EP0877652A1 - Sample vessel for taking blood samples - Google Patents

Sample vessel for taking blood samples

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Publication number
EP0877652A1
EP0877652A1 EP97949956A EP97949956A EP0877652A1 EP 0877652 A1 EP0877652 A1 EP 0877652A1 EP 97949956 A EP97949956 A EP 97949956A EP 97949956 A EP97949956 A EP 97949956A EP 0877652 A1 EP0877652 A1 EP 0877652A1
Authority
EP
European Patent Office
Prior art keywords
vessel
blood
sample
capillary
pct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP97949956A
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German (de)
French (fr)
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EP0877652B1 (en
Inventor
Walter Sarstedt
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Sarstedt AG and Co KG
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Sarstedt AG and Co KG
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Publication of EP0877652A1 publication Critical patent/EP0877652A1/en
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes

Definitions

  • the invention relates to a sample vessel for taking very small amounts of blood, e.g. from a patient's fingertip or earlobe, so that venous blood withdrawal is often unnecessary.
  • EP 0 517 119 B1 and US Pat. No. 5,458,854 A disclose such blood withdrawal devices with a collection tube for the blood.
  • the tube or the blood collection vessel has an integrated, vessel-like blood collection space with a rounded end open at the top and a rounded end closed at the bottom.
  • the open end of the container is provided in one piece with a lip part in the shape of a shovel, over the receiving edge of which the withdrawn blood can flow into the blood collection space, which is larger in diameter.
  • the upper end of the vessel together with the lip-shaped lip part formed thereon can be closed by a cap. This is attached to the lower end of the vessel while the blood is being drawn.
  • a standard clinical centrifuge according to the.
  • a shovel-shaped lip part serving for blood collection or a capillary tube is an integral part of a cap that can be plugged onto the blood collection vessel.
  • Another problem with the preparation and analysis of very small amounts of blood in the laboratory is to find such vessels that fit on the one hand due to their external dimensions in the common laboratory equipment, can be labeled (e.g. with barcode labels) and still guarantee a fill level of the sample which is still easy to pipette or handle. If vessels with an outer diameter common for commercial centrifuges and the usual wall thicknesses are used, a capillary blood volume with the volume of one or more droplets is so low that the bulk of the blood is distributed over the inner surface of the vessel and therefore almost no sample for the analysis is available. If, on the other hand, vessels with a smaller diameter guaranteeing a sufficient fill level are used, they do not fit into the commercially available devices.
  • vessels that have sufficient dimensions in their relevant upper area suitable for the devices and that taper towards the bottom are that they become Do not let the commercially available barcode labels stick on the tubes.
  • vessels are available on the market whose outer contours correspond to the required dimensions of commercially available laboratory equipment and whose inner vessel diameter is as small and narrow as possible.
  • These large dimensional differences between the outside and inside diameters are compensated for by a relatively large wall thickness.
  • the manufacture of such vessels then leads to the technical problems well known to the injection molding specialist.
  • the required, rather complex injection molding tool for the production of these vessels, which have a large wall thickness is only ever suitable for the manufacture of vessels from plastics of one type.
  • plastics of one type since different types of plastic show different behavior towards blood or other specimen, depending on the requirements for the specimen, vessels made of different types of plastic are also required. This means that each time the manufacture or use of another injection molding tool is necessary, which leads to high investments.
  • the invention has for its object to provide a sample vessel for withdrawing the smallest amounts of blood, with which the aforementioned disadvantages can be remedied and which facilitates blood withdrawal, is easier to manufacture and offers more varied possible uses.
  • a sample vessel which consists of an outer vessel and an inner vessel which can be inserted therein, which is closed at the bottom and is formed at the top, on the outlet side, with an open, very thin-walled blood collection ring which can be closed by a stopper and projects in situ from the outer vessel , wherein the outer vessel has a collar supporting the inner vessel below the blood collection ring and suitable for inclusion in centrifuges. Due to the two-part design of the sample vessel according to the invention with the all-round, very thin-walled blood collection ring of the inner vessel and the support collar of the separate outer vessel, several advantages can be achieved simultaneously.
  • the blood collection ring is also suitable as a seat for a stopper that closes the sample vessel.
  • the outer vessel can - since it is not directly involved in the blood collection - be provided in such a size that it provides a sufficiently large area for the attachment of a measuring or identification carrier (bar code).
  • the collar of the outer vessel fulfills a double function, namely it serves both to support the inner vessel that can be inserted or glued or latched into the outer vessel due to a press fit with a tight fit, and also to support when inserting it into any of the centrifuges common on the market.
  • FIG. 1 in an explosive representation the overall view of the individual parts of a first embodiment of a schematically shown sample vessel.
  • FIG. 2 shows the sample vessel according to FIG. 1 ready for taking blood;
  • FIG. 3 shows the sample vessel according to FIG. 2 in a modified version with an attachable capillary
  • a sample vessel 1 shown in FIG. 1 consists of a tubular outer vessel 2, an inner vessel 3 and a stopper 4.
  • the outer vessel 2 is provided with a collar 5, to which, in the installation position shown in FIG. 2, there is a press fit in the outer vessel 2 inserted inner vessel 3 supports.
  • the inner vessel 3 closed at the bottom has, in the embodiment shown, a conical vessel section 6 which, especially in the case of inner vessels designed with a small filling volume, nevertheless ensures a high level of the serum or plasma after centrifugation, which allows the desired amount of liquid to be pipetted off easily and simply.
  • the inner vessel 3 is formed with an extension in the form of a very thin-walled blood collection ring 7, which allows an all-round collection of the blood collected in the inner vessel 3, so that the sample vessel 1 can be placed at any point on the blood collection ring 7; it is therefore possible to draw peripheral blood easily by scooping up the escaping blood.
  • the stopper 4, which is attached to the blood collection ring 7 of the inner vessel 3 in the delivery state, can be inserted into the lower end of the outer vessel 2 during the blood uptake, as illustrated in FIG. 2 by the arrow 8.
  • the two-part sample vessel 1 also offers in a simple manner the possibility of taking blood through a capillary 9.
  • This is arranged there in a holding plug 10 which is pushed onto the blood collecting ring 7, so that the lower end of the capillary in the inner vessel 3 opens and consequently leads the drawn blood into the inner vessel 3.
  • a suitable venting of the inner vessel ensures that the blood gets into the capillary 9.
  • the stopper 10 carrying the capillary 9 closes the inner vessel 3, while a closure stopper 4, as shown in FIG. 2, not shown here, closes the lower end of the outer vessel 2 is inserted.
  • the empty capillary 9, together with the holding plug 10 is removed from the sample vessel 1 or the blood collection ring 7 of the inner vessel 3 and disposed of; the stopper 4 is then placed on the blood collection ring 7 and the sample vessel 1 is thus closed.
  • the inner vessel 3 can be designed with a flow aid 11, the embodiment of the flow aid 11 shown in FIG. 4 being a largely simplified production, eg by milling.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Centrifugal Separators (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Medical Preparation Storing Or Oral Administration Devices (AREA)

Abstract

PCT No. PCT/DE97/02712 Sec. 371 Date Aug. 18, 1998 Sec. 102(e) Date Aug. 18, 1998 PCT Filed Nov. 18, 1997 PCT Pub. No. WO98/22218 PCT Pub. Date May 28, 1998A sample vessel (1) for taking very small amounts of blood, consisting of an outer vessel (2) and an inner vessel (3) which can be inserted into it, which is closed at the bottom, and on top, on the side where the sample is deposited, is configured with an open, very thin-walled blood sample ring (7) which can be closed by a stopper (4) and which protrudes in situ from the outer vessel (2). The outer vessel (2) has a collar (5) which supports the inner vessel (3) below the blood sample ring (7), and which is suitable for holding in centrifuges. The sample vessel makes taking blood samples easier, is simpler to produce and offers a greater variety of possibilities for use.

Description

Probengefäß zur BlutabnahmeSample container for taking blood
Die Erfindung betrifft ein Probengefäß zur Abnahme sehr geringer Blutmengen, z.B. aus einer Fingerkuppe oder einem Ohrläppchen eines Patienten, so daß die Entnahme von venösem Blut häufig entbehrlich ist.The invention relates to a sample vessel for taking very small amounts of blood, e.g. from a patient's fingertip or earlobe, so that venous blood withdrawal is often unnecessary.
Durch die EP 0 517 119 B1 und die US 5 458 854 A sind solche Blutentnahmevorrichtungen mit einem Sammelröhrchen für das Blut bekanntgeworden. Das Röhrchen bzw. das Blutaufnahmegefäß besitzt einen integrierten, gefäßartigen Blutsammeiraum mit einem oben offenen und einem unten geschlossenen, gerundeten Ende. Das offene Ende des Behälters ist einstückig mit einem schaufeiförmigen Lippenteil versehen, über dessen Aufnahmerand das abgenommene Blut in den demgegenüber im Durchmesser größer bemessenen Blutsammeiraum ablaufen kann. Das obere Ende des Gefäßes samt dem daran angeformten schaufeiförmigen Lippenteil läßt sich durch eine Kappe verschließen. Diese wird während der Blutabnahme auf das untere Gefäßende aufgesteckt. Um das Blutentnahmegefäß in eine Standard-Klinikzentrifuge einsetzen zu können, wird gemäß der . US-Patentschrift an den Boden des Gefäßes ein Ergänzungsgefäß angekoppelt. Eine derartige Vorrichtung zur Abnahme sehr geringer Blutmengen ist auch aus der EP 0 072 006 B2 bekannt. Bei dieser Ausführung ist ein der Blutabnahme dienendes schaufeiförmiges Lippenteil oder ein Kapillarröhrchen ein fester Bestandteil einer auf das Blutaufnahmegefäß aufsteckbaren Kappe.EP 0 517 119 B1 and US Pat. No. 5,458,854 A disclose such blood withdrawal devices with a collection tube for the blood. The tube or the blood collection vessel has an integrated, vessel-like blood collection space with a rounded end open at the top and a rounded end closed at the bottom. The open end of the container is provided in one piece with a lip part in the shape of a shovel, over the receiving edge of which the withdrawn blood can flow into the blood collection space, which is larger in diameter. The upper end of the vessel together with the lip-shaped lip part formed thereon can be closed by a cap. This is attached to the lower end of the vessel while the blood is being drawn. In order to be able to insert the blood collection tube in a standard clinical centrifuge, according to the. US patent coupled a supplementary vessel to the bottom of the vessel. Such a device for taking very small amounts of blood is also known from EP 0 072 006 B2. In this embodiment, a shovel-shaped lip part serving for blood collection or a capillary tube is an integral part of a cap that can be plugged onto the blood collection vessel.
Diesen Blutabnahme- bzw. Blutentnahmevorrichtungen ist gemeinsam, daß sie zum Auffangen und Überleiten des Blutes in das Blutaufnahmegefäß gezielt angesetzt werden müssen, nämlich exakt mit dem schaufeiförmigen Lippenteil an der zur Blutabnahme punktierten Stelle des Patienten. Bei einer nicht präzisen Positionierung kann das Blut nicht ordnungsgemäß in das Gefäß geleitet werden mit der Folge, daß ein Teil des Blutes außen am Gefäß entlanglaufen kann. Die bei Kapillarblut ohnehin geringe Probenmenge wird daher mitunter empfindlich reduziert. Außerdem erhöht sich die Kontaminations- bzw. Injektionsgefahr für die das Blut entnehmenden Personen und das Laborpersonal beträchtlich.These blood sampling or blood sampling devices have in common that they have to be positioned in a targeted manner in order to collect and transfer the blood into the blood collection vessel, namely exactly with the lip-shaped lip part at the point of the patient punctured for blood sampling. If the positioning is not precise, the blood cannot be guided properly into the vessel, with the result that part of the blood can run along the outside of the vessel. The small amount of sample in any case with capillary blood is therefore sometimes reduced considerably. In addition, the risk of contamination or injection increases for those taking blood People and laboratory staff considerably.
Zur Vermeidung einer präzisen Positionierung und damit Vereinfachung der Blutabnahme ist es durch die US 5 038 794 A bekanntgeworden, eine Kapillare mit einem schüsselartigen Trichter auszubilden, wobei die Kapillare mit dem Trichter bzw. der Schüssel in ein Probengefäß eingeschraubt wird, das als Sammelgefäß für die abgenommene Kapillarblutmenge dient.In order to avoid precise positioning and thus simplify blood sampling, it has become known from US Pat. No. 5,038,794 A to design a capillary with a bowl-like funnel, the capillary being screwed into a sample vessel with the funnel or the bowl, which serves as a collecting vessel for the capillary blood volume used.
Aus der DE 24 39 218 A1 ist es bekannt, das Blut in ein speziell gestaltetes Kapillargefäß abzunehmen, bei dem nämlich nur das Mündungsstück die Form einer Kapillare aufweist, während der übrige Bereich so aufgeweitet ist, daß sich eine Mikropipette einsetzen läßt. Das von dem Mündungsstück abgewandte Ende, d.h. zumindest die große Öffnung der Kapillare läßt sich durch einen Stopfen verschließen, und die Kapillare kann dann, wenn notwendig, in ein im wesentlichen zylindrisches Umgefäß gesteckt werden. Statt das Blut nach dem Entfernen aus dem Kapillargefäß zu zentrifugieren, ist es möglich, das Kapillargefäß unmittelbar selbst zum Zentrifugieren zu verwenden.From DE 24 39 218 A1 it is known to draw the blood into a specially designed capillary, in which only the mouthpiece has the shape of a capillary, while the rest of the area is widened so that a micropipette can be inserted. The end facing away from the mouthpiece, i.e. at least the large opening of the capillary can be closed by a stopper, and the capillary can then, if necessary, be plugged into an essentially cylindrical vessel. Instead of centrifuging the blood after removing it from the capillary, it is possible to use the capillary itself for centrifugation directly.
Ein weiteres Problem bei der Aufbereitung und Analyse sehr kleiner Blutmengen im Labor besteht darin, solche Gefäße zu finden, die einerseits aufgrund ihrer äußeren Abmessungen in die gängigen Laborgeräte passen, etikettierbar (z.B. mit Barcode-Etiketten) sind und denoch gleichzeitig einen Füllstand der Probe gewährleisten, der noch gut zu pipettieren bzw. handhabbar ist. Wenn somit Gefäße mit einem für handelsübliche Zentrifugen gängigen Außendurchmesser und den üblichen Wandstärken verwendet werden, so hat eine Kapillarblutmenge mit dem Volumen eines bis mehrerer Tröpfchen einen so geringen Füllstand, daß die Hauptmenge des Blutes über die Gefäßinnenoberfläche verteilt ist und daher fast keine Probe mehr für die Analysen zur Verfügung steht. Werden hingegen Gefäße mit einem einen ausreichenden Füllstand gewährleistenden kleineren Durchmesser eingesetzt, so passen diese nicht in die handelsüblichen Geräte. Bei Gefäßen, die in ihrem für die Geräte geeigneten, relevanten oberen Bereich die ausreichenden Maße besitzen und sich nach unten hin verjüngen, tritt das Problem auf, daß sich diese Gefäße nicht mit den handelsüblichen Barcode-Etiketten bekleben lassen. Um diesen gegensätzlichen maßlichen Anforderungen gerecht zu werden, sind im Handel Gefäße erhältlich, deren Außenkonturen den geforderten Maßen der handelsüblichen Laborgeräte entsprechen und deren Gefäßinnendurchmesser möglichst klein und schmal ist. Diese großen maßlichen Unterschiede zwischen dem Außen- und dem Innendurchmesser werden allerdings durch eine verhältnismäßig sehr große Wanddicke ausgeglichen. Die Herstellung solcher Gefäße führt dann zu den dem Spritzgußfachmann hinlänglich bekannten technischen Problemen. Schließlich kommt noch hinzu, daß das erforderliche, recht aufwendige Spritzgußwerkzeug für die Anfertigung dieser eine große Wandstärke besitzenden Gefäße stets nur für die Herstellung von Gefäßen aus Kunststoffen einer Gattung geeignet ist. Da jedoch unterschiedliche Kunststoffgattungen ein unterschiedliches Verhalten gegenüber Blut oder anderem Probengut zeigen, werden folglich je nach Anforderungen an das Probengut auch Gefäße aus verschiedenen Kunststoffgattungen benötigt. Das bedeutet, daß jedesmal die Anfertigung bzw. Verwendung eines weiteren Spritzgußwerkzeuges notwendig ist, was zu hohen Investitionen führt.Another problem with the preparation and analysis of very small amounts of blood in the laboratory is to find such vessels that fit on the one hand due to their external dimensions in the common laboratory equipment, can be labeled (e.g. with barcode labels) and still guarantee a fill level of the sample which is still easy to pipette or handle. If vessels with an outer diameter common for commercial centrifuges and the usual wall thicknesses are used, a capillary blood volume with the volume of one or more droplets is so low that the bulk of the blood is distributed over the inner surface of the vessel and therefore almost no sample for the analysis is available. If, on the other hand, vessels with a smaller diameter guaranteeing a sufficient fill level are used, they do not fit into the commercially available devices. The problem with vessels that have sufficient dimensions in their relevant upper area suitable for the devices and that taper towards the bottom is that they become Do not let the commercially available barcode labels stick on the tubes. In order to meet these conflicting dimensional requirements, vessels are available on the market whose outer contours correspond to the required dimensions of commercially available laboratory equipment and whose inner vessel diameter is as small and narrow as possible. These large dimensional differences between the outside and inside diameters are compensated for by a relatively large wall thickness. The manufacture of such vessels then leads to the technical problems well known to the injection molding specialist. Finally, there is also the fact that the required, rather complex injection molding tool for the production of these vessels, which have a large wall thickness, is only ever suitable for the manufacture of vessels from plastics of one type. However, since different types of plastic show different behavior towards blood or other specimen, depending on the requirements for the specimen, vessels made of different types of plastic are also required. This means that each time the manufacture or use of another injection molding tool is necessary, which leads to high investments.
Der Erfindung liegt die Aufgabe zugrunde, ein Probengefäß zur Entnahme kleinster Blutmengen zu schaffen, mit dem sich die vorgenannten Nachteile beheben lassen und das die Blutentnahme erleichtert, dabei einfacher herzustellen ist und variablere Einsatzmöglichkeiten bietet.The invention has for its object to provide a sample vessel for withdrawing the smallest amounts of blood, with which the aforementioned disadvantages can be remedied and which facilitates blood withdrawal, is easier to manufacture and offers more varied possible uses.
Diese Aufgabe wird erfindungsgemäß durch ein Probengefäß gelöst, das aus einem äußeren Gefäß und einem darin einsteckbaren Innengefäß besteht, das unten geschlossen und oben, abnahmeseitig mit einem offenen, durch einen Stopfen verschließbaren, in situ aus dem äußeren Gefäß vorstehenden, sehr dünnwandigen Blutabnahmering ausgebildet ist, wobei das äußere Gefäß ein das Innengefäß unterhalb des Blutabnahmeringes abstützenden und zur Aufnahme in Zentrifugen passenden Bund aufweist. Durch die Zweiteiligkeit des erfindungsgemäßen Probengefäßes mit dem rundumlaufend gleichbleibend sehr dünnwandigen Blutabnahmering des Innengefäßes und dem Stützbund des separaten äußeren Gefäßes lassen sich mehrere Vorteile gleichzeitig erreichen. So läßt sich anders als bei einteiligen Blutgefäßen mit sehr viel geringerem Aufwand und weit weniger komplizierten Spritzgießwerkzeugen für das Innengefäß jede beliebige Form und insbesondere eine solche Gestaltung vorsehen, die eine für die gute Mischbarkeit der Proben optimierte Gefäßgeometrie aufweist und - z.B. bei einem konischen Innengefäß - einen hohen Stand des Serums oder Plasmas nach der Zentrifugation ermöglicht, was ein gutes und einfaches Abpipettieren des Überstandes gestattet. Abgesehen von der freien Materialwahl für die beiden Gefäße, lassen sich in das äußere Gefäß hinsichtlich ihres Füllvolumens verschiedene Innengefäße von z.B. 200, 300 oder 500 Mikroliter einsetzen.This object is achieved according to the invention by a sample vessel which consists of an outer vessel and an inner vessel which can be inserted therein, which is closed at the bottom and is formed at the top, on the outlet side, with an open, very thin-walled blood collection ring which can be closed by a stopper and projects in situ from the outer vessel , wherein the outer vessel has a collar supporting the inner vessel below the blood collection ring and suitable for inclusion in centrifuges. Due to the two-part design of the sample vessel according to the invention with the all-round, very thin-walled blood collection ring of the inner vessel and the support collar of the separate outer vessel, several advantages can be achieved simultaneously. So it can be different than For one-piece blood vessels with much less effort and far less complicated injection molds for the inner vessel, provide any shape and in particular a design that has a vessel geometry that is optimized for the good miscibility of the samples and - for example with a conical inner vessel - a high level of serum or plasma after centrifugation, which allows a good and easy pipetting off of the supernatant. Apart from the free choice of materials for the two vessels, various inner vessels of, for example, 200, 300 or 500 microliters can be inserted into the outer vessel with regard to their filling volume.
Die Wand des in der Einbaulage aus dem äußeren Gefäß vorstehenden Innengefäßfortsatzes, d.h. des Blutabnahmeringes vereinfacht die Handhabung ganz wesentlich, denn es braucht nicht mehr darauf geachtet zu werden, das Probengefäß gezielt anzusetzen, denn über den extrem dünnen Rand des Blutabnahmeringes läßt sich das Blut an jeder Stelle leicht abfangen, ohne ein Zentrieren des Gefäßes zu erfordern. Der Blutabnahmering eignet sich gleichzeitig als Sitz für einen das Probengefäß verschließenden Stopfen. Das äußere Gefäß läßt sich - da es an der Blutentnahme direkt nicht beteiligt ist - von solcher Größe vorsehen, daß es eine ausreichend große Fläche zur Anbringung eines Maß- bzw. Kennzeichnungsträgers (bar code) bereitstellt. Weiterhin erfüllt der Bund des äußeren Gefäßes eine Doppelfunktion, er dient nämlich sowohl der Abstützung des aufgrund einer Preßpassung mit festem Sitz in das äußere Gefäß einbringbaren oder dort einklebbaren oder einrastbaren Innengefäßes als auch der Abstützung beim Einbringen in eine beliebige der auf dem Markt gängigen Zentrifugen.The wall of the inner vascular extension protruding from the outer vessel in the installed position, i.e. of the blood collection ring simplifies handling considerably, because there is no longer any need to pay attention to placing the sample vessel in a targeted manner, because the extremely thin edge of the blood collection ring allows the blood to be easily caught at any point without requiring the vessel to be centered. The blood collection ring is also suitable as a seat for a stopper that closes the sample vessel. The outer vessel can - since it is not directly involved in the blood collection - be provided in such a size that it provides a sufficiently large area for the attachment of a measuring or identification carrier (bar code). Furthermore, the collar of the outer vessel fulfills a double function, namely it serves both to support the inner vessel that can be inserted or glued or latched into the outer vessel due to a press fit with a tight fit, and also to support when inserting it into any of the centrifuges common on the market.
Weitere Merkmale und Vorteile der Erfindung ergeben sich aus der nachfolgenden Beschreibung, in der Ausführungsbeispiele des Gegenstandes der Erfindung näher erläutert sind. Es zeigen:Further features and advantages of the invention result from the following description, in which exemplary embodiments of the subject matter of the invention are explained in more detail. Show it:
Fig. 1 in explosiver Darstellung die Gesamtansicht der Einzelteile einer ersten Ausführung eines schematisch gezeigten Probengefäßes; Fig. 2 das zur Blutabnahme bereite Probengefäß gemäß Fig. 1 ;Figure 1 in an explosive representation the overall view of the individual parts of a first embodiment of a schematically shown sample vessel. FIG. 2 shows the sample vessel according to FIG. 1 ready for taking blood;
Fig. 3 das Probengefäß gemäß Fig. 2 in einer abgewandelten Ausführung mit einer aufsteckbaren Kapillare; undFIG. 3 shows the sample vessel according to FIG. 2 in a modified version with an attachable capillary; and
Fig. 4 einen Schnitt entlang der Linie IV-IV von Fig. 3.4 shows a section along the line IV-IV of FIG. 3rd
Ein in Fig. 1 gezeigtes Probengefäß 1 besteht aus einem röhrchenförmigen äußeren Gefäß 2, einem Innengefäß 3 und einem Stopfen 4. Das äußere Gefäß 2 ist mit einem Bund 5 versehen, an den sich in der in Fig. 2 dargestellten Einbaulage das mit einem Preßsitz in das äußere Gefäß 2 eingesteckte Innengefäß 3 abstützt. Das unten geschlossene Innengefäß 3 besitzt in der gezeigten Ausführung einen konischen Gefäßabschnitt 6, der insbesondere bei mit einem geringen Füllvolumen ausgelegten Innengefäßen dennoch einen nach der Zentrifugation hohen Stand des Serums oder Plasmas gewährleistet, was ein gutes und einfaches Abpipettieren der gewünschten Flüssigkeitsmenge erlaubt.A sample vessel 1 shown in FIG. 1 consists of a tubular outer vessel 2, an inner vessel 3 and a stopper 4. The outer vessel 2 is provided with a collar 5, to which, in the installation position shown in FIG. 2, there is a press fit in the outer vessel 2 inserted inner vessel 3 supports. The inner vessel 3 closed at the bottom has, in the embodiment shown, a conical vessel section 6 which, especially in the case of inner vessels designed with a small filling volume, nevertheless ensures a high level of the serum or plasma after centrifugation, which allows the desired amount of liquid to be pipetted off easily and simply.
Das Innengefäß 3 ist mit einem Fortsatz in Form eines sehr dünnwandigen Blutabnahmeringes 7 ausgebildet, der eine Rundum-Abnahme des in dem Innengefäß 3 aufgefangenen Blutes erlaubt, so daß das Probengefäß 1 mit einer beliebigen Stelle des Blutabnahmeringes 7 angesetzt werden kann; es ist somit eine einfache periphere Blutentnahme durch Aufschaufeln des austretenden Blutes möglich. Der im Anlieferungszustand auf den Blutabnahmering 7 des Innengefäßes 3 aufgesteckte Stopfen 4 kann während der Blutaufnahme auf das untere Ende des äußeren Gefäßes 2 gesteckt werden, wie in Fig. 2 durch den Pfeil 8 verdeutlicht.The inner vessel 3 is formed with an extension in the form of a very thin-walled blood collection ring 7, which allows an all-round collection of the blood collected in the inner vessel 3, so that the sample vessel 1 can be placed at any point on the blood collection ring 7; it is therefore possible to draw peripheral blood easily by scooping up the escaping blood. The stopper 4, which is attached to the blood collection ring 7 of the inner vessel 3 in the delivery state, can be inserted into the lower end of the outer vessel 2 during the blood uptake, as illustrated in FIG. 2 by the arrow 8.
Das zweiteilige Probengefäß 1 bietet gemäß der in Fig. 3 gezeigten Abwandlung auf einfache Weise weiterhin auch die Möglichkeit einer Blutabnahme durch eine Kapillare 9. Diese ist dort in einem Haltestopfen 10 angeordnet, der auf den Blutabnahmering 7 aufgesteckt wird, so daß das untere Kapillarenende in das Innengefäß 3 mündet und das abgenommene Blut folglich in das Innengefäß 3 leitet. Eine geeignete Entlüftung des Innengefäßes sorgt hierbei dafür, daß das Blut in die Kapillare 9 gelangt- Im Anlieferungszustand verschließt der die Kapillare 9 tragende Haltestopfen 10 das Innengefäß 3, während ein wie in Fig. 2 dargestellter, hier nicht gezeigter Verschlußstopfen 4 auf das untere Ende des äußeren Gefäßes 2 gesteckt ist. Nach der Blutentnahme wird die leere Kapillare 9 zusammen mit dem Haltestopfen 10 von dem Probengefäß 1 bzw. dem Blutabnahmering 7 des Innengefäßes 3 abgenommen und entsorgt; der Stopfen 4 wird danach auf den Blutabnahmering 7 aufgesteckt und das Probengefäß 1 somit verschlossen. Zur Unterstützung des Fließens des entweder von dem Blutabnahmering 7 oder der Kapillare 9 abgenommenen und in das Innengefäß 3 geleiteten Blutes, kann das Innengefäß 3 mit einer Fließhilfe 11 ausgebildet werden, wobei die in Fig. 4 gezeigte Ausführung der Fließhilfe 11 eine weitestgehend vereinfachte Herstellung, z.B. durch Ausfräsen, ermöglicht. According to the modification shown in FIG. 3, the two-part sample vessel 1 also offers in a simple manner the possibility of taking blood through a capillary 9. This is arranged there in a holding plug 10 which is pushed onto the blood collecting ring 7, so that the lower end of the capillary in the inner vessel 3 opens and consequently leads the drawn blood into the inner vessel 3. A suitable venting of the inner vessel ensures that the blood gets into the capillary 9. In the delivery state, the stopper 10 carrying the capillary 9 closes the inner vessel 3, while a closure stopper 4, as shown in FIG. 2, not shown here, closes the lower end of the outer vessel 2 is inserted. After the blood has been drawn, the empty capillary 9, together with the holding plug 10, is removed from the sample vessel 1 or the blood collection ring 7 of the inner vessel 3 and disposed of; the stopper 4 is then placed on the blood collection ring 7 and the sample vessel 1 is thus closed. In order to support the flow of the blood either taken from the blood collection ring 7 or the capillary 9 and directed into the inner vessel 3, the inner vessel 3 can be designed with a flow aid 11, the embodiment of the flow aid 11 shown in FIG. 4 being a largely simplified production, eg by milling.

Claims

Patentanspruch:Claim:
Probengefäß (1) zur Abnahme kleinster Blutmengen, bestehend aus einem äußeren Gefäß (2) und einem darin einsteckbaren Innengefäß (3), das unten geschlossen und oben, abnahmeseitig mit einem offenen, durch einen Stopfen (4) verschließbaren, in situ aus dem äußeren Gefäß (2) vorstehenden, sehr dünnwandigen Blutabnahmering (7) ausgebildet ist, wobei das äußere Gefäß (2) einen das Innengefäß (3) unterhalb des Blutabnahmeringes (7) abstützenden und zur Aufnahme in Zentrifugen passenden Bund (5) aufweist. Sample vessel (1) for taking the smallest amount of blood, consisting of an outer vessel (2) and an inner vessel (3) that can be inserted into it, which is closed at the bottom and at the top, on the outlet side, with an open, closable by a stopper (4), in situ from the outer Vessel (2) protruding, very thin-walled blood collection ring (7) is formed, the outer vessel (2) having a collar (5) supporting the inner vessel (3) below the blood collection ring (7) and suitable for inclusion in centrifuges.
EP97949956A 1996-11-19 1997-11-18 Sample vessel for taking blood samples Expired - Lifetime EP0877652B1 (en)

Applications Claiming Priority (3)

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DE19647673A DE19647673C2 (en) 1996-11-19 1996-11-19 Sample container for taking blood
DE19647673 1996-11-19
PCT/DE1997/002712 WO1998022218A1 (en) 1996-11-19 1997-11-18 Sample vessel for taking blood samples

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EP0877652A1 true EP0877652A1 (en) 1998-11-18
EP0877652B1 EP0877652B1 (en) 2007-01-03

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EP (1) EP0877652B1 (en)
JP (1) JP3853374B2 (en)
AT (1) ATE350165T1 (en)
AU (1) AU719213B2 (en)
CA (1) CA2241792C (en)
DE (2) DE19647673C2 (en)
ES (1) ES2283030T3 (en)
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WO1998022218A1 (en) 1998-05-28
AU5308798A (en) 1998-06-10
JP2000506419A (en) 2000-05-30
US6056925A (en) 2000-05-02
JP3853374B2 (en) 2006-12-06
CA2241792A1 (en) 1998-05-28
CA2241792C (en) 2006-11-07
DE19647673A1 (en) 1998-05-28
DE59712791D1 (en) 2007-02-15
PL185194B1 (en) 2003-03-31
EP0877652B1 (en) 2007-01-03
ES2283030T3 (en) 2007-10-16
ATE350165T1 (en) 2007-01-15
DE19647673C2 (en) 2000-08-24
AU719213B2 (en) 2000-05-04
PL327663A1 (en) 1998-12-21

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