EA201692157A1 - Синтез двухцепочечных нуклеиновых кислот - Google Patents

Синтез двухцепочечных нуклеиновых кислот

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Publication number
EA201692157A1
EA201692157A1 EA201692157A EA201692157A EA201692157A1 EA 201692157 A1 EA201692157 A1 EA 201692157A1 EA 201692157 A EA201692157 A EA 201692157A EA 201692157 A EA201692157 A EA 201692157A EA 201692157 A1 EA201692157 A1 EA 201692157A1
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EA
Eurasian Patent Office
Prior art keywords
nucleic acids
present
synthesis
cellular nucleic
relates
Prior art date
Application number
EA201692157A
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English (en)
Other versions
EA035092B1 (ru
Inventor
Андрей Турчинович
Харальд Суровы
Барбара Бурвинкель
Original Assignee
Рупрехт-Карлс-Университет Гейдельберг
Дойчес Кребсфоршунгсцентрум Штифтунг Дес Оффентлихен Рехтс
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Application filed by Рупрехт-Карлс-Университет Гейдельберг, Дойчес Кребсфоршунгсцентрум Штифтунг Дес Оффентлихен Рехтс filed Critical Рупрехт-Карлс-Университет Гейдельберг
Publication of EA201692157A1 publication Critical patent/EA201692157A1/ru
Publication of EA035092B1 publication Critical patent/EA035092B1/ru

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к способу синтеза двухцепочечных нуклеиновых кислот из широкого спектра образцов и включает применение данных нуклеиновых кислот для глубокого анализа их последовательностей. Также настоящее изобретение относится к определенным реагентам, применяемым в способе согласно настоящему изобретению. Кроме того, настоящее изобретение относится к наборам, содержащим реагенты для применения в способе согласно настоящему изобретению, и к применению указанных наборов.
EA201692157A 2014-05-14 2015-05-15 Синтез двухцепочечных нуклеиновых кислот EA035092B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14168313 2014-05-14
PCT/EP2015/060777 WO2015173402A1 (en) 2014-05-14 2015-05-15 Synthesis of double-stranded nucleic acids

Publications (2)

Publication Number Publication Date
EA201692157A1 true EA201692157A1 (ru) 2017-05-31
EA035092B1 EA035092B1 (ru) 2020-04-27

Family

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Application Number Title Priority Date Filing Date
EA201692157A EA035092B1 (ru) 2014-05-14 2015-05-15 Синтез двухцепочечных нуклеиновых кислот

Country Status (7)

Country Link
US (1) US10988795B2 (ru)
EP (1) EP3143139B1 (ru)
CN (1) CN106460052B (ru)
AU (1) AU2015261440A1 (ru)
CA (1) CA2948951C (ru)
EA (1) EA035092B1 (ru)
WO (1) WO2015173402A1 (ru)

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Also Published As

Publication number Publication date
US20190032110A1 (en) 2019-01-31
CN106460052B (zh) 2021-07-16
CN106460052A (zh) 2017-02-22
CA2948951A1 (en) 2015-11-19
US10988795B2 (en) 2021-04-27
CA2948951C (en) 2023-04-04
EA035092B1 (ru) 2020-04-27
EP3143139A1 (en) 2017-03-22
EP3143139B1 (en) 2021-12-15
WO2015173402A1 (en) 2015-11-19
AU2015261440A1 (en) 2017-01-05

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