JP2012050437A - Humanized antibody recognizing beta amyloid peptide - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide improved medicines and methods for treating diseases associated with amyloid deposition of Aβ (beta amyloid peptide) in a patient brain.SOLUTION: There is provided a humanized immunoglobulin light chain comprising (i) a variable region complementarity determining region (CDR) from a 12A11 immunoglobulin light chain variable region sequence shown as a specific sequence and (ii) a variable framework region from a human acceptor immunoglobulin light chain sequence. The preferable medicine comprises an antibody such as a humanized antibody.

Description

Related Application This application claims the benefit of previously filed US Provisional Application No. 60 / 474,654 (filed May 30, 2003), entitled “Humanized Antibody Recognizing Beta Amyloid Peptide”. The entire contents of the above-cited applications are incorporated herein by reference.

Alzheimer's disease (AD) is a progressive disease that results in senile dementia.
See Non-Patent Document 1, Patent Document 1, Non-Patent Document 2, Non-Patent Document 3, and Non-Patent Document 4. Broadly speaking, the disease falls into two categories: late-onset that occurs at older age (age 65 and above) and early-onset that occurs well before old age, ie, age 35-60. For both types of disease, the pathology is similar but is more severe and widespread when the abnormality begins early. The disease is characterized by at least two types of lesions in the brain: neurofibrillary tangles and senile plaques. Neurofibrillary tangles are intracellular depositions of microtubule-associated tau protein that consists of two fibers that wrap around each other in pairs. Senile plaques (ie, amyloid plaques) are areas of an amorphous network up to 150 μm in diameter with extracellular amyloid deposits in the center, which can be seen by microscopic analysis of brain tissue sections. Accumulation of amyloid plaques in the brain is also associated with Down syndrome and other cognitive impairments.

The main component of the plaque is a peptide called Aβ or β-amyloid peptide. A
The β peptide is a 39-43 amino acid 4 kDa internal fragment of a large transmembrane glycoprotein called amyloid precursor protein (APP). As a result of proteolytic processing of APP with various secretase enzymes, Aβ is mainly found in both a short form of 40 amino acids in length and a long form of 42 to 43 amino acids in length. A portion of the hydrophobic transmembrane domain of APP is found at the carboxy terminus of Aβ and may be responsible for the cause of Aβ's ability to aggregate in plaques, especially in the long form. Accumulation of amyloid plaques in the brain can lead to neuronal death. Physical symptoms associated with this form of nerve deterioration are characteristic of Alzheimer's disease.

Several mutations within the APP protein correlate with the presence of Alzheimer's disease. For example, Non-Patent Document 5 (valine ⁷¹⁷ to isoleucine), Non-Patent Document 6 (valine ⁷¹⁷ to glycine), Non-Patent Document 7 (valine ⁷¹⁷ to phenylalanine), Non-Patent Document 8 (
Double mutation, lysine ⁵⁹⁵ -methionine ⁵⁹⁶ to asparagine ⁵⁹⁵ -leucine ⁵
See change to ⁹⁶ ). Such mutations are thought to produce Alzheimer's disease by increasing or modifying the processing of APP to Aβ, particularly by processing APP to increased amounts of the long form of Aβ (ie, Aβ1-42 and Aβ1-43). Mutations in other genes, such as the presenilin gene, PS1 and PS2, are thought to indirectly affect APP processing to produce increased amounts of long APP (see Non-Patent Document 9).

The mouse model has been successfully used to determine the importance of amyloid plaques in Alzheimer's disease (Non-Patent Document 4 and Non-Patent Document 10 above). In particular, PDAPP transgenic mice (expressing a variant of human APP and developing Alzheimer's disease at a young age), when injected with the long form of Aβ, are associated with reduced progression of Alzheimer's disease and A
Both increase in antibody titer to β peptide are shown (Non-patent Document 11). The above observation results are
β, especially its long form, is a causative factor for Alzheimer's disease.
Hardy et al. , International Patent Publication No. 92/13069 Pamphlet Selkoe, TINS 16: 403 (1993) Selkoe, J .; Neuropathol. Eur. Neurol. 53: 438 (1994) Duff et al. , Nature 373: 476 (1995). Games et al. , Nature 373: 523 (1995). Goate et al. , Nature 349: 704 (1991). Charter Harlan et al. , Nature 353: 844 (1991). Murrell et al. , Science 254: 97 (1991). Mullan et al. , Nature Genet. 1: 345 (1992) Hardy, TINS 20: 154 (1997) Johnson-Wood et al. , Proc. Natl. Acad. Sci. USA 94: 1550 (1997) Schenk et al. , Nature 400, 173 (1999)

Thus, novel therapies and reagents for the treatment of Alzheimer's disease, particularly physiological (
There is a need for treatments and drugs that can benefit from treatment with (ie, non-toxic) administration.

The present invention relates to novel immunoglobulin reagents, specifically amyloid.
nic) featuring therapeutic antibody reagents for the prevention and treatment of diseases such as Alzheimer's disease. The present invention is based, at least in part, on the identification and characterization of 12A11, a monoclonal antibody that specifically binds to Aβ peptide and is effective in reducing plaque burden associated with amyloid disorders. The structure and function analysis of this antibody leads to the design of various humanized antibodies for use in prevention and / or therapy. In particular, the invention features the humanization of antibodies of various regions of the antibody, whereby humanized immunoglobulins or antibody chains, native humanized immunoglobulins or antibodies, and functional immunoglobulins or antibody fragments. In particular, antigen-binding fragments of characteristic antibodies are provided.

Polypeptides comprising the complementarity determining regions (CDRs) of the characteristic monoclonal antibodies are also disclosed as polynucleotide reagents, vectors and host cells suitable for encoding the polypeptides.

Methods for treating amyloid diseases or disorders (eg, Alzheimer's disease) are disclosed as pharmaceutical compositions and kits for use in such applications.

Further characterized is the substitution of the appropriate immunological functions and substitutions in the design of humanized antibodies with improved binding affinity and / or reduced immunogenicity when used as therapeutic reagents. It is a method for identifying residues within a characteristic monoclonal antibody that is important for identifying susceptible residues.

Further characterized are antibodies with altered effector functions (eg, humanized antibodies) and their use in therapeutic methods.

FIG. 1 illustrates the results from experiments testing the effectiveness of various antibodies, including 12A11, in clearing Aβ plaques in an ex vivo phagocytosis assay. FIG. 2A illustrates the results from experiments testing the effectiveness of various antibodies, including 12A11, at low total Aβ levels. Bars represent intermediate values and horizontal long dashed lines indicate control levels. FIG. 2B illustrates the results from experiments analyzing the effectiveness of various antibodies, including 12A11, in reducing neuritic dystrophy. Bars represent intermediate values and horizontal long dashed lines indicate control levels. Data are shown for individual animals and expressed as a percentage of neuritic dystrophy relative to the mean of controls (set to 100%). FIG. 3A shows the DNA sequence containing the murine 12A11 VL chain sequence and the deduced amino acid sequence for the VL chain (SEQ ID NOs: 5 and 2, respectively). The mature VL chain is indicated by a black thick bar. CDRs are indicated by white bars. FIG. 3B shows the DNA sequence containing the murine 12A11 VH chain sequence and the deduced amino acid sequence for the VH chain (SEQ ID NOs: 6 and 3, respectively). The mature VH chain is indicated by a black thick bar. CDRs are indicated by white bars. DNA sequences include cloning sites and Kozak sequences (upstream of the coding sequence) and splices and cloning sequences (downstream). FIG. 4 illustrates ELISA results from experiments measuring the binding of chimeric 12A11, chimeric and humanized 3D6, and chimeric and humanized 12B4 to Aβ 1-42. Figures 5A-B show murine (or chimeric) 12A11 (SEQ ID NO: 2), humanized 12A11 (mature peptide, SEQ ID NO: 7), GeneBank BAC01733 (SEQ ID NO: 8), and germline A19 (X63397, SEQ ID NO: 9) Alignment of amino acid sequences of antibody light chains. CDR regions are boxed. Filled residues are underlined. Numbered from primary methionine rather than Kabat numbering. FIG. 5B shows murine (or chimeric) 12A11 (SEQ ID NO: 4), humanized 12A11 (version 1) (mature peptide, SEQ ID NO: 10), gene bank AAA69734 (SEQ ID NO: 11), and germline gene bank 567123 antibody ( The alignment of the amino acid sequence of the heavy chain of SEQ ID NO: 12) is shown. Filled residues are underlined, canonical residues are shown as solid lines, and vernier residues are shown as dots. Numbered from primary methionine rather than Kabat numbering. (Continuation of FIG. 5A) 6A-B show humanized 12A11v1 (SEQ ID NO: 10), v2 (SEQ ID NO: 3), v2.1 (SEQ ID NO: 14), v3 (SEQ ID NO: 15), v4.1 (SEQ ID NO: 6), v4.2. (SEQ ID NO: 17), v4.3 (SEQ ID NO: 18), v4.4 (SEQ ID NO: 19), v5.1 (SEQ ID NO: 20), v5.2 (SEQ ID NO: 21), v5.3 (SEQ ID NO: 22) , V5.4 (SEQ ID NO: 23), v5.5 (SEQ ID NO: 24), v5.6 (SEQ ID NO: 25), v6.1 (SEQ ID NO: 26), v6.2 (SEQ ID NO: 27), v6.3 ( The alignment of the heavy chain amino acid sequences of SEQ ID NO: 28), v6.4 (SEQ ID NO: 29), v7 (SEQ ID NO: 30) and v8 (SEQ ID NO: 31) is shown. (Continued from FIG. 6A) FIG. 6C shows the back mutations that occurred within humanized 12A11v1-v8. FIG. 7 shows results from an aggregated Aβ (1-42) ELISA comparing chimeric 12A11, humanized 12A11v1, humanized 12A11v2, humanized 12A11v2.1, and humanized 12A11v3. FIG. 8 shows the results of a competitive Aβ1-42 ELISA binding assay comparing murine 12A11, chimeric 12A11 and h12A11v1.

Detailed Description of the Invention The present invention features novel immunological reagents and methods for the prevention or treatment of Alzheimer's disease or other amyloid diseases. The present invention at least in part binds beta amyloid protein (Aβ) (eg, binds soluble and / or aggregated Aβ), mediates phagocytosis (eg, of aggregated Aβ), reduces plaque burden and / or neuritis Based on characterization of 12A11, a monoclonal immunoglobulin that is effective in reducing sex dystrophy (eg, in patients). The present invention is further based on the determination and structural characterization of the primary and secondary structures of the variable light and heavy chains of 12A11 immunoglobulin and the identification of residues important for activity and immunogenicity.

The immunoglobulin is characterized by comprising a variable light chain and / or variable heavy chain of the 12A11 monoclonal immunoglobulin described herein. Preferred immunoglobulins, such as therapeutic immunoglobulins, are characterized by comprising a humanized variable light chain and / or a humanized variable heavy chain. Preferred variable light and / or variable heavy chains are 12A11 immunoglobulins (
Complementarity determining regions (CDRs) from, eg, donor immunoglobulins) and variable framework regions from or essentially from human acceptors. The phrase “essentially from human acceptor immunoglobulin” is selected to improve the activity of the humanized immunoglobulin, although most or important framework residues are derived from human acceptor sequences ( Allow substitution of residues at certain positions with residues that have been modified (e.g., modified to mimic the activity of a donor immunoglobulin) or selected to reduce the immunogenicity of a humanized immunoglobulin Means that.

In one aspect, the invention includes a 12A11 variable region complementarity determining region (CDR) (ie, includes 1, 2 or 3 CDRs from the light chain variable region sequence shown as SEQ ID NO: 2 or Including one, two or three CDRs from the heavy chain variable region sequence shown as number 4), and a variable framework region from the human acceptor immunoglobulin light chain or heavy chain sequence, optionally corresponding murine Having at least one of the framework residues backmutated to a residue, wherein the backmutation specifies Aβ binding (d
It is characterized by a humanized immunoglobulin light or heavy chain that does not substantially affect the ability of the chain to direct.

In one aspect, the invention includes a 12A11 variable region complementarity determining region (CDR) (ie, includes 1, 2 or 3 CDRs from the light chain variable region sequence shown as SEQ ID NO: 2 or Comprising one, two or three CDRs from the heavy chain variable region sequence designated as number 4), and essentially comprising a variable framework region from the human acceptor immunoglobulin light or heavy chain sequence, optionally Having at least one of the framework residues backmutated to the corresponding murine residue, wherein the backmutation does not essentially affect the ability of the chain to specify Aβ binding, Characterized by a modified immunoglobulin light chain or heavy chain.

In another aspect, the invention includes a 12A11 variable region complementarity determining region (CDR) (eg, including 1, 2 or 3 CDRs from the light chain variable region sequence shown as SEQ ID NO: 2 and / or Or comprises 1, 2 or 3 CDRs from the heavy chain variable region sequence shown as SEQ ID NO: 4), and essentially comprises a variable framework region from the human acceptor immunoglobulin light or heavy chain sequence; Optionally having at least one framework residue substituted with the corresponding amino acid residue from the murine 12A11 light chain or heavy chain variable region sequence, wherein the framework residue comprises (a) the antigen directly Non-covalently bound residues, (b) residues adjacent to a CDR, (c) residues that interact with a CDR (eg, elucidation of homologous known immunoglobulin chains) A humanized immunoglobulin light chain or heavy selected from the group consisting of residues involved in the VL-VH interface; and Characterized by chains.

In another aspect, the invention includes a variable framework region from a 12A11 variable region complementarity determining region (CDR) and a human acceptor immunoglobulin light or heavy chain sequence, optionally mouse 12A11 light or heavy chain variable. Having at least one framework residue substituted with the corresponding amino acid residue from the region sequence, wherein the framework residue is:
Residues that can affect the light chain variable region conformation or function identified by analysis of a three-dimensional model of the variable region, such as residues that can interact with the antigen, residues proximal to the antigen binding site, C
Residues that can interact with DR, residues adjacent to CDRs, residues within 6 cm of CDR residues, canonical residues, vernier zone residues, interchain filling residues, unusual residues, or structural models It is characterized by a humanized immunoglobulin light or heavy chain, a glycosylation site residue on the surface.

In another aspect, the invention features substitution of at least one rare human framework residue in addition to the substitutions described above. For example, a rare residue can be substituted with an amino acid residue that is common to human variable chain sequences at that position. Alternatively, rare residues can be substituted with corresponding amino acid residues from homologous germline variable chain sequences.

In another aspect, the invention features a humanized immunoglobulin comprising light and heavy chains as described above, or antigen-binding fragments of the immunoglobulin. In an exemplary embodiment, the humanized immunoglobulin is at least 10 ⁷ M against beta amyloid peptide (Aβ).
It binds (eg, specifically binds) with a binding affinity of ⁻¹ , 10 ⁸ M ⁻¹ or 10 ⁹ M ⁻¹ . In another aspect, the immunoglobulin or antigen-binding fragment is isotype γ.
A heavy chain with 1 is included. In another aspect, the immunoglobulin or antigen-binding fragment is
Bind to one or both of soluble beta amyloid peptide (Aβ) and aggregated Aβ (
For example, specific binding). In another aspect, the immunoglobulin or antigen-binding fragment captures soluble Aβ (eg, soluble Aβ1-42). In another embodiment, the immunoglobulin or antigen-binding fragment mediates phagocytosis (eg, induces phagocytosis) of beta amyloid peptide (Aβ). In yet another aspect, the immunoglobulin or antigen-binding fragment crosses the blood brain barrier in the subject. In yet another aspect, the immunoglobulin or antigen-binding fragment reduces either or both beta amyloid peptide (Aβ) load and neuritis dystrophy in the subject.

In another aspect, the invention features a chimeric immunoglobulin comprising a 12A11 variable region (eg, a variable region sequence shown as SEQ ID NO: 2 or SEQ ID NO: 4). In yet another aspect,
The immunoglobulin, or antigen-binding fragment thereof, further comprises a constant region from IgG1.

The immunoglobulins described herein are particularly suitable for use in therapeutic methods aimed at the prevention or treatment of amyloid diseases. In one aspect, the invention features a method of prevention or treatment of amyloid disease (eg, Alzheimer's disease) comprising administering to a patient an effective dose of a humanized immunoglobulin described herein. In another aspect, the invention features a pharmaceutical composition comprising a humanized immunoglobulin described herein and a pharmaceutical carrier. Isolated nucleic acid molecules, vectors and host cells for producing immunoglobulins or immunoglobulin fragments or chains as described herein, and for producing immunoglobulin immunoglobulin fragments or immunoglobulin chains as described above A method is also featured.

The invention further features a method of identifying 12A11 residues that are each likely to be substituted when producing humanized 12A11 immunoglobulins, respectively. For example, a method for identifying variable framework region residues that are prone to substitution can be found in 12A on elucidated homologous immunoglobulin structures.
To model the three-dimensional structure of 11 variable regions and identify residues that are prone to substitution:
Analyzing the model of residues capable of affecting 12A11 immunoglobulin variable region conformation or function. The present invention further provides SEQ ID NO: 2 in generating a three-dimensional image of a 12A11 immunoglobulin, 12A11 immunoglobulin chain, or domain thereof.
Or is characterized by the use of the variable region sequence shown as SEQ ID NO: 4, or any part thereof.

The invention further features an immunoglobulin having an altered effector function that is capable of binding an effector molecule, such as a complement or receptor on effector cells. In particular, the immunoglobulins of the present invention have a modified constant region, eg, an Fc region, wherein at least one amino acid residue in the Fc region is replaced with a different residue or side chain. In one embodiment, the modified immunoglobulin is of the IgG class, such as at least one amino acid residue substitution in the Fc region such that the immunoglobulin has an altered effector function compared to a non-denatured immunoglobulin. Comprising. In particular embodiments, the immunoglobulins of the present invention have altered effector functions that are even less immunogenic (eg, do not cause unwanted effector cell activity, lysis, or complement binding).
Or have improved amyloid removal and / or have a desirable half-life.

Before describing the invention, it is helpful to provide definitions of some terms that will be used in the future.

The term “immunoglobulin” or “antibody” (used interchangeably herein)
Refers to a protein having a basic four-polypeptide chain structure consisting of two heavy chains and two light chains, which chains are stabilized, for example, by interchain disulfide bonds, which specifically bind antigens Have the ability. The term “single-chain immunoglobulin” or “single-chain antibody” (used interchangeably herein) refers to a protein having a two-polypeptide chain structure consisting of a heavy chain and a light chain, The chain is stabilized, for example, by interchain disulfide bonds, which have the ability to specifically bind antigen. The term “domain” is a globular form of a heavy or light chain polypeptide comprising, for example, a β-pleat sheet and / or a peptide loop stabilized by an intrachain disulfide bond (eg comprising a 3-4 peptide loop). Refers to an area. Domains are further referred to herein as relative absence of sequence variations within the domains of the various class members in the case of “constant” domains, or within the domains of the various class members in the case of “variable” domains. Based on the significant fluctuations of, it is referred to herein as “steady” or “variable”. Antibody or polypeptide “domains” are often referred to interchangeably in the art as antibody or polypeptide “regions”. The “constant” domains of antibody light chains are referred to interchangeably as “light chain constant regions”, “light chain constant domains”, “CL” regions or “CL” domains. The “constant” domains of antibody heavy chains are referred to interchangeably as “heavy chain constant regions”, “heavy chain constant domains”, “CH” regions or “CH” domains. The “variable” domains of antibody light chains are referred to interchangeably as “light chain variable regions”, “light chain variable domains”, “VL” regions or “VL” domains. The “variable” domains of antibody heavy chains are referred to interchangeably as “heavy chain constant regions”, “heavy chain constant domains”, “VH” regions or “VH” domains.

The term “region” refers to part or part of an antibody chain or antibody chain domain (eg part or part of a heavy or light chain or part or part of a constant or variable domain as defined herein), As well as further subdivided parts or portions of the chains or domains described above. For example, light chains and heavy chains or light chain or heavy chain variable domains are interspersed within “framework regions” or “FRs” as defined herein, “complementarity determining regions” or “CDRs”. "including.

Immunoglobulins or antibodies can exist as monomeric or polymeric forms, such as IgM antibodies present in pentamers and / or IgA antibodies present in monomers, dimers or multimers. The term “fragment” refers to a portion or part of an antibody or antibody chain comprising fewer amino acid residues than the original or complete antibody or antibody chain. Fragments can be obtained through chemical or enzymatic treatment of the original or complete antibody or antibody chain. Fragments can also be obtained by recombinant means. An exemplary fragment is F
Includes ab, Fab ′, F (ab ′) 2 and / or Fv fragments. The term “antigen-binding fragment” refers to an immunoglobulin or polypeptide fragment of an antibody that binds antigen or competes for antigen binding (ie, specific binding) with the original antibody (eg, the original antibody from which they were derived). Point to.

The term “conformation” refers to the tertiary structure of a protein or polypeptide (eg, antibody, antibody chain, domain or region thereof). For example, the phrase “light (or heavy) chain conformation” refers to the tertiary structure of the light (or heavy) chain variable region, and the phrase “antibody conformation” or “antibody fragment conformation” Refers to the tertiary structure of an antibody or fragment thereof.

“Specific binding” of an antibody means that the antibody exhibits an easily perceivable affinity for a particular antigen or epitope, and generally does not exhibit significant cross-reactivity. In exemplary embodiments, the antibody does not exhibit cross-reactivity (eg, does not cross-react with non-Aβ peptides or with distant epitopes on Aβ). “Applicable” or preferred binding is at least 10 ⁶ , 10 ⁷ , 10 ⁸ , 10 ⁹ M ⁻¹ , or 10 ¹⁰ M ^−.
Includes binding with an affinity of ¹ . Affinities greater than 10 ⁷ M ⁻¹ , preferably greater than 10 ⁸ M ⁻¹ are even more preferred. Those intermediate values shown herein are also intended to be within the scope of the invention and preferred binding affinities are, for example, 10 ⁶ to 10 ¹⁰ M ⁻¹ , preferably 10 ⁷ to 10 ¹⁰ M ⁻¹ , More preferably, the affinity range of 10 ⁸ to 10 ¹⁰ M ⁻¹ can be indicated. An antibody that does not show significant cross-reactivity is an undesirable entity
(Eg, undesired proteinaceous objects) that cannot be easily detected. For example, an antibody that specifically binds Aβ binds appreciably but does not react significantly with non-Aβ proteins or peptides (eg, non-Aβ proteins or peptides contained in plaques). Antibodies specific for a particular epitope do not significantly cross-react with distant epitopes on, for example, the same protein or peptide. Specific binding can be determined by any means recognized in the art for determining such binding. Preferably, specific binding is determined by Scatchard analysis and / or competitive binding assays.

Binding fragments are produced by recombinant DNA techniques or by enzymatic or chemical cleavage of the native immunoglobulin. Binding fragments include Fab, Fab ′,
F (ab ′) ₂ , Fabc, Fv, single chain, and single chain antibodies are included. In addition to “bispecific” or “bifunctional” immunoglobulins or antibodies, it is understood that each immunoglobulin or antibody has the same binding site. A “bispecific” or “bifunctional antibody” is an artificial hybrid antibody having two different heavy / light chain pairs and two different binding sites. Bispecific antibodies can be produced by a variety of methods including fusion of hybridomas or linking of Fab ′ fragments. For example, Songsivillai & Lac
hmann, Clin. Exp. Immunol. 79: 315-321 (1990);
Kostelny et al. , J .; Immunol. 148, 1547-1553 (
(1992).

The term “humanized immunoglobulin” or “humanized antibody” refers to an immunoglobulin or antibody comprising at least one humanized immunoglobulin or antibody chain (ie, at least one humanized light or heavy chain). “Humanized immunoglobulin chain” or “humanized antibody chain” (
That is, the terms “humanized immunoglobulin light chain” or “humanized immunoglobulin heavy chain”) are essentially variable framework regions from human immunoglobulins or antibodies and complements from essentially non-human immunoglobulins or antibodies. Sex determining region (CDR) (eg at least 1
A region comprising one CDR, preferably two CDRs, more preferably three CDRs), and further a constant region (eg in the case of light chains at least one constant region or part thereof)
And in the case of the heavy chain, preferably an immunoglobulin or antibody chain containing three constant regions) (
Ie light chain or heavy chain respectively). The term “humanized variable region” (eg, “humanized light chain variable region” and “humanized heavy chain variable region”) is essentially a variable framework region from a human immunoglobulin or antibody and essentially non-human. Refers to the variable region that includes complementarity determining regions (CDRs) from immunoglobulins or antibodies.

The phrase “essentially from a human immunoglobulin or antibody” or “essentially human” refers to a region when aligned to a human immunoglobulin or antibody amino sequence for comparison purposes,
At least 80-90%, 90-95% relative to human framework or constant region sequences
Or share 95-99% identity (ie local sequence identity), eg conservative substitutions,
It means to allow consensus sequence substitution, germline substitution, back mutation and the like. Introduction of conservative substitutions, consensus sequence substitutions, germline substitutions, backmutations, etc. is referred to as “optimization” of, for example, a humanized antibody or chain. The phrase “essentially from non-human immunoglobulin or antibody” or “essentially non-human” refers to at least 80-95%, preferably at least 90-95% relative to that of a non-human organism, such as a non-human animal. More preferably, it means having an immunoglobulin or antibody sequence that is 96%, 97%, 98%, or 99% identical.

Thus, all regions or residues of a humanized immunoglobulin or antibody, or humanized immunoglobulin or antibody chain, possibly except for CDRs, correspond to corresponding regions of one or more native human immunoglobulin sequences Essentially identical to the residue. The term “corresponding region” or “corresponding residue” is the same as the region or residue on the first amino acid or nucleotide sequence when the first and second sequences are optimally arranged for comparison purposes. Refers to a region or residue on the second amino acid or nucleotide sequence that occupies a position (ie equivalent).

The term “significant identity” means at least 50-60% sequence identity, preferably at least 60-70% sequence identity, for example when optimally aligned by the program GAP or BESTFIT using default gap weights, More preferably at least 70-80% sequence identity, more preferably at least 80-90% sequence identity, even more preferably at least 90-95% sequence identity, and even more preferably at least 95% or more. By two polypeptide sequences that share sequence identity or more (eg 99% sequence identity or more) is meant. The term “essentially identity” means, for example, a program GAP or BEST using default gap weights.
When aligned by FIT, at least 80-90% sequence identity, preferably at least 90-95% sequence identity, and more preferably at least 95% sequence identity or more (eg, 99% sequence identity) Means two polypeptide sequences that share (identity or more). For sequence comparison, typically one sequence is referenced.
nce) serves as a sequence to which the test sequence is compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the designated program parameters.

Optimal alignment of sequences for comparison can be found, for example, in Smith & Waterman, Adv.
Appl. Math. 2: 482 (1981) by the local homology algorithm.
dlman & Wunsch, J.M. Mol. Biol. 48: 443 (1970), according to Pearson & Lipman, Proc. Nat '
l. Acad. Sci. USA 85: 2444 (1988), by means of similarity search, computerized means of those algorithms (GAP, BESTFIT, FASTA, and TF)
ASTA, Wisconsin Genetics Software Package
, Genetics Computer Group, 575 Science Dr.
, Madison, WI) or by visual inspection (generally Ausubel)
et al. , Current Protocols in Molecular Bi
(see logy). One example of an algorithm that is suitable for determining percent sequence identity and sequence similarity is the BLAST algorithm, which is Altschul.
et al. , J .; Mol. Biol. 215: 403 (1990). The software for performing BLAST analysis is National Center.
Publicly available through for Biotechnology Information (available publicly via the National Institute of Health NCBI Internet server). Typically, default program parameters can be used to perform sequence comparisons, but proprietary parameters can also be used. For amino acid sequences, the BLASTP program defaults to 3 word lengths (W), 1
Expectation of zero (E), and use BLOSUM62 scoring matrix (Hen
ikoff & Henikoff, Proc. Nat'l. Acad. Sci. USA
89: 10915 (1989)).

Preferably, non-identical residue positions differ by conservative amino acid substitutions. For the purpose of classifying amino acid substitutions as conservative and non-conservative, amino acids are classified as follows: Group I (hydrophobic side chains): leu, met, ala, va
l, leu, ile; Group II (neutral hydrophilic side chain): cys, ser, thr; Group III (acidic side chain): asp, glu; Group IV (basic side chain): asn, gl
n, his, lys, arg; Group V (residues affecting the chain direction): gly, pro;
And Group VI (aromatic side chain): trp, tyr, phe. Conservative substitutions include substitutions between amino acids within the same class. A non-conservative substitution is an exchange from one member of those classes to another member.

Preferably, the humanized immunoglobulin or antibody binds the antigen with an affinity within a factor of 3, 4, or 5 than that of the corresponding non-humanized antibody. For example, non-humanized antibody is 10 ⁹
A humanized antibody has a binding affinity of M ⁻¹ of at least 3 × 10 ⁹ M ⁻¹ , 4 × 10
It will have a binding affinity of ⁹ M ⁻¹ or 5 × 10 ⁹ M ⁻¹ . When describing the binding of an immunoglobulin or antibody chain, the chain can be described based on its ability to “direct antigen (eg, Aβ) binding”. A chain is said to be “direct antigen binding” if it provides specific binding or binding affinity for the native immunoglobulin or antibody (or antigen binding fragment thereof). An antibody (or antigen-binding fragment thereof) comprising an equivalent chain in which the binding affinity of the original immunoglobulin or antibody (or antigen-binding fragment thereof) comprising a heavy or light chain lacks a mutation A mutation (eg, backmutation) essentially affects the ability of a heavy or light chain to specify antigen binding when affected (eg, reduced) by at least an order of magnitude compared to Called to affect. The binding affinity of the original immunoglobulin or antibody (or antigen-binding fragment thereof) comprising the chain,
When the mutation affects (eg, decreases) only a factor of 2, 3, or 4 as compared to that of an antibody (or antigen-binding fragment thereof) comprising an equivalent chain that lacks the mutation, the Mutations are referred to as “essentially not affecting (eg, reducing)” the ability of the chain to specify antigen binding.

The term “chimeric immunoglobulin” or antibody refers to an immunoglobulin or antibody in which the variable region is derived from a first species and the constant region is derived from a second species. Chimeric immunoglobulins or antibodies can be constructed from immunoglobulin gene segments belonging to different species, for example, by genetic engineering. The term “humanized immunoglobulin” or “humanized antibody” is not intended to encompass chimeric immunoglobulins or antibodies, as defined below. Humanized immunoglobulins or antibodies are chimeric in their structure (ie, comprise regions from more than one protein), but they are additional molecules that are not found within the chimeric immunoglobulins or antibodies defined herein. Features (ie, variable regions comprising donor CDR residues and acceptor framework residues).

An “antigen” is an object (eg, a proteinaceous thing or peptide) to which an antibody specifically binds.

The term “epitope” or “antigenic determinant” refers to a site on an antigen to which an immunoglobulin or antibody (or antigen-binding fragment thereof) specifically binds. An epitope can be formed from both contiguous amino acids or non-contiguous amino acids that are juxtaposed by tertiary folding of the protein. Epitopes formed from consecutive amino acids are typically preserved when exposed to denaturing solvents, whereas epitopes formed by tertiary folding are typically lost upon treatment with denaturing solvents. The epitope is typically at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 1 within a single conformation.
Contains 5 amino acids. Methods for determining the conformation of an epitope include, for example, X-ray crystallography and two-dimensional nuclear magnetic resonance. For example Epitope Mappi
ng Protocols in Methods in Molecular Bio
log, Vol. 66, G.M. E. See Morris, Ed (1996).

Antibodies that recognize the same epitope can be identified by a simple immunoassay that demonstrates the ability of one antibody to block the binding of another antibody to a target antigen, ie, a competitive binding assay. Competitive binding is determined in an assay in which the test immunoglobulin inhibits the specific binding of a reference antibody to a common antigen, such as Aβ. Many types of competitive binding assays are known, for example: solid phase direct or indirect radioimmunoassay (RIA), solid phase direct or indirect enzyme immunoassay (EIA), sandwich type competitive assay (Stahli et al
. , Methods in Enzymology 9: 242 (1983)); solid phase direct biotin-avidin EIA (Kirkland et al., J. Immuno.
l. 137: 3614 (1986)); solid phase direct labeling assay, solid phase direct labeling sandwich assay (Harlow and Lane, Antibodies: A La
borory Manual, Cold Spring Harbor Press
(1988)); solid phase direct labeling RIA (Morel et al.) Using I-125 labeling.
al. Mol. Immunol. 25 (1): 7 (1988)); solid phase direct biotin-avidin EIA (Cheung et al., Virology 176: 54).
6 (1990)); and direct labeling RIA (Moldenhauer et al., S
cand. J. et al. Immunol. 32:77 (1990)). Typically, such assays involve the use of purified antigen bound to a solid surface or cells having any of them, unlabeled test immunoglobulin and labeled reference immunoglobulin. Competitive inhibition is measured by determining the amount of label that binds to the solid surface or cells in the presence of the test immunoglobulin. Usually the test immunoglobulin is present in excess. Usually when there is an excess of competing antibodies,
It has at least 50-55%, 55-60% specific binding of the reference antibody to the common antigen,
Inhibits 60-65%, 65-70%, 70-75% or more.

Epitopes are also recognized by immunological cells such as B cells and / or T cells. Epitope cell recognition is due to ³ H thymidine incorporation, cytokine secretion,
It can be determined by antibody secretion or by an in vitro assay that measures antigen-dependent proliferation as determined by antigen-dependent killing (cytotoxic T lymphocyte assay).

Exemplary epitopes or antigenic determinants are human amyloid precursor protein (APP)
But are preferably found within the Aβ peptide of APP. APP isoforms, such as APP ⁶⁹⁵ APP ⁷⁵¹ and APP ^7
70 exists. Amino acids within APP are assigned numbers according to the sequence of the APP ⁷⁷⁰ isoform (see, eg, Genebank Accession Number P05067).
Aβ (also referred to herein as beta amyloid peptide and Abeta) peptide is an approximately 4-kDa internal fragment of 39-44 amino acids of APP (Aβ39, Aβ40, Aβ41, Aβ42 and Aβ43). For example, Aβ40 is residue 67 of APP
Aβ42 consists of residues 673-713 of APP. in vi
As a result of proteolytic treatment of APP with various secretase enzymes in vivo or in situ, Aβ is a “short form” of 40 amino acids in length and an amino acid length of 42˜
Both “long” types in the 43 range were found. Preferred epitopes or antigenic determinants described herein are located within the N-terminus of the Aβ peptide and are amino acids 1-1 of Aβ.
Within 0, preferably residues 1-3, 1-4, 1-5, 1-6, 1-7 or 3-7 of Aβ42
Including residues from Additional reference epitopes or antigenic determinants are Aβ residues 2-4, 5,
6, 7 or 8, residues 3-5, 6, 7, 8, or 9 of Aβ, or residue 4 of Aβ42
-7, 8, 9 or 10. The antigen is an epitope within a particular residue, eg Aβ 3-7
The meaning of a particular residue (ie Aβ3-7 in this example)
The antibody specifically binds to a polypeptide comprising Such antibodies need not necessarily contact each residue in Aβ3-7. Also, any single amino acid substitution or deletion within Aβ3-7 does not necessarily significantly affect binding affinity.

The term “amyloid disease” includes any disease associated with (or resulting from) the formation or deposition of insoluble amyloid fibrils. Exemplary amyloid diseases include systemic amyloidosis, Alzheimer's disease, adult-onset diabetes, Parkinson's disease, Huntington's disease,
Including but not limited to anterior temporal dementia and prion-associated infectious spongiform encephalopathy (Cool and Creutzfeldt-Jakob disease in humans and sheep spongiform encephalopathy and BSE in sheep and cattle, respectively). Various amyloid diseases are defined or characterized by the nature of the polypeptide component of the deposited fibrils. For example, within a subject or patient with Alzheimer's disease, β-amyloid protein (eg, wild type, variant, or truncated β-amyloid protein) has been characterized as a polypeptide component of amyloid deposition. Thus, Alzheimer's disease can be associated with, for example, “A in the subject's or patient's brain.
Examples of “diseases characterized by β deposition” or “diseases associated with Aβ deposition”. "
“β-amyloid protein”, “β-amyloid peptide”, “β-amyloid”, “A
The terms “β” and “Aβ peptide” are used interchangeably herein.

An “immunogenic agent” or “immunogen” can elicit an immunological response to itself when administered to a mammal, optionally with an adjuvant.

As used herein, the term “treatment” refers to the application or administration of a therapeutic agent to a patient, cure, cure, alleviate, release, modify, treat, alleviate a disease, symptom of the disease, or predisposition to the disease.
Defined as the application or administration of a therapeutic agent to a tissue or cell line isolated from a patient having a disease, disease symptoms or predisposition to a disease for the purpose of salvage, improvement, amelioration or influence.

The term “effective dose” or “effective dose” is defined as an amount sufficient to achieve or at least partially achieve the desired effect. The term “therapeutically effective dose” is defined as an amount sufficient to cure or at least partially block the disease and its complications in a patient already suffering from the disease. Effective amounts for this use depend on the severity of the infection and the general condition of the patient's own immune system.

The term “patient” includes human and other mammalian subjects that receive either prophylactic or therapeutic treatment.

“Soluble” or “degraded” Aβ includes monomeric soluble as well as oligomeric and soluble Aβ polypeptides (eg, soluble Aβ dimers, trimers, etc.) and refers to non-aggregated or isolated Aβ polypeptides. . “Insoluble” Aβ refers to aggregated Aβ polypeptides held together, for example, by non-covalent bonds. Aβ (eg, Aβ42) is believed to at least partially aggregate due to the presence of a hydrophobic residue (part of the transmembrane domain of APP) at the C-terminus of the peptide. Soluble Aβ can be found in vivo in biological fluids such as cerebrospinal fluid and / or serum. Alternatively, soluble Aβ can be prepared by dissolving lyophilized peptides by sonication in clean DMSO. The resulting solution is centrifuged (eg, 14,000 × g, 4 ° C., 10 minutes) to remove all insoluble particles.

The term “effector function” refers to effector activity present in the Fc region of an antibody (eg, an IgG antibody) and, for example, effector molecules such as complement and / or Fc.
Including the ability of an antibody to bind a receptor, it can control several immune functions of the antibody, such as effector cell activity, lysis, complement-mediated activity, antibody removal, and antibody half-life.

The term “effector molecule” refers to a molecule capable of binding to the Fc region of an antibody (eg, an IgG antibody), including but not limited to complement proteins or Fc receptors.

The term “effector cell” refers to an antibody (eg, IgG, typically via Fc receptors expressed on the surface of effector cells including, but not limited to, lymphocytes such as cells in which antigen is present and T cells. Antibody) refers to a cell capable of binding to the Fc portion of the antibody.

The term “Fc region” refers to the C-terminal region of an IgG antibody, in particular the C— of the heavy chain of the IgG antibody.
Refers to the terminal region. While the boundaries of the Fc region of an IgG heavy chain may vary slightly,
Typically defined as spanning from the amino acid residue Cys226 vicinity of the IgG heavy chain to the carboxyl terminus.

The term “Kabat numbering” is used unless otherwise stated.
al. ("Immunologically Related Protein Sequences", Fifth Edition, Public Health
h Service, National Institute of
Health, Bethesda, Md (1991), specifically incorporated herein by reference) is defined as the numbering of residues within an IgG heavy chain antibody, for example.

The terms “Fc receptor” or “FcR” refer to a receptor that binds to the Fc region of an antibody.
Exemplary Fc receptors that bind to the Fc region of antibodies (eg, IgG antibodies) are FcγRI, F
including but not limited to cγRII and FcγRIII subclass receptors,
Includes allelic variants and alternate spliced forms of their receptors. Fc receptors are described in Ravetch and Kinet, Annu. Rev. Immunol. 9:
457-92 (1991); Capel et al. , Immunomethods
4: 25-34 (1994) and de Haas et al. , J .; Lab. Cli
n. Med. 126: 330-41 (1995).
I. Immunology and therapeutic reagents The immunology and therapeutic reagents of the present invention comprise or consist of immunogens or antibodies, or functional or antigen-binding fragments thereof, as defined herein. The basic antibody structural unit is known to comprise a tetramer of subunits. Each tetramer consists of two identical pairs of polypeptide chains, each pair having one “light” (about 25 kDa) and one “heavy” (about 50-70 kDa) chain. The amino-terminal portion of each chain contains a variable region of about 100-110 or more amino acids that primarily corresponds to antigen recognition. The carboxy-terminal portion of each chain canonically primarily constant regions corresponding to effector functions.

Light chains are classified as either kappa or lambda and are approximately 230 residues in length. Heavy chains are classified as gamma (γ), mu (μ), alpha (α), delta (δ), or epsilon (ε), are approximately 450-600 residues in length, and are each IgG,
The antibody isotype is canonicalized as IgM, IgA, IgD and IgE. Both heavy and light chains are folded into domains. The term “domain” refers to a globular region of a protein, such as an immunoglobulin or an antibody. The immunoglobulin or antibody domain is
For example, it contains 3 to 4 peptide loops stabilized by β-pleated sheets and interchain disulfide bonds. A natural light chain has, for example, two domains (V _L and C _L ),
The original heavy chain is, for example, 4 to 5 domains (V _H , C _H 1, C _H 2 and C _H 3).
Have

Within the light and heavy chains, the variable and constant regions are “J” of about 12 or more amino acids.
Where the heavy chain also includes a “D” region of about 10 or more amino acids (generally, Fundamental Immunology, Paul, W.,
ed. , 2nd. ed. Raven Press, N.M. Y. (1989), Ch7), incorporated by reference in its entirety for all purposes).

The variable regions of each light / heavy chain pair form an antibody binding site. Thus, the original antibody has two binding sites. Except for bispecific or bispecific antibodies, the two binding sites are identical. The chains show all similar general structures of the three hypervariable regions, the so-called complementarity determining regions or relatively conserved framework regions (FR) linked by CDRs. Naturally occurring or recombinantly produced strands can also be expressed with a leader sequence that is removed during cell processing to produce the mature strand. For example, mature strands with non-naturally occurring leader sequences to facilitate modification of the secretion or processing of the particular strand of interest can be produced recombinantly.

The CDRs of the two mature strands of each pair are aligned by the framework region to allow binding to a specific epitope. From the N-terminus to the C-terminus, both the light and heavy chains comprise the domains FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4. “FR4” is also referred to in the art as the variable heavy chain D / J region and the variable light chain J region. The assignment of amino acids to each domain is Kabat, Sequence.
s of Proteins of Immunological Interest (
National Institute of Health, Bethesda, MD
1987 and 1991). Another structure definition is Chothia et
al. , J .; Mol. Biol. 196: 901 (1987); Nature 342
: 878 (1987); Mol. Biol. 186: 651 (1989) (hereinafter collectively referred to as “Chothia et al.” And incorporated by reference in its entirety for all purposes).
A. Aβ Antibodies The therapeutic agents of the present invention include antibodies that specifically bind to Aβ or other components of amyloid plaques. A preferred antibody is a monoclonal antibody. Some such antibodies specifically bind to the aggregated form of Aβ without binding to the soluble form. Some bind specifically to the soluble form without binding to the aggregated form. Some bind to both aggregated and soluble forms. The antibody used in the therapeutic method preferably has a native constant region, or at least a constant region sufficient to interact with an Fc receptor. Preferred antibodies are those that are effective in stimulating Fc-mediated phagocytosis of Aβ in the plaque. Human isotype IgG1 is preferred because it has the highest affinity of human isotype for FcRI receptors on phagocytic cells (eg on brain-resident macrophages or microglia). Human IgG1 is equivalent to murine IgG2a, so the latter is suitable for testing in vivo efficacy in an animal (eg mouse) model of Alzheimer's disease. Bispecific Fab fragments can also be used, in which one arm of the antibody has specificity for Aβ and the other has specificity for the Fc receptor. Preferred antibodies bind Aβ with a binding affinity greater than (or equal to) about 10 ⁶ , 10 ⁷ , 10 ⁸ , 10 ⁹ , or 10 ¹⁰ M ⁻¹ (including intermediate affinities of those values).

Monoclonal antibodies bind to specific epitopes within Aβ that can be conformational or non-conformational epitopes. The prophylactic and therapeutic efficacy of antibodies can be tested using the transgenic animal model procedures described in the Examples. Preferred monoclonal antibodies bind to an epitope within residues 1-10 of Aβ (designating the first N-terminal residue of the original Aβ as 1), more preferably to an epitope within residues 3-7 of Aβ. To do. In some methods, multiple monoclonal antibodies with binding specificities for different epitopes are used, for example, antibodies specific for epitopes within residues 3-7 of Aβ are the residues of Aβ. It can be co-administered with an antibody specific for an epitope other than groups 3-7. Such antibodies can be administered sequentially or simultaneously. Antibodies against amyloid components other than Aβ can also be used (eg administration or co-administration).

The epitope specificity of an antibody can be identified, for example, by forming a phage display library in which different members represent different subsequences of Aβ. A phage display library is then selected for members that specifically bind to the antibody being tested. Isolate a family of sequences. Typically, such families contain a common core sequence and varying lengths of flanking sequences within different members. The shortest core sequence that exhibits specific binding to the antibody canonicalizes the epitope bound by the antibody. The antibody
Epitope specificity can also be tested in a competitive assay using antibodies whose epitope specificity has already been determined. For example, an antibody that competes with the 12A11 antibody for binding to Aβ is 12
It binds within the same or similar epitope as A11, ie, residues Aβ3-7. Screening antibodies for epitope specificity is a useful predictor of therapeutic efficacy. For example, A
Antibodies determined to bind to an epitope within residues 1-7 of β may be effective for preventing and treating Alzheimer's disease according to the methodology of the present invention.

An antibody that specifically binds to a preferred segment of Aβ without binding to other regions of Aβ is
There are a number of advantages compared to monoclonal antibodies that bind to other regions or polyclonal sera to native Aβ. First, for the same dose weight, the dose of antibody that specifically binds to the preferred segment comprises a higher molar reference dose of antibody that is effective in removing amyloid plaques. Second, an antibody that specifically binds to the preferred segment is the native APP.
It can induce a removal response to amyloid deposits without inducing a removal response to the polypeptide, thereby reducing the expected side effects.
1. Production of non-human antibodies The present invention features non-human antibodies, such as antibodies having specificity for a preferred Aβ epitope of the present invention. Such antibodies can be used in formulating various therapeutic compositions of the invention, or preferably provide complementarity determining regions for the production of humanized or chimeric antibodies (details are described below). . Production of non-human, eg, murine, guinea pig, primate, rabbit or rat, monoclonal antibodies can be accomplished, for example, by immunizing animals with Aβ. Longer polypeptides comprising Aβ or an immunogenic fragment of Aβ or anti-idiotype antibodies to antibodies to Aβ can also be used. Harlow & La
ne, see the above document (incorporated by reference in its entirety for all purposes). Such immunogens can be obtained from the original source, by peptide synthesis or by recombinant expression. Optionally, the immunogen can be administered in a fusion or otherwise complex with the carrier protein, as described below. Optionally, the immunogen can be administered with an adjuvant. The term “adjuvant” refers to a compound that enhances an immune response to an antigen when administered with an antigen, but does not elicit an immune response to the antigen when administered alone. Adjuvant is lymphocyte recruitment (recruitmen)
t) The immune response can be enhanced by various mechanisms including stimulation of B and / or T cells and stimulation of macrophages. Various types of adjuvants are used as described below. Complete Freund's adjuvant followed by incomplete adjuvant is preferred for immunization of laboratory animals.

Rabbits or guinea pigs are typically used to produce polyclonal antibodies. For example, an exemplary production of a polyclonal antibody for passive protection can be performed as follows. 125 non-transgenic mice are immunized with 100 μl of Aβ 1-42 plus CFA / IFA adjuvant and euthanized 4-5 months later. Blood is collected from the immunized mouse. IgG is separated from blood components. Antibodies specific for the immunogen may be partially purified by affinity chromatography. An average of about 0.5-1 mg of immunogen-specific antibody is obtained per mouse, giving a total of 60-120 mg.

A mouse is typically used to produce a monoclonal antibody. A in the mouse
A monoclonal antibody to the fragment can be produced by injecting a fragment or longer form of β, producing a hybridoma, and screening the hybridoma for antibodies that specifically bind to Aβ. Optionally, antibodies are screened for binding to a particular region of Aβ or a desired fragment without binding to other non-overlapping fragments of Aβ. The latter screen can be accomplished by determining the binding of the antibody to a collection of deletion mutants of Aβ peptide and determining which deletion mutant has bound to the antibody. Binding can be assessed, for example, by Western blot or ELISA. The smallest fragment that exhibits specific binding to the antibody canonicalizes the epitope of the antibody. Or
Epitope specificity can be determined by competition assays in which the test and reference antibody compete for binding to Aβ. When the test and reference antibodies compete, they bind to an epitope that is sufficiently proximal such that binding of the same epitope or one antibody interferes with other binding.
Preferred isotypes for such antibodies are mouse isotype IgG2a or other species equivalent isotype. Mouse isotype IgG2a is equivalent to human isotype IgG1 (eg, human IgG1).
2. Chimeric and humanized antibodies The invention also features chimeric and / or humanized antibodies specific for beta amyloid peptides (ie, chimeric and / or humanized immunoglobulins). Chimeric and / or humanized antibodies have the same or similar binding specificity and affinity as mouse or other non-human antibodies that provide starting material for the construction of chimeric or humanized antibodies.
a. Production of Chimeric Antibody The term “chimeric antibody” refers to an antibody whose light and heavy chain genes are constructed from immunoglobulin gene segments belonging to different species, typically by genetic engineering. For example, the variable (V) segment of a gene from a mouse monoclonal antibody will bind to human constant (C) segments such as IgG1 and IgG4. Human isotype IgG1
Is preferred. Thus, a typical chimeric antibody is a hybrid protein consisting of a V or antigen binding domain from a murine antibody and a C or effector domain from a human antibody.
b. Production of Humanized Antibody The term “humanized antibody” consists essentially of at least one chain (referred to as an acceptor immunoglobulin or antibody) comprising a variable region framework residue from a human antibody chain and essentially a mouse. Refers to an antibody comprising at least one complementarity determining region (referred to as donor immunoglobulin or antibody) from the antibody. Queen et al. , Proc. Na
tl. Acad. Sci. USA 86: 10029-10033 (1989), US Pat. No. 5,530,101, US Pat. No. 5,585,089, US Pat. No. 5,693,761, US Pat. 693,762, Selick
et al. , International Patent Publication (WO) 90/07861 and Winter US Pat. No. 5,225,539, which are incorporated by reference in their entirety for all purposes. If present, the constant region is also substantially or completely derived from human immunoglobulin.

Replacement of mouse CDRs within the human variable domain framework results in retention of their correct spatial orientation when the human variable domain framework assumes a similar or similar conformation to the murine variable framework from which the CDRs were derived. Most likely. This is accomplished by obtaining a human variable domain from a human antibody in which the CDR-derived murine variable framework domain and the human antibody framework sequence show a high degree of sequence identity. The heavy and light chain variable framework regions can be derived from the same or different human antibody sequences. The human antibody sequence can be the sequence of a naturally occurring human antibody or can be the consensus sequence of several human antibodies. Kettleboro
gh et al. , Protein Engineering 4: 773 (1991
): Kolbinger et al. , Protein Engineering 6
: 971 (1992) and Carter et al. , International Patent Publication (WO) No. 92
Refer to the specification of / 22653.

Once the complementarity-determining regions of the murine donor immunoglobulin and the appropriate human acceptor immunoglobulin are identified, the next step is to determine the residue to which the component must be substituted, if any, and to obtain the resulting humanization To optimize the properties of the antibody. In general, substitution of human amino acid residues with mice should be minimized, as the introduction of murine residues is an antibody that excites the human-anti-mouse-antibody (HAMA) reaction in humans. It increases the risk. Art-recognized methods for determining human responses can be performed by monitoring HANA responses in specific patients or during clinical trials. Patients who have been administered the humanized antibody can undergo an immunogenicity assessment during and during the start of the treatment regimen. HAMA reactions are directed against humanized therapeutic reagents in serum samples from patients using methods known to those skilled in the art including, for example, surface plasmon resonance technology (BIACORE) and / or solid phase ELISA analysis. The antibody is detected and measured.

Some amino acids from the human variable region framework residues are selected for substitution based on their possible impact on CDR conformation and / or binding to antigen. Intrinsic alignment of murine CDR regions with human variable framework regions results in an unusual conformational constraint that results in a loss of binding affinity unless corrected by substitution of some amino acid residues. Lead.

The selection of amino acid residues for substitution is determined in part by computer modeling. Computer hardware and software are described herein for generating three-dimensional images of immunoglobulin molecules. In general, a molecular model is created starting from a solved structure for an immunoglobulin chain or domain thereof. The chains to be model compared are compared for amino acid sequence similarity with the elucidated three-dimensional chain or domain, and the chain or domain that exhibits the greatest sequence identity is used as a starting point for the construction of the molecular model. select. Strands or domains sharing at least 50% sequence identity are selected for modeling and preferably at least 60
Those that share%, 70%, 80%, 90% sequence identity or more are selected for modeling. The elucidated starting structure is altered to allow for differences between the actual amino acids in the immunoglobulin chains or domains being compared to those in the starting structure.
The altered structure is then assembled into a composite immunoglobulin. Finally, the model is improved by energy minimization and confirming that all atoms are within a reasonable distance from each other and that the bond length and angle are within chemically acceptable limits.

The selection of amino acids for substitution can also be determined in part by examining the properties of the amino acid at a particular location, or by experimental observation of the effect of substitution or mutagenesis of a particular amino acid. For example, if an amino acid differs between a murine variable region framework residue and a selected human variable region framework residue, the human framework amino acid is: (1) the antigen directly and non-covalently binds the antigen. Combine or
(2) is adjacent to the CDR region,
(3) interact with the CDR region in other ways (eg within 3-6 cm of the CDR region as determined by computer modeling) or (4) reasonably involved in the VL-VH interface When expected, it should normally be replaced with an equivalent framework amino acid from the murine antibody.

Residues that “directly bind antigens non-covalently” interact directly with amino acids on the antigen by established chemical forces such as hydrogen bonding, van der Waals forces, hydrophobic interactions, etc. Contains amino acids at positions within framework regions that have a high probability.

CDR and framework regions are described in Kabat et al. Or Chothia
et al. (Supra document). Kabat et al. The framework region defined by (supra document) is described in Chothia et al. When constructing structural loop residues as defined by (above references), amino acids present in mouse antibodies may be selected for substitution in humanized antibodies. A residue “adjacent to a CDR region” refers to a position immediately adjacent to one or more of the CDRs in the primary sequence of a humanized immunoglobulin chain, eg, a CDR defined by Kabat or a CDR defined by Chothis. Contains amino acid residues in immediate adjacent positions (eg Chothia and Lesk,
JMB 196: 901 (1987)). Those amino acids are particularly likely to interact with amino acids in the CDRs, and when selected from the acceptor, will deform the donor CDRs and reduce the affinity. In addition, adjacent amino acids may interact directly with the antigen (Amit et al. Science, 233: 747 (1986).
The selection of those amino acids from the donor) would be desirable to maintain all antigen contacts that provide affinity within the original antigen.

Residues that “interact with the CDR region in other ways” include those that are determined by secondary structure analysis to be in sufficient spatial arrangement to affect the CDR region. In one embodiment, residues that “interact with the CDR regions in other ways” are identified by analyzing a three-dimensional model (eg, a computer generated model) of the donor immunoglobulin. Typically, a three-dimensional model of the original donor antibody is highly likely that certain amino acids outside the CDR are close to the CDR and interact with amino acids in the CDR by hydrogen bonding, van der Waals forces, hydrophobic interactions, etc. It shows having sex. At those amino acid positions, the donor immunoglobulin amino acid will be selected over the acceptor immunoglobulin amino acid. Amino acids according to this criterion generally have an atom that has a side chain atom within about 3 Å units from some atom in the CDR and that can interact with the CDR atom according to established chemical forces, such as those listed above. Must be included.

For atoms that can form hydrophobic bonds, 3 結合 is measured between their nuclei, but for atoms that do not form bonds, 3Å is measured between their van der Waals surfaces. Thus, in the latter case, the nuclei must be within about 6 cm (3 cm plus the sum of the van der Waals radii) relative to the atoms considered to be able to interact. In many cases, the nuclei are 4 or 5 or 6 cm apart. When determining whether an amino acid can interact with a CDR, it is preferred not to consider the last eight amino acids of heavy chain CDR2 as part of the CDR, because, from a structural point of view, these eight amino acids are This is because they often act as part of the framework.

Amino acids that can interact with amino acids in the CDRs may be identified in yet another way.
The solvent accessible surface area of each framework amino acid is calculated in two ways: (1) within the original antibody and (2) within the virtual molecule consisting of the antibody from which its CDRs have been removed.
A significant difference between those numbers of about 10 square centimeters or more indicates that the access of framework amino acids to the solvent is at least partially blocked by the CDRs, and thus the amino acids are in contact with the CDRs. The solvent accessible surface area of amino acids may be calculated based on a three-dimensional model of the antibody using algorithms known in the art (eg, Co
nnolly, J.M. Appl. Cryst. 16: 548 (1983) and Lee a
nd Richards, J.A. Mol. Biol. 55: 379 (1971), both of which are incorporated herein by reference). Framework amino acids, on the other hand, will affect the conformation of other framework amino acids that are in contact with the CDRs and possibly also interact indirectly with the CDRs.

Amino acids at several positions within the framework are known to be important for determining CDR confirmation (eg, being able to interact with CDRs) in many antibodies (Cho
thia and Lest, supra, Chothia et al, supra, and Tramontano et al, J. Am. Mol. Biol. 215: 175 (199
0), all incorporated herein by reference). These investigators identified conserved framework residues important for CDR conformation by analyzing the structure of several known antibodies. The analyzed antibodies were classified into a limited number of structural or “canonical” classes based on the CDR conformation. Conserved framework residues within members of the canonical class are referred to as “canonical” residues. The canonical residues are residues 2, 25, 29, 30, 33, 48, 64, 71, 90, 94 and 95 of the light chain.
And heavy chain residues 24, 26, 29, 34, 54, 55, 71 and 94. Another residue (eg, CDR structure-determined residue) is Martin and Thorton (199
6) J. et al. Mol. Biol. 263: 800 methodology. Importantly, amino acids at positions 2, 48, 64 and 71 of the light chain and positions 26-30, 71 and 94 of the heavy chain (numbering according to Kabat) can interact with CDRs in many antibodies. It has been known. Amino acids at positions 35 in the light chain and 93 and 103 in the heavy chain also appear to interact with the CDRs. Other residues that appear to affect CDR conformation are described by Foote and Winter (1992) J. MoI. Mol. Biol. 224:
Can be identified according to 487 methodology. Such residues are referred to as “vernier” residues and C
Residues in the framework regions that are directly below the DR (ie, form the “platform” below). The choice of donor amino acids is preferred over the choice of acceptor amino acids (if present) because they are present in the humanized immunoglobulin at all their numbered positions. Conversely, some residues that can interact with the CDR regions, such as the first five amino acids of the light chain, may be selected from the acceptor immunoglobulin without loss of affinity for the humanized immunoglobulin.

Residues “participating in the VL-VH interface” or “packing residues” are for example Novotny
and Habor, Proc. Natl. Acad. Sci. USA, 82: 4592.
-66 (1985) or Chothia et al. , (The above-mentioned document) includes residues at the interface between VL and VH. In general, if the unusual filling residues are different from those in the human framework, they should be retained in the humanized antibody.

In general, one or more amino acids that meet the above criteria can be substituted. In some embodiments, all or most of the amino acids that meet the above criteria are substituted. Sometimes there is some ambiguity as to whether a particular amino acid meets the above criteria, and another variant immunoglobulin is produced, one with that particular substitution and the other with Not. Another variant immunoglobulin thus produced can be tested for the desired activity in any of the assays described herein and the preferred immunoglobulin selected.

Usually, the CDR regions within a humanized antibody are essentially identical, and more generally are identical to the corresponding CDR regions of the donor antibody. However, in certain embodiments, one or more CDs are used to alter the antigen binding specificity of the antibody and / or reduce the immunogenicity of the antibody.
It may be desirable to change the R region. Typically, one or more residues of the CDRs are altered to alter the binding to provide a more favorable on-rate for binding.
), More favorable off-rate of binding, or both,
An idealized coupling constant is reached. Using this strategy, very high binding affinity, eg 1
Antibodies with 0 ¹⁰ M ⁻¹ or higher can be achieved. In summary, donor CDR sequences are preferred as base sequences in which one or more residues are subsequently modified. The affinity maturation techniques described herein can be used to modify the CDR regions and then screen the resulting binding molecules for the desired change in binding. The method involves donor C
The DR, typically the mouse CDR, may be modified to make it less immunogenic so that possible human anti-mouse antibody (HAMA) responses are minimized or avoided. Therefore,
As the CDRs are modified, changes in binding affinity as well as immunogenicity are monitored and scored so that the antibody is optimized for best combined binding and low immunogenicity (
For example, US Pat. No. 6,656,467 and US Patent Publication US20020164.
326A1).

In another solution, the contribution of each individual CDR to antibody binding and / or immunogenicity by analyzing the CDR regions of the antibody and systematically replacing each of the donor CDRs with a human counterpart. To decide. The resulting panel of humanized antibodies is then scored for antibody affinity and possible immunogenicity of each CDR. In this way, two clinically important properties of the candidate binding molecule are determined: antigen binding and low immunogenicity. If patient sera are available for the corresponding murine or CDR-grafted (humanized) form of the antibody, a full panel of antibodies representing a systematic human CDR exchange will be screened for each donor CD.
Anti-idiotypic responses to R can be determined in patients (for technical details see, for example, Iwashi et al., Mol. Immunol. 36: 1079-91 (199
9)). Such a solution makes it possible to identify essential donor CDR regions from non-essential donor CDRs. The non-essential CDR region may then be replaced with a human counterpart CDR. If the essential CDR region cannot be exchanged without unacceptable loss of function, identification of CDR specificity determining residues (SDRs) is performed, for example, by site-directed mutagenesis.
In this way, the CDRs can be re-engineered to retain only the SDR and be human and / or minimally immunogenic at the remaining amino acid positions throughout the CDRs. Such a solution for grafting only some donor CDRs is also referred to as a shortened CDR grafting method (for technical details of the above technique see, for example, Tamura et al., J. of Immunolo.
gy 164 (3): 1432-41 (2000); Gonzales et al. ,
Mol. Immunol. 40: 337-349 (2003); Kashmiri et
al. , Crit Rev. Oncol. Hematol. 38: 3-16 (2001
); And DePascalis et al. , J .; of Immunology 1
69 (6): 3076-84 (2002)).

Furthermore, it is sometimes possible to make one or more conservative amino acid substitutions of CDR residues without appreciably affecting the binding affinity of the resulting humanized immunoglobulin. Conservative substitutions include gly, ala; val, ile, leu; asp, glu;
Combinations such as asn, gln; ser, thr; lys, arg; and phr, tyr are contemplated.

Another candidate substitution is an acceptor human framework amino acid that is unusual or “rare” for the human immunoglobulin at that position. Those amino acids can be substituted with amino acids from the equivalent position of the mouse donor antibody or from the equivalent position of a typical human immunoglobulin. For example, if an amino acid in the human framework region of the acceptor immunoglobulin is rare for that position and the corresponding amino acid in the donor immunoglobulin is common to that position in the human immunoglobulin sequence; or within the acceptor immunoglobulin Substitution may be desirable when one of the amino acids is rare for that position and the corresponding amino acid in the donor immunoglobulin is also rare relative to other human sequences.
Whether a residue is rare for an acceptor human framework sequence must also be considered when selecting a residue for backmutation based on its contribution to the CDR conformation. For example, if a backmutation results in a substitution of a residue that is rare for an acceptor human framework sequence, the humanized antibody may be tested for activity. If a back mutation is not necessary for activity, it may be removed to reduce immunogenicity. For example, back mutations at the following residues may introduce residues that are rare within the acceptor human framework sequence. uk = v2 (2.0%), L3 (
0.4%), T7 (1.8%), Q18 (0.2%), L83 (1.2%), I85 (2
. 9%), A100 (0.3%) and L106 (1.1%); and vh = T3 (2.
0%), K5 (1.8%), I11 (0.2%), S23 (1.5%), F24 (1.5
%), S41 (2.3%), K71 (2.4%), R75 (1.4%), I82 (1.4
%), D83 (2.2%) and L109 (0.8%). These criteria help to ensure that atypical amino acids within the human framework do not destroy the antibody structure. Furthermore, by replacing an unusual human acceptor amino acid with an amino acid from a donor antibody that happens to be typical of a human antibody, a humanized antibody could be made less immunogenic.

As used herein, the term “rare” refers to no more than about 20%, preferably no more than about 10%, more preferably no more than about 5%, still more preferably no more than about 20% of the sequences in a representative sample of sequences. 3
%, More preferably less than about 2%, and even more preferably less than about 1% amino acids appearing at that position, and as used herein “community”
on) "refers to amino acids that occur in about 25% or more, but usually more than about 50% of the sequences in a representative sample. For example, the amino acids in the human acceptor sequence are “rare” or “
It is often preferred to consider only human variable region sequences when determining whether they are `` common '' and mouse variable regions when determining whether mouse amino acids are `` rare '' or `` common '' It is often preferable to consider only the sequence. In addition, all human light and heavy chain variable region sequences are each grouped into “subgroups” of sequences that are particularly homologous to each other and have the same amino acids at certain critical positions (Kabal et al.
al. , Above literature). Often, only those human sequences within the same subgroup as the acceptor sequence are considered to determine whether the amino acids in the human acceptor sequence are “rare” or “common” between the human sequences. preferable.

Another candidate for substitution is an acceptor human framework amino acid that would be identified as part of a CDR region according to another definition proposed in Chothia et al (supra). Additional candidates for substitution are acceptor human framework amino acids that will be identified as part of the CDR regions by AbM and / or contact definition.

Additional candidates for substitution are acceptor framework residues that correspond to rare or unusual donor framework residues. A rare or unusual donor framework residue is one that is rare or unusual (defined herein) for a murine antibody at that position. For murine antibodies, subgroups can be determined according to Kabat and are residue positions identified as different from common. Differences in their donor specificity will indicate somatic mutations in the murine sequence that enhance activity. Unusual residues predicted to affect binding (eg, filled canonical and / or vernier residues) are conserved, while residues predicted to be unimportant for binding can be substituted. Rare residues within the 12A11 UK sequence contain I85 (3.6%). Rare residues within the 12A11vh sequence are T3 (1.0%), I11 (1.7%), L12
(1.7%), S41 (2.8%), D83 (1.8%) and A85 (1.8%).

Additional candidates for substitution are non-germline residues present within the acceptor framework region. For example, when an acceptor antibody chain (ie, a human antibody chain that shares significant sequence identity with a donor antibody chain) is aligned with a germline antibody chain (also shares significant sequence identity with a donor chain), Residues that do not match between the acceptor chain framework and the germline sequence framework can be replaced with corresponding residues from the germline sequence.

In addition to the specific amino acid substitutions discussed above, the framework regions of humanized immunoglobulins are usually essentially identical, and more usually the identity of the framework regions of the human antibody from which they were derived. To do. Of course, a large number of amino acids within the framework region contribute little or no direct to the specificity or affinity of the antibody. Thus, a large number of individual conservative substitutions of framework residues are acceptable with no appreciable change in the specificity or affinity of the resulting humanized immunoglobulin. Thus, in one embodiment, the variable framework region of a humanized immunoglobulin shares at least 85% sequence identity with the human variable framework region sequence or the consensus of such a sequence. In another aspect, the variable framework region of a humanized immunoglobulin is at least 90%, preferably 95%, more preferably 96%, 97 with a human variable framework region sequence or consensus of such a sequence.
Shares%, 98% or 99% sequence identity. However, in general such substitution is not desirable.

In exemplary embodiments, the humanized antibodies of the invention exhibit a specific binding affinity for an antigen of at least 10 ⁷ , 10 ⁸ , 10 ⁹ or 10 ¹⁰ M ⁻¹ . In another aspect, an antibody of the invention can have a binding affinity of at least 10 ¹⁰ , 10 ¹¹ or 10 ¹² M ⁻¹ . Usually, the upper limit of the binding affinity of a humanized antibody for an antigen is within a factor of 3, 4 or 5 of that of the donor immunoglobulin. Often the lower limit of binding affinity is also within a factor of 3, 4 or 5 of that of the donor immunoglobulin. Alternatively, the binding affinity is a humanized antibody without substitution (eg, an antibody with donor CDR and acceptor FR but no FR substitution).
Can be compared with In such cases, the binding of the optimized antibody (with substitution) is preferably at least 2-3 times greater than that of the unsubstituted antibody, or 3-4 times greater. For comparison purposes, the activity of various antibodies can be determined, for example, by BIACORE (ie, surface plasmon resonance using unlabeled reagents) or similar binding assays.
c. Production of Humanized 12A11 Antibodies Preferred embodiments of the invention feature humanized antibodies to the N-terminus of Aβ, particularly for use in the therapeutic and / or diagnostic methodologies described herein. A preferred starting material for the production of humanized antibodies is monoclonal antibody 12A11. 12A11 is specific for the N-terminus of Aβ and (1) has high binding activity for aggregated Aβ1-42, (2) has the ability to capture soluble Aβ, and (3) amyloid It has been demonstrated to mediate plaque phagocytosis (eg, phagocytosis induction) (see Example I). The in vivo efficacy of the 12A11 antibody is described in Example II. Cloning and sequencing of the cDNA encoding the 12A11 antibody heavy and light chain variable regions is described in Example III.

Suitable human acceptor antibody sequences can be identified by computer comparison of the amino acid sequence of the mouse variable region with the sequence of known human antibodies. The comparison is done separately for heavy and light chains, but the principle is the same for each. Specifically, variable domains from human antibodies whose framework sequences exhibit a high degree of sequence identity with the murine VL and VH framework regions are, for example, Kabat database or NCBI IgG for each murine framework sequence. It is identified by searching the IgG protein sequence database using BLAST (published and accessible via the National Institute of Health NCBI Internet server). In one embodiment, an acceptor sequence is selected that shares 50% or more sequence identity with a murine donor sequence, such as a donor framework (FR) sequence. Preferably, 60%, 65%, 70%,
Acceptor antibody sequences that share 75%, 80%, 85%, 90%, 95% sequence identity or more are selected.

A computer comparison of 12A11 shows that the 12A11 light chain (mouse subgroup II)
Reveals the greatest sequence identity with the human light chain of subtype kappa II and that the 12A11 heavy chain (mouse subgroup Ib) exhibits the greatest sequence identity to the human heavy chain of subtype II Kabat et al. Defined by the above references. Light and heavy chain human framework regions can be derived from human antibodies of their subtypes or from consensus sequences of such subtypes. In initial humanization efforts, the light chain variable framework region was derived from human subgroup II antibodies. Based on previous experiments designed to reach high levels of expression of humanized antibodies with heavy chain variable framework regions derived from human subgroup II antibodies, the level of expression of such antibodies is sometimes low It's been found. Thus, Saldanha et al. (1999) Mol. Im
munol. 36: 709-719, framework regions from human subgroup III antibodies were selected instead of human subgroup II.

Human subgroup II antibody K64 (ALMS4) (accession number BAC0173)
3) was identified from the NCBI non-redundant database with significant sequence identity within the light chain variable region of 12A11. Human subgroup III antibody M72 (accession number AAA69
734) was identified from the NCBI non-redundant database with significant sequence identity within the heavy chain variable region of 12A11 (Schroeder and Wang (1990) Proc.
Natl. Acad. Sci. USA 872: 6146-6150).

  Another light chain acceptor sequence includes, for example:

  Alternative heavy chain acceptor sequences include, for example:

In an exemplary embodiment, the humanized antibody of the invention comprises 12 from the acceptor sequence displayed above.
Includes A11 CDR and FR. Residues within the framework regions important for CDR conformation and / or activity described herein are selected for backmutation (if different between donor and acceptor sequences).

Residues are then selected for substitution as follows. When the amino acid differs between the 12A11 variable framework region and the equivalent human variable framework region, the amino acid is
(1) The antigen is directly bound non-covalently,
(2) Adjacent to the CDR region or Chothia et al. Is part of a CDR region in another definition proposed in (above) or interacts with the CDR region in another way (eg within about 3 cm of the CDR region), or (3) If it is reasonably expected to be involved in the VL-VH interface, human framework amino acids should usually be replaced by equivalent mouse amino acids.

Structural analysis of the 12A11 antibody heavy and light chain variable regions, and humanization of the 12A11 antibody are described in Example V. In summary, the murine antibody structure 1 elucidated for the light chain 1
Three-dimensional models of 1JRH and 1ETZ were studied for KTR and heavy chain. CD
Another three-dimensional model that can be studied for the identification of residues important for R confirmation (eg vernier residues) is

And for heavy chains

including.

Three-dimensional structural information regarding the antibodies described herein can be found, for example, in Research Co
lab laboratory for Structural Bioinformatics
'Available publicly from the Proten Data Bank (PDB). PDB is W
old Wide Web freely accessible via the Internet and Berm
an et al. , (2000) Nucleic Acids Research,
28: 235. The elucidated three-dimensional structure study is the CDR within 12A11.
Allows identification of interacting residues. Alternatively, 3D models of 12A11VH and VL chains can be generated using computer modeling software. In summary, a three-dimensional model is created based on the closest solved murine antibody structure for heavy and light chains. For this purpose, 1KTR can be used as a template for 12A11 light chain modeling and 1ETZ and 1JRH can be used as templates for heavy chain modeling. This model can be further improved by a series of energy minimization steps to release adverse atomic contacts and optimize electrostatic and van der Waals interactions. Additional three-dimensional analysis and / or modeling can be performed with their solved rat structures and each one
Based on the similarity between the 2A11 chain, for the light chain 2JEL (2.5 Å) and / or 1 TET (2.3 Å) and for the heavy chain (or any other antibody mentioned above) Is 1
This can be done using GGI (2.8 cm).

A computer model of the structure of 12A11 can further serve as a starting point for predicting the three-dimensional structure of an antibody that includes a 12A11 complementarity determining region substituted within the human framework structure. Additional models can be constructed that represent structures when additional amino acid substitutions are introduced.

In general, one, most or all substitutions of amino acids that meet the above criteria are desirable. Accordingly, humanized antibodies of the invention typically comprise a substitution of a human light chain framework residue with a corresponding 12A11 residue at a selected position of at least 1, 2, 3 or more. Humanized antibodies also typically include substitution of a human heavy chain framework residue with the corresponding 12A11 residue at a selected position of at least 1, 2, 3 or more.

However, in some cases, there is some ambiguity as to whether a particular amino acid meets the above criteria, and alternative variant immunoglobulins have been made, one of which has a particular substitution and the other I don't have anything. Where substitution with murine residues introduces a rare residue in a human immunoglobulin at a particular position, it may be desirable to test the antibody for activity in the presence or absence of the particular substitution. If the activity (eg, binding affinity and / or binding specificity) is approximately the same with or without substitution, an antibody without substitution will be preferred, as described herein. This is because it is expected that the induction of the HAMA process is small.

Another candidate for substitution is an acceptor human framework amino acid that is unusual for a human immunoglobulin at that position. Those amino acids can be substituted with amino acids from the equivalent position of more typical human immunoglobulins. Alternatively, amino acids from equivalent positions in mouse 12A11 can be introduced into human framework regions if such amino acids are typical of human immunoglobulins at equivalent positions.

Another candidate for substitution is a non-germline residue present within the framework region. A computer comparison of 12A11 using known germline sequences can identify germline sequences with the highest degree of sequence identity in the heavy or light chain. The alignment of framework regions and germline sequences reveals which residues may be selected for substitution with the corresponding germline residues. Residues that do not match between the selected light chain acceptor framework and one of those germline sequences can be selected for substitution with the corresponding germline sequence residues.

Rare mouse residues compare the donor VL and / or VH sequence with the sequences of other members of the subgroup (by Kabat) to which the donor VL and / or VH sequence belongs,
And it is identified by identifying the residue position different from common. Differences in their donor specificity will indicate somatic mutations that enhance activity. Abnormal or rare residues close to the binding site can come into contact with the antigen, making it desirable to retain mouse residues. However, if an unusual mouse residue is not important for binding, the use of the corresponding acceptor residue is
Mouse residues are preferred because they appear to create an immunogenic neoepitope within the humanized antibody. If the unusual residue in the donor sequence is actually a common residue in the corresponding acceptor sequence,
The preferred residue is clearly the acceptor residue.

  Table 1A is a summary of the sequence analysis of the 12A11 VH and VL regions.

  Describes germline sequences that can be used in selecting amino acid substitutions.

Three-dimensional structural information for the antibodies described herein can be found, for example, in Research Co
lab laboratory for Structural Bioinformatics
Available from s'Protein Data Bank (PDB). PDB
Is freely accessible via the World Wide Web Internet, and Berman et al. (2000) Nucleic Acids Research
h, p235-242. Germline sequences cited herein are, for example, National Center for Biotechnology Information
from the database of national (Inst.
National Library at site of Health (NIH)
as a department of Medicine (NLM)). NCBI “Ig Gerlin”
The homogeneity search of the “e Gene” database is provided by IgG BLAST ^™ .

In an exemplary embodiment, the humanized antibody of the invention comprises (i) murine 12A11 VL.
A light chain comprising a variable domain comprising a CDR and a human acceptor framework, wherein the framework is 0, 1, 2, 3, substituted with the corresponding 12A11 residue
Has 4, 5, 6, 7, 8, 9 or more residues, and (ii) 12A11 V
A heavy chain comprising an H CDR and a human acceptor framework, wherein the framework is at least 1, 2, 3, 4, 5, 6, 7, substituted with the corresponding 12A11 residue.
It has 8, 9 or more residues and optionally at least 1, preferably 2 or 3, residues substituted by corresponding human germline residues.

In another exemplary embodiment, the humanized antibody of the invention comprises (i) murine 12A11 VL CDRs.
And a light chain comprising a variable domain comprising a human acceptor framework, the framework comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9 or more reversion suddenly Containing mutated residues (ie, substituted with the corresponding 12A11 residue),
Where the backmutation is at a canonical, filling and / or vernier residue and (i
i) A heavy chain comprising a 12A11 VH CDR and a human acceptor framework, the framework comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9 or more backmutations Wherein the backmutation is at a canonical, filling and / or vernier residue. In some embodiments, the backmutation is present only at the filling and / or canonical residue or mainly at the filling and / or canonical residue (eg, one of the vernier residues that differs between the donor and acceptor sequences). Or only two vernier residues are backmutated).

In another aspect, the humanized antibody comprises the least possible number of back mutations and is a donor antibody (
Or a chimeric version thereof) that retains equivalent binding affinity. To arrive at such a version, various combinations of backmutations can be eliminated and the resulting antibodies are tested for potency (eg, binding affinity). For example, back mutations in vernier residues (eg, 1, 2, 3, or 4 back mutations) can be eliminated, or back mutations in vernier and filling, vernier and canonical or filling and canonical combinations are eliminated it can.

In another aspect, the humanized antibody of the invention has structural features as described herein,
And at least one (preferably 2, 3, 4 or all) of the following activities: (1) bind soluble Aβ, (2) bind aggregated Aβ1-42 (eg, ELIS
(3) Capture soluble Aβ. (4) plaques (eg AD and / or
Or bind Aβ within PDAPP plaques), (5) 2-3 more than chimeric 12A11
Binds Aβ with fold lower affinity (eg, 12A11 with murine variable region sequences and human constant region sequences), (6) mediates phagocytosis of Aβ (eg, as described herein, ex vivo (7) in the phagocytosis assay), and (7) cross the blood brain barrier (eg to demonstrate short-term brain localization, eg, in a PDAPP animal model, as described herein).

In another aspect, the humanized antibody of the present invention, as described herein, has the following in vi
Has structural features that bind Aβ in a manner or with sufficient affinity to induce at least one vo effect: (1) reduce Aβ plaque burden, (2) prevent plaque formation, (3) soluble Reduce Aβ levels, (4) reduce neuritic conditions associated with amyloid disorders, (5) reduce or reduce at least one physiological condition associated with amyloid disorders, and / or (6) cognitive function Improve.

In other embodiments, the humanized antibody of the invention specifically binds to a structural feature as described herein, and an epitope comprising residues 3-7 of Aβ.

In yet another aspect, a humanized antibody of the invention binds to an N-terminal epitope within Aβ (eg, binds to an epitope within amino acids 3-7 of Aβ) as described herein. And (1) Aβ peptide level, (2) Aβ plaque burden, and (3
) Reduce the neuritis burden or neuritis dystrophy associated with amyloid disorders.

The activity described above can be determined using any one of the various assays described herein or in the art (eg, binding assays, phagocytosis assays, etc.). Activity can be assessed either in vivo (eg, using labeled assay components and / or imaging techniques) or in vitro (eg, using a sample or test strip derived from the subject). Activity can be assessed either directly or indirectly. In certain preferred embodiments, neurological endpoints (eg, amyloid load, neuritis load, etc.) are assessed. Such endpoints can be assessed in vivo (eg, in an animal model of Alzheimer's disease or in a human patient undergoing immunotherapy, for example) using non-invasive detection methods.
Alternatively, such endpoints can be evaluated in post-mortem subjects. Evaluation of such endpoints in postmortem animal models and / or human patients is useful for evaluating the effectiveness of various agents (eg, humanized antibodies) for using similar immunotherapeutic applications. In another preferred embodiment, behavioral or neurological parameters can be assessed as an indication of the neuropathological activity or endpoint described above.
3. Production of Variable Regions Once the CDRs and framework components of a humanized immunoglobulin are selected conceptually, a variety of methods are available for producing such immunoglobulins. In general, one or more of the murine complementarity determining regions (CDRs) of the heavy and / or light chain of an antibody are combined with one or more human frameworks using, for example, primer-based polymerase chain reaction (PCR). Can be humanized by placing within the region. In summary, primers can be designed that can anneal to a target murine CDR region that also includes sequences that overlap and can be annealed using human framework regions. Thus, under appropriate conditions, primers can amplify murine CDRs from murine antibody template nucleic acids and add portions of human framework sequences to the amplified template. Similarly, primers can be designed such that PCR reactions using those primers can anneal to the target human framework region resulting in an amplified human framework region. The murine CDR regions with overlapping human framework sequences with amplified human framework sequences can then be genetically linked as each amplification product is denatured, annealed, and annealed to other products. Thus, in one or more such reactions, one or more murine CDR regions can be genetically linked to an intervening human framework region.

In certain embodiments, the primer may comprise a desired restriction enzyme recognition sequence, and the resulting PCR amplification sequence may be engineered into a larger gene segment, such as a variable light or heavy chain segment, heavy chain, or vector. Make it easy. In addition, the primers used to amplify either the murine CDR region or the human framework region have the desired mismatch so that different codons are introduced into the murine CDR or human framework region. Also good. A typical non-coincidence introduces modifications within the human framework regions that preserve or improve the structural orientation of the murine CDRs, and thus their binding affinity, as described herein.

It should be understood that the above solution can be used to introduce one, two or all three murine CDRs within the intervening human framework region. Methods for amplifying and linking different sequences using primer-based PCR are described in, for example, Sambrook,
Fritsch and Maniatis, Molecular Cloning: C
old Spring Harbor Laboratory Press (1989)
: DNA Cloning Vol. 1 and 2, (DN Glover Ed.
1985); PCR Handbook Current Protocols in
Nucleic Acid Chemistry, Beaucage, Ed. John
Wiley & Sons (1999) (Editor); Current Proto
cols in Molecular Biology, eds Ausubel et
al. , John Wiley & Sons (1992).

Because of the degeneracy of the code, various nucleic acid sequences encode each immunoglobulin amino acid sequence. The desired nucleic acid sequence can be produced by solid phase DNA synthesis from the beginning or by PCR mutagenesis of previously made variants of the desired polypeptide. Oligonucleotide-mediated mutagenesis is a preferred method for making substitutions, deletions and insertional variants of the target polypeptide DNA. Adelman et al. , DNA 2: 183 (1983). In summary, target polypeptide DNA is modified by hybridizing an oligonucleotide encoding the desired mutation to a single stranded DNA template. Following hybridization, a DNA polymerase is used to incorporate the oligonucleotide plasmid and synthesize the entire second complementary strand of the template encoding the selected modification in the target polypeptide DNA.
4). Selection of Constant Regions The variable segments of antibodies produced as described above (eg, heavy and light chain variable regions of chimeric or humanized antibodies) are replaced by immunoglobulin constant regions (Fc regions), typically human immunoglobulins. It is typically linked to at least a portion of the constant region. Human constant region DNA
The sequence can be isolated from various human cells, but preferably from immortalized B cells according to well-known methods (Kabat et al., Supra and Liu et al., International Patent Publication (WO) 87/02671. (See above) (in each of the above references incorporated by reference in its entirety for all purposes). Ordinarily, the antibody will contain both light and heavy chain constant regions. The heavy chain constant region usually includes CH1, hinge, CH2, CH3 and CH4 regions. The antibodies described herein include antibodies having any type of constant region, including IgM, IgG, IgD, IgA, and IgE, and any isotype, including IgG1, IgG2, IgG3, and IgG4. Where an antibody (eg, a humanized antibody) desires to exhibit cytotoxic activity, the constant region is usually a complement-binding constant domain and the class is typically IgG1. Human isotype IgG1 is preferred. The light chain constant region can be lambda or kappa. A humanized antibody may comprise sequences from one or more classes or isotypes. The antibody may be a tetramer comprising two light chains and two heavy chains, as separate heavy chains, as light chains, as Fab, Fab′F (ab ′) 2 and Fv, or as heavy and light chains. It can be expressed as a single chain antibody in which the variable domains are linked via a spacer.
5. Expression of recombinant antibodies Chimeric and humanized antibodies are typically produced by recombinant expression. Nucleic acids encoding the light and heavy chain variable regions optionally linked to the constant region are inserted into expression vectors. The light and heavy chains can be cloned into the same or different expression vectors. A DNA segment encoding an immunoglobulin chain is operably linked to control sequences in an expression vector that ensures expression of the immunoglobulin polypeptide. Expression control sequences include, but are not limited to, promoters (eg, naturally associated or heterologous promoters), signal sequences, enhancer elements, and transcription termination sequences. Preferably, the expression control sequence is a eukaryotic promoter system in a vector that can transform or transfect eukaryotic host cells (eg, COS cells). Once the vector is integrated into a suitable host cell, the host is kept under conditions suitable for high level expression of the nucleotide sequence and collection and purification of the cross-reacted antibody.

These expression vectors are typically capable of replication in the host organism either as episomes or as an integral part of the host chromosomal DNA. Commonly desired DNA
To allow detection of those cells transformed with the sequence, the expression vector contains a selectable marker (eg, ampicillin resistance, hygromycin resistance, tetracycline resistance, kanamycin resistance or neomycin resistance) (eg, Itakura et al., USA). (See Japanese Patent No. 4,704,362).

E. coli is one prokaryotic host particularly useful for cloning the polynucleotides (eg, DNA sequences) of the present invention. Other microbial hosts suitable for use include Bacillus species such as Bacillus subtilis, and other enterobacteriaceae such as Salmonella, Serratia, and various Pseudomonas species. Expression vectors can also be produced in these prokaryotic hosts, which typically include expression control sequences that are compatible with the host cell (eg, origins of replication), and any number of various well-known promoters. For example, a lactose promoter system, a tryptophan (trp) promoter system, a beta-lactamase promoter system, or a promoter system from phage lambda, which typically regulates expression, optionally with an operator sequence And has ribosome binding site sequences etc. to initiate and complete transcription and translation.

Other microorganisms such as yeast are also useful for expression. Saccharomyces (Saccharomyces)
ce) is a preferred yeast host, while suitable vectors with expression control sequences (eg, promoters, origins of replication, termination sequences, etc. as desired are also useful. Typical promoters include 3-phosphoglycerate Includes kinases and other glycolytic enzymes Inducible yeast promoters include, among others, promoters from alcohol dehydrogenase, isocytochrome C, and enzymes capable of utilizing maltose and galactose.

In addition to microorganisms, mammalian tissue cell cultures may also be used to express and produce the polypeptides of the invention (eg, polynucleotides encoding immunoglobulins or fragments thereof). Winnacker, From Genes to Clones, V
CH Publishers, N.C. Y. , N.M. Y. (1987). Heterologous protein (
Since a number of suitable host cell systems capable of secreting (eg native immunoglobulins) have been developed in the art, eukaryotic cells are actually preferred and CHO cell lines, various C
Including os cell lines, HeLa cells, preferably myeloma cell lines, or transformed B cells or hybridomas. Preferably the cell is non-human. The expression vectors of these cells contain expression control sequences such as origins of replication, promoters, and enhancers (Que
en et al. , Immunol. Rev. 89:49 (1986)), and necessary processing information sites such as ribosome binding sites, RNA splice sites, polyadenylation sites, and transcription terminator sequences. Preferred expression control sequences include immunoglobulin genes, SV40, adenovirus, bovine papilloma virus,
It is a promoter derived from cytomegalovirus. Co. et al. , J
. Immunol. 148: 1149 (1992).

Alternatively, the antibody-encoding sequence can be incorporated into a transgene for introduction into the genome of the transgenic animal and subsequent expression in the milk of the transgenic animal (see, eg, Deboer et al., US Pat. No. 5,741,957, Ro
sen US Pat. No. 5,304,389, and Maede et al. US Pat.
9,992 specification). Suitable transgenes include light and / or heavy chain coding sequences operably linked to promoters and enhancers from mammary gland specific genes, such as casein or beta-lactoglobulin.

Alternatively, an antibody of the present invention (eg, a humanized antibody) is a transgenic plant (tobacco,
Maize, soybean and alfalfa). Improved “plant”
ibody "vector (Hendy et al (1999) J. Immunol. Me
thds 231: 137-146) and purification strategies combined with increases in transformable crop species are not only useful for the treatment of humans and animals but also for industrial applications (eg catalytic antibodies) as well as for recombinant immunoglobulins. A practical and efficient means of production is provided for such methods. Furthermore, plant-produced antibodies have proven to be safe and efficient and avoid the risk of contamination with infectious spongiform encephalopathy (TSE) drugs due to the use of animal-derived materials. Furthermore, differences in glycosylation patterns between plants and mammalian cell-produced antibodies have little or no effect on antigen binding or specificity. In addition, no signs of toxicity or HAMA were observed in patients who received topical oral application of plant-derived secreted dimeric IgA antibodies (Larrick et a
l. (1998) Res. Immunol. 149: 603-608).

Various methods may be used to express recombinant antibodies in transgenic plants. For example, antibody heavy and light chains can be expressed in expression vectors (eg, Agrobacterium t
vectors that can be cloned independently into umefaciens vectors) and then transformed into plant tissues in vitro using recombinant bacteria or physically introduced into plant tissues using, for example, ballistics Can be transformed with, for example, particles. Subsequently, the whole plant expressing the individual chains is reconstructed and then finally sexually crossed by their sexual crossing, resulting in the production of functional antibodies. A similar protocol was used to express functional antibodies in tobacco plants (
Hiatt et al. (1989) Nature 342: 76-87). In various embodiments, the signal sequence is a suitable plant environment (eg, apoplasm).
Or can be used to promote the expression, binding and folding of non-accumulated antibody chains by directing the chains to the aqueous environment of tubers, other specific plant tissues including fruits or seeds (Fied
ler et al. (1995) Bio / Technology 13: 1090-1
093). Plant bioreactors can also be used to increase antibody yields and significantly reduce costs.

Vectors containing the relevant polynucleotide sequences (eg, heavy and light chain coding and expression control sequences) can be transferred into host cells by well-known methods that vary with the type of cellular host. For example, calcium chloride transfection is commonly used for prokaryotic cells,
On the other hand, calcium phosphate treatment, electroporation, lipofection, biolistics or virus-based transfection may be used for other cellular hosts (generally Sambrook et al., Molecular Cloning: A La
borory Manual (Cold Spring Harbor Press)
, 2nd ed. (1989)) (incorporated by reference in its entirety for all purposes). Other methods used to transform mammalian cells include the use of polybrene, protoplast fusion, liposomes, electroporation, and microinjection (see generally Sambrook, supra). For the generation of transfected animals, the transgene can be microinjected into a fertilized egg, or can be integrated into the genome of an embryonic stem cell, and the nucleus of such a cell can be transferred into an enucleated egg cell.

When the heavy chain and the transformation are cloned on separate expression vectors, the vector is co-transfected to obtain the original immunoglobulin expression and construction. When expressed, all antibodies of the invention, their dimers, individual light and heavy chains, or other immunoglobulin forms can be ammonium sulfate precipitated, affinity columns, column chromatography, HPLC purification, gel electrophoresis, etc. (See, generally, Scopes, Protein Purification (Spr
inger-Verlag, N .; Y. , (1982)). For pharmaceutical use, essentially pure immunoglobulins of at least about 90-95% homogeneity are preferred and 9
A homogeneity of 8 to 99% or higher is most preferred.
6). Antibody fragments Further contemplated within the scope of the present invention are antibody fragments. In one embodiment, non-human and / or chimeric antibody fragments are provided. In another aspect, fragments of humanized antibodies are provided. Typically, the fragments exhibit specific binding to the antigen with an affinity of at least 10 ⁷ , and more typically 10 ⁸ to 10 ⁹ M ⁻¹ . Humanized antibody fragments can be isolated from heavy, light, Fab, Fab ′, F (ab
') Includes 2, Fabc, and Fv. Fragments are produced by recombinant DNA technology or by enzymatic or chemical separation of the native immunoglobulin.
7). Epitope mapping Epitope mapping can be performed to determine which antigenic determinants or epitopes of Aβ are recognized by an antibody. In one embodiment, epitope mapping is performed according to a Replacement NET (rNET) analysis. rNET
Epitope map assays provide information about the contribution of individual residues within an epitope to the overall binding activity of an antibody. rNET analysis uses synthesized systematic single substituted peptide analogs. The binding of the antibody to be tested is determined against the original peptide (the original antigen) and against 19 alternative “single substituted” peptides, each of which is at the first position and at that position. In contrast, it is substituted with one of the 19 unnatural amino acids. A profile is created that reflects the effect of substitution with various non-native residues at that position. A profile is similarly generated at successive positions along the antigenic peptide. In so doing, the combined profile, ie, the epitope map (reflecting substitution of all 19 non-native residues at each position) is compared to the map similarly generated for the second antibody. it can. Essentially similar or identical maps indicate that the antibodies being compared have the same or similar epitope specificity.
8). Testing antibodies for therapeutic efficacy in animal models Each group of 7-9 month old PDAPP mice is injected with 0.5 mg of PBS in polyclonal or specific anti-Aβ monoclonal antibody. The whole antibody preparation is purified until it has a low endotoxin level. A monoclonal antibody injects a fragment or longer form of Aβ into the mouse, produces a hybridoma and specifically binds to the desired fragment of Aβ without binding to other non-overlapping fragments of Aβ. It can be prepared by screening hybridomas for antibodies.

Mice are injected intraperitoneally for 4 months as needed to maintain circulating antibody concentrations as measured by ELISA greater than 1/1000 as defined by ELISA against Aβ42 or other immunogens . Titers are monitored and mice are euthanized at the end of 6 months after injection. Histochemically, Aβ levels and toxicity tests are performed postmortem. Ten mice were used per group.
9. Screening antibodies for scavenging activity The present invention also provides methods for screening for antigens that are desired to remove amyloid deposits or other antigens, or related biological entities that are desired to have scavenging activity. To do. To screen for activity against amyloid deposition, a tissue sample from the brain of a patient with Alzheimer's disease, or an animal model with a characteristic Alzheimer's disease pathology, phagocytic cells containing Fc receptors, such as microglia, and An antibody to be tested;
Contact in the medium in vitro. Phagocytic cells can be primary cultures, or cell lines, and can be murine (eg, BV-2 or C8-B4 cells) or human derived (eg, THP-1 cells). Some methods combine components on a microscope slide to facilitate microscopic monitoring. In some methods, multiple reactions are run in parallel in the wells of a microtiter dish. In such a format, separate miniature microscope slides can be mounted in separate wells or in a non-microscopic detection format, such as Aβ EL
ISA detection can be used. A series of measurements are taken of the amount of amyloid deposits in the in vitro reaction mixture before the reaction proceeds and one or more test values during the reaction. Antigens can be detected by staining with, for example, fluorescently labeled antibodies against Aβ or other amyloid plaque components. The antibody used for staining may or may not be the same as the antibody being tested for scavenging activity. The decrease from baseline in amyloid deposition during the reaction is
Indicates that the antibody being tested has scavenging activity. Such antibodies may be useful for preventing or treating Alzheimer's disease and other amyloid diseases. Antibodies particularly useful for preventing or treating Alzheimer's disease or other amyloid diseases are:
Including those capable of removing both concentrated and diffuse amyloid plaques, eg, 12A of the present invention
11 antibody, or a chimeric or humanized version thereof.

Similar methods can also be used to screen antibodies for activity in removing other types of biological objects. The assay can be used to detect removal activity against virtually any type of biological object. Typically, biological objects have some role in human or animal disease. The biological object can be provided in a tissue sample or in isolated form. When provided as a tissue sample, it is preferably not fixed so that it can be in direct proximity to the components of the tissue sample and to avoid perturbing the conformation of the tissue associated with fixation. Examples of tissue samples that can be tested in this assay include cancerous tissue, precancerous tissue, tissue containing benign growths such as warts or moles, tissue infected with pathogenic microorganisms, tissue infiltrated with inflammatory cells, and intercellular Tissue with pathological stroma (eg, fibrotic pericarditis), tissue with abnormal antigens, and scar tissue. Isolated biological objects that can be used include Aβ, viral antigens or viruses, proteoglycans, antigens of other pathogenic microorganisms, tumor antigens and cell adhesion molecules. Such antigens can be obtained from natural sources, recombinant expression or chemical synthesis, among other means. The tissue sample or isolated biological object is F
A phagocytic cell bearing the c receptor, such as monocytes or microglia, and the antibody to be tested are contacted in the medium. The antibody can be designated a biological object to be tested or an antigen associated with the object. In the latter situation, the purpose is to test whether the biological object phagocytoses with the antigen. Usually, but not necessarily, antibodies and biological objects (sometimes with associated antigens) are brought into contact with each other prior to phagocytic cell addition. Then, if present, the concentration of biological matter and / or associated antigen remaining in the medium is monitored. A decrease in the amount or concentration of the antigen or related biological object in the medium indicates that the antigen has a clearance response to the antigen and / or related biological object together with the phagocytic cells.
10. It may be desirable to alter the effector function of a molecule for the above antibodies of the invention comprising a chimeric / humanized antibody constant region (Fc region) with altered effector function. In general, antibody effector functions reside in the constant or Fc region of various effector molecules, eg, molecules capable of mediating binding to complement proteins or Fc receptors. Complement binding to the Fc region is important, for example, for opsonization and lysis of cellular pathogens and activation of inflammatory responses. For example, the binding of antibodies to Fc receptors on the surface of effector cells can involve a number of important and diverse biological reactions, such as the entrapment and degradation of antibody-coated pathogens or particles, removal of immune complexes, antibody coating Including lysis of the targeted cells by killer cells (ie, antibody-dependent cell-mediated cytotoxicity, or ADCC), release of inflammatory mediators, intra-placental migration of antibodies, and control of immunoglobulin production.

Thus, depending on the particular therapeutic or diagnostic application, only the above immune functions or selected immune functions may be desirable. Various aspects of the effector function of the molecule are achieved, including modifying the Fc region of the antibody to enhance or suppress various reactions of the immune system that have beneficial effects in diagnosis and therapy.

Antibodies of the invention that react only with certain types of Fc receptors can be produced, for example, antibodies of the invention can be produced by deletion or modification of Fc receptor binding sites located within the Fc region of the antibody. Can be modified to bind only to the Fc receptor or to delete the Fc receptor. Other desirable modifications of the Fc region of the antibodies of the invention are listed below. Typically, the Kabat numbering system is used to achieve the desired change in effector function (eg, I
Used to indicate which amino acid residues within the Fc region (of a gG antibody) have been modified (eg, by amino acid substitution). This numbering system is also used to compare antibodies between species so that, for example, the desired effector functions observed in mouse antibodies can be systematically manipulated in the human, humanized, or chimeric antibodies of the invention. Used.

For example, if an antibody (eg, an IgG antibody) is an Fc receptor (eg, an Fc receptor on human monocytes, F
It was observed that groups could be grouped into those found to show tight, intermediate or weak binding to (cγRI). A comparison of amino acid sequences within these different affinity groups identified a monocyte binding site within the hinge junction region (Leu234-Ser239).
Furthermore, human FcγRI receptor has human IgG1 and mouse IgG2a as monomers.
However, the binding of mouse IgG2b is 100 times weaker. A comparison of the sequences of those proteins within the hinge junction region shows that sequences 234-238 within the strong conjugate, ie Leu
-Leu-Gly-Gly-Pro (SEQ ID NO: 32) was shown to be Leu-Glu-Gly-Gly-Pro (SEQ ID NO: 33) in mouse gamma 2b, a weak conjugate. Thus, if low FcγI receptor binding is desired, corresponding changes in the human antibody hinge sequence can be made. It is understood that other modifications can be made to achieve the same or similar results. For example, the affinity of an FcγRI binding can be determined by substituting a designated residue with a residue having an inappropriate functional group on its side chain, or a charged functional group (eg, Glu
Or Asp) or, for example, an aromatic nonpolar group (eg, Phe, Tyr, or Trp)
Can be modified by introducing.

These changes are equally applicable to murine, human and rat systems given the sequence homology between different immunoglobulins. For human IgG3 binding to the human FcγRI receptor, a change from Leu235 to Glu was shown to disrupt the mutant interaction to the receptor. The binding site for this receptor can be inserted or removed by appropriate mutations.

Mutations on or adjacent to the hinge junction region (eg, residues 234, 23 due to ala
6 or 237) at least residues 234,23 in the affinity for the FcγRI receptor
It is shown that modifications within 5, 236 or 237 at least affect. Thus, an antibody of the invention can also have a modified Fc region that has a modified binding affinity for FcγRI compared to an unmodified antibody. Such an antibody conveniently comprises amino acid residues 234, 2
Has a mutation at 35, 236, or 237.

Affinity for Fc receptors can be altered by similar methods by controlling immune responses in different ways.

  As a further example, the solubility of IgG antibodies following binding of the C1 component of complement can be altered.

C1, the first component of the complement system, is C1q, C1r and C1s, which bind tightly together
It comprises three proteins known as C1q has been shown to be involved in the binding of three protein complexes to antibodies.

Thus, the C1q binding activity of an antibody is determined by the heavy chain amino acid residues 318, 320, and 322.
Antibodies can be provided with modified CH2 domains in which at least one of the is changed to a residue with a different side chain. The numbering of the residues in the heavy chain is that of the EU index (see Kabat et al., Supra). Other suitable modifications to modify, eg reduce or eliminate, specific C1q binding to the antibody include residues 318 (Glu
), 320 (Lys), and 322 (Lys) to any one Ala.

Furthermore, as long as residues are mutated at those residues and residue 318 has a hydrogen-bonding side chain and both residues 320 and 322 have a positively charged side chain, the C1q bond is conserved. It has been shown.

C1q binding activity can be abolished by substituting with residues having inappropriate functionality on any one side chain of three specific residues. It is not always necessary to replace the ionic residue only with Ala in order to eliminate the C1q bond. Instead of any one of the three residues to annihilate the C1q bond, other alkyl-substituted nonionic residues such as Gly, Ile, Le
u, or Val, or aromatic nonpolar residues such as Phe, Tyr, Trp and P
It is also possible to use ro. In addition, in order to abolish C1q binding activity, 318
Instead of residues 320 and 322 except for polar nonionic residues such as Ser, Thr
It is also possible to use Cys and Met.

It has also been pointed out that side chains on ionic or nonionic polar residues can form hydrogen bonds in a manner similar to the bond formed by Glu residues. Thus, substitution of a 318 (Glu) residue with a polar residue will modify but not extinguish C1q binding activity.

Replacement of residue 297 (Asn) with Ala results in loss of lytic activity but C1
It is also known that the affinity for q is only slightly reduced (down to about 1/3). This modification destroys the presence of glycosylation sites and carbohydrates necessary for complement activation.
Any other substitution at this site will destroy the glycosylation site.

The present invention also provides an antibody having a modified effector function, wherein the antibody has a modified hinge region. The modified hinge region may comprise a complete hinge region derived from an antibody class or subclass of antibody different from that from the CH1 domain.
For example, the constant region (CH1) of a class IgG antibody can be attached to the hinge region of a class IgG4 antibody. Alternatively, the new hinge region may comprise a portion of the original hinge or repeat unit in which each unit within the repeat is derived from the original hinge region. In one example, a natural hinge region is modified by converting one or more cysteine residues to neutral residues, such as alanine, or converting appropriately positioned residues to cysteine residues. Is done. Such modifications are accomplished using art-recognized protein chemistry, preferably genetic recombination techniques, as described herein.

In one embodiment of the invention, the number of cysteine residues in the hinge region of the antibody is reduced to, for example, one cysteine residue. This modification has the advantage of facilitating the construction of antibodies, eg, antibody molecules in which the bispecific antibody molecule and the Fc moiety are replaced by an effector or reporter molecule, since it forms a single disulfide bond. Because it is necessary only for that. This denaturation also provides a specific target for attachment to the hinge region, either directly or indirectly, eg, by chemical means, to other hinge regions or to either effector or reporter molecules.

Conversely, the number of cysteine residues in the hinge region of the antibody is also increased by, for example, at least one from the number of normally present cysteine residues. Increasing the number of cysteine residues can be used to stabilize the interaction between adjacent hinges. Another benefit of this modification is to facilitate the use of cysteine thiol groups, for example, to attach effector or reporter molecules to radiolabeled modified antibodies.

Thus, the present invention provides for the exchange of hinge regions between antibody classes, particularly IgG classes, and / or increasing or decreasing the number of cysteine residues in the hinge region to achieve altered effector function (See, eg, US Pat. No. 5,677,425, which is expressly incorporated herein). The determination of altered antibody effector function is performed using assays according to other techniques described herein or recognized in the art.

Importantly, the resulting antibody can be subjected to one or more assays to assess any change in biological activity compared to the starting antibody. For example, the ability of an antibody to have an Fc region modified to bind complement or Fc receptors is
The assay disclosed in the present invention can be evaluated using any assay recognized in the art.

Production of the antibodies of the invention is accomplished by any suitable technique, including techniques described herein or techniques known to those skilled in the art. For example, an appropriate protein sequence, such as the relevant constant domain, eg, the Fc region of an antibody, ie, CH2, and / or CH
The three-domain or all of the forming portions thereof can be further synthesized, including appropriate modified residues, and then chemically linked to the appropriate position within the antibody molecule.

Preferably, genetic engineering techniques are used to produce modified antibodies. Preferred techniques include polymerases such that, for example, a DNA sequence encoding at least a portion of an IgG heavy chain, eg, Fc or constant region (eg, CH2, and / or CH3) is modified with at least one or more residues. Preparing primers suitable for use in chain reaction (PCR). The segments can then be operably linked to the remaining portion of the antibody, eg, the variable region of the antibody and regulatory elements for intracellular expression.

The present invention relates to a vector used to transform a cell line, a vector used to produce a transformation vector, a cell transformed with a transforming vector, and a vector transformed with a preparation vector. Also included are cell lines to be converted and methods for their production.

Preferably, the cell line transformed to produce an antibody having a modified Fc region (ie, of altered effector function) is an immortalized mammalian cell line (eg, C
HO cells).

The cell line used for the production of antibodies having a modified Fc region is preferably a mammalian cell line, but any other suitable cell line such as a bacterial cell line or yeast cell line may be used instead. Good.
11. Affinity Mutation Antibodies (eg, humanized antibodies) of the invention can be modified for improved function using any number of affinity mutation techniques. Typically, candidate molecules having binding affinity for a given target molecule are identified and then refined or “matured” using mutagenesis techniques to produce one or more of the desired binding interactions with the target molecule or Bring more relevant candidates. Typically, it is the affinity of the antibody (or binding activity, ie, the combined affinity of the antibody to the target antigen) that is denatured, but other properties of the molecule such as stability, effector function, removal Sex, secretory or transport functions may also be modified separately or in parallel with affinity using affinity mutation techniques.

In an exemplary embodiment, the affinity of an antibody (eg, a humanized antibody of the invention) is increased. For example, an antibody having a binding affinity of at least 10 ⁷ M ⁻¹ , 10 ⁸ M ⁻¹ or 10 ⁹ M ⁻¹ has an affinity of at least 10 ⁹ M ⁻¹ , 10 ¹⁰ M ⁻¹ or 10 ¹¹ M Can mature to be ^-1 .

One solution to the affinity of maturing a binding molecule is to synthesize an amino acid encoding a binding molecule, or portion thereof, that encodes the desired change or changes. Oligonucleotide synthesis is well known in the art and has any desired codon changes.
It is easily automated to produce even species or more nucleic acids. Restriction sites, silent mutations, and preferred codon usage may be introduced in this manner. Alternatively, one or more codons can be modified to represent a specific subset of amino acids, such as a subset that excludes cysteines that can form disulfide linkages, and are limited to canonical regions, such as CDR regions or portions thereof. . Alternatively, the region may be represented by a partially or completely random set of amino acids (for additional details see, for example, US Pat. No. 5,830).
650, US Pat. No. 5,798,208, US Pat. No. 5,824,514, US Pat. No. 5,817,483, US Pat. No. 5,814,476, US Pat. No. 5,723.
323, U.S. Pat. No. 4,528,266, U.S. Pat. No. 4,359,53, U.S. Pat. No. 5,840,479, and U.S. Pat. No. 5,869,644. .

The above solution can be partially or fully performed using polymerase chain reaction (PCR), which methods are well known in the art and include, for example, oligonucleotides with the desired modifications, such as primers or single strands. It is understood that it has a large amount of advantages suitable for incorporating strand nucleic acids and for other manipulations, such as suitable expression or genetic manipulation into cloning vectors. Such PCR can be performed under conditions that allow for differences in nucleotide incorporation and introduce additional changes in the nucleic acid that is amplified thereby. Experimental details and related kits, reagents and primer designs for performing PCR are described, for example, in US Pat. No. 4,683,202, US Pat. No. 4,683,195, US Pat. No. 6,040.
166, and US Pat. No. 6,096,551. Methods for introducing CDR regions into antibody framework regions using primer-based PCR include:
For example, it is described in US Pat. No. 5,858,725. PCR amplification based on primers of an antibody library (and a library generated according to the method) using a minimal set of primers that can find sequence homology using a larger set of antibody molecules, eg, a larger and more diverse set of antibody molecules For example, US Pat. No. 5,780.
225, U.S. Pat. No. 6,303,313, and U.S. Pat. No. 6,479,243. “Kunkel” mutations using non-PCR-based methods for performing site-directed mutagenesis and using single-stranded uracil-containing templates and primers that can introduce hybridization and mutations as they pass through specific E. coli strains Including induction methods (see, eg, US Pat. No. 4,873,192).

Another method of altering antibody sequences or portions thereof is non-optimal (ie error-prone)
Nucleic acid synthesis or PCR of nucleic acids under conditions, denaturation and reconstitution (annealing) of such nucleic acids
Ligation or PCR following exonuclease and / or endonuclease digestion
Reconstruction (nucleic acid shuffling) by, for example, US Pat. No. 6,440,668,
US Pat. No. 6,238,884, US Pat. No. 6,171,820, US Pat.
65,408, U.S. Patent 6,361,974, U.S. Patent 6,358,709,
US Pat. No. 6,352,842, US Pat. No. 4,888,286, US Pat. No. 6,3
37,186, U.S. Patent No. 6,165,793, U.S. Patent No. 6,132,970,
US Pat. No. 6,117,679, US Pat. No. 5,830,721, and US Pat. No. 5,605,793, including one or more combinations of the prior art.

In some embodiments, for example, one or more CDR regions (or portions thereof), one or more framework regions, and / or one or more constant regions (
An antibody library (or affinity maturation library) comprising a family of candidate antibody molecules having diversity within a certain portion of candidate antibody molecules (e.g., constant regions with effector functions) is expressed and the art Can be screened for desired properties using techniques recognized in US Pat. No. 6,291,161, US Pat.
No. 291,160, US Pat. No. 6,291,159, and US Pat. No. 6,291,1
(See each specification of 58). For example, an antibody variable region expression library having CDR3 sequence diversity and a human antibody library having CDR3 sequence diversity by introducing CDR3 sequence diversity by mutagenesis and recovering the library A method of making can be constructed (see, eg, US Pat. No. 6,248,516).

Finally, in order to express affinity matured antibodies, the nucleic acid encoding the candidate antibody molecule can be expressed in appropriate expression formats, such as full-length antibody heavy and light chains (eg, IgG), antibody Fab fragments (
For example, Fab, F (ab ′) ₂ ), or single chain antibodies (scFv) can be introduced into cells using standard vectors and cell transfection / transformation techniques (eg,
US Pat. No. 6,331,415, US Pat. No. 6,103,889, US Pat. No. 5,2
No. 60,203, US Pat. No. 5,258,498, and US Pat. No. 4,946,77
(See each specification of No. 8).
B. An immune response against nucleic acid amyloid deposits encoding immunological and therapeutic agents can also be introduced by the administration of antibodies used for passive immunization and nucleic acids encoding their component chains. Such a nucleic acid may be DNA or RNA. The nucleic acid segment encoding the immunogen is typically linked to a regulatory element that allows expression of the DNA segment in the intended target cell of the patient, such as a promoter or enhancer. For expression in blood cells, which is desirable for induction of an immune response, exemplary promoter and enhancer elements are those from light or heavy chain immunoglobulin genes and / or CMV major intermediate early promoters and enhancers. (Stinski, US Pat. No. 5,168,062, and US Pat. No. 5,385,8).
Each specification of No. 39). Linked regulatory elements and coding sequences are often cloned into vectors. For administration of double chain antibodies, the two chains can be cloned into the same or different vectors.

Retroviral systems (eg Lawrie and Tumin, Cur. Opin. G
enet. Develop. 3: 102-109 (1993)); adenoviral vectors (eg, Bett et al., J. Virol. 67: 5911 (1993)).
See adeno-associated viral vectors (eg Zhou et al., J. Exp. M).
ed. 179: 1867 (1994)); viral vectors from the pox family, including cowpox virus and avian pox virus, viral vectors from the genus alphavirus, such as those derived from Sindbis and Semliki Forest viruses (eg Dubensky)
et al. , J .; Virol. 70: 508 (1996)); Venezuelan equine encephalitis virus (Johnson et al., See US Pat. No. 5,643,576) and rhabdoviruses such as vesicular stomatitis virus (Rose, US Pat. No. 6,1).
68,943) and papilloma virus (Ohe et al., Human)
Gene Therapy 6: 325 (1995); Woo et al. , International Patent Application (WO) 94/12629 and Xiao & Brandsma, N
ucleic Acids. Res. 24: 2630-2622 (1966))). Many viral vector systems are available.

The DNA encoding the immunogen, or a vector containing it, can be packaged in liposomes. Suitable lipids and related analogs are described in Epstein et al. U.S. Pat. No. 5,208,036, Felinger et al. US Patent No. 5
, 264,618, Rose, US Pat. No. 5,279,833, and Epand et al. U.S. Pat. No. 5,283,185.
Vectors and DNA encoding the immunogen can also be adsorbed to or associated with a particulate carrier, examples of which include polymethyl methacrylate polymer and polylactide and poly (lactide-co-glycolide), eg, McGee et al. , J
. Micro Encap. (1996).

Gene therapy vectors or naked polypeptides (eg, DNA) are administered to individual patients, typically systemically (eg, intravenous, intraperitoneal, nasal, gastrointestinal, intradermal, intramuscular, subcutaneous, or intracranial Delivery) or in vivo by topical application (eg Anderso)
n et al. U.S. Pat. No. 5,399,346). The term “naked polynucleotide” refers to a polynucleotide that is not delivered with an agent that facilitates transfection. Naked polynucleotides are sometimes cloned into plasmid vectors. Such vectors can further contain a promoter, such as bupivacaine (W
einer et al. U.S. Pat. No. 5,593,972). DNA is
It can also be administered using a gene gun. Xiao & Brandsm
See the above reference for a. DNA encoding the immunogen is deposited on the surface of fine metal beads. The microprojectile is accelerated with shock waves or expanding helium gas and penetrates the tissue to several cell layers deep.
For example, Agricetus, Inc. Ac manufactured by Middletown WI
A cel ^™ gene transfer device is suitable. Alternatively, naked DNA can pass through the skin into the bloodstream by simply striking the DNA on the skin using chemical or mechanical stimuli (Howell).
et al. International Patent Publication (WO) 95/05853).

In further changes, the vector encoding the immunogen is delivered to ex vivo cells, such as cells explanted from individual patients (eg, lymphocytes, bone marrow aspirates, tissue biopsies) or universal donor hematopoietic stem cells, The cells can then be re-implanted into the patient after selection of cells that normally incorporate the vector.
II. Prophylactic and therapeutic methods The present invention provides, among other things, beneficial therapeutic responses within patients (eg, induction of phagocytosis of Aβ, reduction of plaque burden, inhibition of plaque formation, for prevention or treatment of amyloid disease, Under conditions that generate neuritic dystrophy, improve cognitive function, and / or reverse cognitive decline, treat or prevent) within the patient, the patient is treated with a therapeutic immunological reagent against a particular epitope within Aβ ( For example, treatment of Alzheimer's disease and other amyloid diseases by administration of humanized immunoglobulins). The present invention is also directed to the use of the disclosed immunological reagents (eg, humanized immunoglobulins) in the manufacture of a medicament for the treatment or prevention of amyloid diseases.

In one aspect, the present invention provides a method for preventing or treating a disease associated with amyloid deposition of Aβ in a patient's brain. Such diseases include Alzheimer's disease, Down's syndrome and cognitive impairment. The latter can occur with or without other characteristics of amyloid disease. Some methods of the invention comprise administering to a patient an effective dose of an antibody that specifically binds to one component of amyloid deposits. Such methods are particularly useful for preventing or treating Alzheimer's disease in human patients. An exemplary method comprises administering an effective dose of an antibody that binds to Aβ. A preferred method is to use residue 1 of Aβ
An antibody that specifically binds to an epitope within 10 to 10 such as an antibody that specifically binds to an epitope within residues 1 to 3 of Aβ, an antibody that specifically binds to an epitope within residues 1 to 4 of Aβ, A
An antibody that specifically binds to an epitope within residues 1 to 5 of β, an antibody that specifically binds to an epitope within residues 1 to 6 of Aβ, and specifically to an epitope within residues 1 to 7 of Aβ An antibody that binds,
Or administering an effective dose of an antibody that specifically binds to an epitope within residues 3-7 of Aβ. In yet another aspect, the invention features administering an antibody that binds to an epitope comprising the free N-terminal residue of Aβ. In yet another aspect, the invention features administering an antibody that binds to an epitope within 1 to 10 residues of Aβ, wherein residues 1 and / or 7 of Aβ is aspartic acid. In yet another aspect, the invention features administering an antibody that specifically binds to Aβ peptide without binding to full-length amyloid precursor protein (APP). In yet another aspect, the antibody isotype is human IgG1.

In yet another aspect, the invention features administering an antibody that binds to and induces elimination of a response to amyloid deposits in a patient. For example, such a removal reaction can be performed by Fc receptor-mediated phagocytosis.

The therapeutic agents of the present invention are typically pure from unwanted inclusions. this is,
It means that the agent is typically at least about 50% w / w (weight / weight) pure and essentially free of interfering proteins and contaminants. Sometimes the agent is at least about 80% w / w, and more preferably at least 90 or about 95% w / w pure. However, using conventional protein purification techniques, homogeneous peptides that are at least 99% w / w pure can be obtained.

The method can be used for both asymptomatic patients and patients currently presenting with symptoms of the disease.
The antibodies used in such methods can be human, humanized, chimeric or non-human antibodies, or fragments thereof (eg, antigen binding fragments), and can be monoclonal or polyclonal as described herein. Can be. In yet another aspect, the invention features administering an antibody prepared from a human immunized with an Aβ peptide, wherein the human can be a patient treated with the antibody.

In another aspect, the invention features administering an antibody with a pharmaceutical carrier as a pharmaceutical composition. Alternatively, the antibody can be administered to a patient by administering a polynucleotide encoding at least one antibody chain. The polynucleotide is expressed in a patient to produce an antigen chain. Optionally, the polynucleotide encodes the heavy and light chains of the antibody. The polynucleotide is expressed in the patient to produce heavy and light chains. In an exemplary embodiment, the patient is monitored for the level of antibody administered into the patient's blood.

Thus, the present invention satisfies the long-standing need for neuropathology and, in some patients, treatment regimens for preventing or reducing cognitive impairment associated with Alzheimer's disease.
A. Patients who are amenable to treatment Patients who are amenable to treatment include individuals who are at risk for disease but who are not symptomatic, as well as patients who are currently symptomatic. In the case of Alzheimer's disease, virtually all people are at risk of suffering from Alzheimer's disease if they live long enough. Thus, the method can be applied prophylactically to the general population without requiring any assessment of the subject patient's risk. This method is particularly useful for individuals who are known for a known genetic risk of Alzheimer's disease. Such individuals include those with relatives who have experienced the disease and those whose risk has been determined by analysis of genetic or biochemical markers. Genetic markers of risk for Alzheimer's disease include mutations in the APP gene, particularly mutations at positions 717 and 670 and 671, referred to as Hardy and Swedish mutations, respectively (see Hardy, supra). Other markers of risk are the presenilin gene, PS
1 and PS2 mutations, and family history of ApoE4, AD, hypercholesterolemia or atherosclerosis. Individuals currently suffering from Alzheimer's disease can be recognized from characteristic dementia, as well as the presence of the risk factors described above. In addition, a number of diagnostic tests are available to identify individuals with AD. This includes measuring CFS tau and Aβ42 levels. Elevated tau and lowered Aβ42 levels indicate the presence of AD. Individuals suffering from Alzheimer's disease can also be diagnosed by ADRDA criteria, as discussed in the Examples section.

In asymptomatic patients, treatment can begin at any age (eg, 10, 20, 30). Usually, however, it is not necessary to begin treatment until the patient reaches 40, 50, 60 or 70. Treatment typically involves multiple administrations over a period of time. Treatment can be monitored by assessing antibody levels during the period. If the response decreases, an additional dose is indicated. In the case of a potential Down syndrome patient, treatment can be initiated to the mother before delivery or by administration of a therapeutic agent immediately after delivery.
B. In treatment regimes and dose- preventive applications, the pharmaceutical composition or medicament may be used to treat biochemical, histological and / or behavioral symptoms of the disease in patients suspected or at risk of Alzheimer's disease, It is administered in an amount sufficient to eliminate or reduce disease risk, reduce severity, or delay onset, including its complications and intermediate pathological phenotypes that appear during disease progression. In therapeutic applications, patients who are suspected or already suffering from such a disease may be intermediated between complications of the disease (biochemical, histological and / or behavioral) and disease progression. The composition or medicament is administered in an amount sufficient to cure or at least partially block disease symptoms, including a pathological phenotype.

In some methods, administration of the drug reduces or eliminates myocognitive impairment in patients who have not yet developed a characteristic Alzheimer's disease pathology. An amount adequate to effect a therapeutic or prophylactic treatment is defined as a therapeutically or prophylactically effective amount. In both prophylactic and therapeutic regimes, agents are usually administered in several doses until a sufficient immune response is reached. The term “immune response” or “immunological response” refers to humoral (antibody-mediated) and / or cellular (antigen-specific T cells or their secreted products) directed against an antigen within a recipient subject. Including the occurrence of reactions). Such a response can be induced by an active response, ie, a response induced by administration of an immunogen, or a passive response, ie, administration of an immunoglobulin or antibody or antigen-stimulated T cell. Typically, the immune response is monitored and repeated doses are given when the immune response begins to weaken.

Effective dosages of the compositions of the invention for the treatment of the above-mentioned pathologies include the means of administration, the target site, the patient's physiological condition, whether the patient is a human or animal, other pharmaceuticals to be administered, and It varies depending on a number of different factors including whether the treatment is prophylactic or therapeutic. Normal,
The patient is a human but non-human mammals including transgenic mammals can also be treated. Treatment doses must be titrated to be safe and optimize effectiveness.
For passive immunization with antibodies, the dosage is about 0.0001 to
100 mg / kg, and more usually 0.01-5 mg / kg (eg 0.02 mg
/ Kg, 0.25 mg / kg, 0.5 mg / kg, 0.75 mg / kg, 1 mg / kg,
2 mg / kg). For example, the dosage is 1 mg / kg (body weight) or 1
Within the range of 0 mg / kg (body weight) or 1-10 mg / kg, preferably at least 1 mg
/ Kg. In another example, the dosage is 0.5 mg / kg (body weight) or 1
Within the range of 5 mg / kg (body weight) or 0.5-15 mg / kg, preferably at least 1
It can be mg / kg. Intermediate doses within the above ranges are also considered within the scope of the present invention.
Subjects can be administered such doses daily, every other day, weekly or according to any other schedule determined by empirical analysis. Exemplary treatments include dosing in multiple doses over an extended period of time, eg, for at least 6 months. Another exemplary treatment regime includes dosing once every two weeks or once every month or once every 3 to 6 months. An exemplary dosing schedule is:
Consecutive daily 1-10 mg / kg or 15 mg / kg, every other day 30 mg / kg or weekly 60 mg / kg. In some methods, two or more monoclonal antibodies having different binding specificities are administered simultaneously, in which case the dosage of each antibody dose is within the indicated range.

Antibodies are usually administered on multiple occasions. The interval between single doses can be weekly, monthly or yearly. Intervals can also be irregular as indicated by measuring blood levels of antibodies to Aβ within the patient. In some methods, the dosage is 1-1000 μg.
/ Ml, and in some methods, adjusted to achieve a plasma antibody concentration of 25-300 μg / ml. Alternatively, the antibody can be dosed as a sustained release formulation, in which case less frequent dosing is required. Dosage and frequency will vary depending on the half-life of the antibody within the patient. In general, humanized antibodies show the longest half life, followed by chimeric antibodies and nonhuman antibodies.

The dosage and frequency of dosing can vary depending on whether the treatment is prophylactic or therapeutic. For prophylactic applications, the composition comprising the antibody or a cocktail thereof is dosed to a patient who is not yet affected to enhance patient resistance. Such an amount is defined as a “prophylactic effective dose”. In this use, the exact amount will again depend on the patient's health and general immunity, but generally 0.1-25 mg per dose, especially 0 per dose.
. It is in the range of 5-2.5 mg. Relatively low doses are dosed at relatively infrequent intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives.

In therapeutic applications, relatively high doses (e.g., per dose) until disease progression slows or stops, and preferably until the patient shows partial or complete improvement in disease symptoms. A dose of about 1 to 200 mg, more usually 5 to 25 mg of antibody is sometimes required). Thereafter, the patient can be dosed in a prophylactic manner.

The dosage of nucleic acid encoding the antibody is about 10 ng to 1 g, 100 ng to 10 ng per patient.
It is in the range of 0 mg, 1 μg to 10 mg or 30 to 300 μg DNA. The dose of infectious viral vector varies from 10 to 100 or more virions per dose.

The therapeutic agent can be administered by parenteral, topical, intravenous, oral, subcutaneous, intraarterial, intracranial, intraperitoneal, intranasal or intramuscular means for prophylactic and / or therapeutic treatment. The most typical route of administration of immunogenic agents is subcutaneous, but other routes are equally effective. The next most common route is intramuscular injection. This form of injection is most typically performed in the arm or leg muscles. Some methods are direct injection of the drug into certain tissues where deposits have accumulated, such as intracranial injection. Intramuscular injection or intravenous infusion is preferred for administration of the antibody. In some methods, specific therapeutic antibodies are injected directly into the cranium. In some methods, antibodies are administered as a sustained release composition or as a device, eg, a Medipad ^™ device.

The agents of the present invention can optionally be administered in combination with other agents that are at least partially effective in the treatment of amyloid diseases. In certain embodiments, humanized antibodies of the invention (eg, humanized 12
A11) is administered in combination with a second immunogenic or immunological agent. For example, the humanized 12A11 antibody of the present invention can be administered in combination with other humanized antibodies against Aβ. In other embodiments, the humanized 12A11 antibody is administered to a patient who has been or has been administered an Aβ vaccine. In the case of Alzheimer's disease and Down's syndrome, where amyloid deposition occurs in the brain, the agent of the present invention can be administered together with other agents that increase the passage of the agent of the present invention across the blood brain barrier. The agents of the present invention can be administered in combination with other agents that enhance the access of the therapeutic agent to target cells or tissues, such as liposomes. Co-administration of such agents is necessary for the therapeutic agent (eg, therapeutic antibody or antibody chain) necessary to achieve the desired effect.
Can be reduced.
C. Pharmaceutical Compositions Agents of the invention are often administered as a pharmaceutical composition comprising an active therapeutic agent and various other pharmaceutically acceptable ingredients. Remington's Phar
material Science (15th edition, Mack Publishing)
See Company, Easton, Pennsylvania (1980). The preferred dosage form depends on the intended mode of administration and therapeutic application. The composition may also include a pharmaceutically acceptable non-toxic carrier or diluent, depending on the desired formulation, which is usually used to formulate a pharmaceutical composition for animal or human administration. Defined as the vehicle used. The diluent is selected so as not to affect the biological activity of the combination. Examples of such diluents are distilled water, physiological phosphate buffered saline, Ringer's solution, dextrose solution, and Hank's solution. In addition, the pharmaceutical composition or formulation may include other carriers, adjuvants, or nontoxic, nontherapeutic, nonimmunogenic stabilizers and the like.

The pharmaceutical compositions also include proteins, polysaccharides such as chitosan, polylactic acid, polyglycolic acid and copolymers (eg latex functionalized Sepharose ™, agarose, cellulose, etc.), polymeric amino acids, amino acid copolymers, and It can contain large and slowly metabolized macromolecules such as lipid aggregates (eg oil droplets or liposomes). In addition, these carriers are immunostimulatory agents (ie, adjuvants)
Can function as.

For parenteral administration, the agents of the invention are in a physiologically acceptable diluent with a pharmaceutical carrier, which can be a sterile liquid such as water, oil, saline, glycerol, or ethanol. It can be administered as an injectable dose of a solution or suspension of the substance. In addition, auxiliaries such as wetting or emulsifying agents, surfactants, pH buffering substances and the like can also be present in the composition. Other components of the pharmaceutical composition are of petroleum, animal, vegetable, or synthetic origin, such as peanut oil, soybean oil and mineral oil. In general, glycols such as propylene glycol or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions. The antibodies can be administered in a depot or implanted dosage form, which can be formulated in a manner to allow sustained release of the active ingredient. An exemplary composition is 50 mM L-
5 mg / mL monoclonal antibody formulated in a buffered aqueous solution consisting of histidine, 150 mM NaCl and adjusted to pH 6.0 using HCl.

Typically, the compositions are manufactured as either injectable liquid solutions or suspensions, but pre-injection solid dosage forms suitable for solutions or suspensions in liquid vehicles can also be manufactured. The formulation can also be emulsified or encapsulated in liposomes or microparticles such as polylactide, polyglycolide, or copolymers for enhanced adjuvant effect as discussed above. (Langer, Science 249: 1527 (1990) and H
anes, Advanced Drug Delivery Reviews 28: 9
7 (1997)). The agents of the present invention can be administered in the form of a depot infusion or implantable formulation, which can be formulated in a manner that allows sustained or pulsatile release of the active ingredient.

Additional formulations suitable for other modes of administration include oral, nasal and pulmonary formulations, suppositories, and transdermal applications. For suppositories, binders and carriers include, for example, polyalkylene glycols or triglycerides, such suppositories are 0.5% to 10%, preferably 1% to 2%.
Can be formed from mixtures containing active ingredients within the range of Oral formulations include excipients such as pharmaceutical grade mannitol, lactose, starch, magnesium stearate, sodium saccharin, cellulose, and magnesium carbonate. These compositions are solutions, suspensions,
Take the form of tablets, pills, capsules, sustained release formulations or powders, and 1 active ingredient
0 to 95%, preferably 25% to 70%.

Topical application can result in transdermal or intradermal delivery. Topical administration can be facilitated by co-administration of the drug with cholera toxin or its detoxified derivatives or their subunits or other similar bacterial toxins (Glenn et al., Natu).
re 391: 851 (1998)). Co-administration can be achieved by using the components as a mixture or as a linking molecule obtained by chemical cross-linking or expression of a fusion protein.

Alternatively, transdermal delivery can be accomplished using skin patches or transferosomes (trans
(Paul et al., Eur. J. Imm)
unol. 25: 3521 (1995); Cevc et al. Biochem. B
iophys. Acta 1368: 201-15 (1998)).
D. Monitoring the progress of treatment The present invention provides a method for monitoring a treatment within a patient suffering from or suspected of Alzheimer's disease, i.e., monitoring the progress of a treatment given to a patient. The method can be used to monitor both therapeutic treatment for symptomatic patients and prophylactic treatment for asymptomatic patients. in particular,
The method is useful for monitoring passive immunization (eg, measuring the level of antibody administered).

Some methods include, for example, measuring an antibody level or profile reference value in a patient and comparing it to a post-treatment profile or level value prior to administering a dose of the drug. A significant increase in level or profile value (ie, greater than the typical limit of experimental error in repeated measurements of the same sample, expressed as one standard deviation from the mean of such measurements) indicates a positive treatment result ( That is, drug administration achieved the desired response). A negative treatment result is indicated if the value of the immune response does not change significantly or decreases.

In another method, level or profile control values (ie, mean and standard deviation) are determined relative to a control population. Typically, individuals in the control population have not received pretreatment. The measured level or profile within the patient after administration of the therapeutic agent is then compared to a control value. A significant increase (eg, more than one standard deviation from the mean) compared to the control value indicates a positive or satisfactory treatment result. A lack or decrease in significant increase indicates a negative or insufficient treatment outcome. Administration of the drug is generally continued while the level increases relative to the control value. As before, stable achievement relative to the control value is an indication that treatment delivery can be interrupted or dose and / or frequency can be reduced.

In another method, level or profile control values (eg, mean and standard deviation) are determined from a control population of individuals who have been treated with a therapeutic agent and whose level or profile is stable in response to treatment. . The measured level or profile within the patient is compared to the control value. Treatment can be interrupted if the measured value in the patient does not differ significantly (eg, greater than one standard deviation) from the control value. If the level within the patient is significantly lower than the control value, continuous administration of the drug is justified. If the level in the patient is persistently lower than the control value,
A change of treatment may be instructed.

In another method, patients who are not currently receiving treatment but have previously received a course of treatment are monitored for antibody levels or profiles to determine if treatment resumption is necessary. The measurement level or profile within the patient can be compared to values previously reached in the patient after the previous course of treatment. A significant decrease relative to the previous measurement (ie, exceeding the typical range of errors in repeated measurements of the same sample) is an indicator that treatment can be resumed. Alternatively, the value measured within the patient can be compared to a control value (mean plus standard deviation) determined within the patient population after undergoing a course of treatment. Alternatively, the value measured within a patient can be compared to a control value within a population of prophylactically treated patients that does not exhibit disease symptoms, or a population of therapeutically treated patients that exhibit improved disease characteristic values. In all these cases, a significant decrease relative to the control value (ie, exceeding one standard deviation) is an indication that treatment should be resumed within the patient.

The tissue sample for analysis is typically blood, plasma, serum, mucus or cerebrospinal fluid from a patient. The sample is analyzed, for example, for the level or profile of antibodies against Aβ peptide, eg, the level or profile of humanized antibodies. An ELISA method for detecting antibodies specific for Aβ is described in the Examples section. In some methods, as described herein, the level or profile of the administered antibody is eliminated by an assay such as in
Determine using in vitro phagocytosis assay. In such a method, a tissue sample from the patient to be tested is contacted with amyloid deposits (eg, from PDAPP mice) and phagocytes carrying Fc receptors. The subsequent removal of amyloid deposits is then monitored. The presence and extent of the ablation response provides an indication of the presence and level of antibody effective to remove Aβ in the tissue sample of the patient being tested.

The antibody profile after passive immunization typically shows an immediate peak in antibody concentration and a subsequent exponential drop. Without further administration, the reduction approaches pre-treatment levels within a period of days or months depending on the half-life of the administered antibody.

In some methods, a baseline measurement of antibodies to Aβ in a patient is made prior to administration, a second measurement is taken immediately thereafter to determine peak antibody levels, and one or more additional measurements This is done at intervals to monitor the drop in level. An additional dose of antibody is administered when the level of antibody drops to a predetermined percentage of the difference between baseline or peak and baseline (eg, 50%, 25% or 10%). In some methods, the peak or subsequent measurement level minus the background is compared to a previously determined reference level that constitutes a beneficial prophylactic or therapeutic treatment regime in other patients. If the measured antibody level is significantly lower than the reference level (eg, lower than the mean of the reference values within the population of patients benefiting from treatment minus one standard deviation), an additional dose of antibody An amount of administration is indicated.

Additional methods include any art-recognized physiological condition (e.g., physical body) that is routinely trusted by researchers or physicians who diagnose or monitor amyloid diseases (e.g., Alzheimer's disease) during the course of treatment. Monitoring of mental or psychological symptoms). For example, cognitive impairment can be monitored. The latter is a symptom of Alzheimer's disease and Down's syndrome but can occur without any other features of those diseases. For example, cognitive impairment may occur in a mini-mental state test (Mini-Mental St
The patient's score can be determined and monitored with “ate Exam”.
E. Kit The present invention further provides a kit for carrying out the monitoring method described above. Typically, such kits contain an agent that specifically binds to an antibody to Aβ. The kit is labeled
) Can also be included. For detection of antibodies to Aβ, the label is typically in the form of a labeled anti-idiotype antibody. For antibody detection, the drug can be pre-coupled to a solid phase, eg, a well of a microtiter dish. The kit also typically includes labeling that provides instructions for using the kit. Labeling may include a chart or other corresponding scheme that correlates the measured level of label with the level of antibody to Aβ. The terminology for labeling is
Refers to any written or recorded material that is affixed to or otherwise accompanied by the kit at any time during manufacture, transportation, sale, or use of the kit. For example, labeling terms include advertising brochures and booklets, packaging materials, instructions, audio or video cassettes, computer discs, and descriptions directly engraved on kits.

The present invention relates to diagnostic kits, such as research, detection and / or diagnostic kits (eg i
to perform n vivo imaging). Such kits are typically
Preferably, an antibody that binds to an epitope of Aβ within residues 1-10 is included. Preferably, the antibody is labeled or a secondary labeling reagent is included in the kit. Preferably, the kit is labeled with instructions for carrying out the intended use, for example for carrying out in vivo imaging assays. Exemplary antibodies are those described herein.
F. In vivo imaging The present invention provides a method for in vivo imaging of amyloid deposits in a patient. Such methods are useful for diagnosing or confirming Alzheimer's disease, or its susceptibility. For example, the method can be used for patients who exhibit symptoms of dementia. If the patient has abnormal amyloid deposits, the patient may be suffering from Alzheimer's disease. The method can also be used for asymptomatic patients. The presence of abnormal amyloid deposits
Shows susceptibility to future symptomatic diseases. The method is also useful for monitoring disease progression and / or response to treatment in patients previously diagnosed with Alzheimer's disease.

The method works by administering to the patient an antibody that binds to an agent, eg, Aβ, and then detecting the agent after the binding. Preferred antibodies do not bind to the full length APP polypeptide but bind to Aβ deposits in the patient. Antibodies that bind to an epitope of Aβ within amino acids 1-10 are particularly preferred. In some methods, the antibody binds to an epitope within amino acids 7-10 of Aβ. Such antibodies typically bind without inducing an intrinsic elimination reaction. In another method, the antibody binds to an epitope within amino acids 1-7 of Aβ. Such antibodies typically bind to Aβ and induce a clearing reaction. However, the elimination reaction can be avoided using an antibody fragment that lacks the full-length constant region, eg, Fab. In some methods, the same antibody serves as both a treatment and diagnostic reagent. In general, an antibody that binds to an epitope from the C-terminus of Aβ to residue 10 does not show as strong a signal as an antibody that binds to an epitope within residues 1-10, but presumably C- This may be because the terminal epitope is not accessible within the amyloid deposit. Therefore, such antibodies are less preferred.

Diagnostic reagents can be administered directly into the patient's body by intravenous infusion, or by intracranial infusion or through a hole in the skull. The dosage of the reagent should be within the same range as the treatment method. Typically, the reagent is labeled, but in some methods the primary reagent with affinity for Aβ is not labeled and a secondary labeling reagent is used to bind to the primary reagent. The choice of label depends on the means of detection. For example, fluorescent labels are suitable for optical detection. The use of paramagnetic labels is suitable for tomographic detection without surgical invasion. A radioactive label can also be detected using PET or SPECT.

Diagnosis is performed by comparing the number, size, and / or intensity of labeled loci with corresponding reference values. The reference value can represent an average level within a population of unaffected individuals. The reference value can also represent a previous level determined within the same patient. For example, the reference value is
The value can be determined within the patient prior to the start of treatment, and then the measured value is compared to a reference value. A decrease in value relative to the baseline indicates a positive response to treatment.

  The invention is further described by the following non-limiting examples.

The following sequence identifiers are used throughout all of the examples referring to immunoglobulin chain variable region nucleotide and amino acid sequences.

As used herein, an antibody or immunoglobulin sequence comprising a VL and / or VH sequence set forth in any one of SEQ ID NOs: 1-4 can comprise a full-length sequence or Either can comprise a mature sequence (ie, a mature peptide without a signal or leader peptide).

Previous studies have shown that various Aβ antibodies that reduce AD-related neuropathology (eg, plaque burden) in
Vivo efficacy is ex vivo (ex vivo) (eg in PDAPP or AD brain sections)
Bind plaques and / or ex vivo phagocytosis assay (Bard et
al. (2000) Nat. Med. 6: 916-919) showed that it can be predicted by the ability of the antibody to trigger plaque removal. This correlation supports the opinion that Fc-dependent phagocytosis by microglia and / or macrophages is important in the process of plaque removal in vivo. However, it has also been reported that antibody efficacy can be obtained in vivo by a mechanism independent of Fc interaction (Bacskai et al., (
2002) J. et al. Neurosci. 22: 7873-7878). Studies have shown that antibodies that specify an intermediate portion of Aβ that cannot recognize amyloid plaques bind to soluble Aβ and reduce plaque deposition (DeMattos et al. (2001) Proc. Natl.
. Acad. Sci. USA 98: 8850-8855).

Potential in vivo of murine monoclonal antibody 12A11 (isotype IgG1)
o Various ex vivo assays were first performed to characterize efficacy.

Binding activity (activity) of mAb12A11 to Aβ1-42 The binding of monoclonal antibody 12A11 to aggregated synthetic Aβ1-42 was determined by Schenk et al.
. (Nature 400: 173 (1999)). For comparison purposes, mAbs 12B4 and 10D5 were also assayed. Soluble Aβ1
-42 refers to a synthetic Aβ1-42 peptide sonicated in dimethyl sulfoxide (DMSO). Serial dilutions of antibody at 20 μg / ml were 50,000 cpm [ ¹²⁵ I
] Aβ 1-42 (190 μCi / μmol; labeled with Indogen reagent Pierce) was incubated overnight at room temperature. 75 mg / ml protein A Se
50 μL of a slurry containing pharose (Amersham Pharmacia) and 200 μg rabbit anti-mouse IgG (H + L) (Jackson ImmunoResearch) was incubated with diluted antibody for 1 hour at room temperature, washed twice, and a Wallac gamma counter (Perkin -Elmer).
All steps were performed in RIA buffer consisting of 10 mM Tris, 0.5 M NaCl, 1 mg / ml gelatin, and 0.5% Nonidet P-40, Ph 8.0.

  The results from the binding activity study are shown in Table 2 below.

All antibodies tested showed high binding activity against aggregated Aβ1-42. further,
Antibodies 12B4 and 12A11 captured soluble Aβ1-42 in an easily detectable manner at an antibody concentration of 20 μg / ml. As shown in Table 2, IgG1 antibody 12A11 is IgG2
Aβ1-42 was captured more efficiently than a antibody 12B4 or IgG1 antibody 10D5.

The ability of various antibodies (including 12A11) to capture soluble Aβ was further evaluated as follows. Various concentrations of antibody (up to 10 μg / ml) were incubated with 50,000 CPM of ¹²⁵ I-Aβ1-42 (or ¹²⁵ I-Aβ1-40).
The concentration of antibody sufficient to bind 25% of the radioactivity count was determined with a capture radioimmunoassay. For antibodies that are not capable of binding 25% of the count at 10 μg / ml, 10
The percentage of counts bound at μg / ml was measured. 12A11 bound 20% of the radioactivity count (ie ¹²⁵ I-Aβ) at 10 μg / ml. This is the other two types of A tested
Greater than the amount bound by β3-7 antibodies, 12B4 and 10D5 (7 μg / ml bound 7% and 2%, respectively). Thus, the tested N-terminus (epitope Aβ
3-7) Among the antibodies, 12A11 showed the most remarkable ability to capture Aβ.

Antibodies were also compared in an ex vivo phagocytosis assay using sections of brain tissue from primary mouse microglia and PDAPP mice as a measure of their ability to trigger Fc-mediated plaque removal. Irrelevant IgG1 and IgG2a antibodies that are not reactive to Aβ or other components of the assay were used as isotype matched negative controls.
In summary, murine primary microglia were cultured in the presence of antibodies with non-fixed cryostat sections of PDAPP mouse brain. After 24 hours of incubation, the total level of Aβ remaining in the culture was measured by ELISA. To quantify the extent of plaque removal / Aβ degradation, Aβ was extracted from cultures of microglia and brain sections (n = 3) for ELISA testing using 8M urea. Data were analyzed by ANOVA followed by a post Dunnett test.

As shown in FIG. 1, the 12B4 antibody effectively reduces Aβ levels (73% for 12B4, P <0.001), while 12A11 shows somewhat lower but statistically significant lower efficiency (48 for 12A11). %, P <0.05). The 10D5 antibody did not significantly reduce Aβ levels. The performance of 12A11 in an ex vivo phagocytosis assay will be improved upon conversion to the IgG2a isotype, which is the preferred isotype for microglia phagocytosis.
Example II

In vivo efficacy of mouse 12A11 antibody Mouse antibody 12A11 reduces Alzheimer's disease-like neuropathology in vivo.
To determine the in vivo potency of 12A11, antibodies (including 12A11, 12B4, or 10D5) can be obtained according to Bard et al. (2000) Nat. Med. 6:91
Mice were administered 10 mg / kg by weekly intraperitoneal injection for 6 months as described in 6. At the end of the study, the total level of cortical Aβ was determined by ELISA. As shown in FIG. 2A, each antibody significantly reduced Aβ levels compared to the PBS control (P <0.
001), i.e. 12B4 showed a 69% reduction, 10D5 showed a 52% reduction, and 12A11 showed a 31% reduction.

The level of neuritic dystrophy was then tested in sections of brain tissue from the mice to determine the association between plaque removal and neuronal protection. Data from a brain image analysis examining the proportion of frontal cortex occupied by neuritic dystrophy is shown in FIG. 2B.
These data indicate that antibodies 10D5 and 12A11 are not very effective at reducing neuritic dystrophy, as determined by the assays described herein, while 12B4 significantly reduces neuritic dystrophy (12B4 , P <0.05; ANOVA, followed by post hoc Dunnett test). Again, the affinity of 12A11 is 12A
It will be improved by converting 11 to the IgG2a isotype (murine potency). 12A11
For the humanized version of, the IgG2a isotype is preferred for reducing neuritic dystrophy.

Experiments demonstrating the binding properties and in vivo efficacy of antibody 12A11 are described in Bard, e
t al. PNAS 100: 2023 (2003), which is also incorporated herein by reference.

In summary, all antibodies have significant binding activity to aggregated Aβ and ex
Trigger plaque removal with the in vivo assay. The IgG2a isotype (affinity for Fc receptors, particularly FcγRI) appears to be an important attribute for both the removal of Aβ and protection against neuritic dystrophy. Antibody 12A11 (IgG1) is 12B4 (IgG2a)
Or capture soluble monomeric Aβ1-42 with higher efficiency than 10D5 (IgG1), but is less effective in reducing neuritic dystrophy. High efficacy in improving plaque burden and reducing neuritic dystrophy would be achieved by engineering the antibody to have an isotype that best supports phagocytosis. Particularly effective are antibodies that bind to an epitope within the N-terminus of Aβ.
Example III

Cloning and sequencing of the murine 12A11 variable region
Cloning and sequence analysis of 12A11VH 12A11 from hybridoma cells
The VH and VL regions are cloned by RT-PCR and 5'RACE using mRNA and standard cloning methods from hybridoma cells. Inferred 12A
The nucleic acid sequences (coding, SEQ ID NO: 3) and deduced amino acid sequences (SEQ ID NO: 4) derived from independent cDNA clones encoding the 11 VH domain are shown in Tables 3 and 4, respectively.

Cloning and sequence analysis of 12A11 VL
Cloned in the same manner as the H region. Nucleotide sequences derived from two independent cDNA clones encoding the putative 12A11 VL domain (coding,
SEQ ID NO: 1) and deduced amino acid sequence (SEQ ID NO: 2) are shown in Table 5 and Table 6, respectively.
Shown in

The 12A11 VL and VH sequences are functional V region reference as long as they contain a contiguous ORF from the initiator methionine to the C-region and share the conserved residues characteristic of immunoglobulin V region genes. Fits. From the N-terminus to the C-terminus, both the light chain and the heavy chain comprise the domains FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4.
Example IV

Expression of chimeric 12A11 antibody
The expression variable heavy and light chain regions of chimeric 12A11 are reengineered to encode a splice donor sequence downstream of the respective VDJ or VJ junction, and the heavy chain contains pC
MV-hγ1, and the light chain was cloned into a mammalian expression vector of pCMV-hκ1. These vectors encode human γ1 and Cκ constant regions as exonic fragments downstream of the inserted variable region cassette. Following sequence verification, heavy and light chain expression vectors were cotransfected into COS cells. Different heavy chain clones are co-transfected independently with different chimeric light chain clones to confirm the reproducibility of the results. The antibody is COS using protein A Sepharose.
Immunoprecipitated from cell conditioning media. The antibody chain was detected on an immunoblot of an SDS-PAGE gel. Detection was achieved using a goat-anti-human IgG (H + L) antibody at a 1: 5000 dilution at room temperature for 1 hour. A significant amount of 12A11 H + L chain was detected in the conditioning medium.

Direct binding of the chimeric 12A11 antibody to Aβ was tested by ELISA assay.
FIG. 4 demonstrates that chimera 12A11 was found to bind to Aβ with high avidity, similar to that demonstrated by chimeric and humanized 3D6. (3D6
Cloning, characterization and humanization are described in US patent application Ser. No. 10 / 010,942, the entire contents of which are incorporated herein by reference).
The binding activity was similar to that demonstrated by chimeric and humanized 12B4 (12
Cloning, characterization and humanization of B4 is described in US patent application Ser. No. 10 / 388,21.
No. 4, the entire contents of which are incorporated herein by reference).
Example V

12A11 humanization
A. 12A11 humanized antibody, version 1
Homology / Molecular Model Analysis In order to identify the key structural framework residues within the murine 12A11 antibody, a three-dimensional model for the elucidated murine antibody with homology to the 12A11 heavy and light chains was studied. An antibody called 1KTR with close homology to the 12A11 light chain is selected and 1ETZ and 1JR with close homology to the 12A11 heavy chain
Two antibodies designated H were selected. These murine antibodies show strong sequence conservation with 12A11 (93% identity within 112 amino acids to Vk and 1 each to Vh
83% identity for 26 amino acids and 86% identity for 121 amino acids). 1E
The heavy chain structure of TZ was superimposed on that of 1KTR. In addition, the C of the selected antibody against Vk
The DR loop belongs to the same regular Chothia structure classification, and so does the CDR loop of 12A11VL. The crystal structures of these antibodies are similar to the 12A
Residues predicted to be important for the function of the 11 antibody (for example, FR residues important for CDR conformation, etc.) were examined.

Selection of human acceptor antibody sequences Appropriate human acceptor antibody sequences were identified by computer comparison of the amino acid sequence of the mouse variable region with the sequence of known human antibodies. Comparisons were made separately for 12A11 heavy and light chains. In particular, variable domains from human antibodies whose framework sequences show a high degree of sequence identity with the murine VL and VH framework regions can be obtained using NCB1 BLAST (Nati
Identified by searching the NCBI Ig database using public Institute of Health NCBI Internet server).

The following criteria: (1) homology with the subject sequence, (2) sharing the canonical CDR structure with the donor sequence, and / or (3) not including any rare amino acid residues within the framework region Based on this, two candidate sequences were selected as acceptor sequences. The acceptor sequence selected for VL was BAC01733 in the NCBI Ig non-redundant database. The acceptor sequence selected for VH was AAA69734 in the NCBI Ig non-redundant database. AAA69734 is a human subgroup III antibody (rather than subgroup II), but Saldanha et al. , (199
9) Mol. Immunol. Selected as the initial acceptor antibody based at least in part on the estimation of 36: 719. The first version of the humanized 12A11 antibody utilized their selected acceptor antibody sequence. Antibody is Schroeder and
Wang (1990) Proc. Natl. Acad. Sci. USA 872: 614
6 is described.

As described above, the humanized antibody of the present invention comprises a variable framework region derived essentially from a human immunoglobulin (acceptor immunoglobulin), and a mouse immunoglobulin (donor designated 12A11). Complementarity-determining regions derived essentially from immunoglobulins). Identifying the complementarity determining region of 12A11 and the appropriate human acceptor immunoglobulin, the next step is from those components to replace, if present, to optimize the properties of the resulting humanized antibody. Was the determination of residues.
Reshaped light chain V region :
The amino acid alignment of the reshaped light chain V region is shown in FIG. 5A. The choice of acceptor framework (BAC01733) is from the same human subgroup as that corresponding to the murine V region, has no unusual framework residues, and the CDRs are the same Choth.
It belongs to ia canonical structure group. No back mutations were made within version 1 of humanized 12A11.
Reshaped heavy chain V region:
The amino acid alignment of the reshaped heavy chain V region is shown in FIG. 5B. The selection of acceptor framework (AAA69734) is from human subgroup III (as described previously),
And it has no unusual framework residues. Structural analysis of the murine VH chain (1ETZ and 1JRH) associated with the amino acid alignment of AAA69734 to the murine sequence showed that 9 backmutations within version 1 (v1) of the reshaped heavy chain: A24F, T28S, F29L, V3
7I, V48L, F67L, R71K, N73T, and L78V (Kabat numbering) are designated. Back mutations are highlighted with an asterisk in the amino acid alignment shown in FIG. 5B.

Of the nine backmutations, three are specified by the model because they are proximal to canonical residues (A24F, F29L and R71K, white on black), ie CDR residues This is because it is a framework residue that is thought to contribute to antigen binding. There is one backmutation in the next most important class of residues, the interface residue or V37I is VH-
Involved in VL filling interactions (underlined). The N73T mutation is a vernier residue at the end of the binding site (surrounded by a dotted line) and probably interacts with S30 adjacent to CDR1. 4 left
Residues (T28S, V48L, F67L, L78V, Kabat numbering) are targets of backmutation and fall within the vernier classification (indirect contribution of CDR conformation, dotted line in FIG. 5B) Enclose).

  A summary of the changes incorporated into version 1 of humanized 12A11 is shown in Table 7.

Tables 8 and 9 show the key Kabat numbering for each of the various light and heavy chains.

The humanized antibody preferably exhibits a specific binding affinity for Aβ of at least 10 ⁷ , 10 ⁸ , 10 ⁹ or 10 ¹⁰ M ⁻¹ . Usually, the upper limit of the binding affinity of a humanized antibody for Aβ is within a factor of 3, 4 or 5 of that of 12A11 (ie about 10 ⁹ M ⁻¹ ). Often the lower limit of binding affinity is also within a factor of 3, 4 or 5 of that of 12A11.
Construction and expression PCR-mediated construction of humanized 12A11 VH and VL, version 1 is used to generate h12A11v1 with appropriate oligonucleotide primers. The nucleotide sequences of humanized 12A11VL (version 1) (SEQ ID NO: 34) and 12A11VH (version 1) (SEQ ID NO: 35) are shown in Tables 10 and 11 below, respectively.

The amino acid sequences of humanized 12A11VL (version 1) (SEQ ID NO: 7) and 12A11VH (version 1) (SEQ ID NO: 10) are shown in FIGS. 5A and 5B, respectively.
B. Humanized 12A11 antibody-versions 2, 2.1, and 3
Vernier residues (eg, S28T, V48L, F67L, L78V) are claimed to contribute indirectly to the CDR conformation and have minimal importance to conformational perturbation. Target residues were mutated by site-directed mutagenesis using Stratagene kits and h12A11VHv1 in the pCRS plasmid as a mutagenesis template to generate a clone corresponding to version 2. The V region insert confirmed by sequencing of version 2 was used as the BamHI / HindI of the heavy chain expression vector pCMV-Cgamma1.
Subcloning into the II site produced a recombinant h12A11v2 antibody. Version 2.1 antibodies were similarly created using each of the vernier residue mutations described above (ie, elimination of backmutation) in addition to the mutation at position T73N. Similarly, the version 3 antibody had the mutations T28S, L48V, L67F, and V78L, respectively, in addition to the mutation at position K71R.
C. Humanized 12A11 antibody, versions 4-6
The canonical and filling residues retain the back mutation, but one (version 4.
1-4.) 2 (versions 5.1-5.6) or 3 (versions 6.1-6.
An additional humanized 12A11 version was designed, excluding the vernier residue backmutation of 4). Site-directed mutagenesis and clone construction were performed as described in part C above. Recombinant antibody was expressed in COS cells and purified from COS cell supernatant. Additional versions include those described above, eg, at least one filling and / or canonical residue (eg, human residues at positions 28, 37, 48, 71 and 78 or positions 28, 37, 48, 6
Human residues at 1, 71, 73 and 78) in combination with 1, 2, 3, 4 or 5 vernier residues.
D. Humanized 12A11 antibody, versions 7 and 8
The 7th version of humanized 12A11 is the same for each reverse mutation shown in version 1 except for the T-> S back mutation at residue 28 (vernier) and the V-> I back mutation at residue 37 (filling). Created to have mutations. The eighth version of humanized 12A11 was created to have the respective back mutations shown in version 1, except for the N-> T back mutation (vernier) at residue 73. The amino acid sequences of humanized 12A11 version 7 and 8 heavy chains are shown in SEQ ID NOs: 30 and 31, respectively.

Compared to version 1, version 7 has only 7 mutations. The T28S backmutation is conserved and has been removed in heavy chain version 7. Filling residue V
The 37I back mutation has also been removed in version 7. Compared to version 1, version 7 has only 8 mutations. In version 8, N73T (Vernier)
Back mutations have been removed.

Additional versions may include combinations of the above, for example, optionally combined with the elimination of backmutations at at least one filling residue (eg position 37) and / or at least one canonical residue, Human residues at 1, 2, 3, 4 (or 5) residues selected from positions 28, 48, 78 and 73 (eg elimination of backmutation).
Example VI

Functional texturing of humanized 12A11 antibody
Humanized 12A11 version 1 was cloned as described in Example V.

Humanized 12A11 was produced by transient expression in COS cells and purified according to methodologies recognized in the art. The binding activity of the humanized antibody is first determined by qualitative ELIS.
Proved by A assay (data not shown). Humanized 12A11 version 1 and its murine and chimeric counterparts and two properties: antigen binding (quantitative Aβ
ELISA) and relative affinity were compared. The binding activity of h12A11v1 was demonstrated by quantitative Aβ ELISA and found to be identical to the murine and chimeric forms of 12A11 (see FIG. 7).

The affinity of h12A11v1 antibody was also determined by murine and chimeric 12 by competitive Aβ ELISA.
Comparison with A11 antibody. Biotin-conjugated recombinant mice 12 for competitive binding assays
A11Cγ2a (isotype switched 12A11) was used. The binding activity of biotinylated m12A11Cγ2a to aggregated Aβ1-42 was confirmed by an ELISA assay using streptavidin-HRP as a reporter. 12A11 using HRP-conjugated goat anti-mouse HRP as reporter
A direct comparison of Aβ binding by the two isoforms (Cγ1, Cγ2a) confirmed that the biotin-conjugated recombinant 12A11Cγ2a was equivalent to the original Cγ1 mouse antibody.

Competitive binding studies used biotin-conjugated m12A11Cγ2a at a fixed concentration and were competed over a range of concentrations of test antibody, as shown in FIG. FIG. 8 shows the results of an h12A11v1 competition assay comparing h12A11v1 with its chimeric and murine forms. Humanized 12A11v1 competed with its murine and chimeric counterparts within 2XIC50 values. This data is consistent with affinity determination using Biacore technology (data not shown)
It is 38 nM and 23 nM for murine Cγ2a and h12A11v1, respectively.
The KD value was shown. In summary, the findings suggest that h12A11v1 retains antigen binding and the affinity of its original murine counterpart.

COS cells were transiently transfected with various combinations of humanized 12A11VH and h12A11VLv1. Conditioned media was collected 72 hours after transfection. The antibody concentration in the conditioning medium from transfected COD cells is
Determined by quantitative human IgG ELISA. Quantitative Aβ (1-42) aggregate binding assay was performed with respect to antigen binding, h12A11v2, v2.1 and v3 were h12A11
Confirmed to be equivalent to v1 and to chimera 12A11. Moreover, versions 5.1-5.6 and 6.1-6.3 show similar binding activity when tested in this binding assay. Version 6.4 showed some loss of activity in the assay, but v2
The activity recovered significantly.

Binding to murine 12A11 and h12A11v1 was also compared using BIAcore technology. Murine 12A11 and h12A11v1 show similar binding profiles when exposed to Aβ peptide (bio-DAE peptide) immobilized at either low or high density. Kinetic analysis of murine 12A11 vs. h12A11v1 was also performed.
In those studies, BIAcore technology was used to measure the binding of soluble antibodies to solid phase bound biotinylated DAE peptides. The peptide was immobilized on a stratoatavidin biosensor chip, then varying concentrations of each antibody were applied in triplicate and binding was measured as a function of time. Data was analyzed using BIA evaluation software applied to the bivalent model. Apparent degradation (k _d ) and association (k _a ) rate constants were calculated from the appropriate area of the sensorgram using global analysis. The affinity constant for the interaction between bio-DAE10 and the antibody was calculated from the kinetic rate constant. From those measurements, apparent degradation (k _d ) and association (k _a ) rate constants were derived and used to calculate the _KD value for the interaction. Table 12 shows BIAcor
Includes summary of kinetic analysis of Aβ binding of 12A11 antibody as determined by e-analysis.

This data shows that humanized 12A11v1 compared to parenteral murine 12A11 when Aβ
Shows similar affinity for peptides.
Example VII

Prophylaxis and treatment of human subjects Single dose phase I studies are conducted to determine safety within humans. The therapeutic agent is about 0.01
Starting from the estimated level of potency, increasing by a factor of 3 is approximately 1
Different patients are given increasing doses until a 0-fold level is reached.

A phase II study is conducted to determine therapeutic efficacy. Select early and mid-stage Alzheimer's disease patients defined using the Alzheimer's Disease and Associated Disability Association (ADRDA) criteria for potential AD. Matching patients score in the 12-26 range in the Mini Mental Condition Test (MMSE). Another selection criterion is that there are no complications such as the possibility of patients surviving the duration of the study and the use of concomitant medications that would interfere. Baseline assessment of patient function is performed using classical psychopsychological metrics such as MMSE and ADAS, a comprehensive measure for assessing patients with Alzheimer's disease status and function. These psychometric measures provide a measure of the progression of Alzheimer's disease pathology. An appropriate qualitative life scale can also be used to monitor treatment. Disease progression is determined by MRI
Can also be monitored. The patient's blood profile can also be monitored, including immunogen-specific antibodies and T cell response assays.

Following the baseline measurement, the patient begins to receive treatment. Patients are randomized and treated with either therapeutic or placebo in a blinded manner. Patients are monitored at least every 6 months. Efficacy is determined by a significant decrease in progression of the treatment group relative to the placebo group.

A second phase II trial sometimes involves age-related memory impairment (AAMI) or mild cognitive impairment (MCI).
To assess patient transformation ranging from non-Alzheimer's early memory loss, referred to as)) to the possibility of Alzheimer's disease as defined by ADRA criteria. Reference population for early signs of memory loss or other difficulties associated with pre-Alzheimer symptoms, family history of Alzheimer's disease, genetic risk factors, age, gender, and other signs found to predict high risk of Alzheimer's disease To screen patients at high risk for conversion to Alzheimer's disease from nonclinical populations. In addition, other measures designed to assess normal populations are used together to collect baseline scores based on appropriate measures including MMSE and ADAS. These patient populations will be divided into appropriate groups for placebo comparisons to drug substitution groups using drugs. Their patient population is followed at approximately 6-month intervals and the end point of each patient is whether he or she is converted to putative Alzheimer's disease as defined by the ADRDA criteria at the end of the observation.

Although the foregoing invention has been described in detail for purposes of clarity of understanding, it will be apparent that certain modifications may be practiced within the scope of the appended claims. All publications and patents cited in this specification, as well as the text appearing in the figures and sequence listings, are hereby incorporated by reference in their entirety for all purposes to the same extent as if each was individually indicated. It is incorporated into the description.

From the foregoing, it will be apparent that the invention provides numerous uses. For example, the present invention provides the use of any of the above antibodies against Aβ in the manufacture of a medicament or diagnostic composition for use in or for the treatment, prevention or diagnosis of amyloidogenic diseases.

Claims (124)

(I) a variable region complementarity determining region (CDR) from the 12A11 immunoglobulin light chain variable region sequence shown as SEQ ID NO: 2, and (ii) a variable framework region from a human acceptor immunoglobulin light chain sequence. A humanized immunoglobulin light chain. At least one framework residue is replaced with the corresponding amino acid residue from the mouse 12A11 light chain variable region sequence, wherein the framework residue is (a) a residue that directly binds the antigen non-covalently;
(B) a residue adjacent to the CDR;
(I) a 12A11 immunoglobulin light chain variable region sequence shown as SEQ ID NO: 2 selected from the group consisting of residues that interact with CDRs, and (d) residues involved in the VL-VH-interface A humanized immunoglobulin light chain comprising a variable region complementarity determining region (CDR) from and (ii) a variable framework region from a human acceptor immunoglobulin light chain sequence. (I) a variable region complementarity determining region (CDR) from the 12A11 immunoglobulin heavy chain variable region sequence shown as SEQ ID NO: 4, and (ii) a variable framework region from a human acceptor immunoglobulin heavy chain sequence. A humanized immunoglobulin heavy chain. At least one framework residue is replaced with the corresponding amino acid residue from the mouse 12A11 heavy chain variable region sequence, wherein the framework residue is (a) a residue that directly and non-covalently binds the antigen;
(B) a residue adjacent to the CDR;
(I) a 12A11 immunoglobulin heavy chain variable region sequence shown as SEQ ID NO: 4 selected from the group consisting of residues that interact with CDRs, and (d) residues involved in the VL-VH-interface A humanized immunoglobulin heavy chain comprising a variable region complementarity determining region (CDR) from and (ii) a variable framework region from a human acceptor immunoglobulin heavy chain. 3. The light chain of claim 2, wherein residues interacting with the CDRs are identified using as a model a 12A11 light chain based on a solved structure of a murine immunoglobulin light chain that shares at least 70% sequence identity with the 12A11 light chain. chain. The light chain of claim 2, wherein residues interacting with the CDRs are identified using as a model the 12A11 light chain based on the solved structure of a murine immunoglobulin light chain that shares at least 80% sequence identity with the 12A11 light chain. chain. 3. The light chain of claim 2, wherein residues interacting with the CDRs are identified using as a model a 12A11 light chain based on a solved structure of a murine immunoglobulin light chain that shares at least 90% sequence identity with the 12A11 light chain. chain. The heavy chain of claim 4, wherein residues interacting with the CDRs are identified using as a model a 12A11 heavy chain based on a solved structure of a murine immunoglobulin heavy chain that shares at least 70% sequence identity with the 12A11 heavy chain. chain. The heavy chain of claim 4, wherein residues interacting with the CDRs are identified using as a model a 12A11 heavy chain based on a solved structure of a murine immunoglobulin heavy chain that shares at least 80% sequence identity with the 12A11 heavy chain. chain. The heavy chain of claim 4, wherein residues interacting with the CDRs are identified using as a model a 12A11 heavy chain based on a solved structure of a murine immunoglobulin heavy chain that shares at least 90% sequence identity with the 12A11 heavy chain. chain. At least one framework residue is replaced with the corresponding amino acid residue from the mouse 12A11 light chain variable region sequence, wherein the framework residue is a three-dimensional model of the 12A11 immunoglobulin light chain variable region. (I) 12A1 shown as SEQ ID NO: 2, which is a residue capable of affecting light chain variable region conformation or function identified by analysis of
A variable region complementarity determining region (CDR) from one immunoglobulin light chain variable region sequence; and (
ii) A humanized immunoglobulin light chain comprising a variable framework region from a human acceptor immunoglobulin light chain sequence. At least one framework residue is replaced with the corresponding amino acid residue from the mouse 12A11 heavy chain variable region sequence, wherein the framework residue is a three-dimensional model of the 12A11 immunoglobulin heavy chain variable region. (I) 12A1 shown as SEQ ID NO: 4, which is a residue capable of affecting heavy chain variable region conformation or function identified by analysis of
A variable region complementarity determining region (CDR) from one immunoglobulin heavy chain variable region sequence; and (
ii) a humanized immunoglobulin heavy chain comprising a variable framework region from a human acceptor immunoglobulin heavy chain. Residues whose framework residues can interact with the antigen, residues proximal to the antigen binding site, CD
Residues that can interact with R, residues adjacent to CDRs, residues within 6 cm of CDR residues, canonical residues, vernier zone residues, interchain filling residues, rare residues, structural models 12. The light chain of claim 11 selected from the group consisting of glycosylation site residues on the surface of Residues whose framework residues can interact with the antigen, residues proximal to the antigen binding site, CD
Residues that can interact with R, residues adjacent to CDRs, residues within 6 cm of CDR residues, canonical residues, vernier zone residues, interchain filling residues, rare residues, structural models 13. The heavy chain of claim 12 selected from the group consisting of glycosylation site residues on the surface of The framework residues are in light chain positions 2, 4, 36, according to numbering by Kabat
The light of Claim 11 substituted at at least one position selected from the group consisting of 38, 40, 44, 46, 47, 48, 49, 64, 66, 68, 69, 71, 87 and 98. chain. Framework residues are located at heavy chain positions 2, 24, 26 according to Kabat numbering.
27, 28, 29, 37, 39, 45, 47, 48, 67, 71, 73, 78, 91,
13. The heavy chain of claim 12, substituted at at least one position selected from the group consisting of 93, 94 and 103. Framework residues are located at heavy chain positions 24, 28, 2 according to Kabat numbering.
13. The heavy chain of claim 12, substituted at a position selected from the group consisting of 9, 37, 48, 67, 71, 73 and 78. The light chain of claim 11 or 13, wherein framework residues are identified using as a model a 12A11 light chain based on a solved structure of a murine immunoglobulin light chain that shares at least 70% sequence identity with the 12A11 light chain. . 14. The light chain of claim 11 or 13, wherein framework residues are identified using as a model a 12A11 light chain based on a solved structure of a murine immunoglobulin light chain that shares at least 80% sequence identity with the 12A11 light chain. . 14. The light chain of claim 11 or 13, wherein framework residues are identified using as a model a 12A11 light chain based on a solved structure of a murine immunoglobulin light chain that shares at least 90% sequence identity with the 12A11 light chain. . 15. The heavy chain of claim 12 or 14, wherein framework residues are identified using as a model a 12A11 heavy chain based on a solved structure of a murine immunoglobulin heavy chain that shares at least 70% sequence identity with the 12A11 heavy chain. . 15. The heavy chain of claim 12 or 14, wherein framework residues are identified using as a model a 12A11 heavy chain based on a solved structure of a murine immunoglobulin heavy chain that shares at least 80% sequence identity with the 12A11 heavy chain. . The heavy chain of claim 12 or 14, wherein framework residues are identified using as a model a 12A11 heavy chain based on a solved structure of a murine immunoglobulin heavy chain that shares at least 90% sequence identity with the 12A11 heavy chain. . The light chain of any one of claims 1, 2, 5-7, 11, 13, 15 and 18-20, and of claims 3, 4, 8-10, 12, 14, 16-17 and 21-23. A humanized immunoglobulin comprising any one heavy chain, or an antigen-binding fragment of said immunoglobulin. 25. The immunoglobulin or antigen-binding fragment of claim 24, which specifically binds to beta amyloid peptide (Aβ) with a binding affinity of at least 10 ⁻⁷ M. 25. The immunoglobulin or antigen-binding fragment of claim 24, which specifically binds to beta amyloid peptide (Aβ) with a binding affinity of at least 10 ⁻⁸ M. 25. The immunoglobulin or antigen-binding fragment of claim 24, which specifically binds to beta amyloid peptide (Aβ) with a binding affinity of at least 10 ⁻⁹ M. 25. The immunoglobulin or antigen-binding fragment of claim 24, wherein the heavy chain isotype is γ1. 25. The immunoglobulin or antigen-binding fragment of claim 24, which binds to soluble beta amyloid peptide (Aβ). 25. The immunoglobulin or antigen-binding fragment of claim 24 that binds to an aggregating beta amyloid peptide (Aβ). 25. The immunoglobulin or antigen-binding fragment of claim 24 that mediates phagocytosis of beta amyloid peptide (Aβ). 25. The immunoglobulin or antigen-binding fragment of claim 24 that crosses the blood brain barrier within the subject. 25. The immunoglobulin or antigen-binding fragment of claim 24 that reduces beta amyloid peptide (Aβ) plaque burden in a subject. (A) three complementarity determining regions wherein the humanized light chain has an amino acid sequence from the corresponding complementarity determining region of the mouse 12A11 immunoglobulin light chain variable domain shown in SEQ ID NO: 2 (
CDR1, CDR2 and CDR3) and variable region frameworks from human light chain variable region framework sequences, but selected from the group consisting of canonical residues, vernier residues, filling residues and rare residues At least one framework residue is occupied by the same amino acid residue present within the equivalent position of the murine 12A11 immunoglobulin light chain variable region framework; and (b) the humanized heavy chain is SEQ ID NO: Three complementarity determining regions (C) having amino acid sequences from the corresponding complementarity determining regions of the mouse 12A11 immunoglobulin heavy chain variable domain, referred to as 4
DR1, CDR2 and CDR3) and variable region frameworks from human heavy chain variable region framework sequences, but from the second group consisting of canonical residues, vernier residues, filling residues and rare residues At least one framework residue selected is
Occupied by equivalent amino acid residues present within equivalent positions of the mouse 12A11 immunoglobulin heavy chain variable region framework;
Wherein the humanized immunoglobulin is at least 10 in beta amyloid peptide (“Aβ”).
Specifically binds with a binding affinity of ⁻⁷ M, wherein the 12A11 immunoglobulin has a light chain having a variable domain represented by SEQ ID NO: 2 and a heavy chain having a variable domain represented by SEQ ID NO: 4,
A humanized immunoglobulin comprising a humanized heavy chain and a humanized light chain. 35. The humanized immunoglobulin of claim 34, wherein the human light chain variable region framework is derived from a kappa light chain variable region. 35. The humanized immunoglobulin of claim 34, wherein the human heavy chain variable region framework is derived from an IgG1 heavy chain variable region. The light chain variable region framework comprises a light chain sequence of 12A11 immunoglobulin and at least 7
35. The humanized immunoglobulin of claim 34, which is derived from a human immunoglobulin light chain having 0% sequence identity. The heavy chain variable region framework has a heavy chain sequence of 12A11 immunoglobulin and at least 7
35. The humanized immunoglobulin of claim 34, which is derived from a human immunoglobulin heavy chain having 0% sequence identity. Complementarity in which the humanized light chain comprises a complementarity determining region that is identical to the corresponding complementarity determining region of the mouse 12A11 heavy chain and the humanized heavy chain is identical to the corresponding complementarity determining region of the mouse 12A11 heavy chain 35. The humanized immunoglobulin of claim 34, comprising a determining region. The complementarity determining regions of the 12A11 variable light chain sequence shown as SEQ ID NO: 2 (CDR1, CD
A humanized antibody comprising R2 and CDR3). The complementarity determining region (CDR1, CD) of the 12A11 variable heavy chain sequence shown as SEQ ID NO: 4
A humanized antibody comprising R2 and CDR3). A humanized antibody that specifically binds to beta amyloid peptide (Aβ), or an antigen-binding fragment thereof, comprising a variable region comprising a complementarity determining region (CDR) corresponding to a CDR from mouse 12A11 antibody. Having the variable light chain amino acid sequence shown as SEQ ID NO: 6 and SEQ ID NO: 8, SEQ ID NO: 9
And a humanized immunoglobulin having a variable heavy chain amino acid sequence selected from the group consisting of SEQ ID NO: 10. A chimeric immunoglobulin comprising a variable region sequence essentially as shown in SEQ ID NO: 2 or SEQ ID NO: 4, and a constant region sequence from a human immunoglobulin. 44. A method for preventing or treating amyloid disease in a patient, comprising administering to the patient an effective dose of the humanized immunoglobulin of any one of claims 24-43. 44. A method for preventing or treating Alzheimer's disease in a patient, comprising administering to the patient an effective dose of the humanized immunoglobulin of any one of claims 24-43. 48. The method of claim 46, wherein the effective dose of humanized immunoglobulin is at least about 1 mg / kg body weight. 48. The method of claim 46, wherein the effective dose of humanized immunoglobulin is at least about 10 mg / kg body weight. 44. A pharmaceutical composition comprising the immunoglobulin of any one of claims 24-43 and a pharmaceutical carrier. Amino acids 43-58 of SEQ ID NO: 2, amino acids 74-80 of SEQ ID NO: 2 and SEQ ID NO: 2
An isolated polypeptide comprising a fragment of SEQ ID NO: 2 selected from the group consisting of amino acids 113 to 121 of Amino acids 43-58 of SEQ ID NO: 2, amino acids 74-80 of SEQ ID NO: 2 and SEQ ID NO: 2
An isolated polypeptide comprising the amino acids 113-121 of Amino acids 50-56 of SEQ ID NO: 4, amino acids 71-86 of SEQ ID NO: 4, and SEQ ID NO: 4
An isolated polypeptide comprising a fragment of SEQ ID NO: 4, selected from the group consisting of amino acids 119 to 128 of: Amino acids 50-56 of SEQ ID NO: 4, amino acids 71-86 of SEQ ID NO: 4, and SEQ ID NO: 4
An isolated polypeptide comprising amino acids 119 to 128 of   An isolated polypeptide comprising amino acids 1-131 of SEQ ID NO: 2.   An isolated polypeptide comprising amino acids 1 to 139 of SEQ ID NO: 4. An isolated polypeptide having at least 85% identity to amino acids 1-131 of SEQ ID NO: 2. An isolated polypeptide having at least 85% identity to amino acids 1 to 139 of SEQ ID NO: 4. 58. The isolated polypeptide of claim 56 or 57, wherein the polypeptides have at least 86% identity. 58. The isolated polypeptide of claim 56 or 57, wherein the polypeptides have at least 87% identity. 58. The isolated polypeptide of claim 56 or 57, wherein the polypeptides have at least 88% identity. 58. The isolated polypeptide of claim 56 or 57, wherein the polypeptides have at least 89% identity. 58. The isolated polypeptide of claim 56 or 57, wherein the polypeptides have at least 90% identity. 58. The isolated polypeptide of claim 56 or 57, wherein the polypeptide has at least 90% or greater identity. The variant is a beta amyloid peptide (Aβ) with a binding affinity of at least 10 ⁷ M ⁻¹
A variant of the polypeptide comprising the amino acid sequence of SEQ ID NO: 2, wherein the variant retains the ability to specifically bind to wherein the variant comprises at least one conservative amino acid substitution. The variant is a beta amyloid peptide (Aβ) with a binding affinity of at least 10 ⁷ M ⁻¹
A variant of the polypeptide comprising the amino acid sequence of SEQ ID NO: 4, wherein the variant comprises at least one conservative amino acid substitution, retaining the ability to specify specific binding to. 21. An isolated nucleic acid molecule encoding the light chain of any one of claims 1, 2, 5-7, 11, 13, 15 and 18-20. 24. An isolated nucleic acid molecule encoding the heavy chain of any one of claims 3, 4, 8-10, 12, 14, 16-17 and 21-23. 66. An isolated nucleic acid molecule encoding the polypeptide of any one of claims 46-65.   45. An isolated nucleic acid molecule encoding the immunoglobulin of any one of claims 21-44.   An isolated nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO: 1 or 3.   A vector comprising the nucleic acid molecule of any of claims 66-70.   A host cell comprising the nucleic acid molecule of any of claims 66-70. A transgenic animal expressing a polypeptide encoded by the nucleic acid molecule of any of the preceding claims.   74. The transgenic animal of claim 73, wherein the polypeptide is expressed in the milk of the animal. 75. A method of producing an antibody, or fragment thereof, comprising culturing the host cell of claim 72 under conditions such that the antibody or fragment is produced and isolating said antibody from the host cell or culture. Amino acids 43-58 of SEQ ID NO: 2, amino acids 74-80 of SEQ ID NO: 2 and SEQ ID NO: 2
A method of producing an antibody or antibody fragment comprising a fragment of SEQ ID NO: 2 selected from the group consisting of amino acids 113-121 of: a host cell comprising a nucleic acid molecule encoding said antibody or antibody fragment. The method as described above, comprising culturing under conditions such that the antibody or antibody fragment is produced and isolating the antibody or antibody fragment from the host cell or culture. Amino acids 50-56 of SEQ ID NO: 4, amino acids 71-86 of SEQ ID NO: 4, and SEQ ID NO: 4
A method of producing an antibody or antibody fragment comprising a fragment of SEQ ID NO: 4 selected from the group consisting of amino acids 119-128 of a host cell comprising a nucleic acid molecule encoding the antibody or antibody fragment. The method as described above, comprising culturing under conditions such that the antibody or antibody fragment is produced and isolating the antibody or antibody fragment from the host cell or culture. Model the three-dimensional structure of the 12A11 variable region based on the elucidated immunoglobulin structure, and the model for residues that can affect 12A11 immunoglobulin variable region conformation or function so that residues that are susceptible to substitution are identified. A method for identifying residues that are susceptible to substitution within the humanized 12A11 immunoglobulin variable framework region. Use of a variable region sequence shown as SEQ ID NO: 2 or SEQ ID NO: 4, or any part thereof in the creation of a three-dimensional image of a 12A11 immunoglobulin, 12A11 immunoglobulin chain, or domain thereof. A method of imaging amyloid deposits in a patient's brain, comprising administering to the patient an agent that specifically binds Aβ and detecting an antibody bound to Aβ. 81. The method of claim 80, wherein the agent is an antibody comprising the light chain variable sequence set forth in SEQ ID NO: 2 and the heavy chain variable region sequence set forth in SEQ ID NO: 4, or an antigen-binding fragment of the antibody.   The antigen binding fragment is a Fab fragment. 81. The method of claim 80. 83. A kit for imaging by the method of any one of claims 80-82, comprising instructions for use. A nucleic acid molecule encoding an immunoglobulin light chain comprising a CDR of the amino acid sequence of SEQ ID NO: 2 and a nucleic acid molecule encoding an immunoglobulin heavy chain comprising the CDR of the amino acid sequence of SEQ ID NO: 4 A method of treating amyloid disease comprising administering to a patient with amyloid disease such that a beneficial therapeutic response occurs within the patient under conditions where is expressed. A nucleic acid molecule encoding an immunoglobulin light chain comprising the CDR of the amino acid sequence of SEQ ID NO: 7 and a nucleic acid molecule encoding an immunoglobulin heavy chain comprising the CDR of the amino acid sequence of SEQ ID NO: 10 or SEQ ID NOs: 13-31 Administering to a patient with amyloid disease such that a beneficial therapeutic response occurs within the patient under conditions in which the immunoglobulin is expressed. A method of treating a disease. A method of preventing or treating a disease associated with amyloid deposition of Aβ in a patient's brain, comprising administering to the patient an effective dose of the humanized immunoglobulin of any of the preceding claims.   87. The method of claim 86, wherein the disease is cognitive impairment.   90. The method of claim 86, wherein the disease is Alzheimer's disease.   90. The method of claim 86, wherein the disease is Down syndrome.   90. The method of claim 86, wherein the disease is mild cognitive impairment.   90. The method of claim 86, wherein the antibody is human isotype IgG1.   The method of any of the preceding claims, wherein the disease is a human. 94. The method of any one of claims 86 to 92, wherein after administration, the antibody binds to amyloid deposits in the patient and induces a clearance response to amyloid deposits.   94. The method of claim 93, wherein the ablation reaction is an Fc receptor mediated phagocytosis reaction.   95. The method of claim 93 or 94, further comprising monitoring the removal reaction. 93. The method of any one of claims 86 to 92, wherein the antibody binds to the patient's soluble A [beta] after administration. 93. The method of any one of claims 86 to 92, wherein the antibody binds to soluble A [beta] in serum, blood or cerebrospinal fluid within the patient after administration.   98. The method of any one of claims 86 to 97, wherein the patient is asymptomatic.   99. The method of any one of claims 86-98, wherein the patient is less than 50 years old. 99. The patient has a genetic risk factor indicative of susceptibility to Alzheimer's disease.
The method according to any one of the above. 101. The dose of antibody is at least 1 mg per kg patient body weight.
The method according to any one of the above. 11. The dose of antibody is at least 10 mg / kg patient body weight.
The method according to any one of 0. 103. The method of any one of claims 86-102, wherein the antibody is administered with a carrier as a pharmaceutical composition. 8. The antibody is administered intraperitoneally, orally, intranasally, subcutaneously, intramuscularly, topically or intravenously.
The method of any one of 7-103. 15. A method of reducing plaque burden in a subject in need thereof comprising administering to the subject an effective dose of the humanized immunoglobulin of any one of the preceding claims. 106. The method of claim 105, wherein after administration, the antibody binds to amyloid deposits in the patient and induces elimination of response to amyloid deposits.   107. The method of claim 106, wherein the elimination reaction is an Fc receptor mediated phagocytosis reaction.   108. The method of claim 106 or 107, further comprising monitoring the removal reaction.   106. The method of claim 105, wherein the antibody binds to soluble Aβ in the patient after administration. 106. The method of claim 105, wherein the antibody binds to soluble Aβ in the patient's serum, blood or cerebrospinal fluid after administration. Any one of the preceding claims comprising an Fc region with altered effector function
The term humanized immunoglobulin. Amino acid 3 to beta amyloid peptide (Aβ), wherein the antibody is an IgG1 isotype
8. A method of reducing a neuritic burden in a subject in need thereof comprising administering to the subject an effective dose of a humanized 12A11 antibody that binds to an epitope within 7.   113. The method of claim 112, wherein the subject has amyloid disease.   114. The method of claim 113, wherein the amyloid disease is Alzheimer's disease. Administer an effective dose of a humanized 12A11 antibody that binds to an epitope within amino acids 3-7 of Aβ and is an IgG1 isotype capable of reducing beta amyloid peptide (Aβ) load and neuritic dystrophy in a patient A method of treating amyloid disease in a patient comprising.   116. The method of claim 115, wherein the amyloid disease is Alzheimer's disease. Administering to a mammal an effective dose of a humanized 12A11 antibody that binds to an epitope within amino acids 3-7 of Aβ and is an IgG1 isotype, capable of reducing beta amyloid peptide (Aβ) load and neuritic dystrophy A method of reducing beta amyloid peptide (Aβ) load and neuritic dystrophy in a mammal comprising.   118. The method of claim 117, wherein the mammal is a human.   119. The method of claim 117 or 118, wherein the mammal has amyloid disease.   120. The method of claim 119, wherein the amyloid disease is Alzheimer's disease. A therapeutic composition comprising a humanized 12A11 antibody of the IgG1 isotype that binds to an epitope within amino acids 3-7 of beta amyloid peptide (Aβ), wherein the antibody can reduce the neuritic burden in the subject. An effective dose of a humanized 12A11 antibody that binds to an epitope within amino acids 3-7 of Aβ and binds to soluble beta amyloid peptide (Aβ), which is an IgG1 isotype, and reduces neuritic dystrophy in a patient. Comprising administering to a patient,
A method of treating amyloid disease in a patient.   123. The method of claim 122, wherein the amyloid disease is Alzheimer's disease. A therapeutic composition comprising a humanized 12A11 antibody of the IgG1 isotype that binds to soluble beta amyloid peptide (Aβ), wherein the antibody binds to an epitope within amino acids 3-7 of Aβ and can reduce the neuritic burden in a patient object.

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