JP2008032695A - Panel for analysis and analysis device using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a panel for analysis capable of preventing contamination and a shortage of sample solution even if the sample solution is attached to a periphery of an inlet. <P>SOLUTION: A chamber with which sample solution spotted to an inlet 14 is transferred is placed inside a panel 3 for analysis. The inlet 14 is formed protruding in a direction separating away from the chamber. A recess 12 is formed around the inlet 14. The inlet 14 is positioned on a panel holding member 101 for analysis of an analysis device on a center of rotation axis side so as to optically access and analyze the chamber by rotating the panel holding member 101 for analysis. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an analysis panel and an analysis apparatus for measuring a reaction state between a sample solution and an analysis reagent, and more specifically, a note on an analysis panel used for measuring a component of a sample solution in the analysis apparatus. The present invention relates to an inlet structure and a means for transferring sample liquid adhering to the vicinity of the inlet.

  Conventionally, an analysis panel in which a sample solution is set inside is used, and an analysis disk to which the analysis panel is attached is rotated around an axis while analyzing the characteristics of the sample solution using an optical scanning technique. The device has been put into practical use.

  In recent years, there have been many demands from the market, such as sample volume reduction, device miniaturization, short time measurement, multi-item simultaneous measurement, etc., reacting sample liquids such as blood with various analytical reagents, detecting the mixture, There is a demand for a more accurate analyzer that can inspect the progress of various diseases in a short time.

For example, Patent Document 1 describes a configuration shown in FIGS. 20 (a) and 20 (b).
As shown in FIG. 20B, the analysis panel 203 is mounted on the analysis panel holding unit 204 of the analysis disk 201, and the analysis disk 201 shown in FIG. The sample liquid is optically analyzed by rotating around the axis 202.

  The analysis panel 203 detachably attached to the analysis disk 201 includes a sample solution injection port 214, a cavity 208 communicating with the injection port 214, and an air port 210 communicating with the cavity 208. In addition to facilitating the injection of the sample solution by being formed on the end face of the panel for analysis 203, an absorption member 215 is formed on the analysis disk 201 corresponding to the position of the inlet 214 of the panel for analysis 203, The absorbing member 215 absorbs an appropriate amount of the sample liquid attached around the inlet 214 of the analysis panel 203. When the analysis panel 203 is attached to the analysis panel holding unit 204, the inlet 214 of the analysis panel 203 is sealed with the absorbing member 215.

  A channel is formed in the cavity 208 of the analysis panel 203 so as to be positioned on the outer periphery of the analysis disk 201 with respect to the inlet 214 and the air port 210. An analysis reagent 209 for reacting is applied.

  In the analysis operation using the analysis panel 203, when the sample liquid is spotted on the inlet 214 of the analysis panel 203 in a state where the analysis panel 203 is detached from the analysis disk 201, the sample liquid is separated from the inlet 214. It is transferred by capillary force into the communicating cavity 208.

When the analysis panel 203 on which the sample solution is set is attached to the analysis panel holding unit 204 of the analysis disk 201, the opening of the inlet 214 is closed by the analysis disk 201. At this time, the sample liquid adhering to the end face of the injection port 214 is absorbed by coming into contact with the absorbing member 215, so that the sample liquid adheres to the position facing the injection port 214 and the analysis disk 201 is rotating. The sample liquid can be prevented from scattering and the subsequent analysis of the sample liquid can be performed safely.
JP 2003-185671 A

  However, when the sample liquid adhering to the periphery of the inlet 214 is sucked by the absorption member 215, the sample liquid injected into the cavity 208 of the analysis panel 203 is not sucked by the absorption member 215, and the analysis reagent 209 The sample solution required at the time of mixing is insufficient, which has a problem of affecting the measurement of the reaction state between the analytical reagent 209 and the sample solution.

  In addition, since the absorbing member 215 is provided on the analysis disk 201 side, when the analysis disk 201 is repeatedly used, the analysis panel 203 is mounted on the analysis disk 201 and analyzed. Since the sample liquid adhering to the periphery of the inlet 214 is sucked by the absorption member 215, the suction member 215 is gradually contaminated with the sample liquid. This causes a possibility that a contaminated substance is mixed into the sample liquid to be measured to adversely affect the measurement, or that the operator touches the contaminated absorbing member 215 to infect a disease or the like. . Further, every time analysis is performed, laborious work such as replacement of the absorbing member 215 with a new one or cleaning is caused, and there is a difficult problem in terms of safety management.

  Therefore, the present invention solves the above-mentioned problems, and prevents the occurrence of a situation that affects measurement, such as lack of sample liquid and prevention of contamination, even when the sample liquid adheres around the inlet 214. An analysis panel that can be used and an analysis apparatus using the same are provided.

  The analysis panel according to claim 1 of the present invention is a sample in which an inlet for a sample liquid is provided on one side surface of the panel body, and the sample body is spotted on the inlet in communication with the inlet in the panel body. An analysis panel for analyzing a component in the sample liquid by rotating the panel body in a state in which a chamber for transferring the liquid is provided and the injection port is disposed on the rotation axis side; The inlet has a shape protruding from the one side of the panel body in a direction away from the chamber, and a recess is formed around the inlet before the one side of the panel body.

The analysis panel according to claim 2 of the present invention is characterized in that, in claim 1, the amount of protrusion of the inlet is substantially equal to one side surface of the panel main body.
The analysis panel according to claim 3 of the present invention is characterized in that, in claim 1, the cross-sectional area of the opening of the concave portion is equal to or larger than the cross-sectional area at the back end of the concave portion.

The analysis panel according to claim 4 of the present invention is characterized in that, in claim 1, the injection port protrudes with the bottom surface of the recess as a base end.
The analysis panel according to claim 5 of the present invention is the analysis panel according to claim 1, wherein the volume of the recess is large enough to accept the sample liquid adhering to the vicinity of the inlet when the sample liquid is spotted on the inlet. It is characterized by being.

The analysis panel according to claim 6 of the present invention is characterized in that, in claim 1, an absorbing member that absorbs the sample solution is disposed in the recess.
The analysis panel according to claim 7 of the present invention is the analysis panel according to claim 6, wherein the absorbing member is arranged at a position in contact with the sample liquid transferred to the recess by the centrifugal force generated by the rotation around the axis. It is characterized by.
The analysis panel according to an eighth aspect of the present invention is characterized in that, in the first aspect, a groove portion is formed which communicates with the concave portion and holds the sample liquid by a capillary force.

The analysis panel according to claim 9 of the present invention is characterized in that, in claim 8, the groove portion communicates with a bottom portion of the recess.
The analysis panel according to claim 10 of the present invention is the analysis panel according to claim 8, wherein the groove portion has a sample liquid transferred to the concave portion due to a centrifugal force generated by the rotation around the axis, and the centrifugal force further includes an internal portion of the sample liquid. It is the shape conveyed by this.

The analysis panel according to claim 11 of the present invention is characterized in that, in claim 1, the recess communicates with the chamber.
According to a twelfth aspect of the present invention, in the analysis panel according to the twelfth aspect, the sample liquid adhering to the vicinity of the inlet through the passage communicating the concave portion and the chamber is generated by rotation around the axis. It is configured to be transferred into the chamber by centrifugal force.

  The analysis panel according to claim 13 of the present invention is characterized in that, in claim 1, the chamber communicated with the inlet has an analysis reagent used for analyzing blood as a sample solution.

The analysis panel according to a fourteenth aspect of the present invention is characterized in that in the first aspect, an openable / closable cover for covering the inlet and the recess is provided.
The analysis panel according to claim 15 of the present invention is the analysis panel according to claim 1, further comprising an openable / closable cover that covers the inlet and the recess, and an absorption member that absorbs the sample liquid is provided inside the cover. Features.

  The analysis panel according to a sixteenth aspect of the present invention is the analysis panel according to the first aspect, further comprising an openable / closable cover that covers the inlet and the recess, and an absorption member that absorbs the sample liquid is provided inside the cover. A gap is formed between the inlet and the absorbing member.

  The analysis panel according to a seventeenth aspect of the present invention is the analysis panel according to the first aspect, wherein the chamber holds a holding chamber for temporarily holding the sample liquid spotted on the inlet and an analysis reagent necessary for the analysis. And a measurement chamber region in which the sample liquid held in the holding chamber and the analysis reagent are transferred, mixed with each other, and measurement of the sample liquid mixed with the analysis reagent is performed. And

  The analysis panel according to an eighteenth aspect of the present invention is the analysis panel according to any one of the first, twelfth, and fourteenth aspects, wherein the peripheral surface of the inlet, the concave portion, the concave portion and the chamber communicate with each other. A surfactant is applied to at least one of the passage and the inner surface of the cover member.

  According to a nineteenth aspect of the present invention, there is provided an analysis chamber according to the present invention, wherein a chamber is provided in the chamber to communicate with a sample liquid injection port provided on one side of the panel body and to transfer a sample liquid spotted on the injection port. An analysis panel, an analysis panel holding member on which the analysis panel is mounted, and a sample liquid spotted on the inlet by the centrifugal force generated by rotating the analysis panel holding member. And an analytical device for optically accessing the sample liquid in the chamber to detect and analyze a signal, wherein the analytical panel is provided with an openable / closable cover for covering the inlet. The analysis panel in a closed state is configured to perform an analysis operation by being attached to the analysis panel holding member so that the inlet crosses the rotational axis of the analysis panel holding member.

The analyzer according to claim 20 of the present invention is characterized in that, in claim 19, an absorption member for absorbing the sample liquid is provided inside the cover.
According to a twenty-first aspect of the present invention, there is provided the analyzer according to the nineteenth aspect, wherein a recess for collecting the sample droplet adhering to the vicinity of the inlet of the analysis panel is provided inside the cover. Features.

According to a twenty-second aspect of the present invention, in the twenty-first aspect, a groove for holding a sample solution by a capillary force is formed in the concave portion.
According to a twenty-third aspect of the present invention, in the nineteenth aspect, a surfactant is applied to at least one of the peripheral surface of the inlet of the analysis panel and the inner surface of the cover member. It is characterized by that.

  According to a twenty-fourth aspect of the present invention, there is provided an analysis panel according to any one of the first to eighth aspects, an analysis panel holding member to which the analysis panel is mounted, and the analysis panel holding. An analyzer for transferring a sample liquid spotted on the inlet by a centrifugal force generated by rotating a member to the chamber and optically accessing the sample liquid in the chamber to detect and analyze the signal The analysis panel may be arranged such that the injection port is closer to the outer peripheral side of the analysis panel holding member than the rotation axis, or the injection port crosses the rotation axis of the analysis panel holding member. The analysis panel is mounted on the analysis panel holding member to perform an analysis operation.

  In the analysis panel according to claim 25 of the present invention, a sample liquid inlet is provided on one side surface of the panel body, and the sample communicated with the inlet inside the panel body and spotted on the inlet. A chamber through which the liquid is transferred, the panel body is rotated about an axis, and an analysis panel for analyzing the components in the sample liquid in the chamber is provided. The sample liquid has a shape protruding in a direction away from the chamber from the one side surface, and an openable / closable cover for covering the injection port is provided on the panel body, and is attached to the inside of the cover in the vicinity of the injection port. A recess for collecting droplets or an absorption member for absorbing the sample droplet is provided, and when the cover is closed, between the injection port and the recess, or between the injection port and the absorption member. In the sky Characterized in that the formation of the.

  In the analysis panel of the present invention, the injection port provided on one side surface of the panel main body has a shape protruding in a direction away from the chamber from the one side surface of the panel main body, and the injection is performed in front of the one side surface of the panel main body. Since a concave portion is formed around the inlet, the centrifugal force is generated when the panel body is rotated with the inlet arranged on the rotation axis side and the components in the sample solution are analyzed in the chamber. When this occurs, the sample liquid adhering to the periphery of the injection port is reliably transferred to the recess and collected, and the effect of preventing the sample liquid injected into the chamber from being discharged in the reverse direction outside the chamber is obtained. .

  Further, the analyzer of the present invention is provided for analysis in which a chamber is provided in which a sample liquid spotted at the inlet is transferred to and communicated with an inlet of a sample liquid provided on one side of the panel body. A panel, an analysis panel holding member on which the analysis panel is mounted, a sample solution spotted on the inlet by centrifugal force generated by rotating the analysis panel holding member, An analyzer for optically accessing a sample liquid in a chamber to detect and analyze a signal, wherein an openable / closable cover for covering the inlet is provided on the analysis panel, and the cover is closed The analysis panel in the state is configured to perform an analysis operation by being attached to the analysis panel holding member so that the inlet crosses the rotational axis of the analysis panel holding member. When wood is centrifugal force to resettlement, sample liquid is spotted on the inlet is transported toward the chamber. In addition, the sample liquid adhering to the periphery of the injection port at the time of spotting moves in the opposite direction to the chamber and is surely collected in the cover to prevent it from being scattered outside and contaminated. The effect that can be obtained.

Hereinafter, embodiments of the present invention will be described with reference to FIGS.
(Embodiment 1)
1 to 6 show Embodiment 1 of the present invention.

FIG. 1 shows an analysis panel 3 of the present invention, and FIG. 2 shows an exploded view thereof.
The analysis panel 3 is formed by bonding the upper substrate 1 and the lower substrate 2, and one surface 15 forming an injection port 14 is formed on one surface of the lower substrate 2 and injected into the injection port 14. Holding chamber 4 for holding the sample solution, reagent chamber 5 for holding an analysis reagent (not shown), sample solution and analysis reagent held in the holding chamber 5 are transferred and mixed together In addition, a measurement chamber region 7 in which measurement of the sample liquid mixed with the analysis reagent is performed, a channel 6 that communicates the reagent chamber 5 and the measurement chamber region 7, and a channel that communicates the measurement chamber region 7 to the air opening hole 9. 8 are formed.

  In this embodiment, the chamber in which the sample liquid and the analysis reagent are mixed and the chamber in which the measurement of the sample liquid mixed with the analysis reagent is configured as the integrated measurement chamber region 7. And a chamber in which the analysis reagent is mixed, and a chamber in which measurement of the sample liquid mixed with the analysis reagent is performed.

  The upper substrate 1 is bonded to the lower substrate 2, the opening surfaces of the holding chamber 4, the reagent chamber 5, the measurement chamber region 7, the flow path 6 and the flow path 8 are closed, and a cavity having a predetermined size gap is formed. Each function functions such as transfer of a sample solution by capillary force and holding a predetermined amount of solution. The inlet 14 is formed by joining the one side 15 of the lower substrate 2 and the one side 16 of the upper substrate 1.

  FIG. 3 shows a state in which the analysis panel 3 is mounted on the analysis panel holding member 101 of the analyzer. The analyzer optically analyzes the characteristics of the sample solution while rotating the disc-shaped analysis panel holding member 101 around the axis 11 by the rotation driving means.

  As shown in FIG. 4, the inlet 14 of the analysis panel 3 is formed so as to protrude in a direction away from the holding chamber 4. That is, the inlet 14 of the analysis panel 3 in a state where the analysis panel 3 is set on the analysis panel holding member 101 has a shape protruding in a direction approaching the axis 11 from one side surface of the main body of the analysis panel 3. It is formed and it is easy to supply the sample solution. Specifically, when blood from a human body is collected and set as a sample solution, a puncture needle such as a lancet as a blood collection puncture aid is pressed against a blood collection site such as a fingertip, and blood is collected. The sample liquid is injected into the holding chamber 4 by capillary force or the like by allowing the injection port 14 to come into contact with a site to be applied, and the sample liquid can be easily supplied. It is possible to prevent blood from adhering to the position.

FIG. 5 shows the configuration of the analyzer.
This analysis apparatus includes an analysis panel holding member 101 to which the analysis panel 3 is mounted, a motor 102 as a rotation driving means for rotating the analysis panel holding member 101 around the axis 11, and the analysis panel 3. Optical measuring means 104 for optically measuring the solution in the inside, control means 105 for controlling the rotational speed and direction of the analysis panel holding member 101, the measurement timing of the optical measuring means 104, and the like, and optical measuring means When the signal obtained by 104 is processed and the sample liquid is blood, in addition to the concentration and amount of the specific substance in the blood, the calculation unit 106 calculates the shape and size of the substance according to the purpose of analysis. And a display unit 107 for displaying the result obtained by the calculation unit 106.

  The optical measurement means 104 includes a laser light source 103 for irradiating the measurement unit of the analysis panel 3 with laser light, and the amount of transmitted light that has passed through the analysis device 1 out of the laser light emitted from the laser light source 103. And a laser light source 103 and a photodetector 108 corresponding to the type of wavelength required for the measurement can be provided.

  Depending on the application, this analyzer uses the centrifugal force generated by the rotation around the axial center depending on the configuration of the chamber and flow path in the analysis panel 3 to transfer the liquid in the panel or perform centrifugation. It can also be a centrifuge. The shape of the analysis panel may be a fan shape, a cube shape, or other shapes, and a plurality of these analysis panels 3 may be mounted on the analysis panel holding member 101 simultaneously.

  As shown in FIG. 4 showing the enlargement of the peripheral portion of the inlet 14, only the side of the axis 11 is opened around the inlet 14 on one side of the analysis panel 3, and the outer peripheral direction from the axis 11. A concave portion 12 that is recessed toward is formed. The recess 12 is formed in a gently curved shape so that the cross-sectional area of the opening on the side of the axis 11 is equal to or larger than the cross-sectional area of the opening on the outer peripheral side of the recess 12. When the centrifugal force is generated in the state shown in FIG. 4, the sample liquid adhering to the periphery of the inlet 14 is surely transferred to the concave portion 12 and further easily transferred to the lowest position of the concave portion 12 and out of the concave portion 12. Can be collected without splashing.

  In addition, the projections of the injection port 14 are formed by the one surfaces 15 and 16 so as to protrude in the direction approaching the axis 11 from the bottom surface of the opened recess 12, so that they adhere to the periphery of the injection port 14. Although the sample liquid is transferred into the recess 12, the transferred position is almost the bottom surface in the recess 12, so that the sample liquid can be stably collected without overflowing from the recess 12, The effect that it can collect with one recessed part 12 is also acquired.

  In other words, the sample liquid adhering to the vicinity of the injection port 14 is transferred into the recess 12 through the surface of the protruding portion forming the injection port 14 by the centrifugal force generated by the rotation around the axis. is there. At this time, the sample solution in the holding chamber 4 is transferred into the reagent chamber 5 in which the analysis reagent is previously supported by centrifugal force simultaneously with the transfer of the sample solution adhering to the vicinity of the inlet 14 into the recess 12. Be transported. The liquid sample that has flowed into the reagent chamber 5 is mixed with the analysis reagent carried in the reagent chamber 5 by swinging due to the rotation acceleration of the analysis panel holding member 101 or diffusion of the liquid while the rotation is stopped. It is also possible to mix by applying an external force that directly vibrates the reagent chamber 5 itself.

  When the mixing of the analysis reagent and the sample solution reaches a predetermined level, the sample solution in the reagent chamber 5 is transferred to the entrance of the measurement chamber region 7 through the channel 6 by capillary force. The laser beam irradiated from the laser light source 103 passes through the measurement chamber region 7 and the concentration of the component can be measured by measuring the absorbance of the reaction state between the sample liquid and the analysis reagent with the photodetector 108.

  The volume of the recess 12 is set to a size that can accept the sample solution adhering to the vicinity of the injection port 14 when the sample solution is spotted on the injection port 14, so that the sample solution overflows from the recess 12. This has the effect of preventing the volume from being transferred. Assuming that blood is applied as the sample liquid, in the case of spotting by fingertip blood collection using a puncture device such as a lancet, the amount of the sample liquid is estimated to be about 10 μl, and this spotting quantity Since the amount of blood collected in a range not exceeding 1 is generally injected from the inlet 14, the volume of the recess 12 is set to 10 μl at the maximum.

Furthermore, as shown in FIG. 6, by providing the analysis panel 3 with an openable / closable cover 18 that covers the inlet 14 and the recess 12, the following effects can be obtained.
The cover 18 is opened, the sample solution is spotted on the inlet 14, and then the analysis panel 3 is mounted on the analysis panel holding member 101 in a state where the cover 18 is closed. Even if the sample liquid or the sample liquid transferred into the chamber 4 flows out to the side surface of the analysis panel 3 near the inlet 14 and the recess 12 due to some influence, it is received by the cover 18 and released to the outside. Occurrence can be prevented. In addition, after the analysis, by disposing the cover 18 without opening it, the occurrence of contamination can be prevented, which is suitable for a disposable analysis panel.

  Further, when a surfactant is applied to the peripheral surface of the inlet 14, the sample liquid is transferred to the peripheral surface of the inlet 14 when the sample liquid is transferred to the recess 12 by the centrifugal force. By the hydrophilic treatment, it can be smoothly transferred.

  As described above, since the concave portion 12 is formed around the inlet 14, the sample liquid adhering to the vicinity of the inlet 14 can be analyzed without being scattered and contaminated, and further injected into the chamber 4. An effect of preventing the sample liquid that has been discharged from being discharged in the reverse direction outside the chamber can be obtained.

(Embodiment 2)
7 and 8 show a second embodiment of the present invention.
The main configuration of the analysis panel 3, the analysis panel holding member 101 to which the analysis panel 3 is mounted, and the method for measuring the reaction state between the sample solution and the analysis reagent are the same as those described in the first embodiment. Since this is the same, the description here is omitted.

FIG. 7 shows a configuration in the vicinity of the inlet 14 of the analysis panel 3 in the second embodiment.
The tip of the inlet 14 is located at a position substantially equivalent to one side surface of the main body of the analysis panel 3, and only the surface on the axis 11 side opens around the inlet 14. A recess 12 that is recessed toward the outer peripheral direction is formed. Further, the recess 12 is formed so that the cross-sectional area of the opening on the axis 11 side of the recess 12 is equal to or larger than the cross-sectional area of the opening on the outer peripheral side of the recess 12. The inlet 14 is formed so as to protrude from the bottom surface of the recess 12, and the volume of the recess 12 is large enough to accept a sample droplet adhering to the vicinity of the inlet when the sample liquid is spotted on the inlet 14. It consists of Here, the amount that can be spotted by one fingertip blood collection using a puncture device such as a lancet is limited to about 10 μl, and it is common to inject a blood collection volume within a range not exceeding the amount of spotting from the injection port 14. Is. Based on this idea, the capacity of the recess 12 is set to 10 μl at the maximum in the present invention.

Further, the recess 12 is provided with an absorbing member 22 such as a nonwoven fabric made of polypropylene or paper material as a material that absorbs the sample liquid.
With this configuration, when the sample liquid is spotted on the inlet 14 and the sample liquid is supplied, the sample droplet 19 is attached in the vicinity of the inlet 14 as shown in FIG. . The analysis panel 3 to which the sample droplet 19 is attached is attached to the analysis panel holding member 101 as it is, and the sample droplet adhering to the vicinity of the inlet 14 is recessed by the centrifugal force generated by rotating around the axis 11. 12 is moved in the direction of the arrow 20, transferred to the absorption member 22, and finally sucked by the absorption member 22.

  In the state where the absorbing member 22 that absorbs the sample liquid is disposed, the sample liquid adhering to the vicinity of the inlet is transferred to the recess 12 by directly using the action of a predetermined centrifugal force used for transferring the sample liquid inside the panel body. Furthermore, the absorption member 22 provided on the outer peripheral side of the injection port can absorb and collect the sample liquid adhering to the vicinity of the injection port that has been transferred. There is an effect that the effect of collecting can be increased.

  After injecting a predetermined amount of the sample liquid into the analysis panel 3, it is attached to the analysis panel holding member 101, and the number of rotations necessary for transferring the sample droplet is 1000 rpm or more. When hydrophilic treatment is performed on the entire surface and the vicinity of the injection port 14 with a surfactant or the like, the sample droplet can be transferred only by applying a centrifugal force by rotation of several hundred rpm.

Further, the absorbing member 22 may be provided on the bottom surface of the recess 12, or may be provided between the inlet 14 and the bottom surface of the recess 12.
Further, by providing the absorbing member 22, the sample droplet 19 once held on the absorbing member 22 leaks out of the absorbing member 22 even if the absorbing member 22 is tilted in the direction in which gravity acts after the analysis is completed. There is an effect that can be prevented.

  Further, as in the case of the first embodiment, the cover 18 is provided on the analysis panel 3, the cover 18 is opened, the sample liquid is spotted on the inlet 14, and then the analysis panel is closed. By mounting 3 on the analysis panel holding member 101, the reliability of preventing the sample liquid from flowing out is further improved.

(Embodiment 3)
9 and 10 show Embodiment 3 of the present invention.
In the second embodiment, the absorbing member 22 is provided between the bottom surface in the concave portion 12 or between the inlet 14 and the bottom surface in the concave portion 12, but in the third embodiment, the groove portion 17 communicating with the concave portion 12 is provided, and the capillary tube is provided. The only difference is that the sample droplet 19 is held by force, and the rest is the same as in the second embodiment.

FIG. 9 shows the vicinity of the inlet 14 of the analysis panel 3 in the third embodiment.
The tip of the inlet 14 is located at a position substantially equivalent to one side surface of the main body of the analysis panel 3, and only the surface on the axis 11 side opens around the inlet 14. A recess 12 that is recessed toward the outer circumferential direction is formed. Further, the recess 12 is formed so that the cross-sectional area of the opening on the axis 11 side of the recess 12 is equal to or larger than the cross-sectional area of the opening on the outer peripheral side of the recess 12. The inlet 14 is formed so as to protrude from the bottom surface of the recess 12, and the volume of the recess 12 is large enough to accept a sample droplet adhering to the vicinity of the inlet when the sample liquid is spotted on the inlet 14. It consists of On the bottom surface of the recess 12, one or more groove portions 17 communicating with the recess 12 are formed.

  With this configuration, when the sample liquid is spotted on the inlet 14 and the sample liquid is supplied, the sample droplet 19 is attached in the vicinity of the inlet 14 as shown in FIG. . A sample droplet adhering to the vicinity of the inlet 14 due to a centrifugal force generated by attaching the analysis panel 3 to which the sample droplet 19 is attached to the analysis panel holding member 101 as it is and rotating it around the axis 11 is 10 (b), the concave portion 12 is moved in the direction of the arrow 20 and transferred to a position in front of the groove portion 17 in the concave portion 12, and finally, as shown in FIG. 10 (c). It is transferred to the inside of the groove 17 and collected there.

  That is, by providing the groove portion 17 continuously to the concave portion 12, the sample droplet 19 transferred to the concave portion 12 is further transferred to the inside of the groove portion 17 by centrifugal force, and by the capillary force inside the groove portion 17. Retained. Since it is held by capillary force, the effect of preventing the sample liquid from overflowing to the outside can be obtained even in the absence of subsequent centrifugal force.

  In this way, by using the action of the predetermined centrifugal force used for transferring the sample solution as it is, the sample droplet 19 is reliably collected in the groove portion 17, so that the sample solution is scattered outside the analysis panel 3. Can be analyzed without contamination.

  Further, since the groove portion 17 is formed at the bottom of the sample receiving portion which is the concave portion 12 on the outermost peripheral side, all the sample droplets 19 transferred to the concave portion 12 are collected at a position farther from the injection port 14. There is an effect that can be done.

  Moreover, although the cross-sectional shape of the groove part 17 in this Embodiment 3 is made into the rectangular shape, other shapes, such as a round shape, a triangle, and a polygon, may be sufficient. In any case, even if the analysis panel 3 is removed from the analysis panel holding member 101 and tilted in the direction of gravity after the analysis is completed, the groove 17 A thickness d of the opening 21 on the side of the axis 11 is formed to be 1 mm or less.

  Further, as in the case of the first embodiment, the cover 18 is provided on the analysis panel 3, the cover 18 is opened, the sample liquid is spotted on the inlet 14, and then the analysis panel is closed. By mounting 3 on the analysis panel holding member 101, the reliability of preventing the sample liquid from flowing out is further improved. That is, even if the sample liquid transferred to the concave portion 12 or the sample liquid transferred into the chamber flows out of the inlet 14 or the concave portion 12 to the side surface of the analysis panel 3 due to some influence, it is received by the cover 18 and externally received. Occurrence of a situation where it is released to In addition, after the analysis, by disposing the cover 18 without opening it, the occurrence of contamination can be prevented, which is suitable for a disposable analysis panel.

(Embodiment 4)
11 and 12 show a fourth embodiment of the present invention.
In the third embodiment, the end of the groove portion 17 is closed. However, the fourth embodiment is different from the third embodiment only in that the groove portion 17 as a passage communicates with the reagent chamber 5. Is the same.

FIG. 11 shows the vicinity of the inlet 14 of the analysis panel 3 in the fourth embodiment.
The tip of the injection port 14 communicating with the reagent chamber 5 via the holding chamber 4 is located at a position substantially equivalent to one side surface of the main body of the analysis panel 3. Only the surface on the 11 side is open, and a recess 12 that is recessed from the shaft 11 toward the outer periphery is formed. Further, the recess 12 is formed so that the cross-sectional area of the opening on the axis 11 side of the recess 12 is equal to or larger than the cross-sectional area of the opening on the outer peripheral side of the recess 12.

  Further, the injection port 14 is formed so as to protrude from the bottom surface of the recess 12, and the volume of the recess 12 is large enough to accept a sample droplet adhering to the vicinity of the injection port when the sample liquid is spotted on the injection port 14. It consists of

  In addition, the bottom surface of the recess 12 communicates with the reagent chamber 5. Specifically, one or more grooves 17 having one end communicating with the recess 12 are formed on the bottom surface of the recess 12, and the other end (end in the outer circumferential direction) of the groove 17 communicates with the reagent chamber 5. .

  In the fourth embodiment, the other end of each groove portion 17 communicates with the reagent chamber 5 after communicating with the outer peripheral side of the injection port 14, but may communicate with the reagent chamber 5 separately. Good.

  With this configuration, when the sample liquid is spotted on the inlet 14 and the sample liquid is supplied, the sample droplet 19 is attached in the vicinity of the inlet 14 as shown in FIG. . A sample droplet adhering to the vicinity of the inlet 14 due to a centrifugal force generated by attaching the analysis panel 3 to which the sample droplet 19 is attached to the analysis panel holding member 101 as it is and rotating it around the axis 11 is 12 (b), it is moved in the direction of the arrow 20 in the recess 12 and transferred to a position in front of the groove portion 17 in the recess 12, and finally as shown in FIG. 12 (c). It is transferred to the inside of the reagent chamber 5 and collected there.

  That is, by providing the groove portion 17 continuously to the concave portion 12, the sample droplet 19 transferred to the concave portion 12 is further transferred to the inside of the groove portion 17 by centrifugal force, and by the capillary force inside the groove portion 17. Since the sample liquid is held inside the reagent chamber 5 by the centrifugal force, it is possible to prevent the sample liquid from overflowing to the outside even in the absence of the subsequent centrifugal force.

  In this way, the sample droplet 19 attached in the vicinity of the inlet 14 is reliably collected in the reagent chamber 5 using the action of a predetermined centrifugal force used for transferring the sample solution as it is, so that the sample solution is scattered. This makes it possible to safely analyze the components in the sample solution without being contaminated. Furthermore, there is an effect that the sample liquid adhering to the periphery of the inlet 14 can be effectively used as a sample liquid for analysis without wasting it. This is because the same sample liquid can be replenished with respect to the sample liquid that tends to be insufficient in volume in recent years, which is a demand from the market, which is a demand for a small amount of sample liquid during sample analysis. Very beneficial.

  Further, since the groove portion 17 is formed between the concave portion 12 and the reagent chamber 5, the sample liquid transferred to the concave portion 12 can be transferred to the groove portion 17 at a position farther from the inlet 14. There is.

  Moreover, although the cross-sectional shape of the groove part 17 in this Embodiment 4 is made into the rectangular shape, other shapes, such as a round shape, a triangle, and a polygon, may be sufficient. In any case, even if the analysis panel 3 is removed from the analysis panel holding member 101 and tilted in the direction of gravity after the analysis is completed, the groove 17 A thickness d of the opening 21 on the side of the shaft center 11 is formed with a size of 1 mm or less.

  Further, as in the case of the first embodiment, the cover 18 is provided on the analysis panel 3, the cover 18 is opened, the sample liquid is spotted on the inlet 14, and then the analysis panel is closed. By mounting 3 on the analysis panel holding member 101, the reliability of preventing the sample liquid from flowing out is further improved. That is, even if the sample liquid transferred to the concave portion 12 or the sample liquid transferred into the chamber flows out of the inlet 14 or the concave portion 12 to the side surface of the analysis panel 3 due to some influence, it is received by the cover 18 and externally received. Occurrence of a situation where it is released to In addition, after the analysis, by disposing the cover 18 without opening it, the occurrence of contamination can be prevented, which is suitable for a disposable analysis panel.

(Embodiment 5)
13 to 15 show a fifth embodiment of the present invention.
In each of the embodiments described above, the analysis panel 3 is set on the analysis panel holding member 101 so that the entire analysis panel 3 is arranged on the outer peripheral side of the axis 11 of the analysis panel holding member 101. However, in the fifth embodiment, as shown in FIG. 15, a part of the analysis panel 3 protrudes to the opposite side across the axis 11 of the analysis panel holding member 101.

  As the analysis panel 3 used in this embodiment, the panel with the cover 18 is adopted among the analysis panels 3 in any of the first to fourth embodiments as shown in FIG. ing.

  14 and 15 show the state before and after the analysis panel 3 having the cover 18 is set on the analysis panel holding member 101, and the mounting position of the analysis panel 3 on the analysis panel holding member 101 is implemented. This is different from that of the first to fourth embodiments.

  Specifically, in the first to fourth embodiments, the analysis panel 3 is mounted on the outer peripheral side of the axis 11 of the analysis panel holding member 101. In the fifth embodiment, the analysis panel 3 is analyzed. The injection port 14 of the panel 3 is mounted so as to cross the axis 11 of the analysis panel holding member 101. Further, as shown in FIG. 15, if necessary, an absorbing member 22 is disposed inside the cover 18 at a position facing the inlet 14 with the cover 18 closed, with a gap 24 therebetween.

  With this configuration, when a predetermined amount of sample liquid is injected from the inlet 14 and the analysis panel 3 with the cover 18 closed is attached to the analysis panel holding member 101 and the motor 102 is driven to rotate, the holding chamber The sample solution in 4 moves in the direction of the arrow 25 from the axis 11 toward the reagent chamber 5 as in the previous embodiments. On the other hand, the sample droplet 19 adhering to the vicinity of the injection port 14 is moved in the direction of the arrow 26 by the centrifugal force and collected by the cover 18. When the absorbing member 22 is provided inside the cover 18, the absorbing member 22 is transferred to the absorbing member 22 and finally sucked by the absorbing member 22. That is, the sample droplet 19 adhering to the vicinity of the inlet 14 has a structure in which the cover 18 can be closed and prevented from being released to the outside by closing the cover 18, and the cover is opened even after analysis. Without being contaminated, there is an effect that it is suitable for a disposable analytical panel that can be disposable as it is.

  For example, the absorbent member 22 may be a nonwoven fabric made of polypropylene or paper material. The absorbent member 22 may be of any size as long as it can receive the sample liquid adhering to the vicinity of the inlet 14.

  As described above, the sample liquid 19 in the holding chamber 4 of the analysis panel 3 is used as it is to transfer the sample liquid 19 attached to the vicinity of the inlet 14 by using the centrifugal force used for transferring the sample liquid to the reagent chamber 5 and the subsequent ones. It can be reliably collected inside the cover 18.

(Embodiment 6)
16 to 19 show a sixth embodiment of the present invention.
The positional relationship between the axial center 11 and the analysis panel 3 when the analysis panel 3 having the cover 18 is attached to the analysis panel holding member 101 is the same as that in the fourth embodiment. A recess 23 is provided inside 18.

  17 and 18, with the cover 18 closed, the inlet 14 of the analysis panel 3 is set at a position away from the bottom of the cover 18 by a distance 27, and the inlet 14 is formed at the bottom of the cover 18. It is better not to touch. The amount of protrusion 28 of the recess 23 that protrudes from the bottom of the cover 18 toward the inlet 14 and surrounds the outside of the inlet 14 at a distance is such that the tip of the inlet 14 enters inside from the opening of the recess 23. Is set.

  With this configuration, when a predetermined amount of sample liquid is injected from the injection port 14 and the analysis panel 3 with the cover 18 closed is attached to the analysis panel holding member 101 and rotated by the motor 102, the holding chamber is obtained. The sample solution in 4 moves in the direction of the arrow 25 from the axis 11 toward the reagent chamber 5 as in the previous embodiments. On the other hand, the sample droplet 19 adhering to the vicinity of the inlet 14 is moved in the direction of the arrow 26 by the centrifugal force and collected in the concave portion 23 of the cover 18. That is, the sample droplet 19 adhering to the vicinity of the inlet 14 has a structure in which the cover 18 can be closed and prevented from being released to the outside by closing the cover 18, and the cover is opened even after analysis. Without being contaminated, there is an effect that it is suitable for a disposable analytical panel that can be disposable as it is.

  In addition, after injecting a predetermined amount of the sample liquid into the analysis panel 3, it is attached to the analysis panel holding member 101, and the number of rotations necessary for transferring the sample droplet is generally 1000 rpm or more. However, when hydrophilic treatment is performed with a surfactant or the like on the entire inner peripheral surface of the recess 23 and in the vicinity of the injection port 14 where the sample droplet 19 is expected to adhere, a centrifugal force due to rotation of several hundred rpm is applied. It is also possible to transfer the sample droplet to the bottom of the recess 23 and collect it.

  In addition, when the absorbing member 22 is provided in the concave portion 23 as required, as indicated by the phantom line in FIG. 18, the sample droplet 19 adhering to the vicinity of the inlet 14 is finally sucked into the absorbing member 22. Therefore, even if the opening of the concave portion 23 is tilted to the lower side where the gravity acts, there is an effect that the sample liquid can be prevented from leaking out of the absorbing member 22 or being transferred to the inlet 14 and attached again.

The structure of the recess 12 similar to that of the first embodiment may be provided in the cover 18.
In addition, a groove for holding the sample solution by capillary force may be formed in the cover 18 continuously to the recess 23. Specifically, it is configured as shown in FIGS. 19 (a), 19 (b) and 19 (c). FIG. 19A shows a cross-sectional view of the periphery of the injection port in a state where the analysis panel 3 of the same embodiment is mounted on the analysis panel holding member 101 of the analyzer. FIG. 19B shows a front view of the inside of the cover 18 from the direction of the inlet 14. FIG. 19C is a B-BB cross-sectional view in FIG. 19B, and shows a horizontal cross-sectional view in the state of FIG. As shown in FIG. 19, a large number of partition walls 29 are provided at the bottom of the recess 23. The interval between the adjacent partition walls 29 is set to an interval for sucking and holding the flying sample droplet 19 by capillary force, and is attached to the periphery of the inlet 14 by the rotation of the analysis panel holding member 101. The sample droplet 19 comes to the partition wall 29 and is held between the partition walls 29.

  According to the present invention, the measurement after the reaction between the sample solution and the analysis reagent can be performed quickly on the analysis panel. Particularly, in terms of the injection of the sample solution, the operability is very easy and the safety in terms of preventing contamination is very high. Therefore, it is useful as an analyzer for blood and the like.

1 is an external perspective view of an analysis panel according to Embodiment 1 of the present invention. The exploded perspective view of the analysis panel of the embodiment A perspective view of the analysis panel mounted on the analysis panel holding member of the analyzer Enlarged perspective view around the inlet of the analysis panel of the same embodiment Configuration diagram of the analyzer of the same embodiment External perspective view showing an example in which an openable / closable cover is attached to the analysis panel The expanded sectional view of the periphery of the inlet of the analysis panel in Embodiment 2 of this invention Explanatory drawing of the sample droplet transfer process of the embodiment Enlarged sectional view around the inlet of the analysis panel according to the third embodiment of the present invention Explanatory drawing of the sample droplet transfer process of the embodiment Enlarged sectional view around the inlet of the analysis panel according to Embodiment 4 of the present invention Explanatory drawing of the sample droplet transfer process of the embodiment External perspective view of analysis panel according to Embodiment 5 of the present invention The perspective view which shows the mounting position to the analyzer of the analysis panel of the embodiment Sectional view around the inlet with the analysis panel installed in the analyzer External perspective view with cover of analysis panel open in Embodiment 6 of the present invention The perspective view which saw through the cover in the state which closed the cover of the embodiment Sectional view around the inlet with the analysis panel installed in the analyzer (A) Cross-sectional view around the injection port, (b) Front view of the cover viewed from the injection port, and (c) Horizontal cross-sectional view of the cover in another embodiment The perspective view which shows a mode that the conventional analysis panel was mounted | worn with the analysis panel holding | maintenance part.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Upper board | substrate 2 Lower board | substrate 3 Analysis panel 4 Holding chamber 5 Reagent chamber 6 Flow path 7 Measurement chamber area | region 8 Flow path 9 Atmospheric open hole 10 Analyzer 11 Rotation center of analysis panel holding member 101 12, 23 Recessed part 14 Note Inlet 15, 16 Single-sided 17 Groove 18 Cover 19 Sample droplet 20 Sample liquid transfer direction 21 Groove opening 22 Absorbing member

Claims (25)

A sample liquid injection port is provided on one side of the panel body, and a chamber is provided in the panel main body, the chamber being connected to the injection port and transferring the sample liquid spotted on the injection port. Is an analysis panel that analyzes the components in the sample solution in the chamber by rotating the panel body in a state of being arranged on the rotation axis side,
The analysis inlet panel has a shape protruding in a direction away from the chamber from the one side surface of the panel body, and a recess is formed around the injection port in front of the one side surface of the panel body. The analysis panel according to claim 1, wherein a protruding amount of the inlet is substantially equal to one side surface of the panel main body. The analysis panel according to claim 1, wherein a cross-sectional area of the opening of the recess is equal to or greater than a cross-sectional area at the back end of the recess. The analysis panel according to claim 1, wherein the injection port protrudes with a bottom surface of the recess as a base end. 2. The analytical panel according to claim 1, wherein the volume of the recess is large enough to receive the sample liquid adhering to the vicinity of the inlet when the sample liquid is spotted on the inlet. The analysis panel according to claim 1, wherein an absorption member that absorbs the sample solution is disposed in the recess. The analysis panel according to claim 6, wherein the absorbing member is disposed at a position in contact with the sample liquid transferred to the concave portion by a centrifugal force generated by rotation around the axis. The analysis panel according to claim 1, wherein a groove portion that communicates with the concave portion and holds the sample liquid by capillary force is formed. The analysis panel according to claim 8, wherein the groove portion communicates with a bottom portion of the concave portion.   9. The analysis according to claim 8, wherein the groove has a shape in which the sample liquid transferred to the concave portion is further transferred to the inside by the centrifugal force due to the centrifugal force generated by the rotation around the axis. Panel. The analysis panel according to claim 1, wherein the recess communicates with the chamber. The passage communicating the recess and the chamber is configured such that the sample liquid adhering to the vicinity of the injection port is transferred into the chamber by a centrifugal force generated by rotation around the axis. The analysis panel according to claim 11. 2. The analysis panel according to claim 1, wherein the chamber communicated with the inlet has an analysis reagent used for analyzing blood as a sample solution. The analysis panel according to claim 1, further comprising an openable / closable cover that covers the inlet and the recess. An openable / closable cover covering the inlet and the recess;
The analysis panel according to claim 1, wherein an absorption member that absorbs the sample liquid is provided inside the cover. An openable / closable cover covering the inlet and the recess;
An absorbing member that absorbs the sample liquid is provided inside the cover,
The analysis panel according to claim 1, wherein a gap is formed between the inlet and the absorbing member. The chamber is
A holding chamber for temporarily holding the sample liquid spotted on the inlet;
A reagent chamber for holding analysis reagents necessary for analysis;
2. A measurement chamber region in which the sample liquid held in the holding chamber and the analysis reagent are transferred, mixed with each other, and measurement of the sample liquid mixed with the analysis reagent is performed. The analytical panel described. 2. The surfactant is applied to at least one of a peripheral surface of the injection port, the recess, a passage communicating the recess and the chamber, and an inner surface of the cover member. The analysis panel according to claim 12, or claim 14. A panel for analysis in which a chamber in which a sample liquid spotted on the inlet is transferred in communication with an inlet of a sample liquid provided on one side surface of the panel body is provided;
An analysis panel holding member to which the analysis panel is mounted;
The sample liquid spotted on the inlet is transferred to the chamber by the centrifugal force generated by rotating the analysis panel holding member, and the signal is detected by optically accessing the sample liquid in the chamber. An analysis device for performing analysis processing,
An openable / closable cover for covering the inlet is provided on the analysis panel,
An analysis apparatus configured to perform an analysis operation by mounting the analysis panel in a state in which the cover is closed to the analysis panel holding member so that the inlet crosses the rotational axis of the analysis panel holding member. The analyzer according to claim 19, wherein an absorption member that absorbs the sample liquid is provided inside the cover. The analyzer according to claim 19, wherein a concave portion for collecting a sample droplet adhering to the vicinity of the inlet of the analysis panel is provided inside the cover. The analyzer according to claim 21, wherein a groove for holding the sample liquid is formed in the recess by capillary force. The analyzer according to claim 19, wherein a surfactant is applied to at least one of the surface of the peripheral edge of the inlet of the analysis panel and the inner surface of the cover member. The analysis panel according to any one of claims 1 to 8,
An analysis panel holding member to which the analysis panel is mounted;
The sample liquid spotted on the inlet is transferred to the chamber by the centrifugal force generated by rotating the analysis panel holding member, and the signal is detected by optically accessing the sample liquid in the chamber. An analysis device for performing analysis processing,
The analysis panel is held by the analysis panel so that the inlet closes to the outer peripheral side of the analysis panel holding member and the injection port crosses the rotation axis of the analysis panel holding member. An analyzer configured to be mounted on a member and perform an analysis operation. A sample liquid inlet is provided on one side of the panel body, and a chamber is provided in the panel body, the chamber being connected to the inlet and transferring the sample liquid spotted on the inlet. An analysis panel for analyzing the components in the sample liquid in the chamber by rotating around the axis
The injection port has a shape protruding in a direction away from the chamber from the one side surface of the panel body,
A cover that can be opened and closed covering the inlet is provided on the panel body,
Provided inside the cover is a recess for collecting the sample droplets adhering to the vicinity of the inlet or an absorbing member for absorbing the sample droplets,
In the state in which the cover is closed, an analysis panel in which a gap is formed between the inlet and the recess, or between the inlet and the absorbing member.

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