HK1142362A1 - Polyanion for improved nucleic acid amplification - Google Patents

Polyanion for improved nucleic acid amplification

Info

Publication number
HK1142362A1
HK1142362A1 HK10108684.2A HK10108684A HK1142362A1 HK 1142362 A1 HK1142362 A1 HK 1142362A1 HK 10108684 A HK10108684 A HK 10108684A HK 1142362 A1 HK1142362 A1 HK 1142362A1
Authority
HK
Hong Kong
Prior art keywords
polyanion
nucleic acid
acid amplification
improved nucleic
group
Prior art date
Application number
HK10108684.2A
Other languages
English (en)
Chinese (zh)
Inventor
Waltraud Ankenbauer
Dieter Heindl
Renate Kolb
Frank Laue
Eva Walter
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1142362A1 publication Critical patent/HK1142362A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
HK10108684.2A 2008-09-09 2010-09-13 Polyanion for improved nucleic acid amplification HK1142362A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08015812 2008-09-09

Publications (1)

Publication Number Publication Date
HK1142362A1 true HK1142362A1 (en) 2010-12-03

Family

ID=40481991

Family Applications (1)

Application Number Title Priority Date Filing Date
HK10108684.2A HK1142362A1 (en) 2008-09-09 2010-09-13 Polyanion for improved nucleic acid amplification

Country Status (8)

Country Link
US (1) US7910720B2 (xx)
EP (1) EP2163556B1 (xx)
JP (1) JP5436105B2 (xx)
CN (1) CN101671672B (xx)
AT (1) ATE517116T1 (xx)
CA (1) CA2678229C (xx)
ES (1) ES2367469T3 (xx)
HK (1) HK1142362A1 (xx)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009055039A1 (en) * 2007-10-25 2009-04-30 Canon U.S. Life Sciences, Inc High-resolution melting analysis
JP6174999B2 (ja) 2010-10-04 2017-08-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｐｃｒ反応緩衝液中での細胞溶解のための方法
CA2810316A1 (en) 2010-10-04 2012-04-12 F. Hoffmann-La Roche Ag Method for cell lysis and pcr within the same reaction vessel
CA2810291C (en) 2010-10-04 2016-09-27 F. Hoffmann-La Roche Ag Method for cell lysis and amplification of rna in a rt-pcr reaction buffer

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5449603A (en) 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
AU653712B2 (en) 1990-02-16 1994-10-13 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE4110808A1 (de) 1991-04-04 1992-10-08 Basf Ag Indolizintrimethinfarbstoffe
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
JP3087152B2 (ja) * 1993-09-08 2000-09-11 富士通株式会社 樹脂フィルム多層回路基板の製造方法
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US6033851A (en) 1994-12-09 2000-03-07 Wakunaga Seiyaku Kabushiki Kaisha Method for suppressing nonspecific hybridization in primer extension method
CA2168712A1 (en) 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
CA2176193A1 (en) 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
WO1996041010A1 (en) 1995-06-07 1996-12-19 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
EP0910668A4 (en) 1996-06-03 2003-02-19 Univ Alberta METHOD FOR DETECTING REASSEMBLED DNA
ES2304573T3 (es) 1996-06-04 2008-10-16 University Of Utah Research Foundation Recipiente para llevar a cabo y controlar procesos biologicos.
CA2658290C (en) 1996-06-04 2012-04-10 University Of Utah Research Foundation Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
DE19913923A1 (de) 1999-03-26 2000-09-28 Amphenol Tuchel Elect Kontaktiereinrichtung für eine SIM-Karte
AU5008900A (en) 1999-05-12 2000-11-21 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6635427B2 (en) 2000-08-11 2003-10-21 University Of Utah Research Foundation Single-labeled oligonucleotide probes for homogeneous nucleic acid sequence analysis
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US6403341B1 (en) 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US20060263811A1 (en) 2005-05-03 2006-11-23 Geunsook Jeon Materials and kits for use in hot-start PCR, and methods of amplifying nucleic acids in a polymerase chain reaction
WO2007096182A1 (en) 2006-02-27 2007-08-30 Roche Diagnostics Gmbh Pcr hot start by magnesium sequestration

Also Published As

Publication number Publication date
US7910720B2 (en) 2011-03-22
US20100255547A1 (en) 2010-10-07
CA2678229A1 (en) 2010-03-09
EP2163556A1 (en) 2010-03-17
JP2010063456A (ja) 2010-03-25
CA2678229C (en) 2017-03-07
JP5436105B2 (ja) 2014-03-05
CN101671672B (zh) 2015-05-13
CN101671672A (zh) 2010-03-17
EP2163556B1 (en) 2011-07-20
ES2367469T3 (es) 2011-11-03
ATE517116T1 (de) 2011-08-15

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