JP2017067667A - Saliva sampling chewing gum - Google Patents
Saliva sampling chewing gum Download PDFInfo
- Publication number
- JP2017067667A JP2017067667A JP2015195426A JP2015195426A JP2017067667A JP 2017067667 A JP2017067667 A JP 2017067667A JP 2015195426 A JP2015195426 A JP 2015195426A JP 2015195426 A JP2015195426 A JP 2015195426A JP 2017067667 A JP2017067667 A JP 2017067667A
- Authority
- JP
- Japan
- Prior art keywords
- saliva
- mass
- gum
- melting point
- glycerol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003296 saliva Anatomy 0.000 title claims abstract description 51
- 229940112822 chewing gum Drugs 0.000 title abstract description 5
- 235000015218 chewing gum Nutrition 0.000 title abstract description 5
- 238000005070 sampling Methods 0.000 title abstract 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 35
- 238000002844 melting Methods 0.000 claims abstract description 18
- 230000008018 melting Effects 0.000 claims abstract description 18
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 14
- 239000000194 fatty acid Substances 0.000 claims abstract description 14
- 229930195729 fatty acid Natural products 0.000 claims abstract description 14
- -1 glycerol fatty acid ester Chemical class 0.000 claims abstract description 14
- 229920002367 Polyisobutene Polymers 0.000 claims abstract description 8
- 239000004200 microcrystalline wax Substances 0.000 claims abstract description 8
- 235000019808 microcrystalline wax Nutrition 0.000 claims abstract description 8
- 235000010985 glycerol esters of wood rosin Nutrition 0.000 claims abstract description 5
- 235000011187 glycerol Nutrition 0.000 claims description 20
- GHBFNMLVSPCDGN-UHFFFAOYSA-N rac-1-monooctanoylglycerol Chemical compound CCCCCCCC(=O)OCC(O)CO GHBFNMLVSPCDGN-UHFFFAOYSA-N 0.000 claims description 4
- ARIWANIATODDMH-AWEZNQCLSA-N 1-lauroyl-sn-glycerol Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)CO ARIWANIATODDMH-AWEZNQCLSA-N 0.000 claims description 2
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 2
- RZRNAYUHWVFMIP-KTKRTIGZSA-N 1-oleoylglycerol Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(O)CO RZRNAYUHWVFMIP-KTKRTIGZSA-N 0.000 claims description 2
- ULQISTXYYBZJSJ-UHFFFAOYSA-N 12-hydroxyoctadecanoic acid Chemical compound CCCCCCC(O)CCCCCCCCCCC(O)=O ULQISTXYYBZJSJ-UHFFFAOYSA-N 0.000 claims description 2
- LKUNXBRZDFMZOK-GFCCVEGCSA-N Capric acid monoglyceride Natural products CCCCCCCCCC(=O)OC[C@H](O)CO LKUNXBRZDFMZOK-GFCCVEGCSA-N 0.000 claims description 2
- ARIWANIATODDMH-UHFFFAOYSA-N Lauric acid monoglyceride Natural products CCCCCCCCCCCC(=O)OCC(O)CO ARIWANIATODDMH-UHFFFAOYSA-N 0.000 claims description 2
- BYNVYIUJKRRNNC-UHFFFAOYSA-N docosanoic acid;propane-1,2,3-triol Chemical compound OCC(O)CO.CCCCCCCCCCCCCCCCCCCCCC(O)=O BYNVYIUJKRRNNC-UHFFFAOYSA-N 0.000 claims description 2
- RZRNAYUHWVFMIP-HXUWFJFHSA-N glycerol monolinoleate Natural products CCCCCCCCC=CCCCCCCCC(=O)OC[C@H](O)CO RZRNAYUHWVFMIP-HXUWFJFHSA-N 0.000 claims description 2
- LKUNXBRZDFMZOK-UHFFFAOYSA-N rac-1-monodecanoylglycerol Chemical compound CCCCCCCCCC(=O)OCC(O)CO LKUNXBRZDFMZOK-UHFFFAOYSA-N 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 12
- 239000012528 membrane Substances 0.000 abstract description 6
- 238000010340 saliva test Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 238000003317 immunochromatography Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000000654 additive Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Confectionery (AREA)
- Medicinal Preparation (AREA)
Abstract
Description
本発明は唾液の検査に最適なガムに関する。 The present invention relates to a gum that is optimal for examination of saliva.
歯科医療においては、唾液の検査を行い口腔内細菌の検出やう蝕リスクの程度を調べている。（例えば、特許文献１参照。）。被験者から唾液を採取する方法としては、例えば唾液吸引装置を用いる方法等が用いられている（例えば、特許文献２参照。）。 In dentistry, saliva is examined to detect oral bacteria and the degree of caries risk. (For example, refer to Patent Document 1). As a method for collecting saliva from a subject, for example, a method using a saliva suction device or the like is used (for example, see Patent Document 2).
しかしこの方法は、唾液吸引装置自体が大がかりであり導入コストが高いという問題があるため、被験者に数分間ガムを噛ませ分泌された唾液をロート等の採取容器に吐き出して採取する方法が行われている。 However, since this method has a problem that the saliva suction device itself is large and the introduction cost is high, there is a method in which the subject is chewed with gum for several minutes and the secreted saliva is discharged into a collection container such as a funnel and collected. ing.
この方法によれば唾液の分泌を促進させ比較的容易に唾液を採取することができる。しかし、従来のガムでは、例えばイムノクロマト法を用いた試験を行う際に、唾液の粘性が高いためにイムノクロマト試験片である多孔質膜を唾液がうまく通過せずに試験ができない場合があった。そのため、イムノクロマト試験の様な多孔質膜を通過させる唾液検査に適した粘性の低い唾液を採取することができる新たな唾液採取用ガムが求められていた。 According to this method, saliva can be collected relatively easily by promoting the secretion of saliva. However, in the case of a conventional gum, for example, when a test using an immunochromatography method is performed, the viscosity of saliva is high, so that the test may not be performed because saliva does not pass through the porous membrane as an immunochromatographic test piece. Therefore, there has been a demand for a new saliva-collecting gum that can collect saliva with low viscosity suitable for saliva testing that passes through a porous membrane such as an immunochromatographic test.
イムノクロマト試験の様な多孔質膜を通過させる唾液検査に適した粘性の低い唾液を採取することができる、唾液採取用のガムを提供することを目的とする。 An object of the present invention is to provide a saliva-collecting gum capable of collecting saliva having a low viscosity suitable for a saliva test that passes through a porous membrane such as an immunochromatographic test.
本発明は、ポリイソブチレン及びマイクロクリスタリンワックス：８０〜９５質量％，融点が６５℃以上のグリセリン脂肪酸エステル：１〜１０質量％，融点が６０℃未満のグリセリン脂肪酸エステル：１〜１０質量％，エステルガム：１〜１０質量％を含むことを特徴とする唾液採取用ガムとすると、前記課題を解決することが可能であることを見出して本発明を完成させた。 The present invention relates to polyisobutylene and microcrystalline wax: 80 to 95% by mass, glycerin fatty acid ester having a melting point of 65 ° C. or higher: 1 to 10% by mass, glycerin fatty acid ester having a melting point of less than 60 ° C .: 1 to 10% by mass, ester Gum: The present invention has been completed by finding that the above-mentioned problems can be solved when the gum for saliva collection is characterized by containing 1 to 10% by mass.
本発明に係る唾液採取用ガムは、イムノクロマト試験の様な多孔質膜を通過させる唾液検査に適した粘性の低い唾液を採取することができる唾液採取用ガムである。 The saliva-collecting gum according to the present invention is a saliva-collecting gum that can collect saliva with low viscosity suitable for a saliva test that passes through a porous membrane such as an immunochromatographic test.
以下、本発明に係る唾液採取用ガムについて詳細に説明する。 Hereinafter, the gum for saliva collection according to the present invention will be described in detail.
本発明の唾液採取用ガムは、ポリイソブチレン及びマイクロクリスタリンワックスをガムベースとする。ポリイソブチレン及びマイクロクリスタリンワックスは公知の物質を使用することができる。 The gum for saliva collection according to the present invention is based on polyisobutylene and microcrystalline wax. Known materials can be used as the polyisobutylene and the microcrystalline wax.
ポリイソブチレン及びマイクロクリスタリンワックスの配合量は唾液採取用ガム全体の８０〜９５質量％である。
また、ポリイソブチレン及びマイクロクリスタリンワックスは混合して使用され、質量比は２：１〜１：２であることが好ましい。
The blending amount of the polyisobutylene and the microcrystalline wax is 80 to 95% by mass of the whole saliva collection gum.
In addition, polyisobutylene and microcrystalline wax are used as a mixture, and the mass ratio is preferably 2: 1 to 1: 2.
本発明の唾液採取用ガムは、特定のグリセリン脂肪酸エステルを含む。具体的には、融点が６５℃以上のグリセリン脂肪酸エステル：１〜１０質量％，及び融点が６０℃未満のグリセリン脂肪酸エステル：１〜１０質量％である。これら特定のグリセリン脂肪酸エステルを配合することによって、粘性の低い刺激唾液を採取することができる。 The saliva collection gum of the present invention contains a specific glycerin fatty acid ester. Specifically, the glycerin fatty acid ester having a melting point of 65 ° C. or higher: 1 to 10% by mass, and the glycerin fatty acid ester having a melting point of less than 60 ° C .: 1 to 10% by mass. By blending these specific glycerin fatty acid esters, stimulated saliva with low viscosity can be collected.
融点が６５℃以上のグリセリン脂肪酸エステルは、グリセリンモノステアレート（融点６５〜７０℃）），グリセリンモノベヘネート（７５〜８０℃），グリセリンモノ１２−ヒドロキシステアレート（融点７０〜７８℃），グリセリンモノジベヘネート（６９〜７５℃）を例示することができる。これらの配合量は唾液採取用ガム全体の１〜１０質量％であることが必要であり、１質量％未満または１０質量％を超えて配合すると粘性の低い刺激唾液を採取することができない。 Glycerin fatty acid ester having a melting point of 65 ° C. or higher is glycerin monostearate (melting point 65 to 70 ° C.)), glycerin monobehenate (75 to 80 ° C.), glycerin mono 12-hydroxystearate (melting point 70 to 78 ° C.), Examples thereof include glycerin monodibehenate (69 to 75 ° C.). These blending amounts must be 1 to 10% by mass of the saliva collection gum as a whole, and if the blending amount is less than 1% by mass or more than 10% by mass, the stimulated saliva with low viscosity cannot be collected.
融点が６０℃未満のグリセリン脂肪酸エステルは、グリセリンモノオレート（３３〜３７℃），グリセリンモノカプリレート（融点３０〜３３℃），グリセリンモノカプレート（融点４５〜４７℃），グリセリンモノラウレート（融点５５〜５７℃），グリセリンモノジステアレート（融点５０〜６０），グリセリンモノジオレート（２２〜２５℃）を例示することができる。これらの配合量は唾液採取用ガム全体の１〜１０質量％であることが必要であり、１質量％未満または１０質量％を超えて配合すると粘性の低い刺激唾液を採取することができない。 Glycerin fatty acid esters having a melting point of less than 60 ° C. are glycerol monooleate (33 to 37 ° C.), glycerol monocaprylate (melting point 30 to 33 ° C.), glycerol monocaprate (melting point 45 to 47 ° C.), glycerol monolaurate (melting point) 55-57 ° C.), glycerin monodistearate (melting point: 50-60), and glycerin monodiolate (22-25 ° C.). These blending amounts must be 1 to 10% by mass of the saliva collection gum as a whole, and if the blending amount is less than 1% by mass or more than 10% by mass, the stimulated saliva with low viscosity cannot be collected.
本発明の唾液採取用ガムは、エステルガムを含む。エステルガムは、唾液採取用ガム全体の１〜５質量％の範囲で配合される。 The saliva collection gum of the present invention includes an ester gum. Ester gum is mix | blended in the range of 1-5 mass% of the whole gum for saliva collection.
本発明の唾液採取用ガムには、安定剤，保存料は適宜配合されてもよいが、香料や甘味料等の唾液の分泌に影響を与える添加剤は配合されないことが好ましい。 In the saliva collection gum of the present invention, stabilizers and preservatives may be appropriately blended, but it is preferable not to blend additives that affect saliva secretion such as fragrances and sweeteners.
以下、実施例及び比較例を示して、本発明をさらに詳細に説明するが、本発明はこれらの実施例に限定されるものではない。 EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated further in detail, this invention is not limited to these Examples.
「イムノクロマト法を用いた唾液中口腔内細菌の検査」
被験者にガムを噛ませることで唾液を採取した後、該唾液を処理（アルカリ液で粘性を低下させた後、酸性の液体を加えて中和）し、これをイムノクロマト法を用いた唾液検査製品（製品名 サリバチェックミュータンス：ジーシー社製）に用いた。
“Inspection of oral bacteria in saliva using immunochromatography”
After collecting saliva by chewing gum on the test subject, the saliva is treated (decrease the viscosity with an alkaline solution and then neutralized by adding an acidic liquid), and this is a saliva test product using immunochromatography (Product name: Saliva check mutans: manufactured by GC Corporation).
＜評価方法＞
使用した唾液検査製品の規定時間内でのコントロールライン及びテストラインの有無を確認する。コントロールラインは、多孔質内を唾液が問題なく流れたことを示すもので、コントロールラインが出なければ、唾液の粘性が高く正常に流れていないことを示している。
<Evaluation method>
Check the presence or absence of the control line and test line within the specified time of the saliva test product used. The control line indicates that saliva has flowed through the porous body without any problem. If the control line does not come out, it indicates that the saliva has a high viscosity and does not flow normally.
＜実施例１〜５＞
従来のガムによる採取では唾液の粘性が高く、コントロールラインが現れなかった被験者のみを５名選出した。当該被験者に表１に示した本発明の唾液採取用ガムを使用し、コントロールラインが出るか否かを確認した。コントロールラインが表れた人数が４名以上であった場合を○、３名以下であった場合を×として評価した。結果を表１に纏めて示す。
<Examples 1-5>
In the conventional collection with gum, only five subjects were selected who had high saliva viscosity and no control line. Using the saliva collection gum of the present invention shown in Table 1 for the subject, it was confirmed whether or not the control line appeared. The case where the number of persons who appeared on the control line was 4 or more was evaluated as ○, and the case where it was 3 or less was evaluated as ×. The results are summarized in Table 1.
「唾液緩衝能測定用ストリップによる検査」
被験者にガムを噛ませることで唾液を採取した後、該唾液を処理（アルカリ液で粘性を低下させた後、酸性の液体を加えて中和）し、多孔質膜を用いた唾液緩衝能測定用ストリップ（製品名 サリバチェックバッファ：ジーシー社製）に用いた。
"Inspection with strips for measuring saliva buffer capacity"
After collecting saliva by chewing gum on the test subject, the saliva is treated (decrease the viscosity with an alkaline solution, then neutralized by adding an acidic liquid), and the saliva buffer capacity measurement using a porous membrane Strip (product name: Saliva check buffer: manufactured by GC Corporation).
＜評価方法＞
唾液緩衝能測定用ストリップ中を唾液が流れて試験が可能であるか否かを確認した。検査が可能であった人数が４名以上であった場合を○、３名以下であった場合を×として評価した。結果を表１に纏めて示す。
<Evaluation method>
It was confirmed whether or not the test was possible with saliva flowing through the strip for measuring saliva buffering capacity. The case where the number of persons who could be inspected was 4 or more was evaluated as ○, and the case where it was 3 or less was evaluated as ×. The results are summarized in Table 1.
Claims (3)
融点が６５℃以上のグリセリン脂肪酸エステル：１〜１０質量％
融点が６０℃未満のグリセリン脂肪酸エステル：１〜１０質量％
エステルガム：１〜５質量％
を含むことを特徴とする唾液採取用ガム。 Polyisobutylene and microcrystalline wax: 80 to 95% by mass
Glycerin fatty acid ester having a melting point of 65 ° C. or higher: 1 to 10% by mass
Glycerin fatty acid ester having a melting point of less than 60 ° C .: 1 to 10% by mass
Ester gum: 1-5% by mass
A saliva collecting gum characterized by containing.
融点が６０℃未満のグリセリン脂肪酸エステルがグリセリンモノオレート，グリセリンモノカプリレート，グリセリンモノカプレート，グリセリンモノラウレート，グリセリンモノジステアレート，グリセリンモノジオレートから選らばれる一種または二種以上
である請求項１または２に記載の唾液採取用ガム。 The glycerin fatty acid ester having a melting point of 65 ° C. or higher is one or more selected from glycerin monostearate, glycerin monobehenate, glycerin mono12-hydroxystearate, glycerin monodibehenate,
The glycerol fatty acid ester having a melting point of less than 60 ° C is one or more selected from glycerol monooleate, glycerol monocaprylate, glycerol monocaprate, glycerol monolaurate, glycerol monodistearate, and glycerol monodiolate. The saliva collecting gum according to 1 or 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015195426A JP6496649B2 (en) | 2015-09-30 | 2015-09-30 | Saliva collection gum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015195426A JP6496649B2 (en) | 2015-09-30 | 2015-09-30 | Saliva collection gum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017067667A true JP2017067667A (en) | 2017-04-06 |
| JP6496649B2 JP6496649B2 (en) | 2019-04-03 |
Family
ID=58494495
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015195426A Active JP6496649B2 (en) | 2015-09-30 | 2015-09-30 | Saliva collection gum |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6496649B2 (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4317837A (en) * | 1980-11-25 | 1982-03-02 | Life Savers, Inc. | Tobacco-flavored chewing gum |
| JPH0383920A (en) * | 1989-08-14 | 1991-04-09 | Warner Lambert Co | Method of simulating saliva secretion of xerostomia patient |
| US6015681A (en) * | 1995-07-28 | 2000-01-18 | The United States Of America As Represented By The Secretary Of The Navy | Rapid immunoassay for cariogenic bacteria |
| JP2001500742A (en) * | 1996-09-25 | 2001-01-23 | シューズッカー アクチエンゲゼルシャフト マンハイム／オクセンフルト | Chewing gum with sweetener |
| US20060108279A1 (en) * | 2003-06-02 | 2006-05-25 | Kloos Steven D | Materials and methods for processing non-aqueous mixtures |
| JP2009052945A (en) * | 2007-08-24 | 2009-03-12 | Sunstar Inc | Treatment method of specimen originated in oral cavity inside in immunochromatographic examination |
| JP2012070675A (en) * | 2010-09-29 | 2012-04-12 | Kazuo Hayashi | Powder gum for tableting and tableting chewing gum |
| JP2016031306A (en) * | 2014-07-29 | 2016-03-07 | 株式会社松風 | Inspection method of periodontal disease and inspection diagnostic kit |
-
2015
- 2015-09-30 JP JP2015195426A patent/JP6496649B2/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4317837A (en) * | 1980-11-25 | 1982-03-02 | Life Savers, Inc. | Tobacco-flavored chewing gum |
| JPH0383920A (en) * | 1989-08-14 | 1991-04-09 | Warner Lambert Co | Method of simulating saliva secretion of xerostomia patient |
| US6015681A (en) * | 1995-07-28 | 2000-01-18 | The United States Of America As Represented By The Secretary Of The Navy | Rapid immunoassay for cariogenic bacteria |
| JP2001500742A (en) * | 1996-09-25 | 2001-01-23 | シューズッカー アクチエンゲゼルシャフト マンハイム／オクセンフルト | Chewing gum with sweetener |
| US20060108279A1 (en) * | 2003-06-02 | 2006-05-25 | Kloos Steven D | Materials and methods for processing non-aqueous mixtures |
| JP2009052945A (en) * | 2007-08-24 | 2009-03-12 | Sunstar Inc | Treatment method of specimen originated in oral cavity inside in immunochromatographic examination |
| JP2012070675A (en) * | 2010-09-29 | 2012-04-12 | Kazuo Hayashi | Powder gum for tableting and tableting chewing gum |
| JP2016031306A (en) * | 2014-07-29 | 2016-03-07 | 株式会社松風 | Inspection method of periodontal disease and inspection diagnostic kit |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6496649B2 (en) | 2019-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Blainey et al. | Allergic respiratory disease in grain workers: the role of storage mites | |
| Srinivasan et al. | Comparative evaluation of formocresol and mineral trioxide aggregate as pulpotomy agents in deciduous teeth | |
| Agholme et al. | Changes of periodontal status in patients with Down syndrome during a 7-year period. | |
| Liu et al. | Clinical and microbiologic effect of nonsurgical periodontal therapy on patients with chronic or aggressive periodontitis. | |
| Zander et al. | Risk factors for dental caries in small rural and regional Australian communities | |
| Branco-de-Almeida et al. | Salivary IgA and periodontal treatment needs in diabetic patients | |
| Feng et al. | Distribution of 8 periodontal microorganisms in family members of Chinese patients with aggressive periodontitis | |
| Glock et al. | Chemical investigation of salivary calculus | |
| Bilaiya et al. | Comparative evaluation of accuracy of iPex, Root Zx mini, and Epex Pro apex locators in teeth with artificially created root perforations in presence of various intracanal irrigants | |
| BR112014017226B1 (en) | METHOD TO STABILIZE FATTY ACIDS AND METHOD OF PREPARATION OF A SOLID MEDIUM | |
| Zhao et al. | Evaluation of periodontitis and bone loss in patients undergoing hemodialysis | |
| Adl et al. | Comparison of push-out bond strength of mineral trioxide aggregate and calcium enriched mixture cement as root end filling materials | |
| Mustafa et al. | Diffusion of calcium hydroxide through dentinal tubules of retreated root canals: An in vitro study | |
| JP6496649B2 (en) | Saliva collection gum | |
| Cui et al. | Oral mycobiome differences in various spatial niches with and without severe early childhood caries | |
| Tar et al. | Oral and dental conditions of children with selective IgA deficiency | |
| Cisneros‐Cabello et al. | Relationship of patient complaints and signs to histopathologic diagnosis of pulpal condition | |
| NZ764338A (en) | Method of assessing growth hormone deficiency in humans by a macimorelin containing composition | |
| Forte et al. | Urinary fluoride excretion in children exposed to fluoride toothpaste and to different water fluoride levels in a tropical area of Brazil | |
| JP6063940B2 (en) | Hardener paste and alginate curable composition preparation kit | |
| WO2012109258A3 (en) | Compositions adn methods for monitoring oxalate | |
| Triches et al. | Microbial reduction by two chemical-mechanical protocols in primary teeth with pulp necrosis and periradicular lesion-an in vivo study | |
| Yu et al. | Effects of saliva contamination on bonding performance of self-etching adhesives | |
| Ancuta et al. | Specific sialochemical and sialometric changes and cariogenic risk in patients with primary Sjogren „s syndrome | |
| MaTsuda et al. | A comparison of clinical, histological and radiographic findings in periapical radiolucid lesions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20180406 |
|
| TRDD | Decision of grant or rejection written | ||
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20190227 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20190305 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20190311 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6496649 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |