DK1117774T3 - Fremgangsmåde til diversitetsgenerering - Google Patents

Fremgangsmåde til diversitetsgenerering

Info

Publication number
DK1117774T3
DK1117774T3 DK99947775.5T DK99947775T DK1117774T3 DK 1117774 T3 DK1117774 T3 DK 1117774T3 DK 99947775 T DK99947775 T DK 99947775T DK 1117774 T3 DK1117774 T3 DK 1117774T3
Authority
DK
Denmark
Prior art keywords
gene
genes
rate
diversity
cells
Prior art date
Application number
DK99947775.5T
Other languages
English (en)
Inventor
Julian Edward Sale
Michael S Neuberger
Sarah Jane Cumbers
Original Assignee
Medical Res Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9822104.7A external-priority patent/GB9822104D0/en
Priority claimed from GBGB9901141.3A external-priority patent/GB9901141D0/en
Priority claimed from GBGB9913435.5A external-priority patent/GB9913435D0/en
Application filed by Medical Res Council filed Critical Medical Res Council
Application granted granted Critical
Publication of DK1117774T3 publication Critical patent/DK1117774T3/da

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1024In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Ceramic Products (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Treatment Of Sludge (AREA)
  • Variable-Direction Aerials And Aerial Arrays (AREA)
DK99947775.5T 1998-10-09 1999-10-08 Fremgangsmåde til diversitetsgenerering DK1117774T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9822104.7A GB9822104D0 (en) 1998-10-09 1998-10-09 Patent application
GBGB9901141.3A GB9901141D0 (en) 1999-01-19 1999-01-19 Method for generating diversity
GBGB9913435.5A GB9913435D0 (en) 1999-06-09 1999-06-09 Method for generating diversity
PCT/GB1999/003358 WO2000022111A1 (en) 1998-10-09 1999-10-08 Method for generating diversity

Publications (1)

Publication Number Publication Date
DK1117774T3 true DK1117774T3 (da) 2010-05-03

Family

ID=27269511

Family Applications (2)

Application Number Title Priority Date Filing Date
DK99947775.5T DK1117774T3 (da) 1998-10-09 1999-10-08 Fremgangsmåde til diversitetsgenerering
DK09180683.6T DK2204447T3 (da) 1998-10-09 1999-10-08 Fremgangsmåde til generering af diversitet

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK09180683.6T DK2204447T3 (da) 1998-10-09 1999-10-08 Fremgangsmåde til generering af diversitet

Country Status (12)

Country Link
US (1) US20030087236A1 (da)
EP (2) EP1117774B1 (da)
JP (1) JP4302894B2 (da)
AT (1) ATE453710T1 (da)
AU (1) AU776049B2 (da)
CA (2) CA2346927C (da)
CY (1) CY1109986T1 (da)
DE (1) DE69941876D1 (da)
DK (2) DK1117774T3 (da)
ES (2) ES2588910T3 (da)
PT (2) PT2204447T (da)
WO (1) WO2000022111A1 (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122339B2 (en) * 1998-10-09 2006-10-17 Medical Research Council Method for generating diversity
FR2833611B1 (fr) * 2001-12-18 2004-04-02 Inst Necker Procede pour l'induction de mutations somatiques et son application en proteomique
FR2833612B1 (fr) * 2001-12-18 2006-01-27 Inst Necker Procede pour l'induction maitrisee de mutations somatiques et son application en proteomique
JP4214234B2 (ja) * 2002-07-30 2009-01-28 独立行政法人理化学研究所 体細胞相同組換えの促進方法及び特異的抗体の作製方法
EP2460889B1 (en) 2002-10-11 2013-11-20 Erasmus Universiteit Rotterdam Nucleic acid amplification primers for PCR-based clonality studies of BCL2-IGH rearrangements
AU2003287780B2 (en) * 2002-12-18 2007-03-22 Anaptysbio, Inc. In vivo affinity maturation scheme
AU2002953381A0 (en) * 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
EP1568765A1 (en) * 2004-02-23 2005-08-31 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH Method for genetic diversification in gene conversion active cells
US7393923B2 (en) * 2004-11-08 2008-07-01 The Regents Of The University Of California Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same
AU2008218925A1 (en) 2007-02-20 2008-08-28 Anaptysbio, Inc. Somatic hypermutation systems
ES2628973T3 (es) 2007-05-31 2017-08-04 University Of Washington Mutagénesis inducible de genes diana
AR068767A1 (es) 2007-10-12 2009-12-02 Novartis Ag Anticuerpos contra esclerostina, composiciones y metodos de uso de estos anticuerpos para tratar un trastorno patologico mediado por esclerostina
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
GB201223053D0 (en) 2012-12-20 2013-02-06 Medical Res Council Receptor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2685004A1 (fr) * 1991-12-11 1993-06-18 Pasteur Institut Procede de generation d'une diversite structurale et fonctionnelle dans une sequence peptidique.
WO1993022457A1 (en) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Screening for genetic variation
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification

Also Published As

Publication number Publication date
DK2204447T3 (da) 2016-09-12
DE69941876D1 (de) 2010-02-11
CA2346927A1 (en) 2000-04-20
AU776049B2 (en) 2004-08-26
CY1109986T1 (el) 2014-09-10
AU6114299A (en) 2000-05-01
CA2675802A1 (en) 2000-04-20
EP1117774A1 (en) 2001-07-25
EP2204447A3 (en) 2011-05-25
JP2002527060A (ja) 2002-08-27
CA2346927C (en) 2009-09-22
US20030087236A1 (en) 2003-05-08
WO2000022111A1 (en) 2000-04-20
EP1117774B1 (en) 2009-12-30
ATE453710T1 (de) 2010-01-15
ES2588910T3 (es) 2016-11-07
ES2336982T3 (es) 2010-04-19
JP4302894B2 (ja) 2009-07-29
PT1117774E (pt) 2010-02-17
EP2204447A2 (en) 2010-07-07
PT2204447T (pt) 2016-08-31
EP2204447B1 (en) 2016-06-01

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