GB8510219D0 - Isolation of fermentation products - Google Patents

Isolation of fermentation products

Info

Publication number
GB8510219D0
GB8510219D0 GB858510219A GB8510219A GB8510219D0 GB 8510219 D0 GB8510219 D0 GB 8510219D0 GB 858510219 A GB858510219 A GB 858510219A GB 8510219 A GB8510219 A GB 8510219A GB 8510219 D0 GB8510219 D0 GB 8510219D0
Authority
GB
United Kingdom
Prior art keywords
peptide
yeast
heterologous protein
protein
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
GB858510219A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bass PLC
Original Assignee
Bass PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bass PLC filed Critical Bass PLC
Priority to GB858510219A priority Critical patent/GB8510219D0/en
Publication of GB8510219D0 publication Critical patent/GB8510219D0/en
Priority to AT86303039T priority patent/ATE47426T1/en
Priority to ZA863005A priority patent/ZA863005B/en
Priority to DK184486A priority patent/DK184486A/en
Priority to FI861696A priority patent/FI86079C/en
Priority to EP86303039A priority patent/EP0201239B1/en
Priority to ES554234A priority patent/ES8706828A1/en
Priority to AU56448/86A priority patent/AU585006B2/en
Priority to GB08609776A priority patent/GB2175590B/en
Priority to DE8686303039T priority patent/DE3666459D1/en
Priority to JP61093172A priority patent/JPS61282097A/en
Priority to SG872/91A priority patent/SG87291G/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C12C11/003—Fermentation of beerwort
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C12C5/006—Beta-glucanase or functionally equivalent enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Alcoholic Beverages (AREA)

Abstract

The invention provides a process for the production of ethanol and a protein or peptide which is heterologous to yeast which comprises fermenting an aqueous carbohydrate-containing medium with an industrial yeast strain which has been genetically modified to be capable of expressing a heterologous protein or peptide, under conditions such that the yeast multiplies but no expression of the said heterologous protein or peptide takes place, recovering the ethanol so-formed, inducing expression of the said protein or peptide by the yeast, and obtaining the said heterologous protein or peptide therefrom. The process may be applied to the industrial production of alcoholic beverages such as beer or distilled alcohol. The yeast inevitably obtained as a by-product in the process has improved value because it provides a source of the heterologous protein or peptide. Heterologous protein and peptides which may be produced by the new process include enzymes such as beta-lactamase, beta-glucanase and beta-galactosidase and proteins of therapeutic value such as human serum albumin.
GB858510219A 1985-04-22 1985-04-22 Isolation of fermentation products Pending GB8510219D0 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
GB858510219A GB8510219D0 (en) 1985-04-22 1985-04-22 Isolation of fermentation products
JP61093172A JPS61282097A (en) 1985-04-22 1986-04-22 Separation of fermented product
FI861696A FI86079C (en) 1985-04-22 1986-04-22 Process for producing ethanol and a protein or peptide which is heterologous to yeast
ZA863005A ZA863005B (en) 1985-04-22 1986-04-22 Isolation of fermentation products
DK184486A DK184486A (en) 1985-04-22 1986-04-22 PROCEDURE FOR THE ISOLATION OF FERMENTATION PRODUCTS
AT86303039T ATE47426T1 (en) 1985-04-22 1986-04-22 PROCESSES FOR ISOLATION OF FERMENTATION PRODUCTS.
EP86303039A EP0201239B1 (en) 1985-04-22 1986-04-22 Process for the isolation of fermentation products
ES554234A ES8706828A1 (en) 1985-04-22 1986-04-22 Process for the isolation of fermentation products.
AU56448/86A AU585006B2 (en) 1985-04-22 1986-04-22 Process for the isolation of fermentation products
GB08609776A GB2175590B (en) 1985-04-22 1986-04-22 Process for the isolation of fermentation products
DE8686303039T DE3666459D1 (en) 1985-04-22 1986-04-22 Process for the isolation of fermentation products
SG872/91A SG87291G (en) 1985-04-22 1991-10-18 Process for the isolation of fermentation products

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB858510219A GB8510219D0 (en) 1985-04-22 1985-04-22 Isolation of fermentation products

Publications (1)

Publication Number Publication Date
GB8510219D0 true GB8510219D0 (en) 1985-05-30

Family

ID=10578009

Family Applications (2)

Application Number Title Priority Date Filing Date
GB858510219A Pending GB8510219D0 (en) 1985-04-22 1985-04-22 Isolation of fermentation products
GB08609776A Expired GB2175590B (en) 1985-04-22 1986-04-22 Process for the isolation of fermentation products

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB08609776A Expired GB2175590B (en) 1985-04-22 1986-04-22 Process for the isolation of fermentation products

Country Status (11)

Country Link
EP (1) EP0201239B1 (en)
JP (1) JPS61282097A (en)
AT (1) ATE47426T1 (en)
AU (1) AU585006B2 (en)
DE (1) DE3666459D1 (en)
DK (1) DK184486A (en)
ES (1) ES8706828A1 (en)
FI (1) FI86079C (en)
GB (2) GB8510219D0 (en)
SG (1) SG87291G (en)
ZA (1) ZA863005B (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3689020T2 (en) * 1985-08-02 1994-04-07 Merck & Co Inc Fermentation yield of expressed protein.
JPS62215393A (en) * 1986-01-13 1987-09-22 ジエネツクス・コ−ポレ−シヨン Production of human serum albumin in batillus
GB8613388D0 (en) * 1986-06-03 1986-07-09 Delta Biotechnology Ltd Induction of galactose regulated gene expression in yeast
GB8615701D0 (en) * 1986-06-27 1986-08-06 Delta Biotechnology Ltd Stable gene integration vector
GB8620926D0 (en) * 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
AU613030B2 (en) * 1987-04-09 1991-07-25 Novozymes Delta Limited Yeast vector
GB8725529D0 (en) * 1987-10-30 1987-12-02 Delta Biotechnology Ltd Polypeptides
JPH01215289A (en) * 1988-02-22 1989-08-29 Toa Nenryo Kogyo Kk Production of normal human serum albumin a through gene recombination
FR2649991B2 (en) 1988-08-05 1994-03-04 Rhone Poulenc Sante USE OF STABLE DERIVATIVES OF PLASMID PKD1 FOR THE EXPRESSION AND SECRETION OF HETEROLOGOUS PROTEINS IN YEASTS OF THE GENUS KLUYVEROMYCES
US5260202A (en) * 1988-09-07 1993-11-09 Delta Biotechnology Limited Fermentation method
GB8905674D0 (en) * 1989-03-13 1989-04-26 Imperial College Dna construct and modified yeast
US5766883A (en) * 1989-04-29 1998-06-16 Delta Biotechnology Limited Polypeptides
US5993805A (en) * 1991-04-10 1999-11-30 Quadrant Healthcare (Uk) Limited Spray-dried microparticles and their use as therapeutic vehicles
GB9107628D0 (en) 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
FR2686899B1 (en) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa NOVEL BIOLOGICALLY ACTIVE POLYPEPTIDES, THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM.
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
EP2298355A3 (en) 2000-04-12 2011-06-29 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20080194481A1 (en) 2001-12-21 2008-08-14 Human Genome Sciences, Inc. Albumin Fusion Proteins
AU2002364587A1 (en) 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
PT1463751E (en) 2001-12-21 2013-08-26 Human Genome Sciences Inc Albumin fusion proteins
CA2485064A1 (en) 2002-02-07 2003-08-14 Delta Biotechnology Limited Albumin-fused kunitz domain peptides
WO2006034455A2 (en) 2004-09-23 2006-03-30 Vasgene Therapeutics, Inc. Polipeptide compounds for inhibiting angiogenesis and tumor growth
JP5256199B2 (en) 2006-08-07 2013-08-07 テヴァ バイオファーマシューティカルズ ユーエスエー，インコーポレーティッド Albumin-insulin fusion protein
CN102895797B (en) 2007-01-15 2016-01-20 厄普弗朗特色谱公司 Bio-fuel and protein is produced from raw material
EP1964923A1 (en) 2007-02-27 2008-09-03 Alain André Guy Vertès Multiplex fermentation process
DE102008008150A1 (en) * 2008-02-08 2009-08-13 Krones Ag Method for the extraction of ethanol from biomass, comprises dry grinding the biomass in a wet malt mill, mashing, fermenting and then distillating the biomass
US9499605B2 (en) 2011-03-03 2016-11-22 Zymeworks Inc. Multivalent heteromultimer scaffold design and constructs
JP6498601B2 (en) 2012-07-13 2019-04-10 ザイムワークス，インコーポレイテッド Multivalent heteromultimeric scaffold designs and constructs

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0079739A3 (en) * 1981-11-12 1984-08-08 The Upjohn Company Albumin-based nucleotides, their replication and use, and plasmids for use therein
GB8334261D0 (en) * 1983-12-22 1984-02-01 Bass Plc Fermentation processes

Also Published As

Publication number Publication date
JPS61282097A (en) 1986-12-12
GB2175590B (en) 1988-11-09
FI86079C (en) 1992-07-10
ATE47426T1 (en) 1989-11-15
ES554234A0 (en) 1987-07-01
EP0201239A1 (en) 1986-11-12
GB8609776D0 (en) 1986-05-29
EP0201239B1 (en) 1989-10-18
DE3666459D1 (en) 1989-11-23
SG87291G (en) 1992-02-14
AU5644886A (en) 1987-11-05
ZA863005B (en) 1986-12-30
ES8706828A1 (en) 1987-07-01
FI86079B (en) 1992-03-31
AU585006B2 (en) 1989-06-08
DK184486D0 (en) 1986-04-22
DK184486A (en) 1986-10-23
GB2175590A (en) 1986-12-03
FI861696A (en) 1986-10-23
FI861696A0 (en) 1986-04-22

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