GB1306751A - Lactase enzyme production - Google Patents

Lactase enzyme production

Info

Publication number
GB1306751A
GB1306751A GB1306751DA GB1306751A GB 1306751 A GB1306751 A GB 1306751A GB 1306751D A GB1306751D A GB 1306751DA GB 1306751 A GB1306751 A GB 1306751A
Authority
GB
United Kingdom
Prior art keywords
lactase
active
acid
component
lactase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter International Inc
Original Assignee
Baxter Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Laboratories Inc filed Critical Baxter Laboratories Inc
Publication of GB1306751A publication Critical patent/GB1306751A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1306751 Lactase BAXTER LABORATORIES Inc 26 Jan 1971 3126/71 Heading C3H A method for the production of an acid-active, acid-stable lactase enzyme preparation comprises growing a culture of Aspergillus niger under aerobic fermentation conditions, extracting the growth product with water, slurrying the extract with a hydrated aluminium silicate adsorbent at a pH of from 3 to 6 to adsorb an active lactase component, separating the precipitate and releasing the active lactase component therefrom by adjusting the pH to 7 to 8 with an aqueous alkaline reagent. The silicate may be bentonite or kaolin.
GB1306751D 1971-01-26 1971-01-26 Lactase enzyme production Expired GB1306751A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB312671 1971-01-26

Publications (1)

Publication Number Publication Date
GB1306751A true GB1306751A (en) 1973-02-14

Family

ID=9752414

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1306751D Expired GB1306751A (en) 1971-01-26 1971-01-26 Lactase enzyme production

Country Status (1)

Country Link
GB (1) GB1306751A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002081673A1 (en) * 2001-04-04 2002-10-17 Dsm Ip Assets B.V. Purified lactase
WO2015132349A1 (en) * 2014-03-05 2015-09-11 Dsm Ip Assets B.V. Liquid lactase compositions
WO2017106264A1 (en) * 2015-12-14 2017-06-22 Dupont Nutrition Biosciences Aps Method for producing a clean lactase
CN116004398A (en) * 2022-10-12 2023-04-25 宁波希诺亚海洋生物科技有限公司 Aspergillus niger strain capable of expressing lactase and protease and application thereof

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002081673A1 (en) * 2001-04-04 2002-10-17 Dsm Ip Assets B.V. Purified lactase
EP2280065A1 (en) * 2001-04-04 2011-02-02 DSM IP Assets B.V. Purified lactase
US7955831B2 (en) 2001-04-04 2011-06-07 Dsm Ip Assets B.V. Purified lactase
US9149048B2 (en) 2001-04-04 2015-10-06 Dsm Ip Assets B.V. Purified lactase
US9763460B2 (en) 2001-04-04 2017-09-19 Dsm Ip Assets B.V. Purified lactase
WO2015132349A1 (en) * 2014-03-05 2015-09-11 Dsm Ip Assets B.V. Liquid lactase compositions
US10201178B2 (en) 2014-03-05 2019-02-12 Dsm Ip Assets B.V. Stable liquid lactase compositions
WO2017106264A1 (en) * 2015-12-14 2017-06-22 Dupont Nutrition Biosciences Aps Method for producing a clean lactase
CN116004398A (en) * 2022-10-12 2023-04-25 宁波希诺亚海洋生物科技有限公司 Aspergillus niger strain capable of expressing lactase and protease and application thereof

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Legal Events

Date Code Title Description
PS Patent sealed
PLE Entries relating assignments, transmissions, licences in the register of patents