DE69426956D1 - Nachweis von Mycobakterien durch Nukleinsäure-Multiplexamplifizierung - Google Patents

Nachweis von Mycobakterien durch Nukleinsäure-Multiplexamplifizierung

Info

Publication number
DE69426956D1
DE69426956D1 DE69426956T DE69426956T DE69426956D1 DE 69426956 D1 DE69426956 D1 DE 69426956D1 DE 69426956 T DE69426956 T DE 69426956T DE 69426956 T DE69426956 T DE 69426956T DE 69426956 D1 DE69426956 D1 DE 69426956D1
Authority
DE
Germany
Prior art keywords
mycobacteria
detection
nucleic acid
multiplex amplification
acid multiplex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69426956T
Other languages
English (en)
Other versions
DE69426956T2 (de
Inventor
George Terrance Walker
James G Nadeau
Patricia Anne Spears
Colleen M Nycz
Daryl Dee Shank
James L Schram
Stewart Russel Jurgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of DE69426956D1 publication Critical patent/DE69426956D1/de
Publication of DE69426956T2 publication Critical patent/DE69426956T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69426956T 1993-08-24 1994-08-16 Nachweis von Mycobakterien durch Nukleinsäure-Multiplexamplifizierung Expired - Fee Related DE69426956T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/111,076 US5470723A (en) 1993-05-05 1993-08-24 Detection of mycobacteria by multiplex nucleic acid amplification

Publications (2)

Publication Number Publication Date
DE69426956D1 true DE69426956D1 (de) 2001-05-03
DE69426956T2 DE69426956T2 (de) 2001-08-16

Family

ID=22336475

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69426956T Expired - Fee Related DE69426956T2 (de) 1993-08-24 1994-08-16 Nachweis von Mycobakterien durch Nukleinsäure-Multiplexamplifizierung

Country Status (9)

Country Link
US (3) US5470723A (de)
EP (1) EP0640691B1 (de)
JP (1) JP2814422B2 (de)
AU (1) AU686169B2 (de)
BR (1) BR9403324A (de)
CA (1) CA2129690A1 (de)
DE (1) DE69426956T2 (de)
ES (1) ES2157230T3 (de)
SG (1) SG44897A1 (de)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712095A (en) * 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
US6001610A (en) * 1994-11-23 1999-12-14 Roche Diagnostics, Gmbh Method for the particularly sensitive detection of nucleic acids
CA2163393C (en) * 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
DE69633941T2 (de) * 1995-12-22 2005-11-24 Dade Behring Marburg Gmbh Nachweis von unterschieden in nukleinsäuren
US5691143A (en) * 1996-03-15 1997-11-25 Becton, Dickinson And Company Amplification and detection of the alpha antigen gene of mycobacterium avium complex species
WO1997035026A1 (en) * 1996-03-18 1997-09-25 Molecular Biology Resources, Inc. Target nucleic acid sequence amplification
US5811269A (en) * 1996-04-30 1998-09-22 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5858665A (en) * 1996-07-25 1999-01-12 Navix, Inc. Homogeneous diagnostic assay method utilizing simultaneous target and signal amplification
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
EP1021558A1 (de) * 1997-10-10 2000-07-26 President And Fellows Of Harvard College Oberflächengebundene, doppelsträngige dna-protein-arrays
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US7090975B2 (en) * 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
AU3984699A (en) * 1998-05-12 1999-11-29 Whitehead Institute For Biomedical Research Multiplex dna amplification using chimeric primers
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
EP1112385A1 (de) 1998-09-15 2001-07-04 Yale University Künstliche vektoren mit langen wiederholungssequenzen
US6326173B1 (en) 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
JP2002541475A (ja) * 1999-04-12 2002-12-03 ナノゲン／ベクトン・ディッキンソン・パートナーシップ 鎖置換増幅およびバイオエレクトロニック・マイクロチップ技術を用いる核酸配列の増幅および分離
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6531302B1 (en) 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
AU4985900A (en) * 1999-05-04 2000-11-17 Public Health Research Institute Of The City Of New York, Inc., The Secreted proteins of (mycobacterium tuberculosis) and their use as vaccines and diagnostic reagents
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US6664081B2 (en) * 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
AU2001241934A1 (en) * 2000-03-03 2001-09-17 Beckman Coulter Inc. Multiplex hybridization system for identification of pathogenic mycobacterium and method of use
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
EP2351853A1 (de) 2000-06-06 2011-08-03 Life Technologies Corporation Methode und Vorrichtungen für multiplex Amplifizierungsreaktionen
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
CA2419649C (en) 2000-09-01 2011-03-08 Gen-Probe Incorporated Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
US20020058265A1 (en) * 2000-09-15 2002-05-16 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US6844152B1 (en) * 2000-09-15 2005-01-18 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US6492120B1 (en) 2000-11-02 2002-12-10 University Of Maryland Nucleic acid hybridization assay utilizing tricyclic target and signal amplification
DE10061166A1 (de) * 2000-11-30 2002-06-06 Attomol Gmbh Molekulare Diagno Verfahren und Kit zum direkten Nachweis von Nukleotidsequenzen, Aminosäuresequenzen oder Antigenen
WO2002052043A1 (fr) * 2000-12-26 2002-07-04 Takara Bio Inc. Procede de detection d'un micro-organisme pathogene
US7101663B2 (en) * 2001-03-02 2006-09-05 University of Pittsburgh—of the Commonwealth System of Higher Education PCR method
US20020165175A1 (en) * 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
WO2002090561A1 (en) * 2001-05-07 2002-11-14 Curagen Corporation Methods and composition for nucleic acid amplification
CA2492032A1 (en) * 2001-07-15 2003-08-14 Keck Graduate Institute Gene expression analysis using nicking agents
US7112423B2 (en) * 2001-07-15 2006-09-26 Keck Graduate Institute Nucleic acid amplification using nicking agents
JP2004535815A (ja) * 2001-07-15 2004-12-02 ケック グラデュエイト インスティテュート ニック形成剤を用いた核酸フラグメントの増幅
KR100455032B1 (ko) * 2002-03-18 2004-11-06 바이오코아 주식회사 다중-이중 중합효소연쇄반응에 의한 결핵균과 비결핵균의동시 검출 방법
DE10213537A1 (de) * 2002-03-26 2003-10-30 Hain Lifescience Gmbh Verfahren und Differenzierung des Mycobacterium tuberculosis-Komplex
US20030235827A1 (en) * 2002-06-25 2003-12-25 Orchid Biosciences, Inc. Methods and compositions for monitoring primer extension and polymorphism detection reactions
CA2491117A1 (en) * 2002-06-28 2004-01-08 Orchid Biosciences, Inc. Methods and compositions for analyzing compromised samples using single nucleotide polymorphism panels
US7192700B2 (en) * 2002-12-20 2007-03-20 Orchid Cellmark Inc. Methods and compositions for conducting primer extension and polymorphism detection reactions
AU2003258012A1 (en) * 2002-08-02 2004-02-23 Orchid Cellmark Inc. Methods and compositions for genotyping
US20060073475A1 (en) * 2002-08-09 2006-04-06 Nanibhushan Dattagupta Compositions and methods for detecting pathogenic bacteria expressing chaperonin proteins
US7662594B2 (en) * 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
ATE414792T1 (de) * 2002-09-20 2008-12-15 New England Biolabs Inc Helicase-abhungige amplifikation von nukleinsuren
EP3000899A1 (de) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplexverstärkung von polynucleotiden
US20040115643A1 (en) * 2002-12-12 2004-06-17 Lizardi Paul M. Thermodynamic equilibrium extension of primers
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050053980A1 (en) 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
US20050158710A1 (en) * 2004-01-16 2005-07-21 Shirley Tsang Detection of enterovirus nucleic acid
US7481997B1 (en) 2004-02-12 2009-01-27 Montana State University Snow mountain virus genome sequence, virus-like particles and methods of use
US7229800B2 (en) 2004-04-16 2007-06-12 Becton, Dickinson And Company Neisseria gonorrhoeae assay
US8044184B2 (en) * 2004-04-26 2011-10-25 Wako Pure Chemical Industries, Ltd. Probe and primer for tubercle bacillus detection, and method of detecting human tubercle bacillus therewith
US20080311564A1 (en) * 2004-08-06 2008-12-18 Fort Thomas L Sequences and Methods for Detection of Cytomegalovirus
US20060040258A1 (en) * 2004-08-23 2006-02-23 Huiyan Guo Water-soluble conjugates and methods of preparation
KR20070085275A (ko) * 2004-09-20 2007-08-27 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 멀티플 모드에 의한 멀티플렉스 반응 억제 방법
US20060073511A1 (en) 2004-10-05 2006-04-06 Affymetrix, Inc. Methods for amplifying and analyzing nucleic acids
KR20070073917A (ko) * 2004-10-21 2007-07-10 뉴 잉글랜드 바이오랩스, 인크 개선된 증폭을 위한 핵산 복구
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US20060205090A1 (en) * 2005-03-14 2006-09-14 Newton Michael W Water-soluble conjugates for electrochemical detection
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
JP5654749B2 (ja) * 2006-04-11 2015-01-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 改良された増幅のための核酸の修復
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
JP5340167B2 (ja) 2006-12-21 2013-11-13 ジェン−プロウブ インコーポレイテッド 核酸増幅のための方法および組成物
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US20090215050A1 (en) 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20090227463A1 (en) * 2008-03-04 2009-09-10 Reif John H Autonomous in vitro evolution
US9416426B2 (en) 2008-10-27 2016-08-16 Becton, Dickinson And Company Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis pmpA gene
US8945842B2 (en) 2009-01-14 2015-02-03 Becton, Dickinson And Company Assay for Trichomonas vaginalis by amplification and detection of Trichomonas vaginalis AP65-1 gene
WO2010091111A1 (en) 2009-02-03 2010-08-12 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
WO2011003020A1 (en) 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2011047223A1 (en) 2009-10-16 2011-04-21 Monsanto Technology Llc Methods of polynucleotide detection
US9121054B2 (en) * 2009-12-08 2015-09-01 Biohelix Corporation Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
BR112013009001A2 (pt) 2010-10-12 2016-07-05 Monsanto Technology Llc planta e semente de soja correspondendo a evento transgênico mon87712 e métodos para deteção das mesmas
JP6223332B2 (ja) 2011-06-30 2017-11-01 モンサント テクノロジー エルエルシー 形質転換事象ｋｋ１７９−２に対応するアルファルファ植物および種子、ならびにその検出方法
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
ES2761920T3 (es) 2012-08-30 2020-05-21 Gen Probe Inc Amplificación de ácido nucleico multifásica
US10545161B2 (en) 2013-03-11 2020-01-28 Cue Health Inc. Systems and methods for detection and quantification of analytes
BR112015022186B1 (pt) 2013-03-11 2021-11-03 Cue Health Inc Cartucho e leitor de análise de amostras
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US9623409B2 (en) 2013-03-11 2017-04-18 Cue Inc. Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes
KR102348484B1 (ko) 2013-04-01 2022-01-07 벡톤 디킨슨 앤드 컴퍼니 인플루엔자의 진단을 위한 방법 및 키트
CA2924222C (en) 2013-10-09 2023-08-01 Monsanto Technology Llc Transgenic corn event mon87403 and methods for detection thereof
US10260111B1 (en) 2014-01-20 2019-04-16 Brett Eric Etchebarne Method of detecting sepsis-related microorganisms and detecting antibiotic-resistant sepsis-related microorganisms in a fluid sample
USD745423S1 (en) 2014-05-12 2015-12-15 Cue Inc. Automated analyzer test cartridge and sample collection device for analyte detection
WO2016149837A1 (en) 2015-03-25 2016-09-29 Axela Inc. Solid phase nucleic acid target capture and replication using strand displacing polymerases
JP6966681B2 (ja) 2015-04-24 2021-11-17 アティラ バイオシステムズ インコーポレイテッドAtila Biosystems Incorporated 限られたヌクレオチド組成を有するプライマーを用いた増幅
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
CN112881730B (zh) 2015-07-17 2024-07-12 克忧健康公司 用于增强检测和分析物定量的系统及方法
US11237161B2 (en) 2017-01-25 2022-02-01 Cue Health Inc. Systems and methods for enhanced detection and quantification of analytes
US11066691B1 (en) 2018-09-14 2021-07-20 National Technology & Engineering Solutions Of Sandia, Llc Therapeutic phages and methods thereof
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN112553350A (zh) * 2020-11-13 2021-03-26 吴涛 一种能快速检测多种分枝杆菌的方法
CN116837119A (zh) * 2022-03-25 2023-10-03 湖州申科生物技术股份有限公司 检测分枝杆菌dna的引物及检测方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
CA1339731C (en) * 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5213961A (en) * 1989-08-31 1993-05-25 Brigham And Women's Hospital Accurate quantitation of RNA and DNA by competetitive polymerase chain reaction
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
EP0533854A4 (en) * 1990-12-20 1994-06-08 Hoffmann La Roche Pcr primers for detection of legionella species and methods for controlling visual intensity in hybridization assays
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU652214B2 (en) * 1991-01-31 1994-08-18 Becton Dickinson & Company Strand displacement amplification "Sealing Clerk - sealing is to be refunded"
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
DE69233285T2 (de) * 1991-08-02 2004-11-25 bioMérieux B.V. Quantifikation von Nukleinsäuren
EP0534858B2 (de) * 1991-09-24 2005-04-27 Keygene N.V. Selektive Restriktionsfragmentenamplifikation: generelles Verfahren für DNS-Fingerprinting
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5457027A (en) 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets

Also Published As

Publication number Publication date
ES2157230T3 (es) 2001-08-16
DE69426956T2 (de) 2001-08-16
EP0640691A3 (de) 1995-07-12
AU686169B2 (en) 1998-02-05
EP0640691B1 (de) 2001-03-28
US5470723A (en) 1995-11-28
JP2814422B2 (ja) 1998-10-22
US5561044A (en) 1996-10-01
JPH07163396A (ja) 1995-06-27
US5736365A (en) 1998-04-07
EP0640691A2 (de) 1995-03-01
AU6888894A (en) 1995-03-09
CA2129690A1 (en) 1995-02-25
SG44897A1 (en) 1997-12-19
BR9403324A (pt) 1995-04-11

Similar Documents

Publication Publication Date Title
DE69426956D1 (de) Nachweis von Mycobakterien durch Nukleinsäure-Multiplexamplifizierung
DE69636843D1 (de) Nachweis von Nukleinsäure-Änderungen
DE69836885D1 (de) Nachweis von Nukleinsäuren durch Fluoreszenz quenching
DE69132905D1 (de) Detektion von Nukleinsäuresequenzen
DE3671263D1 (de) Bauelementensatz zur errichtung von bauwerken.
ATE113610T1 (de) Ester von alginsäure.
DK0566751T3 (da) DNA-påvisningsmetode
DE69434942D1 (de) Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben
DE69837125D1 (de) Nachweis von Nukleinsäuren durch Aufhebung der Fluoreszenz
DE69433180D1 (de) Felder von nukleinsaeuresonden auf biologischen chips
ATA14789A (de) Gewinnung von amphiregulin durch gentechnische verfahren
NO870613D0 (no) Deteksjon av mikroorganismer i en proeve inneholdende nukleinsyre.
DE3580057D1 (de) Heterocylische mercaptocarbonsaeureamide, -imide und -nitrile als korrosionsinhibitoren.
DE69416526D1 (de) Wiedergewinnung einer aminosäure
DK39592D0 (da) Method of detecting microorganisms
DE4496581T1 (de) Nucleinsäure-Sequenzierungsgele
DE3686376D1 (de) Signalverarbeitungsverfahren zur bestimmung der basissequenz von nukleinsaeure.
DE3671918D1 (de) Nukleinsaeure sequenzanalyse durch hemmung von exonuklease.
FI880660A0 (fi) Metod foer minskande av akrylamidhalten i vatten-i-olja-emulsioner innehaollande akrylamidpolymerer genom anvaendning av amidas.
DE3678815D1 (de) Rekristallisation von bisphenol-a durch azeotroptrocknung des loesungsmittels.
DE69231178D1 (de) Nukleinsäure-amplifikation durch polymerase-reaktion
DE3686893D1 (de) Gaerung zur gewinnung von d-milchsaeure.
DE69221641D1 (de) Quantitativer nachweis von nukleinsäure-amplifikationsprodukten
DK590587D0 (da) Detektion af nucleinsyre-hybrider
DE3689702D1 (de) Dihydrolysergsäureester.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee