EP2385103A3 - Variant Hypocrea jecorina CBH1 - Google Patents

Variant Hypocrea jecorina CBH1 Download PDF

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Publication number
EP2385103A3
EP2385103A3 EP11158906A EP11158906A EP2385103A3 EP 2385103 A3 EP2385103 A3 EP 2385103A3 EP 11158906 A EP11158906 A EP 11158906A EP 11158906 A EP11158906 A EP 11158906A EP 2385103 A3 EP2385103 A3 EP 2385103A3
Authority
EP
European Patent Office
Prior art keywords
variant
jecorina cbh1
hypocrea jecorina
cbh1
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11158906A
Other languages
German (de)
French (fr)
Other versions
EP2385103A2 (en
Inventor
Edmund A. Larenas
Frits Goedegebuur
Peter Gualfetti
Colin Mitchinson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of EP2385103A2 publication Critical patent/EP2385103A2/en
Publication of EP2385103A3 publication Critical patent/EP2385103A3/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)

Abstract

Disclosed are variants of Humicola grisea Ce17A (CHB1.1), H.jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
EP11158906A 2003-04-01 2004-03-26 Variant Hypocrea jecorina CBH1 Withdrawn EP2385103A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45973403P 2003-04-01 2003-04-01
EP04785732.1A EP1627050B1 (en) 2003-04-01 2004-03-26 Variant humicola grisea cbh1.1

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
EP04785732.1 Division 2004-03-26

Publications (2)

Publication Number Publication Date
EP2385103A2 EP2385103A2 (en) 2011-11-09
EP2385103A3 true EP2385103A3 (en) 2012-02-22

Family

ID=33551336

Family Applications (3)

Application Number Title Priority Date Filing Date
EP11158906A Withdrawn EP2385103A3 (en) 2003-04-01 2004-03-26 Variant Hypocrea jecorina CBH1
EP11158910A Withdrawn EP2385104A1 (en) 2003-04-01 2004-03-26 Variant Scytalidium thermophilium CBH1
EP04785732.1A Expired - Lifetime EP1627050B1 (en) 2003-04-01 2004-03-26 Variant humicola grisea cbh1.1

Family Applications After (2)

Application Number Title Priority Date Filing Date
EP11158910A Withdrawn EP2385104A1 (en) 2003-04-01 2004-03-26 Variant Scytalidium thermophilium CBH1
EP04785732.1A Expired - Lifetime EP1627050B1 (en) 2003-04-01 2004-03-26 Variant humicola grisea cbh1.1

Country Status (10)

Country Link
US (8) US7459299B2 (en)
EP (3) EP2385103A3 (en)
JP (4) JP5427342B2 (en)
CN (2) CN102517360A (en)
CA (2) CA2520636C (en)
DK (1) DK1627050T3 (en)
ES (1) ES2430825T3 (en)
PL (1) PL1627050T3 (en)
SI (1) SI1627050T1 (en)
WO (1) WO2005001065A2 (en)

Families Citing this family (145)

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