JP2017035079A - Microtube, cell housing recovery tool set, and cell recovery auxiliary tool - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a microtube that can preferably recover even a small amount of cells in the case of using a cell collection tool whose tip is formed in a needle-shape.SOLUTION: A microtube 1 comprises: a cylindrical container body 10 in which an opening 11 is formed on one end side and a closed bottom part 12 is formed on the other end side; and a lid 30 which is attached to a container body 10 and seals the opening 11, in which an inner surface of the bottom part 12 of the container body 10 is formed in a concave curved surface with the radius of 0.8 mm or less.SELECTED DRAWING: Figure 5

Description

  The present invention relates to a microtube for storing cells and the like, a cell storage / recovery instrument set, and a cell recovery aid. More specifically, the present invention relates to a microtube, a cell storage / collection instrument set, and a cell collection aid that can suitably collect cells when a cell collection instrument having a needle-like tip is used.

  Conventionally, in regenerative medicine, research has been conducted to promote tissue regeneration by transplanting human or animal cells cultured outside the body, and some have been put into practical use. Such cells are stored and transported in a state of being accommodated in a microtube capable of accommodating a small amount (for example, 500 μL to 1500 μL) of liquid (for example, see Patent Document 1).

Japanese Patent Laid-Open No. 2003-130764

Here, when transplanting cultured cells, the cells collected at the bottom of the microtube by centrifugation are collected using a cell collection device with a needle-like tip such as an injection needle, Cells are injected into a predetermined site. As described above, when a needle with a thin tip is used, it is difficult to collect the cells collected in the lower part without leaving them in the conventional microtube.
In particular, in the field of neurosurgery and the like, cell recovery using a long needle is used to transplant cells into the deep part of the brain, but a small amount of cells contained in a microtube are recovered without using a long needle. It was extremely difficult.

  Accordingly, the present invention provides a microtube, a cell storage / recovery instrument set, and a cell recovery aid that can suitably recover even a small amount of cells when a cell sampling instrument having a needle-like tip is used. The purpose is to do.

  The present invention includes a cylindrical container body having an opening formed at one end and a bottom closed at the other end, and a lid attached to the container body and sealing the opening. It is a microtube provided, Comprising: The inner surface of the said bottom part of the said container main body is related with the microtube formed in a concave curved surface with a radius of 0.8 mm or less.

  The inner surface of the container body includes a tapered surface portion that is continuous with the inner surface of the bottom portion and is inclined at a predetermined angle with respect to the axial direction of the container body, and the taper angle of the tapered surface portion with respect to the axial direction is 10 ° or less. It is preferable that

  Moreover, it is preferable that the taper angle with respect to the said axial direction of the said taper surface part is 7.5 degrees or less.

  Moreover, it is preferable that the taper angle with respect to the said axial direction of the said taper surface part is 5 degrees or more.

  Moreover, it is preferable that a microtube is further provided with the leg part extended from the outer surface of the said container main body to the said bottom part side in the axial direction of this container main body, and can be self-supporting.

  Moreover, it is preferable that the inner surface of the container main body further includes a second tapered surface portion that is continuous with the tapered surface portion and is inclined with respect to the axial direction of the container main body by an angle larger than the taper angle of the tapered surface portion.

  Moreover, it is preferable that the capacity | capacitance of the said container main body is 500 microliters-1500 microliters.

  Further, the container body includes a male screw portion formed on the outer peripheral surface on the opening side, and the lid body is configured by a cap having a female screw portion that can be screwed to the male screw portion on the inner peripheral surface. It is preferable.

  The present invention also provides a microtube according to any one of the above, a tube connecting portion connectable to an upper portion of the container main body, and a shaft of the container main body in a state where the tube connecting portion is connected to the container main body. The present invention relates to a cell collection / recovery instrument set including a cell collection auxiliary tool having a cylindrical portion that extends along a direction and communicates with the inside of the container body through the opening.

  The present invention also provides a cylindrical container body having an opening formed at one end and a bottom closed at the other end, and a lid attached to the container body for sealing the opening. A cell collection aid that assists in the collection of cells contained in the container body, the tube connection part being connectable to the upper part of the container body, and the tube connection part being the container The present invention relates to a cell recovery aid comprising: a cylindrical portion that extends along the axial direction of the container main body and communicates with the inside of the container main body through the opening while being connected to the main body.

  ADVANTAGE OF THE INVENTION According to this invention, the microtube which can collect | recover a small amount of cells suitably using the cell collection instrument in which the front end was formed in the shape of a needle, a cell accommodation collection instrument set, and a cell collection auxiliary tool can be provided.

It is a perspective view which shows the microtube which concerns on 1st Embodiment of this invention. It is a disassembled perspective view which shows the microtube of 1st Embodiment. It is a perspective view which shows the longitudinal cross-section of the microtube of 1st Embodiment. It is a figure which shows the AA line cross section of FIG. It is the elements on larger scale of FIG. It is a perspective view which shows one Embodiment of the cell collection assistance tool of this invention. It is a perspective view which shows the state which connected the cell collection auxiliary tool to the container main body of a microtube. It is a longitudinal cross-sectional view of FIG. It is a perspective view which shows the state which is collect | recovering the cell from the container main body which connected the cell collection auxiliary tool. It is sectional drawing which shows the microtube which concerns on 2nd Embodiment of this invention, and is a figure corresponding to FIG. It is the elements on larger scale of FIG. It is a perspective view which shows the modification of a cell collection | recovery assistance tool and a microtube, and is a figure which shows the state which isolate | separated the cell collection | recovery assistance tool and the microtube. It is a perspective view which shows the modification of a cell collection | recovery assistance tool and a microtube, and is a figure which shows the state which connected the cell collection assistance tool to the container main body of the microtube. It is a perspective view which shows other Embodiment 1 of a cell collection | recovery assistance tool. It is a perspective view which shows other Embodiment 2 of a cell collection | recovery assistance tool.

  Hereinafter, preferred embodiments of the microtube of the present invention will be described with reference to the drawings. The microtube of the present invention is used when containing a liquid containing cells used for transplantation in regenerative medicine. More specifically, the microtube has a maximum capacity of 500 μL to 1500 μL.

The microtube 1 of 1st Embodiment is provided with the container main body 10, the leg part 20, and the cap 30 as a cover body as shown in FIG.1 and FIG.2.
The container body 10 is configured in a cylindrical shape in which an opening 11 is formed on one end side (upper end side) and a bottom 12 closed on the other end side is formed. As shown in FIGS. 3 and 4, the inner surface 13 of the container body 10 includes a concave surface portion 131, a tapered surface portion 132, and a cylindrical surface portion 133.

The concave portion 131 constitutes the inner surface of the bottom portion 12 of the container body 10. As shown in FIG. 5, the concave portion 131 is formed in a concave curved surface having a radius R of 0.8 mm or less. In the present invention, the cell collection device in which the tip of the cell such as an injection needle is formed into a needle shape by forming the concave surface portion 131 into a concave curved surface having a radius R of 0.8 mm or less. Can be effectively recovered (collected).
The radius R of the concave surface portion 131 is preferably 0.5 mm or less, and more preferably 0.25 mm or less, from the viewpoint of increasing the cell recovery rate by the cell collection device having the tip formed in a needle shape. In addition, the radius R of the concave surface portion 131 is preferably 0.1 mm or more from the viewpoint of preventing the collection of cells by the tip of the cell collection device being in close contact with the concave surface portion 131.
In the first embodiment, the radius R of the concave surface portion 131 is set to 0.25 mm.

The tapered surface portion 132 is continuous with the concave surface portion 131 (the inner surface of the bottom portion 12) and is inclined at a predetermined angle with respect to the axial direction LD of the container body 10. In other words, the tapered surface portion 132 is formed in the shape of a conical surface disposed on the upper portion of the concave surface portion 131. The taper angle θ of the taper surface portion 132 with respect to the axial direction LD suitably secures the height (thickness) of the cell layer when gathered on the bottom 12 side of the microtube 1 and the tip has a needle-like shape. From the viewpoint of facilitating the collection of cells by the collection tool, the angle is preferably 10 ° or less, and more preferably 7.5 ° or less. In addition, the taper angle θ of the tapered surface portion 132 with respect to the axial direction LD is preferably 5 ° or more from the viewpoint of preventing the collection of the cells from being blocked by the tip of the cell collection device being in close contact with the tapered surface portion 132.
In the first embodiment, the taper angle θ of the taper surface portion 132 with respect to the axial direction LD is set to 7 °.

  The cylindrical surface portion 133 is disposed on one end side of the container body 10. The cylindrical surface portion 133 is formed on the same circumferential surface as the inner diameter of the container main body 10 at the upper end portion (the end portion on the opening 11 side) of the tapered surface portion 132.

A male screw portion 14 and a container rib portion 15 are formed on the outer surface of the container body 10.
The male screw portion 14 is formed on the outer surface of the container body 10 on the opening 11 side. The container rib portion 15 is disposed closer to the bottom portion 12 than the male screw portion 14 in the container main body 10. The container rib portion 15 is formed such that a part of the outer surface of the container body 10 protrudes over the entire circumference of the container body 10.

  The leg portion 20 extends from the outer surface of the container body 10 to the bottom 12 side in the axial direction LD of the container body 10. In the first embodiment, the leg portion 20 is formed to extend in a cylindrical shape from the outer peripheral surface of the container body 10 toward the bottom portion 12 side. As shown in FIG. 4, the position of the distal end portion of the leg portion 20 is located on the distal end side of the bottom portion 12 of the container body 10. Further, the distal end surface of the leg portion 20 is formed in a planar shape orthogonal to the axial direction LD of the microtube 1. Thereby, the microtube 1 is comprised so that it can stand by the leg part 20 in the state which orient | assigned the bottom part 12 below.

In the first embodiment, the container body 10 and the legs 20 are formed by integrally molding a synthetic resin member. The container body 10 and the legs 20 are preferably made of a material having transparency from the viewpoint of enabling the contained cells to be visually recognized, and are preferably made of a material having relatively high rigidity. Specifically, the material constituting the container body 10 is polycarbonate, polyethylene, polypropylene, polyester, polymethylpentene, methacryl, ABS resin (acrylonitrile / butadiene / styrene copolymer), PET resin (polyethylene terephthalate), poly Examples thereof include synthetic resins such as vinyl chloride.
Moreover, the diameter (inner diameter) in the opening part 11 of the container main body 10 is preferably 6 mm to 10 mm, and the height of the container main body 10 is preferably 30 mm to 70 mm.

The cap 30 is attached to the container body 10 and seals the opening 11. In the present embodiment, the cap 30 is configured separately from the container body 10. The cap 30 is formed in a cylindrical shape whose one end side is closed on which an internal thread portion 31 that can be screwed into the external thread portion 14 of the container body 10 is formed on the inner peripheral surface.
The cap 30 can be made of the same material as the container body 10.

  The microtube 1 of the first embodiment described above is suitably used when transplanting cultured cells into a living body in regenerative medicine. Specifically, when transplanting cultured cells into a living body, the cells used for transplantation are housed in the microtube 1 together with other liquid components (such as a culture solution), and the cap 30 is attached to the container body 10. In a centrifuge. As a result, the cells are collected at the bottom 12 (near the concave portion 131) of the microtube 1 (container body 10) and separated from the liquid component.

  Next, the cap 30 is removed from the container body 10 and the cells collected on the bottom 12 are collected using a cell collection device. When transplanting cells, in order to inject the collected cells into a predetermined site in the living body, a cell collection instrument having a needle-like tip such as an injection needle is used.

Here, a conventional microtube having a flat bottom inner surface and a microtube having a small curvature such that the radius R of the concave curved surface exceeds 0.8 mm although the inner surface of the bottom is formed by a concave curved surface. In this case, when a cell collection device such as a micropipette whose tip is not formed in a needle shape is used, the cells can be suitably recovered. However, when a cell collection device having a needle-like tip is used, it is difficult to collect all the cells with such a conventional microtube.
Therefore, in the first embodiment, the concave surface portion 131 that is the inner surface of the bottom portion 12 of the container body 10 is formed into a concave curved surface having a radius of 0.8 mm or less. Thereby, the cells accommodated in the microtube 1 can be effectively recovered by using a cell collection instrument having a needle-like tip such as an injection needle.

  Further, the inner surface 13 of the container main body 10 is configured to include a tapered surface portion 132 that is continuous with the concave surface portion 131 and inclined at a predetermined angle with respect to the axial direction LD of the container main body 10, and the tapered surface portion 132 is tapered relative to the axial direction LD. The angle was configured to be 10 ° or less. Thereby, since the thickness of the cell layer when collected on the bottom 12 side of the microtube 1 can be suitably ensured, the cells can be more easily collected by the cell collecting device having the tip formed in a needle shape.

  Moreover, the microtube 1 was comprised including the leg part 20, and it was made self-supporting. Thereby, the handleability of the microtube 1 can be improved.

  In addition, by applying the configuration of the concave surface portion 131 and / or the tapered surface portion 132 of the first embodiment to the microtube 1 having a capacity of 500 μL to 1500 μL, the cell recovery rate by the cell collection device having the tip formed in a needle shape can be increased. It is preferably increased.

Next, referring to FIGS. 6 to 9, the cell collection assisting tool 50 for assisting the collection of the cells accommodated in the microtube 1 of the present invention and the cell accommodation / collection instrument set 100 including the microtube 1 and the cell collection assisting tool 50 will be described. While explaining.
As shown in FIG. 6, the cell collection aid 50 includes a tube connection part 51 and a cylindrical part 52 that are formed in a cylindrical shape having both ends opened.

  The tube connection part 51 is disposed at one end of the cell collection aid 50 and is configured to be connectable to the upper end of the container body 10 of the microtube 1. In this embodiment, the tube connection part 51 is comprised by the cylinder shape by which the internal thread part 511 which can be screwed together with the external thread part 14 of the container main body 10 was formed in the internal peripheral surface.

  The cylindrical part 52 is formed integrally with the tube connecting part 51. The cylindrical portion 52 extends along the axial direction LD of the container main body 10 in a state where the tube connecting portion 51 is connected to the container main body 10. And in this state, the cylindrical part 52 is connected to the inside of the container main body 10 through the opening part 11 (refer FIG. 8).

The length of the cylindrical portion 52 can be set as appropriate according to the length of the needle of the cell collection instrument used for cell collection. However, the length of the cylindrical portion 52 can be increased by using a long needle used for transplanting cells deep in the brain. From the viewpoint of suitably collecting cells, it is preferably 10 cm to 30 cm. The inner diameter of the cylindrical portion 52 is preferably configured to be the same as the inner diameter of the opening 11 of the container body 10 from the viewpoint of suitably inserting the long needle into the microtube 1.
The cell collection aid 50 described above is made of a hard synthetic resin similar to the material constituting the container body 10.

Next, a method for using the cell collection aid 50 will be described.
The cell collection aid 50 is used when collecting (collecting) the cells collected on the bottom 12 of the container body 10 by centrifugation. When collecting the cells, first, as shown in FIGS. 7 and 8, the female threaded portion 511 of the tube connecting portion 51 is screwed into the male threaded portion 14 of the container body 10 with the cap 30 removed. The tool 50 is connected to the container body 10.
Next, as shown in FIG. 9, the long needle of the cell collection device 200 having the long needle 210 and the syringe 220 is inserted into the cylindrical portion 52. Thereby, the long needle is easily inserted into the opening 11 of the container body 10 by being guided by the cylindrical portion 52.
Next, the tip of the long needle is positioned on the bottom 12 (concave surface 131) of the container body 10 to collect the cells.

  In this way, by using the cell collection aid 50, especially when collecting cells using a cell collection device having a long needle, the long needle can be easily inserted into the microtube 1, so that it is accommodated in the microtube 1. The burden on cell transplantation can be reduced.

Next, the microtube 1A of the second embodiment will be described with reference to FIGS.
The microtube 1A of the second embodiment differs from the first embodiment in the configuration of the tapered surface portion 132A (inner surface 13A). About 2nd Embodiment, a different point from 1st Embodiment mentioned above is mainly demonstrated, the same point attaches | subjects the same code | symbol and abbreviate | omits description. For the points that are not particularly described, the description of the first embodiment is applied as appropriate.

In the microtube 1A of the second embodiment, the tapered surface portion 132A includes a first tapered surface portion 135 and a second tapered surface portion 136. The first tapered surface portion 135 corresponds to the tapered surface portion 132 in the first embodiment.
The second taper surface portion 136 is continuous with the opening 11 side of the first taper surface portion 135 and is inclined at an angle θ2 larger than the taper angle θ of the first taper surface portion 135 with respect to the axial direction LD of the container body 10A.

In 2nd Embodiment, taper angle (theta) with respect to axial direction LD of the 1st taper surface part 135 is set to 5 degrees, and taper angle (theta) 2 with respect to axial direction LD of the 2nd taper surface part 136 is set to 10 degrees ( (See FIGS. 10 and 11).
Moreover, in 2nd Embodiment, the leg part 20 is extended and formed from the outer peripheral surface of the part in which the 2nd taper surface part 136 of the container main body 10 is located.

According to the microtube 1A of the second embodiment, in addition to the same effects as those of the first embodiment, the following effects are also obtained.
The container body 10A includes a first tapered surface portion 135 and a second tapered surface portion 136 that is inclined at an angle θ2 larger than the taper angle θ of the first tapered surface portion 135. Thereby, even if it is a case where taper angle (theta) of the 1st taper surface part 135 is made small, the quantity of the liquid which can be accommodated in the container main body 10 can be increased by adjusting taper angle (theta) 2 of the 2nd taper surface part 136. FIG.

  Moreover, the leg part 20 was extended from the outer peripheral surface of the part in which the 2nd taper surface part 136 of the container main body 10 is located. Thereby, it can prevent that the angle of the extension direction of the leg part 20 and the direction where the 2nd taper surface part 136 is extended becomes small too much. Therefore, when the container main body 10 and the leg part 20 are integrally formed of a synthetic resin member, it is possible to prevent molding defects from occurring at the boundary portion between the tapered surface part 132A and the leg part 20. From the viewpoint of effectively suppressing molding defects at the boundary portion between the tapered surface portion 132A and the leg portion 20, the taper angle θ2 with respect to the axial direction LD of the second tapered surface portion 136 is preferably 10 ° to 30 °.

As mentioned above, although this invention was demonstrated based on the preferable embodiment, this invention is not restrict | limited to the said embodiment, As long as it does not deviate from the meaning of this invention, it can change suitably.
For example, in the first embodiment and the second embodiment, the container main bodies 10 and 10A and the cap 30 are configured separately, but the present invention is not limited thereto. That is, for example, as shown in FIG. 12, the microtube 1B may be configured integrally by connecting the container body 10B and the cap 30B with a band-shaped connecting member 40B. In this case, as shown in FIG. 13, it is preferable that the cell collection assisting tool 50B includes a slit 53B that is formed in the tube connecting portion 51B and into which the connecting member 40B can enter. Thereby, the cell collection | recovery auxiliary tool 50B and the cell accommodation collection | recovery instrument set 100B which can respond also to the microtube 1B which has the connection member 40B are realizable.
In this case, the microtube 1B and the cell collection aid 50B are connected by fitting them both in a liquid-tight manner.

In the above-described embodiment, the end surface (the end surface on the side into which the long needle 210 is inserted) of the tubular portion 52 of the cell collection assisting tool 50 opposite to the tube connecting portion 51 is perpendicular to the axial direction of the tubular portion 52. Although configured with a surface, the present invention is not limited to this. That is, as shown in FIG. 14, the cell collection assisting tool 50C may include a cutout portion 54C in which a part of the end surface on the side where the long hand is inserted is cut out in the axial direction. Further, as shown in FIG. 15, the cell collection assisting tool 50D may be configured by forming the end surface on the side where the long hand is inserted into an inclined surface inclined by a predetermined angle with respect to the axial direction of the cylindrical portion 52D. Thereby, it is possible to easily insert the long needle with the cell collection aid.
When the cell collection aid 50C is configured to include the cutout portion 54C, the width of the cutout portion 54C is not limited to the width shown in FIG. For example, the cutout portion 54C may be formed in a so-called slit shape having a width that allows a long hand to pass therethrough.

  Moreover, in 1st Embodiment and 2nd Embodiment, although the microtubes 1 and 1A were comprised so that it could stand independently including the leg part 20, it is not restricted to this. That is, you may comprise a microtube without including a leg part.

  Moreover, in the said embodiment, although the microtube and the cell collection | recovery assistance tool were connected by screwing or fitting, it is not restricted to this. In other words, other connection methods may be used as long as the cells can be connected in a liquid-tight manner so that cells do not leak from the connection portion between the microtube and the cell collection aid. Moreover, you may comprise a connection part including an O-ring.

  Next, the present invention will be described in more detail based on examples, but the present invention is not limited thereto.

[Measurement of cell recovery rate]
The microtubes of Examples 1 to 3 shown in Table 1 below and the microtubes of the comparative example were prepared, and the ease of cell recovery was measured for each microtube.

The ease of cell recovery was measured by the following method and procedure.
1. 600 mL of bovine blood was centrifuged at 3000 g for 10 minutes.
2. 50 mL of the plasma obtained in “1.” was transferred to a 50 mL centrifuge tube, and the remaining plasma was discarded.
3. The buffy coat (white blood cells and platelets) obtained in “1.” was transferred to a 15 mL centrifuge tube.
4). The buffy coat obtained in “3.” was centrifuged at 2330 g for 10 minutes, and the red blood cell layer was discarded with a dropper.
5). An appropriate amount of the plasma of “2.” was added to “4.” to obtain a cell suspension, and the number of cells (white blood cell count) was counted using a blood cell counter. (As a guide to the white blood cell count, 10 ± 20,000 / uL) * The cell suspension amount was about 8 mL. (Required liquid volume is 6mL or more)
6). 500 uL of cell suspension was dispensed into microtubes (total 4) of Examples 1 to 3 and Comparative Example. (N = 3)
7). “6.” was centrifuged at 200 g for 5 minutes.
Using the 8.23G needle and syringe, the entire cell suspension was recovered and transferred to a sampling tube.
9. The cell count (white blood cell count) was counted using a blood cell counter, and the cell recovery rate was calculated.
10.1-9 was implemented twice. (Total n = 6) The results are shown in Table 2.

  As shown in Table 2, it was confirmed that according to the microtubes of the examples in which the radius R of the concave surface portion was 0.8 mm or less, the cells could be recovered at a high rate of 95% or more. In particular, in the microtube of Example 3 in which the radius R of the concave surface portion is 0.1 mm and the taper angle with respect to the axial direction of the tapered surface portion is 5 °, the cell recovery rate is particularly preferably 99% or more. It was confirmed that it was possible.

1, 1A, 1B Microtube 10, 10A, 10B Container body 11 Opening portion 12 Bottom portion 13 Inner surface 14 Male thread portion 20 Leg portion 30 Cap (lid)
31 Female thread part 50 Cell collection aid 51 Tube connection part 52 Cylindrical part 100, 100B Cell accommodation collection instrument set 132 Tapered surface part 135 First taper surface part (taper surface part)
136 2nd taper surface part LD axial direction

Claims (10)

A microtube comprising: a cylindrical container body having an opening formed at one end and a bottom closed at the other end; and a lid attached to the container body and sealing the opening. There,
The inner surface of the said bottom part of the said container main body is a microtube formed in a concave curved surface with a radius of 0.8 mm or less.   The inner surface of the container body includes a tapered surface portion that is continuous with the inner surface of the bottom portion and is inclined at a predetermined angle with respect to the axial direction of the container body, and the taper angle of the tapered surface portion with respect to the axial direction is 10 ° or less. The microtube according to claim 1.   The microtube according to claim 2, wherein a taper angle of the tapered surface portion with respect to the axial direction is 7.5 ° or less.   The microtube according to claim 2 or 3, wherein a taper angle of the tapered surface portion with respect to the axial direction is 5 ° or more.   The inner surface of the container main body further includes a second tapered surface portion that is continuous with the tapered surface portion and is inclined with respect to the axial direction of the container main body by an angle larger than the taper angle of the tapered surface portion. The microtube according to 1.   The microtube according to any one of claims 1 to 5, further comprising a leg portion extending from an outer surface of the container main body to the bottom side in the axial direction of the container main body.   The capacity | capacitance of the said container main body is 500 microliters-1500 microliters, The microtube in any one of Claims 1-6. The container body includes a male screw portion formed on the outer peripheral surface on the opening side,
The microtube according to any one of claims 1 to 7, wherein the lid is configured by a cap having a female screw portion that can be screwed to the male screw portion on an inner peripheral surface. The microtube according to any one of claims 1 to 8,
A tube connecting portion connectable to an upper portion of the container main body, and the tube connecting portion is connected to the container main body and extends along the axial direction of the container main body to enter the container main body through the opening. A cell containing and collecting instrument set comprising: a cell collecting auxiliary tool having a tubular portion that communicates with the cell collecting auxiliary tool set. A microtube comprising: a cylindrical container body having an opening formed at one end and a bottom closed at the other end; and a lid attached to the container body and sealing the opening. A cell recovery aid that assists in the recovery of cells attached and contained in the container body,
A tube connecting portion connectable to an upper portion of the container body;
A cell collection aid comprising: a tubular portion that extends along the axial direction of the container body and communicates with the inside of the container body through the opening in a state where the tube connection portion is connected to the container body.

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