JP2018154607A - Osteoblast-activating composition, and bone-type alkali phosphatase promoting composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an osteoblast-activating composition for activating osteoblasts.SOLUTION: An osteoblast-activating composition contains lemon juice as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a composition for activating osteoblasts and a composition for promoting bone-type alkaline phosphatase.

  Currently, various bone diseases, such as osteoporosis and fractures, are increasing and are particularly problematic in an aging society. Currently, research and development for the prevention and treatment of various bone diseases is underway. While research and development for the prevention and treatment of various bone diseases are underway, research on bone and calcium metabolism is now progressing rapidly. Among bone diseases, for example, osteoporosis is considered to be caused by, for example, insufficient intake of calcium, decreased calcium ion absorption ability, and postmenopausal hormone imbalance. Therefore, enhancing calcium ion absorption is considered to be one of the effective means for preventing or treating bone diseases.

  However, it is known that bone density does not rise easily even if only calcium is continuously ingested. For example, Non-Patent Documents 1 to 3 disclose a test in which bone density was measured after ingesting 600 to 800 mg / day of calcium in the form of a tablet or the like for several months to one year. It is disclosed that none of these tests was able to significantly increase bone density.

Journal of Nutrition (2014) Vol. 144, pp. 297-304 British Journal of Nutrition (2006) 96, 1140-1148 International Journal Medical Sciences (2011) Volume 8, Pages 180-191

  Incidentally, osteoblasts are known as one of the cells responsible for bone formation. When preventing or treating bone diseases such as osteoporosis, it is considered important to control osteoblasts. Bone-type alkaline phosphatase is also known as a bone formation marker that is an indicator of bone formation by osteoblasts.

  An object of the present invention is to provide a composition for activating osteoblasts that activates osteoblasts and a composition for promoting bone alkaline phosphatase that promotes bone alkaline phosphatase.

The composition for activating osteoblasts for achieving the above object comprises lemon juice as an active ingredient.
The composition for activating osteoblasts preferably contains the lemon juice in an amount equivalent to 10 ml of lemon juice in 100% conversion.

  The composition for promoting bone-type alkaline phosphatase for achieving the above object comprises lemon juice as an active ingredient.

  According to the composition for activating osteoblasts of the present invention, osteoblasts can be activated. Moreover, according to the composition for promoting bone alkaline phosphatase of the present invention, bone alkaline phosphatase can be promoted.

Hereinafter, an embodiment of the present invention will be described.
The composition of the present embodiment (hereinafter referred to as the present composition) contains lemon juice as an active ingredient.

  Lemon juice is an extract of lemon fruit, and is obtained, for example, by crushing lemon fruit, squeezing or squeezing it, and removing skin, seeds, and the like. Normally, lemon juice is divided into turbid juice, semi-clear juice, and transparent juice according to the amount of pulp contained, and there are concentrated juice, concentrated reduced juice obtained by reducing the concentrated juice, and straight juice. The lemon juice contained in the present composition may be any of the above juices. Moreover, only 1 type may be used for lemon fruit juice among said fruit juice, and it may use it in combination of 2 or more type.

Next, the effect | action of this composition is demonstrated.
Ingestion of the composition activates osteoblasts. Moreover, bone-type alkaline phosphatase increases by ingesting this composition. Therefore, the present composition comprises an osteoblast activation composition for the purpose of demonstrating osteoblast activation and a bone-type alkaline phosphatase promotion composition for the purpose of demonstrating bone-type alkaline phosphatase. It can be applied as a thing. And the composition for osteoblast activation which consists of this composition, and the composition for bone-type alkaline phosphatase promotion can each be applied to each field | areas, such as food-drinks, a pharmaceutical, and a quasi-drug.

  Examples of the food and drink include various beverages (beverages or vegetable juice-containing beverages, soft drinks, mineral beverages, sports drinks, tea beverages, coffee, carbonated beverages, milk products such as milk and yogurt), and jelly-like foods. (Jelly, agar, jelly-like beverages, etc.), capsules (soft capsules, hard capsules), and various confectionery. Foods and drinks include gelling agents such as pectin and carrageenan, sugars and sweeteners such as glucose, sucrose, fructose, lactose, stevia, aspartame and sugar alcohol, food additives such as fragrances, vegetable oils and animal fats and oils Oils and fats such as can be contained as appropriate. In addition, the use of food and drink is not particularly limited, and can be applied as so-called general foods, health foods, functional foods, dietary supplements, supplements, foods for specified health use, functional indication foods, foods for the sick. it can.

  The administration method in the case of using as said pharmaceutical is not specifically limited. Specific administration methods include administration by taking (oral intake), intravascular administration, enteral administration, transdermal administration, and intraperitoneal administration. Moreover, the dosage form in the case of using as said pharmaceutical is not specifically limited. Specific examples of the dosage form include powders, powders, granules, tablets, capsules, pills, suppositories, liquids, and injections. For example, excipients, bases, emulsifiers, solvents, stabilizers and the like can be contained in the pharmaceutical.

  The lower limit value of the intake of lemon juice as the composition for activating osteoblasts comprising the present composition and the composition for promoting bone-type alkaline phosphatase is not particularly limited. It is preferable that the amount is equal to or more than 10 ml of lemon juice (corresponding to 450 mg of citric acid) in terms of%. In addition, the upper limit of the intake amount of the composition for activating osteoblasts and the composition for promoting bone-type alkaline phosphatase comprising the present composition is not particularly limited, but for example, converted to 100% per adult day It is preferable that the amount is less than or equal to 200 ml of lemon juice (equivalent to 9000 mg of citric acid). Even if ingested in an amount exceeding 200 ml, the effect obtained is equivalent to that ingesting an amount equivalent to 200 ml due to the saturation of the osteoblast activation action and the bone-type alkaline phosphatase promoting action.

  The amount of lemon juice converted to 100% is the amount converted to straight fruit juice (or concentrated and reduced 100% lemon juice). In addition, since 1 ml of straight fruit juice contains about 45 mg of citric acid, the lower limit value and the upper limit value of the intake amount can be converted by the citric acid amount.

  By regulating the intake of the composition for activating osteoblasts and the composition for promoting bone-type alkaline phosphatase comprising this composition within the above ranges, the osteoblast activation action and the bone-type alkaline phosphatase promotion action can be further improved. Can be improved. Moreover, it can be ingested efficiently.

Next, effects of the above embodiment will be described.
(1) The composition for activating osteoblasts comprises lemon juice as an active ingredient.
According to the said structure, the effect which activates an osteoblast is acquired. Activation of osteoblasts can be confirmed by an increase in bone formation markers (for example, bone-type alkaline phosphatase and osteocalcin) that are indicators of bone formation by osteoblasts.

(2) Further, as a result of activation of osteoblasts and promotion of bone formation, an effect of improving bone density is also obtained.
(3) Moreover, since it is not necessary to ingest calcium in combination, the effect of activating osteoblasts and the effect of improving bone density are expected without greatly changing the diet.

(4) Moreover, since it is not necessary to use a high-load exercise together, an effect of activating osteoblasts and an improvement effect of bone density are expected even for a person with limited exercise.
(5) The composition for improving bone density of the present embodiment can preferably activate osteoblasts and improve bone density, particularly in elderly people (for example, postmenopausal women).

(6) The composition for promoting bone-type alkaline phosphatase contains lemon juice as an active ingredient.
According to the above configuration, an effect of increasing bone alkaline phosphatase can be obtained. Moreover, the same effect as said (2)-(5) is also acquired by this.

In addition, this embodiment can also be changed and embodied as follows.
-The composition for activating osteoblasts and the composition for promoting bone-type alkaline phosphatase of the above embodiment may each contain components other than lemon juice as long as the intended action is not impaired.

  The intake amount and intake period of the osteoblast activation composition and the bone-type alkaline phosphatase promoting composition of the above embodiment are not particularly limited, and the state of the physical function, age, sex, and others of the intake person It is determined appropriately in consideration of the above conditions. For example, in order to promote the activation action of osteoblasts or promote bone-type alkaline phosphatase, it is preferable to continuously take a certain amount that exhibits the effect for several months or more.

  -The composition for activating osteoblasts and the composition for promoting bone-type alkaline phosphatase of the above embodiment can be applied not only to foods and drinks or medicines consumed by humans, but also to raising livestock etc. You may apply to the feed, medicine, etc. with respect to an animal.

The above embodiment will be described more specifically with reference to test examples. The present invention is not limited to these.
<Preparation of test beverage>
100% equivalent lemon juice 30.5ml (1372.5mg of citric acid) reduced from concentrated juice, 2.9g of fructose-glucose solution, 0.03g of sweetener, and 0.8g of fragrance are mixed, resulting in a total of 290ml. A test beverage was prepared by adding water as described.

<Evaluation test>
Changes in bone density, bone resorption marker, and bone formation marker when the test beverage was continuously ingested were evaluated.

  Sixty post-menopausal middle-aged and older women were randomly divided into a first group (27) and a second group (33). The subjects of the first group were allowed to ingest one test drink (290 ml) per day continuously for 5 months. Subjects in Group 2 were not allowed to ingest the test beverage for 5 months. Each subject was instructed not to change their lifestyle habits, such as doing special exercise or intentionally ingesting calcium.

  BAP (bone-type alkaline phosphatase) in serum as an osteogenesis marker and Tracp-5b (bone-type tartrate-resistant acid phosphatase 5b) in serum as a bone resorption marker for each of 2 months and 5 months before ingestion, Bone density was measured and the average value was calculated. These results are shown in Tables 1-3.

  BAP was measured by a chemiluminescent enzyme immunoassay (CLEIA method) using a commercially available test agent. Tracp-5b was measured by enzyme immunoassay (EIA method) using a commercially available test drug. The bone density was determined by measuring the lumbar bone density with an X-ray bone density measuring device.

As shown in Table 1, it was confirmed that when the test beverage was continuously ingested, the bone formation marker was significantly increased. On the other hand, as shown in Table 2, a change in the bone resorption marker due to continuous intake of the test beverage could not be confirmed. From these results, the test beverage as an osteogenesis promoting composition activates osteoblasts, but has a great effect on osteoclasts that act on bone resorption (degradation of old bone). Guess that there is not.

  In addition, as shown in Table 3, when the test beverage is not continuously ingested, the bone density decreases, whereas when the test beverage is continuously ingested, the bone density is maintained. Or the tendency to rise was confirmed. Regarding this result, it is presumed that bone density was improved by activation of osteoblasts and promotion of bone formation.

Next, the technical idea that can be grasped from the embodiment and the modified examples will be described.
(I) A composition for promoting bone formation, comprising lemon juice as an active ingredient.
(B) The osteoblast activation composition, the bone formation promoting composition, or the bone-type alkaline phosphatase promoting composition intended for the elderly.

(C) A composition for improving bone density which does not require the combined use of calcium, characterized by using lemon juice as an active ingredient.

Claims (3)

  An osteoblast activation composition comprising lemon juice as an active ingredient.   2. The composition for activating osteoblasts according to claim 1, comprising the lemon juice in an amount equivalent to 10 ml of lemon juice in 100% conversion.   A composition for promoting bone-type alkaline phosphatase, comprising lemon juice as an active ingredient.