ES2192960B1 - Nuevo procedimiento de obtencion de acido hialuronico. - Google Patents

Nuevo procedimiento de obtencion de acido hialuronico.

Info

Publication number
ES2192960B1
ES2192960B1 ES200102542A ES200102542A ES2192960B1 ES 2192960 B1 ES2192960 B1 ES 2192960B1 ES 200102542 A ES200102542 A ES 200102542A ES 200102542 A ES200102542 A ES 200102542A ES 2192960 B1 ES2192960 B1 ES 2192960B1
Authority
ES
Spain
Prior art keywords
obtaining hyaluronic
new procedure
hyaluronic acid
hydroalcoholic
recovery
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200102542A
Other languages
English (en)
Other versions
ES2192960A1 (es
Inventor
Miguel Anxo Murado Garcia
Maria Ignacia Montemayor Castroviejo
Marta Lopez Cabo
Maria Del Pilar Gonzalez Fernandez
Laura Pastoriza Enriquez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200102542A priority Critical patent/ES2192960B1/es
Publication of ES2192960A1 publication Critical patent/ES2192960A1/es
Application granted granted Critical
Publication of ES2192960B1 publication Critical patent/ES2192960B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Cosmetics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Nuevo procedimiento de obtención de ácido hialurónico. El procedimiento de obtención de ácido hialurónico (AH) a partir del humor vítreo de peces, entre otros materiales biológicos de partida, descrita en esta invención se basa en la sucesión de: 1) una etapa de electrodeposición proteica, previa o simultánea a un proceso de diafiltración con recirculación total, 2) una recuperación selectiva en solución hidroalcohólica de sedimentos impuros obtenidos por precipitación alcohólica exhaustiva, 3) un tratamiento alcalino en solución hidroalcohólica y condiciones controladas de alcalinidad, temperatura, proporción de etanol y tiempo, que precipita el AH y solubiliza proteínas, y 4) la recuperación del AH por resuspensión del precipitado alcalino en fosfato monosódico hidroalcohólico, que disuelve el AH, neutraliza el extracto y deja en el sedimento los restos proteicos insolubles. Se obtienen de este modo preparados de AH de alta pureza (superior al 99,5 %), aptos para aplicaciones en clínica ycosmética.
ES200102542A 2001-11-16 2001-11-16 Nuevo procedimiento de obtencion de acido hialuronico. Expired - Fee Related ES2192960B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200102542A ES2192960B1 (es) 2001-11-16 2001-11-16 Nuevo procedimiento de obtencion de acido hialuronico.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200102542A ES2192960B1 (es) 2001-11-16 2001-11-16 Nuevo procedimiento de obtencion de acido hialuronico.

Publications (2)

Publication Number Publication Date
ES2192960A1 ES2192960A1 (es) 2003-10-16
ES2192960B1 true ES2192960B1 (es) 2005-03-01

Family

ID=29724693

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200102542A Expired - Fee Related ES2192960B1 (es) 2001-11-16 2001-11-16 Nuevo procedimiento de obtencion de acido hialuronico.

Country Status (1)

Country Link
ES (1) ES2192960B1 (es)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59166504A (ja) * 1983-03-10 1984-09-19 Ichimaru Fuarukosu Kk ヒアルロン酸の製造法
AU593556B2 (en) * 1985-05-09 1990-02-15 David Cullis-Hill High purity hyaluronic acid from synovial fluid
JP2893451B2 (ja) * 1988-08-09 1999-05-24 昭和産業株式会社 高分子量のヒアルロン酸を製造する方法
RU2074196C1 (ru) * 1993-08-23 1997-02-27 Научно-производственная фирма "Нарт" Способ получения гиалуроновой кислоты
CH692919A5 (fr) * 1999-01-28 2002-12-13 Trb Chemedica S A Procédé de purification de l'acide hyaluronique à poids moléculaire élevé.
RU2157381C1 (ru) * 1999-03-01 2000-10-10 Общество с ограниченной ответственностью "Научно-производственное предприятие "Тульская индустрия ЛТД" Способ получения гиалуроновой кислоты

Also Published As

Publication number Publication date
ES2192960A1 (es) 2003-10-16

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