GB2594339B - Enzymes with RUVC domains - Google Patents

Enzymes with RUVC domains Download PDF

Info

Publication number
GB2594339B
GB2594339B GB2010770.2A GB202010770A GB2594339B GB 2594339 B GB2594339 B GB 2594339B GB 202010770 A GB202010770 A GB 202010770A GB 2594339 B GB2594339 B GB 2594339B
Authority
GB
United Kingdom
Prior art keywords
enzymes
ruvc domains
ruvc
domains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB2010770.2A
Other versions
GB202010770D0 (en
GB2594339A (en
Inventor
Thomas Brian
Brown Christopher
Kantor Rose
Devoto Audra
Butterfield Cristina
Alexander Lisa
S A Goltsman Daniela
Liu Jason
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Metagenomi Inc
Original Assignee
Metagenomi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2020/018432 external-priority patent/WO2020168291A1/en
Application filed by Metagenomi Inc filed Critical Metagenomi Inc
Priority to GB2219441.9A priority Critical patent/GB2617659B/en
Publication of GB202010770D0 publication Critical patent/GB202010770D0/en
Publication of GB2594339A publication Critical patent/GB2594339A/en
Application granted granted Critical
Publication of GB2594339B publication Critical patent/GB2594339B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB2010770.2A 2020-02-14 2020-07-13 Enzymes with RUVC domains Active GB2594339B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2219441.9A GB2617659B (en) 2020-02-14 2020-07-13 Enzymes with RuvC domains

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2020/018432 WO2020168291A1 (en) 2019-02-14 2020-02-14 Enzymes with ruvc domains
US202063022320P 2020-05-08 2020-05-08

Publications (3)

Publication Number Publication Date
GB202010770D0 GB202010770D0 (en) 2020-08-26
GB2594339A GB2594339A (en) 2021-10-27
GB2594339B true GB2594339B (en) 2023-02-08

Family

ID=72140085

Family Applications (2)

Application Number Title Priority Date Filing Date
GB2219441.9A Active GB2617659B (en) 2020-02-14 2020-07-13 Enzymes with RuvC domains
GB2010770.2A Active GB2594339B (en) 2020-02-14 2020-07-13 Enzymes with RUVC domains

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB2219441.9A Active GB2617659B (en) 2020-02-14 2020-07-13 Enzymes with RuvC domains

Country Status (1)

Country Link
GB (2) GB2617659B (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190249200A1 (en) * 2018-02-15 2019-08-15 Sigma-Aldrich Co., Llc Engineered cas9 systems for eukaryotic genome modification
WO2020168291A1 (en) * 2019-02-14 2020-08-20 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230156800A (en) * 2015-03-03 2023-11-14 더 제너럴 하스피탈 코포레이션 Engineered crispr-cas9 nucleases with altered pam specificity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190249200A1 (en) * 2018-02-15 2019-08-15 Sigma-Aldrich Co., Llc Engineered cas9 systems for eukaryotic genome modification
WO2020168291A1 (en) * 2019-02-14 2020-08-20 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Frontiers in Plant Science, Vol. 7, 2016, Ding et. al., "Recent Advances in Genome Editing Using CRISPR/Cas9" *
Molecular cell, vol. 60, 2015, Shmakov et. al., Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems, pp. 385-397. *
Nature reviews microbiology, vol. 18, 2020, Makarova et. al., "Evolutionary classification of CRISPRCas systems: a burst of class 2 and derived variants", pp. 67-83 *
Science, vol. 337, 2012, Jinek at. al., A Programmable Dual-RNA Guided DNA Endonuclease in Adaptive Bacterial Immunity, pp. 816-821. *

Also Published As

Publication number Publication date
GB202010770D0 (en) 2020-08-26
GB202219441D0 (en) 2023-02-01
GB2617659B (en) 2024-05-01
GB2594339A (en) 2021-10-27
GB2617659A (en) 2023-10-18

Similar Documents

Publication Publication Date Title
GB202217378D0 (en) Enzymes with ruvc domains
CA3241703A1 (en) Enzymes with ruvc domains
EP4069281A4 (en) Novel enzymes
EP3924477A4 (en) Enzymes with ruvc domains
EP3882345A4 (en) Crispr-cas12j enzyme and system
EP3870697A4 (en) Engineered enzymes
IL289202A (en) Uridine diphosphate-dependent glycosyltransferase enzyme
IL281171A (en) Cellulose-synthase-like enzymes and uses thereof
EP3843774A4 (en) Cardiac cell reprogramming with myocardin and ascl1
EP3846938A4 (en) Immobilized enzymes and microsomes on magnetic scaffolds
IL279883A (en) Engineered galactose oxidase variant enzymes
EP3802827A4 (en) Modified rna agents with reduced off-target effect
EP4165177A4 (en) Enzymes with ruvc domains
EP3954774A4 (en) Rna site-directed editing using artificially constructed rna editing enzymes and related uses
EP3840582A4 (en) Modification of protein glycosylation in microorganisms
EP3821026A4 (en) Engineered phosphopentomutase variant enzymes
EP3938400A4 (en) Cd22 antibodies and methods of using the same
EP4093863A4 (en) Crispr-cas enzymes with enhanced on-target activity
EP4074837A4 (en) Modified daao enzyme and application thereof
GB201917688D0 (en) SHC enzymes and enzyme variants
GB2617659B (en) Enzymes with RuvC domains
EP3827078A4 (en) Encapsulated microorganisms and methods of using same
EP3775255A4 (en) Metabolic assay for bacterial growth and gram typing
EP3917970A4 (en) Engineered microorganisms and methods of making and using same
EP3864149A4 (en) Protease enzyme variants and uses thereof

Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20220317 AND 20220323