ES2056028B1 - PROCEDURE FOR THE SELECTION OF OLIGONUCLEOTIDES FOR THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOMES. - Google Patents

PROCEDURE FOR THE SELECTION OF OLIGONUCLEOTIDES FOR THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOMES.

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Publication number
ES2056028B1
ES2056028B1 ES09300320A ES9300320A ES2056028B1 ES 2056028 B1 ES2056028 B1 ES 2056028B1 ES 09300320 A ES09300320 A ES 09300320A ES 9300320 A ES9300320 A ES 9300320A ES 2056028 B1 ES2056028 B1 ES 2056028B1
Authority
ES
Spain
Prior art keywords
genomes
oligonucleotides
procedure
selection
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES09300320A
Other languages
Spanish (es)
Other versions
ES2056028A1 (en
Inventor
Blazquez Joaquin Dopazo
Castello Francisco Sobrino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority to ES09300320A priority Critical patent/ES2056028B1/en
Publication of ES2056028A1 publication Critical patent/ES2056028A1/en
Application granted granted Critical
Publication of ES2056028B1 publication Critical patent/ES2056028B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

PROCEDIMIENTO PARA LA SELECCION DE OLIGONUCLEOTIDOS PARA LA AMPLIFICACION ESPECIFICA DE GENOMAS ALTAMENTE VARIABLES. EL PROCEDIMIENTO OBJETO DE LA PRESENTE INVENCION CONSISTE EN QUE MEDIANTE UNA SUCESION ESPECIFICA DE OPERACIONES SE LOCALIZAN Y SELECCIONAN REGIONES IDONEAS QUE PERMITAN LA AMPLIFICACION ESPECIFICA DE GRUPOS DE GENOMAS ALTAMENTE VARIABLES MEDIANTE EL USO DE POLIMERASAS TERMOESTABLES (RCP). EL PROCEDIMIENTO SE BASA EN UNA COMBINACION DE CRITERIOS TERMODINAMICOS Y CONSIDERACIONES SOBRE LA VARIABILIDAD DE LOS GENOMAS A COMPARAR. DE ESTA MANERA SE SELECCIONAN OLIGONUCLEOTIDOS ALTAMENTE CONSERVADOS ENTRE EL GRUPO DE GENOMAS QUE SE PRETENDE AMPLIFICAR Y QUE PRESENTAN, A SU VEZ, EL MAYOR NUMERO POSIBLE DE DIFERENCIAS DE NUCLEOTIDO FRENTE A OTROS GENOMAS ESPECIFICOS. LA APLICACION DE LA INVENCION AL ANALISIS DE SECUENCIAS GENOMICAS DEL VIRUS DE LA FIEBRE AFTOSA HA PERMITIDO LA SELECCION DE OLIGONUCLEOTIDOS QUE HACEN POSIBLE LA DETECCION ESPECIFICA DE ESTE GENOMA PATOGENO MEDIANTE LATECNICA DE RCP.PROCEDURE FOR THE SELECTION OF OLIGONUCLEOTIDES FOR THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOMES. THE OBJECT PROCEDURE OF THE PRESENT INVENTION CONSISTS THAT THROUGH A SPECIFIC SUCCESSION OF OPERATIONS, SUITABLE REGIONS ARE LOCATED AND SELECTED THAT ALLOW THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOME GROUPS (USING THERMOESTABLE POLYMERES). THE PROCEDURE IS BASED ON A COMBINATION OF THERMODYNAMIC CRITERIA AND CONSIDERATIONS ON THE VARIABILITY OF THE GENOMES TO COMPARE. IN THIS WAY, HIGHLY PRESERVED OLIGONUCLEOTIDES ARE SELECTED AMONG THE GROUP OF GENOMES THAT ARE INTENDED TO AMPLIFY AND WHICH, IN turn, HAVE THE GREATEST POSSIBLE NUCLEOTIDE DIFFERENCES AGAINST OTHER SPECIFIC GENOMES. THE APPLICATION OF THE INVENTION TO THE ANALYSIS OF GENOMIC SEQUENCES OF FOOT-AND-MOUTH DISEASE VIRUS HAS ALLOWED THE SELECTION OF OLIGONUCLEOTIDES THAT MAKE POSSIBLE THE SPECIFIC DETECTION OF THIS PATHOGENIC GENOME THROUGH LATECNICA RCP.

ES09300320A 1993-02-18 1993-02-18 PROCEDURE FOR THE SELECTION OF OLIGONUCLEOTIDES FOR THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOMES. Expired - Fee Related ES2056028B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09300320A ES2056028B1 (en) 1993-02-18 1993-02-18 PROCEDURE FOR THE SELECTION OF OLIGONUCLEOTIDES FOR THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOMES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09300320A ES2056028B1 (en) 1993-02-18 1993-02-18 PROCEDURE FOR THE SELECTION OF OLIGONUCLEOTIDES FOR THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOMES.

Publications (2)

Publication Number Publication Date
ES2056028A1 ES2056028A1 (en) 1994-09-16
ES2056028B1 true ES2056028B1 (en) 1995-04-01

Family

ID=8280828

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09300320A Expired - Fee Related ES2056028B1 (en) 1993-02-18 1993-02-18 PROCEDURE FOR THE SELECTION OF OLIGONUCLEOTIDES FOR THE SPECIFIC AMPLIFICATION OF HIGHLY VARIABLE GENOMES.

Country Status (1)

Country Link
ES (1) ES2056028B1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8606719D0 (en) * 1986-03-19 1986-04-23 Lister Preventive Med Genetic probes
GB8716367D0 (en) * 1987-07-10 1987-08-19 Reeders S T Genetic identification
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
EP0511750A1 (en) * 1991-04-09 1992-11-04 The Regents Of The University Of California Repeat sequence chromosome specific nucleic acid probes

Also Published As

Publication number Publication date
ES2056028A1 (en) 1994-09-16

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Effective date: 20180806