental impact. Further, since it has a cleaning action, it is also suitably blended with a cleaning composition containing a biofilm formation inhibitor.
ソホロースリピッドの構造を下記に示す。ソホロースリピッドは、グルコース２量体のソホロースとヒドロキシ脂肪酸とが結合した糖脂質である。ソホロースリピッドは、ヒドロキシ脂肪酸のカルボキシル基が遊離した酸型と、このカルボキシ基が分子内のソホロースのヒドロキシ基に結合したラクトン型との混合物である。ソホロースのヒドロキシ基の一部はアセチル化されていてもよい。 The structure of sophorose lipid is shown below. Sophorose lipid is a glycolipid in which glucose dimer sophorose and hydroxy fatty acid are bound. Sophorose lipid is a mixture of an acid form in which a carboxyl group of hydroxy fatty acid is released and a lactone form in which this carboxy group is bonded to the hydroxy group of sophorose in the molecule. Part of the hydroxy groups of sophorose may be acetylated.
本実施形態において、好ましくは、ソホロースリピッドの２０重量％以上８０重量％以下がラクトン型のソホロースリピッドである。より好ましくは、ソホロースリピッドの４０重量％以上８０重量％以下、さらに好ましくは、ソホロースリピッドの６０重量％以上８０重量％以下が、ラクトン型のソホロースリピッドである。ラクトン型のソホロースリピッドを上記の範囲内にすることにより、バイオフィルム形成防止作用が向上するのみならず、液性の安定性も増し、濁りや分離が生じにくくなるため、好ましい。 In the present embodiment, preferably 20% by weight or more and 80% by weight or less of the sophorose lipid is a lactone type sophorose lipid. More preferably, 40% by weight or more and 80% by weight or less of sophorose lipid, and even more preferably 60% by weight or more and 80% by weight or less of sophorose lipid are lactone type sophorose lipids. When the lactone type sophorose lipid is in the above range, not only the biofilm formation-preventing action is improved, but also the liquid stability is increased and turbidity or separation is less likely to occur, which is preferable.
本実施形態に係るバイオフィルム形成防止剤、及び、バイオフィルム形成防止剤含有洗浄用組成物におけるソホロースリピッドの含有量は、バイオフィルム形成防止作用、及び、洗浄力の観点から、好ましくは、０．０５〜１０．０重量％であり、さらに好ましくは、０．０５〜５重量％である。 Biofilm formation inhibitor according to the present embodiment, and the content of sophorose lipid in the biofilm formation inhibitor-containing cleaning composition is preferably 0 from the viewpoint of biofilm formation prevention action and detergency. 0.05 to 10.0% by weight, and more preferably 0.05 to 5% by weight.
本実施形態において、アルキル硫酸塩系界面活性剤は、ソホロースリピッドのバイオフィルム形成防止作用を相乗的に向上させることができる。また、洗浄作用を有することから、バイオフィルム形成防止剤含有洗浄用組成物にも好適に配合される。 In the present embodiment, the alkyl sulfate-based surfactant can synergistically improve the biofilm formation-preventing action of sophorose lipid. Further, since it has a cleaning action, it is also suitably blended in a cleaning composition containing a biofilm formation inhibitor.
アルキル硫酸塩系界面活性剤としては、例えば、ラウリル硫酸ナトリウム、ラウリル硫酸アンモニウム、ラウリル硫酸カリウム、セチル硫酸ナトリウム等が挙げられる。好ましい例としては、ラウリル硫酸ナトリウムが挙げられる。 Examples of the alkyl sulfate-based surfactants include sodium lauryl sulfate, ammonium lauryl sulfate, potassium lauryl sulfate, sodium cetyl sulfate and the like. A preferred example is sodium lauryl sulfate.
本実施形態に係るバイオフィルム形成防止剤、及び、バイオフィルム形成防止剤含有洗浄用組成物におけるアルキル硫酸塩系界面活性剤の含有量は、ソホロースリピッドのバイオフィルム形成防止作用を向上させる観点、及び、洗浄力の観点から、好ましくは、０．１〜５．０重量％である。 Biofilm formation inhibitor according to the present embodiment, and the content of the alkyl sulfate-based surfactant in the biofilm formation inhibitor-containing cleaning composition, from the viewpoint of improving the biofilm formation preventing action of sophorose lipid, And, from the viewpoint of detergency, it is preferably 0.1 to 5.0% by weight.
本実施形態に係るバイオフィルム形成防止剤、及び、バイオフィルム形成防止剤含有洗浄用組成物は、ソホロースリピッド、アルキル硫酸塩系界面活性剤、以外の成分として、本発明のバイオフィルム形成防止作用、洗浄作用を妨げない限りにおいて、バイオフィルム形成防止剤、及び、洗浄用組成物に一般的に含有される成分を含有し得る。 Biofilm formation inhibitor according to the present embodiment, and biofilm formation inhibitor-containing cleaning composition, sophorose lipid, alkyl sulfate-based surfactant, as a component other than the biofilm formation inhibitory effect of the present invention As long as it does not interfere with the cleaning action, it may contain a biofilm formation inhibitor and components generally contained in cleaning compositions.
バイオフィルム形成防止剤含有洗浄用組成物に含有し得る成分として、洗浄用組成物に一般的に含有される成分、例えば、ベタイン系界面活性剤、アルキルエーテル硫酸エステル塩系界面活性剤、アルキルアミンオキシド系界面活性剤、脂肪酸塩系界面活性剤、等の界面活性剤、エタノール等の可溶化剤、粘度調節剤、ｐＨ調節剤、除菌剤、防腐剤、香料、水等の溶剤等が挙げられる。 As a component that may be contained in the biofilm formation inhibitor-containing cleaning composition, a component generally contained in the cleaning composition, for example, betaine-based surfactant, alkyl ether sulfate ester-based surfactant, alkylamine Examples include oxide surfactants, fatty acid salt surfactants and other surfactants, ethanol and other solubilizers, viscosity modifiers, pH modifiers, disinfectants, preservatives, fragrances, water and other solvents. To be
各成分の含有量としては、ベタイン系界面活性剤は０．１〜５．０重量％、アルキルエーテル硫酸エステル塩系界面活性剤、アルキルアミンオキシド系界面活性剤は５．０〜２０．０重量％、エタノール等の可溶化剤、粘度調節剤は１．０〜５．０重量％、ｐＨ調節剤は０．５〜１．０重量％、脂肪酸塩系界面活性剤、除菌剤、防腐剤、香料は０．５重量％以下が好ましく、残部は水等の溶剤である。 The content of each component is 0.1 to 5.0% by weight of betaine surfactant, 5.0 to 20.0% by weight of alkyl ether sulfate ester surfactant and alkylamine oxide surfactant. %, solubilizing agents such as ethanol, viscosity adjusting agents 1.0 to 5.0% by weight, pH adjusting agents 0.5 to 1.0% by weight, fatty acid salt surfactants, disinfectants, preservatives The fragrance is preferably 0.5% by weight or less, and the balance is a solvent such as water.
本実施形態に係るバイオフィルム形成防止剤は、任意の場所に生じるバイオフィルムの形成防止に使用し得るが、好ましくは、台所のシンク、調理場、洗面台、洗濯槽、浴室、便器、排水溝、排水管等の家庭の水まわり、食器、調理器具、洗浄道具等の家庭の水まわりで使用するもの、に形成されるバイオフィルムの形成防止に使用し得る。 The biofilm formation inhibitor according to the present embodiment can be used to prevent the formation of biofilm that occurs at any place, but preferably it is a kitchen sink, a kitchen, a wash basin, a washing tub, a bathroom, a toilet bowl, a drainage gutter. , It can be used to prevent the formation of biofilms formed around household water such as drainage pipes, those used around household water such as tableware, cooking utensils, and cleaning tools.
本実施形態に係るバイオフィルム形成防止剤を含有する洗浄用組成物は、バイオフィルム形成防止及び洗浄が必要な任意の場面で使用し得るが、好ましくは、台所のシンク、調理場、洗面台、洗濯槽、浴室、便器、排水溝、排水管等の家庭の水まわり、食器、調理器具、洗浄道具等の家庭の水まわりで使用するもの、に対して、バイオフィルム形成防止及び洗浄のために使用し得る。係る実施形態により、バイオフィルム形成防止と洗浄とを同時に行えることから、便利であると同時に、手入れの忘却を防ぎ、バイオフィルムによる微生物害を未然に防止し得る。 The cleaning composition containing the biofilm formation inhibitor according to the present embodiment can be used in any scene where biofilm formation prevention and cleaning are required, but preferably a kitchen sink, a kitchen, a wash basin, For preventing biofilm formation and cleaning of household tubs such as washing tubs, bathrooms, toilet bowls, drains, drainage pipes, etc. that are used around premises such as tableware, cooking utensils, cleaning tools, etc. Can be used. According to such an embodiment, biofilm formation can be prevented and cleaning can be performed at the same time, which is convenient, and at the same time, it is possible to prevent forgetting of care and prevent microbial damage due to biofilm.
本実施形態に係るバイオフィルム形成防止剤を含有する洗浄用組成物は、さらに好ましくは、食器、調理器具、洗浄道具等の家庭の水まわりで日々洗浄するものに対して使用すると、バイオフィルム形成防止と洗浄とを同時に行えることから、別途殺菌剤で水まわりや水まわりで使用するもののバイオフィルム除去を行う必要がなくなり、大変便利であると同時に、手入れの忘却を防ぎ、水まわりのヌメリや悪臭、排水溝や排水管の詰まりを未然に防止し得ると共に、水まわりで使用するものの洗浄後の衛生も保ち得る。 The cleaning composition containing the biofilm formation inhibitor according to the present embodiment, more preferably, when used for things that are washed daily around household water such as tableware, cooking utensils, and cleaning tools, biofilm formation Since it is possible to prevent and wash at the same time, it is not necessary to remove the biofilm of water and a separate sterilizing agent that is used around water. It is possible to prevent bad odors, clogging of drains and drainage pipes, and also to maintain hygiene of products used around water after cleaning.
１．製造例
（１）食器用洗剤の調製
下記表１に示す配合で、ソホロースリピッド及びアルキル硫酸塩系界面活性剤を配合する食器用洗剤（実施例１）を調製した。また、比較例として、ソホロースリピッドを配合しアルキル硫酸塩系界面活性剤は配合しない食器用洗剤（比較例１）、及び、アルキル硫酸塩系界面活性剤を配合しソホロースリピッドは配合しない食器用洗剤（比較例２）を調製した。さらに、除菌効果を謳っている各種市販品食器用洗剤（比較例３〜５）の成分表を表２〜表４に示す。
1. Production Example (1) Preparation of Dishwashing Detergent A dishwashing detergent (Example 1) containing sophorose lipid and an alkyl sulfate-based surfactant was prepared according to the formulation shown in Table 1 below. In addition, as comparative examples, a dishwashing detergent containing sophorose lipid and not containing an alkyl sulfate-based surfactant (Comparative Example 1), and tableware containing an alkyl sulfate surfactant but not containing sophorose-lipid A detergent (Comparative Example 2) was prepared. Further, Tables 2 to 4 show the ingredients of various commercially available dishwashing detergents (Comparative Examples 3 to 5) which are said to have a sterilizing effect.
２．バイオフィルム形成防止作用の確認試験
(１)試験の概要
ソホロースリピッド及びアルキル硫酸塩系界面活性剤を配合する食器用洗剤（実施例１）、ソホロースリピッドを配合しアルキル硫酸塩系界面活性剤は配合しない食器用洗剤（比較例１）、アルキル硫酸塩系界面活性剤を配合しソホロースリピッドは配合しない食器用洗剤（比較例２）、及び、各種市販品食器用洗剤（比較例３〜５）による緑膿菌バイオフィルム（BF）に対する形成防止作用について、96穴プレートを用いたCV染色法による評価で検証を行った。
2. Confirmation test of biofilm formation prevention action
(1) Outline of test Dishwashing detergent containing sophorose lipid and an alkylsulfate surfactant (Example 1), dishwashing detergent containing sophorose lipid and not containing an alkylsulfate surfactant (comparison) Pseudomonas aeruginosa biofilm prepared from Example 1), a dishwashing detergent containing an alkyl sulfate-based surfactant and not containing sophorose lipid (Comparative Example 2), and various commercial dishwashing detergents (Comparative Examples 3 to 5) The anti-formation effect on (BF) was verified by evaluation by the CV staining method using a 96-well plate.
(２)試験菌
Pseudomonas aeruginosa NBRC 13275 （緑膿菌）
(2) Test bacteria
Pseudomonas aeruginosa NBRC 13275 (Pseudomonas aeruginosa)
(３)試験方法
(３−１)試験品共存下でのBF形成の確認試験
(a)試験品共存下でのBF 形成
被検菌株は凍結保存品を復元し、SCD 寒天培地による24 時間の前培養後に滅菌生理食塩水で106〜107cfu/mLオーダーとなるように調製した。これを2.0×106cfu/mLオーダーとなるように2倍濃度のトリプトソイブロス（TSB）で希釈し、菌添加済みTSBとした。終濃度の倍濃度となるように滅菌精製水で希釈した各試験品を菌添加済みTSBを用いて2倍希釈し、96穴マイクロプレートに1 種類に付きプレートの縦列（8穴）に200μlずつ添加した。96 穴マイクロプレートにフタをして35℃の恒温器内で24 時間の培養を行い、BF 形成させた。
(3) Test method
(3-1) Confirmation test of BF formation in the coexistence of test products
(a) BF formation in the coexistence of test products
The test strain was reconstituted from a cryopreserved product, and after preculturing on SCD agar medium for 24 hours, it was prepared with sterile physiological saline to a concentration of 10 6 to 10 7 cfu/mL. This was diluted with a 2-fold concentration of tryptosoy broth (TSB) so that the order was 2.0×10 6 cfu/mL, and the cells were added with bacteria to obtain TSB. Each test product diluted to double the final concentration with sterile purified water is diluted 2-fold with bacteria-supplemented TSB, and each 96 μl microplate is provided with 200 μl per plate column (8 wells). Was added. The 96-well microplate was covered with a lid and incubated in a thermostat at 35°C for 24 hours to form BF.
(b)CV染色法によるBFの染色・抽出
BF形成プレートについて、各穴から培地および増殖菌の浮遊部分を吸引装置により回収し、更に滅菌精製水を加えて吸引することで洗浄し、これを2回繰り返した後、室温で十分に乾燥させた。乾燥後のプレートについて、各穴に0.1%（w/v）クリスタルバイオレット（CV）水溶液220μlを添加して、形成したBFを染色した。15 分静置後に吸引して除去し、滅菌精製水で2回洗浄して十分に乾燥させた後、99.5％エタノールを250μl添加して残留したCVを抽出し、各穴から新たなマイクロプレートに抽出液を200μlずつ回収し、マイクロプレートリーダーによる測定に供した。
(b) Staining and extraction of BF by CV staining method
For the BF forming plate, the floating portion of the culture medium and the proliferating bacteria was collected from each hole with a suction device, further washed by adding sterile purified water and sucking, and this was repeated twice, and then dried sufficiently at room temperature. It was On the dried plate, 220 μl of 0.1% (w/v) crystal violet (CV) aqueous solution was added to each well to stain the formed BF. After allowing to stand for 15 minutes, remove by aspiration, wash twice with sterilized purified water, and dry thoroughly, then add 250 μl of 99.5% ethanol to extract the residual CV and transfer it to a new microplate from each well. 200 μl of each extract was collected and subjected to measurement by a microplate reader.
(４)試験結果
(４−１)試験品共存下でのBF形成の確認試験
96穴マイクロプレートに形成したBFに対する各試験品作用後のCV染色試験結果を表５及び表６に示す。
(4) Test results
(4-1) Confirmation test of BF formation in the coexistence of test products
Table 5 and Table 6 show the results of the CV staining test after the action of each test product on the BF formed on the 96-well microplate.
なお、BF形成防止作用については滅菌精製水処理区での値と各試験品での値の比較からBF形成防止率（％）={1-(検体の吸光度平均/滅菌精製水の吸光度平均)}×100を算出して評価した。 Regarding the BF formation prevention effect, the BF formation prevention rate (%) = {1-(average absorbance of the sample/average absorbance of the sterilized purified water) from the comparison of the values in the sterilized purified water treatment area and the values for each test product. }×100 was calculated and evaluated.
ソホロースリピッド及びアルキル硫酸塩系界面活性剤を配合する食器用洗剤（実施例１）がバイオフィルム形成防止作用を有することが確認された。アルキル硫酸塩系界面活性剤を配合しソホロースリピッドは配合しない食器用洗剤（比較例２）ではバイオフィルム形成防止作用が認められないにもかかわらず、ソホロースリピッドを配合しアルキル硫酸塩系界面活性剤は配合しない食器用洗剤（比較例１）にアルキル硫酸塩系界面活性剤を加えるとバイオフィルム形成防止作用が向上することから、両者の併用による効果の向上は相乗的であることが確認された。なお、除菌効果を謳っている市販品食器用洗剤（比較例３〜５）はバイオフィルム形成防止作用が弱いか或いはみられなかった。 It was confirmed that the dishwashing detergent containing the sophorose lipid and the alkyl sulfate-based surfactant (Example 1) has a biofilm formation-preventing action. Although the dishwashing detergent (Comparative Example 2) containing an alkyl sulfate-based surfactant and not containing sophorose lipid does not have a biofilm formation-preventing action, it contains an alkyl sulfate-based interface with sophorose lipid. It was confirmed that the addition of the alkyl sulfate-based surfactant to the dishwashing detergent (Comparative Example 1) in which the activator is not mixed improves the biofilm formation-preventing action, so that the combined use of the two is synergistic. Was done. In addition, the commercially available dishwashing detergents (Comparative Examples 3 to 5), which are said to have a sterilizing effect, have weak or no biofilm formation-preventing action.
Claims (5)
The cleaning composition according to claim 3 or 4, wherein the content of the alkyl sulfate-based surfactant is 0.1 to 5.0% by weight.
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