EP1514600A1 - Mechanical piston pipette - Google Patents

Mechanical piston pipette Download PDF

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Publication number
EP1514600A1
EP1514600A1 EP03405604A EP03405604A EP1514600A1 EP 1514600 A1 EP1514600 A1 EP 1514600A1 EP 03405604 A EP03405604 A EP 03405604A EP 03405604 A EP03405604 A EP 03405604A EP 1514600 A1 EP1514600 A1 EP 1514600A1
Authority
EP
European Patent Office
Prior art keywords
pipette
calibration
volume
piston
rotation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP03405604A
Other languages
German (de)
French (fr)
Other versions
EP1514600B2 (en
EP1514600B1 (en
Inventor
Jean-Pierre Uldry
Sylvain Christen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Socorex Isba SA
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Socorex Isba SA
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Application filed by Socorex Isba SA filed Critical Socorex Isba SA
Priority to AT03405604T priority Critical patent/ATE365075T1/en
Priority to EP03405604A priority patent/EP1514600B2/en
Priority to DE60314516T priority patent/DE60314516T3/en
Priority to ES03405604T priority patent/ES2289252T5/en
Priority to US10/916,359 priority patent/US7204163B2/en
Publication of EP1514600A1 publication Critical patent/EP1514600A1/en
Publication of EP1514600B1 publication Critical patent/EP1514600B1/en
Application granted granted Critical
Publication of EP1514600B2 publication Critical patent/EP1514600B2/en
Anticipated expiration legal-status Critical
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/148—Specific details about calibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/026—Drum counters

Definitions

  • the present invention relates to a piston pipette mechanical device comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged so that it can be with replaceable tip and ejector endpiece, movable along the cylinder to eject a tip of the end of the cylinder, the said pipette body accommodating an ejection actuating device and a piston actuating device performing a stroke determined in longitudinal translation between a position of low stop and a high stop position to perform aspiration and dosing of liquids, with means of modification of the said race.
  • piston pipettes are known in the state of the art. We distinguish pipettes mechanical devices whose mechanism is entirely actuated by the hand of the user. When these pipettes have a way volume display, this one can be analog, digital and mechanical, or electronic. There are also many types of piston pipettes powered by an engine electrical equipment and whose display devices or adjustment or calibration devices are also performed by electromechanical or electronic means.
  • the present invention relates exclusively to pipettes to mechanical piston, with fixed or adjustable volume. When the volume is adjustable, the invention relates exclusively to pipettes whose volume display itself is mechanical.
  • volume-adjustable pipettes For highly repetitive laboratory operations for analysis, for example in test kits, it is preferred to use fixed volume pipettes.
  • volume-adjustable pipettes For small operations repetitive or in research laboratories, generally prefers to use volume-adjustable pipettes, allowing a wider range of use. Even if we do not takes into consideration that the range of volumes 1 ⁇ l to 1000 ⁇ l, which concerns the pipettes commonly called micro-pipettes, whereas pipettes called “macro-pipettes" cover the higher volume ranges, a full range of micro-pipettes usually represents more than 20 different objects present in a manufacturer's catalog. A great concern for manufacturers is therefore reducing the total number of parts different entering the constitution of the different micro-pipettes of the same series. He is particularly desirable to be able to use as many people as possible of common parts to make volume micro-pipettes fixed and variable volume, in the volume ranges comparable.
  • a pipette with adjustable volume comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged so as to be provided with a replaceable tip, and a tip ejector device, movable along the cylinder to eject a mouthpiece the end of the cylinder, the so-called pipette body welcoming an ejection actuating device and a device piston actuator performing a determined stroke in longitudinal translation between a low stop position and a high stop position to perform the suction of liquids, with means for modifying said stroke, means for adjusting the pipetted volume, display means pipetting volume and calibration means, by the fact that said adjustment means, said display means and the said calibration means cooperate in a device for setting and calibration in which, during an operation of calibration, the said display means are adjusted so to match the displayed volume with the actual pipetted volume and that said adjustment and calibration device is housed inside the pipette body.
  • the adjustable volume pipette body shown in the figure 1 comprises an outer sleeve 14 which itself consists of a longitudinal portion 14a, substantially cylindrical but with a slight ergonomic bulge to fit the palm of the hand, with a substantially elliptical cross-section, of which the upper part is extended laterally by a stick 14b in a comma form intended to rest on the flank of the index of the user's hand.
  • the outer sleeve 14 accommodates an inner sleeve 13 which fits into the sleeve outside 14 on the same side as the butt 14b.
  • the sleeve interior 13 has an inner wall portion cylindrical with an arcuate section, which forms with the inner surface of the opposite zone of the outer sleeve 14 a cylindrical housing of circular section.
  • the sleeve interior 13 is thick enough to accommodate the tip ejection device, which includes a button ejector 10 surmounted by a protective cap 9.
  • the button ejector is extended by an ejector rod 18 on which are threaded an ejection spring 12 and a sleeve 11.
  • the inner sleeve 13 also houses a pinion tube 19 comprising a plurality of rotary gears, for example three pinions 19a, 19b, 19c, rotating about an axis parallel to the longitudinal axis of the pipette and cooperating with the setting and calibration device as it will be described later.
  • the top of the sleeve 13 is extended laterally by a ring 13a, coaxial with the housing cylindrical, which caps the upper opening of the sleeve 14.
  • a metering knob 3 protrudes from the top of the ring 13a.
  • the metering button 3 is intended to be embedded in the body of pipette by the user's thumb during operations pipetting, namely aspiration of liquid into the mouthpiece Disposable and ejection of this liquid.
  • the doser button 3 is pushed upwards by the pipetting spring 7, so that perform alternative translational movements according to the longitudinal axis of the pipette body.
  • the top of the button metering device 3 is plugged by a cap 1.
  • the cap 1 is easily removed by the user by means of a fingernail. he can be realized in different colors to facilitate visual identification of the pipette.
  • the metering knob 3 is threaded internally. He receives a adjusting screw 4 threaded externally, so that the rotation of the metering knob 3 causes the adjusting screw 4 longitudinal translation, when it is blocked itself in rotation as described below.
  • the adjusting screw 4 is shown in its extreme position lower.
  • the upper part of the adjusting screw 4 is recessed and receives a calibration key 2 which can slide in longitudinal translation in the recess of the screw of setting 4, but which is locked in rotation. To do this, the recess of the adjusting screw 4 and the calibration key can both be square.
  • the key to calibration is threaded into a ring 5.
  • the middle part of the adjusting screw 4 carries a bulge 4a, come from one piece with the body of the screw 4, on which rests an over-stroke spring 4b.
  • the other extremity of the overtravel spring 4b rests on an overtravel stop 4c fitted on and secured with the body of the screw setting 4.
  • the adjustment and calibration device is designated in its set by 8. It includes a calibration disk 8a.
  • the calibration disk 8a comprises an upper barrel 8b surrounding the body of the adjusting screw 4, which slides therein longitudinally, and a lower flange 8c in diameter greater than that of the barrel 8b.
  • the calibration disk 8a rests on the bottom 14c of the outer sleeve 14 and thus limits the stroke of the screw 4, when the lower face of the stop 4c gets in touch with him.
  • An 8d adjustment tube is based on 8h circular horizontal plate formed between flange 8c and the barrel 8b.
  • the adjustment tube 8d and the disc of calibration 8a are coupled by a dog clutch system coupling C, consisting of two toothed crowns being face, respectively formed on the 8h replat of the disk of calibration and on the lower end face of the tube 8d setting. Teeth are low in height and disengage easily.
  • the outer sleeve 14 houses, at the disc level 8a, a locking device consisting of a shoe 15 provided with an elastic clip 16. In position locked, this pad 15 prevents the rotation of the disk from calibration 8a.
  • the pad is manoeuvrable from the outside, by a hole of the sleeve 14, by any object having a fine point.
  • the calibration disk 8a is made integral in rotation the body of the adjusting screw 4 by means of ribs / grooves longitudinal conjugates.
  • the top of the 8d adjustment tube surrounds the bottom of the metering knob 3.
  • the 8d adjustment tube and the metering button 3 are also secured in rotation by ribs / conjugate grooves.
  • the adjustment tube has a lower part of outer diameter slightly decreased, forming a protrusion horizontal circular.
  • the 8d adjustment tube carries other share on this lower part a plurality of rings of counting, for example three rings 8e, 8f, 8g bearing on their periphery of the digits from 0 to 9, providing an indication digital volume of liquid pipetted.
  • the outer sleeve 14 has on its side opposite the butt 14b and at the height of the counting rings a window transparent 6 to read the volume indication.
  • the projection of the adjustment tube 8d facing the rings of counting comprises several teeth causing the pinion 19a.
  • the counting rings have a continuous toothing on the upper frontal face but only one indentation on the opposite end face, so that the rotation of the disc of setting 8d drives the first pinion, which in turn drives the first 8th disc which, for example after a lap complete, drives the second pinion 19b rotating a digit the second counting ring, and so on. This mechanism is in itself known.
  • the locking shoe 15 is in the locked position, preventing the rotation of the calibration disk 8a.
  • the disc calibration locks the rotation of the adjusting screw 4, which it itself is integral in rotation with the calibration key 2.
  • the user rotates the metering knob 3, which, by its internal thread, drives the adjusting screw 4.
  • This displacement in longitudinal translation operates on the stroke adjustment extending between the bottom of the stop of race 4c and the top of the calibration disc 8a.
  • This course determines itself the volume of liquid, since the screw of setting 4 is connected to the piston of the pipette by its end 4d in the form of a spherical head constituting a detachable connection.
  • the rotation of the metering knob 3 results in rotating the adjustment tube 8d and, via the gear tube 19, the 8th, 8f, 8g meter disks, as discussed above.
  • the claw system C is uncoupled, the tube 8d turns on the calibration disk 8a, the teeth of the segments teeth ripping one above the other.
  • the stroke defined by the distance between the overtravel stop 4c and the disc of 8a does not, in the majority of cases, correspond to displayed volume.
  • a calibration is necessary at the factory. The subsequent user may also wish periodically, for example for specific liquids or again after dismantling, cleaning and sterilization, recalibrate. An operation of calibration is carried out as follows:
  • the volume dispensed by the pipette can be 545 ⁇ l while the meter displays 210 ⁇ l.
  • the operation of calibration is to bring the value of the display to 545 ⁇ l corresponding to the actual volume, without changing the stroke.
  • the locking shoe 15 is unlocked, thus freeing the rotation of the disc from calibration 8a.
  • the cap 1 is removed to access the screw 2. This can be operated either by a coin or a screwdriver, either, after having partially slid outward, can be operated at the hand.
  • the rotation of the calibration key leads to turning the adjusting screw 4, which itself leads to rotation the calibration disc. While turning the key the user is careful not to touch the doser button 3 so as not to change the relative position button 3 / screw 4.
  • the calibration disc drives the tube of 8d setting in rotation, through the system of jaws C.
  • the rotation of the adjustment tube causes the rotation pinions of the pinion tube 19 and therefore the rotation of the counting rings.
  • the key is turned until the measured value, in this example 545 ⁇ l, is displayed.
  • the pad 15 is pushed back into the locking position to lock in angular position the calibration disc 8a and the adjusting screw 4.
  • the calibration key 2 is pushed back into its housing and the hood is replaced.
  • the value of the counter device being modeled on the stroke, the subsequent volume adjustments of the pipette are made simply by rotating the metering knob 3, which modifies this times the stroke by displacement in translation of the screw of adjustment 4 without moving in rotation thereof.
  • the fixed volume pipette body shown in Figure 2 comprises an outer sleeve 14 which itself consists of a longitudinal portion 14a, substantially cylindrical but with a slight ergonomic bulge to fit the palm of the hand, with a substantially elliptical cross-section, of which the upper part is extended laterally by a stick 14b in a comma form intended to rest on the flank of the index of the user's hand.
  • the outer sleeve 14 accommodates an inner sleeve 13 which fits into the sleeve outside 14 on the same side as the butt 14b.
  • the sleeve interior 13 has an inner wall portion cylindrical with an arcuate section, which forms with the inner surface of the opposite zone of the outer sleeve 14 a cylindrical housing of circular section.
  • the sleeve interior 13 is thick enough to accommodate the tip ejection device, which includes a button ejector 10 surmounted by a protective cap 9.
  • the button ejector is extended by an ejector rod 18 on which are threaded an ejection spring 12 and a sleeve 11.
  • the location of the pinion tube 19 described above remains empty.
  • the top of the sleeve 13 extends laterally by a ring 13a, coaxial with the cylindrical housing, which cap the upper opening of the sleeve 14.
  • a metering knob 3 protrudes from the top of the ring 13a.
  • the metering button 3 is intended to be embedded in the body of pipette by the user's thumb during operations pipetting, namely aspiration of liquid into the mouthpiece Disposable and ejection of this liquid.
  • the doser button 3 is pushed upwards by the pipetting spring 7, so that perform alternative translational movements according to the longitudinal axis of the pipette body.
  • the top of the button metering device 3 is plugged by a cap 1.
  • the cap 1 is easily removed by the user by means of a fingernail. he can be realized in different colors to facilitate visual identification of the pipette.
  • the metering knob 3 is threaded internally. He receives a adjusting screw 4 externally threaded.
  • the adjusting screw 4 is shown in its extreme position lower.
  • the upper part of the adjusting screw 4 is recessed and receives a calibration key 2 which can slide in longitudinal translation in the recess of the screw of setting 4, but which is locked in rotation. To do this, the recess of the adjusting screw 4 and the calibration key can both be square.
  • the key to calibration is threaded into a ring 5.
  • the middle part of the adjusting screw 4 carries a bulge 4a, come from one piece with the body of the screw 4, on which rests an over-stroke spring 4b.
  • the other extremity of the overtravel spring 4b rests on an overtravel stop 4c fitted on and secured with the body of the screw setting 4.
  • the calibration device is designated as a whole by 8 '. It includes a calibration disk 8a.
  • the record of calibration 8a comprises an upper barrel 8b surrounding the body of the adjusting screw 4, which slides there longitudinally, and a lower flange 8c in diameter greater than that of the barrel 8b.
  • the calibration disk 8a rests on the bottom 14c of the outer sleeve 14 and thus limits the stroke of the screw 4, when the lower face of the stop 4c gets in touch with him.
  • the calibration disk 8a can be identical to that shown in Figure 1, and bear a Circular toothed crown, formed on the plate 8h of the disc calibration; but, in the case of a fixed volume pipette, this crown has no function.
  • the outer sleeve 14 at the level of the calibration disk 8a a device for lock consisting of a shoe 15 provided with a clip 16. In the locked position, this pad 15 prevents the rotation of the calibration disk 8a.
  • the pad is manoeuvrable from the outside, through a hole in the sleeve 14, by any object having a fine point.
  • the calibration disk 8a is secured in rotation of the body of the adjusting screw 4 to means of ribs / longitudinal grooves conjugate.
  • the tube 8d has like the tube 8d described above a lower part of outer diameter slightly decreased, forming a horizontal circular projection. But the tube 8d 'does wears no counting rings.
  • the outer sleeve 14 has on its side opposite the 14b stock and at the height of the lower part of the tube 8d a opaque plate 6 'replacing window 6.
  • the stroke defined by the distance between the overtravel stop 4c and the disc of 8a does not, in the majority of cases, correspond to nominal volume.
  • a calibration is necessary at the factory. The subsequent user may also wish periodically, for example after disassembly operations, cleaning and sterilization, recalibrate.
  • a calibration operation is performed as follows:
  • the volume dispensed by the pipette can be 545 ⁇ l while the nominal value is 500 ⁇ l.
  • the operation calibration is to bring the value of the actual volume to the nominal value.
  • the locking shoe 15 is unlocked, thus freeing the rotation of the disc from calibration 8a.
  • the cap 1 is removed to access the screw 2.
  • This can be operated either by a screwdriver or a coin, that is, after having partially slid outward, can be operated at the hand.
  • the rotation of the calibration key leads to turning the adjusting screw 4, which itself leads to rotation the calibration disc.
  • the calibration disc slides under the tube 8d '.
  • the adjusting screw moves in longitudinal translation, modifying the stroke, and so the volume pipetted. After measuring this volume, the operation can be repeated until the measured value coincides with the nominal value or with another desired value by the user.
  • the pad 15 is pushed back into position lock in order to lock in the angular position the calibration disk 8a and the adjusting screw 4.
  • the key of calibration 2 is pushed back into its housing and the cap is replaced.
  • variable volume pipette and fixed volume pipette body use, in the same volume range, a large majority common rooms. These are identified by numbers identical reference numbers in figures 1 and 2.
  • the man of the profession will observe in particular that the same piece, the ring of calibration 8a, provides several functions, namely stop end of stroke, adjustment and / or calibration in the two types of pipette.
  • One essential piece, namely the tube 8d, respectively 8d ' has structures and different functions: the tube 8d serves both as a guide for metering button 3, housing spring 7 and provides a adjustment function as explained above.
  • the tube 8d which differs essentially from the tube 8d by the presence of ribs the outer blocking it in rotation, serves only as a guide and of housing. It will be noted that the tubes 8d and 8d 'have moreover identical dimensions.
  • the pipette body to adjustable volume also has a set of rings counting and drive gears described above, that are not present in the body of the volume pipette fixed.
  • the entire adjustment and calibration device is housed in the longitudinal pipette body, without increase volume of it, and the display, which is not hidden by the thumb, remains visible from the operator holding his pipette in a natural way.
  • the arrangement of counting disks and sprockets of training allows in particular, without increasing the body volume of the pipette, to arrange the eject button 10 above the butt of the outer sleeve, which is ergonomically favorable, the thumb of the user exercising more easily press the eject button when it comes on top of the slice of the hand that when it deviates from it, which would be the case if the button ejection had been placed on the opposite side.

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Abstract

A mechanical piston pipette comprises a pipette body, a piston/cylinder assembly and a tip ejector device. The pipette body accommodates an ejection actuation device and a piston actuation device effecting given longitudinal translational travel between a low limit stop position and a high limit stop position, with a travel modifying mechanism, pipetted volume adjustment mechanism and pipetted volume display mechanism. A mechanical piston pipette comprises a pipette body, a piston/cylinder assembly and a tip ejector device. The cylinder is arranged to be able to be equipped with a replaceable tip. The tip ejector device is movable along the cylinder to eject a tip from the end of the cylinder. The pipette body accommodates an ejection actuation device and a piston actuation device effecting given longitudinal translational travel between a low limit stop position and a high limit stop position in order to perform the aspiration of an adjustable volume of liquid, with travel modifying mechanism, pipetted volume adjustment mechanism, pipetted volume display mechanism, and calibration mechanism. The adjustment mechanism, display mechanism and calibration mechanism cooperate as an adjustment and calibration device in which, during a calibration operation, the display mechanism is regulated to make the displayed volume equate with the pipetted real volume. The adjustment and calibration device is housed inside the pipette body.

Description

La présente invention concerne une pipette à piston mécanique comprenant un corps de pipette, un ensemble piston-cylindre, le cylindre étant agencé de façon à pouvoir être muni d'un embout remplaçable, et un dispositif éjecteur d'embout, déplaçable le long du cylindre pour éjecter un embout de l'extrémité du cylindre, le dit corps de pipette accueillant un dispositif d'actionnement d'éjection et un dispositif d'actionnement de piston effectuant une course déterminée en translation longitudinale entre une position de butée basse et une position de butée haute pour effectuer l'aspiration et le dosage de liquides, avec des moyens de modification de la dite course.The present invention relates to a piston pipette mechanical device comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged so that it can be with replaceable tip and ejector endpiece, movable along the cylinder to eject a tip of the end of the cylinder, the said pipette body accommodating an ejection actuating device and a piston actuating device performing a stroke determined in longitudinal translation between a position of low stop and a high stop position to perform aspiration and dosing of liquids, with means of modification of the said race.

De très nombreux types de pipettes à piston sont connus dans l'état de la technique. On distingue les pipettes mécaniques dont le mécanisme est entièrement actionné par la main de l'utilisateur. Lorsque ces pipettes possèdent un moyen d'affichage du volume, celui-ci peut être analogique, digital et mécanique, ou encore électronique. Il existe également de nombreux types de pipettes à piston actionnés par un moteur électrique et dont les dispositifs d'affichage ou les dispositifs de réglage ou de calibrage sont également effectués par des moyens électro-mécaniques ou électroniques. La présente invention concerne exclusivement des pipettes à piston mécaniques, à volume fixe ou réglable. Lorsque le volume est réglable, l'invention concerne exclusivement les pipettes dont l'affichage de volume est lui-même mécanique.Many types of piston pipettes are known in the state of the art. We distinguish pipettes mechanical devices whose mechanism is entirely actuated by the hand of the user. When these pipettes have a way volume display, this one can be analog, digital and mechanical, or electronic. There are also many types of piston pipettes powered by an engine electrical equipment and whose display devices or adjustment or calibration devices are also performed by electromechanical or electronic means. The present invention relates exclusively to pipettes to mechanical piston, with fixed or adjustable volume. When the volume is adjustable, the invention relates exclusively to pipettes whose volume display itself is mechanical.

Pour des opérations hautement répétitives de laboratoires d'analyse, par exemple dans des kits d'analyse, on préfère utiliser des pipettes à volume fixe. Pour des opérations peu répétitives ou encore dans les laboratoires de recherche, on préfère généralement utiliser des pipettes à volume réglable, permettant une plus vaste gamme d'utilisation. Même si on ne prend en considération que la gamme des volumes 1µl à 1000 µl, qui concerne les pipettes appelées couramment micro-pipettes, alors que les pipettes dites "macro-pipettes" couvrent les gammes de volumes supérieures, une gamme complète de micro-pipettes représente en général plus de 20 objets différents présents au catalogue d'un fabricant. Un très grand souci des fabricants est donc la réduction du nombre total de pièces différentes entrant dans la constitution des différentes micro-pipettes d'une même série. Il est en particulier souhaitable de pouvoir utiliser le plus grand nombre possible de pièces communes pour réaliser des micro-pipettes à volume fixe et à volume variable, dans les gammes de volumes comparables.For highly repetitive laboratory operations for analysis, for example in test kits, it is preferred to use fixed volume pipettes. For small operations repetitive or in research laboratories, generally prefers to use volume-adjustable pipettes, allowing a wider range of use. Even if we do not takes into consideration that the range of volumes 1μl to 1000μl, which concerns the pipettes commonly called micro-pipettes, whereas pipettes called "macro-pipettes" cover the higher volume ranges, a full range of micro-pipettes usually represents more than 20 different objects present in a manufacturer's catalog. A great concern for manufacturers is therefore reducing the total number of parts different entering the constitution of the different micro-pipettes of the same series. He is particularly desirable to be able to use as many people as possible of common parts to make volume micro-pipettes fixed and variable volume, in the volume ranges comparable.

Dans certaines pipettes à piston de l'état de la technique, il est nécessaire de retirer plusieurs pièces du corps ou du dispositif d'affichage pour opérer un réglage ou une calibration, avec les risques d'égarer ces pièces qui en résultent.In certain piston pipettes of the state of the art, it is necessary to remove several parts of the body or display device for operating a setting or a calibration, with the risk of misplacing these parts result.

Un autre souci des fabricants est de réaliser des pipettes à piston ergonomiques, permettant de nombreuses manipulations répétitives sans fatigue excessive de la main, en particulier du pouce de l'utilisateur, et en particulier des pipettes à volume variable, dont le corps est suffisamment compact pour se loger confortablement dans le creux de la main de l'utilisateur, même si celui-ci a une main de petite taille.Another concern of manufacturers is to make pipettes ergonomic piston, allowing many manipulations repetitive without excessive hand fatigue, especially of the user's thumb, and in particular pipettes to variable volume, whose body is compact enough to to stay comfortably in the hollow of the hand of the user, even if he has a small hand.

Par souci ergonomique, il est également souhaitable de limiter le corps de pipette à la partie substantiellement cylindrique tenue dans la paume de la main et de supprimer dans la mesure du possible les parties supérieures proéminentes volumineuses formant une crosse en "L" ou une tête qui, dans les pipettes de l'état de la technique, sont souvent utilisées pour loger les dispositifs d'affichage et qui déplacent le centre de gravité de l'objet vers le haut.For ergonomic reasons, it is also desirable to limit the pipette body to the part substantially cylindrical holding in the palm of the hand and remove as far as possible the upper parts protruding bulges forming an "L" butt or which, in pipettes of the state of the art, are often used to house display devices and which move the center of gravity of the object upwards.

Enfin, il est souhaitable d'avoir un contrôle visuel du dispositif d'affichage et de calibration en cours de pipetage, c'est à dire pendant une phase de travail où, en général, la paume de la main est orientée approximativement en direction du visage de l'utilisateur, et d'agencer ce dispositif en conséquence.Finally, it is desirable to have a visual control of the display and calibration device during pipetting, that is to say during a work phase where, in general, the palm of the hand is oriented approximately in the direction of of the user's face, and to arrange this device in result.

Ces buts sont atteints dans une pipette à volume réglable, comprenant un corps de pipette, un ensemble piston-cylindre, le cylindre étant agencé de façon à pouvoir être muni d'un embout remplaçable, et un dispositif éjecteur d'embout, déplaçable le long du cylindre pour éjecter un embout de l'extrémité du cylindre, le dit corps de pipette accueillant un dispositif d'actionnement d'éjection et un dispositif d'actionnement de piston effectuant une course déterminée en translation longitudinale entre une position de butée basse et une position de butée haute pour effectuer l'aspiration de liquides, avec des moyens de modification de la dite course, des moyens de réglage du volume pipeté, des moyens d'affichage du volume pipeté et des moyens de calibration, par le fait que les dits moyens de réglage, les dits moyens d'affichage et les dits moyens de calibration coopèrent en un dispositif de réglage et de calibration dans lequel, lors d'une opération de calibration, les dits moyens d'affichage sont ajustés de façon à faire coïncider le volume affiché avec le volume réel pipeté et que le dit dispositif de réglage et de calibration est logé à l'intérieur du corps de pipette.These goals are achieved in a pipette with adjustable volume, comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged so as to be provided with a replaceable tip, and a tip ejector device, movable along the cylinder to eject a mouthpiece the end of the cylinder, the so-called pipette body welcoming an ejection actuating device and a device piston actuator performing a determined stroke in longitudinal translation between a low stop position and a high stop position to perform the suction of liquids, with means for modifying said stroke, means for adjusting the pipetted volume, display means pipetting volume and calibration means, by the fact that said adjustment means, said display means and the said calibration means cooperate in a device for setting and calibration in which, during an operation of calibration, the said display means are adjusted so to match the displayed volume with the actual pipetted volume and that said adjustment and calibration device is housed inside the pipette body.

Ces buts sont en particulier atteints dans une telle pipette à volume réglable ou dans une pipette à volume fixe du type défini d'entrée, par le fait qu'une pièce définissant la butée basse de la dite course passe d'une position totalement verrouillée à une position où elle peut effectuer un mouvement transversal, que, dans la position verrouillée, elle empêche le déplacement longitudinal d'une pièce définissant la butée haute de course et que dans la dite position déverrouillée, elle permet l'action de moyens de calibration déplaçant la position longitudinale de la dite butée haute de course.These goals are particularly achieved in such a pipette with adjustable volume or in a fixed volume pipette defined type of input, by the fact that a part defining the bottom stop of the said stroke passes from a position totally locked to a position where she can move transversal, that, in the locked position, it prevents the longitudinal displacement of a part defining the stop high of the race and that in the said unlocked position, it allows the action of calibration means displacing the longitudinal position of said high travel stop.

Des modes d'exécution préférés de la pipette à piston selon l'invention sont définis dans les revendications dépendantes. Preferred embodiments of the piston pipette according to the invention are defined in the dependent claims.

D'autres particularités et avantages de l'invention apparaítront à l'homme du métier de la description ci-dessous de deux modes d'exécution de corps de pipette, en référence au dessin, dans lequel

  • la figure 1 montre une coupe selon l'axe longitudinal d'un corps de pipette à volume réglable;
  • la figure 2 montre une coupe selon l'axe longitudinal d'un corps de pipette à volume fixe.
Other features and advantages of the invention will be apparent to those skilled in the art from the following description of two embodiments of pipette bodies, with reference to the drawing, in which
  • Figure 1 shows a section along the longitudinal axis of a pipette body with adjustable volume;
  • Figure 2 shows a section along the longitudinal axis of a fixed volume pipette body.

Les pièces communes aux corps de pipette des figs. 1 et 2 sont désignées par les mêmes numéros de référence.The parts common to the pipette bodies figs. 1 and 2 are designated by the same reference numbers.

Le corps de pipette à volume réglable montré par la figure 1 comprend un manchon extérieur 14 constitué lui-même d'une partie longitudinale 14a, sensiblement cylindrique mais avec un léger renflement ergonomique pour s'adapter à la paume de la main, à section transversale sensiblement elliptique, dont la partie supérieure se prolonge latéralement par une crosse 14b en forme de virgule destinée à reposer sur le flanc de l'index de la main de l'utilisateur. Le manchon extérieur 14 accueille un manchon intérieur 13 qui se loge dans le manchon extérieur 14 du même côté que la crosse 14b. Le manchon intérieur 13 présente une portion de paroi intérieure cylindrique avec une section en arc de cercle, qui forme avec la surface intérieure de la zone opposée du manchon extérieur 14 un logement cylindrique de section circulaire. Le manchon intérieur 13 est suffisamment épais pour accueillir le dispositif d'éjection d'embout, qui comprend un bouton éjecteur 10 surmonté d'un capuchon protecteur 9. Le bouton éjecteur se prolonge par une tige d'éjection 18 sur laquelle sont enfilés un ressort d'éjection 12 et une douille 11. Le manchon intérieur 13 loge également un tube à pignons 19 comprenant une pluralité de pignons rotatifs, par exemple trois pignons 19a, 19b, 19c, tournant autour d'un axe parallèle à l'axe longitudinal de la pipette et coopérant avec le dispositif de réglage et de calibration comme il sera décrit plus loin. Le haut du manchon 13 se prolonge latéralement par une bague 13a, coaxiale avec le logement cylindrique, qui coiffe l'ouverture supérieure du manchon 14.The adjustable volume pipette body shown in the figure 1 comprises an outer sleeve 14 which itself consists of a longitudinal portion 14a, substantially cylindrical but with a slight ergonomic bulge to fit the palm of the hand, with a substantially elliptical cross-section, of which the upper part is extended laterally by a stick 14b in a comma form intended to rest on the flank of the index of the user's hand. The outer sleeve 14 accommodates an inner sleeve 13 which fits into the sleeve outside 14 on the same side as the butt 14b. The sleeve interior 13 has an inner wall portion cylindrical with an arcuate section, which forms with the inner surface of the opposite zone of the outer sleeve 14 a cylindrical housing of circular section. The sleeve interior 13 is thick enough to accommodate the tip ejection device, which includes a button ejector 10 surmounted by a protective cap 9. The button ejector is extended by an ejector rod 18 on which are threaded an ejection spring 12 and a sleeve 11. The inner sleeve 13 also houses a pinion tube 19 comprising a plurality of rotary gears, for example three pinions 19a, 19b, 19c, rotating about an axis parallel to the longitudinal axis of the pipette and cooperating with the setting and calibration device as it will be described later. The top of the sleeve 13 is extended laterally by a ring 13a, coaxial with the housing cylindrical, which caps the upper opening of the sleeve 14.

Un bouton doseur 3 dépasse du sommet de la bague 13a. Le bouton doseur 3 est destiné à être enfoncé dans le corps de pipette par le pouce de l'utilisateur pendant les opérations de pipetage, à savoir aspiration de liquide dans l'embout jetable et éjection de ce liquide. Le bouton doseur 3 est repoussé vers le haut par le ressort de pipetage 7, de sorte à effectuer des déplacements alternatifs en translation selon l'axe longitudinal du corps de pipette. Le sommet du bouton doseur 3 est bouché par un capuchon 1. Le capuchon 1 est facilement enlevé par l'utilisateur au moyen d'un ongle. Il peut être réalisé dans différentes couleurs pour faciliter l'identification visuelle de la pipette.A metering knob 3 protrudes from the top of the ring 13a. The metering button 3 is intended to be embedded in the body of pipette by the user's thumb during operations pipetting, namely aspiration of liquid into the mouthpiece Disposable and ejection of this liquid. The doser button 3 is pushed upwards by the pipetting spring 7, so that perform alternative translational movements according to the longitudinal axis of the pipette body. The top of the button metering device 3 is plugged by a cap 1. The cap 1 is easily removed by the user by means of a fingernail. he can be realized in different colors to facilitate visual identification of the pipette.

Le bouton doseur 3 est fileté intérieurement. Il reçoit une vis de réglage 4 filetée extérieurement, de sorte que la rotation du bouton doseur 3 entraíne la vis de réglage 4 en translation longitudinale, lorsque celle-ci est bloquée elle-même en rotation comme décrit plus loin. Sur la figure 1, la vis de réglage 4 est représentée dans sa position extrême inférieure. La partie supérieure de la vis de réglage 4 est évidée et reçoit une clé de calibration 2 qui peut coulisser en translation longitudinale dans l'évidement de la vis de réglage 4, mais qui y est bloquée en rotation. Pour ce faire, l'évidement de la vis de réglage 4 et la clé de calibration peuvent être toutes deux de section carrée. La clé de calibration est enfilée dans un anneau 5.The metering knob 3 is threaded internally. He receives a adjusting screw 4 threaded externally, so that the rotation of the metering knob 3 causes the adjusting screw 4 longitudinal translation, when it is blocked itself in rotation as described below. In Figure 1, the adjusting screw 4 is shown in its extreme position lower. The upper part of the adjusting screw 4 is recessed and receives a calibration key 2 which can slide in longitudinal translation in the recess of the screw of setting 4, but which is locked in rotation. To do this, the recess of the adjusting screw 4 and the calibration key can both be square. The key to calibration is threaded into a ring 5.

La partie médiane de la vis de réglage 4 porte un renflement 4a, venu d'une pièce avec le corps de la vis 4, sur lequel repose un ressort de sur-course 4b. L'autre extrémité du ressort de sur-course 4b repose sur une butée de sur-course 4c emmanchée sur et solidarisée avec le corps de la vis de réglage 4. The middle part of the adjusting screw 4 carries a bulge 4a, come from one piece with the body of the screw 4, on which rests an over-stroke spring 4b. The other extremity of the overtravel spring 4b rests on an overtravel stop 4c fitted on and secured with the body of the screw setting 4.

Le dispositif de réglage et de calibration est désigné dans son ensemble par 8. Il comprend un disque de calibration 8a. Le disque de calibration 8a comprend un canon supérieur 8b entourant le corps de la vis de réglage 4, qui y coulisse longitudinalement, et un flasque inférieur 8c de diamètre supérieur à celui du canon 8b. Le disque de calibration 8a repose sur le fond 14c du manchon extérieur 14 et limite ainsi la course de la vis 4, lorsque la face inférieure de la butée 4c entre en contact avec lui. Un tube de réglage 8d repose sur le replat horizontal 8h circulaire formé entre le flasque 8c et le canon 8b. Le tube de réglage 8d et le disque de calibration 8a sont accouplés par un système de crabots de couplage C, constitué de deux couronnes dentées se faisant face, formées respectivement sur le replat 8h du disque de calibration et sur la face frontale inférieure du tube de réglage 8d. Les dents ont une hauteur faible et se désengagent facilement. Le manchon extérieur 14 loge, au niveau du disque de calibration 8a, un dispositif de verrouillage constitué d'un patin 15 muni d'une agrafe élastique 16. En position verrouillée, ce patin 15 empêche la rotation du disque de calibration 8a. Le patin est manoeuvrable de l'extérieur, par un trou du manchon 14, par n'importe quel objet ayant une pointe fine.The adjustment and calibration device is designated in its set by 8. It includes a calibration disk 8a. The calibration disk 8a comprises an upper barrel 8b surrounding the body of the adjusting screw 4, which slides therein longitudinally, and a lower flange 8c in diameter greater than that of the barrel 8b. The calibration disk 8a rests on the bottom 14c of the outer sleeve 14 and thus limits the stroke of the screw 4, when the lower face of the stop 4c gets in touch with him. An 8d adjustment tube is based on 8h circular horizontal plate formed between flange 8c and the barrel 8b. The adjustment tube 8d and the disc of calibration 8a are coupled by a dog clutch system coupling C, consisting of two toothed crowns being face, respectively formed on the 8h replat of the disk of calibration and on the lower end face of the tube 8d setting. Teeth are low in height and disengage easily. The outer sleeve 14 houses, at the disc level 8a, a locking device consisting of a shoe 15 provided with an elastic clip 16. In position locked, this pad 15 prevents the rotation of the disk from calibration 8a. The pad is manoeuvrable from the outside, by a hole of the sleeve 14, by any object having a fine point.

Le disque de calibration 8a est rendu solidaire en rotation du corps de la vis de réglage 4 au moyen de nervures/rainures longitudinales conjuguées. Le sommet du tube de réglage 8d entoure le bas du bouton doseur 3. Le tube de réglage 8d et le bouton doseur 3 sont également solidarisés en rotation par des nervures/rainures conjuguées.The calibration disk 8a is made integral in rotation the body of the adjusting screw 4 by means of ribs / grooves longitudinal conjugates. The top of the 8d adjustment tube surrounds the bottom of the metering knob 3. The 8d adjustment tube and the metering button 3 are also secured in rotation by ribs / conjugate grooves.

Le tube de réglage présente une partie inférieure de diamètre extérieur légèrement diminué, formant un ressaut circulaire horizontal. Le tube de réglage 8d porte d'autre part sur cette partie inférieure une pluralité de bagues de comptage, par exemple trois bagues 8e, 8f, 8g portant sur leur périphérie des chiffres de 0 à 9, fournissant une indication digitale du volume de liquide pipeté.The adjustment tube has a lower part of outer diameter slightly decreased, forming a protrusion horizontal circular. The 8d adjustment tube carries other share on this lower part a plurality of rings of counting, for example three rings 8e, 8f, 8g bearing on their periphery of the digits from 0 to 9, providing an indication digital volume of liquid pipetted.

Le manchon extérieur 14 comporte sur son côté opposé à la crosse 14b et à hauteur des bagues de comptage une fenêtre transparente 6 permettant de lire l'indication de volume.The outer sleeve 14 has on its side opposite the butt 14b and at the height of the counting rings a window transparent 6 to read the volume indication.

Le ressaut du tube de réglage 8d faisant face aux bagues de comptage comporte plusieurs dentures entraínant le pignon 19a. Les bagues de comptage comportent une denture continue sur la face frontale supérieure mais une seule indentation sur la face frontale opposée, de sorte que la rotation du disque de réglage 8d entraíne le premier pignon, qui à son tour entraíne le premier disque 8e qui, par exemple au bout d'un tour complet, entraíne le deuxième pignon 19b faisant tourner d'un digit la deuxième bague de comptage, et ainsi de suite. Ce mécanisme est en soi connu.The projection of the adjustment tube 8d facing the rings of counting comprises several teeth causing the pinion 19a. The counting rings have a continuous toothing on the upper frontal face but only one indentation on the opposite end face, so that the rotation of the disc of setting 8d drives the first pinion, which in turn drives the first 8th disc which, for example after a lap complete, drives the second pinion 19b rotating a digit the second counting ring, and so on. This mechanism is in itself known.

Le réglage du volume de liquide aspiré/dispensé par la pipette en mode normal de travail fonctionne comme suit:The adjustment of the volume of liquid sucked / dispensed by the pipette in normal working mode works as follows:

Le patin de verrouillage 15 est en position verrouillé, empêchant la rotation du disque de calibration 8a. Le disque de calibration bloque la rotation de la vis de réglage 4, qui elle-même est solidaire en rotation de la clé de calibrage 2. L'utilisateur fait tourner le bouton doseur 3, qui, par son filetage intérieur, entraíne en translation la vis de réglage 4. Ce déplacement en translation longitudinale opère le réglage de la course s'étendant entre le bas de la butée de course 4c et le haut du disque de calibrage 8a. Cette course détermine elle-même le volume de liquide, puisque la vis de réglage 4 est reliée au piston de la pipette par son extrémité inférieure 4d en forme de tête sphérique constituant une connexion détachable.The locking shoe 15 is in the locked position, preventing the rotation of the calibration disk 8a. The disc calibration locks the rotation of the adjusting screw 4, which it itself is integral in rotation with the calibration key 2. The user rotates the metering knob 3, which, by its internal thread, drives the adjusting screw 4. This displacement in longitudinal translation operates on the stroke adjustment extending between the bottom of the stop of race 4c and the top of the calibration disc 8a. This course determines itself the volume of liquid, since the screw of setting 4 is connected to the piston of the pipette by its end 4d in the form of a spherical head constituting a detachable connection.

Simultanément, la rotation du bouton doseur 3 entraíne en rotation le tube de réglage 8d et, via le tube de pignons 19, les disques compteurs 8e, 8f, 8g, comme exposé ci-dessus. A noter que le système de crabots C est désaccouplé, le tube 8d tourne sur le disque de calibration 8a, les dents des segments dentés ripant les unes au-dessus des autres.Simultaneously, the rotation of the metering knob 3 results in rotating the adjustment tube 8d and, via the gear tube 19, the 8th, 8f, 8g meter disks, as discussed above. AT note that the claw system C is uncoupled, the tube 8d turns on the calibration disk 8a, the teeth of the segments teeth ripping one above the other.

Après assemblage d'une pipette, la course, définie par la distance entre la butée de surcourse 4c et le disque de calibrage 8a ne correspond pas, dans la majorité des cas, au volume affiché. Une calibration est nécessaire à l'usine. L'utilisateur ultérieur peut également souhaiter périodiquement, par exemple pour des liquides spécifiques ou encore après des opérations de démontage, de nettoyage et de stérilisation, procéder à un recalibrage. Une opération de calibrage s'effectue de la manière suivante:After assembling a pipette, the stroke, defined by the distance between the overtravel stop 4c and the disc of 8a does not, in the majority of cases, correspond to displayed volume. A calibration is necessary at the factory. The subsequent user may also wish periodically, for example for specific liquids or again after dismantling, cleaning and sterilization, recalibrate. An operation of calibration is carried out as follows:

On mesure, par exemple en le pesant, le volume dispensé par la pipette. A titre d'exemple le volume mesuré peut être de 545µl alors que le compteur affiche 210µl. L'opération de calibrage consiste à amener la valeur de l'affichage à 545µl correspondant au volume effectif, sans modifier la course.We measure, for example by weighing it, the volume dispensed by the pipette. For example, the measured volume can be 545μl while the meter displays 210μl. The operation of calibration is to bring the value of the display to 545μl corresponding to the actual volume, without changing the stroke.

Pour ce faire, le patin de verrouillage 15 est déverrouillé, libérant ainsi la rotation du disque de calibration 8a. Le capuchon 1 est enlevé pour accéder à la vis de calibration 2. Celle-ci peut être actionnée soit par une pièce de monnaie ou un tournevis, soit, après avoir partiellement coulissé vers l'extérieur, peut être actionnée à la main. La rotation de la clé de calibration entraíne en rotation la vis de réglage 4, qui elle-même entraíne en rotation le disque de calibration. Pendant qu'il tourne la clé de calibration, l'utilisateur veille à ne pas toucher le bouton doseur 3 afin de ne pas modifier la position relative bouton 3/vis 4. Le disque de calibration entraíne le tube de réglage 8d en rotation, par l'intermédiaire du système de crabots C. La rotation du tube de réglage entraíne la rotation des pignons du tube de pignons 19 et par conséquent la rotation des bagues de comptage. La clé est tournée jusqu'à ce que la valeur mesurée, dans cet exemple 545µl, s'affiche. Ensuite, le patin 15 est repoussé en position de verrouillage afin de bloquer en position angulaire le disque de calibration 8a et la vis de réglage 4. La clé de calibration 2 est repoussée dans son logement et le capuchon est replacé. La valeur du dispositif compteur étant calquée sur la course, les réglages de volume ultérieurs de la pipette s'effectuent simplement par rotation du bouton doseur 3, qui modifie cette fois la course par déplacement en translation de la vis de réglage 4 sans déplacement en rotation de celle-ci.To do this, the locking shoe 15 is unlocked, thus freeing the rotation of the disc from calibration 8a. The cap 1 is removed to access the screw 2. This can be operated either by a coin or a screwdriver, either, after having partially slid outward, can be operated at the hand. The rotation of the calibration key leads to turning the adjusting screw 4, which itself leads to rotation the calibration disc. While turning the key the user is careful not to touch the doser button 3 so as not to change the relative position button 3 / screw 4. The calibration disc drives the tube of 8d setting in rotation, through the system of jaws C. The rotation of the adjustment tube causes the rotation pinions of the pinion tube 19 and therefore the rotation of the counting rings. The key is turned until the measured value, in this example 545μl, is displayed. Then, the pad 15 is pushed back into the locking position to lock in angular position the calibration disc 8a and the adjusting screw 4. The calibration key 2 is pushed back into its housing and the hood is replaced. The value of the counter device being modeled on the stroke, the subsequent volume adjustments of the pipette are made simply by rotating the metering knob 3, which modifies this times the stroke by displacement in translation of the screw of adjustment 4 without moving in rotation thereof.

Le corps de pipette à volume fixe montré par la figure 2 comprend un manchon extérieur 14 constitué lui-même d'une partie longitudinale 14a, sensiblement cylindrique mais avec un léger renflement ergonomique pour s'adapter à la paume de la main, à section transversale sensiblement elliptique, dont la partie supérieure se prolonge latéralement par une crosse 14b en forme de virgule destinée à reposer sur le flanc de l'index de la main de l'utilisateur. Le manchon extérieur 14 accueille un manchon intérieur 13 qui se loge dans le manchon extérieur 14 du même côté que la crosse 14b. Le manchon intérieur 13 présente une portion de paroi intérieure cylindrique avec une section en arc de cercle, qui forme avec la surface intérieure de la zone opposée du manchon extérieur 14 un logement cylindrique de section circulaire. Le manchon intérieur 13 est suffisamment épais pour accueillir le dispositif d'éjection d'embout, qui comprend un bouton éjecteur 10 surmonté d'un capuchon protecteur 9. Le bouton éjecteur se prolonge par une tige d'éjection 18 sur laquelle sont enfilés un ressort d'éjection 12 et une douille 11. L'emplacement du tube à pignons 19 décrit plus haut reste vide. Le haut du manchon 13 se prolonge latéralement par une bague 13a, coaxiale avec le logement cylindrique, qui coiffe l'ouverture supérieure du manchon 14.The fixed volume pipette body shown in Figure 2 comprises an outer sleeve 14 which itself consists of a longitudinal portion 14a, substantially cylindrical but with a slight ergonomic bulge to fit the palm of the hand, with a substantially elliptical cross-section, of which the upper part is extended laterally by a stick 14b in a comma form intended to rest on the flank of the index of the user's hand. The outer sleeve 14 accommodates an inner sleeve 13 which fits into the sleeve outside 14 on the same side as the butt 14b. The sleeve interior 13 has an inner wall portion cylindrical with an arcuate section, which forms with the inner surface of the opposite zone of the outer sleeve 14 a cylindrical housing of circular section. The sleeve interior 13 is thick enough to accommodate the tip ejection device, which includes a button ejector 10 surmounted by a protective cap 9. The button ejector is extended by an ejector rod 18 on which are threaded an ejection spring 12 and a sleeve 11. The location of the pinion tube 19 described above remains empty. The top of the sleeve 13 extends laterally by a ring 13a, coaxial with the cylindrical housing, which cap the upper opening of the sleeve 14.

Un bouton doseur 3 dépasse du sommet de la bague 13a. Le bouton doseur 3 est destiné à être enfoncé dans le corps de pipette par le pouce de l'utilisateur pendant les opérations de pipetage, à savoir aspiration de liquide dans l'embout jetable et éjection de ce liquide. Le bouton doseur 3 est repoussé vers le haut par le ressort de pipetage 7, de sorte à effectuer des déplacements alternatifs en translation selon l'axe longitudinal du corps de pipette. Le sommet du bouton doseur 3 est bouché par un capuchon 1. Le capuchon 1 est facilement enlevé par l'utilisateur au moyen d'un ongle. Il peut être réalisé dans différentes couleurs pour faciliter l'identification visuelle de la pipette.A metering knob 3 protrudes from the top of the ring 13a. The metering button 3 is intended to be embedded in the body of pipette by the user's thumb during operations pipetting, namely aspiration of liquid into the mouthpiece Disposable and ejection of this liquid. The doser button 3 is pushed upwards by the pipetting spring 7, so that perform alternative translational movements according to the longitudinal axis of the pipette body. The top of the button metering device 3 is plugged by a cap 1. The cap 1 is easily removed by the user by means of a fingernail. he can be realized in different colors to facilitate visual identification of the pipette.

Le bouton doseur 3 est fileté intérieurement. Il reçoit une vis de réglage 4 filetée extérieurement. Sur la figure 2, la vis de réglage 4 est représentée dans sa position extrême inférieure. La partie supérieure de la vis de réglage 4 est évidée et reçoit une clé de calibration 2 qui peut coulisser en translation longitudinale dans l'évidement de la vis de réglage 4, mais qui y est bloquée en rotation. Pour ce faire, l'évidement de la vis de réglage 4 et la clé de calibration peuvent être toutes deux de section carrée. La clé de calibration est enfilée dans un anneau 5.The metering knob 3 is threaded internally. He receives a adjusting screw 4 externally threaded. In Figure 2, the adjusting screw 4 is shown in its extreme position lower. The upper part of the adjusting screw 4 is recessed and receives a calibration key 2 which can slide in longitudinal translation in the recess of the screw of setting 4, but which is locked in rotation. To do this, the recess of the adjusting screw 4 and the calibration key can both be square. The key to calibration is threaded into a ring 5.

La partie médiane de la vis de réglage 4 porte un renflement 4a, venu d'une pièce avec le corps de la vis 4, sur lequel repose un ressort de sur-course 4b. L'autre extrémité du ressort de sur-course 4b repose sur une butée de sur-course 4c emmanchée sur et solidarisée avec le corps de la vis de réglage 4.The middle part of the adjusting screw 4 carries a bulge 4a, come from one piece with the body of the screw 4, on which rests an over-stroke spring 4b. The other extremity of the overtravel spring 4b rests on an overtravel stop 4c fitted on and secured with the body of the screw setting 4.

Le dispositif de calibration est désigné dans son ensemble par 8'. Il comprend un disque de calibration 8a. Le disque de calibration 8a comprend un canon supérieur 8b entourant le corps de la vis de réglage 4, qui y coulisse longitudinalement, et un flasque inférieur 8c de diamètre supérieur à celui du canon 8b. Le disque de calibration 8a repose sur le fond 14c du manchon extérieur 14 et limite ainsi la course de la vis 4, lorsque la face inférieure de la butée 4c entre en contact avec lui. Le disque de calibration 8a peut être identique à celui montré sur la figure 1, et porter une couronne dentée circulaire, formée sur le replat 8h du disque de calibration; mais, dans le cas d'une pipette à volume fixe, cette couronne n'a pas de fonction. Le manchon extérieur 14 loge, au niveau du disque de calibration 8a, un dispositif de verrouillage constitué d'un patin 15 muni d'une agrafe élastique 16. En position verrouillée, ce patin 15 empêche la rotation du disque de calibration 8a. Le patin est manoeuvrable de l'extérieur, par un trou du manchon 14, par n'importe quel objet ayant une pointe fine. Le disque de calibration 8a est rendu solidaire en rotation du corps de la vis de réglage 4 au moyen de nervures/rainures longitudinales conjuguées.The calibration device is designated as a whole by 8 '. It includes a calibration disk 8a. The record of calibration 8a comprises an upper barrel 8b surrounding the body of the adjusting screw 4, which slides there longitudinally, and a lower flange 8c in diameter greater than that of the barrel 8b. The calibration disk 8a rests on the bottom 14c of the outer sleeve 14 and thus limits the stroke of the screw 4, when the lower face of the stop 4c gets in touch with him. The calibration disk 8a can be identical to that shown in Figure 1, and bear a Circular toothed crown, formed on the plate 8h of the disc calibration; but, in the case of a fixed volume pipette, this crown has no function. The outer sleeve 14 at the level of the calibration disk 8a, a device for lock consisting of a shoe 15 provided with a clip 16. In the locked position, this pad 15 prevents the rotation of the calibration disk 8a. The pad is manoeuvrable from the outside, through a hole in the sleeve 14, by any object having a fine point. The calibration disk 8a is secured in rotation of the body of the adjusting screw 4 to means of ribs / longitudinal grooves conjugate.

Un tube 8d' repose sur le replat horizontal circulaire formé entre le flasque 8c et le canon 8b. Mais la face frontale inférieure du tube de réglage 8d' est de préférence lisse. Le sommet du tube 8d' entoure le bas du bouton doseur 3. Le tube 8d' et le bouton doseur 3 sont également solidarisés en rotation par des nervures/rainures conjuguées. D'autre part, le tube 8d' porte deux nervures extérieures longitudinales qui pénètrent dans des rainures longitudinales du manchon intérieur 13. Le tube 8d' et le bouton doseur 3 sont donc tous deux bloqués en rotation.A tube 8d 'rests on the circular horizontal plate formed between the flange 8c and the barrel 8b. But the face bottom end of the adjustment tube 8d 'is preferably smooth. The top of the tube 8d 'surrounds the bottom of the doser button 3. The tube 8d 'and the metering knob 3 are also secured in rotation by conjugated ribs / grooves. On the other hand, the tube 8d 'carries two outer ribs longitudinal penetrating into longitudinal grooves of the inner sleeve 13. The tube 8d 'and the metering knob 3 are both locked in rotation.

Le tube 8d' présente comme le tube 8d décrit plus haut une partie inférieure de diamètre extérieur légèrement diminué, formant un ressaut circulaire horizontal. Mais le tube 8d' ne porte pas de bagues de comptage.The tube 8d 'has like the tube 8d described above a lower part of outer diameter slightly decreased, forming a horizontal circular projection. But the tube 8d 'does wears no counting rings.

Le manchon extérieur 14 comporte sur son côté opposé à la crosse 14b et à hauteur de la partie inférieure du tube 8d une plaquette opaque 6' remplaçant la fenêtre 6.The outer sleeve 14 has on its side opposite the 14b stock and at the height of the lower part of the tube 8d a opaque plate 6 'replacing window 6.

Après assemblage d'une pipette, la course, définie par la distance entre la butée de surcourse 4c et le disque de calibrage 8a ne correspond pas, dans la majorité des cas, au volume nominal. Une calibration est nécessaire à l'usine. L'utilisateur ultérieur peut également souhaiter périodiquement, par exemple après des opérations de démontage, de nettoyage et de stérilisation, procéder à un recalibrage. Une opération de calibrage s'effectue de la manière suivante: After assembling a pipette, the stroke, defined by the distance between the overtravel stop 4c and the disc of 8a does not, in the majority of cases, correspond to nominal volume. A calibration is necessary at the factory. The subsequent user may also wish periodically, for example after disassembly operations, cleaning and sterilization, recalibrate. A calibration operation is performed as follows:

On mesure, par exemple en le pesant, le volume dispensé par la pipette. A titre d'exemple le volume mesuré peut être de 545µl alors que la valeur nominale est de 500µl. L'opération de calibrage consiste à amener la valeur du volume effectif à la valeur nominale.We measure, for example by weighing it, the volume dispensed by the pipette. For example, the measured volume can be 545μl while the nominal value is 500μl. The operation calibration is to bring the value of the actual volume to the nominal value.

Pour ce faire, le patin de verrouillage 15 est déverrouillé, libérant ainsi la rotation du disque de calibration 8a. Le capuchon 1 est enlevé pour accéder à la vis de calibration 2. Celle-ci peut être actionnée soit par un tournevis ou une pièce de monnaie, soit, après avoir partiellement coulissé vers l'extérieur, peut être actionnée à la main. La rotation de la clé de calibration entraíne en rotation la vis de réglage 4, qui elle-même entraíne en rotation le disque de calibration. Le disque de calibration glisse sous le tube 8d'. Simultanément, la vis de réglage se déplace en translation longitudinale, modifiant la course, et donc le volume pipeté. Après mesure de ce volume, l'opération peut être répétée jusqu'à ce que la valeur mesurée coïncide avec la valeur nominale ou avec une autre valeur désirée par l'utilisateur. Ensuite, le patin 15 est repoussé en position de verrouillage afin de bloquer en position angulaire le disque de calibration 8a et la vis de réglage 4. La clé de calibration 2 est repoussée dans son logement et le capuchon est replacé.To do this, the locking shoe 15 is unlocked, thus freeing the rotation of the disc from calibration 8a. The cap 1 is removed to access the screw 2. This can be operated either by a screwdriver or a coin, that is, after having partially slid outward, can be operated at the hand. The rotation of the calibration key leads to turning the adjusting screw 4, which itself leads to rotation the calibration disc. The calibration disc slides under the tube 8d '. At the same time, the adjusting screw moves in longitudinal translation, modifying the stroke, and so the volume pipetted. After measuring this volume, the operation can be repeated until the measured value coincides with the nominal value or with another desired value by the user. Then, the pad 15 is pushed back into position lock in order to lock in the angular position the calibration disk 8a and the adjusting screw 4. The key of calibration 2 is pushed back into its housing and the cap is replaced.

Il résulte de la description ci-dessus que le corps de pipette à volume variable et le corps de pipette à volume fixe utilisent, dans une même gamme de volume, une grande majorité de pièces communes. Celles-ci sont identifiées par des numéros de référence identiques dans les figures 1 et 2. L'homme du métier observera en particulier qu'une même pièce, la bague de calibration 8a, assure plusieurs fonctions, à savoir de butée de fin de course, de réglage et/ou de calibration dans les deux types de pipette. Une seule pièce essentielle, à savoir le tube 8d, respectivement 8d', a des structures et des fonctions différentes: le tube 8d sert à la fois de guide au bouton doseur 3, de logement au ressort 7 et assure une fonction de réglage comme exposé ci-dessus. Le tube 8d' qui diffère essentiellement du tube 8d par la présence de nervures extérieures le bloquant en rotation, ne sert que de guide et de logement. On notera que les tubes 8d et 8d' ont par ailleurs des dimensions identiques. Le corps de pipette à volume réglable dispose par ailleurs d'un ensemble de bagues de comptage et de pignons d'entraínement décrits ci-dessus, qui ne sont pas présents dans le corps de la pipette à volume fixe.It follows from the description above that the body of variable volume pipette and fixed volume pipette body use, in the same volume range, a large majority common rooms. These are identified by numbers identical reference numbers in figures 1 and 2. The man of the profession will observe in particular that the same piece, the ring of calibration 8a, provides several functions, namely stop end of stroke, adjustment and / or calibration in the two types of pipette. One essential piece, namely the tube 8d, respectively 8d ', has structures and different functions: the tube 8d serves both as a guide for metering button 3, housing spring 7 and provides a adjustment function as explained above. The tube 8d which differs essentially from the tube 8d by the presence of ribs the outer blocking it in rotation, serves only as a guide and of housing. It will be noted that the tubes 8d and 8d 'have moreover identical dimensions. The pipette body to adjustable volume also has a set of rings counting and drive gears described above, that are not present in the body of the volume pipette fixed.

L'homme du métier observera en particulier que les dimensions du corps de la pipette à volume variable ne sont pas différentes des dimensions du corps de pipette à volume fixe, l'ensemble des bagues de comptage et des pignons d'entraínement ayant été logés respectivement dans l'encombrement global du tube de réglage 8d et coaxialement avec le dispositif d'éjection, à l'intérieur du manchon intérieur 13.The person skilled in the art will observe in particular that the Body dimensions of the variable volume pipette are only no different from the volume pipette body dimensions fixed, all counting rings and pinions have been housed respectively in the overall size of the adjustment tube 8d and coaxially with the ejection device, inside the sleeve inside 13.

L'ensemble du dispositif de réglage et de calibration est logé dans le corps de pipette longitudinal, sans augmentation de volume de celui-ci, et l'affichage, qui n'est pas masqué par le pouce, reste visible de l'opérateur tenant sa pipette d'une manière naturelle.The entire adjustment and calibration device is housed in the longitudinal pipette body, without increase volume of it, and the display, which is not hidden by the thumb, remains visible from the operator holding his pipette in a natural way.

L'agencement des disques de comptage et des pignons d'entraínement permet en particulier, sans augmentation du volume du corps de la pipette, d'agencer le bouton d'éjection 10 au-dessus de la crosse du manchon extérieur, ce qui est érgonomiquement favorable, le pouce de l'utilisateur exerçant plus facilement une pression sur le bouton d'éjection lorsqu'il vient au-dessus de la tranche de la main que lorsqu'il s'en écarte, ce qui serait le cas si le bouton d'éjection avait été placé sur le côté opposé.The arrangement of counting disks and sprockets of training allows in particular, without increasing the body volume of the pipette, to arrange the eject button 10 above the butt of the outer sleeve, which is ergonomically favorable, the thumb of the user exercising more easily press the eject button when it comes on top of the slice of the hand that when it deviates from it, which would be the case if the button ejection had been placed on the opposite side.

Claims (13)

Pipette à piston mécanique, à volume réglable, comprenant un corps de pipette, un ensemble piston-cylindre, le cylindre étant agencé de façon à pouvoir être muni d'un embout remplaçable, et un dispositif éjecteur d'embout, déplaçable le long du cylindre pour éjecter un embout de l'extrémité du cylindre, le dit corps de pipette accueillant un dispositif d'actionnement d'éjection et un dispositif d'actionnement de piston (3, 4, 7) effectuant une course déterminée en translation longitudinale entre une position de butée basse et une position de butée haute pour effectuer l'aspiration de liquides, avec des moyens de modification de la dite course, des moyens de réglage (4) du volume pipeté, des moyens d'affichage (8e, 8f, 8g) du volume pipeté et des moyens de calibration, caractérisée en ce que les dits moyens de réglage, les dits moyens d'affichage et les dits moyens de calibration coopèrent en un dispositif de réglage et de calibration (8) dans lequel, lors d'une opération de calibration, les dits moyens d'affichage sont ajustés de façon à faire coïncider le volume affiché avec le volume réel pipeté et en ce que le dit dispositif de réglage et de calibration (8) est logé à l'intérieur du corps de pipette.A mechanical piston pipette, with adjustable volume, comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged to be provided with a replaceable tip, and a tip ejector device movable along the cylinder for ejecting a tip from the end of the cylinder, said pipette body accommodating an ejection actuating device and a piston actuating device (3, 4, 7) performing a determined longitudinal translation stroke between a position of low stop and a high stop position for effecting the suction of liquids, with means for modifying said stroke, means (4) for adjusting the pipetted volume, display means (8e, 8f, 8g) pipette volume and calibration means, characterized in that said adjustment means, said display means and said calibration means cooperate in a setting and calibration device (8) in which, when a calibration operation, said display means are adjusted so as to make the displayed volume coincide with the actual volume pipetted and in that said adjustment and calibration device (8) is housed inside the body pipette. Pipette à piston mécanique, à volume réglable, selon la revendication 1, ou pipette à piston mécanique à volume fixe comprenant un corps de pipette, un ensemble piston-cylindre, le cylindre étant agencé de façon à pouvoir être muni d'un embout remplaçable, et un dispositif éjecteur d'embout, déplaçable le long du cylindre pour éjecter un embout de l'extrémité du cylindre, le dit corps de pipette accueillant un dispositif d'actionnement d'éjection et un dispositif d'actionnement de piston (3, 4, 7) effectuant une course déterminée en translation longitudinale entre une position de butée basse et une position de butée haute pour effectuer l'aspiration de liquides, avec des moyens de modification de la dite course, caractérisée en ce qu'une pièce (8a) définissant la butée basse de la dite course passe d'une position totalement verrouillée à une position où elle peut effectuer un mouvement transversal, en ce que, dans la position verrouillée, elle empêche le déplacement longitudinal d'une pièce (4c) définissant la butée haute de course et que dans la dite position déverrouillée, elle permet l'action de moyens (2, 3, 4) de calibration déplaçant la position longitudinale de la dite butée haute de course.An adjustable volume mechanical piston pipette according to claim 1, or a fixed volume mechanical piston pipette comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged to be provided with a replaceable tip, and a tip ejector device, movable along the cylinder for ejecting a tip from the end of the cylinder, said pipette body accommodating an ejection actuator and a piston actuator (3, 4). , 7) performing a determined longitudinal translation run between a low stop position and a high stop position to effect the aspiration of liquids, with means for modifying said stroke, characterized in that a piece (8a) defining the lower stop of said stroke passes from a fully locked position to a position where it can effect a transverse movement, in that , in the locked position, it prevents e the longitudinal displacement of a part (4c) defining the upper travel stop and that in the said unlocked position, it allows the action of calibration means (2, 3, 4) displacing the longitudinal position of said high abutment race. Pipette selon la revendication 2 caractérisée en ce que la dite pièce définissant la position de butée basse est un disque de calibration (8a) dont la position longitudinale est déterminée par des éléments fixes du corps de pipette et qui peut être verrouillée en rotation ou déverrouillée en rotation par des moyens de verrouillage (15, 16) accessibles de l'extérieur sans démontage d'autres éléments de la pipette.Pipette according to claim 2 characterized in that said piece defining the low stop position is a calibration disc (8a) whose longitudinal position is determined by fixed elements of the pipette body and which can be locked in rotation or unlocked in rotation by locking means (15, 16) accessible from the outside without disassembly of other elements of the pipette. Pipette selon la revendication 3 caractérisée en ce que le dit disque de calibration (8a) est coaxial d'une vis de réglage (4) dont il est solidaire en rotation, que la dite vis de réglage 4 est libre de coulisser longitudinalement par rapport au dit disque de calibration, l'extrémité inférieure (4d) de la dite vis de réglage actionnant le piston de la pipette, en ce que la dite vis de réglage porte une butée de surcourse (4c) solidaire de la dite vis de réglage (4), la distance entre la dite butée de surcourse (4c) et le dit disque de calibration (8a), la pipette étant au repos, définissant la dite course, et que la vis de réglage (4) est agencée de telle façon dans le corps de pipette qu'une rotation de la dite vis de réglage (4) s'accompagne nécessairement d'un mouvement hélicoïdal.Pipette according to claim 3 characterized in that said calibration disk (8a) is coaxial with a set screw (4) which it is integral in rotation, that said adjustment screw 4 is free to slide longitudinally relative to the said calibration disk, the lower end (4d) of said adjustment screw actuating the piston of the pipette, in that said adjustment screw carries an overtravel stop (4c) integral with said adjusting screw (4 ), the distance between said overtravel stop (4c) and said calibration disk (8a), the pipette being at rest, defining said stroke, and that the adjusting screw (4) is arranged in such a manner in the pipette body that a rotation of said adjustment screw (4) is necessarily accompanied by a helical movement. Pipette selon la revendication 4, caractérisée en ce que le dit déplacement hélicoïdal est obtenu au moyen de filetages longitudinaux reliant la dite vis de réglage à un bouton doseur (3) et en ce que le dit bouton doseur (3) constitue également l'organe permettant d'actionner la vis de réglage (4) pour effectuer les opérations de pipetage.Pipette according to claim 4, characterized in that said helical displacement is obtained by means of longitudinal threads connecting said adjusting screw to a metering knob (3) and in that said metering knob (3) also constitutes the organ allowing the adjusting screw (4) to be actuated to perform the pipetting operations. Pipette selon la revendication 5, caractérisée en ce que le dit bouton doseur (3) accueille une clé de calibration (2) solidaire en rotation de la dite vis de réglage (4) et libre de coulisser longitudinalement.Pipette according to claim 5, characterized in that said dosing button (3) accommodates a calibration key (2) integral in rotation with said adjustment screw (4) and free to slide longitudinally. Pipette selon la revendication 6, caractérisée en ce que pendant les opérations de pipetage, le bouton doseur (3) coulisse longitudinalement dans un tube (8d, 8d') dont il est solidaire en rotation.Pipette according to claim 6, characterized in that during the pipetting operations, the dosing knob (3) slides longitudinally in a tube (8d, 8d ') which it is integral in rotation. Pipette selon la revendication 7, caractérisée en ce que l'extrémité inférieure du dit tube (8d, 8d') repose sur le dit tube de calibration (8a).Pipette according to claim 7, characterized in that the lower end of said tube (8d, 8d ') rests on said calibration tube (8a). Pipette à volume fixe selon la revendication 8, caractérisée en ce que le dit tube (8d') est verrouillé en rotation par rapport au corps de la pipette.Fixed volume pipette according to claim 8, characterized in that said tube (8d ') is locked in rotation relative to the body of the pipette. Pipette à volume réglable selon la revendication 8, caractérisée en ce que le dit tube (8d) interagit avec le dit disque de calibration (8a) au moyen d'un dispositif à crabots (C) capable d'entraíner le dit tube (8d) en rotation.Adjustable volume pipette according to claim 8, characterized in that said tube (8d) interacts with said calibration disk (8a) by means of a jaw device (C) capable of driving said tube (8d) in rotation. Pipette à volume réglable selon la revendication 10, caractérisée par un dispositif d'affichage mécanique du volume (8e, 8f, 8g) asservi (19) à la rotation du dit tube (8d).Adjustable volume pipette according to claim 10, characterized by a mechanical volume display device (8e, 8f, 8g) slaved (19) to the rotation of said tube (8d). Pipette à volume réglable selon la revendication 11, caractérisée en ce que le dispositif d'affichage du volume comporte des bagues de comptage (8e, 8f, 8g) coaxiales avec le dit tube 8d et des pignons d'entraínement (19) logés avec le dispositif d'éjection. Adjustable volume pipette according to claim 11, characterized in that the volume display device comprises counting rings (8e, 8f, 8g) coaxial with said tube 8d and drive gears (19) housed with the ejection device. Pipette à volume réglable selon l'une des revendications 11 ou 12, caractérisée en ce que le dit dispositif d'affichage est logé dans la moitié inférieure du corps de pipette.Adjustable volume pipette according to one of claims 11 or 12, characterized in that said display device is housed in the lower half of the pipette body.
EP03405604A 2003-08-19 2003-08-19 Mechanical piston pipette Expired - Lifetime EP1514600B2 (en)

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AT03405604T ATE365075T1 (en) 2003-08-19 2003-08-19 MECHANICAL PISTON PIPETTE
EP03405604A EP1514600B2 (en) 2003-08-19 2003-08-19 Mechanical piston pipette
DE60314516T DE60314516T3 (en) 2003-08-19 2003-08-19 Mechanical piston pipette
ES03405604T ES2289252T5 (en) 2003-08-19 2003-08-19 Mechanical piston pipette
US10/916,359 US7204163B2 (en) 2003-08-19 2004-08-11 Mechanical piston pipette

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EP03405604A EP1514600B2 (en) 2003-08-19 2003-08-19 Mechanical piston pipette

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EP1514600A1 true EP1514600A1 (en) 2005-03-16
EP1514600B1 EP1514600B1 (en) 2007-06-20
EP1514600B2 EP1514600B2 (en) 2012-02-22

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US (1) US7204163B2 (en)
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AT (1) ATE365075T1 (en)
DE (1) DE60314516T3 (en)
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US5413006A (en) * 1992-09-28 1995-05-09 Gilson Medical Electronics (France) S.A. Pipette for sampling and dispensing adjustable volumes of liquids
EP0649678A1 (en) 1993-10-21 1995-04-26 Eppendorf-Netheler-Hinz Gmbh Plunger pump pipette
US5531131A (en) * 1993-10-21 1996-07-02 Eppendorf-Netheler-Hinz Gmbh Device for adjusting correction factor of a plunger lift pipet
US5650124A (en) * 1995-07-24 1997-07-22 Gilson; Warren E. Adjustable pipette
US5792424A (en) * 1996-03-05 1998-08-11 Rainin Instrument Co., Inc. Manual pipette with delayed-action home position latch
US6295880B1 (en) * 1999-12-08 2001-10-02 Warren E. Gilson Adjustable pipette
FR2807343A1 (en) * 2000-04-07 2001-10-12 Gilson Sa COLLECTION PIPETTE PROVIDED WITH MEANS FOR ADJUSTING THE VOLUME TO BE COLLECTED
EP1197264A2 (en) * 2000-10-11 2002-04-17 Thermo Labsystems Oy Pipette with adjustable volume

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2165765A1 (en) * 2008-09-12 2010-03-24 Eppendorf Ag Pipette device with double thread
FR3080050A1 (en) * 2018-04-17 2019-10-18 Gilson Sas DEVICE FOR LOCKING A VOLUME ADJUSTMENT SCREW FOR A PIPETTING SYSTEM
WO2019202246A1 (en) * 2018-04-17 2019-10-24 Gilson Sas Device for locking a volume adjustment screw for a pipetting system
CN111989160A (en) * 2018-04-17 2020-11-24 吉尔松有限合伙公司 Device for locking a capacity adjusting screw of a pipetting system
CN111989160B (en) * 2018-04-17 2022-02-11 吉尔松有限合伙公司 Device for locking a capacity adjusting screw of a pipetting system
US11559800B2 (en) 2018-04-17 2023-01-24 Gilson Sas Device for locking a volume adjustment screw for a pipetting system
DE102020002780B3 (en) 2020-05-11 2021-10-14 Brand Gmbh + Co Kg Pipette and method of operating a pipette
DE102021112246A1 (en) 2020-05-11 2021-11-11 Brand Gmbh + Co Kg Pipette and method of operating a pipette

Also Published As

Publication number Publication date
DE60314516D1 (en) 2007-08-02
ES2289252T3 (en) 2008-02-01
US20050039550A1 (en) 2005-02-24
EP1514600B2 (en) 2012-02-22
ES2289252T5 (en) 2012-06-12
DE60314516T2 (en) 2008-02-28
ATE365075T1 (en) 2007-07-15
EP1514600B1 (en) 2007-06-20
US7204163B2 (en) 2007-04-17
DE60314516T3 (en) 2012-07-19

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