HRP20200523T1 - Sastavljanje dnk posredovano nukleazom - Google Patents
Sastavljanje dnk posredovano nukleazom Download PDFInfo
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- HRP20200523T1 HRP20200523T1 HRP20200523TT HRP20200523T HRP20200523T1 HR P20200523 T1 HRP20200523 T1 HR P20200523T1 HR P20200523T T HRP20200523T T HR P20200523TT HR P20200523 T HRP20200523 T HR P20200523T HR P20200523 T1 HRP20200523 T1 HR P20200523T1
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- Prior art keywords
- nucleic acid
- digested
- complementary
- sequence
- oligonucleotide
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- 101710163270 Nuclease Proteins 0.000 title claims 10
- 230000001404 mediated effect Effects 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 claims 62
- 102000039446 nucleic acids Human genes 0.000 claims 62
- 150000007523 nucleic acids Chemical class 0.000 claims 62
- 230000000295 complement effect Effects 0.000 claims 30
- 108091034117 Oligonucleotide Proteins 0.000 claims 21
- 238000000034 method Methods 0.000 claims 15
- 108020004414 DNA Proteins 0.000 claims 8
- 239000003795 chemical substances by application Substances 0.000 claims 8
- 108060002716 Exonuclease Proteins 0.000 claims 6
- 102000013165 exonuclease Human genes 0.000 claims 6
- 108020005004 Guide RNA Proteins 0.000 claims 4
- 238000010459 TALEN Methods 0.000 claims 3
- 102000053602 DNA Human genes 0.000 claims 2
- 241000283984 Rodentia Species 0.000 claims 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 2
- 210000004436 artificial bacterial chromosome Anatomy 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108091008146 restriction endonucleases Proteins 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 1
- 239000012636 effector Substances 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- 108091006106 transcriptional activators Proteins 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Claims (15)
1. In vitro Postupak bešavnog sastavljanja dviju ili više dvolančanih nukleinskih kiselina, naznačen time, što uključuje:
(a) dovođenje u kontakt prve nukleinske kiseline sa najmanje jednim nukleaznim sredstvom da bi se dobila prva digestirana nukleinska kiselina kojoj je uklonjen završni dio;
(b) dovođenje u kontakt prve digestirane nukleinske kiseline sa drugom nukleinskom kiselinom, dvolančanim spojnim oligonukleotidom, i egzonukleazom, pri čemu je spojni oligonukleotid predstavljen linearnom dvolančanom DNK od oko 50 bp do oko 400 bp i uključuje:
(i) prvu komplementarnu sekvencu koja je komplementarna sa prvom digestiranom nukleinskom kiselinom;
(ii) razdjelnik; i
(iii) drugu komplementarnu sekvencu koja je komplementarna sa drugom nukleinskom kiselinom
gdje razdjelnik uključuje sekvencu identičnu sa završnim dijelom,
pri čemu između prve komplementarne sekvence i sekvence identične sa završnim dijelom nema nukleinsko-kiselinskih baza, i između druge komplementarne sekvence i sekvence identične sa završnim dijelom nema nukleinsko-kiselinskih baza, i
pri čemu egzonukleaza izlaže prvu i drugu komplementarnu sekvencu; i
(c) sastavljanje spojnog oligonukleotida sa prvom digestiranom nukleinskom kiselinom i drugom nukleinskom kiselinom, pri čemu se sastavljanjem rekonstituiše završni dio.
2. Postupak iz patentnog zahtjeva 1, naznačen time, što sastavljanje u koraku (c) uključuje:
(i) sparivanje prve komplementarne sekvence spojnog oligonukleotida sa prvom digestiranom nukleinskom kiselinom i druge komplementarne sekvence spojnog oligonukleotida sa drugom nukleinskom kiselinom; i
(ii) povezivanje spojnog oligonukleotida sa prvom digestiranom nukleinskom kiselinom i drugom nukleinskom kiselinom.
3. Postupak iz patentnog zahtjeva 1 ili 2, naznačen time, što korak (a) uključuje još i:
(i) dovođenje u kontakt druge nukleinske kiseline sa drugim nukleaznim sredstvom i egzonukleazom, pri čemu drugo nukleazno sredstvo siječe drugu nukleinsku kiselinu na drugom ciljnom mjestu da se proizvede druga digestirana nukleinska kiselina koja sadrži nukleotidnu sekvencu komplementarnu sa drugom komplementarnom sekvencom spojnog oligonukleotida, pri čemu se prva digestirana nukleinska kiselina sastavlja sa drugom digestiranom nukleinskom kiselinom; ili
(ii) dovođenje u kontakt druge nukleinske kiseline sa restrikcionim enzimom i egzonukleazom, pri čemu restrikcioni enzim siječe drugu nukleinsku kiselinu da bi se proizvela druga digestirana nukleinska kiselina koja uključuje nukleotidnu sekvencu komplementarnu sa drugom komplementarnom sekvencom u spojnom oligonukleotidu, pri čemu se prva digestirana nukleinska kiselina sastavlja sa drugom digestiranom nukleinskom kiselinom.
4. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva, naznačen time, što korak (b) uključuje još i produžavanje 3’ kraja prve digestirane nukleinske kiseline.
5. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva, naznačen time, što se spojni oligonukleotid sastavlja sa prvom digestiranom nukleinskom kiselinom i drugom nukleinskom kiselinom u istoj reakciji ili sekvencijalno.
6. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva, naznačen time, što najmanje jedno nukleazno sredstvo uključuje Cas protein i vodičku RNK (gRNK) (gRNK-Cas ompleks), zinc finger nukleazu, ili efektorsku nukleazu sličnu transkripcijskom aktivatoru (TALEN).
7. In vitro postupak bešavnog sastavljanja dviju ili više dvolančanih nukleinskih kiselina, naznačen time, što uključuje:
(a) dovođenje u kontakt prve nukleinske kiseline sa prvim nukleaznim sredstvom da bi nastala prva digestirana nukleinska kiselina sa uklonjenim završnim dijelom;
(b) dovođenje u kontakt druge nukleinske kiseline sa drugim nukleaznim sredstvom da bi nastala druga digestirana nukleinska kiselina;
(c) dovođenje u kontakt prve digestirane nukleinske kiseline i druge digestirane nukleinske kiseline sa dvolančanim spojnim oligonukleotidom i egzonukleazom, pri čemu je spojni oligonukleotid predstavljen linearnom dvolančanom DNK dugom od oko 50 bp do oko 400 bp i uključuje:
(i) prvu komplementarnu sekvencu koja je komplementarna sa prvom digestiranom nukleinskom kiselinim;
(ii) razdjelnik; i
(iii) drugu komplementarnu sekvencu koja je komplementarna sa drugom digestiranom nukleinskom kiselinom,
pri čemu razdjelnik uključuje sekvencu koja je identična sa završnim dijelom,
pri čemu između prve komplementarne sekvence i sekvence koja je identična sa završnim dijelom nema nukleinsko-kiselinskih baza, i između druge komplementarne sekvence i sekvence koja je identična sa završnim dijelom nema nukleinsko-kiselinskih baza, i
pri čemu egzonukleaza izlaže prvu i drugu komplementarnu sekvencu; i
(d) sastavljanje spojnog oligonukleotida sa prvom digestiranom nukleinskom kiselinom i drugom digestiranom nukleinskom kiselinom, pri čemu se sastavljanjem rekonstituiše završni dio.
8. Postupak iz patentnog zahtjeva 7, naznačen time, što sastavljanje u koraku (d) uključuje:
(i) sparivanje prve komplementarne sekvence spojnog oligonukleotida sa prvom digestiranom nukleinskom kiselinom i druge komplementarne sekvence spojnog oligonukleotida sa drugom digestiranom nukleinskom kiselinom; i
(ii) povezivanje spojnog oligonukleotida sa prvom digestiranom nukleinskom kiselinom i drugom digestiranom nukleinskom kiselinom.
9. Postupak iz patentnog zahtjeva 7 ili 8, naznačen time, što korak (c) uključuje još i produžavanje 3’ kraja prve digestirane nukleinske kiseline i/ili druge digestirane nukleinske kiseline.
10. Postupak iz bilo kojeg od patentnih zahtjeva 7-9, naznačen time, što se spojni oligonukleotid sastavlja sa prvom digestiranom nukleinskom kiselinom i drugom digestiranom nukleinskom kiselinom u istoj reakciji ili sekvencijalno.
11. Postupak iz bilo kojeg od patentnih zahtjeva 7-10, naznačen time, što prvo nukleazno sredstvo i/ili drugo nukleazno sredstvo uključuje Cas protein i vodičku RNK (gRNK) (gRNK-Cas kompleks), zinc finger nukleazu, ili efektorsku nukleazu sličnu transkripcijskom aktivatoru (TALEN).
12. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva, naznačen time, što:
(I) prva komplementarna sekvenca spojnog oligonukleotida ima između 15 i 120 komplementarnih baza i druga komplementarna sekvenca spojnog oligonukleotida ima između 15 i 120 komplementarnih baza; i/ili
(II) završni dio ima najmanje 20 bp; i/ili
(III) razdjelnik ima od oko 20 bp do oko 120 bp.
13. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva, naznačen time, što:
(I) prva nukleinska kiselina, druga nukleinska kiselina, ili obje nukleinske kiseline uključuju bakterijski umjetni kromosom; i/ili
(II) prva nukleinska kiselina, druga nukleinska kiselina, ili obje nukleinske kiseline uključuju humanu DNK, glodavačku DNK, sintetsku DNK, ili njihovu kombinaciju; i/ili
(III) prva nukleinska kiselina, druga nukleinska kiselina, ili obje nukleinske kiseline imaju najmanje10 kb; i/ili
(IV) prva nukleinska kiselina, druga nukleinska kiselina, ili obje nukleinske kiseline imaju najmanje 10 kb, uključuju bakterijski umjetni kromosom, i uključuju humanu DNK, glodavačku DNK, sintetsku DNK, ili njihovu kombinaciju.
14. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva, naznačen time, što se sastavljaju najmanje tri nukleinske kiseline.
15. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva, naznačen time, što:
(I) spojni oligonukleotid ima od oko 100 bp do oko 300 bp; i/ili
(II) prva komplementarna sekvenca spojnog oligonukleotida ima između 20 i 80 komplementarnih baza i druga komplementarna sekvenca spojnog oligonukleotida ima između 20 i 80 komplementarnih baza.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462015809P | 2014-06-23 | 2014-06-23 | |
| US201462016400P | 2014-06-24 | 2014-06-24 | |
| US201462036983P | 2014-08-13 | 2014-08-13 | |
| EP18162656.5A EP3354732B1 (en) | 2014-06-23 | 2015-06-23 | Nuclease-mediated dna assembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20200523T1 true HRP20200523T1 (hr) | 2020-08-07 |
Family
ID=53525285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20200523TT HRP20200523T1 (hr) | 2014-06-23 | 2020-03-30 | Sastavljanje dnk posredovano nukleazom |
Country Status (25)
| Country | Link |
|---|---|
| US (6) | US9738897B2 (hr) |
| EP (3) | EP3155099B1 (hr) |
| JP (4) | JP6336140B2 (hr) |
| KR (2) | KR101822902B1 (hr) |
| CN (2) | CN112029787B (hr) |
| AU (3) | AU2015280120B2 (hr) |
| BR (1) | BR112016030145A8 (hr) |
| CA (1) | CA2953499C (hr) |
| CY (2) | CY1120520T1 (hr) |
| DK (2) | DK3354732T3 (hr) |
| ES (2) | ES2781323T3 (hr) |
| HK (1) | HK1256688A1 (hr) |
| HR (1) | HRP20200523T1 (hr) |
| HU (1) | HUE049405T2 (hr) |
| IL (1) | IL249612B (hr) |
| LT (1) | LT3354732T (hr) |
| MX (2) | MX2016016905A (hr) |
| NZ (1) | NZ765591A (hr) |
| PL (2) | PL3155099T3 (hr) |
| PT (2) | PT3155099T (hr) |
| RS (1) | RS60366B1 (hr) |
| RU (1) | RU2707911C2 (hr) |
| SG (2) | SG10201803444YA (hr) |
| SI (1) | SI3354732T1 (hr) |
| WO (1) | WO2015200334A1 (hr) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| LT3354732T (lt) | 2014-06-23 | 2020-04-10 | Regeneron Pharmaceuticals, Inc. | Nukleazės tarpininkaujamas dnr surinkimas |
| EP3167071B1 (en) * | 2014-07-09 | 2020-10-07 | Gen9, Inc. | Compositions and methods for site-directed dna nicking and cleaving |
| EP4079847A1 (en) | 2014-07-30 | 2022-10-26 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| JP7068821B2 (ja) | 2014-12-03 | 2022-05-17 | アジレント・テクノロジーズ・インク | 化学修飾を有するガイドｒｎａ |
| WO2016109255A1 (en) * | 2014-12-30 | 2016-07-07 | University Of South Florida | Methods and compositions for cloning into large vectors |
| JP6873911B2 (ja) | 2015-04-06 | 2021-05-19 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | 初代細胞において標的核酸の遺伝子調節を誘導するためにインビトロで行う方法 |
| AU2016270587B2 (en) | 2015-05-29 | 2020-12-24 | Regeneron Pharmaceuticals, Inc. | Non-human animals having a disruption in a C9ORF72 locus |
| EP3365356B1 (en) | 2015-10-23 | 2023-06-28 | President and Fellows of Harvard College | Nucleobase editors and uses thereof |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| WO2017100376A2 (en) | 2015-12-07 | 2017-06-15 | Zymergen, Inc. | Promoters from corynebacterium glutamicum |
| IL260526B2 (en) | 2016-01-13 | 2023-10-01 | Regeneron Pharma | Rodents with an engineered heavy chain diversity region |
| HUE061619T2 (hu) | 2016-06-03 | 2023-07-28 | Regeneron Pharma | Exogén terminális dezoxinukleotid-transzferázt expresszáló rágcsálók |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| WO2018005655A2 (en) | 2016-06-30 | 2018-01-04 | Zymergen Inc. | Methods for generating a bacterial hemoglobin library and uses thereof |
| US10544411B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
| WO2018013990A1 (en) * | 2016-07-15 | 2018-01-18 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| JP2019523009A (ja) | 2016-07-29 | 2019-08-22 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | Ｃ末端切断型フィブリリン−１の発現をもたらす変異を有するマウス |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018048827A1 (en) * | 2016-09-07 | 2018-03-15 | Massachusetts Institute Of Technology | Rna-guided endonuclease-based dna assembly |
| CN110023504B (zh) | 2016-09-15 | 2023-05-09 | 阿谢尔德克斯有限责任公司 | 用于分析无细胞dna的核酸样品制备方法 |
| JP6997772B2 (ja) | 2016-09-15 | 2022-01-18 | アーチャーディーエックス， エルエルシー | 核酸サンプル調製の方法 |
| US10781453B2 (en) | 2016-09-30 | 2020-09-22 | Regeneron Pharmaceuticals, Inc. | Non-human animals having a hexanucleotide repeat expansion in a C9ORF72 locus |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| EP3535405B1 (en) | 2016-11-02 | 2022-12-21 | ArcherDX, LLC | Methods of nucleic acid sample preparation for immune repertoire sequencing |
| PT3407709T (pt) | 2016-11-04 | 2020-08-20 | Regeneron Pharma | Animais não humanos possuindo um lócus da cadeia leve lambda de immunoglobulina modificada |
| CN110291198A (zh) * | 2016-12-08 | 2019-09-27 | 因特利亚治疗公司 | 经修饰的指导rna |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
| CA3049989A1 (en) * | 2017-02-10 | 2018-08-16 | Zymergen Inc. | A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| JP7191388B2 (ja) | 2017-03-23 | 2022-12-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸によってプログラム可能なｄｎａ結合蛋白質を含む核酸塩基編集因子 |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CA3062334A1 (en) * | 2017-06-01 | 2018-12-06 | Myriad Women's Health, Inc. | Preparation of concatenated polynucleotides |
| CA3066744A1 (en) * | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| US10081829B1 (en) * | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
| CN111094573A (zh) * | 2017-07-12 | 2020-05-01 | 梅约医学教育与研究基金会 | 有效靶向敲入或基因置换的材料和方法 |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| KR20240145526A (ko) | 2017-07-31 | 2024-10-07 | 리제너론 파마슈티칼스 인코포레이티드 | Cas-형질전환 마우스 배아 줄기 세포 및 마우스 및 이것의 용도 |
| WO2019028023A2 (en) | 2017-07-31 | 2019-02-07 | Regeneron Pharmaceuticals, Inc. | METHODS AND COMPOSITIONS FOR EVALUATING CRISPR / CAS MEDIATED DISRUPTION OR EXCISION AND CRISPR / CAS INDUCED RECOMBINATION USING IN VIVO EXOGENIC DONOR NUCLEIC ACID |
| BR112020003439A2 (pt) * | 2017-08-21 | 2020-08-25 | Tokushima University | tecnologia de alteração específica de sequência alvo usando o reconhecimento alvo nucleotídico |
| EP3676376A2 (en) | 2017-08-30 | 2020-07-08 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| NZ761329A (en) | 2017-09-29 | 2023-04-28 | Regeneron Pharma | Non-human animals comprising a humanized ttr locus and methods of use |
| AU2018352592A1 (en) | 2017-10-16 | 2020-06-04 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| SG11202002456WA (en) | 2017-11-30 | 2020-04-29 | Regeneron Pharma | Non-human animals comprising a humanized trkb locus |
| AU2018381316A1 (en) | 2017-12-05 | 2020-04-23 | Regeneron Pharmaceuticals, Inc. | Non-human animals having an engineered immunoglobulin lambda light chain and uses thereof |
| CN116349651A (zh) | 2018-03-19 | 2023-06-30 | 瑞泽恩制药公司 | 使用crispr/cas系统对动物进行转录调制 |
| CA3093850A1 (en) | 2018-03-26 | 2019-10-03 | Regeneron Pharmaceuticals, Inc. | Humanized rodents for testing therapeutic agents |
| WO2019204531A1 (en) * | 2018-04-18 | 2019-10-24 | Ligandal, Inc. | Methods and compositions for genome editing |
| WO2019217785A1 (en) * | 2018-05-10 | 2019-11-14 | St. Jude Children's Research Hospital, Inc. | High-throughput method for characterizing the genome-wide activity of editing nucleases in vitro |
| WO2019213910A1 (en) * | 2018-05-10 | 2019-11-14 | Syngenta Participations Ag | Methods and compositions for targeted editing of polynucleotides |
| SG11202012084WA (en) | 2018-06-14 | 2021-01-28 | Regeneron Pharma | Non-human animals capable of dh-dh rearrangement in the immunoglobulin heavy chain coding sequences |
| WO2020086144A2 (en) * | 2018-08-15 | 2020-04-30 | Zymergen Inc. | APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING |
| KR20210105914A (ko) | 2018-12-20 | 2021-08-27 | 리제너론 파마슈티칼스 인코포레이티드 | 뉴클레아제-매개 반복부 팽창 |
| WO2020191243A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US11737435B2 (en) | 2019-04-04 | 2023-08-29 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized coagulation factor 12 locus |
| RU2771374C1 (ru) | 2019-04-04 | 2022-05-04 | Редженерон Фармасьютикалс, Инк. | Способы для бесшовного внесения целевых модификаций в направленные векторы |
| GB201905651D0 (en) * | 2019-04-24 | 2019-06-05 | Lightbio Ltd | Nucleic acid constructs and methods for their manufacture |
| WO2020224987A1 (en) * | 2019-05-06 | 2020-11-12 | Dsm Ip Assets B.V. | Multipartite crispr donor |
| WO2020247452A1 (en) | 2019-06-04 | 2020-12-10 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized ttr locus with a beta-slip mutation and methods of use |
| AU2020289554A1 (en) | 2019-06-05 | 2021-11-18 | Regeneron Pharmaceuticals, Inc. | Non-human animals having a limited lambda light chain repertoire expressed from the kappa locus and uses thereof |
| TW202112229A (zh) | 2019-06-07 | 2021-04-01 | 美商雷傑納榮製藥公司 | 包含人化白蛋白基因座之非人的動物 |
| JP2022539338A (ja) | 2019-06-25 | 2022-09-08 | イナリ アグリカルチャー テクノロジー， インコーポレイテッド | 改良された相同性依存性修復ゲノム編集 |
| CN112175939A (zh) * | 2019-07-03 | 2021-01-05 | 华大青兰生物科技(无锡)有限公司 | 一种基于同源序列的核酸拼接方法及其应用 |
| GB201913898D0 (en) * | 2019-09-26 | 2019-11-13 | Lightbio Ltd | Nucleic acid construct |
| WO2021108363A1 (en) | 2019-11-25 | 2021-06-03 | Regeneron Pharmaceuticals, Inc. | Crispr/cas-mediated upregulation of humanized ttr allele |
| US20230091690A1 (en) * | 2019-12-30 | 2023-03-23 | The Broad Institute, Inc. | Guided excision-transposition systems |
| CA3169272A1 (en) | 2020-01-28 | 2021-08-05 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized pnpla3 locus and methods of use |
| WO2021158883A1 (en) | 2020-02-07 | 2021-08-12 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized klkb1 locus and methods of use |
| CN111334523A (zh) * | 2020-03-13 | 2020-06-26 | 天津大学 | 一种大尺度dna的体内多轮迭代组装方法 |
| WO2021195079A1 (en) | 2020-03-23 | 2021-09-30 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use |
| CA3177481A1 (en) | 2020-05-08 | 2021-11-11 | David R. Liu | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| US20240317849A1 (en) | 2020-12-23 | 2024-09-26 | Regeneron Pharmaceuticals, Inc. | Nucleic acids encoding anchor modified antibodies and uses thereof |
| WO2022187125A1 (en) * | 2021-03-01 | 2022-09-09 | The Regents Of The University Of California | Methods for generating a crispr array |
| AU2022343300A1 (en) | 2021-09-10 | 2024-04-18 | Agilent Technologies, Inc. | Guide rnas with chemical modification for prime editing |
| CN113782103B (zh) * | 2021-10-26 | 2024-07-09 | 大连大学 | 一种基于组合式酶切机制的dna矩阵处理方法 |
| EP4423271A2 (en) | 2021-10-28 | 2024-09-04 | Regeneron Pharmaceuticals, Inc. | Crispr/cas-related methods and compositions for knocking out c5 |
| WO2023108047A1 (en) | 2021-12-08 | 2023-06-15 | Regeneron Pharmaceuticals, Inc. | Mutant myocilin disease model and uses thereof |
| US20230257790A1 (en) * | 2022-01-31 | 2023-08-17 | Integrated Dna Technologies, Inc. | Recombination-based dna assembly methods and compositions |
| WO2023150798A1 (en) | 2022-02-07 | 2023-08-10 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for defining optimal treatment timeframes in lysosomal disease |
| CN118613498A (zh) | 2022-02-11 | 2024-09-06 | 瑞泽恩制药公司 | 用于筛选4R Tau靶向剂的组合物和方法 |
| WO2023235725A2 (en) | 2022-05-31 | 2023-12-07 | Regeneron Pharmaceuticals, Inc. | Crispr-based therapeutics for c9orf72 repeat expansion disease |
| WO2024073679A1 (en) | 2022-09-29 | 2024-04-04 | Regeneron Pharmaceuticals, Inc. | Correction of hepatosteatosis in humanized liver animals through restoration of il6/il6r/gp130 signaling in human hepatocytes |
| CN117987436B (zh) * | 2024-04-03 | 2024-06-25 | 南京鸿明生物科技有限公司 | 一种双链靶dna序列的制备方法 |
Family Cites Families (62)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US6599692B1 (en) | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
| US20030104526A1 (en) | 1999-03-24 | 2003-06-05 | Qiang Liu | Position dependent recognition of GNN nucleotide triplets by zinc fingers |
| US7105348B2 (en) | 2000-10-31 | 2006-09-12 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| US6586251B2 (en) | 2000-10-31 | 2003-07-01 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| US20050144655A1 (en) | 2000-10-31 | 2005-06-30 | Economides Aris N. | Methods of modifying eukaryotic cells |
| US6596541B2 (en) | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| EP1341914A2 (en) | 2000-12-07 | 2003-09-10 | Sangamo Biosciences Inc. | Regulation of angiogenesis with zinc finger proteins |
| WO2002057308A2 (en) | 2001-01-22 | 2002-07-25 | Sangamo Biosciences, Inc. | Zinc finger polypeptides and their use |
| AU2002241946B2 (en) | 2001-01-22 | 2007-04-26 | Sangamo Therapeutics, Inc. | Modified zinc finger binding proteins |
| DE60310202T2 (de) | 2002-01-23 | 2007-11-22 | The University Of Utah Research Foundation, Salt Lake City | Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen |
| US8206965B2 (en) | 2002-03-15 | 2012-06-26 | Cellectis S.A. | Hybrid and single chain meganucleases and use thereof |
| ATE531796T1 (de) | 2002-03-21 | 2011-11-15 | Sangamo Biosciences Inc | Verfahren und zusammensetzungen zur verwendung von zinkfinger-endonukleasen zur verbesserung der homologen rekombination |
| US9447434B2 (en) | 2002-09-05 | 2016-09-20 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
| AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
| EP1591521A1 (en) | 2004-04-30 | 2005-11-02 | Cellectis | I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof |
| CA2579677A1 (en) | 2004-09-16 | 2006-03-30 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
| JP2008535484A (ja) | 2005-03-15 | 2008-09-04 | セレクティス | 特異性が改変されたＩ−ＣｒｅＩメガヌクレアーゼ変異型、その作製方法及びその使用 |
| WO2006097784A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
| PT2412812E (pt) | 2006-12-14 | 2015-04-30 | Dow Agrosciences Llc | Proteínas com dedos de zinco não canónicas optimizadas |
| EP2255013B1 (en) | 2008-02-15 | 2016-06-08 | Synthetic Genomics, Inc. | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules |
| US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
| EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
| WO2010107493A2 (en) | 2009-03-20 | 2010-09-23 | Sangamo Biosciences, Inc. | Modification of cxcr4 using engineered zinc finger proteins |
| US8772008B2 (en) | 2009-05-18 | 2014-07-08 | Sangamo Biosciences, Inc. | Methods and compositions for increasing nuclease activity |
| US20120178647A1 (en) | 2009-08-03 | 2012-07-12 | The General Hospital Corporation | Engineering of zinc finger arrays by context-dependent assembly |
| US8354389B2 (en) | 2009-08-14 | 2013-01-15 | Regeneron Pharmaceuticals, Inc. | miRNA-regulated differentiation-dependent self-deleting cassette |
| PL2494047T3 (pl) | 2009-10-29 | 2017-05-31 | Regeneron Pharmaceuticals, Inc. | Allele wielofunkcyjne |
| DK2510096T4 (en) | 2009-12-10 | 2018-05-14 | Univ Iowa State Res Found Inc | NUMBER EFFECTOR-MEDIATED DNA MODIFICATION |
| WO2011101696A1 (en) | 2010-02-18 | 2011-08-25 | Cellectis | Improved meganuclease recombination system |
| GB201009732D0 (en) | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| WO2012051327A2 (en) | 2010-10-12 | 2012-04-19 | Cornell University | Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements |
| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| TR201806812T4 (tr) | 2012-05-25 | 2018-06-21 | Charpentier Emmanuelle | Rna-yönlendirmeli hedef dna modifikasyonu için ve rna-yönlendirmeli transkripsiyon modifikasyonu için yöntemler ve bileşimler. |
| WO2014065596A1 (en) | 2012-10-23 | 2014-05-01 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| LT3138912T (lt) * | 2012-12-06 | 2019-02-25 | Sigma-Aldrich Co. Llc | Genomo modifikavimas ir reguliavimas crispr pagrindu |
| RU2721275C2 (ru) | 2012-12-12 | 2020-05-18 | Те Брод Инститьют, Инк. | Доставка, конструирование и оптимизация систем, способов и композиций для манипуляции с последовательностями и применения в терапии |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| IL239326B1 (en) | 2012-12-17 | 2024-10-01 | Harvard College | RNA-guided human genome engineering |
| JP2016507244A (ja) | 2013-02-27 | 2016-03-10 | ヘルムホルツ・ツェントルム・ミュンヒェン・ドイチェス・フォルシュンクスツェントルム・フューア・ゲズントハイト・ウント・ウムベルト（ゲーエムベーハー）Ｈｅｌｍｈｏｌｔｚ Ｚｅｎｔｒｕｍ ＭｕｅｎｃｈｅｎＤｅｕｔｓｃｈｅｓ Ｆｏｒｓｃｈｕｎｇｓｚｅｎｔｒｕｍ ｆｕｅｒ Ｇｅｓｕｎｄｈｅｉｔ ｕｎｄ Ｕｍｗｅｌｔ （ＧｍｂＨ） | Ｃａｓ９ヌクレアーゼによる卵母細胞における遺伝子編集 |
| WO2014143381A1 (en) | 2013-03-09 | 2014-09-18 | Agilent Technologies, Inc. | Methods of in vivo engineering of large sequences using multiple crispr/cas selections of recombineering events |
| WO2014165825A2 (en) | 2013-04-04 | 2014-10-09 | President And Fellows Of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
| US10421957B2 (en) | 2013-07-29 | 2019-09-24 | Agilent Technologies, Inc. | DNA assembly using an RNA-programmable nickase |
| ES2681622T3 (es) | 2013-09-18 | 2018-09-14 | Kymab Limited | Métodos, células y organismos |
| KR102274445B1 (ko) | 2013-12-19 | 2021-07-08 | 아미리스 인코퍼레이티드 | 게놈 삽입을 위한 방법 |
| WO2015116686A1 (en) | 2014-01-29 | 2015-08-06 | Agilent Technologies, Inc. | Cas9-based isothermal method of detection of specific dna sequence |
| JP2016006930A (ja) | 2014-06-20 | 2016-01-14 | ソニー株式会社 | 撮像装置および撮像方法 |
| LT3354732T (lt) | 2014-06-23 | 2020-04-10 | Regeneron Pharmaceuticals, Inc. | Nukleazės tarpininkaujamas dnr surinkimas |
| EP3167071B1 (en) | 2014-07-09 | 2020-10-07 | Gen9, Inc. | Compositions and methods for site-directed dna nicking and cleaving |
| US20160053272A1 (en) | 2014-07-18 | 2016-02-25 | Whitehead Institute For Biomedical Research | Methods Of Modifying A Sequence Using CRISPR |
| US11071289B2 (en) | 2014-08-14 | 2021-07-27 | Biocytogen Boston Corp | DNA knock-in system |
| CA2958082A1 (en) | 2014-08-25 | 2016-03-03 | Curza Global, Llc | Method of producing n-alkyl polyamines |
| US9447445B2 (en) | 2014-08-27 | 2016-09-20 | New England Biolabs, Inc. | Synthon formation |
| CN104357438B (zh) * | 2014-09-23 | 2020-04-24 | 中国科学院天津工业生物技术研究所 | 一种dna组装和克隆的方法 |
| CN107278227B (zh) | 2014-12-16 | 2021-05-28 | C3J治疗公司 | 用于体外病毒基因组工程的组合物和方法 |
| WO2016109255A1 (en) | 2014-12-30 | 2016-07-07 | University Of South Florida | Methods and compositions for cloning into large vectors |
| WO2016130697A1 (en) | 2015-02-11 | 2016-08-18 | Memorial Sloan Kettering Cancer Center | Methods and kits for generating vectors that co-express multiple target molecules |
| WO2018013990A1 (en) | 2016-07-15 | 2018-01-18 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| WO2018048827A1 (en) | 2016-09-07 | 2018-03-15 | Massachusetts Institute Of Technology | Rna-guided endonuclease-based dna assembly |
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