GB877898A - A new or improved method of liberating enzymes - Google Patents

A new or improved method of liberating enzymes

Info

Publication number
GB877898A
GB877898A GB3739456A GB3739456A GB877898A GB 877898 A GB877898 A GB 877898A GB 3739456 A GB3739456 A GB 3739456A GB 3739456 A GB3739456 A GB 3739456A GB 877898 A GB877898 A GB 877898A
Authority
GB
United Kingdom
Prior art keywords
yeast
fungi
enzymes
suspension
diastase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3739456A
Inventor
Eric George Watson
George Anthony Dummett
George Henry Botham
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SPX Flow Technology Crawley Ltd
Original Assignee
APV Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by APV Corp Ltd filed Critical APV Corp Ltd
Priority to GB3739456A priority Critical patent/GB877898A/en
Publication of GB877898A publication Critical patent/GB877898A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Enzymes are liberated from the cells of yeast and other fungi by impacting a liquid suspension of the yeast or other fungi against a surface, preferably a metal surface, by projecting the suspension under pressure towards the surface through a small orifice and regulating the extent of the liberation of the enzymes by varying the pressure at which the suspension is projected. The method is used in the preparation of invertase from yeast and diastase from fungi.ALSO:Enzymes are liberated from the cells of yeast and other fungi by imparting a liquid suspension of the yeast or other fungi against a surface, preferably a metal surface, by projecting the suspension under pressure towards the surface through a small orifice and regulating the extent of the liberation of the enzymes by varying the pressure at which the suspension is projected. The method may be used to produce homogenized yeast suspensions for use in the production of (a) invert sugar from cane sugar; (b) "high test," molasses from cane sugar syrups; (c) baker's yeast; (d) yeast extract, and (e) beer from immature beer by secondary fermentation of reduced duration. The method may also be used in the production of diastase from fungi.
GB3739456A 1956-12-06 1956-12-06 A new or improved method of liberating enzymes Expired GB877898A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB3739456A GB877898A (en) 1956-12-06 1956-12-06 A new or improved method of liberating enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3739456A GB877898A (en) 1956-12-06 1956-12-06 A new or improved method of liberating enzymes

Publications (1)

Publication Number Publication Date
GB877898A true GB877898A (en) 1961-09-20

Family

ID=10396140

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3739456A Expired GB877898A (en) 1956-12-06 1956-12-06 A new or improved method of liberating enzymes

Country Status (1)

Country Link
GB (1) GB877898A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2001977A1 (en) * 1968-02-15 1969-10-03 Astra Ab Process for the prepn. of cell-free penicillin-acylase by fermenting a strain of E. coli which produces this enzyme. The intracellular enzyme is separated form
JPS5025539B1 (en) * 1971-07-14 1975-08-25
JPS5132766A (en) * 1974-09-07 1976-03-19 Idemitsu Kosan Co
WO1997012959A1 (en) * 1995-10-06 1997-04-10 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
US5721120A (en) * 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
WO2005113757A1 (en) * 2004-05-18 2005-12-01 Glaxo Group Limited Apparatus for the disruption of animal cells
US7056723B2 (en) 2001-12-20 2006-06-06 Bavarian Nordic A/S Method for the recovery and purification of poxviruses from infected cells

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2001977A1 (en) * 1968-02-15 1969-10-03 Astra Ab Process for the prepn. of cell-free penicillin-acylase by fermenting a strain of E. coli which produces this enzyme. The intracellular enzyme is separated form
JPS5025539B1 (en) * 1971-07-14 1975-08-25
JPS5132766A (en) * 1974-09-07 1976-03-19 Idemitsu Kosan Co
JPS5428467B2 (en) * 1974-09-07 1979-09-17
WO1997012959A1 (en) * 1995-10-06 1997-04-10 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
US5721120A (en) * 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
US7056723B2 (en) 2001-12-20 2006-06-06 Bavarian Nordic A/S Method for the recovery and purification of poxviruses from infected cells
WO2005113757A1 (en) * 2004-05-18 2005-12-01 Glaxo Group Limited Apparatus for the disruption of animal cells

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